HG10021127 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021127 vs. NCBI nr
Match: XP_016898901.1 (PREDICTED: (3S,6E)-nerolidol synthase 1-like [Cucumis melo])

HSP 1 Score: 457.6 bits (1176), Expect = 8.4e-125
Identity = 249/422 (59.00%), Postives = 275/422 (65.17%), Query Frame = 0

Query: 1   MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
           M+K GELNK+ IED+EGLTSVYEAWQLCLPGE+KL+KIG+ SGHIL+T+IGNL++ MAK+
Sbjct: 179 MDKNGELNKKGIEDIEGLTSVYEAWQLCLPGEDKLDKIGEFSGHILRTQIGNLENGMAKH 238

Query: 61  VGDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQL 120
           VGD LTNPFHK L KL++KNYF +    Q TNKWIFAFQ LAKLDFNRVQ LY+ E+SQL
Sbjct: 239 VGDTLTNPFHKSLAKLYIKNYFGY----QATNKWIFAFQNLAKLDFNRVQKLYRHEISQL 298

Query: 121 FL---------------------------------------------------------- 180
            L                                                          
Sbjct: 299 LLWWKKAGLCEELKFSRDQPLKWYICSVVCLADPKFSEERVELTKSISFIYLLDDIYDLY 358

Query: 181 ---------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK 240
                          WDL A+HSLPHPMK CYIKLYETT EL HKFSL HGWNPIHSLQK
Sbjct: 359 GSIEELRLFTQAIQRWDLEAVHSLPHPMKFCYIKLYETTKELGHKFSLKHGWNPIHSLQK 418

Query: 241 S------------------------------------------HVFFLLGQGINNKTVQL 300
           S                                          H+FFLLGQGINNKT+QL
Sbjct: 419 SVPFVTCEAFLVEAEWFASGHFPSAKEYLENGAVSSGVHVVLEHIFFLLGQGINNKTLQL 478

Query: 301 LNTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMI 307
           L+TNP  VSSTASILRLSDDLGSAKDENQEGYDGSYI+YY KEN EISVDSARQRVIHMI
Sbjct: 479 LDTNPATVSSTASILRLSDDLGSAKDENQEGYDGSYIDYYLKENPEISVDSARQRVIHMI 538

BLAST of HG10021127 vs. NCBI nr
Match: XP_038893851.1 (LOW QUALITY PROTEIN: (3S,6E)-nerolidol synthase 1-like [Benincasa hispida])

HSP 1 Score: 449.9 bits (1156), Expect = 1.8e-122
Identity = 252/420 (60.00%), Postives = 269/420 (64.05%), Query Frame = 0

Query: 1   MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
           M KEGELNK+V EDMEGLTS+YEA QLC PGEE LEKIGK SG ILKT++ N D+  AK+
Sbjct: 175 MNKEGELNKKVSEDMEGLTSLYEASQLCFPGEETLEKIGKFSGQILKTQMENPDNGTAKH 234

Query: 61  VGDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQL 120
           VGDAL NPFHK LG+L VKNYF F       NKWI  FQ+LAKLDFNRVQ LYQRE+SQL
Sbjct: 235 VGDALANPFHKSLGRLMVKNYFGFP---ATHNKWISVFQQLAKLDFNRVQKLYQREISQL 294

Query: 121 FL---------------------------------------------------------- 180
           FL                                                          
Sbjct: 295 FLWWKMTGLSEEIKFGRDQPLKWYVCSLAFLADPKFSEERVELSKSLSFIYLIDDIYDLY 354

Query: 181 ---------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK 240
                          W LAAIH LPHPMKIC+IKLYETTNELSH FSL HGWNPIHSLQK
Sbjct: 355 GSIEELRLFTKAIQRWYLAAIHGLPHPMKICFIKLYETTNELSHMFSLKHGWNPIHSLQK 414

Query: 241 S-----------------------------------------HVFFLLGQGINNKTVQLL 300
           S                                         H+FFLLGQGINNKTVQLL
Sbjct: 415 SWASLCEAFLVEAEWFASNHFPSAKEYLENGMVSSGVHVVLAHIFFLLGQGINNKTVQLL 474

Query: 301 NTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMIS 307
           +TNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYI+YY  EN EISVDSARQR+IHMIS
Sbjct: 475 DTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIKYYMNENPEISVDSARQRIIHMIS 534

BLAST of HG10021127 vs. NCBI nr
Match: XP_011648431.2 ((3S,6E)-nerolidol synthase 1 [Cucumis sativus] >KAE8652684.1 hypothetical protein Csa_013083 [Cucumis sativus])

HSP 1 Score: 439.9 bits (1130), Expect = 1.8e-119
Identity = 246/419 (58.71%), Postives = 268/419 (63.96%), Query Frame = 0

Query: 3   KEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYVG 62
           K GELNK+V ED+EGLTSVYEA QLCLPGEEKLE IG+  GHIL+T+I NL++ MAK+VG
Sbjct: 184 KNGELNKKVFEDIEGLTSVYEACQLCLPGEEKLEIIGEFCGHILRTQIRNLENGMAKHVG 243

Query: 63  DALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQLFL 122
           D LTNPFHK L KLF+K +F F    Q TNKWIFAFQKLAKLDFNR Q  YQ E+SQL L
Sbjct: 244 DTLTNPFHKSLAKLFIKTHFGF----QATNKWIFAFQKLAKLDFNRAQKSYQHEISQLIL 303

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 304 WWKKTGLSEELKFSRHQPLKWYICSLVCLGDPKFSEERVELTKSISFIYLLDDIYDLYGS 363

Query: 183 -------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS- 242
                        W+L AI+SLPHPMK CYIKLYETTNELSHKFSL HGWNPIH LQKS 
Sbjct: 364 IEELRLFTQAIQRWNLEAINSLPHPMKFCYIKLYETTNELSHKFSLKHGWNPIHCLQKSW 423

Query: 243 ----------------------------------------HVFFLLGQGINNKTVQLLNT 302
                                                   H FFLLGQGINNKT+QLL+T
Sbjct: 424 TSLCEAFLVEAEWFASGHFPSAKEYLENGEVSSGVHVVLEHTFFLLGQGINNKTLQLLDT 483

Query: 303 NPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISDA 307
           NP IVSSTASILRLSDDLGSAKDENQEGYDGSYI+YY KEN EISVDSA+QRV HMISDA
Sbjct: 484 NPAIVSSTASILRLSDDLGSAKDENQEGYDGSYIDYYMKENPEISVDSAQQRVNHMISDA 543

BLAST of HG10021127 vs. NCBI nr
Match: XP_022140145.1 ((3S,6E)-nerolidol synthase 1-like [Momordica charantia])

HSP 1 Score: 400.6 bits (1028), Expect = 1.2e-107
Identity = 229/422 (54.27%), Postives = 260/422 (61.61%), Query Frame = 0

Query: 1   MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
           M+KEG L KEV ED+EGLT++YEA QLCLP E+KLEKIG  SGH+LK  IGNLDD  A +
Sbjct: 175 MDKEGHLGKEVREDIEGLTALYEASQLCLPQEDKLEKIGNLSGHMLKISIGNLDDHWANH 234

Query: 61  V-GDALTNPFHKCLGKLFVKNYFRFQP-QLQPTNKWIFAFQKLAKLDFNRVQNLYQREMS 120
           V  + L NPFHK L    VKNYF F+   L+ TNKWI+ FQK+AKL+FNRVQ+++Q+E+S
Sbjct: 235 VISNTLANPFHKSLANFLVKNYFGFEDYHLRATNKWIYVFQKMAKLEFNRVQSIHQQEIS 294

Query: 121 QL-----------------------------FL--------------------------- 180
           QL                             FL                           
Sbjct: 295 QLSRWWGQTGLSKELEFARDQPLKWYICSLAFLTDPIFSEERVDLAKSLSFIYLVDDIYD 354

Query: 181 -----------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSL 240
                            WDLAAI +LPH MKICYIKLYETTNE+S+KF   HGWNPI+SL
Sbjct: 355 LYGSLEQLRLFTKAIQRWDLAAIDNLPHSMKICYIKLYETTNEISNKFFQKHGWNPINSL 414

Query: 241 QKS-----------------------------------------HVFFLLGQGINNKTVQ 300
           QKS                                         H+FFLLGQGIN +T  
Sbjct: 415 QKSWATLCDAFLVEAEWFASGNLPSAKEYLRNGALSCGVHVVLAHIFFLLGQGINKQTEL 474

Query: 301 LLNTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHM 307
           LL++NPGIVSSTA+ILRL DDLGSAKDENQEGYDGSYIE Y KEN EI +DS RQRV HM
Sbjct: 475 LLDSNPGIVSSTATILRLCDDLGSAKDENQEGYDGSYIECYMKENPEICLDSTRQRVKHM 534

BLAST of HG10021127 vs. NCBI nr
Match: XP_023520214.1 (uncharacterized protein LOC111783517 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 354.0 bits (907), Expect = 1.3e-93
Identity = 206/421 (48.93%), Postives = 246/421 (58.43%), Query Frame = 0

Query: 1    MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
            ++ EG L+ + IE++EGLTS+YEA QLC P +EKLEKIG  S  ILK    N DD++ K+
Sbjct: 762  IDHEGLLDTKFIENIEGLTSLYEASQLCFPEDEKLEKIGNFSARILKKLAWNRDDNLGKH 821

Query: 61   VGDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQL 120
            V  A+ NPFHK L K  V +YF      Q  NKWI+ FQ +AKLDFNR+QNL++ E+SQ 
Sbjct: 822  VRKAVANPFHKSLVKFVVMDYF----GSQSPNKWIYVFQPMAKLDFNRLQNLHRLELSQF 881

Query: 121  FL---------------------------------------------------------- 180
             +                                                          
Sbjct: 882  IMWWKKTGLCEELKFARDQPLKWYICSMAVLADPMFSEERVELTKSISFIYLIDDIYDVY 941

Query: 181  ---------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK 240
                           WDLAA+  LP+PMK C IKL+ETTNE+ HKF L HGWNPI+S+ K
Sbjct: 942  GSLEELRLFTKAIQRWDLAALDGLPNPMKFCIIKLHETTNEICHKFYLKHGWNPINSIHK 1001

Query: 241  S-----------------------------------------HVFFLLGQGINNKTVQLL 300
            S                                         H+FFLLGQG++N+ V LL
Sbjct: 1002 SWVSLCEAFLVEAEWFGSSHLPSAEEYLENGAVSSGVHVVLAHIFFLLGQGVSNEAV-LL 1061

Query: 301  NTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMIS 307
            ++NP IVSSTASILRLSDDLGSAKDENQEG+DGSYIE Y KEN EISVDS R+R+ HMIS
Sbjct: 1062 SSNPDIVSSTASILRLSDDLGSAKDENQEGHDGSYIECYMKENPEISVDSTRERISHMIS 1121

BLAST of HG10021127 vs. ExPASy Swiss-Prot
Match: P0CV96 ((3S,6E)-nerolidol synthase 1, chloroplastic OS=Fragaria vesca OX=57918 PE=1 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.4e-66
Identity = 152/419 (36.28%), Postives = 212/419 (50.60%), Query Frame = 0

Query: 2   EKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYV 61
           + +G   + + ED++GL S+YEA QL   GE+ L +  K SGH+LKT + +LD   A+ V
Sbjct: 165 DSKGTFKQVLGEDIKGLMSLYEASQLGTEGEDTLVEAEKFSGHLLKTSLSHLDRHRARIV 224

Query: 62  GDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQLF 121
           G+ L NP  K L     +N+F      Q TN W+   +++AK DFN V++++Q+E+ Q+ 
Sbjct: 225 GNTLRNPHRKSLASFMARNFF---VTSQATNSWLNLLKEVAKTDFNMVRSVHQKEIVQIS 284

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 285 KWWKELGLVKELKFARDQPLKWYTWSMAGLTDPKLSEERVELTKPISFVYLIDDIFDVYG 344

Query: 182 -------------LWDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS 241
                         W++ AI  LP  MKIC+  LY+ TNE S K    HGWNP+ SL+ S
Sbjct: 345 TLDDLILFTEAVNRWEITAIDHLPDYMKICFKALYDMTNEFSCKVYQKHGWNPLRSLKIS 404

Query: 242 -----------------------------------------HVFFLLGQGINNKTVQLLN 301
                                                    H+FFLLGQ I  K+V+LLN
Sbjct: 405 WASLCNAFLVEAKWFASGQLPKSEEYLKNGIVSSGVNVGLVHMFFLLGQNITRKSVELLN 464

Query: 302 TNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISD 307
             P ++SS+A+ILRL DDLGSAKDENQ+G DGSY+  Y +E+   S++ AR++ I+MISD
Sbjct: 465 ETPAMISSSAAILRLWDDLGSAKDENQDGNDGSYVRCYLEEHEGCSIEEAREKTINMISD 524

BLAST of HG10021127 vs. ExPASy Swiss-Prot
Match: P0CV94 ((3S,6E)-nerolidol synthase 1 OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.4e-66
Identity = 153/419 (36.52%), Postives = 210/419 (50.12%), Query Frame = 0

Query: 2   EKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYV 61
           + +G   + + ED++GL S+YEA QL   GE+ L +  K SGH+LKT + +LD    + V
Sbjct: 104 DSKGTFKQVLGEDIKGLMSLYEASQLGTEGEDILVEAEKFSGHLLKTSLSHLDHHRVRIV 163

Query: 62  GDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQLF 121
            + L NP HK L     +N+F      Q TN W+   +++AK DFN V++L+Q E+ Q+ 
Sbjct: 164 ANTLRNPHHKSLAPFMARNFF---VTSQATNSWLNLLKEVAKTDFNMVRSLHQNEIVQMS 223

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 224 KWWKELGLAKELKFARDQPLKWYIWSMACLTDPKLSEERVELTKPISFVYLIDDIFDVYG 283

Query: 182 -------------LWDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS 241
                         W++ AI  LP  MKIC+  LY+ TNE S K  L HGWNP+ SL+ S
Sbjct: 284 TLDDLILFTEAVNRWEITAIDHLPDYMKICFKALYDMTNEFSSKVYLKHGWNPLQSLKIS 343

Query: 242 -----------------------------------------HVFFLLGQGINNKTVQLLN 301
                                                    H+FFLLGQ I  K+V+LLN
Sbjct: 344 WASLCNAFLVEAKWFASGKLPKSEEYLKNGIVSSGVNVVLVHMFFLLGQNITRKSVELLN 403

Query: 302 TNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISD 307
             P I+SS+A+ILRL DDLGSAKDENQ+G DGSY+  Y +E+   S++ AR++ I+MISD
Sbjct: 404 ETPAIISSSAAILRLWDDLGSAKDENQDGNDGSYVRCYLEEHEGCSIEEAREKTINMISD 463

BLAST of HG10021127 vs. ExPASy Swiss-Prot
Match: P0CV95 ((3S,6E)-nerolidol synthase 2, chloroplastic/mitochondrial OS=Fragaria ananassa OX=3747 PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 4.1e-66
Identity = 152/419 (36.28%), Postives = 210/419 (50.12%), Query Frame = 0

Query: 2   EKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYV 61
           + +G   + + ED++GL S+YEA QL   GE+ L +  K SGH+LKT + +LD   A+ V
Sbjct: 163 DSKGTFKQVLGEDIKGLMSLYEASQLGTEGEDTLVEAEKFSGHLLKTSLSHLDHHHARIV 222

Query: 62  GDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQLF 121
           G+ L NP HK L     +N+F      Q TN W+   + +AK DFN V++L+Q E+ Q+ 
Sbjct: 223 GNTLRNPHHKSLASFMARNFF---VTTQATNSWLNLLKDVAKTDFNMVRSLHQNEIVQIS 282

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 283 KWWKELGLAKELKFARDQPQKWYIWSMACLTDPKLSEERVELTKPISFVYLIDDIFDVYG 342

Query: 182 -------------LWDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS 241
                         W++ AI  LP  MKIC+  LY+ TNE+S K    HGWNP+ SL+ S
Sbjct: 343 TLDDLILFTEAVNRWEITAIDHLPDYMKICFKALYDMTNEISCKVYQKHGWNPLQSLKIS 402

Query: 242 -----------------------------------------HVFFLLGQGINNKTVQLLN 301
                                                    H+FF+LGQ I  K+V+LLN
Sbjct: 403 WASLCNAFLVEAKWFASGQLPKSKEYLKNGIVSSGVNVVLVHMFFILGQNITTKSVELLN 462

Query: 302 TNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISD 307
             P ++SS+A+ILRL DDLGSAKDENQ+G DGSY+  Y +E+   S++ AR++ I+MISD
Sbjct: 463 ETPAMISSSAAILRLWDDLGSAKDENQDGNDGSYVRCYLEEHEGCSIEEAREKTINMISD 522

BLAST of HG10021127 vs. ExPASy Swiss-Prot
Match: B9RHX7 (Probable terpene synthase 4 OS=Ricinus communis OX=3988 GN=TPS4 PE=3 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 4.7e-62
Identity = 147/419 (35.08%), Postives = 210/419 (50.12%), Query Frame = 0

Query: 3   KEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYVG 62
           +EG+  +E+  D++GL  +YEA QL +  E  L++ G  S H L     +L D   + V 
Sbjct: 146 REGKFKQELAADIKGLMELYEASQLSIQEENILDEAGAFSAHFLNCWTPHLCDHQTRIVS 205

Query: 63  DALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQ--- 122
           + L +PFH+ L +  +KN+  F    Q  N++I  F +LAKLDFN +Q+++++E++Q   
Sbjct: 206 NTLKHPFHRTLARSTIKNFLHFY-NFQGENEYIQTFTELAKLDFNMIQSIHRQEINQVSN 265

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 266 WWNNLGLASELKFARDQPEKWCMWPLVGVTDPSLSWQRIELAKPVSLVYLIDDIFDLGGT 325

Query: 183 -----LFL-----WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS- 242
                LF      W++ A   LP+ MKIC+  LY+ TN++++K    H +NPIHSL+K+ 
Sbjct: 326 PDQLTLFTEAVNRWEITATEDLPYHMKICFRALYDVTNQIAYKVYKKHQYNPIHSLKKAW 385

Query: 243 ----------------------------------------HVFFLLGQGINNKTVQLLNT 302
                                                   H FFL+G GI ++T+ LLN 
Sbjct: 386 ARLCNAFLEEAKWFAAGKLPKADEYLNTAIVTSGVHLVLVHTFFLMGDGITDQTINLLNN 445

Query: 303 -NPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISD 307
            +PGI+SS A+ILRL DDLGSA+DENQ+GYDGSYIE Y K+    SV  AR  VI MISD
Sbjct: 446 DDPGIISSVATILRLWDDLGSAQDENQDGYDGSYIECYMKDFPGTSVRDARNHVISMISD 505

BLAST of HG10021127 vs. ExPASy Swiss-Prot
Match: B9RXW4 (Probable terpene synthase 13 OS=Ricinus communis OX=3988 GN=TPS13 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 4.7e-54
Identity = 134/420 (31.90%), Postives = 198/420 (47.14%), Query Frame = 0

Query: 2   EKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEK-LEKIGKTSGHILKTRIGNLDDSMAKY 61
           +KEG  ++++ +D+ GL  +YEA QL +  E+  L++ G  S  +L + +  LDDS A+ 
Sbjct: 154 DKEGNFDQKLSDDIRGLMELYEASQLSIGAEDHILDEAGDYSHQLLSSWMTRLDDSQARI 213

Query: 62  VGDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQ- 121
           + + L +P HK L +    N+ R+   +     W+   Q+LAK+DF  VQ+  Q+E+ Q 
Sbjct: 214 IKNTLDHPHHKNLARFRATNFNRYF-HMANIEGWMNELQELAKIDFQMVQSQNQQEIFQV 273

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 274 AGWWKDLGISKELKFVRNQPLKWYIWSMATLSDPSLSQQRIDLTKPISFIYIIDDIFDVQ 333

Query: 182 -------LFL-----WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK 241
                  LF      WD+ A+  LP  M+ C+  L   TNE+ +K    HGWNP+HSL++
Sbjct: 334 GSLDELTLFTEIVKRWDVEAVEQLPGYMRACFKALDSVTNEIGYKVYKQHGWNPVHSLRE 393

Query: 242 S-----------------------------------------HVFFLLGQGINNKTVQLL 301
           +                                         H+F+LLG G+  + V  +
Sbjct: 394 TWASLCKAFLVEARWFASGHLPAAEEYLQNGIVSSGVHVVLVHIFYLLGHGVTREGVDFI 453

Query: 302 NTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMIS 307
              P I++STA+ILRL DDLG +KDENQ+G+DGSY+E Y KE+    V+ A ++V  MIS
Sbjct: 454 GNRPAIITSTATILRLWDDLGISKDENQDGHDGSYVECYVKEHKGSLVEIATKKVTVMIS 513

BLAST of HG10021127 vs. ExPASy TrEMBL
Match: A0A1S4DSD7 ((3S,6E)-nerolidol synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103485748 PE=4 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 4.1e-125
Identity = 249/422 (59.00%), Postives = 275/422 (65.17%), Query Frame = 0

Query: 1   MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
           M+K GELNK+ IED+EGLTSVYEAWQLCLPGE+KL+KIG+ SGHIL+T+IGNL++ MAK+
Sbjct: 179 MDKNGELNKKGIEDIEGLTSVYEAWQLCLPGEDKLDKIGEFSGHILRTQIGNLENGMAKH 238

Query: 61  VGDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQL 120
           VGD LTNPFHK L KL++KNYF +    Q TNKWIFAFQ LAKLDFNRVQ LY+ E+SQL
Sbjct: 239 VGDTLTNPFHKSLAKLYIKNYFGY----QATNKWIFAFQNLAKLDFNRVQKLYRHEISQL 298

Query: 121 FL---------------------------------------------------------- 180
            L                                                          
Sbjct: 299 LLWWKKAGLCEELKFSRDQPLKWYICSVVCLADPKFSEERVELTKSISFIYLLDDIYDLY 358

Query: 181 ---------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK 240
                          WDL A+HSLPHPMK CYIKLYETT EL HKFSL HGWNPIHSLQK
Sbjct: 359 GSIEELRLFTQAIQRWDLEAVHSLPHPMKFCYIKLYETTKELGHKFSLKHGWNPIHSLQK 418

Query: 241 S------------------------------------------HVFFLLGQGINNKTVQL 300
           S                                          H+FFLLGQGINNKT+QL
Sbjct: 419 SVPFVTCEAFLVEAEWFASGHFPSAKEYLENGAVSSGVHVVLEHIFFLLGQGINNKTLQL 478

Query: 301 LNTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMI 307
           L+TNP  VSSTASILRLSDDLGSAKDENQEGYDGSYI+YY KEN EISVDSARQRVIHMI
Sbjct: 479 LDTNPATVSSTASILRLSDDLGSAKDENQEGYDGSYIDYYLKENPEISVDSARQRVIHMI 538

BLAST of HG10021127 vs. ExPASy TrEMBL
Match: A0A0A0LX78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G066550 PE=4 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.6e-119
Identity = 245/419 (58.47%), Postives = 268/419 (63.96%), Query Frame = 0

Query: 3   KEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYVG 62
           K GELNK+V ED+EGLTSVYEA QLCLPGEEKLE IG+  GHIL+T+I NL++ MAK+VG
Sbjct: 184 KNGELNKKVFEDIEGLTSVYEACQLCLPGEEKLEIIGEFCGHILRTQIRNLENGMAKHVG 243

Query: 63  DALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQLFL 122
           D LTNPFHK L KLF+K +F F    + TNKWIFAFQKLAKLDFNR Q  YQ E+SQL L
Sbjct: 244 DTLTNPFHKSLAKLFIKTHFGF----KATNKWIFAFQKLAKLDFNRAQKSYQHEISQLIL 303

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 304 WWKKTGLSEELKFSRHQPLKWYICSLVCLGDPKFSEERVELTKSISFIYLLDDIYDLYGS 363

Query: 183 -------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS- 242
                        W+L AI+SLPHPMK CYIKLYETTNELSHKFSL HGWNPIH LQKS 
Sbjct: 364 IEELRLFTQAIQRWNLEAINSLPHPMKFCYIKLYETTNELSHKFSLKHGWNPIHCLQKSW 423

Query: 243 ----------------------------------------HVFFLLGQGINNKTVQLLNT 302
                                                   H FFLLGQGINNKT+QLL+T
Sbjct: 424 TSLCEAFLVEAEWFASGHFPSAKEYLENGEVSSGVHVVLEHTFFLLGQGINNKTLQLLDT 483

Query: 303 NPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISDA 307
           NP IVSSTASILRLSDDLGSAKDENQEGYDGSYI+YY KEN EISVDSA+QRV HMISDA
Sbjct: 484 NPAIVSSTASILRLSDDLGSAKDENQEGYDGSYIDYYMKENPEISVDSAQQRVNHMISDA 543

BLAST of HG10021127 vs. ExPASy TrEMBL
Match: A0A6J1CEA4 ((3S,6E)-nerolidol synthase 1-like OS=Momordica charantia OX=3673 GN=LOC111010875 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 5.9e-108
Identity = 229/422 (54.27%), Postives = 260/422 (61.61%), Query Frame = 0

Query: 1   MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
           M+KEG L KEV ED+EGLT++YEA QLCLP E+KLEKIG  SGH+LK  IGNLDD  A +
Sbjct: 175 MDKEGHLGKEVREDIEGLTALYEASQLCLPQEDKLEKIGNLSGHMLKISIGNLDDHWANH 234

Query: 61  V-GDALTNPFHKCLGKLFVKNYFRFQP-QLQPTNKWIFAFQKLAKLDFNRVQNLYQREMS 120
           V  + L NPFHK L    VKNYF F+   L+ TNKWI+ FQK+AKL+FNRVQ+++Q+E+S
Sbjct: 235 VISNTLANPFHKSLANFLVKNYFGFEDYHLRATNKWIYVFQKMAKLEFNRVQSIHQQEIS 294

Query: 121 QL-----------------------------FL--------------------------- 180
           QL                             FL                           
Sbjct: 295 QLSRWWGQTGLSKELEFARDQPLKWYICSLAFLTDPIFSEERVDLAKSLSFIYLVDDIYD 354

Query: 181 -----------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSL 240
                            WDLAAI +LPH MKICYIKLYETTNE+S+KF   HGWNPI+SL
Sbjct: 355 LYGSLEQLRLFTKAIQRWDLAAIDNLPHSMKICYIKLYETTNEISNKFFQKHGWNPINSL 414

Query: 241 QKS-----------------------------------------HVFFLLGQGINNKTVQ 300
           QKS                                         H+FFLLGQGIN +T  
Sbjct: 415 QKSWATLCDAFLVEAEWFASGNLPSAKEYLRNGALSCGVHVVLAHIFFLLGQGINKQTEL 474

Query: 301 LLNTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHM 307
           LL++NPGIVSSTA+ILRL DDLGSAKDENQEGYDGSYIE Y KEN EI +DS RQRV HM
Sbjct: 475 LLDSNPGIVSSTATILRLCDDLGSAKDENQEGYDGSYIECYMKENPEICLDSTRQRVKHM 534

BLAST of HG10021127 vs. ExPASy TrEMBL
Match: A0A6J1EH51 (uncharacterized protein LOC111434068 OS=Cucurbita moschata OX=3662 GN=LOC111434068 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 1.4e-93
Identity = 206/418 (49.28%), Postives = 246/418 (58.85%), Query Frame = 0

Query: 4    EGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYVGD 63
            EG L+ +  E++EGLTS+YEA QLCLP +EKLEKIG  S  ILK  + N DD+++K+V  
Sbjct: 759  EGLLDTKFSENIEGLTSLYEASQLCLPEDEKLEKIGNFSARILKKLVRNRDDNLSKHVRK 818

Query: 64   ALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQLFL- 123
            A+ NPFHK L K  VK+YF      Q  NKWI+ FQ +AKLDFNRVQ L+  E+SQ  + 
Sbjct: 819  AMANPFHKSLVKFVVKDYF----GSQSPNKWIYVFQHMAKLDFNRVQKLHGLELSQFIMW 878

Query: 124  ------------------------------------------------------------ 183
                                                                        
Sbjct: 879  WKKTGLCEELKFARDQPLKWYICSMAFLADPMFSEERVELTKCISFIYLIDDIYDVYGSL 938

Query: 184  ------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS-- 243
                        WDLAA+  LP+PMK C IKL+ETTNE+ HKF L HGWNPI+SL+KS  
Sbjct: 939  EELRLFTKAIQRWDLAALDGLPNPMKFCIIKLHETTNEICHKFYLKHGWNPINSLRKSWV 998

Query: 244  ---------------------------------------HVFFLLGQGINNKTVQLLNTN 303
                                                   H+FFLLGQG++N+ V LL++N
Sbjct: 999  RLCEAFLVEAEWFGSSHLPSAKEYLENGEVSSGVHVVLAHIFFLLGQGVSNEAV-LLSSN 1058

Query: 304  PGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISDAW 307
            P IVSSTASILRLSDDLGSAKDENQEG+DGSYIE Y KEN  ISVDSAR+R+ H+ISDAW
Sbjct: 1059 PDIVSSTASILRLSDDLGSAKDENQEGHDGSYIECYMKENPGISVDSARKRISHVISDAW 1118

BLAST of HG10021127 vs. ExPASy TrEMBL
Match: A0A6J1KNB1 (uncharacterized protein LOC111495716 OS=Cucurbita maxima OX=3661 GN=LOC111495716 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.9e-90
Identity = 205/421 (48.69%), Postives = 241/421 (57.24%), Query Frame = 0

Query: 1    MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
            ++ EG L+ +  E++EGLTS+YEA QLCLP +EKLEKIG  S  ILK    N DD++ K+
Sbjct: 798  IDHEGLLDTKFRENIEGLTSLYEASQLCLPEDEKLEKIGNFSACILKKLARNHDDNLGKH 857

Query: 61   VGDALTNPFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQL 120
            V  A+ NPFHK L K  VK+YF      Q  NKWI+ FQ +AKLDFNRVQ L+  E+SQ 
Sbjct: 858  VRKAVANPFHKRLVKFVVKDYF----GSQSPNKWIYVFQHMAKLDFNRVQKLHGLELSQF 917

Query: 121  FL---------------------------------------------------------- 180
             +                                                          
Sbjct: 918  IMWWKRTGLCEELKFARDQPLKWYICSMAFLADPMFSEERVELTKCISFIYLIDDIYDVY 977

Query: 181  ---------------WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK 240
                           WDLAA+  LP PMK C IKL+ETTNE+ HKF L HGWNPI+SL K
Sbjct: 978  GSLEELRLFTKAIQRWDLAALDGLPKPMKFCIIKLHETTNEICHKFYLKHGWNPINSLCK 1037

Query: 241  S-----------------------------------------HVFFLLGQGINNKTVQLL 300
            S                                         H+FFLLGQG++N+ V LL
Sbjct: 1038 SWVRLCEAFLVEAEWFGSSHLPSAKEYLENGEVSSGVHVVLAHIFFLLGQGVSNEAV-LL 1097

Query: 301  NTNPGIVSSTASILRLSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMIS 307
            + NP IVSSTASILRLSDDLGSAKDENQ+G+DGSYIE Y KEN  ISVDS R+ + HMIS
Sbjct: 1098 SCNPDIVSSTASILRLSDDLGSAKDENQKGHDGSYIECYMKENPGISVDSTREHISHMIS 1157

BLAST of HG10021127 vs. TAIR 10
Match: AT1G61680.1 (terpene synthase 14 )

HSP 1 Score: 170.6 bits (431), Expect = 1.9e-42
Identity = 123/417 (29.50%), Postives = 185/417 (44.36%), Query Frame = 0

Query: 1   MEKEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKY 60
           ++K+G     V  D++GLT ++EA +L + GEE L+   + +   L       +    + 
Sbjct: 157 LDKKGGFKDVVKNDVKGLTELFEASELRVEGEETLDGAREFTYSRLNELCSGRESHQKQE 216

Query: 61  VGDALTNPFHKCLGKLFVKNY---FRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREM 120
           +  +L  P HK +  L  K +    +   Q  P  +W+ +  ++A++D  R+++L Q EM
Sbjct: 217 IMKSLAQPRHKTVRGLTSKRFTSMIKIAGQEDP--EWLQSLLRVAEIDSIRLKSLTQGEM 276

Query: 121 SQLF-------------------------------------------------------- 180
           SQ F                                                        
Sbjct: 277 SQTFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYVIDDIF 336

Query: 181 -----------------LWDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHS 240
                             WD   + +LP  M++C+  L   T E+S K   +HGWNP ++
Sbjct: 337 DVYGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGWNPTYA 396

Query: 241 LQKS-----------------------------------------HVFFLLGQGINNKTV 300
           L++S                                         H + LLG+ +  + V
Sbjct: 397 LRQSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEELTKEKV 456

BLAST of HG10021127 vs. TAIR 10
Match: AT1G61680.2 (terpene synthase 14 )

HSP 1 Score: 169.5 bits (428), Expect = 4.3e-42
Identity = 123/415 (29.64%), Postives = 183/415 (44.10%), Query Frame = 0

Query: 3   KEGELNKEVIEDMEGLTSVYEAWQLCLPGEEKLEKIGKTSGHILKTRIGNLDDSMAKYVG 62
           K+G     V  D++GLT ++EA +L + GEE L+   + +   L       +    + + 
Sbjct: 152 KKGGFKDVVKNDVKGLTELFEASELRVEGEETLDGAREFTYSRLNELCSGRESHQKQEIM 211

Query: 63  DALTNPFHKCLGKLFVKNY---FRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMSQ 122
            +L  P HK +  L  K +    +   Q  P  +W+ +  ++A++D  R+++L Q EMSQ
Sbjct: 212 KSLAQPRHKTVRGLTSKRFTSMIKIAGQEDP--EWLQSLLRVAEIDSIRLKSLTQGEMSQ 271

Query: 123 LF---------------------------------------------------------- 182
            F                                                          
Sbjct: 272 TFKWWTELGLEKDVEKARSQPLKWHTWSMKILQDPTLTEQRLDLTKPISLVYVIDDIFDV 331

Query: 183 ---------------LWDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQ 242
                           WD   + +LP  M++C+  L   T E+S K   +HGWNP ++L+
Sbjct: 332 YGELEELTIFTRVVERWDHKGLKTLPKYMRVCFEALDMITTEISMKIYKSHGWNPTYALR 391

Query: 243 KS-----------------------------------------HVFFLLGQGINNKTVQL 301
           +S                                         H + LLG+ +  + V+L
Sbjct: 392 QSWASLCKAFLVEAKWFNSGYLPTTEEYMKNGVVSSGVHLVMLHAYILLGEELTKEKVEL 451

BLAST of HG10021127 vs. TAIR 10
Match: AT4G16730.1 (terpene synthase 02 )

HSP 1 Score: 68.9 bits (167), Expect = 7.8e-12
Identity = 54/230 (23.48%), Postives = 102/230 (44.35%), Query Frame = 0

Query: 123 WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQK--------------- 182
           WD+  +  LP+ M++C++ LY   N++ +    + G N I  L++               
Sbjct: 311 WDVNHVERLPNYMRMCFLFLYNEINQIGYDVLRDKGLNVIPYLKQVWTDLFKTFLTESKW 370

Query: 183 --------------------------SHVFFLLGQGINNKTVQLLNTNPGIVSSTASILR 242
                                      H+F +L   I+++T+   + N  +V S A+ILR
Sbjct: 371 YKTGHKPSFEEYMQNGVISSSVPTILLHLFSVLSDHISDQTLTDDSKNHSVVRSCATILR 430

Query: 243 LSDDLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISDAWKRLNRESQS--R 302
           L++DL ++ +E   G     ++ Y  E    S + AR+ +  MISD+W  +N + ++   
Sbjct: 431 LANDLATSTEEMARGDSPKSVQCYMYET-RASEEEARRHMQSMISDSWDIINSDLKTAHT 490

Query: 303 NPFPATFIQASQNIARFVPLLYGYDENQNLPTLEKLV---KSMLYESVQI 307
           +  P  F+ A+ N+ R V  +Y + +    P   K V   +S+L+  V +
Sbjct: 491 SSLPRGFLAAAANLNRVVQCIYRHGDGHGSPEKTKTVDYIQSVLFNPVPL 539

BLAST of HG10021127 vs. TAIR 10
Match: AT4G16740.1 (terpene synthase 03 )

HSP 1 Score: 65.5 bits (158), Expect = 8.7e-11
Identity = 88/404 (21.78%), Postives = 153/404 (37.87%), Query Frame = 0

Query: 13  EDMEGLTSVYEAWQLCLPGEEKL-EKIGKTSGHILKTRIGNLDD----SMAKYVGDALTN 72
           +D++G+ S+YEA  L    + KL E I  T+  + K    N ++    ++ + V  AL  
Sbjct: 157 KDIKGILSLYEASYLSTRIDTKLKESIYYTTKRLRKFVEVNKNETKSYTLRRMVIHALEM 216

Query: 73  PFHKCLGKLFVKNYFRFQPQLQPTNKWIFAFQKLAKLDFNRVQNLYQREMS--------- 132
           P+H+ +G+L  + Y     +    N  +    +LAKLDFN VQ ++Q E+          
Sbjct: 217 PYHRRVGRLEARWYIEVYGERHDMNPILL---ELAKLDFNFVQAIHQDELKSLSSWWSKT 276

Query: 133 -----------------------------------------------------------Q 192
                                                                      Q
Sbjct: 277 GLTKHLDFVRDRITEGYFSSVGVMYEPEFAYHRQMLTKVFMLITTIDDIYDIYGTLEELQ 336

Query: 193 LFL-----WDLAAIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS------ 252
           LF      WD+  +  LP+ MK+C++ L    N++ +    + G+N I  L++S      
Sbjct: 337 LFTTIVEKWDVNRLEELPNYMKLCFLCLVNEINQIGYFVLRDKGFNVIPYLKESWADMCT 396

Query: 253 -----------------------------------HVFFLLGQGINNKTVQLLNT-NPGI 297
                                              H+F LL    +++T+ +L + N  +
Sbjct: 397 TFLKEAKWYKSGYKPNFEEYMQNGWISSSVPTILLHLFCLL----SDQTLDILGSYNHSV 456

BLAST of HG10021127 vs. TAIR 10
Match: AT2G24210.1 (terpene synthase 10 )

HSP 1 Score: 58.5 bits (140), Expect = 1.1e-08
Identity = 61/278 (21.94%), Postives = 113/278 (40.65%), Query Frame = 0

Query: 78  VKNYFRFQPQLQPTNKWIFAFQKLAKLD--FNRVQNLYQ----REMSQLFL-----WDLA 137
           V+NYF    Q+Q   ++ +  Q + K++     + ++Y      E  QLF      WD+ 
Sbjct: 306 VENYFWTIGQIQEP-QYGYVRQTMTKINALLTTIDDIYDIYGTLEELQLFTVAFENWDIN 365

Query: 138 AIHSLPHPMKICYIKLYETTNELSHKFSLNHGWNPIHSLQKS------------------ 197
            +  LP  M++C++ +Y   N ++ +       N I  L+KS                  
Sbjct: 366 RLDELPEYMRLCFLVIYNEVNSIACEILRTKNINVIPFLKKSWTDVSKAYLVEAKWYKSG 425

Query: 198 -----------------------HVFFLLGQGINNKTVQLLNTN-PGIVSSTASILRLSD 257
                                  H + +    ++ + ++ L+ +   +V  ++S+ RL++
Sbjct: 426 HKPNLEEYMQNARISISSPTIFVHFYCVFSDQLSIQVLETLSQHQQNVVRCSSSVFRLAN 485

Query: 258 DLGSAKDENQEGYDGSYIEYYTKENLEISVDSARQRVIHMISDAWKRLNRE--SQSRNPF 301
           DL ++ DE   G     I+ Y  E    S D AR  V  MI+D W  +N E  + S +  
Sbjct: 486 DLVTSPDELARGDVCKSIQCYMSET-GASEDKARSHVRQMINDLWDEMNYEKMAHSSSIL 545