HG10020754 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10020754
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr05: 2151474 .. 2153927 (+)
RNA-Seq Expression	HG10020754
Synteny	HG10020754

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCACAGCAAATAATAATGTTGAGAAACAACACAAAGAGGAAAATAGAAAACAGAGGGTTGAACATATTATTGGAAGAAGATGCAATCAACGAGATTCTTTCCTTTGCCTCCGACTTCCCTGGCCATGAGGACGACGCCGTAGATCTTCTGCTCGATATCCTCGCAATGGAGCATACATCAAGTACTATTATATATTTTTTTAATTTTTTTATTATTAAAAAATTATATTTAAACGTGTAAAATTAAAAATTAAATCAATTTTAAGTGATTAAAATATATTTTTTTTAGTGATTTTAAAAATGACAAAAGTGATTTTAACTATTTTTAAAATCACTCCCAAACATAACCTTAAAACCCTTAAATGAATCAATTTCATTAGTAGAGTCTAGTTTATCCCTTTTAAAAGGTTTAATAGGTCATTCTATTTTAAAAATTGGCTAATAAATTCTAAAAACTTTAAAAAAAATATATAATAAATATAACTTTCAATTTTGATCCAATAAATCTTTAAAAATTTTAATTTTGTATCTAATAGGTCATTAACCTACCTAACATATTAAATTGAGATTTTAGAATTCCATTAGATATAAAATTGACTCTATATCTAATATATCAATTATCAATTGATTTTTTTTAAAAAAAAATTGAATAGATCAGAGACTTATTAAATACAAAATTAATAGTTTATAGAATTATTAGACATAAAATTTTAAAATATATGAACCTATTGGGTACTATTGAAAGTTAAAGGATTTATTAGATGTTTTTTTTCTTTGTAACATCAAACTAAATAAACACAAAATAAATTTATAGAATGAACTTGTAATTTACTATATAATGTACCAATTTCAATTTCAAATAGCAACAATTGTTGAAGAAAAAATAAAATAAAAAGAACTCTTCGTTTTATATCTTTTATTATCAACCGTTAATTATTAGTTTTGGGTACATTTTTTTTCCTCCTTTTTTCATATATTGCAATTCTCATCACAATTGTTTTTACTATTTATGATCACATTATTTCAGCAATTGATCACATTATTCGTTTCTGTTGTGGGTAAATTCTTTGTTGATTTGCTGGTTTCCTTGGCAGATCTAGCTCCAATAATAGATATTGATTTACTTCGTTGGACAGTAAATCGTTTACGTGCTGCGCAAAGTGCGAGTTCAACACAGCAGGTGATTTCTTTTTTAATTATTATTTTTTAGTTTAGTACATGCTCAAAATTAGGAGTGCAACTAGGTGTGTGCACGATTTAATTTGACCGGACCGAAGTGCGGAATCGTCGAAAATCGGCTTACCTCAATTTCGATTTTTGAACCGAAATCAGGTTGGTTCGGTTCGATTTAAAACCTTTCCTTTTTCTTTTTTTTCCTTCCTCTTCTGCTTTATGCCACAACTCATTTTTTTACTCTTTCTTGTTACAAAAATCTTAATCAAACATCTAAAGCAACTTAAAATTTAATACAATTCATCCAAAGTCTACCAAACTTTCAAATCATCCTGAAATTTAAAGTAATTCATACAATCAAAGTTAAAACATAAAATACAATCTATCCATTCAGAGTCTAAATACTAGAAATTTAAATATAAAATACAGTCCGTCTAAAAAATTCAAACACTAACATAAAACGAAATTGAAGTACATACAAATTACAAAGTTTGAAGTTCAAACACAACAAAATGATTACAAGCAAACCAAGCACAGGTTCCTCCAACTCCATTATTCAATCACCTAAATTAACTGTGTTTATAAAATAAACAAATTAGTTTATAAAATATAAATAAAAAGATATTAAAAAAAGTATACTTAGGATTTTTTAATGGAAAATTTTCATAGATAAAAAAAATATCAAACTATTTATAGAAAATAACAAAAAAAAAAATACTGATAGATTTCTATCAACCTCTATGTTTTTATCGACTTCCATCCCAATTATATTTTGTGTAAATAGTTTTCCTTATTTTTTTATTTTTAAAATTTTCCTTTTTTAATTCTAATTTTTCTATTTTCTAATTCTAAATTTCTAATTAGTTTTATTTTCCTCGGAAACAATCTCCAGATCGTCTATTTTGAATCCGGTAATATTGAAATGGGCGAAAATCATCAAGACGCCTCCGATTTGGATTTTTGGGACTCCTTGTTCGATCCCGACGGGGAGGCGTGGATAGAACAACTTTCTGAAAACGATCTGAGCCGGTACGAAAGTTTCCCGTTCCCGGCTTCGAGATCGGCGATTGAGGCACTACCAAATATTTTTACTACGGACGATTCTTCTTATTCTTCTTCTTCTTGGAATTGTGCTGTGTGTATGGAAGACAAGATTGAGGCAAATGAAGGGGCAAAACAGATGCCTTGTAAACATATATTTCATACACATTGTATAATTCCTTGGTTGGAATTGCATAATTCTTGCCCACTCTGTCGATATCAATTGCCCACATAG

mRNA sequence

ATGGAATCACAGCAAATAATAATGTTGAGAAACAACACAAAGAGGAAAATAGAAAACAGAGGGTTGAACATATTATTGGAAGAAGATGCAATCAACGAGATTCTTTCCTTTGCCTCCGACTTCCCTGGCCATGAGGACGACGCCGTAGATCTTCTGCTCGATATCCTCGCAATGGAGCATACATCAAATCTAGCTCCAATAATAGATATTGATTTACTTCGTTGGACAGTAAATCGTTTACGTGCTGCGCAAAGTGCGAGTTCAACACAGCAGATCGTCTATTTTGAATCCGGTAATATTGAAATGGGCGAAAATCATCAAGACGCCTCCGATTTGGATTTTTGGGACTCCTTGTTCGATCCCGACGGGGAGGCGTGGATAGAACAACTTTCTGAAAACGATCTGAGCCGGTACGAAAGTTTCCCGTTCCCGGCTTCGAGATCGGCGATTGAGGCACTACCAAATATTTTTACTACGGACGATTCTTCTTATTCTTCTTCTTCTTGGAATTGTGCTGTGTGTATGGAAGACAAGATTGAGGCAAATGAAGGGGCAAAACAGATGCCTTGTAAACATATATTTCATACACATTGTATAATTCCTTGGTTGGAATTGCATAATTCTTGCCCACTCTGTCGATATCAATTGCCCACATAG

Coding sequence (CDS)

ATGGAATCACAGCAAATAATAATGTTGAGAAACAACACAAAGAGGAAAATAGAAAACAGAGGGTTGAACATATTATTGGAAGAAGATGCAATCAACGAGATTCTTTCCTTTGCCTCCGACTTCCCTGGCCATGAGGACGACGCCGTAGATCTTCTGCTCGATATCCTCGCAATGGAGCATACATCAAATCTAGCTCCAATAATAGATATTGATTTACTTCGTTGGACAGTAAATCGTTTACGTGCTGCGCAAAGTGCGAGTTCAACACAGCAGATCGTCTATTTTGAATCCGGTAATATTGAAATGGGCGAAAATCATCAAGACGCCTCCGATTTGGATTTTTGGGACTCCTTGTTCGATCCCGACGGGGAGGCGTGGATAGAACAACTTTCTGAAAACGATCTGAGCCGGTACGAAAGTTTCCCGTTCCCGGCTTCGAGATCGGCGATTGAGGCACTACCAAATATTTTTACTACGGACGATTCTTCTTATTCTTCTTCTTCTTGGAATTGTGCTGTGTGTATGGAAGACAAGATTGAGGCAAATGAAGGGGCAAAACAGATGCCTTGTAAACATATATTTCATACACATTGTATAATTCCTTGGTTGGAATTGCATAATTCTTGCCCACTCTGTCGATATCAATTGCCCACATAG

Protein sequence

MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEHTSNLAPIIDIDLLRWTVNRLRAAQSASSTQQIVYFESGNIEMGENHQDASDLDFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT

Homology

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BLAST of HG10020754 vs. NCBI nr
Match: XP_008446033.1 (PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucumis melo])

HSP 1 Score: 270.8 bits (691), Expect = 1.0e-68
Identity = 140/222 (63.06%), Postives = 171/222 (77.03%), Query Frame = 0


Query: 1   MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEH 60
           ME+QQII LR NTKRKIE+RG+N LLEE+A++EILSFASDFP HED+AVDLLLD++  E 
Sbjct: 1   MEAQQIITLRTNTKRKIESRGMNRLLEEEAVDEILSFASDFPSHEDEAVDLLLDVITTEL 60

Query: 61  TSNLAPIIDIDLLRWTVNRLRAAQ--SASSTQQIVYFESGNIEMGENHQDASDLDFWDSL 120
           TSN A +ID+DL+ W V  LRAA   SA ST Q   FES  +E  E+  D +D +  D L
Sbjct: 61  TSNRARVIDMDLVHWAVTLLRAANNTSADSTPQDANFESDILETRESIVDLTDSNVTDLL 120

Query: 121 FDPDGEAW--IEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCME 180
            D D + W  + QLS +DL R+ SFPF AS+SAI+ALP++F TDD    SSSW CAVCM+
Sbjct: 121 DDDDVDIWMHVRQLSLDDLIRHASFPFGASKSAIQALPDVFPTDD---FSSSWACAVCMD 180

Query: 181 DKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           D+I+A +GAKQ+PC+HIFHTHCII WLELHNSCPLCRY+LPT
Sbjct: 181 DEIDAKDGAKQLPCEHIFHTHCIISWLELHNSCPLCRYKLPT 219

[Close]

BLAST of HG10020754 vs. NCBI nr
Match: KGN64205.1 (hypothetical protein Csa_014029 [Cucumis sativus])

HSP 1 Score: 259.6 bits (662), Expect = 2.4e-65
Identity = 138/228 (60.53%), Postives = 164/228 (71.93%), Query Frame = 0


Query: 1   MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEH 60
           ME+QQ   L+NNT+RKIE RG+N LLEE+A++EI+SFASDFP HEDDAVDLLLD +  EH
Sbjct: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDDAVDLLLDFITNEH 60

Query: 61  TSNLAPIIDIDLLRWTVNRLRAA-------QSASSTQQIVYFESGNIEMGENHQDASDLD 120
           TSN A  ID++L+ W +N LRAA        S SSTQQ  Y ES  +E  E   D +D +
Sbjct: 61  TSNGALAIDMNLVHWAINHLRAADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTDSN 120

Query: 121 FWDSLFDPDGEAW---IEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWN 180
             D L   D E W   + QLS NDLS +E FPF AS+S I+ALP++FT  D    SSSW 
Sbjct: 121 --DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKD---FSSSWV 180

Query: 181 CAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           C VC+ D+IEAN+GAKQ+PCKHIFHTHCII WLEL NSCPLCRYQLPT
Sbjct: 181 CGVCLNDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 223

[Close]

BLAST of HG10020754 vs. NCBI nr
Match: TYJ99062.1 (pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 158.3 bits (399), Expect = 7.5e-35
Identity = 96/197 (48.73%), Postives = 120/197 (60.91%), Query Frame = 0


Query: 1   MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEH 60
           ME+QQII LR NTKRKIE+RG+N LLEE+A++EILSFASDFP HED+AVDLLLD      
Sbjct: 1   MEAQQIITLRTNTKRKIESRGMNRLLEEEAVDEILSFASDFPSHEDEAVDLLLD------ 60

Query: 61  TSNLAPIIDIDLLRWTVNRLRAAQSASSTQQIVYFESGNIEMGENHQDASDLDFWDSLFD 120
                                             FES  +E  E+  D +D +  D L D
Sbjct: 61  -------------------------------DANFESDILETRESIVDLTDSNVTDLLDD 120

Query: 121 PDGEAW--IEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCMEDK 180
            D + W  + QLS +DL R+ SFPF AS+SAI+ALP++F TDD    SSSW CAVCM+D+
Sbjct: 121 DDVDIWMHVRQLSLDDLIRHASFPFGASKSAIQALPDVFPTDD---FSSSWACAVCMDDE 157

Query: 181 IEANEGAKQMPCKHIFH 196
           I+A +GAKQ+P   I H
Sbjct: 181 IDAKDGAKQLPSLRISH 157

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BLAST of HG10020754 vs. NCBI nr
Match: XP_023001793.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima] >XP_023001794.1 E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima])

HSP 1 Score: 131.7 bits (330), Expect = 7.5e-27
Identity = 71/131 (54.20%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 94  YFESGNIEM------GENHQDASDLDFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASR 153
           Y +S N+++      GE    +S+ +  D  F P  E  I+QL+END +RY +   PAS+
Sbjct: 147 YLQSANVQLVIQNASGEAFHPSSNFNLGDYFFGPGLEQLIQQLAENDPNRYGT--PPASK 206

Query: 154 SAIEALPNIFTTDDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHN 213
           SAIEALPNI  T++   S SS  CAVC +D  E NE AKQMPCKHI+H  CIIPWLELHN
Sbjct: 207 SAIEALPNIKITEELLASDSS-QCAVC-KDTFELNEEAKQMPCKHIYHADCIIPWLELHN 266

Query: 214 SCPLCRYQLPT 219
           SCP+CRY+LPT
Sbjct: 267 SCPVCRYELPT 273

[Close]

BLAST of HG10020754 vs. NCBI nr
Match: XP_022994940.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima] >XP_022994941.1 E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima] >XP_022994942.1 E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima] >XP_022994943.1 E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima])

HSP 1 Score: 131.7 bits (330), Expect = 7.5e-27
Identity = 71/131 (54.20%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 94  YFESGNIEM------GENHQDASDLDFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASR 153
           Y +S N+++      GE    +S+ +  D  F P  E  I+QL+END +RY +   PAS+
Sbjct: 143 YLQSANVQLVIQNASGEAFHPSSNFNLGDYFFGPGLEQLIQQLAENDPNRYGT--PPASK 202

Query: 154 SAIEALPNIFTTDDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHN 213
           SAIEALPNI  T++   S SS  CAVC +D  E NE AKQMPCKHI+H  CIIPWLELHN
Sbjct: 203 SAIEALPNIKITEELLASDSS-QCAVC-KDTFELNEEAKQMPCKHIYHADCIIPWLELHN 262

Query: 214 SCPLCRYQLPT 219
           SCP+CRY+LPT
Sbjct: 263 SCPVCRYELPT 269

[Close]

BLAST of HG10020754 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.6e-24
Identity = 57/106 (53.77%), Postives = 73/106 (68.87%), Query Frame = 0


Query: 113 DFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCA 172
           +F D  F P  E  I+QL+END +RY +   PAS+SAI+ALP +  T D   S  +  CA
Sbjct: 161 NFGDYFFGPGLEQLIQQLAENDPNRYGT--PPASKSAIDALPTVKVTKDMLKSEMN-QCA 220

Query: 173 VCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           VCM D+ E     KQMPCKH+FH  C++PWLELHNSCP+CR++LPT
Sbjct: 221 VCM-DEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of HG10020754 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 3.1e-23
Identity = 57/103 (55.34%), Postives = 73/103 (70.87%), Query Frame = 0


Query: 116 DSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCM 175
           D    P  E  I+QL+END +RY +   PAS+SAIEALP +  T  S+ +S    CAVCM
Sbjct: 172 DYFIGPGLEQLIQQLAENDPNRYGT--PPASKSAIEALPLVNIT-KSNLNSEFNQCAVCM 231

Query: 176 EDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           +D  E  E AKQMPCKH++H  C++PWLELHNSCP+CR++LPT
Sbjct: 232 DDFEEGTE-AKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPT 270

[Close]

BLAST of HG10020754 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.7e-18
Identity = 52/103 (50.49%), Postives = 61/103 (59.22%), Query Frame = 0


Query: 116 DSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCM 175
           D  F P  E   EQLS     R    P PA RSAI+ALP I        SS S NC VC 
Sbjct: 139 DYFFGPGLEELFEQLSAGTTRR---GPPPAPRSAIDALPTIKIAQRHLRSSDS-NCPVC- 198

Query: 176 EDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           +D+ E    AKQMPC HI+H+ CI+PWL  HNSCP+CR +LP+
Sbjct: 199 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS 236

[Close]

BLAST of HG10020754 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 5.1e-18
Identity = 50/103 (48.54%), Postives = 63/103 (61.17%), Query Frame = 0


Query: 116 DSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCM 175
           D  F P  E  IEQLS      +   P PA +S+I+ALP I  T     SS S +C VC 
Sbjct: 135 DYFFGPGLEELIEQLSS---GTHHRGPPPAPKSSIDALPTIKITQKHLKSSDS-HCPVC- 194

Query: 176 EDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           +D+ E    AKQMPC HI+H+ CI+PWL  HNSCP+CR +LP+
Sbjct: 195 KDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPS 232

[Close]

BLAST of HG10020754 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.3e-17
Identity = 46/98 (46.94%), Postives = 63/98 (64.29%), Query Frame = 0


Query: 124 EAWIEQLSENDLSRY---ESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCMEDKIE 183
           E  +EQLS+ + S      S   PAS+SAIE+LP +  +D   +  S  NCAVC E   E
Sbjct: 151 ERLLEQLSQIEASATGIGRSGNPPASKSAIESLPRVEISD--CHIGSEANCAVCTE-IFE 210

Query: 184 ANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
               A++MPCKH+FH  CI+PWL + NSCP+CR++LP+
Sbjct: 211 TETEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPS 245

[Close]

BLAST of HG10020754 vs. ExPASy TrEMBL
Match: A0A1S3BES6 (ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucumis melo OX=3656 GN=LOC103488881 PE=4 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 5.0e-69
Identity = 140/222 (63.06%), Postives = 171/222 (77.03%), Query Frame = 0


Query: 1   MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEH 60
           ME+QQII LR NTKRKIE+RG+N LLEE+A++EILSFASDFP HED+AVDLLLD++  E 
Sbjct: 1   MEAQQIITLRTNTKRKIESRGMNRLLEEEAVDEILSFASDFPSHEDEAVDLLLDVITTEL 60

Query: 61  TSNLAPIIDIDLLRWTVNRLRAAQ--SASSTQQIVYFESGNIEMGENHQDASDLDFWDSL 120
           TSN A +ID+DL+ W V  LRAA   SA ST Q   FES  +E  E+  D +D +  D L
Sbjct: 61  TSNRARVIDMDLVHWAVTLLRAANNTSADSTPQDANFESDILETRESIVDLTDSNVTDLL 120

Query: 121 FDPDGEAW--IEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCME 180
            D D + W  + QLS +DL R+ SFPF AS+SAI+ALP++F TDD    SSSW CAVCM+
Sbjct: 121 DDDDVDIWMHVRQLSLDDLIRHASFPFGASKSAIQALPDVFPTDD---FSSSWACAVCMD 180

Query: 181 DKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           D+I+A +GAKQ+PC+HIFHTHCII WLELHNSCPLCRY+LPT
Sbjct: 181 DEIDAKDGAKQLPCEHIFHTHCIISWLELHNSCPLCRYKLPT 219

[Close]

BLAST of HG10020754 vs. ExPASy TrEMBL
Match: A0A0A0LQL0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G043080 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 1.2e-65
Identity = 138/228 (60.53%), Postives = 164/228 (71.93%), Query Frame = 0


Query: 1   MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEH 60
           ME+QQ   L+NNT+RKIE RG+N LLEE+A++EI+SFASDFP HEDDAVDLLLD +  EH
Sbjct: 1   METQQRTTLKNNTERKIERRGMNRLLEEEAVDEIISFASDFPSHEDDAVDLLLDFITNEH 60

Query: 61  TSNLAPIIDIDLLRWTVNRLRAA-------QSASSTQQIVYFESGNIEMGENHQDASDLD 120
           TSN A  ID++L+ W +N LRAA        S SSTQQ  Y ES  +E  E   D +D +
Sbjct: 61  TSNGALAIDMNLVHWAINHLRAADAVVDVTNSVSSTQQNAYLESDILETREGVVDVTDSN 120

Query: 121 FWDSLFDPDGEAW---IEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWN 180
             D L   D E W   + QLS NDLS +E FPF AS+S I+ALP++FT  D    SSSW 
Sbjct: 121 --DLLDADDEEMWMAHVRQLSLNDLSLHEGFPFRASKSTIQALPDVFTAKD---FSSSWV 180

Query: 181 CAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           C VC+ D+IEAN+GAKQ+PCKHIFHTHCII WLEL NSCPLCRYQLPT
Sbjct: 181 CGVCLNDEIEANDGAKQLPCKHIFHTHCIISWLELCNSCPLCRYQLPT 223

[Close]

BLAST of HG10020754 vs. ExPASy TrEMBL
Match: A0A5D3BH26 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002660 PE=4 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 3.6e-35
Identity = 96/197 (48.73%), Postives = 120/197 (60.91%), Query Frame = 0


Query: 1   MESQQIIMLRNNTKRKIENRGLNILLEEDAINEILSFASDFPGHEDDAVDLLLDILAMEH 60
           ME+QQII LR NTKRKIE+RG+N LLEE+A++EILSFASDFP HED+AVDLLLD      
Sbjct: 1   MEAQQIITLRTNTKRKIESRGMNRLLEEEAVDEILSFASDFPSHEDEAVDLLLD------ 60

Query: 61  TSNLAPIIDIDLLRWTVNRLRAAQSASSTQQIVYFESGNIEMGENHQDASDLDFWDSLFD 120
                                             FES  +E  E+  D +D +  D L D
Sbjct: 61  -------------------------------DANFESDILETRESIVDLTDSNVTDLLDD 120

Query: 121 PDGEAW--IEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCMEDK 180
            D + W  + QLS +DL R+ SFPF AS+SAI+ALP++F TDD    SSSW CAVCM+D+
Sbjct: 121 DDVDIWMHVRQLSLDDLIRHASFPFGASKSAIQALPDVFPTDD---FSSSWACAVCMDDE 157

Query: 181 IEANEGAKQMPCKHIFH 196
           I+A +GAKQ+P   I H
Sbjct: 181 IDAKDGAKQLPSLRISH 157

[Close]

BLAST of HG10020754 vs. ExPASy TrEMBL
Match: A0A6J1KNN7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495829 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 3.6e-27
Identity = 71/131 (54.20%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 94  YFESGNIEM------GENHQDASDLDFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASR 153
           Y +S N+++      GE    +S+ +  D  F P  E  I+QL+END +RY +   PAS+
Sbjct: 147 YLQSANVQLVIQNASGEAFHPSSNFNLGDYFFGPGLEQLIQQLAENDPNRYGT--PPASK 206

Query: 154 SAIEALPNIFTTDDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHN 213
           SAIEALPNI  T++   S SS  CAVC +D  E NE AKQMPCKHI+H  CIIPWLELHN
Sbjct: 207 SAIEALPNIKITEELLASDSS-QCAVC-KDTFELNEEAKQMPCKHIYHADCIIPWLELHN 266

Query: 214 SCPLCRYQLPT 219
           SCP+CRY+LPT
Sbjct: 267 SCPVCRYELPT 273

[Close]

BLAST of HG10020754 vs. ExPASy TrEMBL
Match: A0A6J1K0N0 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111490530 PE=4 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 3.6e-27
Identity = 71/131 (54.20%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 94  YFESGNIEM------GENHQDASDLDFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASR 153
           Y +S N+++      GE    +S+ +  D  F P  E  I+QL+END +RY +   PAS+
Sbjct: 143 YLQSANVQLVIQNASGEAFHPSSNFNLGDYFFGPGLEQLIQQLAENDPNRYGT--PPASK 202

Query: 154 SAIEALPNIFTTDDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHN 213
           SAIEALPNI  T++   S SS  CAVC +D  E NE AKQMPCKHI+H  CIIPWLELHN
Sbjct: 203 SAIEALPNIKITEELLASDSS-QCAVC-KDTFELNEEAKQMPCKHIYHADCIIPWLELHN 262

Query: 214 SCPLCRYQLPT 219
           SCP+CRY+LPT
Sbjct: 263 SCPVCRYELPT 269

[Close]

BLAST of HG10020754 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 114.4 bits (285), Expect = 1.2e-25
Identity = 57/106 (53.77%), Postives = 73/106 (68.87%), Query Frame = 0


Query: 113 DFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCA 172
           +F D  F P  E  I+QL+END +RY +   PAS+SAI+ALP +  T D   S  +  CA
Sbjct: 161 NFGDYFFGPGLEQLIQQLAENDPNRYGT--PPASKSAIDALPTVKVTKDMLKSEMN-QCA 220

Query: 173 VCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           VCM D+ E     KQMPCKH+FH  C++PWLELHNSCP+CR++LPT
Sbjct: 221 VCM-DEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPT 262

[Close]

BLAST of HG10020754 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-20
Identity = 51/119 (42.86%), Postives = 70/119 (58.82%), Query Frame = 0


Query: 106 HQDASDL------DFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTT 165
           HQD+ D          D    P  E  +++L+ENDL+     P PA++ A+EAL  +   
Sbjct: 161 HQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTP-PATKEAVEALAMVKIE 220

Query: 166 DDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           D      S   C+VC++D  E    AK+MPCKH FH+ C++PWLELH+SCP+CRY LPT
Sbjct: 221 D------SLLQCSVCLDD-FEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPT 271

[Close]

BLAST of HG10020754 vs. TAIR 10
Match: AT3G13430.2 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-20
Identity = 51/119 (42.86%), Postives = 70/119 (58.82%), Query Frame = 0


Query: 106 HQDASDL------DFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTT 165
           HQD+ D          D    P  E  +++L+ENDL+     P PA++ A+EAL  +   
Sbjct: 161 HQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTP-PATKEAVEALAMVKIE 220

Query: 166 DDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           D      S   C+VC++D  E    AK+MPCKH FH+ C++PWLELH+SCP+CRY LPT
Sbjct: 221 D------SLLQCSVCLDD-FEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPT 271

[Close]

BLAST of HG10020754 vs. TAIR 10
Match: AT3G13430.3 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-20
Identity = 51/119 (42.86%), Postives = 70/119 (58.82%), Query Frame = 0


Query: 106 HQDASDL------DFWDSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTT 165
           HQD+ D          D    P  E  +++L+ENDL+     P PA++ A+EAL  +   
Sbjct: 161 HQDSVDTTSVPSGSLGDYFIGPGFETLLQRLAENDLNNRYGTP-PATKEAVEALAMVKIE 220

Query: 166 DDSSYSSSSWNCAVCMEDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           D      S   C+VC++D  E    AK+MPCKH FH+ C++PWLELH+SCP+CRY LPT
Sbjct: 221 D------SLLQCSVCLDD-FEIGMEAKEMPCKHKFHSDCLLPWLELHSSCPVCRYLLPT 271

[Close]

BLAST of HG10020754 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 94.4 bits (233), Expect = 1.2e-19
Identity = 52/103 (50.49%), Postives = 61/103 (59.22%), Query Frame = 0


Query: 116 DSLFDPDGEAWIEQLSENDLSRYESFPFPASRSAIEALPNIFTTDDSSYSSSSWNCAVCM 175
           D  F P  E   EQLS     R    P PA RSAI+ALP I        SS S NC VC 
Sbjct: 139 DYFFGPGLEELFEQLSAGTTRR---GPPPAPRSAIDALPTIKIAQRHLRSSDS-NCPVC- 198

Query: 176 EDKIEANEGAKQMPCKHIFHTHCIIPWLELHNSCPLCRYQLPT 219
           +D+ E    AKQMPC HI+H+ CI+PWL  HNSCP+CR +LP+
Sbjct: 199 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS 236

The following BLAST results are available for this feature:

BLAST of HG10020754 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008446033.1	1.0e-68	63.06	PREDICTED: ERAD-associated E3 ubiquitin-protein ligase HRD1-like [Cucumis melo]	[more]
KGN64205.1	2.4e-65	60.53	hypothetical protein Csa_014029 [Cucumis sativus]	[more]
TYJ99062.1	7.5e-35	48.73	pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa]	[more]
XP_023001793.1	7.5e-27	54.20	E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima] >XP_023001794.1 E3 ubi...	[more]
XP_022994940.1	7.5e-27	54.20	E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima] >XP_022994941.1 E3 ubi...	[more]

BLAST of HG10020754 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LPN7	1.6e-24	53.77	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	3.1e-23	55.34	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
O22197	1.7e-18	50.49	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	5.1e-18	48.54	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q940T5	3.3e-17	46.94	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of HG10020754 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BES6	5.0e-69	63.06	ERAD-associated E3 ubiquitin-protein ligase HRD1-like OS=Cucumis melo OX=3656 GN...	[more]
A0A0A0LQL0	1.2e-65	60.53	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G043080 P...	[more]
A0A5D3BH26	3.6e-35	48.73	Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A6J1KNN7	3.6e-27	54.20	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111495829 P...	[more]
A0A6J1K0N0	3.6e-27	54.20	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111490530 P...	[more]

BLAST of HG10020754 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19950.1	1.2e-25	53.77	RING/U-box superfamily protein	[more]
AT3G13430.1	3.3e-20	42.86	RING/U-box superfamily protein	[more]
AT3G13430.2	3.3e-20	42.86	RING/U-box superfamily protein	[more]
AT3G13430.3	3.3e-20	42.86	RING/U-box superfamily protein	[more]
AT2G40830.2	1.2e-19	50.49	RING-H2 finger C1A	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 171..212 e-value: 2.2E-7 score: 40.5
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 170..213 e-value: 2.8E-11 score: 43.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 171..213 score: 12.326995
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 133..218 e-value: 5.6E-21 score: 76.0
None	No IPR available	PANTHER	PTHR15710:SF127	SUBFAMILY NOT NAMED	coord: 131..218
None	No IPR available	PANTHER	PTHR15710:SF127	SUBFAMILY NOT NAMED	coord: 7..102
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 131..218
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 7..102
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 171..213 e-value: 3.77053E-21 score: 80.8089
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 156..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10020754.1	HG10020754.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity