HG10021448 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021448 vs. NCBI nr
Match: XP_038895208.1 (tetrahydrocannabinolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 980.7 bits (2534), Expect = 5.1e-282
Identity = 483/543 (88.95%), Postives = 511/543 (94.11%), Query Frame = 0

Query: 1   MKSFFLSTFSLTFIVLSISPSWVV-STKKHEAFLQCLLNNSPSTYSISNLIYTPINSSFY 60
           MKSF L+ F+LTFIVLSISPSWVV STKKHE F+QCLLNNS STY ISNLIYTPINSS+ 
Sbjct: 1   MKSFLLTPFALTFIVLSISPSWVVFSTKKHENFVQCLLNNSLSTYPISNLIYTPINSSYS 60

Query: 61  SVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSY 120
           S+LNFSIQN RFS+KETPKPLAIITPSHVSHIQAA+ICSKSH LQIRTRSGGHDFEGLSY
Sbjct: 61  SILNFSIQNLRFSSKETPKPLAIITPSHVSHIQAAIICSKSHALQIRTRSGGHDFEGLSY 120

Query: 121 VSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGV 180
           +SDV F+VVDLINL+SIA+DV+NE AWVQSG TLGELYYRIAEKSRTLAFPAGSCPTVGV
Sbjct: 121 ISDVPFVVVDLINLKSIAIDVENETAWVQSGATLGELYYRIAEKSRTLAFPAGSCPTVGV 180

Query: 181 GGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIV 240
           GGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 181 GGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQE 300
           VAWKVKLVRVP TVTICGINR+LE+EDTIKLI+RWQYVTNKLDENLLLGISL GGNSTQE
Sbjct: 241 VAWKVKLVRVPATVTICGINRNLEEEDTIKLIHRWQYVTNKLDENLLLGISLIGGNSTQE 300

Query: 301 GGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEAF 360
           GGKTNP ALFSSFFLGKVNEL+ IL TNF ELGLAKEDCSEMSWIKS LIMAGFPKQEA 
Sbjct: 301 GGKTNPMALFSSFFLGKVNELVTILNTNFSELGLAKEDCSEMSWIKSVLIMAGFPKQEAL 360

Query: 361 EVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIF 420
           EVLL+R P  GLSTKIKSDYVK+PIS+A+FKT+ KRLKA+DIEVAQIMFIPYGGRMSEI 
Sbjct: 361 EVLLNRAPPSGLSTKIKSDYVKEPISKASFKTMFKRLKAEDIEVAQIMFIPYGGRMSEIS 420

Query: 421 ESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYR 480
           ES TPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNW+R IYDYMTPFVSKSPRAAYNNYR
Sbjct: 421 ESLTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWVRVIYDYMTPFVSKSPRAAYNNYR 480

Query: 481 DLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLTDLKY 540
           DLDIGTNNK GKT Y+ ARVWGLKYFGKNFDRLVHVKTKIDP +FFRNEQS+PTLTD+KY
Sbjct: 481 DLDIGTNNKCGKTSYNRARVWGLKYFGKNFDRLVHVKTKIDPTNFFRNEQSIPTLTDIKY 540

Query: 541 SAI 543
           S I
Sbjct: 541 STI 543

BLAST of HG10021448 vs. NCBI nr
Match: XP_011648520.1 (cannabidiolic acid synthase [Cucumis sativus] >KAE8653301.1 hypothetical protein Csa_023300 [Cucumis sativus])

HSP 1 Score: 966.1 bits (2496), Expect = 1.3e-277
Identity = 472/544 (86.76%), Postives = 503/544 (92.46%), Query Frame = 0

Query: 1   MKSFFLSTFSLTFIVLS-ISPSWVVSTKKHEAFLQCLLNNSPST-YSISNLIYTPINSSF 60
           MKSFFL+ F+L FIVLS ISPSW VSTK HEAFLQCLLNNS +T YSISNLIYTPINSSF
Sbjct: 1   MKSFFLTPFALFFIVLSFISPSWAVSTKTHEAFLQCLLNNSLTTNYSISNLIYTPINSSF 60

Query: 61  YSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           YSVLNFSIQN RFS K+TPKPLAIITPSHVSHIQA ++CSKSH LQIR RSGGHDFEGLS
Sbjct: 61  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQATILCSKSHALQIRIRSGGHDFEGLS 120

Query: 121 YVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVG 180
           YVSDV FI+VDLINLRSI +DV+NENAWVQSG TLGE YYRI EKS+TLAFPAGSCPTVG
Sbjct: 121 YVSDVPFIIVDLINLRSITIDVENENAWVQSGATLGEFYYRIGEKSQTLAFPAGSCPTVG 180

Query: 181 VGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMG+DLFWAIRGGGGGSFGI
Sbjct: 181 IGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGDDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQ 300
           +VAWKVKLVRVP TVTICG  RSLE+EDTIKLI++WQY+TNKLD+NLLLGISL GGNSTQ
Sbjct: 241 IVAWKVKLVRVPATVTICGSQRSLEEEDTIKLIHKWQYITNKLDKNLLLGISLTGGNSTQ 300

Query: 301 EGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 360
           E GK NPTALFSSFFLGKVNELMPIL TNFPEL L+KE+CSEMSWIK+ L MAGFP QE 
Sbjct: 301 ESGKINPTALFSSFFLGKVNELMPILNTNFPELNLSKEECSEMSWIKTVLTMAGFPNQEP 360

Query: 361 FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLL+RTP FGLSTKIKSDY+KKP+SEAAFKT+LKRLKAQDIEVAQIMFIPYGGRMSEI
Sbjct: 361 FEVLLNRTPPFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAQDIEVAQIMFIPYGGRMSEI 420

Query: 421 FESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNY 480
            ES+ PFPHRAGNIYKLGYYVKWK+QSIDAEKRHLNWIRDIYDYMTPFVSKSPRA Y NY
Sbjct: 421 SESEIPFPHRAGNIYKLGYYVKWKDQSIDAEKRHLNWIRDIYDYMTPFVSKSPRATYCNY 480

Query: 481 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLTDLK 540
           RDLDIG NNKYGK  YSHARVWG KYFGKNFDRLVH+KTKIDP DFFRNEQS+P L ++K
Sbjct: 481 RDLDIGMNNKYGKATYSHARVWGFKYFGKNFDRLVHLKTKIDPNDFFRNEQSIPALKNIK 540

Query: 541 YSAI 543
           YSAI
Sbjct: 541 YSAI 544

BLAST of HG10021448 vs. NCBI nr
Match: XP_008445658.1 (PREDICTED: cannabidiolic acid synthase-like 1 [Cucumis melo])

HSP 1 Score: 954.5 bits (2466), Expect = 3.9e-274
Identity = 465/544 (85.48%), Postives = 498/544 (91.54%), Query Frame = 0

Query: 1   MKSFFLSTFSLTFIVLS-ISPSWVVSTKKHEAFLQCLLNNSPST-YSISNLIYTPINSSF 60
           MKSF L+ F+L FIVL+ +SPSW VSTKKHEAFLQCL N S +T YSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           YSVLNFSIQN RFS K+TPKPLAIITPSHVSHIQAA+ICSKSH LQIR RSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVG 180
           YVSDV FIVVDLINLRSI +DV+ ENAWVQSG TLGE YYRI EKSRTLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 VGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 VVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQ 300
           +VAWKVKLVRVP TVTICG  RSLE+EDTIKLI++WQY+TN LD+NLLLGISL GGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 EGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 360
           E GKTNP ALFSSFFLGKVNELMPIL T FPEL L+KE+CSEMSWIK+ L+MAGFP  E 
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLL+RTPLFGLSTKIKSDY+KKP+SEAAFKT+LKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 FESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNY 480
            +S+TPFPHR GNIYKLGYYVKWK+QSIDAEKRHL+WIRDIYDYMTPFVSKSPRA Y+NY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLTDLK 540
           RDLDIG NNKYGK  YSHARVWG KYFGKNFDRLVHVKT IDP DFFRNEQS+P L D+K
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542

Query: 541 YSAI 543
           YS I
Sbjct: 543 YSVI 546

BLAST of HG10021448 vs. NCBI nr
Match: KAA0047118.1 (cannabidiolic acid synthase-like 1 [Cucumis melo var. makuwa] >TYK24888.1 cannabidiolic acid synthase-like 1 [Cucumis melo var. makuwa])

HSP 1 Score: 906.7 bits (2342), Expect = 9.3e-260
Identity = 446/536 (83.21%), Postives = 479/536 (89.37%), Query Frame = 0

Query: 1   MKSFFLSTFSLTFIVLS-ISPSWVVSTKKHEAFLQCLLNNSPST-YSISNLIYTPINSSF 60
           MKSF L+ F+L FIVL+ +SPSW VSTKKHEAFLQCL N S +T YSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           YSVLNFSIQN RFS K+TPKPLAIITPSHVSHIQAA+ICSKSH LQIR RSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVG 180
           YVSDV FIVVDLINLRSI +DV+ ENAWVQSG TLGE YYRI EKSRTLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 VGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 VVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQ 300
           +VAWKVKLVRVP TVTICG  RSLE+EDTIKLI++WQY+TN LD+NLLLGISL GGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 EGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 360
           E GKTNP ALFSSFFLGKVNELMPIL T FPEL L+KE+CSEMSWIK+ L+MAGFP  E 
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLL+RTPLFGLSTKIKSDY+KKP+SEAAFKT+LKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 FESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNY 480
            +S+TPFPHR GNIYKLGYYVKWK+QSIDAEKRHL+WIRDIYDYMTPFVSKSPRA Y+NY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           RDLDIG NNKYGK  YSHARVWG KYFGKNFDR    K   D  D  R    +P +
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRKGVDKASSDTRDPSREPIPMPIM 538

BLAST of HG10021448 vs. NCBI nr
Match: XP_022152433.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 782.7 bits (2020), Expect = 2.0e-222
Identity = 381/539 (70.69%), Postives = 448/539 (83.12%), Query Frame = 0

Query: 1   MKSFFLSTFSLTF---IVLSISPSWVVSTKKHEAFLQCLLNNSPSTYSISNLIYTPINSS 60
           MKSF L+  +  F   IVLS S  W  S  KHE FLQCL  +SPS  SIS  IYTP N S
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  FYSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           + S+LNFSIQN RFST ETPKPL +I PSH+SHIQAAVICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTV 180
           SYVS + FIV+DLI+LRSI +D KN  AWVQSG TLGELYYRIA+KS+TLAFPAGSCPTV
Sbjct: 121 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 180

Query: 181 GVGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHLSGGG+G+L+RKYGLAAD+VIDA  VDANG++ +RESM +DLFW IRGGGGGSFG
Sbjct: 181 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 240

Query: 241 IVVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGN-S 300
           IVVAWK++LV VP T+T C +NR+L++ D +KLI+RWQYV +KL++NLLLGISL GG+  
Sbjct: 241 IVVAWKLRLVPVPTTLTTCTVNRTLDR-DGVKLIHRWQYVAHKLEDNLLLGISLTGGHIL 300

Query: 301 TQEGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQ 360
           TQ+GGKTNP A F ++FLGKV+EL+ I+ T FPELGL KEDC E SWI+STLI AGF   
Sbjct: 301 TQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAG 360

Query: 361 EAFEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMS 420
           E+ E LL R PL  +STKIKSDYVK+PISEA FK + +RL  QDIE+AQ+MF+PYGGRMS
Sbjct: 361 ESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMS 420

Query: 421 EIFESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYN 480
           E  ES+TP+PHRA N+YK+GY VKW+EQS  AEKRHLNW+R+IYDYM P+V+KSPRAAY 
Sbjct: 421 EFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYV 480

Query: 481 NYRDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLT 536
           NYRDLDIG NN+Y KT Y  A+VWGLKYFGKNF+RLV VKT++DP DFFR+EQS+PT +
Sbjct: 481 NYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 538

BLAST of HG10021448 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.3e-152
Identity = 265/511 (51.86%), Postives = 349/511 (68.30%), Query Frame = 0

Query: 30  EAFLQCLLNNSPSTYSISNLIYTPINSSFYSVLNFSIQNHRFSTKETPKPLAIITPSHVS 89
           E FL+C   + P+  +   L+YT  +  + S+LN +IQN RF +  TPKPL I+TPS+ S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 90  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVSDVSFIVVDLINLRSIAVDVKNENAWVQS 149
           HIQA ++CSK  GLQIRTRSGGHD EG+SY+S V F+VVDL N+ SI +DV ++ AWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 150 GTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFVD 209
           G TLGE+YY I EK+  L+FP G CPTVGVGGH SGGG+G LMR YGLAADN+IDA  V+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 210 ANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPVTVTICGINRSLEKEDTIK 269
            +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   TI  + +++E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 270 LIYRWQYVTNKLDENLLLGISLNGGNSTQEGGKTNPT--ALFSSFFLGKVNELMPILKTN 329
           L  +WQ +  K D++L+L       N T   GK   T    FSS F G V+ L+ ++  +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 330 FPELGLAKEDCSEMSWIKSTLIMAGFPKQEAF----EVLLDRTPLFGLSTKIKSDYVKKP 389
           FPELG+ K DC E SWI +T+  +G           E+LLDR+     +  IK DYVKKP
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVKKP 391

Query: 390 ISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQTPFPHRAGNIYKLGYYVKWKE 449
           I E A   +L++L  +D+     +  PYGG M EI ES  PFPHRAG +Y+L Y   W++
Sbjct: 392 IPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASWEK 451

Query: 450 QSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRDLDIGTNNKYGKTIYSHARVWGLK 509
           Q  +  ++H+NW+R +Y++ TP+VS++PR AY NYRDLD+G  N      Y+ AR+WG K
Sbjct: 452 Q--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARIWGEK 511

Query: 510 YFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           YFGKNF+RLV VKTK+DP +FFRNEQS+P L
Sbjct: 512 YFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of HG10021448 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-152
Identity = 277/540 (51.30%), Postives = 367/540 (67.96%), Query Frame = 0

Query: 7   STFSLTFI--VLSISPSWVVST---KKHEAFLQCLLNNSPSTYSISNLIYTPINSSFYSV 66
           STFS  F+  ++    S+ + T      E FL+C     P+  +   L+YT  N  + SV
Sbjct: 4   STFSFWFVCKIIFFFFSFNIQTSIANPRENFLKCFSQYIPNNATNLKLVYTQNNPLYMSV 63

Query: 67  LNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVS 126
           LN +I N RF++  TPKPL I+TPSHVSHIQ  ++CSK  GLQIRTRSGGHD EG+SY+S
Sbjct: 64  LNSTIHNLRFTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYIS 123

Query: 127 DVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGVGG 186
            V F++VDL N+RSI +DV ++ AWV++G TLGE+YY + EK+  L+  AG CPTV  GG
Sbjct: 124 QVPFVIVDLRNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGG 183

Query: 187 HLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVA 246
           H  GGG+G LMR YGLAADN+IDA  V+ +GKV DR+SMGEDLFWA+RGGG  SFGI+VA
Sbjct: 184 HFGGGGYGPLMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVA 243

Query: 247 WKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQEGG 306
           WK++LV VP + T+  + + +E  + +KL+ +WQ +  K D++LLL       N T   G
Sbjct: 244 WKIRLVAVPKS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQG 303

Query: 307 KTNPTAL---FSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 366
           K N TA+   FSS FLG V+ L+ ++  +FPELG+ K DC ++SWI + +  +G    + 
Sbjct: 304 K-NKTAIHTYFSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDT 363

Query: 367 ----FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGR 426
                E+LLDR+     + KIK DYVKKPI E+ F  +L++L  +DI        PYGG 
Sbjct: 364 DNFNKEILLDRSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGI 423

Query: 427 MSEIFESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAA 486
           M EI ES  PFPHRAG +Y+L Y   W++Q  +  ++HLNWIR+IY++MTP+VSK+PR A
Sbjct: 424 MDEISESAIPFPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLA 483

Query: 487 YNNYRDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           Y NYRDLDIG N+      Y+ AR+WG KYFGKNFDRLV VKT +DP +FFRNEQS+P L
Sbjct: 484 YLNYRDLDIGINDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539

BLAST of HG10021448 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 2.3e-152
Identity = 261/511 (51.08%), Postives = 353/511 (69.08%), Query Frame = 0

Query: 30  EAFLQCLLNNSPSTYSISNLIYTPINSSFYSVLNFSIQNHRFSTKETPKPLAIITPSHVS 89
           E FL+C     P+  + + L+YT  +  + S+LN +IQN RF++  TPKPL IITP +VS
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 90  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVSDVSFIVVDLINLRSIAVDVKNENAWVQS 149
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY+S V F++VDL N+ S+ +DV ++ AWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 150 GTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFVD 209
           G TLGE+YY I E +  L+FPAG CPTVG GGH SGGG+G LMR YGLAADN+IDA  V+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 210 ANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPVTVTICGINRSLEKEDTIK 269
            +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP   TI  + +++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 270 LIYRWQYVTNKLDENLLLGISLNGGNSTQEGGKTNPT--ALFSSFFLGKVNELMPILKTN 329
           L+ +WQ +    ++ LLL       N T   GK   T  + FSS F G V+ L+ ++  +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 330 FPELGLAKEDCSEMSWIKSTLIMAGFPKQEAF----EVLLDRTPLFGLSTKIKSDYVKKP 389
           FPELG+ K DC ++SWI + +  +G           E+LLDR+     +  IK DYVKKP
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 390 ISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQTPFPHRAGNIYKLGYYVKWKE 449
           I E A  T+L++L  +D+ V   +F PYGG M EI ES  PFPHRAG +Y++ Y   W++
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIASWEK 451

Query: 450 QSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRDLDIGTNNKYGKTIYSHARVWGLK 509
           Q  +  ++H+NWIR++Y++ TP+VS++PR AY NYRDLD+G  N      Y+ AR+WG K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 510 YFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           YFGKNF+RLV VKTK+DP +FFRNEQS+P L
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of HG10021448 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.0e-152
Identity = 261/511 (51.08%), Postives = 352/511 (68.88%), Query Frame = 0

Query: 30  EAFLQCLLNNSPSTYSISNLIYTPINSSFYSVLNFSIQNHRFSTKETPKPLAIITPSHVS 89
           E FL+CL    P+  + + L+YT  +  + S+LN ++QN RF++  TPKPL I TP +VS
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 90  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVSDVSFIVVDLINLRSIAVDVKNENAWVQS 149
           HIQ  ++CSK  GLQIRTRSGGHD EG+SY+S V F++VDL N+ S+ +DV ++ AWV+S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 150 GTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFVD 209
           G TLGE+YY I E +  L+FPAG CPTVG GGH SGGG+G LMR YGLAADN+IDA  V+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 210 ANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPVTVTICGINRSLEKEDTIK 269
            +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP   TI  + +++E  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 270 LIYRWQYVTNKLDENLLLGISLNGGNSTQEGGKTNPT--ALFSSFFLGKVNELMPILKTN 329
           L+ +WQ +    ++ LLL       N T   GK   T  + FSS F G V+ L+ ++  +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 330 FPELGLAKEDCSEMSWIKSTLIMAGFPKQEAF----EVLLDRTPLFGLSTKIKSDYVKKP 389
           FPELG+ K DC ++SWI + +  +G           E+LLDR+     +  IK DYVKKP
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLDYVKKP 391

Query: 390 ISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQTPFPHRAGNIYKLGYYVKWKE 449
           I E A  T+L++L  +D+ V   +F PYGG M EI ES  PFPHRAG  Y++ Y   W++
Sbjct: 392 IPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIASWEK 451

Query: 450 QSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRDLDIGTNNKYGKTIYSHARVWGLK 509
           Q  +  ++H+NWIR++Y++ TP+VS++PR AY NYRDLD+G  N      Y+ AR+WG K
Sbjct: 452 Q--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARIWGEK 511

Query: 510 YFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           YFGKNF+RLV VKTK+DP +FFRNEQS+P L
Sbjct: 512 YFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of HG10021448 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 3.3e-151
Identity = 263/511 (51.47%), Postives = 348/511 (68.10%), Query Frame = 0

Query: 30  EAFLQCLLNNSPSTYSISNLIYTPINSSFYSVLNFSIQNHRFSTKETPKPLAIITPSHVS 89
           E FL+C     P+  +    IYT  +  + SVLN +IQN RF++  TPKPL I+TPS+VS
Sbjct: 32  ENFLKCFSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNVS 91

Query: 90  HIQAAVICSKSHGLQIRTRSGGHDFEGLSYVSDVSFIVVDLINLRSIAVDVKNENAWVQS 149
           HIQA+++CSK  GLQIRTRSGGHD EGLSY+S V F +VDL N+ ++ VD+ ++ AWV++
Sbjct: 92  HIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVEA 151

Query: 150 GTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLSGGGFGWLMRKYGLAADNVIDASFVD 209
           G TLGE+YY I E +   +FP G CPTVGVGGH SGGG+G LMR YGLAADN+IDA  V+
Sbjct: 152 GATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 210 ANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVRVPVTVTICGINRSLEKEDTIK 269
            +GKV DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   TI  + +++E    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLVK 271

Query: 270 LIYRWQYVTNKLDENLLLGISLNGGNSTQEGGKTNPT--ALFSSFFLGKVNELMPILKTN 329
           L  +WQ +  K D++L+L       N T   GK   T    FSS FLG V+ L+ ++  +
Sbjct: 272 LFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNKS 331

Query: 330 FPELGLAKEDCSEMSWIKSTLIMAGFPKQEAF----EVLLDRTPLFGLSTKIKSDYVKKP 389
           FPELG+ K DC E+SWI +T+  +G           E+LLDR+     +  IK DYVKK 
Sbjct: 332 FPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVKKL 391

Query: 390 ISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQTPFPHRAGNIYKLGYYVKWKE 449
           I E A   +L++L  +++ V   +  PYGG M EI ES  PFPHRAG +Y+L Y   W++
Sbjct: 392 IPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATWEK 451

Query: 450 QSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRDLDIGTNNKYGKTIYSHARVWGLK 509
           Q  +  ++H+NW+R +Y++ TP+VS++PR AY NYRDLD+G  N      Y+ AR+WG K
Sbjct: 452 Q--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPESPNNYTQARIWGEK 511

Query: 510 YFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           YFGKNF+RLV VKTK DP +FFRNEQS+P L
Sbjct: 512 YFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540

BLAST of HG10021448 vs. ExPASy TrEMBL
Match: A0A1S3BE36 (cannabidiolic acid synthase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488617 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.9e-274
Identity = 465/544 (85.48%), Postives = 498/544 (91.54%), Query Frame = 0

Query: 1   MKSFFLSTFSLTFIVLS-ISPSWVVSTKKHEAFLQCLLNNSPST-YSISNLIYTPINSSF 60
           MKSF L+ F+L FIVL+ +SPSW VSTKKHEAFLQCL N S +T YSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           YSVLNFSIQN RFS K+TPKPLAIITPSHVSHIQAA+ICSKSH LQIR RSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVG 180
           YVSDV FIVVDLINLRSI +DV+ ENAWVQSG TLGE YYRI EKSRTLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 VGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 VVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQ 300
           +VAWKVKLVRVP TVTICG  RSLE+EDTIKLI++WQY+TN LD+NLLLGISL GGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 EGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 360
           E GKTNP ALFSSFFLGKVNELMPIL T FPEL L+KE+CSEMSWIK+ L+MAGFP  E 
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLL+RTPLFGLSTKIKSDY+KKP+SEAAFKT+LKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 FESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNY 480
            +S+TPFPHR GNIYKLGYYVKWK+QSIDAEKRHL+WIRDIYDYMTPFVSKSPRA Y+NY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLTDLK 540
           RDLDIG NNKYGK  YSHARVWG KYFGKNFDRLVHVKT IDP DFFRNEQS+P L D+K
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRLVHVKTMIDPNDFFRNEQSIPALIDIK 542

Query: 541 YSAI 543
           YS I
Sbjct: 543 YSVI 546

BLAST of HG10021448 vs. ExPASy TrEMBL
Match: A0A5D3DMS2 (Cannabidiolic acid synthase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5482G00040 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.5e-260
Identity = 446/536 (83.21%), Postives = 479/536 (89.37%), Query Frame = 0

Query: 1   MKSFFLSTFSLTFIVLS-ISPSWVVSTKKHEAFLQCLLNNSPST-YSISNLIYTPINSSF 60
           MKSF L+ F+L FIVL+ +SPSW VSTKKHEAFLQCL N S +T YSISNLIYTPINSSF
Sbjct: 3   MKSFLLTPFALFFIVLTFLSPSWAVSTKKHEAFLQCLFNKSLTTNYSISNLIYTPINSSF 62

Query: 61  YSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120
           YSVLNFSIQN RFS K+TPKPLAIITPSHVSHIQAA+ICSKSH LQIR RSGGHDFEGLS
Sbjct: 63  YSVLNFSIQNLRFSRKQTPKPLAIITPSHVSHIQAAIICSKSHALQIRIRSGGHDFEGLS 122

Query: 121 YVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVG 180
           YVSDV FIVVDLINLRSI +DV+ ENAWVQSG TLGE YYRI EKSRTLAFPAGSCPTVG
Sbjct: 123 YVSDVPFIVVDLINLRSITIDVEKENAWVQSGATLGEFYYRIGEKSRTLAFPAGSCPTVG 182

Query: 181 VGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHLSGGGFGWLMRKYGLAADNVIDASF+DANGKVYDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 183 IGGHLSGGGFGWLMRKYGLAADNVIDASFIDANGKVYDRESMGEDLFWAIRGGGGGSFGI 242

Query: 241 VVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQ 300
           +VAWKVKLVRVP TVTICG  RSLE+EDTIKLI++WQY+TN LD+NLLLGISL GGN TQ
Sbjct: 243 IVAWKVKLVRVPATVTICGSKRSLEEEDTIKLIHQWQYITNTLDKNLLLGISLTGGNLTQ 302

Query: 301 EGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 360
           E GKTNP ALFSSFFLGKVNELMPIL T FPEL L+KE+CSEMSWIK+ L+MAGFP  E 
Sbjct: 303 ESGKTNPNALFSSFFLGKVNELMPILDTKFPELNLSKEECSEMSWIKTVLVMAGFPTHEP 362

Query: 361 FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEI 420
           FEVLL+RTPLFGLSTKIKSDY+KKP+SEAAFKT+LKRLKA+DIEVAQIMFIPYGGRMSEI
Sbjct: 363 FEVLLNRTPLFGLSTKIKSDYIKKPMSEAAFKTMLKRLKAEDIEVAQIMFIPYGGRMSEI 422

Query: 421 FESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNY 480
            +S+TPFPHR GNIYKLGYYVKWK+QSIDAEKRHL+WIRDIYDYMTPFVSKSPRA Y+NY
Sbjct: 423 SKSETPFPHRVGNIYKLGYYVKWKDQSIDAEKRHLSWIRDIYDYMTPFVSKSPRATYSNY 482

Query: 481 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           RDLDIG NNKYGK  YSHARVWG KYFGKNFDR    K   D  D  R    +P +
Sbjct: 483 RDLDIGINNKYGKATYSHARVWGFKYFGKNFDRKGVDKASSDTRDPSREPIPMPIM 538

BLAST of HG10021448 vs. ExPASy TrEMBL
Match: A0A6J1DEU9 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020168 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 9.8e-223
Identity = 381/539 (70.69%), Postives = 448/539 (83.12%), Query Frame = 0

Query: 1   MKSFFLSTFSLTF---IVLSISPSWVVSTKKHEAFLQCLLNNSPSTYSISNLIYTPINSS 60
           MKSF L+  +  F   IVLS S  W  S  KHE FLQCL  +SPS  SIS  IYTP N S
Sbjct: 1   MKSFPLNPLAFAFACSIVLSSSLFWAASAHKHEGFLQCLTRHSPSNNSISKFIYTPANFS 60

Query: 61  FYSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGL 120
           + S+LNFSIQN RFST ETPKPL +I PSH+SHIQAAVICSK+HGLQIRTRSGGHDFEGL
Sbjct: 61  YSSILNFSIQNLRFSTAETPKPLLVIAPSHISHIQAAVICSKTHGLQIRTRSGGHDFEGL 120

Query: 121 SYVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTV 180
           SYVS + FIV+DLI+LRSI +D KN  AWVQSG TLGELYYRIA+KS+TLAFPAGSCPTV
Sbjct: 121 SYVSHLPFIVLDLIHLRSITIDAKNSTAWVQSGATLGELYYRIAQKSQTLAFPAGSCPTV 180

Query: 181 GVGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFG 240
           GVGGHLSGGG+G+L+RKYGLAAD+VIDA  VDANG++ +RESM +DLFW IRGGGGGSFG
Sbjct: 181 GVGGHLSGGGYGYLIRKYGLAADHVIDAHLVDANGRILNRESMEDDLFWTIRGGGGGSFG 240

Query: 241 IVVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGN-S 300
           IVVAWK++LV VP T+T C +NR+L++ D +KLI+RWQYV +KL++NLLLGISL GG+  
Sbjct: 241 IVVAWKLRLVPVPTTLTTCTVNRTLDR-DGVKLIHRWQYVAHKLEDNLLLGISLTGGHIL 300

Query: 301 TQEGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQ 360
           TQ+GGKTNP A F ++FLGKV+EL+ I+ T FPELGL KEDC E SWI+STLI AGF   
Sbjct: 301 TQQGGKTNPVASFPTYFLGKVDELVAIVNTTFPELGLTKEDCIETSWIESTLITAGFSAG 360

Query: 361 EAFEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMS 420
           E+ E LL R PL  +STKIKSDYVK+PISEA FK + +RL  QDIE+AQ+MF+PYGGRMS
Sbjct: 361 ESLETLLYRRPLVNISTKIKSDYVKEPISEAIFKGIWERLNTQDIELAQVMFVPYGGRMS 420

Query: 421 EIFESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYN 480
           E  ES+TP+PHRA N+YK+GY VKW+EQS  AEKRHLNW+R+IYDYM P+V+KSPRAAY 
Sbjct: 421 EFSESETPYPHRAENMYKMGYIVKWEEQSAKAEKRHLNWVREIYDYMAPYVTKSPRAAYV 480

Query: 481 NYRDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLT 536
           NYRDLDIG NN+Y KT Y  A+VWGLKYFGKNF+RLV VKT++DP DFFR+EQS+PT +
Sbjct: 481 NYRDLDIGRNNEYEKTNYERAKVWGLKYFGKNFNRLVQVKTRVDPSDFFRHEQSIPTFS 538

BLAST of HG10021448 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 762.7 bits (1968), Expect = 1.0e-216
Identity = 380/534 (71.16%), Postives = 438/534 (82.02%), Query Frame = 0

Query: 6   LSTFSLTFIVLSISPSWVVS-TKKHEAFLQCLLNNS--PSTYSISNLIYTPINSSFYSVL 65
           L + SL FI LS S  W  +   KHE+FLQCLL++S   S YSIS +I+TPINSS+ SVL
Sbjct: 7   LISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVL 66

Query: 66  NFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVSD 125
           +FSI+N RFST ETPKPL I+TPSHVSHIQAAVICSKS+G QIRTRSGGHDFEGLSYV+ 
Sbjct: 67  DFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAH 126

Query: 126 VSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGH 185
           + FI+VDLINLRSI++D+K+  AWVQSG TLGELYY IA+KSRTLAFPAG CPTVGVGGH
Sbjct: 127 LPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGH 186

Query: 186 LSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAW 245
            SGGG+G L+RKYGLAADNVIDA  VDA+GK ++RESMGEDLFWAIRGGGGGSFGIVVAW
Sbjct: 187 FSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAW 246

Query: 246 KVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNST-QEGG 305
           KVKLV VP TVT+C  NR+LE E  +K+ +RWQYV NKLDENL LGI   GG  T QEGG
Sbjct: 247 KVKLVPVPATVTLCSANRTLE-EGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGG 306

Query: 306 KTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIM-AGFPKQEAFE 365
           KTNP A F S FLGK +EL+ IL T FPEL LAK+DC E SWI+ST++M  GFP +   E
Sbjct: 307 KTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLE 366

Query: 366 VLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFE 425
            LL RTPL   S+KIKSDYVK+P+SEA  + + KRL +QDIE   I+F+PYGGRMS+I E
Sbjct: 367 ALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISE 426

Query: 426 SQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRD 485
           S+TPF HRAG +YK+GY   W+EQ IDAEKRHL WIR++YDYMTPFVSKSPRAAY NYRD
Sbjct: 427 SETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRD 486

Query: 486 LDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           LDIG+NNK+GKT Y  A VWGLKYFG NF+RLVHVKTK+DP DFFR+EQS+P L
Sbjct: 487 LDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of HG10021448 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.2e-215
Identity = 377/528 (71.40%), Postives = 430/528 (81.44%), Query Frame = 0

Query: 11  LTFIVLSISPSWVVS-TKKHEAFLQCLLNNSP-STYSISNLIYTPINSSFYSVLNFSIQN 70
           L FI LS S  W ++   KHE+FLQCLLN+SP   YSIS +I+TPINSS+ SVL+FSI+N
Sbjct: 14  LAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSSYSSVLDFSIRN 73

Query: 71  HRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVSDVSFIVV 130
            RFST ETPKPL IITPSHVSHIQAAV+CSK +G QIRTRSGGHDFE LSYV+ +SFI+V
Sbjct: 74  LRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSYVAHLSFIIV 133

Query: 131 DLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLSGGGF 190
           DLINLRSI+VDVKN NAWV SG TLGELYY IA+KSRT AFPAG CPTVGVGGH SGGG+
Sbjct: 134 DLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGVGGHFSGGGY 193

Query: 191 GWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVR 250
           G L+RKYGLAADNVIDA  VDANGK +DRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 
Sbjct: 194 GLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 253

Query: 251 VPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNST-QEGGKTNPTA 310
           VP TVT+C  NR+LE E  +KL +RWQYV NKLDENL LGI   GGN T Q GGKTNP A
Sbjct: 254 VPATVTLCQTNRTLE-EGAVKLTHRWQYVANKLDENLFLGILWLGGNVTSQGGGKTNPVA 313

Query: 311 LFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMA-GFPKQEAFEVLLDRT 370
            F S FLG+ +EL+ IL T FPEL LAK+DC E SWI+ST++M  GF  +   E LL RT
Sbjct: 314 TFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGFQTKVTLEALLSRT 373

Query: 371 PLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQTPFP 430
           PL  +STKIKSDYVK+PISEA  + + +RL AQDIE   ++F+PYGGRMS+IFES+TPF 
Sbjct: 374 PLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFVPYGGRMSQIFESETPFS 433

Query: 431 HRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRDLDIGTN 490
           HRAG +YK+GY   W +QSID EKRHL+WIR++Y YM PFVSKSPRA Y NYRDLDIG+N
Sbjct: 434 HRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSKSPRAVYANYRDLDIGSN 493

Query: 491 NKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
            +YGKT Y  A  WG KYFG NF+RLVHVKTK+DP DFFR+EQS+PTL
Sbjct: 494 KRYGKTSYKQASTWGFKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 540

BLAST of HG10021448 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 523.5 bits (1347), Expect = 2.1e-148
Identity = 259/533 (48.59%), Postives = 368/533 (69.04%), Query Frame = 0

Query: 7   STFSLTFIVLSISPSWVVST--KKHEAFLQCLLNNSPSTYSISNLIYTPINSSFYSVLNF 66
           S+F  T ++ S++ S +VS     HE FL+CL            +I+T  +SSF+S+L+ 
Sbjct: 4   SSFLFTILLFSLNISPLVSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDS 63

Query: 67  SIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVS-DV 126
           SIQN RFS  ETPKP++IITP   S +Q  + C++ HG+ +RTRS GH +EGLSY++ + 
Sbjct: 64  SIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNK 123

Query: 127 SFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHL 186
            F V+DL NLRSI++DV N   WVQ+G T GELYY I + +++LAFPAG  PTVGVGG  
Sbjct: 124 PFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQF 183

Query: 187 SGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWK 246
           SGGG+G L+RKYGLAADN+IDA  VDA+G++ DR++MGED FWAIRGGGG SFG++++WK
Sbjct: 184 SGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWK 243

Query: 247 VKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQEGGKT 306
           VKLV VP T+T+  + ++ +KE  +++I +WQY  +K+ ++L +  +L   N      K 
Sbjct: 244 VKLVDVPSTITVFKVQKTSKKE-AVRIIKKWQYAADKVPDDLFIRTTLERSN------KN 303

Query: 307 NPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEAFEVLL 366
              ALF+  ++G VN L+ +++  FPELGL KE C EMSWI+S L  A FPK E+  VL 
Sbjct: 304 AVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKGESLGVLT 363

Query: 367 DRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQT 426
           +R     LS K K D+V++PI EAA + + +RL+A +  + +I+  P+GG+MSE+ E +T
Sbjct: 364 NRERT-SLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYET 423

Query: 427 PFPHRAGNIYKLGYYVKWKEQSIDAEK----RHLNWIRDIYDYMTPFVSKSPRAAYNNYR 486
           PFPHR GN+Y++ Y   W+E+  D  K    ++L W+  +Y++MTP+VSKSPR AY N++
Sbjct: 424 PFPHRGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFK 483

Query: 487 DLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVP 533
           D+D+G      KT Y   + WG+KYF  NF+RLV VKT++DP DFF +EQS+P
Sbjct: 484 DMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of HG10021448 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 1.4e-144
Identity = 256/537 (47.67%), Postives = 361/537 (67.23%), Query Frame = 0

Query: 2   KSFFLST---FSLTFIVLSISPSWVVSTKKHEAFLQCLLNNSPSTYSISNLIYTPINSSF 61
           +SFF S    F+ + ++LSI      +     +FLQCL      +  +S +I+TP ++SF
Sbjct: 4   QSFFSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQLNDSNIVSKVIHTPNDTSF 63

Query: 62  YSVLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 121
            SVL  SIQN RFS  + PKP+ I+TP   S +Q+AV C++  G+ IRTRSGGHD+EGLS
Sbjct: 64  SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLS 123

Query: 122 YVSDVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVG 181
           YV+   F+++DL NLRSI VDV N + WVQ+G T+GELYY I +K+RTLAFPAG CPTVG
Sbjct: 124 YVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVG 183

Query: 182 VGGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGI 241
           VGGH SGGG+G L+RK+GLAAD+VIDA  VDA G++ +R  MGED FWAIRGGGG SF +
Sbjct: 184 VGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCV 243

Query: 242 VVAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQ 301
           V++WK+ L+ VP TVT+  + +   ++  +K+I+RWQ+V +K+ ++L + + L       
Sbjct: 244 VLSWKIGLINVPSTVTVFNVTK-FSEQSALKIIHRWQFVADKVSDDLFIRVML------- 303

Query: 302 EGGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEA 361
           +  K    A F   +LG V  L+ ++   FPELGL ++DC+EMSWI+S +  A    +E 
Sbjct: 304 QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAEL-GEEP 363

Query: 362 FEVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEI 421
             VL  RT    L+ K KSD+V++P+ + A   L +RL+  + E AQ++F P+GG+MSEI
Sbjct: 364 INVLTKRTRA-SLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEI 423

Query: 422 FESQTPFPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNY 481
            + +TPFPHR GNIY++ Y   W+    D +++++ W+  +YD M+ FV+KSPR AY N 
Sbjct: 424 ADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYINL 483

Query: 482 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTLT 536
           RDLD+G      ++ Y   + WG+KYF  NF+RLV VKT +DP DFF +EQS+P  T
Sbjct: 484 RDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPPFT 527

BLAST of HG10021448 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 505.4 bits (1300), Expect = 5.8e-143
Identity = 254/536 (47.39%), Postives = 361/536 (67.35%), Query Frame = 0

Query: 7   STFSLTFIVLSISPSWVVSTKKHEAFLQCLLNNSPSTYSISNLIYTPIN--SSFYSVLNF 66
           S   L F + +I+P+  +++ + + F+ C+  N+   + +    + P    S F  VL  
Sbjct: 11  SILVLYFSLYTITPTSSLASLQ-DQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLES 70

Query: 67  SIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVS--D 126
           + QN RF  K  PKP  I +P H SH+QA++ICSK   + +R RSGGHD+EGLSYVS  D
Sbjct: 71  TAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQID 130

Query: 127 VSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGH 186
             FI++DL  +R + +++++ +AWVQSG T+GELYYRIAEKS+   FPAG C ++G+GGH
Sbjct: 131 KPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGH 190

Query: 187 LSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAW 246
           ++GG +G +MRKYGL ADNV+DA  VDANGK+ DR +MGED FWAIRGG GGSFGI++AW
Sbjct: 191 ITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAW 250

Query: 247 KVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQEGGK 306
           K+KLV VP TVT+  + ++L+++   K+I +WQ V +KL E L + +  N   +   GG 
Sbjct: 251 KIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT---GGN 310

Query: 307 TNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEAFEVL 366
              T  +++ FLG    LM ++K +FPELGL  +DC EMSW++S   ++GFP      VL
Sbjct: 311 KTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVL 370

Query: 367 LD-RTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFES 426
           L  ++P   +S K KSD+VK PI E+  + + K+L  +DI +  +++ PYGG M++I ES
Sbjct: 371 LQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPES 430

Query: 427 QTPFPHRAGNIYKLGYYVKWKEQSIDAEK---RHLNWIRDIYDYMTPFVSKSPRAAYNNY 486
           Q PFPHR G ++K+ Y   W    +D++K   RH+NWIRD+Y YMTP+VS +PR AY NY
Sbjct: 431 QIPFPHRKGVLFKVQYVTSW----LDSDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNY 490

Query: 487 RDLDIGTNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
           RDLD+G N K  KT    A+VWG  YF  NF+RL+ +K K+DP +FFR+EQS+P +
Sbjct: 491 RDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536

BLAST of HG10021448 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 499.2 bits (1284), Expect = 4.2e-141
Identity = 245/529 (46.31%), Postives = 364/529 (68.81%), Query Frame = 0

Query: 9   FSLTFIVLSISPSWVVSTKKHEAFLQCLLNNSPSTYSISNLIYTPINSS--FYSVLNFSI 68
           F +T +++S+  S   S+   + F++CL++NS  ++ I+   ++P  ++  F   L  + 
Sbjct: 13  FLVTLLLISVPLS---SSTLQQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEELESTA 72

Query: 69  QNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVS--DVS 128
           QN R+ T   PKP+ I  P + +H+QAAV+C+K   L +R RSGGHD+EGLS+V+  +  
Sbjct: 73  QNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAEDETP 132

Query: 129 FIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGVGGHLS 188
           F++VDL  LR + VD+ + +AW  +G T+GE+YYRI EKS+T  FPAG C ++G+GGHL 
Sbjct: 133 FVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLV 192

Query: 189 GGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIVVAWKV 248
           GG +G +MRK+GL ADNV+DA  VDANG++ DR +MGED+FWAIRGGGGGSFG+++AWK+
Sbjct: 193 GGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKI 252

Query: 249 KLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQEGGKTN 308
           KLV VP TVT+  + ++LE++ T K++Y+W+ + +KLD++L + + ++  + T + G   
Sbjct: 253 KLVPVPATVTVFTVTKTLEQDGT-KVLYKWEQIADKLDDDLFIRVIISPASKTTKPGNRT 312

Query: 309 PTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEAFEVLLD 368
            +  + + FLG  N L+ +++ +FPELGL K+DC+EMSWIKS + +AGFP   A E LL 
Sbjct: 313 ISMSYQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLA 372

Query: 369 RTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIFESQTP 428
              LF    K KSD+VK+PI     + L +R   +D  +   ++ PYGG MS I ES+ P
Sbjct: 373 GKSLFKNHFKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEIP 432

Query: 429 FPHRAGNIYKLGYYVKWKEQSIDAEKRHLNWIRDIYDYMTPFVSKSPRAAYNNYRDLDIG 488
           FPHR G ++K+ +   W++  + +E+RH+ WIR++Y YM  +VSK+PR AY NYRDLD+G
Sbjct: 433 FPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLG 492

Query: 489 TNNKYGKTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPT 534
           TN   G+T    AR WG KY+  NF+RLV +K + DP +FFR+EQSVPT
Sbjct: 493 TNE--GET---DAREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of HG10021448 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 498.0 bits (1281), Expect = 9.3e-141
Identity = 255/540 (47.22%), Postives = 359/540 (66.48%), Query Frame = 0

Query: 5   FLSTFSLTFIVLSIS----PSWVVSTKKHEAFLQCLLNNSPSTYSISNLIYTPINSSFYS 64
           FL +  + F++L++S    PS       HE FL+CL +        S +I+T  + S++S
Sbjct: 6   FLLSLFIYFLLLNLSLSHFPSISAQRTNHENFLKCLSHR--INEDDSRIIHTSKDPSYFS 65

Query: 65  VLNFSIQNHRFSTKETPKPLAIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYV 124
           +LN SIQN RF   ETPKP++IITP   + +Q+ + C++ HG+ IRTRSGGHD+EGLSY+
Sbjct: 66  ILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYM 125

Query: 125 S-DVSFIVVDLINLRSIAVDVKNENAWVQSGTTLGELYYRIAEKSRTLAFPAGSCPTVGV 184
           +    F+V+DL NLRSI +DV N   WVQSG T+GELYY I + S++LAFPAG  PTVG+
Sbjct: 126 AKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGI 185

Query: 185 GGHLSGGGFGWLMRKYGLAADNVIDASFVDANGKVYDRESMGEDLFWAIRGGGGGSFGIV 244
           GG   GGG+G LMRKYGL+ADNVIDA  VDANG   DR+ MGED FWAIRGGGG SF +V
Sbjct: 186 GGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVV 245

Query: 245 VAWKVKLVRVPVTVTICGINRSLEKEDTIKLIYRWQYVTNKLDENLLLGISLNGGNSTQE 304
           ++WK++L+ VP  VT+  + ++ EKE  + +I +WQY+ +K+  +L +   L        
Sbjct: 246 LSWKIRLLDVPSVVTVFKVVKTSEKE-AVSIINKWQYIADKVPNDLFIRAMLQ------- 305

Query: 305 GGKTNPTALFSSFFLGKVNELMPILKTNFPELGLAKEDCSEMSWIKSTLIMAGFPKQEAF 364
             +T   A F   +LG V++L+ ++K  FPELGL   +C EMSWI+S L    F K E+ 
Sbjct: 306 -KETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVL---WFIKGESM 365

Query: 365 EVLLDRTPLFGLSTKIKSDYVKKPISEAAFKTLLKRLKAQDIEVAQIMFIPYGGRMSEIF 424
           E+L  R      S K K D++++PI + A + L +R +A +  +A+I+  P+GG+MSEI 
Sbjct: 366 EILAKRKRT-SRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIA 425

Query: 425 ESQTPFPHRAGNIYKLGYYVKWKEQSIDAEK----RHLNWIRDIYDYMTPFVSKSPRAAY 484
           +++ PFPHR GN+Y++ Y   W E+  D  K    ++L W+  +Y++MTP+VSKSPR AY
Sbjct: 426 DNEIPFPHREGNLYEIQYLAYWSEEE-DKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAY 485

Query: 485 NNYRDLDIGTNNKYG-KTIYSHARVWGLKYFGKNFDRLVHVKTKIDPLDFFRNEQSVPTL 535
            N+RD+D+G       KT Y  A+VWG+KYF  NFDRLV VKT +DP+DFF +EQS+P +
Sbjct: 486 VNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529