HG10020891 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10020891
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	High-affinity nitrate transporter
Location	Chr05: 3304367 .. 3305099 (+)
RNA-Seq Expression	HG10020891
Synteny	HG10020891

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCAAATGGGTTTCTCTTGGCTGTTCTGTTTCTGTCCTGTTTCTTGGGATTCTCTCATGGAAATGGCCATTTCTCTTCTCTCCAAAGAACTTTGGAGGTCACTGCTTCACCAAAACCTGGACAAGGTAAGATTTTTCACCTCCCCCATCTCTCTTCCATGGAGATCTGAATAAAAAATTCAAACTTTACCAATTTTTAACAACTGGGTTGTTTTTTCTGCAGTTCTTAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTTTAAATGGGACTGTCAAAGCTGGGTCGGATTCGGGTTACAAGAACATCAAGGTGAAGCTCTGTTACGCGCCGGTGAGTCAAGTGGACCGAGCGTGGAGGAAAACAGTGGACGATCTCTCAAAGGACAAGACTTGTCAATTCGTCATCGTGGAAAAGCCGTACAATCCGGCGAACAAGACGGTGCAAAAGTTTGAGTGGACTGTGGAGCGTGACATTCCGACAGGGACTTTCTTTGTCAGAGCCTACGTTTTAAATTCCGCTGGCGTTGAAGTGGCGTACGGCCAAACGACTGATGATAAAAAAGGAACAAACTTGTTTCAAATTGAATCGATCACTGGTCGTCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTTGTGTCGTTGTTCGGATTCTTCTTCATCGACAAGAGGAAGGCCAAGAAGGCGGCGGCGGCGGCGCAGGGGCAGTAA

mRNA sequence

ATGGCTTCAAATGGGTTTCTCTTGGCTGTTCTGTTTCTGTCCTGTTTCTTGGGATTCTCTCATGGAAATGGCCATTTCTCTTCTCTCCAAAGAACTTTGGAGGTCACTGCTTCACCAAAACCTGGACAAGTTCTTAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTTTAAATGGGACTGTCAAAGCTGGGTCGGATTCGGGTTACAAGAACATCAAGGTGAAGCTCTGTTACGCGCCGGTGAGTCAAGTGGACCGAGCGTGGAGGAAAACAGTGGACGATCTCTCAAAGGACAAGACTTGTCAATTCGTCATCGTGGAAAAGCCGTACAATCCGGCGAACAAGACGGTGCAAAAGTTTGAGTGGACTGTGGAGCGTGACATTCCGACAGGGACTTTCTTTGTCAGAGCCTACGTTTTAAATTCCGCTGGCGTTGAAGTGGCGTACGGCCAAACGACTGATGATAAAAAAGGAACAAACTTGTTTCAAATTGAATCGATCACTGGTCGTCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTTGTGTCGTTGTTCGGATTCTTCTTCATCGACAAGAGGAAGGCCAAGAAGGCGGCGGCGGCGGCGCAGGGGCAGTAA

Coding sequence (CDS)

ATGGCTTCAAATGGGTTTCTCTTGGCTGTTCTGTTTCTGTCCTGTTTCTTGGGATTCTCTCATGGAAATGGCCATTTCTCTTCTCTCCAAAGAACTTTGGAGGTCACTGCTTCACCAAAACCTGGACAAGTTCTTAAAGCGGGTGTTGATAAAATCAGTGTGACCTGGGTTTTAAATGGGACTGTCAAAGCTGGGTCGGATTCGGGTTACAAGAACATCAAGGTGAAGCTCTGTTACGCGCCGGTGAGTCAAGTGGACCGAGCGTGGAGGAAAACAGTGGACGATCTCTCAAAGGACAAGACTTGTCAATTCGTCATCGTGGAAAAGCCGTACAATCCGGCGAACAAGACGGTGCAAAAGTTTGAGTGGACTGTGGAGCGTGACATTCCGACAGGGACTTTCTTTGTCAGAGCCTACGTTTTAAATTCCGCTGGCGTTGAAGTGGCGTACGGCCAAACGACTGATGATAAAAAAGGAACAAACTTGTTTCAAATTGAATCGATCACTGGTCGTCATATCTCACTTGATATTGCCTCTGTTTGCTTCAGTGCTTTCTCTGTTGTGTCGTTGTTCGGATTCTTCTTCATCGACAAGAGGAAGGCCAAGAAGGCGGCGGCGGCGGCGCAGGGGCAGTAA

Protein sequence

MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAAAQGQ

Homology

[Close]

BLAST of HG10020891 vs. NCBI nr
Match: NP_001267619.1 (high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus] >ACV33078.2 high-affinity nitrate transport system component [Cucumis sativus] >KGN64351.1 hypothetical protein Csa_013320 [Cucumis sativus])

HSP 1 Score: 383.3 bits (983), Expect = 1.4e-102
Identity = 194/211 (91.94%), Postives = 195/211 (92.42%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MASN FLLA LFLSCFLGFSHGN HFSSL RTLEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVKAGSDS YKNIK KLCYAPVSQVDRAWRKT DDL KDKTCQF IVEK YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTV+RDIPTGTFFVRAYVLNSAG EVAYGQTTD KKGTNLFQIESITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQGQ 212
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA GQ
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

[Close]

BLAST of HG10020891 vs. NCBI nr
Match: XP_008439011.1 (PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa] >TYJ99190.1 high-affinity nitrate transporter 3.1-like [Cucumis melo var. makuwa])

HSP 1 Score: 382.1 bits (980), Expect = 3.1e-102
Identity = 191/209 (91.39%), Postives = 194/209 (92.82%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MASNGFLLA LFLSCFLGFS GN H SSL RTLEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVKAGSDS YKNIK KLCYAPVSQVDRAWRKT DDL KDKTCQF IVEKPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTVERD+PTGTFFVRAYV NSAG EVAYGQTTDDKKGTNLFQIESITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQ 210
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA+
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]

BLAST of HG10020891 vs. NCBI nr
Match: XP_038894835.1 (high-affinity nitrate transporter 3.1-like [Benincasa hispida])

HSP 1 Score: 379.4 bits (973), Expect = 2.0e-101
Identity = 191/211 (90.52%), Postives = 194/211 (91.94%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MASNGFLLA LFLSCFLGFSHGN H SSLQRTLEVTASPKPGQVLKAGVDKISVTW+  G
Sbjct: 1   MASNGFLLATLFLSCFLGFSHGNIHLSSLQRTLEVTASPKPGQVLKAGVDKISVTWIFVG 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           T K  SDS YKNIK KLCYAPVSQVDRAWRKT DDLSKDKTCQF IVEKPYNPANKTVQK
Sbjct: 61  TDKVKSDSDYKNIKAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFDIVEKPYNPANKTVQK 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTVERD+PT TFFVR YVLNSAG +VAYGQTTDDKKGTNLFQIESITGRHISLDIASV
Sbjct: 121 FEWTVERDVPTATFFVRVYVLNSAGDKVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQGQ 212
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA  Q
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAAAQ 211

[Close]

BLAST of HG10020891 vs. NCBI nr
Match: XP_023520111.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.1 bits (915), Expect = 1.1e-94
Identity = 176/209 (84.21%), Postives = 187/209 (89.47%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M++NGFLLA LFL CF GFSHG+ HFSSL R+LEVTASPKPGQVLKAGVDKISVTW+LNG
Sbjct: 7   MSANGFLLAALFLFCFFGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWILNG 66

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVK+GSDS YK ++ KLCYAPVSQVDRAWRKT DDLSKDKTCQF+IV KPYNPANKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPANKTVQK 126

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTV RDIPTGTFFVRAY  NSAG EV YGQTTD+KKGTNLFQIESITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGEEVGYGQTTDNKKGTNLFQIESITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQ 210
           CFSAFSV+SL GFF   KRKAKKA A  Q
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]

BLAST of HG10020891 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 355.9 bits (912), Expect = 2.4e-94
Identity = 176/209 (84.21%), Postives = 187/209 (89.47%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M++NGFLLA LFL CFLGFSHG+ HFSSL R+LEVTASPKPGQVLKAGVDKISVTWVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVK+GSDS YK ++ KLCYAPVSQVDRAWRKT DDLSKDKTCQF+IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTV RDIPTGTFFVRAY  NSAG EV YGQTTD+KKGTNLFQIE+ITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQ 210
           CFSAFSV+SL GFF   KRKAKKA A  Q
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]

BLAST of HG10020891 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 4.4e-51
Identity = 111/209 (53.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGH---FSSLQR-TLEVTASPK---PGQVLKAGVDKIS 60
           MA    L A L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHI 180
            +KT+Q   WT+ERDIPTGT+FVRAY +++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF  +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of HG10020891 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 2.0e-48
Identity = 104/207 (50.24%), Postives = 139/207 (67.15%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGH---FSSLQRTLEVTASP-KPGQVLKAGVDKISVTW 60
           MA +  L   L +   +  S G      F+ LQ ++EVTA P K   VL+AG D +++TW
Sbjct: 1   MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 60

Query: 61  VL-NGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPAN 120
            L + + K  +D+ +K I+VKLCYAP+SQVDR WRKT + L KD++C   IV K Y   +
Sbjct: 61  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 120

Query: 121 KTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISL 180
           KT Q  +WT+  DIPTGT+FVRAY ++  G EVAYGQ+TD+ + TNLF + +I+G H+ L
Sbjct: 121 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 180

Query: 181 DIASVCFSAFSVVSLFGFFFIDKRKAK 203
           DIAS  FS FSVVSLF FF ++KRKAK
Sbjct: 181 DIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]

BLAST of HG10020891 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.7e-42
Identity = 96/206 (46.60%), Postives = 125/206 (60.68%), Query Frame = 0


Query: 8   LAVLFLSCFLGFSH-------GNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 67
           L +L L   LG  H            S+L + L V ASP+ GQVL AG D I+VTW LN 
Sbjct: 13  LPLLLLLVVLGACHVTPAAAAAGARLSALAKALVVEASPRAGQVLHAGEDAITVTWSLNA 72

Query: 68  T---VKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKT 127
           T     AG+D+GYK +KV LCYAP SQV R WRK  DDLSKDK CQF I ++PY+ A   
Sbjct: 73  TAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRKAHDDLSKDKACQFKIAQQPYDGAG-- 132

Query: 128 VQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDI 187
             KFE+TV RD+PT +++VRAY L+++G  VAYG+T         F +  ITG   S+++
Sbjct: 133 --KFEYTVARDVPTASYYVRAYALDASGARVAYGETAPSAS----FAVAGITGVTASIEV 192

Query: 188 ASVCFSAFSVVSLFGFFFIDKRKAKK 204
           A+   SAFSV +L  F  ++ +K  K
Sbjct: 193 AAGVLSAFSVAALAVFLVLENKKKNK 210

[Close]

BLAST of HG10020891 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 1.4e-41
Identity = 84/177 (47.46%), Postives = 117/177 (66.10%), Query Frame = 0


Query: 27  SSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNGTVKAGSDSGYKNIKVKLCYAPVSQVD 86
           S L + L V  SPK G+V+ AG + ++VTW LN +  AG+D+ +K++KVKLCYAP S+ D
Sbjct: 37  SKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKVKLCYAPASRTD 96

Query: 87  RAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQKFEWTVERDIPTGTFFVRAYVLNSAGV 146
           R WRK  DDL KDK CQF +  +PY        +F++ V RDIPT ++FVRAY ++++G 
Sbjct: 97  RGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYFVRAYAVDASGT 156

Query: 147 EVAYGQTTDDKKGTNLFQIESITGRHISLDIASVCFSAFSVVSLFGFFFIDKRKAKK 204
           EVAYGQ++ D      F +  ITG H SL +A+  FS FS+ +L  FF ++KRK  K
Sbjct: 157 EVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFVVEKRKKDK 206

[Close]

BLAST of HG10020891 vs. ExPASy TrEMBL
Match: C8CHG8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2)

HSP 1 Score: 383.3 bits (983), Expect = 6.7e-103
Identity = 194/211 (91.94%), Postives = 195/211 (92.42%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MASN FLLA LFLSCFLGFSHGN HFSSL RTLEVTASPKPGQVLKAGVDKISVTWVLN 
Sbjct: 1   MASNAFLLAALFLSCFLGFSHGNIHFSSLPRTLEVTASPKPGQVLKAGVDKISVTWVLNE 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVKAGSDS YKNIK KLCYAPVSQVDRAWRKT DDL KDKTCQF IVEK YNPANKTVQ 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFSIVEKQYNPANKTVQS 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTV+RDIPTGTFFVRAYVLNSAG EVAYGQTTD KKGTNLFQIESITGRHISLDIAS 
Sbjct: 121 FEWTVKRDIPTGTFFVRAYVLNSAGEEVAYGQTTDAKKGTNLFQIESITGRHISLDIASA 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQGQ 212
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA GQ
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAPGQ 211

[Close]

BLAST of HG10020891 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.5e-102
Identity = 191/209 (91.39%), Postives = 194/209 (92.82%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MASNGFLLA LFLSCFLGFS GN H SSL RTLEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVKAGSDS YKNIK KLCYAPVSQVDRAWRKT DDL KDKTCQF IVEKPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTVERD+PTGTFFVRAYV NSAG EVAYGQTTDDKKGTNLFQIESITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQ 210
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA+
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]

BLAST of HG10020891 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.5e-102
Identity = 191/209 (91.39%), Postives = 194/209 (92.82%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MASNGFLLA LFLSCFLGFS GN H SSL RTLEVTASPKPGQVLKAGVDKI VTWVLNG
Sbjct: 1   MASNGFLLAALFLSCFLGFSLGNIHLSSLPRTLEVTASPKPGQVLKAGVDKIRVTWVLNG 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVKAGSDS YKNIK KLCYAPVSQVDRAWRKT DDL KDKTCQF IVEKPYNPANKT Q 
Sbjct: 61  TVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTEDDLKKDKTCQFDIVEKPYNPANKTAQS 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTVERD+PTGTFFVRAYV NSAG EVAYGQTTDDKKGTNLFQIESITGRH+SLDIASV
Sbjct: 121 FEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTTDDKKGTNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQ 210
           CFSAFSVVSLFGFFFIDKRKAKKAAAAA+
Sbjct: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAE 209

[Close]

BLAST of HG10020891 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.2e-94
Identity = 176/209 (84.21%), Postives = 187/209 (89.47%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           M++NGFLLA LFL CFLGFSHG+ HFSSL R+LEVTASPKPGQVLKAGVDKISVTWVLNG
Sbjct: 7   MSANGFLLAALFLFCFLGFSHGHIHFSSLPRSLEVTASPKPGQVLKAGVDKISVTWVLNG 66

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TVK+GSDS YK ++ KLCYAPVSQVDRAWRKT DDLSKDKTCQF+IV KPYNP NKTVQK
Sbjct: 67  TVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTEDDLSKDKTCQFLIVGKPYNPGNKTVQK 126

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FEWTV RDIPTGTFFVRAY  NSAG EV YGQTTD+KKGTNLFQIE+ITGRHISLDIASV
Sbjct: 127 FEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTTDNKKGTNLFQIEAITGRHISLDIASV 186

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQ 210
           CFSAFSV+SL GFF   KRKAKKA A  Q
Sbjct: 187 CFSAFSVLSLSGFFLAGKRKAKKAEAPGQ 215

[Close]

BLAST of HG10020891 vs. ExPASy TrEMBL
Match: A0A6J1C949 (High-affinity nitrate transporter OS=Momordica charantia OX=3673 GN=LOC111009098 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.4e-94
Identity = 178/211 (84.36%), Postives = 189/211 (89.57%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGHFSSLQRTLEVTASPKPGQVLKAGVDKISVTWVLNG 60
           MA  GFLLA LFLSCF GFSHG GHFS+L RTLEVTASPKPGQVLKAGVDKI+V+WVLNG
Sbjct: 1   MAPRGFLLAALFLSCFFGFSHGKGHFSTLPRTLEVTASPKPGQVLKAGVDKINVSWVLNG 60

Query: 61  TVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQK 120
           TV+AGSDS YK +KVKLCYA VSQVDRAWRKT DDLSKDKTCQF+IVEKPYNPA   VQK
Sbjct: 61  TVRAGSDSEYKTVKVKLCYAKVSQVDRAWRKTEDDLSKDKTCQFLIVEKPYNPA---VQK 120

Query: 121 FEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASV 180
           FE TV+RD+PT TFFVRAY LNSAG EVAYGQTTDD+K TNLFQIESITGRH+SLDIASV
Sbjct: 121 FESTVKRDVPTATFFVRAYALNSAGDEVAYGQTTDDRKATNLFQIESITGRHVSLDIASV 180

Query: 181 CFSAFSVVSLFGFFFIDKRKAKKAAAAAQGQ 212
           CFSAFSVV+LFGFFF DKRKAK AAAAA  Q
Sbjct: 181 CFSAFSVVALFGFFFADKRKAKSAAAAAAAQ 208

[Close]

BLAST of HG10020891 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 202.6 bits (514), Expect = 3.1e-52
Identity = 111/209 (53.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGH---FSSLQR-TLEVTASPK---PGQVLKAGVDKIS 60
           MA    L A L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHI 180
            +KT+Q   WT+ERDIPTGT+FVRAY +++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF  +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of HG10020891 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 202.6 bits (514), Expect = 3.1e-52
Identity = 111/209 (53.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGH---FSSLQR-TLEVTASPK---PGQVLKAGVDKIS 60
           MA    L A L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHI 180
            +KT+Q   WT+ERDIPTGT+FVRAY +++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF  +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of HG10020891 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 202.6 bits (514), Expect = 3.1e-52
Identity = 111/209 (53.11%), Postives = 142/209 (67.94%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGH---FSSLQR-TLEVTASPK---PGQVLKAGVDKIS 60
           MA    L A L +   +   HG      F  L +  L+VT  P    PG VL AG D ++
Sbjct: 1   MAIQKILFASLLICSLIQSIHGAEKVRLFKELDKGALDVTTKPSREGPGVVLDAGKDTLN 60

Query: 61  VTWVLNGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNP 120
           +TW L+ ++ +  ++ +K IKVKLCYAP SQVDR WRKT D+L KDKTC   I+ KPY  
Sbjct: 61  ITWTLS-SIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKDKTCPHKIIAKPY-- 120

Query: 121 ANKTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHI 180
            +KT+Q   WT+ERDIPTGT+FVRAY +++ G EVAYGQ+TDD K TNLF +++I+GRH 
Sbjct: 121 -DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKKTNLFSVQAISGRHA 180

Query: 181 SLDIASVCFSAFSVVSLFGFFFIDKRKAK 203
           SLDIAS+CFS FSVV+L  FF  +KRKAK
Sbjct: 181 SLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of HG10020891 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.5e-49
Identity = 104/207 (50.24%), Postives = 139/207 (67.15%), Query Frame = 0


Query: 1   MASNGFLLAVLFLSCFLGFSHGNGH---FSSLQRTLEVTASP-KPGQVLKAGVDKISVTW 60
           MA +  L   L +   +  S G      F+ LQ ++EVTA P K   VL+AG D +++TW
Sbjct: 23  MAIHTLLFVSLLIFSLIESSSGGKKDRLFTDLQNSIEVTAKPVKDSGVLEAGKDMVTITW 82

Query: 61  VL-NGTVKAGSDSGYKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPAN 120
            L + + K  +D+ +K I+VKLCYAP+SQVDR WRKT + L KD++C   IV K Y   +
Sbjct: 83  KLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---D 142

Query: 121 KTVQKFEWTVERDIPTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISL 180
           KT Q  +WT+  DIPTGT+FVRAY ++  G EVAYGQ+TD+ + TNLF + +I+G H+ L
Sbjct: 143 KTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGL 202

Query: 181 DIASVCFSAFSVVSLFGFFFIDKRKAK 203
           DIAS  FS FSVVSLF FF ++KRKAK
Sbjct: 203 DIASTFFSVFSVVSLFVFFVMEKRKAK 226

[Close]

BLAST of HG10020891 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 187.6 bits (475), Expect = 1.0e-47
Identity = 98/193 (50.78%), Postives = 129/193 (66.84%), Query Frame = 0


Query: 15  CFLGFSHGNGHFSSL----QRTLEVTASPKPGQVLKAGVDKISVTWVL-NGTVKAGSDSG 74
           C  G  H  G+F        R+LE      P  VL+AG D +++TW L + + K  +D+ 
Sbjct: 251 CLSGHDHKGGYFVDSHGVHHRSLEAALECPP--VLEAGKDMVTITWKLKSSSAKVDTDTA 310

Query: 75  YKNIKVKLCYAPVSQVDRAWRKTVDDLSKDKTCQFVIVEKPYNPANKTVQKFEWTVERDI 134
           +K I+VKLCYAP+SQVDR WRKT + L KD++C   IV K Y   +KT Q  +WT+  DI
Sbjct: 311 FKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDI 370

Query: 135 PTGTFFVRAYVLNSAGVEVAYGQTTDDKKGTNLFQIESITGRHISLDIASVCFSAFSVVS 194
           PTGT+FVRAY ++  G EVAYGQ+TD+ + TNLF + +I+G H+ LDIAS  FS FSVVS
Sbjct: 371 PTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVS 430

Query: 195 LFGFFFIDKRKAK 203
           LF FF ++KRKAK
Sbjct: 431 LFVFFVMEKRKAK 438

The following BLAST results are available for this feature:

BLAST of HG10020891 vs. NCBI nr


Match Name	E-value	Identity	Description
NP_001267619.1	1.4e-102	91.94	high-affinity nitrate transporter 3.1-like precursor [Cucumis sativus] >ACV33078...	[more]
XP_008439011.1	3.1e-102	91.39	PREDICTED: high-affinity nitrate transporter 3.1-like [Cucumis melo] >KAA0055266...	[more]
XP_038894835.1	2.0e-101	90.52	high-affinity nitrate transporter 3.1-like [Benincasa hispida]	[more]
XP_023520111.1	1.1e-94	84.21	high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022924070.1	2.4e-94	84.21	high-affinity nitrate transporter 3.1 [Cucurbita moschata]	[more]

BLAST of HG10020891 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGS5	4.4e-51	53.11	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ...	[more]
Q9SB67	2.0e-48	50.24	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ...	[more]
Q7XK12	3.7e-42	46.60	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]
Q6ZI50	1.4e-41	47.46	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]

BLAST of HG10020891 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
C8CHG8	6.7e-103	91.94	High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NAR2 PE=2 SV=2	[more]
A0A5A7ULV7	1.5e-102	91.39	High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3AXC1	1.5e-102	91.39	High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S...	[more]
A0A6J1EDS1	1.2e-94	84.21	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ...	[more]
A0A6J1C949	4.4e-94	84.36	High-affinity nitrate transporter OS=Momordica charantia OX=3673 GN=LOC111009098...	[more]

BLAST of HG10020891 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50200.3	3.1e-52	53.11	nitrate transmembrane transporters	[more]
AT5G50200.2	3.1e-52	53.11	nitrate transmembrane transporters	[more]
AT5G50200.1	3.1e-52	53.11	nitrate transmembrane transporters	[more]
AT4G24730.4	1.5e-49	50.24	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]
AT4G24730.1	1.0e-47	50.78	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 26..201 e-value: 9.3E-78 score: 259.6
IPR016605	High-affinity nitrate transporter	PIRSF	PIRSF012939	NAR2	coord: 1..205 e-value: 1.3E-85 score: 283.7
IPR016605	High-affinity nitrate transporter	PANTHER	PTHR34806	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 3..204
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 3..204

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10020891.1	HG10020891.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity