HG10021059 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10021059 vs. NCBI nr
Match: XP_038895143.1 (glucan endo-1,3-beta-glucosidase 1 [Benincasa hispida])

HSP 1 Score: 796.6 bits (2056), Expect = 1.3e-226
Identity = 429/565 (75.93%), Postives = 443/565 (78.41%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKFPPLIKG+KV+KDDKQPF+GV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFPPLIKGEKVQKDDKQPFIGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKALSGSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALSGSKIRVIISVPNNQLLAIGFSNTTAAAWIDRNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQITVSTPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITVSTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDV+VLVTESGWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVMVLVTESGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHILDRSGTPFHPE+TSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHILDRSGTPFHPEITSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 P+SEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY
Sbjct: 361 ----------------------PLSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 480
           CIAMDEFDAKTLQTALDWACGPGKANCTEI PGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEILPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

Query: 481 AGSCDFKGVAMITTTDPSHGSCIFPGSKKVRNNTKETVNSTQIPEASGGAAQRLKLMGEM 506
           AGSCDFKG+AMITTTDPSHGSCIFPGSKKV+NNTKE VNSTQIP+ASG AAQRLKL   M
Sbjct: 481 AGSCDFKGLAMITTTDPSHGSCIFPGSKKVKNNTKERVNSTQIPQASGVAAQRLKL---M 520

BLAST of HG10021059 vs. NCBI nr
Match: XP_004145115.1 (glucan endo-1,3-beta-glucosidase 1 [Cucumis sativus] >KGN64511.1 hypothetical protein Csa_013695 [Cucumis sativus])

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-219
Identity = 408/536 (76.12%), Postives = 421/536 (78.54%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKFPPLIKG KV+KDDKQPFVGV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFPPLIKGVKVDKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWISRNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDVVVLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. NCBI nr
Match: XP_022139909.1 (glucan endo-1,3-beta-glucosidase 1 [Momordica charantia] >XP_022139910.1 glucan endo-1,3-beta-glucosidase 1 [Momordica charantia])

HSP 1 Score: 772.7 bits (1994), Expect = 2.0e-219
Identity = 415/563 (73.71%), Postives = 436/563 (77.44%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKF-PPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDL 60
           MA+SK  LFFLF FVFLTASEGKF  PLIKGQK+EK+D+QPFVGV I  DVSNLLSPSDL
Sbjct: 1   MAESKFHLFFLFFFVFLTASEGKFQKPLIKGQKLEKEDRQPFVGVNIGSDVSNLLSPSDL 60

Query: 61  VSFLQFQKITHIRLYDTNPDILKALSGSKIR----------------------------- 120
           VSFL+FQKITHIRLYD NP+ILKALSG+KIR                             
Sbjct: 61  VSFLRFQKITHIRLYDANPEILKALSGTKIRVIISVPNNQLIAIGSSNATAAAWIGRNVV 120

Query: 121 -----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIIL 180
                TLISGISVGDEVL TIPSASPLLLPAIESLYNALV+ANLQTQI +STPHAASIIL
Sbjct: 121 AYYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVSANLQTQIPISTPHAASIIL 180

Query: 181 DPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLT 240
           DPFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDN+LFKPLT
Sbjct: 181 DPFPPSQAFFNQSLVQFMLPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNSLFKPLT 240

Query: 241 PSKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASID 300
           PSKEM                         NLNITDVVVLVTESGWPSKGDSKEPYA+ID
Sbjct: 241 PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYATID 300

Query: 301 NADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVN 360
           NADTFNSNLIKHILDRSGTPFHPE+TSSVYIYELFNEDLRSP                  
Sbjct: 301 NADTFNSNLIKHILDRSGTPFHPEITSSVYIYELFNEDLRSP------------------ 360

Query: 361 LLHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT 420
                                  PVSEANWGLFYGNSTPVYLLHV+GSGTFLANDTTNQT
Sbjct: 361 -----------------------PVSEANWGLFYGNSTPVYLLHVTGSGTFLANDTTNQT 420

Query: 421 YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGK 480
           YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGK
Sbjct: 421 YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEPCYQPNNVKNHASYAFDSYYQKEGK 480

Query: 481 TAGSCDFKGVAMITTTDPSHGSCIFPGSKKVRNNTKETVNSTQIPEASGGAAQRLKLMGE 503
           T+GSCDFKGVAMITTTDPSHGSCIFPGSKKV N TKETVNSTQ P+ASG AA RL+L GE
Sbjct: 481 TSGSCDFKGVAMITTTDPSHGSCIFPGSKKVSNKTKETVNSTQTPQASGAAAHRLRLFGE 520

BLAST of HG10021059 vs. NCBI nr
Match: XP_008440956.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 1 [Cucumis melo] >XP_008440965.1 PREDICTED: glucan endo-1,3-beta-glucosidase 1 [Cucumis melo] >ADN34059.1 glucan endo-13-beta-glucosidase [Cucumis melo subsp. melo] >KAA0056847.1 glucan endo-1,3-beta-glucosidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 768.5 bits (1983), Expect = 3.7e-218
Identity = 405/536 (75.56%), Postives = 419/536 (78.17%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT+
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTF 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. NCBI nr
Match: TYJ99350.1 (glucan endo-1,3-beta-glucosidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 767.7 bits (1981), Expect = 6.3e-218
Identity = 405/536 (75.56%), Postives = 419/536 (78.17%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKF PLI G KVEKDDKQPFVGV I  DVS LLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSKLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT+
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTF 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. ExPASy Swiss-Prot
Match: O65399 (Glucan endo-1,3-beta-glucosidase 1 OS=Arabidopsis thaliana OX=3702 GN=At1g11820 PE=2 SV=3)

HSP 1 Score: 613.6 bits (1581), Expect = 2.0e-174
Identity = 327/537 (60.89%), Postives = 376/537 (70.02%), Query Frame = 0

Query: 8   LFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFLQFQK 67
           LFF F  + ++A+      L    KV++ DK PFVG  I  DVSNLLSP++LV FLQ QK
Sbjct: 13  LFFFFTLLLISANSSS---LSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQK 72

Query: 68  ITHIRLYDTNPDILKALSGSKIR----------------------------------TLI 127
           + H+RLYD +P++LKAL+ +K+R                                  TLI
Sbjct: 73  VNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLI 132

Query: 128 SGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQA 187
           + ISVGDEVL T+PS++PLLLPAIESLYNALVA+NL TQI VSTPHAASI+LD FPPSQA
Sbjct: 133 TAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQA 192

Query: 188 FFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEM--- 247
           +FNQ+    ++PLLQFLSKTGSPLMMN YPYYV+MQNKGVVPLDN LF+PLTPSKEM   
Sbjct: 193 YFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDP 252

Query: 248 ----------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSN 307
                                 NLN++DV VLVTESGWPSKGDSKEPYA+IDNADT+NSN
Sbjct: 253 NTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSN 312

Query: 308 LIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHVSGSG 367
           LIKH+ DR+GTP HPE+TSSVYIYELFNEDLR+P                          
Sbjct: 313 LIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAP-------------------------- 372

Query: 368 TWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDEF 427
                          PVSEA+WGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMD  
Sbjct: 373 ---------------PVSEASWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDGV 432

Query: 428 DAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGSCDFK 485
           DAKTLQ ALDWACGPG++NC+EIQPGE+CYQPNNVK HAS+AF+SYYQKEG+ +GSCDFK
Sbjct: 433 DAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFK 492

BLAST of HG10021059 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 3.6e-107
Identity = 219/518 (42.28%), Postives = 301/518 (58.11%), Query Frame = 0

Query: 4   SKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFL 63
           S L L  L L + + AS    PP          D+  ++GV I  D+S++  P+ +V+ L
Sbjct: 3   SLLHLLLLSLSLLVLASASPSPPA---------DEGSYIGVNIGTDLSDMPHPTQVVALL 62

Query: 64  QFQKITHIRLYDTNPDILKALSGSKIR--------------------------------- 123
           + Q+I HIRLY+ +P +L AL+ + I+                                 
Sbjct: 63  KAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYP 122

Query: 124 -TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFP 183
            T+I+ +SVG EVL ++ +A+P+L+ AI++++ AL++ANL   I VSTP + S+ILDPFP
Sbjct: 123 ATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFP 182

Query: 184 PSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKE 243
           PSQAFFN+SL   ++PLL FL  T S LM+N YPY  +MQ+ GV+PLD ALFKP+ P+KE
Sbjct: 183 PSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKE 242

Query: 244 -------------------------MNLNITDVVVLVTESGWPSKGDSKEPYASIDNADT 303
                                      LN T++ VLVTESGWPSKG++ EP A++DNA+T
Sbjct: 243 AVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANT 302

Query: 304 FNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHV 363
           +NSNLI+H+L+++GTP  P +  S YIYEL+NED +                        
Sbjct: 303 YNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTK------------------------ 362

Query: 364 SGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIA 423
                             A +SE NWGLF  N  PVY+L ++ SG+ LANDTTNQTYC A
Sbjct: 363 ------------------AGLSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTA 422

Query: 424 MDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGS 461
            +  D K LQ ALDWACGPGK +C+ I+ GE CY+P+NV  HA+YAFD+YY + G    +
Sbjct: 423 REGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDA 469

BLAST of HG10021059 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 388.7 bits (997), Expect = 1.0e-106
Identity = 216/517 (41.78%), Postives = 300/517 (58.03%), Query Frame = 0

Query: 11  LFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFLQFQKITH 70
           L LF+FL AS                 +   +GV I  +V+N+ SP+ +V+ L+ Q I  
Sbjct: 5   LLLFLFLFASSAL-------------SQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINR 64

Query: 71  IRLYDTNPDILKALSGSKIRTLIS----------------------------------GI 130
           +RLYD +  +L A + + ++ +IS                                   I
Sbjct: 65  VRLYDADRSMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTI 124

Query: 131 SVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQAFFN 190
           +VG EVL ++ +A+ +L+ A++ +  ALV ANL  QI VSTPH+++IILD FPPSQAFFN
Sbjct: 125 AVGSEVLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN 184

Query: 191 QSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKE------- 250
           ++    ++PLL+FL  TGSPL++N YPY+ ++Q+ GV+PLD ALF+PL  +KE       
Sbjct: 185 KTWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTL 244

Query: 251 ------------------MNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSNLIK 310
                               LN T++ ++VTESGWPSKG   E  A+++NA+T+NSNLI+
Sbjct: 245 LHYTNVFDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQ 304

Query: 311 HILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHVSGSGTWA 370
           H+++++GTP HP    + YIYEL+NED R                               
Sbjct: 305 HVINKTGTPKHPGTAVTTYIYELYNEDTR------------------------------- 364

Query: 371 CGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDEFDAK 430
             PG        PVSE NWGLFY N TPVY L ++G+G  LANDTTNQT+CIA ++ D K
Sbjct: 365 --PG--------PVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKEKVDRK 424

Query: 431 TLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGSCDFKGVA 468
            LQ ALDWACGPGK +C+ +  GE+CY+P++V  H++YAF++YYQK GK +GSCDFKGVA
Sbjct: 425 MLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVA 466

BLAST of HG10021059 vs. ExPASy Swiss-Prot
Match: Q94CD8 (Glucan endo-1,3-beta-glucosidase 4 OS=Arabidopsis thaliana OX=3702 GN=At3g13560 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.8e-85
Identity = 187/491 (38.09%), Postives = 257/491 (52.34%), Query Frame = 0

Query: 41  FVGV-IWEDVSNLLSPSDLVSFLQFQKITHIRLYDTNPDILKALSGSKIR---------- 100
           F+GV I  D++N+  PSD+V+ L+ Q+ITH+RLYD N  +LKA + + I           
Sbjct: 25  FIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEI 84

Query: 101 ------------------------TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVA 160
                                   T I+ I+VG EVL TIP  +P+L  A+ +++ ALVA
Sbjct: 85  LKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVA 144

Query: 161 ANLQTQITVSTPHAASIILDPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYV 220
           +NL  ++ VS+P +  I+  PFPPS + F+ S    V  LLQFL  TGS  M+N YPYY 
Sbjct: 145 SNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYG 204

Query: 221 FMQNKGVVPLDNALFKPLTPSKEM-------------------------NLNITDVVVLV 280
           +    G+ PLD ALFK L+P K++                          LN + + V+V
Sbjct: 205 YTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVV 264

Query: 281 TESGWPSKGDSKEPYASIDNADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRS 340
           TE+GWPS G S E  A++ NA+TFN+NLIK +L+ SG P  P++  + YIYEL+NED RS
Sbjct: 265 TETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRS 324

Query: 341 PPVSEANWGLFYGNSTPVNLLHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVY 400
            PVSE NWG+ + N T V  L +SG  + A                              
Sbjct: 325 GPVSERNWGILFPNGTSVYPLSLSGGSSSAA----------------------------- 384

Query: 401 LLHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPN 460
              ++GS  F          C+A  + D   L   L+WACG G+ANC  IQPG+ CY PN
Sbjct: 385 ---LNGSSMF----------CVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPN 444

Query: 461 NVKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSHGSCIFPGSKKVRNNTKETVNS 472
           +VK+HAS+AF+ YYQK     G+CDF G A+ TT DPS+ +C + GS           N 
Sbjct: 445 DVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPSYRTCAYTGSLNA-----NATNG 468

BLAST of HG10021059 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.7e-69
Identity = 157/460 (34.13%), Postives = 238/460 (51.74%), Query Frame = 0

Query: 42  VGVIW-EDVSNLLSPSDLVSFLQFQKITHIRLYDTNPDILKALSGSKIR----------- 101
           +G+ +  +  NL SP+ +   +Q   I  +R+YD N D+LKA + + I            
Sbjct: 27  IGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNADLL 86

Query: 102 -----------------------TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAA 161
                                  T I+ ISVG EV     +A+ L+LPA+ +++ AL  +
Sbjct: 87  AFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALKKS 146

Query: 162 NLQTQITVSTPHAASIILDPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVF 221
            L  +I +S+ H+ +I+   FPPS A F++    F+ P+L+FL +  SP M++ YPYY +
Sbjct: 147 GLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYYAY 206

Query: 222 MQNKGVVPLDNALFK-------PLTPSKEMN---------------LNITDVVVLVTESG 281
             +   VPL+ ALF+       P T     N               ++   V V+VTESG
Sbjct: 207 RDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTESG 266

Query: 282 WPSKGDSKEPYASIDNADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVS 341
           WPSKG  KE  A+ +NA  +N+NLI+H++   GTP  P     VY++ LFNE+ +    S
Sbjct: 267 WPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGIES 326

Query: 342 EANWGLFYGNSTPVNLLHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHV 401
           E NWG+FY N T V  L  +G  T    P           S ++  +  GNST V +   
Sbjct: 327 ERNWGMFYANGTNVYALDFTGENTTPVSPTN--STTGTSPSPSSSPIINGNST-VTIGGG 386

Query: 402 SGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKN 445
            G GT        + +CIA  +     LQTALDWACGPG  +C+ +QP + C++P+ V +
Sbjct: 387 GGGGT--------KKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLS 446

BLAST of HG10021059 vs. ExPASy TrEMBL
Match: A0A0A0LX25 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G062370 PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 7.2e-220
Identity = 408/536 (76.12%), Postives = 421/536 (78.54%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLFVFLT SEGKFPPLIKG KV+KDDKQPFVGV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFVFLTVSEGKFPPLIKGVKVDKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWISRNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDVVVLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. ExPASy TrEMBL
Match: A0A6J1CDL3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111010711 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 9.4e-220
Identity = 415/563 (73.71%), Postives = 436/563 (77.44%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKF-PPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDL 60
           MA+SK  LFFLF FVFLTASEGKF  PLIKGQK+EK+D+QPFVGV I  DVSNLLSPSDL
Sbjct: 1   MAESKFHLFFLFFFVFLTASEGKFQKPLIKGQKLEKEDRQPFVGVNIGSDVSNLLSPSDL 60

Query: 61  VSFLQFQKITHIRLYDTNPDILKALSGSKIR----------------------------- 120
           VSFL+FQKITHIRLYD NP+ILKALSG+KIR                             
Sbjct: 61  VSFLRFQKITHIRLYDANPEILKALSGTKIRVIISVPNNQLIAIGSSNATAAAWIGRNVV 120

Query: 121 -----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIIL 180
                TLISGISVGDEVL TIPSASPLLLPAIESLYNALV+ANLQTQI +STPHAASIIL
Sbjct: 121 AYYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVSANLQTQIPISTPHAASIIL 180

Query: 181 DPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLT 240
           DPFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDN+LFKPLT
Sbjct: 181 DPFPPSQAFFNQSLVQFMLPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNSLFKPLT 240

Query: 241 PSKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASID 300
           PSKEM                         NLNITDVVVLVTESGWPSKGDSKEPYA+ID
Sbjct: 241 PSKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVVVLVTESGWPSKGDSKEPYATID 300

Query: 301 NADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVN 360
           NADTFNSNLIKHILDRSGTPFHPE+TSSVYIYELFNEDLRSP                  
Sbjct: 301 NADTFNSNLIKHILDRSGTPFHPEITSSVYIYELFNEDLRSP------------------ 360

Query: 361 LLHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT 420
                                  PVSEANWGLFYGNSTPVYLLHV+GSGTFLANDTTNQT
Sbjct: 361 -----------------------PVSEANWGLFYGNSTPVYLLHVTGSGTFLANDTTNQT 420

Query: 421 YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGK 480
           YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGK
Sbjct: 421 YCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEPCYQPNNVKNHASYAFDSYYQKEGK 480

Query: 481 TAGSCDFKGVAMITTTDPSHGSCIFPGSKKVRNNTKETVNSTQIPEASGGAAQRLKLMGE 503
           T+GSCDFKGVAMITTTDPSHGSCIFPGSKKV N TKETVNSTQ P+ASG AA RL+L GE
Sbjct: 481 TSGSCDFKGVAMITTTDPSHGSCIFPGSKKVSNKTKETVNSTQTPQASGAAAHRLRLFGE 520

BLAST of HG10021059 vs. ExPASy TrEMBL
Match: A0A1S3B1W0 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103485208 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.8e-218
Identity = 405/536 (75.56%), Postives = 419/536 (78.17%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT+
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTF 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. ExPASy TrEMBL
Match: A0A5A7UQD8 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold96G00150 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.8e-218
Identity = 405/536 (75.56%), Postives = 419/536 (78.17%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT+
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTF 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. ExPASy TrEMBL
Match: E5GC60 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 1.8e-218
Identity = 405/536 (75.56%), Postives = 419/536 (78.17%), Query Frame = 0

Query: 1   MADSKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLV 60
           MADSK SLFFLFLF FLT SEGKF PLI G KVEKDDKQPFVGV I  DVSNLLSPSDLV
Sbjct: 1   MADSKFSLFFLFLFAFLTVSEGKFSPLINGVKVEKDDKQPFVGVNIGSDVSNLLSPSDLV 60

Query: 61  SFLQFQKITHIRLYDTNPDILKALSGSKIR------------------------------ 120
           SFL FQKITHIRLYDTNPDILKAL GSKIR                              
Sbjct: 61  SFLHFQKITHIRLYDTNPDILKALKGSKIRVVISVPNNQLLAIGYSNTTAAAWIARNVVA 120

Query: 121 ----TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILD 180
               TLISGISVGDEVL TIPSASPLLLPAIESLYNALVAANL TQIT+STPHAASIILD
Sbjct: 121 YYPQTLISGISVGDEVLTTIPSASPLLLPAIESLYNALVAANLHTQITISTPHAASIILD 180

Query: 181 PFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTP 240
           PFPPSQAFFNQSLVQF+LPLLQFLS+TGSPLMMNFYPYYVFMQNKGVVPLDNALFKPL P
Sbjct: 181 PFPPSQAFFNQSLVQFILPLLQFLSRTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLVP 240

Query: 241 SKEM-------------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDN 300
           SKEM                         NLNITDV+VLVTE+GWPSKGDSKEPYA+I+N
Sbjct: 241 SKEMVDPNTLLHYTNVLDAMIDSAYFSMKNLNITDVIVLVTETGWPSKGDSKEPYATINN 300

Query: 301 ADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNL 360
           AD FNSNLIKHI+DRSGTPFHPEVTSSVYIYELFNEDLRSP                   
Sbjct: 301 ADNFNSNLIKHIIDRSGTPFHPEVTSSVYIYELFNEDLRSP------------------- 360

Query: 361 LHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTY 420
                                 PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQT+
Sbjct: 361 ----------------------PVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTF 420

Query: 421 CIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKT 477
           CIA+DEFD KTLQTALDWACGPGKANCTEIQPGE CYQPNNVKNHASYAFDSYYQKEGKT
Sbjct: 421 CIAIDEFDVKTLQTALDWACGPGKANCTEIQPGEVCYQPNNVKNHASYAFDSYYQKEGKT 480

BLAST of HG10021059 vs. TAIR 10
Match: AT1G11820.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 613.6 bits (1581), Expect = 1.4e-175
Identity = 327/537 (60.89%), Postives = 376/537 (70.02%), Query Frame = 0

Query: 8   LFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFLQFQK 67
           LFF F  + ++A+      L    KV++ DK PFVG  I  DVSNLLSP++LV FLQ QK
Sbjct: 13  LFFFFTLLLISANSSS---LSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQK 72

Query: 68  ITHIRLYDTNPDILKALSGSKIR----------------------------------TLI 127
           + H+RLYD +P++LKAL+ +K+R                                  TLI
Sbjct: 73  VNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLI 132

Query: 128 SGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQA 187
           + ISVGDEVL T+PS++PLLLPAIESLYNALVA+NL TQI VSTPHAASI+LD FPPSQA
Sbjct: 133 TAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQA 192

Query: 188 FFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEM--- 247
           +FNQ+    ++PLLQFLSKTGSPLMMN YPYYV+MQNKGVVPLDN LF+PLTPSKEM   
Sbjct: 193 YFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDP 252

Query: 248 ----------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSN 307
                                 NLN++DV VLVTESGWPSKGDSKEPYA+IDNADT+NSN
Sbjct: 253 NTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSN 312

Query: 308 LIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHVSGSG 367
           LIKH+ DR+GTP HPE+TSSVYIYELFNEDLR+P                          
Sbjct: 313 LIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAP-------------------------- 372

Query: 368 TWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDEF 427
                          PVSEA+WGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMD  
Sbjct: 373 ---------------PVSEASWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDGV 432

Query: 428 DAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGSCDFK 485
           DAKTLQ ALDWACGPG++NC+EIQPGE+CYQPNNVK HAS+AF+SYYQKEG+ +GSCDFK
Sbjct: 433 DAKTLQAALDWACGPGRSNCSEIQPGESCYQPNNVKGHASFAFNSYYQKEGRASGSCDFK 492

BLAST of HG10021059 vs. TAIR 10
Match: AT1G11820.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 424.9 bits (1091), Expect = 9.3e-119
Identity = 229/370 (61.89%), Postives = 266/370 (71.89%), Query Frame = 0

Query: 8   LFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFLQFQK 67
           LFF F  + ++A+      L    KV++ DK PFVG  I  DVSNLLSP++LV FLQ QK
Sbjct: 13  LFFFFTLLLISANSSS---LSHNIKVQEQDKDPFVGFNIGTDVSNLLSPTELVKFLQAQK 72

Query: 68  ITHIRLYDTNPDILKALSGSKIR----------------------------------TLI 127
           + H+RLYD +P++LKAL+ +K+R                                  TLI
Sbjct: 73  VNHVRLYDADPELLKALAKTKVRVIISVPNNQLLAIGSSNSTAASWIGRNVVAYYPETLI 132

Query: 128 SGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQA 187
           + ISVGDEVL T+PS++PLLLPAIESLYNALVA+NL TQI VSTPHAASI+LD FPPSQA
Sbjct: 133 TAISVGDEVLTTVPSSAPLLLPAIESLYNALVASNLHTQIKVSTPHAASIMLDTFPPSQA 192

Query: 188 FFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKEM--- 247
           +FNQ+    ++PLLQFLSKTGSPLMMN YPYYV+MQNKGVVPLDN LF+PLTPSKEM   
Sbjct: 193 YFNQTWHSIMVPLLQFLSKTGSPLMMNLYPYYVYMQNKGVVPLDNCLFEPLTPSKEMVDP 252

Query: 248 ----------------------NLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSN 307
                                 NLN++DV VLVTESGWPSKGDSKEPYA+IDNADT+NSN
Sbjct: 253 NTLLHYTNVLDAMVDAAYVSMKNLNVSDVAVLVTESGWPSKGDSKEPYATIDNADTYNSN 312

Query: 308 LIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHVSGSG 316
           LIKH+ DR+GTP HPE+TSSVYIYELFNEDLR+PPVSEA+WGLFYGNSTPV LLHVSGSG
Sbjct: 313 LIKHVFDRTGTPLHPEMTSSVYIYELFNEDLRAPPVSEASWGLFYGNSTPVYLLHVSGSG 372

BLAST of HG10021059 vs. TAIR 10
Match: AT1G66250.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 390.2 bits (1001), Expect = 2.5e-108
Identity = 219/518 (42.28%), Postives = 301/518 (58.11%), Query Frame = 0

Query: 4   SKLSLFFLFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFL 63
           S L L  L L + + AS    PP          D+  ++GV I  D+S++  P+ +V+ L
Sbjct: 3   SLLHLLLLSLSLLVLASASPSPPA---------DEGSYIGVNIGTDLSDMPHPTQVVALL 62

Query: 64  QFQKITHIRLYDTNPDILKALSGSKIR--------------------------------- 123
           + Q+I HIRLY+ +P +L AL+ + I+                                 
Sbjct: 63  KAQEIRHIRLYNADPGLLIALANTGIKVIISIPNDQLLGIGQSNSTAANWVKRNVIAHYP 122

Query: 124 -TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFP 183
            T+I+ +SVG EVL ++ +A+P+L+ AI++++ AL++ANL   I VSTP + S+ILDPFP
Sbjct: 123 ATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAALLSANLDKLIKVSTPLSTSLILDPFP 182

Query: 184 PSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKE 243
           PSQAFFN+SL   ++PLL FL  T S LM+N YPY  +MQ+ GV+PLD ALFKP+ P+KE
Sbjct: 183 PSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYPYIDYMQSNGVIPLDYALFKPIPPNKE 242

Query: 244 -------------------------MNLNITDVVVLVTESGWPSKGDSKEPYASIDNADT 303
                                      LN T++ VLVTESGWPSKG++ EP A++DNA+T
Sbjct: 243 AVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIPVLVTESGWPSKGETNEPDATLDNANT 302

Query: 304 FNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHV 363
           +NSNLI+H+L+++GTP  P +  S YIYEL+NED +                        
Sbjct: 303 YNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNEDTK------------------------ 362

Query: 364 SGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIA 423
                             A +SE NWGLF  N  PVY+L ++ SG+ LANDTTNQTYC A
Sbjct: 363 ------------------AGLSEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQTYCTA 422

Query: 424 MDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGS 461
            +  D K LQ ALDWACGPGK +C+ I+ GE CY+P+NV  HA+YAFD+YY + G    +
Sbjct: 423 REGADTKMLQAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDA 469

BLAST of HG10021059 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 388.7 bits (997), Expect = 7.4e-108
Identity = 216/517 (41.78%), Postives = 300/517 (58.03%), Query Frame = 0

Query: 11  LFLFVFLTASEGKFPPLIKGQKVEKDDKQPFVGV-IWEDVSNLLSPSDLVSFLQFQKITH 70
           L LF+FL AS                 +   +GV I  +V+N+ SP+ +V+ L+ Q I  
Sbjct: 5   LLLFLFLFASSAL-------------SQDSLIGVNIGTEVTNMPSPTQVVALLKSQNINR 64

Query: 71  IRLYDTNPDILKALSGSKIRTLIS----------------------------------GI 130
           +RLYD +  +L A + + ++ +IS                                   I
Sbjct: 65  VRLYDADRSMLLAFAHTGVQVIISVPNDQLLGISQSNATAANWVTRNVAAYYPATNITTI 124

Query: 131 SVGDEVLNTIPSASPLLLPAIESLYNALVAANLQTQITVSTPHAASIILDPFPPSQAFFN 190
           +VG EVL ++ +A+ +L+ A++ +  ALV ANL  QI VSTPH+++IILD FPPSQAFFN
Sbjct: 125 AVGSEVLTSLTNAASVLVSALKYIQAALVTANLDRQIKVSTPHSSTIILDSFPPSQAFFN 184

Query: 191 QSLVQFVLPLLQFLSKTGSPLMMNFYPYYVFMQNKGVVPLDNALFKPLTPSKE------- 250
           ++    ++PLL+FL  TGSPL++N YPY+ ++Q+ GV+PLD ALF+PL  +KE       
Sbjct: 185 KTWDPVIVPLLKFLQSTGSPLLLNVYPYFDYVQSNGVIPLDYALFQPLQANKEAVDANTL 244

Query: 251 ------------------MNLNITDVVVLVTESGWPSKGDSKEPYASIDNADTFNSNLIK 310
                               LN T++ ++VTESGWPSKG   E  A+++NA+T+NSNLI+
Sbjct: 245 LHYTNVFDAIVDAAYFAMSYLNFTNIPIVVTESGWPSKGGPSEHDATVENANTYNSNLIQ 304

Query: 311 HILDRSGTPFHPEVTSSVYIYELFNEDLRSPPVSEANWGLFYGNSTPVNLLHVSGSGTWA 370
           H+++++GTP HP    + YIYEL+NED R                               
Sbjct: 305 HVINKTGTPKHPGTAVTTYIYELYNEDTR------------------------------- 364

Query: 371 CGPGKVLRQLNAPVSEANWGLFYGNSTPVYLLHVSGSGTFLANDTTNQTYCIAMDEFDAK 430
             PG        PVSE NWGLFY N TPVY L ++G+G  LANDTTNQT+CIA ++ D K
Sbjct: 365 --PG--------PVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQTFCIAKEKVDRK 424

Query: 431 TLQTALDWACGPGKANCTEIQPGEACYQPNNVKNHASYAFDSYYQKEGKTAGSCDFKGVA 468
            LQ ALDWACGPGK +C+ +  GE+CY+P++V  H++YAF++YYQK GK +GSCDFKGVA
Sbjct: 425 MLQAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVA 466

BLAST of HG10021059 vs. TAIR 10
Match: AT3G13560.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 317.0 bits (811), Expect = 2.7e-86
Identity = 187/491 (38.09%), Postives = 257/491 (52.34%), Query Frame = 0

Query: 41  FVGV-IWEDVSNLLSPSDLVSFLQFQKITHIRLYDTNPDILKALSGSKIR---------- 100
           F+GV I  D++N+  PSD+V+ L+ Q+ITH+RLYD N  +LKA + + I           
Sbjct: 25  FIGVNIGTDLTNMPPPSDIVTLLKSQQITHVRLYDANSHMLKAFANTSIEVMVGVTNEEI 84

Query: 101 ------------------------TLISGISVGDEVLNTIPSASPLLLPAIESLYNALVA 160
                                   T I+ I+VG EVL TIP  +P+L  A+ +++ ALVA
Sbjct: 85  LKIGRFPSAAAAWVNKNVAAYIPSTNITAIAVGSEVLTTIPHVAPILASALNNIHKALVA 144

Query: 161 ANLQTQITVSTPHAASIILDPFPPSQAFFNQSLVQFVLPLLQFLSKTGSPLMMNFYPYYV 220
           +NL  ++ VS+P +  I+  PFPPS + F+ S    V  LLQFL  TGS  M+N YPYY 
Sbjct: 145 SNLNFKVKVSSPMSMDIMPKPFPPSTSTFSPSWNTTVYQLLQFLKNTGSFFMLNAYPYYG 204

Query: 221 FMQNKGVVPLDNALFKPLTPSKEM-------------------------NLNITDVVVLV 280
           +    G+ PLD ALFK L+P K++                          LN + + V+V
Sbjct: 205 YTTANGIFPLDYALFKQLSPVKQIVDPNTLLHYNSMFDAMVDAAYYSMEALNFSKIPVVV 264

Query: 281 TESGWPSKGDSKEPYASIDNADTFNSNLIKHILDRSGTPFHPEVTSSVYIYELFNEDLRS 340
           TE+GWPS G S E  A++ NA+TFN+NLIK +L+ SG P  P++  + YIYEL+NED RS
Sbjct: 265 TETGWPSSGGSDEAAATVANAETFNTNLIKRVLNNSGPPSQPDIPINTYIYELYNEDKRS 324

Query: 341 PPVSEANWGLFYGNSTPVNLLHVSGSGTWACGPGKVLRQLNAPVSEANWGLFYGNSTPVY 400
            PVSE NWG+ + N T V  L +SG  + A                              
Sbjct: 325 GPVSERNWGILFPNGTSVYPLSLSGGSSSAA----------------------------- 384

Query: 401 LLHVSGSGTFLANDTTNQTYCIAMDEFDAKTLQTALDWACGPGKANCTEIQPGEACYQPN 460
              ++GS  F          C+A  + D   L   L+WACG G+ANC  IQPG+ CY PN
Sbjct: 385 ---LNGSSMF----------CVAKADADDDKLVDGLNWACGQGRANCAAIQPGQPCYLPN 444

Query: 461 NVKNHASYAFDSYYQKEGKTAGSCDFKGVAMITTTDPSHGSCIFPGSKKVRNNTKETVNS 472
           +VK+HAS+AF+ YYQK     G+CDF G A+ TT DPS+ +C + GS           N 
Sbjct: 445 DVKSHASFAFNDYYQKMKSAGGTCDFDGTAITTTRDPSYRTCAYTGSLNA-----NATNG 468