HG10021362 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021362 vs. NCBI nr
Match: XP_038894075.1 (glucan endo-1,3-beta-glucosidase 11-like [Benincasa hispida])

HSP 1 Score: 749.2 bits (1933), Expect = 1.9e-212
Identity = 389/406 (95.81%), Postives = 396/406 (97.54%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFHIPLLFLLSFSG FGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD
Sbjct: 1   MFFHIPLLFLLSFSG-FGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TNP+VLSAFANSKVEIIVTVENEMLAQLMD QQALQWVTARIKPYVPATRITGIAVGNEV
Sbjct: 61  TNPEVLSAFANSKVEIIVTVENEMLAQLMDAQQALQWVTARIKPYVPATRITGIAVGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTD+DMTLMETLVPAM+SIHTAL QLGLD +IKISTPSSLAVL+ESYPPSAGSFKPEITG
Sbjct: 121 FTDNDMTLMETLVPAMLSIHTALTQLGLD-AIKISTPSSLAVLQESYPPSAGSFKPEITG 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFLSTTKSPFWINAYPYFAYK DPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY
Sbjct: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKGDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAKMGFGGIEVRISETGWPSKGDP+ETGA+VENAANYNRNLLRRQMANEGT
Sbjct: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPDETGATVENAANYNRNLLRRQMANEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFS 360
           PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSS   SFS
Sbjct: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSS---SFS 360

Query: 361 PSSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRRPLYN 407
           PSSISLSSSA TN KGATMGYYQSLVYWMFVYLLTYQVFMRRPLYN
Sbjct: 361 PSSISLSSSASTNPKGATMGYYQSLVYWMFVYLLTYQVFMRRPLYN 401

BLAST of HG10021362 vs. NCBI nr
Match: XP_011657236.1 (glucan endo-1,3-beta-glucosidase 14 [Cucumis sativus] >KGN65539.1 hypothetical protein Csa_019797 [Cucumis sativus])

HSP 1 Score: 706.4 bits (1822), Expect = 1.4e-199
Identity = 367/412 (89.08%), Postives = 387/412 (93.93%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFHI LLFLLS SG FGGGLR  TSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD
Sbjct: 1   MFFHILLLFLLSLSG-FGGGLREVTSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TNP++LSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKP+VPAT+ITGIAVGNEV
Sbjct: 61  TNPEILSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPFVPATKITGIAVGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPAM+SIHTAL QLGLDT+IKISTPSSLAVL+ESYPPSAGSFKPEIT 
Sbjct: 121 FTDDDLTLMETLVPAMLSIHTALTQLGLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQ 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFLSTTKSPFWINAYPYFAYKD+P+S+PL+YVL NPNPGMIDPFT L YDNMLY
Sbjct: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDNPDSIPLQYVLLNPNPGMIDPFTNLRYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAKMGFGGIEVRISETGWPSKGD +ETGA +ENAA YNRNLLRRQM NEGT
Sbjct: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDSDETGACLENAAEYNRNLLRRQMRNEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKG-------SS 360
           PLRPNLRLEIYLFALFNE++KPGPTSERNYGL+QPDGTM YNVGL SSFKG       S 
Sbjct: 301 PLRPNLRLEIYLFALFNEDMKPGPTSERNYGLFQPDGTMVYNVGL-SSFKGFSSSSSSSP 360

Query: 361 SSSTSFSPSSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRRPLY 406
           SSS+SFSPSSISL+SSA TNSKGATMGYYQSLVYWMFVYLLTYQ+FMRRPLY
Sbjct: 361 SSSSSFSPSSISLTSSASTNSKGATMGYYQSLVYWMFVYLLTYQLFMRRPLY 410

BLAST of HG10021362 vs. NCBI nr
Match: XP_008444326.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 14-like [Cucumis melo])

HSP 1 Score: 704.5 bits (1817), Expect = 5.2e-199
Identity = 365/417 (87.53%), Postives = 388/417 (93.05%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFHI LLFLLS SG FGGGLRG TSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD
Sbjct: 1   MFFHILLLFLLSLSG-FGGGLRGVTSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TNP++LSAFANSKVEIIVTVENEMLAQLMDPQQALQWVT+RIKPYVPAT+ITGIAVGNEV
Sbjct: 61  TNPEILSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTSRIKPYVPATKITGIAVGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPA++SIHTAL QLGLDT+IKISTPSSLAVL+ESYPPSAGSFKPEIT 
Sbjct: 121 FTDDDLTLMETLVPAILSIHTALTQLGLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQ 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFLSTTKSPFWINAYPYFAYKD+P+S+PL+YVL NPNPGM+DPFT L YDNMLY
Sbjct: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDNPDSIPLQYVLLNPNPGMMDPFTNLRYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAKMGFGGIEVRISETGWPSKGDP+ETGA VENAA YNRNLLRRQM NEGT
Sbjct: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPDETGACVENAAEYNRNLLRRQMRNEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKG--------- 360
           PLRPNLRLEIYLFALFNE++KPGPTSERNYGL+QPDGTM YNVGL SSF G         
Sbjct: 301 PLRPNLRLEIYLFALFNEDMKPGPTSERNYGLFQPDGTMVYNVGL-SSFNGFSSSSSSSS 360

Query: 361 ---SSSSSTSFSPSSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRRPLY 406
              SS SS+SFSPSSISL+SSA TNSKGA+MGYYQSLVYWMFVYLLTYQ+F+RRPLY
Sbjct: 361 SSPSSPSSSSFSPSSISLTSSASTNSKGASMGYYQSLVYWMFVYLLTYQLFIRRPLY 415

BLAST of HG10021362 vs. NCBI nr
Match: KAG7012529.1 (Glucan endo-1,3-beta-glucosidase 11 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 656.0 bits (1691), Expect = 2.1e-184
Identity = 339/404 (83.91%), Postives = 371/404 (91.83%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFH+ LLFLL     FGG LRGA+SLGINYGQIG++LPSPDKVLDML AL+ITKVRIYD
Sbjct: 1   MFFHVLLLFLLH---GFGGELRGASSLGINYGQIGDDLPSPDKVLDMLMALKITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TN Q+LSAFANS VEIIVTVENEML +LMDPQQALQWV  RIKPYVPATR+TGIA+GNEV
Sbjct: 61  TNQQILSAFANSNVEIIVTVENEMLGELMDPQQALQWVATRIKPYVPATRVTGIAIGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPAM+SIHTAL+QLGLDT IK+STPSSLAVL+ESYPPSAGSFKPEITG
Sbjct: 121 FTDDDLTLMETLVPAMLSIHTALSQLGLDT-IKVSTPSSLAVLQESYPPSAGSFKPEITG 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFL+TTK+PFWINAYPYFAYKD+PN++PLEYV+FNPNPGMIDP+T LHYDNMLY
Sbjct: 181 IMSQFLQFLNTTKAPFWINAYPYFAYKDNPNNIPLEYVIFNPNPGMIDPYTNLHYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAK+GFGGIEVR+SETGWPSKGDPNE GAS+ENAANYNRNLLRRQMANEGT
Sbjct: 241 AQADAVLFAMAKLGFGGIEVRVSETGWPSKGDPNEVGASLENAANYNRNLLRRQMANEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFS 360
           PLRPNLRLE+YLFALFNEN+KPGPTSERNYGLYQPDGTMAYNVGL SSFKG     TSFS
Sbjct: 301 PLRPNLRLEVYLFALFNENMKPGPTSERNYGLYQPDGTMAYNVGL-SSFKG-----TSFS 360

Query: 361 P--SSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
           P  SSISL+S A    KG+T+GY +SLVY MFVYL+T +VF RR
Sbjct: 361 PSSSSISLTSMATREMKGSTIGYCRSLVYSMFVYLITSRVFTRR 394

BLAST of HG10021362 vs. NCBI nr
Match: KAG6573365.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 654.4 bits (1687), Expect = 6.2e-184
Identity = 338/404 (83.66%), Postives = 370/404 (91.58%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFH+ LLFLL     FGG LRGA+SLGINYGQIG++LPSPDKVLDML  L+ITKVRIYD
Sbjct: 1   MFFHVLLLFLLH---GFGGELRGASSLGINYGQIGDDLPSPDKVLDMLMGLKITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TN Q+LSAFANS VEIIVTVENEML +LMDPQQALQWV  RIKPYVPATR+TGIA+GNEV
Sbjct: 61  TNQQILSAFANSNVEIIVTVENEMLGELMDPQQALQWVATRIKPYVPATRVTGIAIGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPAM+SIHTAL+QLGLDT IK+STPSSLAVL+ESYPPSAGSFKPEITG
Sbjct: 121 FTDDDLTLMETLVPAMLSIHTALSQLGLDT-IKVSTPSSLAVLQESYPPSAGSFKPEITG 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFL+TTK+PFWINAYPYFAYKD+PN++PLEYV+FNPNPGMIDP+T LHYDNMLY
Sbjct: 181 IMSQFLQFLNTTKAPFWINAYPYFAYKDNPNNIPLEYVIFNPNPGMIDPYTNLHYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAK+GFGGIEVR+SETGWPSKGDPNE GAS+ENAANYNRNLLRRQMANEGT
Sbjct: 241 AQADAVLFAMAKLGFGGIEVRVSETGWPSKGDPNEVGASLENAANYNRNLLRRQMANEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFS 360
           PLRPNLRLE+YLFALFNEN+KPGPTSERNYGLYQPDGTMAYNVGL SSFKG     TSFS
Sbjct: 301 PLRPNLRLEVYLFALFNENMKPGPTSERNYGLYQPDGTMAYNVGL-SSFKG-----TSFS 360

Query: 361 P--SSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
           P  SSISL+S A    KG+T+GY +SLVY MFVYL+T +VF RR
Sbjct: 361 PSSSSISLTSMATREMKGSTIGYCRSLVYSMFVYLITSRVFTRR 394

BLAST of HG10021362 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 364.0 bits (933), Expect = 2.2e-99
Identity = 183/345 (53.04%), Postives = 241/345 (69.86%), Query Frame = 0

Query: 2   FFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDT 61
           FF + LL  L+ S    G       +GINYGQI NNLPSP +V  +L +L IT+V++YD 
Sbjct: 9   FFRVLLLLFLTLSERIKG-----QGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDA 68

Query: 62  NPQVLSAFANSKVEIIVTVENEMLAQL-MDPQQALQWVTARIKPYVPATRITGIAVGNEV 121
           +P VL +F+NS+V+ ++ + NE L  +  DP +A  W+  R++P++  TRIT I VGNE+
Sbjct: 69  DPNVLFSFSNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEI 128

Query: 122 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 181
           F  +D  L+++L+PAM S++ AL  LGL+  + +++  SL +L  SYPPS+GSFK E   
Sbjct: 129 FKTNDHVLIQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQ 188

Query: 182 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 241
            +   L F S  +SPF INAYP+FAYKD P  VPLEYVLF PN GM+DP T LHYDNML+
Sbjct: 189 YLQPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLF 248

Query: 242 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 301
           AQ DA+  A+  +G   IEVRISETGWPSKGD NE GAS ENAA YN NLL+     +GT
Sbjct: 249 AQVDALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGT 308

Query: 302 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGL 346
           P + ++ +++Y+FALFNENLKPGP SERNYGL+ PDG   YNVG+
Sbjct: 309 PAKQSVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGM 348

BLAST of HG10021362 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.1e-98
Identity = 184/366 (50.27%), Postives = 252/366 (68.85%), Query Frame = 0

Query: 2   FFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDT 61
           F    LLFL+S +          TS+G+NYGQIG+NLPSP  V+ ++ ++  TKV++YD 
Sbjct: 6   FHRSSLLFLISLTLIILP--TTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDA 65

Query: 62  NPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEVF 121
           NPQ+L AF+N+ +E I+ + NE L+++ DP +AL W+   + P++PAT IT I +GNE+ 
Sbjct: 66  NPQILKAFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEIL 125

Query: 122 TDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITGI 181
             +D +L   L+PAM  +H+AL   GL   I ++T  SL++L+ S+PPSAG F+P++   
Sbjct: 126 ALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDS 185

Query: 182 MSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYA 241
           ++  L+F   T SPF INAYP+FAYK +P  VPL++VLF PN G++DP T  HYDNML+A
Sbjct: 186 LTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFA 245

Query: 242 QADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANE--G 301
           Q DAV  A+A  GF  + V ISETGWPSKGD +E GA+ ENA  YN NL++  M+ +   
Sbjct: 246 QIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTK 305

Query: 302 TPLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVG--LISSFKGSSSSST 361
           TPL+PN  L IY+FALFNENLKPGPTSERNYGL++PDGT AY++G  L    +G+S   T
Sbjct: 306 TPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRGASGGGT 365

Query: 362 SFSPSS 364
               SS
Sbjct: 366 GGGNSS 369

BLAST of HG10021362 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 8.4e-91
Identity = 175/362 (48.34%), Postives = 235/362 (64.92%), Query Frame = 0

Query: 25  TSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPQVLSAFANSKVEIIVTVENEM 84
           +S+GINYGQ+ NNLP P  V+ +L ++  TKV++YD +PQ L AFA S  E+ V + NE 
Sbjct: 25  SSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRAFAGSGFELTVALGNEY 84

Query: 85  LAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEVFTDDDMTLMETLVPAMVSIHTALA 144
           LAQ+ DP +A  WV   ++ Y+P T+I  I VGNEV T +   L   L PAM SIH AL 
Sbjct: 85  LAQMSDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSALTAALFPAMQSIHGALV 144

Query: 145 QLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITGIMSQFLQFLSTTKSPFWINAYPYF 204
             GL+  I ++T  SLA+L+ SYPPSA SF+ ++ G ++  L F   T SP  INAYP+F
Sbjct: 145 DCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILDFHVKTGSPILINAYPFF 204

Query: 205 AYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYAQADAVLFAMAKMG--FGGIEVRI 264
           AY+++P  V L++VLF PN G  DP +  HYDNML+AQ DAV  A+  +G  +  + + +
Sbjct: 205 AYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVYHALDAVGISYKKVPIVV 264

Query: 265 SETGWPSKGDPNETGASVENAANYNRNLLRRQMANE-GTPLRPNLRLEIYLFALFNENLK 324
           SETGWPS GDP E GA+ +NA  YN NL++  M+ +  TP+RP   L I++FALFNEN+K
Sbjct: 265 SETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRPECDLTIFVFALFNENMK 324

Query: 325 PGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFSPSSISLSSSAPTNSKGATMGY 384
           PGPTSERNYGL+ PDGT  Y++G+ +S   SS S +S S    S      T    +  G 
Sbjct: 325 PGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGSGSSNSTGGSSSGGGGNTGGSSSGGGI 384

BLAST of HG10021362 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 286.2 bits (731), Expect = 5.8e-76
Identity = 165/389 (42.42%), Postives = 230/389 (59.13%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           M   I + FLL F   F         +G+NYGQ+ +NLP P + + +L +  I KVR+Y 
Sbjct: 1   MALSISIYFLLIFLSHFPSS-HAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYG 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQL-MDPQQALQWVTARIKPYVPATRITGIAVGNE 120
            +P ++ A A + V I++   N  +  L  DP  A QW+ + + P+ PA++I  I VGNE
Sbjct: 61  ADPAIIKALAGTGVGIVIGAANGDVPSLASDPNAATQWINSNVLPFYPASKIMLITVGNE 120

Query: 121 VFTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEIT 180
           +   +D  L+  L+PAM ++  AL  + L   IK+ST +S+ VL  S PPS+GSF     
Sbjct: 121 ILMSNDPNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQ 180

Query: 181 GIMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNML 240
             +   LQFLS T SPF IN YP+FAY+ DP    L + LF PN G +D  T + Y NM 
Sbjct: 181 TGLKGILQFLSDTGSPFAINPYPFFAYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMF 240

Query: 241 YAQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEG 300
            AQ DAV  A+  MGF  +E+ ++ETGW S+GD NE GASV+NA  YN NL+    +  G
Sbjct: 241 DAQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVG 300

Query: 301 TPLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSF 360
           TPL P   ++ Y+FAL++ENLKPGP+SER +GL++ D +M Y+VGL      + SSS+S 
Sbjct: 301 TPLMPGKPVDTYIFALYDENLKPGPSSERAFGLFKTDLSMVYDVGL------AKSSSSSQ 360

Query: 361 SPSSISLSSSAPTNSKGATMGYYQSLVYW 389
           +PS    SS      KGAT    Q+ + W
Sbjct: 361 TPSGKVTSSGWCVPKKGATNEELQASLDW 382

BLAST of HG10021362 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 5.5e-74
Identity = 158/364 (43.41%), Postives = 225/364 (61.81%), Query Frame = 0

Query: 11  LSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPQVLSAFA 70
           +S   F   G+  A+ +GI YG+  +NLPSP++V +++  L I  VRIYD N  VL AFA
Sbjct: 13  VSILAFLNFGM--ASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFA 72

Query: 71  NSKVEIIVTVEN-EMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEVFTDDDMTLM 130
           N+ +E+++ V N ++LA          W++  I PY P+T+IT I+VG EV T+      
Sbjct: 73  NTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEV-TEAPDNAT 132

Query: 131 ETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITGIMSQFLQFL 190
             ++PAM +IHTAL + GLD  IKIS+  SLA+L  S+PPS+ SF  + +  +   L+FL
Sbjct: 133 GLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFL 192

Query: 191 STTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYAQADAVLFA 250
              +SPF I+ YPY+AY+D    VPLEY LF  +  ++DP T L Y NM  AQ DA+ FA
Sbjct: 193 VENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFA 252

Query: 251 MAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGTPLRPNLRLE 310
           +  M F  ++V ++E+GWPSKG P ET A+ ENA  YN NL+R  + + GTP +P   ++
Sbjct: 253 LTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEID 312

Query: 311 IYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFSPSSISLSSS 370
           +YLF+LFNEN KPG  SERN+G++  +GT  Y +          S + S + +S S SSS
Sbjct: 313 VYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSS 372

Query: 371 APTN 374
              N
Sbjct: 373 PIIN 373

BLAST of HG10021362 vs. ExPASy TrEMBL
Match: A0A0A0LX16 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G436330 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.7e-200
Identity = 367/412 (89.08%), Postives = 387/412 (93.93%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFHI LLFLLS SG FGGGLR  TSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD
Sbjct: 1   MFFHILLLFLLSLSG-FGGGLREVTSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TNP++LSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKP+VPAT+ITGIAVGNEV
Sbjct: 61  TNPEILSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPFVPATKITGIAVGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPAM+SIHTAL QLGLDT+IKISTPSSLAVL+ESYPPSAGSFKPEIT 
Sbjct: 121 FTDDDLTLMETLVPAMLSIHTALTQLGLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQ 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFLSTTKSPFWINAYPYFAYKD+P+S+PL+YVL NPNPGMIDPFT L YDNMLY
Sbjct: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDNPDSIPLQYVLLNPNPGMIDPFTNLRYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAKMGFGGIEVRISETGWPSKGD +ETGA +ENAA YNRNLLRRQM NEGT
Sbjct: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDSDETGACLENAAEYNRNLLRRQMRNEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKG-------SS 360
           PLRPNLRLEIYLFALFNE++KPGPTSERNYGL+QPDGTM YNVGL SSFKG       S 
Sbjct: 301 PLRPNLRLEIYLFALFNEDMKPGPTSERNYGLFQPDGTMVYNVGL-SSFKGFSSSSSSSP 360

Query: 361 SSSTSFSPSSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRRPLY 406
           SSS+SFSPSSISL+SSA TNSKGATMGYYQSLVYWMFVYLLTYQ+FMRRPLY
Sbjct: 361 SSSSSFSPSSISLTSSASTNSKGATMGYYQSLVYWMFVYLLTYQLFMRRPLY 410

BLAST of HG10021362 vs. ExPASy TrEMBL
Match: A0A1S3BA63 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103487689 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.5e-199
Identity = 365/417 (87.53%), Postives = 388/417 (93.05%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFHI LLFLLS SG FGGGLRG TSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD
Sbjct: 1   MFFHILLLFLLSLSG-FGGGLRGVTSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TNP++LSAFANSKVEIIVTVENEMLAQLMDPQQALQWVT+RIKPYVPAT+ITGIAVGNEV
Sbjct: 61  TNPEILSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTSRIKPYVPATKITGIAVGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPA++SIHTAL QLGLDT+IKISTPSSLAVL+ESYPPSAGSFKPEIT 
Sbjct: 121 FTDDDLTLMETLVPAILSIHTALTQLGLDTTIKISTPSSLAVLQESYPPSAGSFKPEITQ 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFLSTTKSPFWINAYPYFAYKD+P+S+PL+YVL NPNPGM+DPFT L YDNMLY
Sbjct: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDNPDSIPLQYVLLNPNPGMMDPFTNLRYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAKMGFGGIEVRISETGWPSKGDP+ETGA VENAA YNRNLLRRQM NEGT
Sbjct: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPDETGACVENAAEYNRNLLRRQMRNEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKG--------- 360
           PLRPNLRLEIYLFALFNE++KPGPTSERNYGL+QPDGTM YNVGL SSF G         
Sbjct: 301 PLRPNLRLEIYLFALFNEDMKPGPTSERNYGLFQPDGTMVYNVGL-SSFNGFSSSSSSSS 360

Query: 361 ---SSSSSTSFSPSSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRRPLY 406
              SS SS+SFSPSSISL+SSA TNSKGA+MGYYQSLVYWMFVYLLTYQ+F+RRPLY
Sbjct: 361 SSPSSPSSSSFSPSSISLTSSASTNSKGASMGYYQSLVYWMFVYLLTYQLFIRRPLY 415

BLAST of HG10021362 vs. ExPASy TrEMBL
Match: A0A6J1KIL4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111495568 PE=3 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 6.7e-184
Identity = 338/402 (84.08%), Postives = 365/402 (90.80%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           M  H+ LLFLLS S        GATSLGINYGQIGNNLP PDKVL+MLTALRITKVRIYD
Sbjct: 1   MLLHLALLFLLSIS--------GATSLGINYGQIGNNLPPPDKVLNMLTALRITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TNPQVLSAFANSKV +IVT+EN+MLA+LM+PQQALQWVT RIKPYVPAT+IT IAVGNEV
Sbjct: 61  TNPQVLSAFANSKVNLIVTIENQMLAELMNPQQALQWVTERIKPYVPATQITEIAVGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           F+++DMTLMETL+PAM+SIHTAL QLGL +SIK+STPSSLAVLEESYPPSAGSFKPEITG
Sbjct: 121 FSENDMTLMETLIPAMLSIHTALTQLGL-SSIKLSTPSSLAVLEESYPPSAGSFKPEITG 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IM+QFL+FLS TK+PFWINAYPYFAYKDDPN +PLEYVLFNPNPGM+DPFTRLHYDNMLY
Sbjct: 181 IMTQFLEFLSRTKAPFWINAYPYFAYKDDPNGIPLEYVLFNPNPGMVDPFTRLHYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAV FAMAKMGF GIEVRISETGWPSKGDPNE GA+++NAANYNRNLLRRQMANEGT
Sbjct: 241 AQADAVYFAMAKMGFEGIEVRISETGWPSKGDPNENGATLQNAANYNRNLLRRQMANEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFS 360
           P+RPN+RLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGL SSFKG+        
Sbjct: 301 PMRPNIRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGL-SSFKGT-------- 360

Query: 361 PSSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
            SSISL+SSAPT  KG  MGYYQSLVYWMFVYLLTYQVFMRR
Sbjct: 361 -SSISLTSSAPT--KGGIMGYYQSLVYWMFVYLLTYQVFMRR 381

BLAST of HG10021362 vs. ExPASy TrEMBL
Match: A0A6J1K666 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111490433 PE=3 SV=1)

HSP 1 Score: 652.9 bits (1683), Expect = 8.8e-184
Identity = 337/404 (83.42%), Postives = 370/404 (91.58%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFH+ LLFLL     FGG LRGA+SLGINYGQIG++LPSPDKVLDML AL+ITKVRIYD
Sbjct: 1   MFFHVLLLFLLH---GFGGELRGASSLGINYGQIGDDLPSPDKVLDMLMALKITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TN Q+LSAFANS VEIIVTVENEML +LMDPQQALQWV  +IKPYVPATR+TGIA+GNEV
Sbjct: 61  TNQQILSAFANSDVEIIVTVENEMLGELMDPQQALQWVATQIKPYVPATRVTGIAIGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPAM+SIHTAL+QLGLDT IK+STPSSLAVL+ESYPPSAGSFKPEITG
Sbjct: 121 FTDDDLTLMETLVPAMLSIHTALSQLGLDT-IKVSTPSSLAVLQESYPPSAGSFKPEITG 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFL+TT +PFWINAYPYFAYKD+PN++PLEYV+FNPNPGMIDP+T LHYDNMLY
Sbjct: 181 IMSQFLQFLNTTNAPFWINAYPYFAYKDNPNNIPLEYVIFNPNPGMIDPYTNLHYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAK+GFGGIEVR+SETGWPSKGDPNE GAS+ENAANYNRNLLRRQMANEGT
Sbjct: 241 AQADAVLFAMAKLGFGGIEVRVSETGWPSKGDPNEVGASLENAANYNRNLLRRQMANEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFS 360
           PLRPNLRLE+YLFALFNEN+KPGPTSERNYGLYQPDGTMAYNVGL SSFKG     TSFS
Sbjct: 301 PLRPNLRLEVYLFALFNENMKPGPTSERNYGLYQPDGTMAYNVGL-SSFKG-----TSFS 360

Query: 361 P--SSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
           P  SSISL+S A    KG+T+GY +SLVY MFVYL+T +VF RR
Sbjct: 361 PSSSSISLTSKATREMKGSTIGYCRSLVYLMFVYLITSRVFTRR 394

BLAST of HG10021362 vs. ExPASy TrEMBL
Match: A0A6J1GSZ9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111457256 PE=3 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 8.2e-182
Identity = 335/404 (82.92%), Postives = 368/404 (91.09%), Query Frame = 0

Query: 1   MFFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYD 60
           MFFH+ LLFLL     FGG LRGA+SLGINYGQIG++LPSPDKVLDML AL+ITKVRIYD
Sbjct: 1   MFFHVLLLFLLH---GFGGELRGASSLGINYGQIGDDLPSPDKVLDMLMALKITKVRIYD 60

Query: 61  TNPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEV 120
           TN Q+LSAFANS VEIIVTVENEML +L +PQQALQWV  RIKPYVPATR+TGIA+GNEV
Sbjct: 61  TNQQILSAFANSNVEIIVTVENEMLGELTNPQQALQWVATRIKPYVPATRVTGIAIGNEV 120

Query: 121 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 180
           FTDDD+TLMETLVPAM+SIH AL+QLGLDT IK+STPSSLAVL+ESYPPSAGSFKPEITG
Sbjct: 121 FTDDDLTLMETLVPAMLSIHIALSQLGLDT-IKVSTPSSLAVLQESYPPSAGSFKPEITG 180

Query: 181 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 240
           IMSQFLQFL+TTK+PFWINAYPYFAYKD+ N++PLEYV+FNPNPGMIDP+T LHYDNMLY
Sbjct: 181 IMSQFLQFLNTTKAPFWINAYPYFAYKDNSNNIPLEYVIFNPNPGMIDPYTNLHYDNMLY 240

Query: 241 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 300
           AQADAVLFAMAK+GFGGIEVR+SETGWPSKGDPNE GAS+ENAANYNRNLLRRQMANEGT
Sbjct: 241 AQADAVLFAMAKLGFGGIEVRVSETGWPSKGDPNEVGASLENAANYNRNLLRRQMANEGT 300

Query: 301 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFS 360
           PLRPNLRLE+YLFALFNEN+KPGPTSERNYGLYQPDGTMAYNVGL SSFKG     TSFS
Sbjct: 301 PLRPNLRLEVYLFALFNENMKPGPTSERNYGLYQPDGTMAYNVGL-SSFKG-----TSFS 360

Query: 361 P--SSISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
           P  SSISL+S A    KG+T+GY +SLVY MFVYL+T +VF RR
Sbjct: 361 PSSSSISLTSMATREMKGSTIGYCRSLVYSMFVYLITSRVFTRR 394

BLAST of HG10021362 vs. TAIR 10
Match: AT1G30080.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 512.3 bits (1318), Expect = 3.6e-145
Identity = 263/396 (66.41%), Postives = 318/396 (80.30%), Query Frame = 0

Query: 7   LLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNPQVL 66
           LLF L+F    G   +  +SLGINYGQ+G+NLP PDKVL +L++L I K RIYDTNP+VL
Sbjct: 17  LLFSLTFLE-HGLLFQRVSSLGINYGQVGDNLPPPDKVLQLLSSLHINKTRIYDTNPRVL 76

Query: 67  SAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEVFTDDDM 126
           ++FANS +E+ VTVENEML  L+DPQQALQWVT RIKPY PAT+I GIAVGNE++TDDD 
Sbjct: 77  TSFANSNIELFVTVENEMLPSLVDPQQALQWVTTRIKPYFPATKIGGIAVGNELYTDDDS 136

Query: 127 TLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITGIMSQFL 186
           +L+  L+PAM+SIH AL Q GLD  I++STP+SL+VL+ESYPPSAG F+PE+ G+M+Q L
Sbjct: 137 SLIGYLMPAMMSIHGALVQTGLDKYIQVSTPNSLSVLQESYPPSAGCFRPEVAGVMTQLL 196

Query: 187 QFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYAQADAV 246
            FL  T SPFWINAYPYFAYKD P  +PL+YVLFNPNPGM+DP+T+  YDNMLYAQ DAV
Sbjct: 197 GFLRNTNSPFWINAYPYFAYKDSPTKIPLDYVLFNPNPGMVDPYTKYRYDNMLYAQVDAV 256

Query: 247 LFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGTPLRPNL 306
           +FAMA++GF  IEV +SETGWPSKGD +E GA+V NAA YN+N+LRRQ+ NEGTPLRPNL
Sbjct: 257 IFAMARLGFKDIEVGVSETGWPSKGDGDEVGATVANAAVYNKNILRRQLQNEGTPLRPNL 316

Query: 307 RLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFSPSSISL 366
             ++YLFALFNE+LKPGPTSERNYGLYQPD TM YNVGL+SS   +S+S+TS S S ISL
Sbjct: 317 SFDVYLFALFNEDLKPGPTSERNYGLYQPDETMTYNVGLLSSSSLTSTSTTS-STSIISL 376

Query: 367 SSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
           +SSA T  K       Q L+YW  VYLL   + +RR
Sbjct: 377 TSSASTALKKGK----QRLMYWTCVYLLAIHMLIRR 406

BLAST of HG10021362 vs. TAIR 10
Match: AT4G18340.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 493.8 bits (1270), Expect = 1.3e-139
Identity = 254/399 (63.66%), Postives = 311/399 (77.94%), Query Frame = 0

Query: 4   HIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDTNP 63
           H+ LL  L FSG     L+  TSLGINYGQ+GNNLPSPDKV+++L +LRITK RIYDTNP
Sbjct: 14  HVFLLLSLVFSGNI---LQTVTSLGINYGQVGNNLPSPDKVINLLRSLRITKTRIYDTNP 73

Query: 64  QVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEVFTD 123
           Q+LSAFANS +EIIVT+EN++L  L DPQQA QWV + IKPYVPATRITGI VGNE+FTD
Sbjct: 74  QILSAFANSNIEIIVTIENQVLPLLQDPQQATQWVDSHIKPYVPATRITGIMVGNELFTD 133

Query: 124 DDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITGIMS 183
           DD +L+  ++PA+++IH AL QLGLD  I++S+PSSLAVL ESYPPSAGSFKPE++ +M 
Sbjct: 134 DDSSLIGYMMPAIINIHKALVQLGLDRYIQVSSPSSLAVLGESYPPSAGSFKPEVSSVMQ 193

Query: 184 QFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYAQA 243
           Q L FL  TKSPFWINAYPYFAYKD+P  +P++YVLFN N GM DP TRLHYDNM+YAQ 
Sbjct: 194 QLLDFLEATKSPFWINAYPYFAYKDNPQEIPVDYVLFNRNIGMTDPNTRLHYDNMMYAQV 253

Query: 244 DAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGTPLR 303
           DAV FA AK+G+  IEVR++ETGWPSKGD  E GAS  NAA YNRNL+ RQ A EGTP R
Sbjct: 254 DAVAFAAAKLGYRNIEVRVAETGWPSKGDVGEIGASPVNAATYNRNLMMRQFAGEGTPAR 313

Query: 304 PNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGLISSFKGSSSSSTSFSPSS 363
            N RL++Y+FALFNE++KPGPTSE+NYG++QPDG++AYN+G       S+ S+T+ +  S
Sbjct: 314 RNARLDVYIFALFNEDMKPGPTSEKNYGIFQPDGSLAYNLGF------STMSTTTANSES 373

Query: 364 ISLSSSAPTNSKGATMGYYQSLVYWMFVYLLTYQVFMRR 403
           ++ SSSA T +K       +SL YW  + L   QV M R
Sbjct: 374 VTYSSSA-TKAK-------RSLEYWTILILAMVQVVMLR 395

BLAST of HG10021362 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-100
Identity = 183/345 (53.04%), Postives = 241/345 (69.86%), Query Frame = 0

Query: 2   FFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDT 61
           FF + LL  L+ S    G       +GINYGQI NNLPSP +V  +L +L IT+V++YD 
Sbjct: 9   FFRVLLLLFLTLSERIKG-----QGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDA 68

Query: 62  NPQVLSAFANSKVEIIVTVENEMLAQL-MDPQQALQWVTARIKPYVPATRITGIAVGNEV 121
           +P VL +F+NS+V+ ++ + NE L  +  DP +A  W+  R++P++  TRIT I VGNE+
Sbjct: 69  DPNVLFSFSNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEI 128

Query: 122 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 181
           F  +D  L+++L+PAM S++ AL  LGL+  + +++  SL +L  SYPPS+GSFK E   
Sbjct: 129 FKTNDHVLIQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQ 188

Query: 182 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 241
            +   L F S  +SPF INAYP+FAYKD P  VPLEYVLF PN GM+DP T LHYDNML+
Sbjct: 189 YLQPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLF 248

Query: 242 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 301
           AQ DA+  A+  +G   IEVRISETGWPSKGD NE GAS ENAA YN NLL+     +GT
Sbjct: 249 AQVDALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGT 308

Query: 302 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGL 346
           P + ++ +++Y+FALFNENLKPGP SERNYGL+ PDG   YNVG+
Sbjct: 309 PAKQSVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGM 348

BLAST of HG10021362 vs. TAIR 10
Match: AT2G27500.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.6e-100
Identity = 183/345 (53.04%), Postives = 241/345 (69.86%), Query Frame = 0

Query: 2   FFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDT 61
           FF + LL  L+ S    G       +GINYGQI NNLPSP +V  +L +L IT+V++YD 
Sbjct: 9   FFRVLLLLFLTLSERIKG-----QGVGINYGQIANNLPSPARVAVLLRSLNITRVKLYDA 68

Query: 62  NPQVLSAFANSKVEIIVTVENEMLAQL-MDPQQALQWVTARIKPYVPATRITGIAVGNEV 121
           +P VL +F+NS+V+ ++ + NE L  +  DP +A  W+  R++P++  TRIT I VGNE+
Sbjct: 69  DPNVLFSFSNSQVDFMIGLGNEYLQNMSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEI 128

Query: 122 FTDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITG 181
           F  +D  L+++L+PAM S++ AL  LGL+  + +++  SL +L  SYPPS+GSFK E   
Sbjct: 129 FKTNDHVLIQSLLPAMKSVYAALTNLGLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQ 188

Query: 182 IMSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLY 241
            +   L F S  +SPF INAYP+FAYKD P  VPLEYVLF PN GM+DP T LHYDNML+
Sbjct: 189 YLQPLLDFHSQIESPFLINAYPFFAYKDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLF 248

Query: 242 AQADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANEGT 301
           AQ DA+  A+  +G   IEVRISETGWPSKGD NE GAS ENAA YN NLL+     +GT
Sbjct: 249 AQVDALYSAIKTLGHTDIEVRISETGWPSKGDENEIGASPENAALYNGNLLKLIQQRKGT 308

Query: 302 PLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVGL 346
           P + ++ +++Y+FALFNENLKPGP SERNYGL+ PDG   YNVG+
Sbjct: 309 PAKQSVPIDVYVFALFNENLKPGPVSERNYGLFYPDGKPVYNVGM 348

BLAST of HG10021362 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 361.7 bits (927), Expect = 7.8e-100
Identity = 184/366 (50.27%), Postives = 252/366 (68.85%), Query Frame = 0

Query: 2   FFHIPLLFLLSFSGFFGGGLRGATSLGINYGQIGNNLPSPDKVLDMLTALRITKVRIYDT 61
           F    LLFL+S +          TS+G+NYGQIG+NLPSP  V+ ++ ++  TKV++YD 
Sbjct: 6   FHRSSLLFLISLTLIILP--TTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDA 65

Query: 62  NPQVLSAFANSKVEIIVTVENEMLAQLMDPQQALQWVTARIKPYVPATRITGIAVGNEVF 121
           NPQ+L AF+N+ +E I+ + NE L+++ DP +AL W+   + P++PAT IT I +GNE+ 
Sbjct: 66  NPQILKAFSNTGIEFIIGLGNEYLSKMKDPSKALTWIKQNVTPFLPATNITCITIGNEIL 125

Query: 122 TDDDMTLMETLVPAMVSIHTALAQLGLDTSIKISTPSSLAVLEESYPPSAGSFKPEITGI 181
             +D +L   L+PAM  +H+AL   GL   I ++T  SL++L+ S+PPSAG F+P++   
Sbjct: 126 ALNDSSLTTNLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDS 185

Query: 182 MSQFLQFLSTTKSPFWINAYPYFAYKDDPNSVPLEYVLFNPNPGMIDPFTRLHYDNMLYA 241
           ++  L+F   T SPF INAYP+FAYK +P  VPL++VLF PN G++DP T  HYDNML+A
Sbjct: 186 LTPILEFHRKTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFA 245

Query: 242 QADAVLFAMAKMGFGGIEVRISETGWPSKGDPNETGASVENAANYNRNLLRRQMANE--G 301
           Q DAV  A+A  GF  + V ISETGWPSKGD +E GA+ ENA  YN NL++  M+ +   
Sbjct: 246 QIDAVYSALAAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTK 305

Query: 302 TPLRPNLRLEIYLFALFNENLKPGPTSERNYGLYQPDGTMAYNVG--LISSFKGSSSSST 361
           TPL+PN  L IY+FALFNENLKPGPTSERNYGL++PDGT AY++G  L    +G+S   T
Sbjct: 306 TPLKPNNDLSIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGFALNDVVRGASGGGT 365

Query: 362 SFSPSS 364
               SS
Sbjct: 366 GGGNSS 369