HG10021412 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021412 vs. NCBI nr
Match: XP_038894315.1 (cation-chloride cotransporter 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1907.5 bits (4940), Expect = 0.0e+00
Identity = 968/982 (98.57%), Postives = 975/982 (99.29%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPM HPSLKKVK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMSHPSLKKVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE
Sbjct: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL
Sbjct: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAGIFRETVTK+NGTTV EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGIFRETVTKVNGTTV-EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS++CIFLGIFLARKNDPA G+TGLSLESFKENWSSDYQMTNSAGIPDPF
Sbjct: 301 VAPAFLIPVLFSVLCIFLGIFLARKNDPAEGITGLSLESFKENWSSDYQMTNSAGIPDPF 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATR+KLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATRDKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIPATFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEG Q DESMEAFTAAQGRIASYLSEMK TAESRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGTQQDESMEAFTAAQGRIASYLSEMKTTAESRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 981

BLAST of HG10021412 vs. NCBI nr
Match: XP_008445134.1 (PREDICTED: cation-chloride cotransporter 1 isoform X1 [Cucumis melo])

HSP 1 Score: 1900.9 bits (4923), Expect = 0.0e+00
Identity = 965/982 (98.27%), Postives = 973/982 (99.08%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPP+RHPSLKKVK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPIRHPSLKKVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           VGSQTGTEK+GNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE
Sbjct: 61  VGSQTGTEKDGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           NVS+TQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL
Sbjct: 121 NVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAGIFRETVTK+NGTTV EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGIFRETVTKVNGTTV-EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS++CIFLG+FLA KNDP  GVTGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL EIPATFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLTEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEGAQ DESMEAFTAAQGRIASYLSEMKATAESRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGAQQDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 981

BLAST of HG10021412 vs. NCBI nr
Match: XP_011659141.1 (cation-chloride cotransporter 1 isoform X1 [Cucumis sativus] >KGN65931.1 hypothetical protein Csa_023216 [Cucumis sativus])

HSP 1 Score: 1894.4 bits (4906), Expect = 0.0e+00
Identity = 959/982 (97.66%), Postives = 971/982 (98.88%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSS+PP+RHPSLKKVK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSVPPIRHPSLKKVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           VGSQTGTEKEGNSPTRI+VNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE
Sbjct: 61  VGSQTGTEKEGNSPTRIDVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           NVS+TQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL
Sbjct: 121 NVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAGIFRETVTK+NGTTV EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGIFRETVTKVNGTTV-EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS++CIFLG+FLA KNDP  GVTGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTT +YLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTVMYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATR+KLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATRKKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIPATFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEG Q DESMEAFTAAQGRIASYLSEMK TAESRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGGQQDESMEAFTAAQGRIASYLSEMKETAESRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 981

BLAST of HG10021412 vs. NCBI nr
Match: XP_023001386.1 (cation-chloride cotransporter 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1864.7 bits (4829), Expect = 0.0e+00
Identity = 943/982 (96.03%), Postives = 966/982 (98.37%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPG+SSSSSS+PP+   SLK VK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGASSSSSSVPPI---SLKNVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           V SQTGTE +GNSPTRIEV+GPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP+SPRDGE
Sbjct: 61  VDSQTGTETDGNSPTRIEVDGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPTSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           N+SITQGLPKT EVKSGTLMGVFIPCLQNILGIIY+IRF+WIVGMAGIGESLVLV+FCGL
Sbjct: 121 NISITQGLPKTTEVKSGTLMGVFIPCLQNILGIIYFIRFTWIVGMAGIGESLVLVSFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAG+FRETVTK+NGTTVA PIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGLFRETVTKVNGTTVAAPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS+V IFLGIF+ARKNDP+ G+TGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVVSIFLGIFVARKNDPSDGITGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATREKLLTDRLLTATVAWPFPA+IYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATREKLLTDRLLTATVAWPFPALIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASL+YYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLLYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIP TFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPTTFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKE+FESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKETFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYL EMKA A+SRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLGEMKAAAKSRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQV KFLNTTLKLNSTILRYSRMAAVVLVSLPPPPV+HPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVGKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVDHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 979

BLAST of HG10021412 vs. NCBI nr
Match: XP_022927331.1 (cation-chloride cotransporter 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1860.9 bits (4819), Expect = 0.0e+00
Identity = 940/982 (95.72%), Postives = 966/982 (98.37%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN +IESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPG+SSSSSS+PP+   SLK VK
Sbjct: 1   MDNGEIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGASSSSSSVPPI---SLKNVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           V SQTGTE +GNSPTRIEV+GPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP+SPRDGE
Sbjct: 61  VDSQTGTEADGNSPTRIEVDGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPTSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           N+SITQGLPKT +VKSGTLMGVFIPCLQNILGIIY+IRF+WIVGMAGIGESLVLV+FCGL
Sbjct: 121 NISITQGLPKTTDVKSGTLMGVFIPCLQNILGIIYFIRFTWIVGMAGIGESLVLVSFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAG+FRETVTK+NGTTVA PIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGLFRETVTKVNGTTVAAPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS+V IFLGIF+ARKNDP+ G+TGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVVSIFLGIFVARKNDPSDGITGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATREKLLTDRLLTATVAWPFPA+IYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATREKLLTDRLLTATVAWPFPALIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASL+YYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLLYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVEDAK+ACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDAKSACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIP TFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPTTFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKE+FESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKETFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYL EMKA A+SRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLGEMKAAAKSRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQV KFLNTTLKLNSTILRYSRMAAVVLVSLPPPPV+HPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVGKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVDHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 979

BLAST of HG10021412 vs. ExPASy Swiss-Prot
Match: Q2UVJ5 (Cation-chloride cotransporter 1 OS=Arabidopsis thaliana OX=3702 GN=CCC1 PE=1 SV=1)

HSP 1 Score: 1596.6 bits (4133), Expect = 0.0e+00
Identity = 807/989 (81.60%), Postives = 888/989 (89.79%), Query Frame = 0

Query: 1   MDNADIE----SGEEEFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRH 60
           MD+ DIE    +GEEEF  G R  G KYRPV AHDRAV+EMSS+DPGSSSS         
Sbjct: 1   MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSS--------- 60

Query: 61  PSLKKVKVGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP 120
            +LK +KV +  G    G       VNG Q+ESKLE FGFDSLVNILGLKSM GEQIQAP
Sbjct: 61  -TLKNIKVVA-PGDVGAGVRGPEDGVNGHQKESKLELFGFDSLVNILGLKSMTGEQIQAP 120

Query: 121 SSPRDGENVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLV 180
           SSPRDGE++SITQG PK   +K GT+MGVF+PCLQNILGIIYYIRF+WIVGMAGIG+ LV
Sbjct: 121 SSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLV 180

Query: 181 LVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGAL 240
           LV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPEVG+SIGLCFFLGNAVAGAL
Sbjct: 181 LVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGAL 240

Query: 241 YVLGAVETFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFG 300
           YVLGAVETFL A PAAGIFRET+TK+NGT V+E IQSP+SHDLQVYGI+VTILLCFIVFG
Sbjct: 241 YVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSHDLQVYGIVVTILLCFIVFG 300

Query: 301 GVKMINRVAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNS 360
           GVKMINRVAPAFL+PVL S+ CIF+GIFLA+ +DP  G+TGL L+SFK+NW S YQMTN 
Sbjct: 301 GVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITGLRLKSFKDNWGSAYQMTND 360

Query: 361 AGIPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALY 420
           AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDTQ+SIP+GTLAATLTTT+LY
Sbjct: 361 AGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDTQKSIPVGTLAATLTTTSLY 420

Query: 421 LVSVLLFGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAI 480
           L+SVL FGA+ATR+KLLTDRLLTAT+AWPFPAI++VGIILSTLGAALQSLTGAPRLLAAI
Sbjct: 421 LISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILSTLGAALQSLTGAPRLLAAI 480

Query: 481 ANDDILPILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNL 540
           ANDDILPILNYFKVAD +EP+ ATLFTAF+CIGCV+IGNLDLITPTVTMF+LLCY+GVNL
Sbjct: 481 ANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLDLITPTVTMFYLLCYSGVNL 540

Query: 541 SCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCL 600
           SCFLLDLLDAPSWRPRW++HHWSLS +GASLC+VIMFLISWSFT+V++ALASLIY YV L
Sbjct: 541 SCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISWSFTVVAIALASLIYKYVGL 600

Query: 601 RGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKL 660
           +GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL+FCRPWG+LPENVPCHPKL
Sbjct: 601 KGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPLVFCRPWGQLPENVPCHPKL 660

Query: 661 ADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTM 720
           ADFANCMKKKGRGMSIFVSILDGDY+E  E+AK ACKQLATYI+YKRCEGVAEIVVAP M
Sbjct: 661 ADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLATYIEYKRCEGVAEIVVAPNM 720

Query: 721 SEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKG 780
           +EGFRGI+QTMGLGNLKPNIVVMRYPEIWRRENL EIP+TFVGIINDCI ANKAVVI+KG
Sbjct: 721 TEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTFVGIINDCITANKAVVIIKG 780

Query: 781 LDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGL 840
           LDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQ+FCIAEEDSDAE L
Sbjct: 781 LDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQLFCIAEEDSDAEAL 840

Query: 841 KADVKKFLYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAE 900
           KADVKKFLYDLRM AEVIV+T+KSWD + EG   ++S+EAF AAQ RI+ YL E+K    
Sbjct: 841 KADVKKFLYDLRMHAEVIVVTMKSWDIRSEGNSQEDSLEAFDAAQRRISDYLGEIKRQGS 900

Query: 901 SRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFY 960
           +    L+A+GKP+ VNEQQVEKFL T LKLNSTIL YSRMAAVVLVSLPPPP+NHPAYFY
Sbjct: 901 N---PLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSRMAAVVLVSLPPPPLNHPAYFY 960

Query: 961 MEYLDLLVENVPRLLIVRGYRRDVVTLFT 983
           MEY+DLLVENVPR+LIVRGY RDVVTLFT
Sbjct: 961 MEYMDLLVENVPRMLIVRGYHRDVVTLFT 975

BLAST of HG10021412 vs. ExPASy Swiss-Prot
Match: Q6Z0E2 (Cation-chloride cotransporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CCC1 PE=2 SV=1)

HSP 1 Score: 1546.9 bits (4004), Expect = 0.0e+00
Identity = 783/1002 (78.14%), Postives = 877/1002 (87.52%), Query Frame = 0

Query: 1   MDNADIESGEEEF----HGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSS-----SSIPPM 60
           M+N +IE   ++         GR+YRPV + DRAV++M+SM+PGSSSS+     S I P 
Sbjct: 1   MENGEIEGAADDGVPVPAPPNGRRYRPVGSSDRAVIQMTSMEPGSSSSTAVAAVSGITPQ 60

Query: 61  RHPSLKKVKVGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQ 120
              +L       +  T  +G             +SKLE FGFDSLVNILGLKSM GEQIQ
Sbjct: 61  PPRNLTVDPSMQEDHTVSQG-------------DSKLELFGFDSLVNILGLKSMTGEQIQ 120

Query: 121 APSSPRDGENVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGES 180
           APSSPRDGE+V+IT G PK    K GT+MGVF+PCLQNILGIIYYIRF+WIVGMAG+ +S
Sbjct: 121 APSSPRDGEDVAITIGRPKETGPKFGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGVWQS 180

Query: 181 LVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAG 240
           LVLV+FCG CTFLT ISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAG
Sbjct: 181 LVLVSFCGACTFLTGISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAG 240

Query: 241 ALYVLGAVETFLNAVPAAGIFRETVTKINGTTV-------AEPIQSPSSHDLQVYGIIVT 300
           ++YVLGAVETFL+AVP+AG F+E+VT +N T V          I +PS HDLQVYG+IVT
Sbjct: 241 SMYVLGAVETFLDAVPSAGFFKESVTVVNNTLVNGTATASTATISTPSLHDLQVYGVIVT 300

Query: 301 ILLCFIVFGGVKMINRVAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENW 360
           ILLCFIVFGGVK+IN+VAPAFLIPVLFSL+CI+LG+F+A +++   G+TGLS+ +FK+NW
Sbjct: 301 ILLCFIVFGGVKIINKVAPAFLIPVLFSLLCIYLGVFIAPRHNAPKGITGLSITTFKDNW 360

Query: 361 SSDYQMTNSAGIPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLA 420
            S+YQ TN+AG+PDP G +YW+FNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTL+
Sbjct: 361 GSEYQRTNNAGVPDPNGSIYWDFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLS 420

Query: 421 ATLTTTALYLVSVLLFGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLT 480
           ATLTTTA+YL SVLLFGALATRE+LLTDRLLTATVAWP PA+IY+GIILSTLGAALQSLT
Sbjct: 421 ATLTTTAMYLFSVLLFGALATREELLTDRLLTATVAWPAPAVIYIGIILSTLGAALQSLT 480

Query: 481 GAPRLLAAIANDDILPILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFF 540
           GAPRLLAAIANDDILP+LNYFKV++G EP+ ATLFTAF+CI CV+IGNLDLITPT+TMFF
Sbjct: 481 GAPRLLAAIANDDILPVLNYFKVSEGAEPHSATLFTAFICICCVVIGNLDLITPTITMFF 540

Query: 541 LLCYAGVNLSCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALA 600
           LLCYAGVNLSCFLLDLLDAPSWRPRW+FHHWSLSL+GA LCVVIMFLISWSFT+VSLALA
Sbjct: 541 LLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLVGALLCVVIMFLISWSFTVVSLALA 600

Query: 601 SLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLP 660
           SLIYYYV L+GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPLIFCRPWGKLP
Sbjct: 601 SLIYYYVSLKGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPLIFCRPWGKLP 660

Query: 661 ENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGV 720
           ENVPCHPKLADFANCMKKKGRGMSIFVSI+DGDYHE  EDAK AC+QL TYI+YKRCEGV
Sbjct: 661 ENVPCHPKLADFANCMKKKGRGMSIFVSIIDGDYHELAEDAKTACRQLDTYIEYKRCEGV 720

Query: 721 AEIVVAPTMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDA 780
           AEI+VAP+MSEGFR IVQTMGLGNLKPNI+VMRYPEIWRRENL++IP+TFV IINDCI A
Sbjct: 721 AEIIVAPSMSEGFRSIVQTMGLGNLKPNIIVMRYPEIWRRENLIQIPSTFVSIINDCIIA 780

Query: 781 NKAVVIVKGLDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIA 840
           NKAVVIVKGLDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKE+FESCKIQVFCIA
Sbjct: 781 NKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKETFESCKIQVFCIA 840

Query: 841 EEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDTQVE----GAQPDESMEAFTAAQGR 900
           EED+DAE LKADVKKFLYDLRM AEVIV+T+KSW+  +E    GA  D+S EA+T+AQ R
Sbjct: 841 EEDTDAEELKADVKKFLYDLRMHAEVIVVTMKSWEPHMESSSSGAPQDDSQEAYTSAQRR 900

Query: 901 IASYLSEMKATAESRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVS 960
           I++YLSEMK TA+  G  LM DGK V VNEQ++EKFL T  KLNSTILRYSRMAAVVLVS
Sbjct: 901 ISTYLSEMKETAQREGHPLMEDGKQVVVNEQKIEKFLYTMFKLNSTILRYSRMAAVVLVS 960

Query: 961 LPPPPVNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLFT 983
           LPPPP+NHPAYFYMEY+DLLVENVPR+LIVRGYRRDVVT FT
Sbjct: 961 LPPPPLNHPAYFYMEYMDLLVENVPRMLIVRGYRRDVVTFFT 989

BLAST of HG10021412 vs. ExPASy Swiss-Prot
Match: Q657W3 (Cation-chloride cotransporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CCC2 PE=2 SV=2)

HSP 1 Score: 1467.2 bits (3797), Expect = 0.0e+00
Identity = 735/975 (75.38%), Postives = 842/975 (86.36%), Query Frame = 0

Query: 19  RKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVKVGSQTGTEKEGN--SPTR 78
           ++YR VE+HDRAV++M+ M+ GSS+ +S+    R+     +K G+   T+   +  S   
Sbjct: 25  QRYRTVESHDRAVVQMAPMEFGSSADASASAGPRY-----IKPGTNLRTDARMHMASSNG 84

Query: 79  IEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSITQGLPKTNEVKS 138
              NG Q +SKLE FGFDSLVNILGLK M+GEQ QA +S RDGEN  I  G PK  E K 
Sbjct: 85  RSSNGSQGDSKLELFGFDSLVNILGLKRMVGEQAQASASTRDGENAGIAIGHPKETETKL 144

Query: 139 GTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNG 198
            T+MGVF+PCLQNILGIIYYIRF+WIVGM G+ +SLVLVAFCG CTFLT+ISLSAIATNG
Sbjct: 145 DTMMGVFVPCLQNILGIIYYIRFTWIVGMGGVWQSLVLVAFCGSCTFLTTISLSAIATNG 204

Query: 199 AMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLNAVPAAGIFRETV 258
           AMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGA+YVLGAVETFL+AVP+A  F+E+V
Sbjct: 205 AMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGAMYVLGAVETFLDAVPSAEFFQESV 264

Query: 259 TKINGTTV-------AEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINRVAPAFLIPV 318
           T +  T V       A  I +P+ HDLQVYGIIVTILLCFIVFGGVK+IN+VAPAFLIPV
Sbjct: 265 TVVTNTFVNGTAAGNATTISTPNLHDLQVYGIIVTILLCFIVFGGVKIINKVAPAFLIPV 324

Query: 319 LFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPFGKVYWNFNA 378
           LFS++CI++G+F+A + + +  +TGLS+ + K+NWSSDYQ TN+AG+PDP G +YW+FNA
Sbjct: 325 LFSILCIYIGVFIAPRPNASKWITGLSITTLKDNWSSDYQRTNNAGVPDPNGSIYWDFNA 384

Query: 379 LVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLFGALATREKL 438
           L+GL+FPAVTGIMAGSNRSASLKDTQRSIPIGTL AT++TT +YL+SV LFGAL+TRE L
Sbjct: 385 LLGLYFPAVTGIMAGSNRSASLKDTQRSIPIGTLHATISTTMMYLLSVFLFGALSTREGL 444

Query: 439 LTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILPILNYFKVAD 498
           LTDRLL A VAWP PA++Y GIILSTLGAALQSLTGAPRLLAAIANDDILP+LNYFK  +
Sbjct: 445 LTDRLLCAAVAWPSPAVVYAGIILSTLGAALQSLTGAPRLLAAIANDDILPVLNYFKAYE 504

Query: 499 GNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPR 558
           G+EP+ ATLFT+F+CI CVIIGNLD+ITPT+TMFFLLCYAGVNLSCFLLDLLDAPSWRPR
Sbjct: 505 GSEPHVATLFTSFICISCVIIGNLDVITPTITMFFLLCYAGVNLSCFLLDLLDAPSWRPR 564

Query: 559 WRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYF 618
           W+ HHWSLSL+GA LC+VIMF+ISW+FT+VSLALASLIYYYV L+GKAGDWGDGFKSAYF
Sbjct: 565 WKLHHWSLSLIGALLCIVIMFMISWTFTVVSLALASLIYYYVSLKGKAGDWGDGFKSAYF 624

Query: 619 QLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSI 678
           QLALRSLRS+GA+QVHPKNWYPIPLIFCRPWGKLPE+VPCHPKLADFANCMKKKGRGMSI
Sbjct: 625 QLALRSLRSMGANQVHPKNWYPIPLIFCRPWGKLPEDVPCHPKLADFANCMKKKGRGMSI 684

Query: 679 FVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGIVQTMGLGNL 738
           FVSI+DGDYHE  EDAK AC+QL+ YIDY+RCEGVAEI+VAP+ S GFR IVQTMGLGNL
Sbjct: 685 FVSIIDGDYHESAEDAKTACRQLSAYIDYRRCEGVAEIIVAPSTSIGFRSIVQTMGLGNL 744

Query: 739 KPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNEFQRQYGTID 798
           KPNIVVMRYPEIWRRENL +IP+TFV IINDCI ANKAVVIVKGLDEWPNE+QRQYGTID
Sbjct: 745 KPNIVVMRYPEIWRRENLTQIPSTFVSIINDCITANKAVVIVKGLDEWPNEYQRQYGTID 804

Query: 799 LYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKFLYDLRMQAE 858
           LYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEED++AE LKADVKKFLYDLRMQA+
Sbjct: 805 LYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDTEAEELKADVKKFLYDLRMQAD 864

Query: 859 VIVITIKSW--DTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLMADGKPVS 918
           VIV+T+KSW  D    G    +  E + +AQ RI +Y+S++K  AE     LM  GK V 
Sbjct: 865 VIVVTVKSWEADPDRSGGSKKDDPEVYRSAQSRIRTYISQLKEAAERERRPLMEGGKQVV 924

Query: 919 VNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLLVENVPRL 978
           V+EQ+VEKFL T LKLN+TILR+SRMA VVLVSLPPPP+NH AY YMEY+DLLVEN+PR+
Sbjct: 925 VDEQKVEKFLYTMLKLNATILRHSRMAVVVLVSLPPPPLNHLAYCYMEYMDLLVENIPRI 984

Query: 979 LIVRGYRRDVVTLFT 983
           LIVRGYRRDVVTLFT
Sbjct: 985 LIVRGYRRDVVTLFT 994

BLAST of HG10021412 vs. ExPASy Swiss-Prot
Match: Q9Y666 (Solute carrier family 12 member 7 OS=Homo sapiens OX=9606 GN=SLC12A7 PE=1 SV=3)

HSP 1 Score: 540.4 bits (1391), Expect = 4.2e-152
Identity = 340/979 (34.73%), Postives = 534/979 (54.55%), Query Frame = 0

Query: 135  KSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIAT 194
            + GT +GV++PCLQNILG+I ++R +WIVG+AG+ ES ++VA C  CT LT+IS+SAIAT
Sbjct: 117  RMGTFIGVYLPCLQNILGVILFLRLTWIVGVAGVLESFLIVAMCCTCTMLTAISMSAIAT 176

Query: 195  NGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLNAV-PAAGIFR 254
            NG +  GG YY+I R+LGPE G ++GLCF+LG   AGA+Y+LG +E FL  + P A IF+
Sbjct: 177  NGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYISPGAAIFQ 236

Query: 255  ETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINRVAPAFLIPVLFSL 314
                   G   A        H+++VYG    +L+  +VF GVK +N++A  FL  V+ S+
Sbjct: 237  AEAA--GGEAAA------MLHNMRVYGTCTLVLMALVVFVGVKYVNKLALVFLACVVLSI 296

Query: 315  VCIFLGIF------------------LARK---------------------------NDP 374
            + I+ G+                   L+R+                           + P
Sbjct: 297  LAIYAGVIKSAFDPPDIPVCLLGNRTLSRRSFDACVKAYGIHNNSATSALWGLFCNGSQP 356

Query: 375  AA---------------GVTGLSLESFKENWSSDY------------------QMTNSAG 434
            +A               G+ G +   F EN  S Y                  + + ++ 
Sbjct: 357  SAACDEYFIQNNVTEIQGIPGAASGVFLENLWSTYAHAGAFVEKKGVPSVPVAEESRASA 416

Query: 435  IPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLV 494
            +P     +  +F  LVG++FP+VTGIMAGSNRS  LKD Q+SIP GT+ A +TT+ +YL 
Sbjct: 417  LPYVLTDIAASFTLLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLS 476

Query: 495  SVLLFGA----LATREKL---LTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPR 554
             ++LFGA    +  R+K    L   L+   +AWP P +I +G   ST GA LQSLTGAPR
Sbjct: 477  CIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPR 536

Query: 555  LLAAIANDDILPILNYFKVADGN-EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLC 614
            LL AIA D I+P L  F     N EP +A L T  +C   ++I +LD + P ++MFFL+C
Sbjct: 537  LLQAIARDGIVPFLQVFGHGKANGEPTWALLLTVLICETGILIASLDSVAPILSMFFLMC 596

Query: 615  YAGVNLSCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLI 674
            Y  VNL+C +  LL  P+WRPR++F+HW+LS LG SLC+ +MF+ SW + + ++ +A  I
Sbjct: 597  YLFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALSAMLIAGCI 656

Query: 675  YYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENV 734
            Y Y+  RG   +WGDG +      A  +L  +     H KNW P  L+         E  
Sbjct: 657  YKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVML---NLDAEQA 716

Query: 735  PCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEI 794
              HP+L  F + + K G+G++I  S+L+G Y ++  +A+ A + + + +  ++ +G  ++
Sbjct: 717  VKHPRLLSFTSQL-KAGKGLTIVGSVLEGTYLDKHMEAQRAEENIRSLMSTEKTKGFCQL 776

Query: 795  VVAPTMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKA 854
            VV+ ++ +G   ++Q+ GLG LK N V+M +P  W++E+       FV  + D   A++A
Sbjct: 777  VVSSSLRDGMSHLIQSAGLGGLKHNTVLMAWPASWKQEDNPFSWKNFVDTVRDTTAAHQA 836

Query: 855  VVIVKGLDEWPNEFQR-QYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEE 914
            +++ K +D +P   +R   G ID++WIV DGG+++LL  LL   + +  C++++F +A+ 
Sbjct: 837  LLVAKNVDSFPQNQERFGGGHIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQV 896

Query: 915  DSDAEGLKADVKKFLYDLRMQAEVIVITIKSWD-------------------TQVEGAQP 974
            D ++  +K D++ FLY LR+ AEV V+ +   D                    Q++ ++ 
Sbjct: 897  DDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYERTLMMEQRSQMLKQMQLSKN 956

Query: 975  DESMEA----------FTAAQGR---------IASYLSEMKATAE---SRGTTLMADGKP 983
            ++  EA           TAA  R         +    +  K  AE   SR T+L      
Sbjct: 957  EQEREAQLIHDRNTASHTAAAARTQAPPTPDKVQMTWTREKLIAEKYRSRDTSLSGFKDL 1016

BLAST of HG10021412 vs. ExPASy Swiss-Prot
Match: Q9WVL3 (Solute carrier family 12 member 7 OS=Mus musculus OX=10090 GN=Slc12a7 PE=1 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 8.7e-150
Identity = 334/987 (33.84%), Postives = 526/987 (53.29%), Query Frame = 0

Query: 130  KTNEVKS---GTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTS 189
            +  EVK+   GT +GV++PCLQNILG+I ++R +WIVG AG+ ES ++VA C  CT LT+
Sbjct: 109  RRREVKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGAAGVMESFLIVAMCCTCTMLTA 168

Query: 190  ISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLNAV 249
            IS+SAIATNG +  GG YY+I R+LGPE G ++GLCF+LG   AGA+Y+LG +E FL  +
Sbjct: 169  ISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYI 228

Query: 250  -PAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINRVAPAF 309
             P+A IF+           A+   +   ++++VYG     L+  +VF GVK +N++A  F
Sbjct: 229  SPSAAIFQ--------AETADGEAAALLNNMRVYGSCALALMAVVVFVGVKYVNKLALVF 288

Query: 310  LIPVLFSLVCIFLGIF------------------LARKN-DPAA---------------- 369
            L  V+ S++ I+ G+                   LA +N D  A                
Sbjct: 289  LACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKMQVVSNGTVTTALWR 348

Query: 370  -------------------------GVTGLSLESFKENWSSDY----------------- 429
                                     G+ G++   F +N  S Y                 
Sbjct: 349  LFCNGSSLGATCDEYFAQNNVTEIQGIPGVASGVFLDNLWSTYSDKGAFVEKKGVSSVPV 408

Query: 430  -QMTNSAGIPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATL 489
             + +   G+P     +   F  LVG++FP+VTGIMAGSNRS  LKD Q+SIP GT+ A +
Sbjct: 409  SEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIV 468

Query: 490  TTTALYLVSVLLFGA----LATREKL---LTDRLLTATVAWPFPAIIYVGIILSTLGAAL 549
            TT+ +YL  ++LFGA    +  R+K    L   L+   +AWP P +I +G   ST GA L
Sbjct: 469  TTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGL 528

Query: 550  QSLTGAPRLLAAIANDDILPILNYFKVADGN-EPYFATLFTAFLCIGCVIIGNLDLITPT 609
            QSLTGAPRLL AIA D I+P L  F     N EP +A L TA +C   ++I +LD + P 
Sbjct: 529  QSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTALICETGILIASLDSVAPI 588

Query: 610  VTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIV 669
            ++MFFL+CY  VNL+C +  LL  P+WRPR++F+HW+LS LG SLC+ +MF+ SW + + 
Sbjct: 589  LSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALF 648

Query: 670  SLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRP 729
            ++ +A  IY Y+  RG   +WGDG +      A  +L  +     H KNW P  L+    
Sbjct: 649  AMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVML-- 708

Query: 730  WGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYK 789
                 E    HP+L  F + + K G+G++I  S+L+G Y ++  +A+ A + + + +  +
Sbjct: 709  -NLDSEQCVKHPRLLSFTSQL-KAGKGLTIVGSVLEGTYLDKHVEAQRAEENIRSLMSAE 768

Query: 790  RCEGVAEIVVAPTMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIIN 849
            + +G  ++VV+  + +G   ++Q+ GLG +K N V+M +PE W+  +       FV  + 
Sbjct: 769  KTKGFCQLVVSSNLRDGASHLIQSAGLGGMKHNTVLMAWPEAWKEADNPFSWKNFVDTVR 828

Query: 850  DCIDANKAVVIVKGLDEWPNEFQR-QYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKI 909
            D   A++A+++ K +D +P   +R   G ID++WIV DGG+++LL  LL   + +  C++
Sbjct: 829  DTTAAHQALLVAKNIDLFPQNQERFSDGNIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRM 888

Query: 910  QVFCIAEEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDTQV----------EGAQPD 969
            ++F +A+ D ++  +K D++ FLY LR+ AEV V+ +   D             + +Q  
Sbjct: 889  RIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYEKTLMMEQRSQML 948

Query: 970  ESMEAFTAAQGRIASYLSEMKATAESRGTT-LMADGKPVSVN------------------ 983
            + M+     + R A  + +    + +  T    A   P  V                   
Sbjct: 949  KQMQLSKNEREREAQLIHDRNTASHTTATARTQAPPTPDKVQMTWTKEKLIAEKHRNKDT 1008

BLAST of HG10021412 vs. ExPASy TrEMBL
Match: A0A1S3BBY4 (cation-chloride cotransporter 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488259 PE=3 SV=1)

HSP 1 Score: 1900.9 bits (4923), Expect = 0.0e+00
Identity = 965/982 (98.27%), Postives = 973/982 (99.08%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPP+RHPSLKKVK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPIRHPSLKKVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           VGSQTGTEK+GNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE
Sbjct: 61  VGSQTGTEKDGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           NVS+TQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL
Sbjct: 121 NVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAGIFRETVTK+NGTTV EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGIFRETVTKVNGTTV-EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS++CIFLG+FLA KNDP  GVTGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL EIPATFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLTEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEGAQ DESMEAFTAAQGRIASYLSEMKATAESRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGAQQDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 981

BLAST of HG10021412 vs. ExPASy TrEMBL
Match: A0A0A0M162 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538780 PE=3 SV=1)

HSP 1 Score: 1894.4 bits (4906), Expect = 0.0e+00
Identity = 959/982 (97.66%), Postives = 971/982 (98.88%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSS+PP+RHPSLKKVK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSVPPIRHPSLKKVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           VGSQTGTEKEGNSPTRI+VNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE
Sbjct: 61  VGSQTGTEKEGNSPTRIDVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           NVS+TQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL
Sbjct: 121 NVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAGIFRETVTK+NGTTV EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGIFRETVTKVNGTTV-EPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS++CIFLG+FLA KNDP  GVTGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTT +YLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTVMYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATR+KLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATRKKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIPATFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEG Q DESMEAFTAAQGRIASYLSEMK TAESRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGGQQDESMEAFTAAQGRIASYLSEMKETAESRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 981

BLAST of HG10021412 vs. ExPASy TrEMBL
Match: A0A6J1KIG9 (cation-chloride cotransporter 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495539 PE=3 SV=1)

HSP 1 Score: 1864.7 bits (4829), Expect = 0.0e+00
Identity = 943/982 (96.03%), Postives = 966/982 (98.37%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN DIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPG+SSSSSS+PP+   SLK VK
Sbjct: 1   MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGASSSSSSVPPI---SLKNVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           V SQTGTE +GNSPTRIEV+GPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP+SPRDGE
Sbjct: 61  VDSQTGTETDGNSPTRIEVDGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPTSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           N+SITQGLPKT EVKSGTLMGVFIPCLQNILGIIY+IRF+WIVGMAGIGESLVLV+FCGL
Sbjct: 121 NISITQGLPKTTEVKSGTLMGVFIPCLQNILGIIYFIRFTWIVGMAGIGESLVLVSFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAG+FRETVTK+NGTTVA PIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGLFRETVTKVNGTTVAAPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS+V IFLGIF+ARKNDP+ G+TGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVVSIFLGIFVARKNDPSDGITGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATREKLLTDRLLTATVAWPFPA+IYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATREKLLTDRLLTATVAWPFPALIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASL+YYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLLYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIP TFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPTTFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKE+FESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKETFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYL EMKA A+SRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLGEMKAAAKSRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQV KFLNTTLKLNSTILRYSRMAAVVLVSLPPPPV+HPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVGKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVDHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 979

BLAST of HG10021412 vs. ExPASy TrEMBL
Match: A0A6J1EHD5 (cation-chloride cotransporter 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434191 PE=3 SV=1)

HSP 1 Score: 1860.9 bits (4819), Expect = 0.0e+00
Identity = 940/982 (95.72%), Postives = 966/982 (98.37%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDN +IESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPG+SSSSSS+PP+   SLK VK
Sbjct: 1   MDNGEIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGASSSSSSVPPI---SLKNVK 60

Query: 61  VGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGE 120
           V SQTGTE +GNSPTRIEV+GPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP+SPRDGE
Sbjct: 61  VDSQTGTEADGNSPTRIEVDGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPTSPRDGE 120

Query: 121 NVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGL 180
           N+SITQGLPKT +VKSGTLMGVFIPCLQNILGIIY+IRF+WIVGMAGIGESLVLV+FCGL
Sbjct: 121 NISITQGLPKTTDVKSGTLMGVFIPCLQNILGIIYFIRFTWIVGMAGIGESLVLVSFCGL 180

Query: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240
           CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE
Sbjct: 181 CTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVE 240

Query: 241 TFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300
           TFLNAVPAAG+FRETVTK+NGTTVA PIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR
Sbjct: 241 TFLNAVPAAGLFRETVTKVNGTTVAAPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMINR 300

Query: 301 VAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDPF 360
           VAPAFLIPVLFS+V IFLGIF+ARKNDP+ G+TGLSLESFKENWSSDYQMTNSAGIPDP 
Sbjct: 301 VAPAFLIPVLFSVVSIFLGIFVARKNDPSDGITGLSLESFKENWSSDYQMTNSAGIPDPL 360

Query: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420
           GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF
Sbjct: 361 GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLLF 420

Query: 421 GALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480
           GALATREKLLTDRLLTATVAWPFPA+IYVGIILSTLGAALQSLTGAPRLLAAIANDDILP
Sbjct: 421 GALATREKLLTDRLLTATVAWPFPALIYVGIILSTLGAALQSLTGAPRLLAAIANDDILP 480

Query: 481 ILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540
           ILNYFKVADG+EPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL
Sbjct: 481 ILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDL 540

Query: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDW 600
           LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASL+YYYVCLRGKAGDW
Sbjct: 541 LDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLLYYYVCLRGKAGDW 600

Query: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660
           GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM
Sbjct: 601 GDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCM 660

Query: 661 KKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720
           KKKGRGMSIFVSILDGDYHERVEDAK+ACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI
Sbjct: 661 KKKGRGMSIFVSILDGDYHERVEDAKSACKQLATYIDYKRCEGVAEIVVAPTMSEGFRGI 720

Query: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPNE 780
           VQTMGLGNLKPNIVVMRYPEIWRRENL+EIP TFVGIINDCIDANKAVVIVKGLDEWPNE
Sbjct: 721 VQTMGLGNLKPNIVVMRYPEIWRRENLIEIPTTFVGIINDCIDANKAVVIVKGLDEWPNE 780

Query: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKKF 840
           FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKE+FESCKIQVFCIAEEDSDAEGLKADVKKF
Sbjct: 781 FQRQYGTIDLYWIVRDGGLMLLLSQLLLTKETFESCKIQVFCIAEEDSDAEGLKADVKKF 840

Query: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTLM 900
           LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYL EMKA A+SRGTTLM
Sbjct: 841 LYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLGEMKAAAKSRGTTLM 900

Query: 901 ADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDLL 960
           ADGKPVSVNEQQV KFLNTTLKLNSTILRYSRMAAVVLVSLPPPPV+HPAYFYMEYLDLL
Sbjct: 901 ADGKPVSVNEQQVGKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVDHPAYFYMEYLDLL 960

Query: 961 VENVPRLLIVRGYRRDVVTLFT 983
           VENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 VENVPRLLIVRGYRRDVVTLFT 979

BLAST of HG10021412 vs. ExPASy TrEMBL
Match: A0A6J1CH20 (cation-chloride cotransporter 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010778 PE=3 SV=1)

HSP 1 Score: 1845.9 bits (4780), Expect = 0.0e+00
Identity = 935/983 (95.12%), Postives = 963/983 (97.97%), Query Frame = 0

Query: 1   MDNADIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRHPSLKKVK 60
           MDNADIESGEE+FHG+RGRKYRPVEAHDRAVLEMSSMDPGSSSSS S+ PMRHPS+KKVK
Sbjct: 1   MDNADIESGEEDFHGKRGRKYRPVEAHDRAVLEMSSMDPGSSSSSPSV-PMRHPSMKKVK 60

Query: 61  VGSQTGT-EKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDG 120
           VGSQ G+ EKEGNSP+RIEVNGPQR+SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDG
Sbjct: 61  VGSQGGSDEKEGNSPSRIEVNGPQRDSKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDG 120

Query: 121 ENVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCG 180
           E+V ITQGLPKTNEVKSGT+MGVFIPCLQNILGIIYYIRF+WIVGMAGIGESLVLVAFCG
Sbjct: 121 ESVPITQGLPKTNEVKSGTMMGVFIPCLQNILGIIYYIRFTWIVGMAGIGESLVLVAFCG 180

Query: 181 LCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAV 240
           LCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAV
Sbjct: 181 LCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAV 240

Query: 241 ETFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFGGVKMIN 300
           ETFLNAVPAAGIFRE VTK+NGTTVA PIQSPSSHDLQVYGIIVTILLCFIVFGGVKMIN
Sbjct: 241 ETFLNAVPAAGIFREAVTKVNGTTVA-PIQSPSSHDLQVYGIIVTILLCFIVFGGVKMIN 300

Query: 301 RVAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNSAGIPDP 360
           RVAPAFLIPVLFSL+CIFLG+FLA KNDPA GVTGLSLESFKENWSSDYQMTNSAGIPDP
Sbjct: 301 RVAPAFLIPVLFSLLCIFLGVFLAGKNDPADGVTGLSLESFKENWSSDYQMTNSAGIPDP 360

Query: 361 FGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALYLVSVLL 420
            GKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAAT  TT+LYLVSVLL
Sbjct: 361 LGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATFVTTSLYLVSVLL 420

Query: 421 FGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDIL 480
           FGALATREKLLTDRLLTAT+AWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDIL
Sbjct: 421 FGALATREKLLTDRLLTATIAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAIANDDIL 480

Query: 481 PILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLD 540
           PILNYFKVADG+EPYFATLFTAFLC+GCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLD
Sbjct: 481 PILNYFKVADGSEPYFATLFTAFLCVGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLD 540

Query: 541 LLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGD 600
           LLDAPSWRPRW+FHHWSLSLLGASLCVVIMFLISWSFTIV+LALASLIYYYVCLRGKAGD
Sbjct: 541 LLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVALALASLIYYYVCLRGKAGD 600

Query: 601 WGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANC 660
           WGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANC
Sbjct: 601 WGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANC 660

Query: 661 MKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTMSEGFRG 720
           MKKKGRGMSIFVSILDGDYHERVED+K+ACKQLATYIDYKRCEGVAEIVVAP+MSEGFRG
Sbjct: 661 MKKKGRGMSIFVSILDGDYHERVEDSKSACKQLATYIDYKRCEGVAEIVVAPSMSEGFRG 720

Query: 721 IVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKGLDEWPN 780
           IVQTMGLGNLKPNIVVMRYPEIWRRENL EIPATFVGIINDCIDANKAVVIVKGLDEWPN
Sbjct: 721 IVQTMGLGNLKPNIVVMRYPEIWRRENLTEIPATFVGIINDCIDANKAVVIVKGLDEWPN 780

Query: 781 EFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGLKADVKK 840
           E+QRQYGTIDLYWIVRDGGLMLLLSQLLLTK SFESCKIQVFCIAEE+SDAEGLKADVKK
Sbjct: 781 EYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKASFESCKIQVFCIAEEESDAEGLKADVKK 840

Query: 841 FLYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAESRGTTL 900
           FLYDLRMQAEVIVITIKSW  Q +G QPDESMEAFTAAQGRIASYL EMKA AESRGTTL
Sbjct: 841 FLYDLRMQAEVIVITIKSWGVQGDGGQPDESMEAFTAAQGRIASYLGEMKAAAESRGTTL 900

Query: 901 MADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDL 960
           MADGKPVSVNE+QVEKFL+TTLKLN+TILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDL
Sbjct: 901 MADGKPVSVNEKQVEKFLDTTLKLNTTILRYSRMAAVVLVSLPPPPVNHPAYFYMEYLDL 960

Query: 961 LVENVPRLLIVRGYRRDVVTLFT 983
           LVENVPRLLIVRGYRRDVVTLFT
Sbjct: 961 LVENVPRLLIVRGYRRDVVTLFT 981

BLAST of HG10021412 vs. TAIR 10
Match: AT1G30450.1 (cation-chloride co-transporter 1 )

HSP 1 Score: 1596.6 bits (4133), Expect = 0.0e+00
Identity = 807/989 (81.60%), Postives = 888/989 (89.79%), Query Frame = 0

Query: 1   MDNADIE----SGEEEFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRH 60
           MD+ DIE    +GEEEF  G R  G KYRPV AHDRAV+EMSS+DPGSSSS         
Sbjct: 1   MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSS--------- 60

Query: 61  PSLKKVKVGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP 120
            +LK +KV +  G    G       VNG Q+ESKLE FGFDSLVNILGLKSM GEQIQAP
Sbjct: 61  -TLKNIKVVA-PGDVGAGVRGPEDGVNGHQKESKLELFGFDSLVNILGLKSMTGEQIQAP 120

Query: 121 SSPRDGENVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLV 180
           SSPRDGE++SITQG PK   +K GT+MGVF+PCLQNILGIIYYIRF+WIVGMAGIG+ LV
Sbjct: 121 SSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLV 180

Query: 181 LVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGAL 240
           LV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPEVG+SIGLCFFLGNAVAGAL
Sbjct: 181 LVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGAL 240

Query: 241 YVLGAVETFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFG 300
           YVLGAVETFL A PAAGIFRET+TK+NGT V+E IQSP+SHDLQVYGI+VTILLCFIVFG
Sbjct: 241 YVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSHDLQVYGIVVTILLCFIVFG 300

Query: 301 GVKMINRVAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNS 360
           GVKMINRVAPAFL+PVL S+ CIF+GIFLA+ +DP  G+TGL L+SFK+NW S YQMTN 
Sbjct: 301 GVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITGLRLKSFKDNWGSAYQMTND 360

Query: 361 AGIPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALY 420
           AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDTQ+SIP+GTLAATLTTT+LY
Sbjct: 361 AGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDTQKSIPVGTLAATLTTTSLY 420

Query: 421 LVSVLLFGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAI 480
           L+SVL FGA+ATR+KLLTDRLLTAT+AWPFPAI++VGIILSTLGAALQSLTGAPRLLAAI
Sbjct: 421 LISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILSTLGAALQSLTGAPRLLAAI 480

Query: 481 ANDDILPILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNL 540
           ANDDILPILNYFKVAD +EP+ ATLFTAF+CIGCV+IGNLDLITPTVTMF+LLCY+GVNL
Sbjct: 481 ANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLDLITPTVTMFYLLCYSGVNL 540

Query: 541 SCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCL 600
           SCFLLDLLDAPSWRPRW++HHWSLS +GASLC+VIMFLISWSFT+V++ALASLIY YV L
Sbjct: 541 SCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISWSFTVVAIALASLIYKYVGL 600

Query: 601 RGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKL 660
           +GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL+FCRPWG+LPENVPCHPKL
Sbjct: 601 KGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPLVFCRPWGQLPENVPCHPKL 660

Query: 661 ADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTM 720
           ADFANCMKKKGRGMSIFVSILDGDY+E  E+AK ACKQLATYI+YKRCEGVAEIVVAP M
Sbjct: 661 ADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLATYIEYKRCEGVAEIVVAPNM 720

Query: 721 SEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKG 780
           +EGFRGI+QTMGLGNLKPNIVVMRYPEIWRRENL EIP+TFVGIINDCI ANKAVVI+KG
Sbjct: 721 TEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTFVGIINDCITANKAVVIIKG 780

Query: 781 LDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGL 840
           LDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQ+FCIAEEDSDAE L
Sbjct: 781 LDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQLFCIAEEDSDAEAL 840

Query: 841 KADVKKFLYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAE 900
           KADVKKFLYDLRM AEVIV+T+KSWD + EG   ++S+EAF AAQ RI+ YL E+K    
Sbjct: 841 KADVKKFLYDLRMHAEVIVVTMKSWDIRSEGNSQEDSLEAFDAAQRRISDYLGEIKRQGS 900

Query: 901 SRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFY 960
           +    L+A+GKP+ VNEQQVEKFL T LKLNSTIL YSRMAAVVLVSLPPPP+NHPAYFY
Sbjct: 901 N---PLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSRMAAVVLVSLPPPPLNHPAYFY 960

Query: 961 MEYLDLLVENVPRLLIVRGYRRDVVTLFT 983
           MEY+DLLVENVPR+LIVRGY RDVVTLFT
Sbjct: 961 MEYMDLLVENVPRMLIVRGYHRDVVTLFT 975

BLAST of HG10021412 vs. TAIR 10
Match: AT1G30450.3 (cation-chloride co-transporter 1 )

HSP 1 Score: 1596.6 bits (4133), Expect = 0.0e+00
Identity = 807/989 (81.60%), Postives = 888/989 (89.79%), Query Frame = 0

Query: 1   MDNADIE----SGEEEFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRH 60
           MD+ DIE    +GEEEF  G R  G KYRPV AHDRAV+EMSS+DPGSSSS         
Sbjct: 1   MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSS--------- 60

Query: 61  PSLKKVKVGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP 120
            +LK +KV +  G    G       VNG Q+ESKLE FGFDSLVNILGLKSM GEQIQAP
Sbjct: 61  -TLKNIKVVA-PGDVGAGVRGPEDGVNGHQKESKLELFGFDSLVNILGLKSMTGEQIQAP 120

Query: 121 SSPRDGENVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLV 180
           SSPRDGE++SITQG PK   +K GT+MGVF+PCLQNILGIIYYIRF+WIVGMAGIG+ LV
Sbjct: 121 SSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLV 180

Query: 181 LVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGAL 240
           LV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPEVG+SIGLCFFLGNAVAGAL
Sbjct: 181 LVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGAL 240

Query: 241 YVLGAVETFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFG 300
           YVLGAVETFL A PAAGIFRET+TK+NGT V+E IQSP+SHDLQVYGI+VTILLCFIVFG
Sbjct: 241 YVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSHDLQVYGIVVTILLCFIVFG 300

Query: 301 GVKMINRVAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNS 360
           GVKMINRVAPAFL+PVL S+ CIF+GIFLA+ +DP  G+TGL L+SFK+NW S YQMTN 
Sbjct: 301 GVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITGLRLKSFKDNWGSAYQMTND 360

Query: 361 AGIPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALY 420
           AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDTQ+SIP+GTLAATLTTT+LY
Sbjct: 361 AGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDTQKSIPVGTLAATLTTTSLY 420

Query: 421 LVSVLLFGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAI 480
           L+SVL FGA+ATR+KLLTDRLLTAT+AWPFPAI++VGIILSTLGAALQSLTGAPRLLAAI
Sbjct: 421 LISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILSTLGAALQSLTGAPRLLAAI 480

Query: 481 ANDDILPILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNL 540
           ANDDILPILNYFKVAD +EP+ ATLFTAF+CIGCV+IGNLDLITPTVTMF+LLCY+GVNL
Sbjct: 481 ANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLDLITPTVTMFYLLCYSGVNL 540

Query: 541 SCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCL 600
           SCFLLDLLDAPSWRPRW++HHWSLS +GASLC+VIMFLISWSFT+V++ALASLIY YV L
Sbjct: 541 SCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISWSFTVVAIALASLIYKYVGL 600

Query: 601 RGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKL 660
           +GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL+FCRPWG+LPENVPCHPKL
Sbjct: 601 KGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPLVFCRPWGQLPENVPCHPKL 660

Query: 661 ADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTM 720
           ADFANCMKKKGRGMSIFVSILDGDY+E  E+AK ACKQLATYI+YKRCEGVAEIVVAP M
Sbjct: 661 ADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLATYIEYKRCEGVAEIVVAPNM 720

Query: 721 SEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKG 780
           +EGFRGI+QTMGLGNLKPNIVVMRYPEIWRRENL EIP+TFVGIINDCI ANKAVVI+KG
Sbjct: 721 TEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTFVGIINDCITANKAVVIIKG 780

Query: 781 LDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGL 840
           LDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQ+FCIAEEDSDAE L
Sbjct: 781 LDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQLFCIAEEDSDAEAL 840

Query: 841 KADVKKFLYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAE 900
           KADVKKFLYDLRM AEVIV+T+KSWD + EG   ++S+EAF AAQ RI+ YL E+K    
Sbjct: 841 KADVKKFLYDLRMHAEVIVVTMKSWDIRSEGNSQEDSLEAFDAAQRRISDYLGEIKRQGS 900

Query: 901 SRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFY 960
           +    L+A+GKP+ VNEQQVEKFL T LKLNSTIL YSRMAAVVLVSLPPPP+NHPAYFY
Sbjct: 901 N---PLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSRMAAVVLVSLPPPPLNHPAYFY 960

Query: 961 MEYLDLLVENVPRLLIVRGYRRDVVTLFT 983
           MEY+DLLVENVPR+LIVRGY RDVVTLFT
Sbjct: 961 MEYMDLLVENVPRMLIVRGYHRDVVTLFT 975

BLAST of HG10021412 vs. TAIR 10
Match: AT1G30450.2 (cation-chloride co-transporter 1 )

HSP 1 Score: 1596.6 bits (4133), Expect = 0.0e+00
Identity = 807/989 (81.60%), Postives = 888/989 (89.79%), Query Frame = 0

Query: 1   MDNADIE----SGEEEFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPMRH 60
           MD+ DIE    +GEEEF  G R  G KYRPV AHDRAV+EMSS+DPGSSSS         
Sbjct: 1   MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSS--------- 60

Query: 61  PSLKKVKVGSQTGTEKEGNSPTRIEVNGPQRESKLEFFGFDSLVNILGLKSMMGEQIQAP 120
            +LK +KV +  G    G       VNG Q+ESKLE FGFDSLVNILGLKSM GEQIQAP
Sbjct: 61  -TLKNIKVVA-PGDVGAGVRGPEDGVNGHQKESKLELFGFDSLVNILGLKSMTGEQIQAP 120

Query: 121 SSPRDGENVSITQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLV 180
           SSPRDGE++SITQG PK   +K GT+MGVF+PCLQNILGIIYYIRF+WIVGMAGIG+ LV
Sbjct: 121 SSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGIIYYIRFTWIVGMAGIGQGLV 180

Query: 181 LVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGAL 240
           LV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPEVG+SIGLCFFLGNAVAGAL
Sbjct: 181 LVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPEVGISIGLCFFLGNAVAGAL 240

Query: 241 YVLGAVETFLNAVPAAGIFRETVTKINGTTVAEPIQSPSSHDLQVYGIIVTILLCFIVFG 300
           YVLGAVETFL A PAAGIFRET+TK+NGT V+E IQSP+SHDLQVYGI+VTILLCFIVFG
Sbjct: 241 YVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSHDLQVYGIVVTILLCFIVFG 300

Query: 301 GVKMINRVAPAFLIPVLFSLVCIFLGIFLARKNDPAAGVTGLSLESFKENWSSDYQMTNS 360
           GVKMINRVAPAFL+PVL S+ CIF+GIFLA+ +DP  G+TGL L+SFK+NW S YQMTN 
Sbjct: 301 GVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITGLRLKSFKDNWGSAYQMTND 360

Query: 361 AGIPDPFGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGTLAATLTTTALY 420
           AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDTQ+SIP+GTLAATLTTT+LY
Sbjct: 361 AGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDTQKSIPVGTLAATLTTTSLY 420

Query: 421 LVSVLLFGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLAAI 480
           L+SVL FGA+ATR+KLLTDRLLTAT+AWPFPAI++VGIILSTLGAALQSLTGAPRLLAAI
Sbjct: 421 LISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILSTLGAALQSLTGAPRLLAAI 480

Query: 481 ANDDILPILNYFKVADGNEPYFATLFTAFLCIGCVIIGNLDLITPTVTMFFLLCYAGVNL 540
           ANDDILPILNYFKVAD +EP+ ATLFTAF+CIGCV+IGNLDLITPTVTMF+LLCY+GVNL
Sbjct: 481 ANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLDLITPTVTMFYLLCYSGVNL 540

Query: 541 SCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYVCL 600
           SCFLLDLLDAPSWRPRW++HHWSLS +GASLC+VIMFLISWSFT+V++ALASLIY YV L
Sbjct: 541 SCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISWSFTVVAIALASLIYKYVGL 600

Query: 601 RGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHPKL 660
           +GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL+FCRPWG+LPENVPCHPKL
Sbjct: 601 KGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPLVFCRPWGQLPENVPCHPKL 660

Query: 661 ADFANCMKKKGRGMSIFVSILDGDYHERVEDAKAACKQLATYIDYKRCEGVAEIVVAPTM 720
           ADFANCMKKKGRGMSIFVSILDGDY+E  E+AK ACKQLATYI+YKRCEGVAEIVVAP M
Sbjct: 661 ADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLATYIEYKRCEGVAEIVVAPNM 720

Query: 721 SEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLVEIPATFVGIINDCIDANKAVVIVKG 780
           +EGFRGI+QTMGLGNLKPNIVVMRYPEIWRRENL EIP+TFVGIINDCI ANKAVVI+KG
Sbjct: 721 TEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTFVGIINDCITANKAVVIIKG 780

Query: 781 LDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAEGL 840
           LDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQ+FCIAEEDSDAE L
Sbjct: 781 LDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQLFCIAEEDSDAEAL 840

Query: 841 KADVKKFLYDLRMQAEVIVITIKSWDTQVEGAQPDESMEAFTAAQGRIASYLSEMKATAE 900
           KADVKKFLYDLRM AEVIV+T+KSWD + EG   ++S+EAF AAQ RI+ YL E+K    
Sbjct: 841 KADVKKFLYDLRMHAEVIVVTMKSWDIRSEGNSQEDSLEAFDAAQRRISDYLGEIKRQGS 900

Query: 901 SRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLVSLPPPPVNHPAYFY 960
           +    L+A+GKP+ VNEQQVEKFL T LKLNSTIL YSRMAAVVLVSLPPPP+NHPAYFY
Sbjct: 901 N---PLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSRMAAVVLVSLPPPPLNHPAYFY 960

Query: 961 MEYLDLLVENVPRLLIVRGYRRDVVTLFT 983
           MEY+DLLVENVPR+LIVRGY RDVVTLFT
Sbjct: 961 MEYMDLLVENVPRMLIVRGYHRDVVTLFT 975