HG10020957 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020957
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCitrulline--aspartate ligase
LocationChr05: 3983212 .. 3986776 (-)
RNA-Seq ExpressionHG10020957
SyntenyHG10020957
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCAGTTAATGGGAGGCTCTCTGTTCTCATCTGCTCCCGTTGGGTTTCACCCATCCAAAACAGGTATCTCGTTTGAAATCTTATTATTATTTTTTCTATTATTCAAAGAGTAAGATTGGAAATGGAAGTGGAAGTGGAAGTGGAAATGGGAATGGGAATGGGAATGTAACACTTTTCATTTTGTTACTTCGAGTTTCTGGCTATTTGTGATTTTTTTTTCTTTCGGGAAGATGATTGGATTAGTGTAATGTCGACGGTAGAGAATATGGTTCTATTGAGTTTTGTGGTTTTTAAAATGCATTATCTGAGGTTGCAGTTTTGTTTTTATTTTCAGAGGCGGTGACGAGTTATTGCAGAATTTCTGGCCTGACGAAGTTGCATTCATTCCAAGAGGCATTTGCATCAATTATCCTCTCCCTCCTATTCTTAAACGATTTTTATCCATATCGATCTGTTCTTATCTTATTCTTGTACTTATTCAATTGCATTCGTGGCTGCAGCTGGGGGCAAAAACGTGTGAAATCCATGGCAATGGCATTGCTAGTGACAGGGCTGGTGTTTTGCGGTCGAATAAGAAAAGCGGTGAGGTTCCCATTGTTGCTTGGTCTGCACTTAATTACCTCCCCCTCCCAGTGTAGTTTTGGAGAAGCAGAGGGAGTTTTATCAGATCGAGCTTTTAATCATGGAGTCATTCTATTAGAAGATTTATATAGATAGTATAGTTGTGTTTATATTTACTCTAAAAGAGCAGCCAAGTGGTATAAAGTGTATTATGAATACTAATTTTGTTCTACTGTTTTAGCTCTGTGAACGAGATGCTTTATAAGATGTGTTTTTGGGGTTTTCCCTTCATATCGAGTTGATAAAAATTTTATCCTTTCTTTTCGAATGGATTCAGTTATTCAGGCAATATTGCAGAGTGAGAGAGAGGTAGAAACTACCAATGGTATAGGGACTAGAGGTTTGCGTGGGCAGTTGAACAAGGTTGTTCTTGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGTAGGTCCCCTGGCTGATGTTTAACCTTGTTTATTGATCTAGCCCTGGCCTTCTAAATTATGAAATACTCATTCAGTTAGTTGAAGTTGCATGATTTGTGAACATTGAAGAATTCAGTGATAGCTGATGCTGGCTCTTCAGTACATCACCTTTTTATGATTATAACCCTTCACTTTGCTTGTACTGCGTGGTTTTTGCTTTGATCTTTCAGTTGTTCTTTCTATGTCATCTCCTTCTGCATTCCTCTTTGGGGATTTGTGCAGCCCTTTTTAGTATCTCCTTTAACTGCTTCTGAATTATTTTCTTCCTATGGGCCTTAACTATTTCATGTATTGCAGGGAGAATTATGGTTGCGAAGTTGTATGTTTTACGGCTGATGTTGGTCAAGTATGTAATTAGCATTGGTAAATTAGTAGTTTTTTTTCTTCCTCTTTTTAAATATATTTATATATATTGGATAGCCACTTGACGGGAAATCCTGTGAAATTTATCTTTTTGCATCTCTTTGTGGTCTTAAACCTGTAGGGCGTGAAGGAATTGGAAGGTTTGGAAAAGAAGGCCAGAGCAAGTGGGGCTTGTCAATTGGTGGTGAAGGATTTAAAGGAAGAATTTGTAAGCGACTTCATATTTCCTTGCTTACGTGCTGGTGCAATTTATGAAAGAAAATACCTGCTTGGGACATCAATGGCCCGTCCAGTTATAGCAAAGGTAAGAACTGACTTCTAGTATTTCCGCGGGTAGTGTCCTTACTTCTAAGTTATATTAACAATGTGGATTTCCCAACAAGAAATAAATTGAAGGTTCTAACTGACTTATAAGCTCCAATCAGATTTATCTTGTTCATGGTTTTTCCTTGTTAGGTTTGGTTTTCTTTTTTGTTTAGTTTGGTATTTTGTAATTTCTTTGTGTTTCTTTTTATATTGAAAATATAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCTGTTTCTCATGGATGCACTGGGAAGGGAAATGATCAGGTATGTAGGCCCTTACACCGTGATTTGGAAATGAATATACTCTTTTCCCTGATTAATTGGTATGGTATGGTAAATCTATTAAGGTACGCTTTGAGTTGGCCTTCTTTGCTCTAAATCCCAATTTAAATGTGGTGGCACCGTGGAGGGAATGGGATATTACTGGAAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATATACCTGTTCCAGTGACAAAGAAATCAATTTACAGTCGAGACAGGAACTTGTGGCATCTTAGTCATGAGGTAATGGTGATTAGTTTTGATTGATTCATCATTTGTCTAAGTTGGATTGTTTATGTTTTTGTTTCTGTTTTGAAGAGTATTGTGCAAACCACCTTTCACAAATGATGTCCGTAGTTTGTCTATCAAGTTTAAATCAACCTAAGTGACGATTTTATTCTAGGATGTATCAAGAAACTGGGTGGATTTTGGCCTCTTTATGAATTTTATCCCTTTCTGATGTTCTCTTGTCCATTATTACTGAAGGGAGACGTTTTGGAGGATCCAGCAAACGAACCAAAGAAGGACATGTACATGATGTCTACGGATCCAGAAGATGCACCAAACGAACCAGAGTAAACTTCCTTTTCTTTAACTCCAAATCTTTGCAATAATGATTCTATTATTGAATCTGTCTTCAACCATTCAAACTTCTCTCAACAACAGTTGGTTGTGTTGAAGGACTAATAGGTCAGTCTAGAATCAAGTTAATTCTATTTGCTACACACAGCTGGAAAATAAAGAGTTCTGTTTAAATAGTATGAAAAAGAGAGGCACTTTTAGTAATTCAATATTCGAGTTTGAGATTCATAATATAAAGAGAATGCTTTCATAACTCATTTTCTTTGCTTAATTTGTGGGCCATACCTTAAGTCTCTTTGGTGGGTGTTTGCCATTTCTGTTCAACGAGCTTAACATGCCATGATCTTGTGCAGATATGTGGAAATAGGAATTGTGGGAGGTCTCCCAGTTTCTGTCAATGGCGAGGTTCTTTCACCAGCCTCTCTTCTGGCCAAAGTAAACGAAATTGGTGGAAAGCATGGGATTGGGAGAATTGACATGGTTGAAAACCGTCTGGTGGGTATGAAAAGTAGAGGTGTCTATGAAACCCCAGGAGGAACCATCCTTTTCGCAGCCACCCGTGAGCTCGAGTCTCTAACACTTGACCGAGAGACAATTCAAGTGAAAGACTCATTGGCCCTCAAATATGCAGAGTTGGTTTATGCAGGAAGATGGTTCGACCCTCTTCGTGAATCCATGGATGCTTTCATGGAGAAGATCACAGAGACCACCACTGGCTCCGTCACTCTCAAGCTCTACAAGGGTTCCGTCCATGTGGCCAGTCGCCGGAGTGTATACAGTCTTTACAGGGAAGATATCTCCTCCTTTGAAAATGGGCAGATTTACAATCAGGCTGATGCTGCTGGTTTTATCCGCCTTTATGGCCTTCCCACCAGAGTCCGGGCAATGCACCATAAAGACTTTTGA

mRNA sequence

ATGGCTCAGTTAATGGGAGGCTCTCTGTTCTCATCTGCTCCCGTTGGGTTTCACCCATCCAAAACAGTTATTCAGGCAATATTGCAGAGTGAGAGAGAGGTAGAAACTACCAATGGTATAGGGACTAGAGGTTTGCGTGGGCAGTTGAACAAGGTTGTTCTTGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGGAGAATTATGGTTGCGAAGTTGTATGTTTTACGGCTGATGTTGGTCAAGGCGTGAAGGAATTGGAAGGTTTGGAAAAGAAGGCCAGAGCAAGTGGGGCTTGTCAATTGGTGGTGAAGGATTTAAAGGAAGAATTTGTAAGCGACTTCATATTTCCTTGCTTACGTGCTGGTGCAATTTATGAAAGAAAATACCTGCTTGGGACATCAATGGCCCGTCCAGTTATAGCAAAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCTGTTTCTCATGGATGCACTGGGAAGGGAAATGATCAGGTACGCTTTGAGTTGGCCTTCTTTGCTCTAAATCCCAATTTAAATGTGGTGGCACCGTGGAGGGAATGGGATATTACTGGAAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATATACCTGTTCCAGTGACAAAGAAATCAATTTACAGTCGAGACAGGAACTTGTGGCATCTTAGTCATGAGGGAGACGTTTTGGAGGATCCAGCAAACGAACCAAAGAAGGACATGTACATGATGTCTACGGATCCAGAAGATGCACCAAACGAACCAGAATATGTGGAAATAGGAATTGTGGGAGGTCTCCCAGTTTCTGTCAATGGCGAGGTTCTTTCACCAGCCTCTCTTCTGGCCAAAGTAAACGAAATTGGTGGAAAGCATGGGATTGGGAGAATTGACATGGTTGAAAACCGTCTGGTGGGTATGAAAAGTAGAGGTGTCTATGAAACCCCAGGAGGAACCATCCTTTTCGCAGCCACCCGTGAGCTCGAGTCTCTAACACTTGACCGAGAGACAATTCAAGTGAAAGACTCATTGGCCCTCAAATATGCAGAGTTGGTTTATGCAGGAAGATGGTTCGACCCTCTTCGTGAATCCATGGATGCTTTCATGGAGAAGATCACAGAGACCACCACTGGCTCCGTCACTCTCAAGCTCTACAAGGGTTCCGTCCATGTGGCCAGTCGCCGGAGTGTATACAGTCTTTACAGGGAAGATATCTCCTCCTTTGAAAATGGGCAGATTTACAATCAGGCTGATGCTGCTGGTTTTATCCGCCTTTATGGCCTTCCCACCAGAGTCCGGGCAATGCACCATAAAGACTTTTGA

Coding sequence (CDS)

ATGGCTCAGTTAATGGGAGGCTCTCTGTTCTCATCTGCTCCCGTTGGGTTTCACCCATCCAAAACAGTTATTCAGGCAATATTGCAGAGTGAGAGAGAGGTAGAAACTACCAATGGTATAGGGACTAGAGGTTTGCGTGGGCAGTTGAACAAGGTTGTTCTTGCTTACAGTGGTGGCTTAGACACATCAGTCATCGTCCCCTGGCTGAGGGAGAATTATGGTTGCGAAGTTGTATGTTTTACGGCTGATGTTGGTCAAGGCGTGAAGGAATTGGAAGGTTTGGAAAAGAAGGCCAGAGCAAGTGGGGCTTGTCAATTGGTGGTGAAGGATTTAAAGGAAGAATTTGTAAGCGACTTCATATTTCCTTGCTTACGTGCTGGTGCAATTTATGAAAGAAAATACCTGCTTGGGACATCAATGGCCCGTCCAGTTATAGCAAAGGCCATGGTGGATGTTGCCAAAGAAGTTGGAGCTGATGCTGTTTCTCATGGATGCACTGGGAAGGGAAATGATCAGGTACGCTTTGAGTTGGCCTTCTTTGCTCTAAATCCCAATTTAAATGTGGTGGCACCGTGGAGGGAATGGGATATTACTGGAAGAGAAGATGCTATTGAGTATGCCAAGAAGCATAATATACCTGTTCCAGTGACAAAGAAATCAATTTACAGTCGAGACAGGAACTTGTGGCATCTTAGTCATGAGGGAGACGTTTTGGAGGATCCAGCAAACGAACCAAAGAAGGACATGTACATGATGTCTACGGATCCAGAAGATGCACCAAACGAACCAGAATATGTGGAAATAGGAATTGTGGGAGGTCTCCCAGTTTCTGTCAATGGCGAGGTTCTTTCACCAGCCTCTCTTCTGGCCAAAGTAAACGAAATTGGTGGAAAGCATGGGATTGGGAGAATTGACATGGTTGAAAACCGTCTGGTGGGTATGAAAAGTAGAGGTGTCTATGAAACCCCAGGAGGAACCATCCTTTTCGCAGCCACCCGTGAGCTCGAGTCTCTAACACTTGACCGAGAGACAATTCAAGTGAAAGACTCATTGGCCCTCAAATATGCAGAGTTGGTTTATGCAGGAAGATGGTTCGACCCTCTTCGTGAATCCATGGATGCTTTCATGGAGAAGATCACAGAGACCACCACTGGCTCCGTCACTCTCAAGCTCTACAAGGGTTCCGTCCATGTGGCCAGTCGCCGGAGTGTATACAGTCTTTACAGGGAAGATATCTCCTCCTTTGAAAATGGGCAGATTTACAATCAGGCTGATGCTGCTGGTTTTATCCGCCTTTATGGCCTTCCCACCAGAGTCCGGGCAATGCACCATAAAGACTTTTGA

Protein sequence

MAQLMGGSLFSSAPVGFHPSKTVIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLYGLPTRVRAMHHKDF
Homology
BLAST of HG10020957 vs. NCBI nr
Match: XP_008439546.1 (PREDICTED: argininosuccinate synthase, chloroplastic isoform X1 [Cucumis melo] >KAA0055325.1 argininosuccinate synthase [Cucumis melo var. makuwa] >TYJ99250.1 argininosuccinate synthase [Cucumis melo var. makuwa])

HSP 1 Score: 864.0 bits (2231), Expect = 5.7e-247
Identity = 441/494 (89.27%), Postives = 443/494 (89.68%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPSKT-------------------------------------- 60
           MAQLMGGSLFSSAPVGFHPSKT                                      
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVAGYCRISCLTKLHSFQELGAKTCEIHGNGLSSNSS 60

Query: 61  ---------VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                    VIQAILQSERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  GVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK
Sbjct: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
            DPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 ADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 448
           AFMEKITETTTGSVTLKLYKGSVHVASRRS YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. NCBI nr
Match: XP_004152522.1 (argininosuccinate synthase, chloroplastic [Cucumis sativus] >KGN64401.1 hypothetical protein Csa_013317 [Cucumis sativus])

HSP 1 Score: 859.8 bits (2220), Expect = 1.1e-245
Identity = 437/494 (88.46%), Postives = 442/494 (89.47%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPSKT-------------------------------------- 60
           MAQLMGGSLFSSAPVGFHPSKT                                      
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVASYCRISSLTKLHSFQELGAKTCEIHGNGLSSNSA 60

Query: 61  ---------VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                    VIQAILQSERE+ETTNGIGT GLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  GVLRSNKKRVIQAILQSEREIETTNGIGTGGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQG+KELEGLE KARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK
Sbjct: 121 GCEVVCFTADVGQGMKELEGLENKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
            DPEDAPN+PEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 ADPEDAPNKPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAA RELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAAARELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 448
           AFMEKITETTTGSVTLKLYKGSVHVASRRS+YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. NCBI nr
Match: XP_038895641.1 (argininosuccinate synthase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 859.4 bits (2219), Expect = 1.4e-245
Identity = 438/493 (88.84%), Postives = 440/493 (89.25%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPSKT-------------------------------------- 60
           MAQLMGGSLFS APVGFHPSKT                                      
Sbjct: 1   MAQLMGGSLFSYAPVGFHPSKTEAVASYCRVSGLTKLHSFQELGAKTCEIHGNSIASNRA 60

Query: 61  ---------VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                    VIQAILQSERE+ETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  GVLQLNKKSVIQAILQSEREIETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQG KELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK
Sbjct: 121 GCEVVCFTADVGQGAKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
           TDPEDAPNEPEYVEIGIV GLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 TDPEDAPNEPEYVEIGIVRGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 447
           AFMEKITETTTGS TLKLYKGSVHVASRRS+YSLYREDISSFENGQIYNQADA GFIRLY
Sbjct: 421 AFMEKITETTTGSATLKLYKGSVHVASRRSLYSLYREDISSFENGQIYNQADADGFIRLY 480

BLAST of HG10020957 vs. NCBI nr
Match: KAG7019736.1 (Argininosuccinate synthase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 849.7 bits (2194), Expect = 1.1e-242
Identity = 429/491 (87.37%), Postives = 438/491 (89.21%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPS---------------------------------------- 60
           MAQLMGGSLFS+AP GFHPS                                        
Sbjct: 1   MAQLMGGSLFSTAPGGFHPSELVASYCRISGPTKLHSFQELGAKASEIHGNSIACNRASV 60

Query: 61  -----KTVIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENYGC 120
                K+VIQAILQSERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENYGC
Sbjct: 61  LRSNKKSVIQAILQSEREIETTNGITTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENYGC 120

Query: 121 EVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERKYL 180
           EVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKEEFV D+IFPCLRAGA+YERKYL
Sbjct: 121 EVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKEEFVRDYIFPCLRAGAVYERKYL 180

Query: 181 LGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWREW 240
           LGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWREW
Sbjct: 181 LGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWREW 240

Query: 241 DITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMSTD 300
           DITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSHEGD+LEDPANEPKKDMYM+STD
Sbjct: 241 DITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDILEDPANEPKKDMYMISTD 300

Query: 301 PEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVGMK 360
           PEDAPNEPEYVEIGIVGGLPVS+NGEVLSPASLL KVNEIGGKHGIGRIDMVENRLVGMK
Sbjct: 301 PEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVNEIGGKHGIGRIDMVENRLVGMK 360

Query: 361 SRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMDAF 420
           SRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMDAF
Sbjct: 361 SRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMDAF 420

Query: 421 MEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLYGL 447
           MEKITETTTGSVTLKLYKGSVHV SRRS YSLYREDISSFENGQIYNQADAAGFIRLYGL
Sbjct: 421 MEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYREDISSFENGQIYNQADAAGFIRLYGL 480

BLAST of HG10020957 vs. NCBI nr
Match: XP_023519539.1 (argininosuccinate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 848.2 bits (2190), Expect = 3.2e-242
Identity = 429/493 (87.02%), Postives = 438/493 (88.84%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPS---------------------------------------- 60
           MAQLMGGSLFS+AP GFHPS                                        
Sbjct: 1   MAQLMGGSLFSTAPGGFHPSKAESVASYCRISGPTKLHSFQELGAKASEIHGNSIACNRA 60

Query: 61  -------KTVIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                  K+VIQAILQSERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  SVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKEEFV D+IFPCLRAGA+YERK
Sbjct: 121 GCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKEEFVRDYIFPCLRAGAVYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYM+S
Sbjct: 241 EWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMIS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
           TDPEDAPNEPEYVEIGIVGGLPVS+NGEVLSPASLL KVNE+GGKHGIGRIDMVENRLVG
Sbjct: 301 TDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVNEMGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 447
           AFMEKITETTTGSVTLKLYKGSVHV SRRS YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. ExPASy Swiss-Prot
Match: Q9SZX3 (Argininosuccinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g24830 PE=1 SV=3)

HSP 1 Score: 727.2 bits (1876), Expect = 1.1e-208
Identity = 359/445 (80.67%), Postives = 396/445 (88.99%), Query Frame = 0

Query: 2   AQLMGGSLFSSAPVGFHPSKT-VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGL 61
           +QL+G ++ +    G    K   I+A+L  +    TT+     GLRG+L KVVLAYSGGL
Sbjct: 49  SQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKVVLAYSGGL 108

Query: 62  DTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFI 121
           DTSVIVPWL+ENYGCEVVCFTADVGQG+KELEGLE+KA+ASGA QLVVKDL EEFV DFI
Sbjct: 109 DTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLTEEFVKDFI 168

Query: 122 FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFF 181
           FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQVRFEL FF
Sbjct: 169 FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQVRFELTFF 228

Query: 182 ALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLED 241
           +LNP L VVAPWREW+I GREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSHEGD+LED
Sbjct: 229 SLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDLLED 288

Query: 242 PANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHG 301
           PANEPKKDMYMMS DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LLA++N IGGKHG
Sbjct: 289 PANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAELNTIGGKHG 348

Query: 302 IGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVY 361
           IGRIDMVENRLVGMKSRGVYETPGGTILFAA +ELESLTLDRE+IQVKD+LALKYAE+VY
Sbjct: 349 IGRIDMVENRLVGMKSRGVYETPGGTILFAAVQELESLTLDRESIQVKDTLALKYAEMVY 408

Query: 362 AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQI 421
           AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V  R+S  SLYR+DISSFE  +I
Sbjct: 409 AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDISSFEGSEI 468

Query: 422 YNQADAAGFIRLYGLPTRVRAMHHK 446
           YNQADAAGFIRLYGLP ++RAM  K
Sbjct: 469 YNQADAAGFIRLYGLPMKIRAMLKK 492

BLAST of HG10020957 vs. ExPASy Swiss-Prot
Match: Q3A9W5 (Argininosuccinate synthase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901) OX=246194 GN=argG PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 3.2e-152
Identity = 258/397 (64.99%), Postives = 319/397 (80.35%), Query Frame = 0

Query: 51  KVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKD 110
           KVVLAYSGGLDTS+I+PWL+ENYG EV+   ADVGQG +ELE L +KA  SGA ++ ++D
Sbjct: 4   KVVLAYSGGLDTSIIIPWLKENYGYEVIAMVADVGQG-EELEPLREKAIKSGAAKIYIED 63

Query: 111 LKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGN 170
           +KEEFV DF+FP L+AGA+YE KYLLGTS+ARP+IAK +V++A++ GA AV+HG TGKGN
Sbjct: 64  VKEEFVRDFVFPMLKAGAVYENKYLLGTSVARPLIAKKLVEIAEKEGAVAVAHGATGKGN 123

Query: 171 DQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWH 230
           DQVRFEL   ALNP+L ++APWREW+I  REDAI+YA+K  IPVPVTKK  YS DRNLWH
Sbjct: 124 DQVRFELTVKALNPDLKIIAPWREWEIKSREDAIDYAEKRGIPVPVTKKQPYSMDRNLWH 183

Query: 231 LSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLA 290
           LSHEG +LEDPA EP +D+ +++  PE AP++PEYVEI  V G PV+VNGE LSP  L+ 
Sbjct: 184 LSHEGGILEDPAVEPPEDVLLLTNPPEKAPDQPEYVEIEFVKGEPVAVNGEKLSPVELIF 243

Query: 291 KVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDS 350
           K+NE+GGKHGIG  DMVENRLVGMKSRGVYETPGGTIL  A RELESLTLDR+T+  K  
Sbjct: 244 KLNELGGKHGIGIADMVENRLVGMKSRGVYETPGGTILTFAHRELESLTLDRQTMHFKQM 303

Query: 351 LALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYRE 410
           +ALKYAEL+Y G WF PLRE+++AF++K  ET TG V +KLYKG+++ A   S YSLY +
Sbjct: 304 VALKYAELIYDGLWFTPLREALEAFVDKTQETVTGKVRVKLYKGNIYPAGITSPYSLYVK 363

Query: 411 DISSFENGQIYNQADAAGFIRLYGLPTRVRAMHHKDF 448
           D+++F    +YNQ DA GFI L+GLP +VRAM  K +
Sbjct: 364 DLATFGEDNLYNQKDAEGFINLFGLPLKVRAMTQKPY 399

BLAST of HG10020957 vs. ExPASy Swiss-Prot
Match: B8FWX2 (Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain DSM 10664 / DCB-2) OX=272564 GN=argG PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 9.1e-147
Identity = 249/397 (62.72%), Postives = 311/397 (78.34%), Query Frame = 0

Query: 49  LNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVV 108
           + KVVLAYSGGLDTS+I+PWL+ENYG EV+   AD+GQG +ELE L +KA  SGA +L +
Sbjct: 1   MKKVVLAYSGGLDTSIIIPWLKENYGYEVIAMAADLGQG-EELEPLHEKAIKSGASKLYI 60

Query: 109 KDLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGK 168
           +DL+EEFV+DFI+P L+AGA+YE KYLLGTS ARP+IA+ +V++A + GA A++HG TGK
Sbjct: 61  EDLQEEFVTDFIYPTLKAGAVYEGKYLLGTSFARPLIAQRLVEIAAKEGAVAIAHGATGK 120

Query: 169 GNDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNL 228
           GNDQVRFELA  ALNP+L ++APWR WDI  REDAI+YA +  IPVPVTK   YS DRN+
Sbjct: 121 GNDQVRFELAVKALNPDLEIIAPWRIWDIKSREDAIDYAVERGIPVPVTKDRPYSMDRNV 180

Query: 229 WHLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASL 288
           WHLSHEG  LEDP NEPKKD+Y++   PEDAP+E EY+E+    G+PVS+NGE L P  L
Sbjct: 181 WHLSHEGGDLEDPWNEPKKDLYLLGVSPEDAPDEAEYLELDFEQGIPVSLNGEKLGPVQL 240

Query: 289 LAKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVK 348
           L  +NE+GGKHGIG +DMVENRLVGMKSRGVYETPGGTIL+ A + LE LTLDR T+  K
Sbjct: 241 LETLNEVGGKHGIGIVDMVENRLVGMKSRGVYETPGGTILYTAHQALEHLTLDRLTLHYK 300

Query: 349 DSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLY 408
           + +ALKYAELVY G W  PLRE++DAF++   +  TG+V LKLYKG+  +A  +S YSLY
Sbjct: 301 EQIALKYAELVYDGVWHSPLREALDAFVDVTQKNVTGTVRLKLYKGNCSLAGVKSPYSLY 360

Query: 409 REDISSFENGQIYNQADAAGFIRLYGLPTRVRAMHHK 446
            E+ ++F    +YNQ DA GFI L+GLP +VRA+  K
Sbjct: 361 SEEFATFGRDGVYNQKDAEGFINLFGLPLKVRALMEK 396

BLAST of HG10020957 vs. ExPASy Swiss-Prot
Match: Q24ZG8 (Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain Y51) OX=138119 GN=argG PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 9.1e-147
Identity = 249/397 (62.72%), Postives = 311/397 (78.34%), Query Frame = 0

Query: 49  LNKVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVV 108
           + KVVLAYSGGLDTS+I+PWL+ENYG EV+   AD+GQG +ELE L +KA  SGA +L +
Sbjct: 9   MKKVVLAYSGGLDTSIIIPWLKENYGYEVIAMAADLGQG-EELEPLHEKAIKSGASKLYI 68

Query: 109 KDLKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGK 168
           +DL+EEFV+DFI+P L+AGA+YE KYLLGTS ARP+IA+ +V++A + GA A++HG TGK
Sbjct: 69  EDLQEEFVTDFIYPTLKAGAVYEGKYLLGTSFARPLIAQRLVEIAAKEGAVAIAHGATGK 128

Query: 169 GNDQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNL 228
           GNDQVRFELA  ALNP+L ++APWR WDI  REDAI+YA +  IPVPVTK   YS DRN+
Sbjct: 129 GNDQVRFELAVKALNPDLEIIAPWRIWDIKSREDAIDYAVERGIPVPVTKDRPYSMDRNV 188

Query: 229 WHLSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASL 288
           WHLSHEG  LEDP NEPKKD+Y++   PEDAP+E EY+E+    G+PVS+NGE L P  L
Sbjct: 189 WHLSHEGGDLEDPWNEPKKDLYLLGVSPEDAPDEAEYLELDFEQGIPVSLNGEKLGPVQL 248

Query: 289 LAKVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVK 348
           L  +NE+GGKHGIG +DMVENRLVGMKSRGVYETPGGTIL+ A + LE LTLDR T+  K
Sbjct: 249 LETLNEVGGKHGIGIVDMVENRLVGMKSRGVYETPGGTILYTAHQALEHLTLDRLTLHYK 308

Query: 349 DSLALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLY 408
           + +ALKYAELVY G W  PLRE++DAF++   +  TG+V LKLYKG+  +A  +S YSLY
Sbjct: 309 EQIALKYAELVYDGVWHSPLREALDAFVDVTQKNVTGTVRLKLYKGNCSLAGVKSPYSLY 368

Query: 409 REDISSFENGQIYNQADAAGFIRLYGLPTRVRAMHHK 446
            E+ ++F    +YNQ DA GFI L+GLP +VRA+  K
Sbjct: 369 SEEFATFGRDGVYNQKDAEGFINLFGLPLKVRALMEK 404

BLAST of HG10020957 vs. ExPASy Swiss-Prot
Match: A4J173 (Argininosuccinate synthase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=argG PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.0e-146
Identity = 254/395 (64.30%), Postives = 312/395 (78.99%), Query Frame = 0

Query: 51  KVVLAYSGGLDTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKD 110
           KVVLAYSGGLDTSVI+ WL+ENYG EV+  TAD+GQG +EL  LE+KA  SGA ++ ++D
Sbjct: 3   KVVLAYSGGLDTSVIIAWLKENYGYEVIAVTADLGQG-EELAPLEEKALQSGASKIYIED 62

Query: 111 LKEEFVSDFIFPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGN 170
           L++EFV D+I+P L+AGAIYE KYLLGTS ARP+IAK +V++A++ GA AV+HG TGKGN
Sbjct: 63  LRKEFVEDYIWPTLKAGAIYEGKYLLGTSFARPLIAKKLVEIAEKEGAVAVAHGATGKGN 122

Query: 171 DQVRFELAFFALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWH 230
           DQVRFEL   AL P+L V+APWREWDI  REDAI+YA+   IPVPVTKKSIYSRDRN+WH
Sbjct: 123 DQVRFELGVKALAPHLKVIAPWREWDIRSREDAIDYAEARGIPVPVTKKSIYSRDRNIWH 182

Query: 231 LSHEGDVLEDPANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLA 290
           +SHEG  LE PAN    DM M++  PE AP++P YVEIG   G+PVS+NGE++    LL 
Sbjct: 183 ISHEGGELESPANAASYDMLMLTVPPEKAPDQPTYVEIGFEKGIPVSINGELMDSIGLLE 242

Query: 291 KVNEIGGKHGIGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDS 350
           K+N IGG++GIG +DMVENRLVGMKSRGVYETPGG IL  A RELE LTLDR T+  K+ 
Sbjct: 243 KLNVIGGENGIGIVDMVENRLVGMKSRGVYETPGGAILVYAHRELELLTLDRATLHYKEQ 302

Query: 351 LALKYAELVYAGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYRE 410
           +AL+YAELVY G WF PLRE++DAF++    T TG+V LKLYKG++  A   S YSLY E
Sbjct: 303 IALRYAELVYDGVWFAPLREALDAFVDNTQRTVTGTVKLKLYKGNMMPAGVTSPYSLYDE 362

Query: 411 DISSFENGQIYNQADAAGFIRLYGLPTRVRAMHHK 446
           ++S+F   ++YNQADAAGFI L+GLP +VRAM  K
Sbjct: 363 ELSTFGRDEVYNQADAAGFINLFGLPLKVRAMMEK 396

BLAST of HG10020957 vs. ExPASy TrEMBL
Match: A0A5D3BHJ5 (Citrulline--aspartate ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004610 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.8e-247
Identity = 441/494 (89.27%), Postives = 443/494 (89.68%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPSKT-------------------------------------- 60
           MAQLMGGSLFSSAPVGFHPSKT                                      
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVAGYCRISCLTKLHSFQELGAKTCEIHGNGLSSNSS 60

Query: 61  ---------VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                    VIQAILQSERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  GVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK
Sbjct: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
            DPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 ADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 448
           AFMEKITETTTGSVTLKLYKGSVHVASRRS YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. ExPASy TrEMBL
Match: A0A1S3AZP1 (Citrulline--aspartate ligase OS=Cucumis melo OX=3656 GN=LOC103484309 PE=3 SV=1)

HSP 1 Score: 864.0 bits (2231), Expect = 2.8e-247
Identity = 441/494 (89.27%), Postives = 443/494 (89.68%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPSKT-------------------------------------- 60
           MAQLMGGSLFSSAPVGFHPSKT                                      
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVAGYCRISCLTKLHSFQELGAKTCEIHGNGLSSNSS 60

Query: 61  ---------VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                    VIQAILQSERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  GVRRSNKKRVIQAILQSEREIETTNGIVTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK
Sbjct: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
            DPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 ADPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 448
           AFMEKITETTTGSVTLKLYKGSVHVASRRS YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSHYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. ExPASy TrEMBL
Match: A0A0A0LWM9 (Citrulline--aspartate ligase OS=Cucumis sativus OX=3659 GN=Csa_1G050400 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.2e-246
Identity = 437/494 (88.46%), Postives = 442/494 (89.47%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPSKT-------------------------------------- 60
           MAQLMGGSLFSSAPVGFHPSKT                                      
Sbjct: 1   MAQLMGGSLFSSAPVGFHPSKTEAVASYCRISSLTKLHSFQELGAKTCEIHGNGLSSNSA 60

Query: 61  ---------VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                    VIQAILQSERE+ETTNGIGT GLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  GVLRSNKKRVIQAILQSEREIETTNGIGTGGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQG+KELEGLE KARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK
Sbjct: 121 GCEVVCFTADVGQGMKELEGLENKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
            DPEDAPN+PEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 ADPEDAPNKPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAA RELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAAARELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 448
           AFMEKITETTTGSVTLKLYKGSVHVASRRS+YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSLYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. ExPASy TrEMBL
Match: A0A6J1E7A9 (Citrulline--aspartate ligase OS=Cucurbita moschata OX=3662 GN=LOC111431281 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 3.5e-242
Identity = 429/493 (87.02%), Postives = 437/493 (88.64%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPS---------------------------------------- 60
           MAQLMGG LFS+AP GFHPS                                        
Sbjct: 1   MAQLMGGFLFSTAPGGFHPSKAESVASYCRISGPTKLHSFQELGAKASEIHGNSIACNRA 60

Query: 61  -------KTVIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                  K+VIQAILQSERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  SVLRSNKKSVIQAILQSEREIETTNGITTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKEEFV D+IFPCLRAGA+YERK
Sbjct: 121 GCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKEEFVRDYIFPCLRAGAVYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGD+LEDPANEPKKDMYM+S
Sbjct: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDILEDPANEPKKDMYMIS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
           TDPEDAPNEPEYVEIGIVGGLPVS+NGEVLSPASLL KVNEIGGKHGIGRIDMVENRLVG
Sbjct: 301 TDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVNEIGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 447
           AFMEKITETTTGSVTLKLYKGSVHV SRRS YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVVSRRSQYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. ExPASy TrEMBL
Match: A0A6J1KF74 (Citrulline--aspartate ligase OS=Cucurbita maxima OX=3661 GN=LOC111495262 PE=3 SV=1)

HSP 1 Score: 843.6 bits (2178), Expect = 3.9e-241
Identity = 427/493 (86.61%), Postives = 437/493 (88.64%), Query Frame = 0

Query: 1   MAQLMGGSLFSSAPVGFHPS---------------------------------------- 60
           MAQLMGGSLFS+AP GFHPS                                        
Sbjct: 1   MAQLMGGSLFSTAPGGFHPSKAESVASYCRISGSTKLHSFQELGAKASEIHGNSIACNMA 60

Query: 61  -------KTVIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120
                  K+VIQAIL SERE+ETTNGI TRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY
Sbjct: 61  SVLRSNKKSVIQAILPSERELETTNGITTRGLRGQLNKVVLAYSGGLDTSVIVPWLRENY 120

Query: 121 GCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFIFPCLRAGAIYERK 180
           GCEVVCFTADVGQGV ELEGLEKKARASGACQLVVKDLKEEFV D+IFPCLRAGA+YERK
Sbjct: 121 GCEVVCFTADVGQGVMELEGLEKKARASGACQLVVKDLKEEFVRDYIFPCLRAGAVYERK 180

Query: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240
           YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR
Sbjct: 181 YLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFFALNPNLNVVAPWR 240

Query: 241 EWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMMS 300
           EWDITGREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYM+S
Sbjct: 241 EWDITGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDVLEDPANEPKKDMYMIS 300

Query: 301 TDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHGIGRIDMVENRLVG 360
           TDPEDAPNEPEYVEIGIVGGLPVS+NGEVLSPASLL KVNE+GGKHGIGRIDMVENRLVG
Sbjct: 301 TDPEDAPNEPEYVEIGIVGGLPVSINGEVLSPASLLTKVNEMGGKHGIGRIDMVENRLVG 360

Query: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420
           MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD
Sbjct: 361 MKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVYAGRWFDPLRESMD 420

Query: 421 AFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQIYNQADAAGFIRLY 447
           AFMEKITETTTGSVTLKLYKGSVHV SR+S YSLYREDISSFENGQIYNQADAAGFIRLY
Sbjct: 421 AFMEKITETTTGSVTLKLYKGSVHVVSRQSQYSLYREDISSFENGQIYNQADAAGFIRLY 480

BLAST of HG10020957 vs. TAIR 10
Match: AT4G24830.1 (arginosuccinate synthase family )

HSP 1 Score: 727.2 bits (1876), Expect = 7.8e-210
Identity = 359/445 (80.67%), Postives = 396/445 (88.99%), Query Frame = 0

Query: 2   AQLMGGSLFSSAPVGFHPSKT-VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGL 61
           +QL+G ++ +    G    K   I+A+L  +    TT+     GLRG+L KVVLAYSGGL
Sbjct: 49  SQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKVVLAYSGGL 108

Query: 62  DTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFI 121
           DTSVIVPWL+ENYGCEVVCFTADVGQG+KELEGLE+KA+ASGA QLVVKDL EEFV DFI
Sbjct: 109 DTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLTEEFVKDFI 168

Query: 122 FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFF 181
           FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQVRFEL FF
Sbjct: 169 FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQVRFELTFF 228

Query: 182 ALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLED 241
           +LNP L VVAPWREW+I GREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSHEGD+LED
Sbjct: 229 SLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDLLED 288

Query: 242 PANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHG 301
           PANEPKKDMYMMS DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LLA++N IGGKHG
Sbjct: 289 PANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAELNTIGGKHG 348

Query: 302 IGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVY 361
           IGRIDMVENRLVGMKSRGVYETPGGTILFAA +ELESLTLDRE+IQVKD+LALKYAE+VY
Sbjct: 349 IGRIDMVENRLVGMKSRGVYETPGGTILFAAVQELESLTLDRESIQVKDTLALKYAEMVY 408

Query: 362 AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQI 421
           AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V  R+S  SLYR+DISSFE  +I
Sbjct: 409 AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDISSFEGSEI 468

Query: 422 YNQADAAGFIRLYGLPTRVRAMHHK 446
           YNQADAAGFIRLYGLP ++RAM  K
Sbjct: 469 YNQADAAGFIRLYGLPMKIRAMLKK 492

BLAST of HG10020957 vs. TAIR 10
Match: AT4G24830.2 (arginosuccinate synthase family )

HSP 1 Score: 629.4 bits (1622), Expect = 2.2e-180
Identity = 320/445 (71.91%), Postives = 353/445 (79.33%), Query Frame = 0

Query: 2   AQLMGGSLFSSAPVGFHPSKT-VIQAILQSEREVETTNGIGTRGLRGQLNKVVLAYSGGL 61
           +QL+G ++ +    G    K   I+A+L  +    TT+     GLRG+L KVVLAYSGGL
Sbjct: 49  SQLVGNAVVTGHVTGSRSCKNQAIRAVLSGDGTALTTDS-KEAGLRGKLKKVVLAYSGGL 108

Query: 62  DTSVIVPWLRENYGCEVVCFTADVGQGVKELEGLEKKARASGACQLVVKDLKEEFVSDFI 121
           DTSVIVPWL+ENYGCEVVCFTADVGQG+KELEGLE+KA+ASGA QLVVKDL EEFV DFI
Sbjct: 109 DTSVIVPWLKENYGCEVVCFTADVGQGIKELEGLEQKAKASGASQLVVKDLTEEFVKDFI 168

Query: 122 FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAKEVGADAVSHGCTGKGNDQVRFELAFF 181
           FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVA EVGADAV+HGCTGKGNDQVRFEL FF
Sbjct: 169 FPCLRAGAIYERKYLLGTSMARPVIAKAMVDVAAEVGADAVAHGCTGKGNDQVRFELTFF 228

Query: 182 ALNPNLNVVAPWREWDITGREDAIEYAKKHNIPVPVTKKSIYSRDRNLWHLSHEGDVLED 241
           +LNP L VVAPWREW+I GREDAIEYAKKHN+PVPVTKKSIYSRDRNLWHLSHEGD+LED
Sbjct: 229 SLNPELKVVAPWREWEIQGREDAIEYAKKHNVPVPVTKKSIYSRDRNLWHLSHEGDLLED 288

Query: 242 PANEPKKDMYMMSTDPEDAPNEPEYVEIGIVGGLPVSVNGEVLSPASLLAKVNEIGGKHG 301
           PANEPKKDMYMMS DPEDAP++PEY+EIGI  GLPV++NG+ LSPA+LLA++N IGGKHG
Sbjct: 289 PANEPKKDMYMMSVDPEDAPDQPEYIEIGIESGLPVALNGKALSPATLLAELNTIGGKHG 348

Query: 302 IGRIDMVENRLVGMKSRGVYETPGGTILFAATRELESLTLDRETIQVKDSLALKYAELVY 361
           IGRIDMVENRLVGMKSR                                           
Sbjct: 349 IGRIDMVENRLVGMKSR------------------------------------------- 408

Query: 362 AGRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVHVASRRSVYSLYREDISSFENGQI 421
            GRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSV V  R+S  SLYR+DISSFE  +I
Sbjct: 409 -GRWFDPLRESMDAFMEKITETTTGSVTLKLYKGSVSVTGRQSPNSLYRQDISSFEGSEI 448

Query: 422 YNQADAAGFIRLYGLPTRVRAMHHK 446
           YNQADAAGFIRLYGLP ++RAM  K
Sbjct: 469 YNQADAAGFIRLYGLPMKIRAMLKK 448

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439546.15.7e-24789.27PREDICTED: argininosuccinate synthase, chloroplastic isoform X1 [Cucumis melo] >... [more]
XP_004152522.11.1e-24588.46argininosuccinate synthase, chloroplastic [Cucumis sativus] >KGN64401.1 hypothet... [more]
XP_038895641.11.4e-24588.84argininosuccinate synthase, chloroplastic-like [Benincasa hispida][more]
KAG7019736.11.1e-24287.37Argininosuccinate synthase, chloroplastic [Cucurbita argyrosperma subsp. argyros... [more]
XP_023519539.13.2e-24287.02argininosuccinate synthase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SZX31.1e-20880.67Argininosuccinate synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4... [more]
Q3A9W53.2e-15264.99Argininosuccinate synthase OS=Carboxydothermus hydrogenoformans (strain ATCC BAA... [more]
B8FWX29.1e-14762.72Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain DSM 10664 / D... [more]
Q24ZG89.1e-14762.72Argininosuccinate synthase OS=Desulfitobacterium hafniense (strain Y51) OX=13811... [more]
A4J1732.0e-14664.30Argininosuccinate synthase OS=Desulfotomaculum reducens (strain MI-1) OX=349161 ... [more]
Match NameE-valueIdentityDescription
A0A5D3BHJ52.8e-24789.27Citrulline--aspartate ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3AZP12.8e-24789.27Citrulline--aspartate ligase OS=Cucumis melo OX=3656 GN=LOC103484309 PE=3 SV=1[more]
A0A0A0LWM95.2e-24688.46Citrulline--aspartate ligase OS=Cucumis sativus OX=3659 GN=Csa_1G050400 PE=3 SV=... [more]
A0A6J1E7A93.5e-24287.02Citrulline--aspartate ligase OS=Cucurbita moschata OX=3662 GN=LOC111431281 PE=3 ... [more]
A0A6J1KF743.9e-24186.61Citrulline--aspartate ligase OS=Cucurbita maxima OX=3661 GN=LOC111495262 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT4G24830.17.8e-21080.67arginosuccinate synthase family [more]
AT4G24830.22.2e-18071.91arginosuccinate synthase family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001518Argininosuccinate synthasePFAMPF00764Arginosuc_synthcoord: 53..439
e-value: 6.8E-159
score: 529.3
IPR001518Argininosuccinate synthaseTIGRFAMTIGR00032TIGR00032coord: 51..442
e-value: 1.7E-150
score: 499.7
IPR001518Argininosuccinate synthasePANTHERPTHR11587ARGININOSUCCINATE SYNTHASEcoord: 48..444
NoneNo IPR availableGENE3D1.20.5.470Single helix bincoord: 421..446
e-value: 1.5E-8
score: 35.8
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 50..214
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 51..220
e-value: 1.2E-164
score: 549.4
IPR024074Argininosuccinate synthetase, catalytic/multimerisation domain bodyGENE3D3.90.1260.10Argininosuccinate synthetase, chain A, domain 2coord: 111..401
e-value: 1.2E-164
score: 549.4
IPR024074Argininosuccinate synthetase, catalytic/multimerisation domain bodySUPERFAMILY69864Argininosuccinate synthetase, C-terminal domaincoord: 219..446
IPR018223Argininosuccinate synthase, conserved sitePROSITEPS00564ARGININOSUCCIN_SYN_1coord: 55..63
IPR018223Argininosuccinate synthase, conserved sitePROSITEPS00565ARGININOSUCCIN_SYN_2coord: 164..175
IPR023434Argininosuccinate synthase, type 1 subfamilyHAMAPMF_00005Arg_succ_synth_type1coord: 49..445
score: 53.7425
IPR023434Argininosuccinate synthase, type 1 subfamilyCDDcd01999Argininosuccinate_Synthasecoord: 52..436
e-value: 0.0
score: 664.233

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020957.1HG10020957.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006526 arginine biosynthetic process
biological_process GO:0000053 argininosuccinate metabolic process
biological_process GO:0000050 urea cycle
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004055 argininosuccinate synthase activity
molecular_function GO:0005524 ATP binding