HG10020626 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10020626
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	DNA polymerase alpha subunit B
Location	Chr05: 1050489 .. 1051034 (+)
RNA-Seq Expression	HG10020626
Synteny	HG10020626

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACAACAGCTTTTATCCTCTATACCCTCCAGCCGAAGGAGTTCCACTGGACTTTTCACTTGCTCCAAAAGCTCTCGACATCTCTGTAATACCTGATATTCTCATTCTTCCATCCGACATGAAACACTTCGTGAAGGTAAGTGATTTTGTCCTCATTATTTCATTACAATTTGCATAGTGTCAGTTTTGCCTTTGATCTTTCATCAATTTATTTATTCTCAAAACTACGTCAACAATGAACGTTAAAAATGGTGAGAATTGTGATACACGTTATAGATAAAGATAGACCAACCACTCAAGCTTAACCTCAACCTCTTATTTTCACCTTTAAGTAGATAAAACATACATGTTATTAAGAGTTTCAATTACCTCCCCACTATGTCAGGTTATATCCCTTGGTGAAGGAAGTTCAGGGGAAGAGCAAGCGAGATGCATATGTGTGAATCCTGGAAGATTGGCCAAGGGAGAAGGAGGAGGTACTTTTGTAGAGCTTAATTACTTCGGTAGGCCTGACCAGATAAATGCTTCAGTTGTCAGCATATGA

mRNA sequence

ATGCACAACAGCTTTTATCCTCTATACCCTCCAGCCGAAGGAGTTCCACTGGACTTTTCACTTGCTCCAAAAGCTCTCGACATCTCTGTAATACCTGATATTCTCATTCTTCCATCCGACATGAAACACTTCGTGAAGGTTATATCCCTTGGTGAAGGAAGTTCAGGGGAAGAGCAAGCGAGATGCATATGTGTGAATCCTGGAAGATTGGCCAAGGGAGAAGGAGGAGGTACTTTTGTAGAGCTTAATTACTTCGGTAGGCCTGACCAGATAAATGCTTCAGTTGTCAGCATATGA

Coding sequence (CDS)

ATGCACAACAGCTTTTATCCTCTATACCCTCCAGCCGAAGGAGTTCCACTGGACTTTTCACTTGCTCCAAAAGCTCTCGACATCTCTGTAATACCTGATATTCTCATTCTTCCATCCGACATGAAACACTTCGTGAAGGTTATATCCCTTGGTGAAGGAAGTTCAGGGGAAGAGCAAGCGAGATGCATATGTGTGAATCCTGGAAGATTGGCCAAGGGAGAAGGAGGAGGTACTTTTGTAGAGCTTAATTACTTCGGTAGGCCTGACCAGATAAATGCTTCAGTTGTCAGCATATGA

Protein sequence

MHNSFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCICVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI

Homology

[Close]

BLAST of HG10020626 vs. NCBI nr
Match: XP_038886670.1 (LOW QUALITY PROTEIN: DNA polymerase alpha subunit B [Benincasa hispida])

HSP 1 Score: 188.3 bits (477), Expect = 3.1e-44
Identity = 92/95 (96.84%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEG S EEQARCI
Sbjct: 529 SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCI 588

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPD+INASVVSI
Sbjct: 589 CVNPGRLAKGEGGGTFVELNYFGRPDKINASVVSI 623

[Close]

BLAST of HG10020626 vs. NCBI nr
Match: TYJ98951.1 (DNA polymerase alpha subunit B [Cucumis melo var. makuwa])

HSP 1 Score: 188.0 bits (476), Expect = 4.0e-44
Identity = 91/95 (95.79%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 549 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 608

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI
Sbjct: 609 CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 643

[Close]

BLAST of HG10020626 vs. NCBI nr
Match: XP_008452343.1 (PREDICTED: DNA polymerase alpha subunit B [Cucumis melo] >XP_008452395.1 PREDICTED: DNA polymerase alpha subunit B [Cucumis melo] >XP_008452468.1 PREDICTED: DNA polymerase alpha subunit B [Cucumis melo] >XP_008452549.1 PREDICTED: DNA polymerase alpha subunit B [Cucumis melo] >XP_008452631.1 PREDICTED: DNA polymerase alpha subunit B [Cucumis melo])

HSP 1 Score: 188.0 bits (476), Expect = 4.0e-44
Identity = 91/95 (95.79%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 528 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 587

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI
Sbjct: 588 CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 622

[Close]

BLAST of HG10020626 vs. NCBI nr
Match: XP_004137446.1 (DNA polymerase alpha subunit B [Cucumis sativus] >XP_011650247.1 DNA polymerase alpha subunit B [Cucumis sativus] >XP_011650259.1 DNA polymerase alpha subunit B [Cucumis sativus] >XP_031738804.1 DNA polymerase alpha subunit B [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 1.5e-43
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 528 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 587

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINA VVSI
Sbjct: 588 CVNPGRLAKGEGGGTFVELNYFGRPDQINAMVVSI 622

[Close]

BLAST of HG10020626 vs. NCBI nr
Match: KAE8652527.1 (hypothetical protein Csa_013070, partial [Cucumis sativus])

HSP 1 Score: 186.0 bits (471), Expect = 1.5e-43
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 554 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 613

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINA VVSI
Sbjct: 614 CVNPGRLAKGEGGGTFVELNYFGRPDQINAMVVSI 648

[Close]

BLAST of HG10020626 vs. ExPASy Swiss-Prot
Match: Q14181 (DNA polymerase alpha subunit B OS=Homo sapiens OX=9606 GN=POLA2 PE=1 SV=2)

HSP 1 Score: 75.1 bits (183), Expect = 4.9e-13
Identity = 39/85 (45.88%), Postives = 53/85 (62.35%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           S+YPLYPP E + +D+        + V PD+LI+PS++++FVK + LG          C+
Sbjct: 507 SYYPLYPPQEDMAIDYESFYVYAQLPVTPDVLIIPSELRYFVKDV-LG----------CV 566

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRP 89
           CVNPGRL KG+ GGTF  L Y  RP
Sbjct: 567 CVNPGRLTKGQVGGTFARL-YLRRP 579

[Close]

BLAST of HG10020626 vs. ExPASy Swiss-Prot
Match: P33611 (DNA polymerase alpha subunit B OS=Mus musculus OX=10090 GN=Pola2 PE=1 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 4.6e-11
Identity = 34/79 (43.04%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           S+YPLYPP E + +D+        + V PD+ I+PS++++FVK I             C+
Sbjct: 507 SYYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKDI-----------FGCV 566

Query: 64  CVNPGRLAKGEGGGTFVEL 83
           CVNPGRL KG+ GGTF  L
Sbjct: 567 CVNPGRLTKGQVGGTFGRL 574

[Close]

BLAST of HG10020626 vs. ExPASy Swiss-Prot
Match: O89043 (DNA polymerase alpha subunit B OS=Rattus norvegicus OX=10116 GN=Pola2 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.0e-10
Identity = 33/79 (41.77%), Postives = 47/79 (59.49%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           S+YPLYPP E + +D+        + V PD+ I+PS++++FVK I             C+
Sbjct: 507 SYYPLYPPHEDMAIDYENFYTYAQLPVTPDVFIVPSELRYFVKDI-----------FGCV 566

Query: 64  CVNPGRLAKGEGGGTFVEL 83
           C+NPGRL KG+ GGTF  L
Sbjct: 567 CMNPGRLTKGQVGGTFGRL 574

[Close]

BLAST of HG10020626 vs. ExPASy Swiss-Prot
Match: Q9VB62 (DNA polymerase alpha subunit B OS=Drosophila melanogaster OX=7227 GN=PolA2 PE=1 SV=2)

HSP 1 Score: 61.6 bits (148), Expect = 5.6e-09
Identity = 31/74 (41.89%), Postives = 46/74 (62.16%), Query Frame = 0


Query: 4   SFYPLYPPA-EGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARC 63
           SFYPLYPPA E +  D  LA K   +  +P++LILPSD +HF+++++            C
Sbjct: 518 SFYPLYPPADEDMAYDSQLALKYAQLKQLPNVLILPSDQRHFIRLVN-----------DC 577

Query: 64  ICVNPGRLAKGEGG 77
           + +NPGR+A  +GG
Sbjct: 578 LVINPGRVADKKGG 580

[Close]

BLAST of HG10020626 vs. ExPASy Swiss-Prot
Match: Q58D13 (DNA polymerase alpha subunit B OS=Bos taurus OX=9913 GN=POLA2 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.5e-04
Identity = 19/44 (43.18%), Postives = 29/44 (65.91%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKV 48
           S+YPLYPP E + +D+        + V PD+ I PS++++FVKV
Sbjct: 509 SYYPLYPPQEDMAIDYENFYLYAQLPVTPDVFIAPSELRYFVKV 552

[Close]

BLAST of HG10020626 vs. ExPASy TrEMBL
Match: A0A1S3BUF0 (DNA polymerase alpha subunit B OS=Cucumis melo OX=3656 GN=LOC103493371 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.9e-44
Identity = 91/95 (95.79%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 528 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 587

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI
Sbjct: 588 CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 622

[Close]

BLAST of HG10020626 vs. ExPASy TrEMBL
Match: A0A5D3BJQ2 (DNA polymerase alpha subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001520 PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.9e-44
Identity = 91/95 (95.79%), Postives = 93/95 (97.89%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 549 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 608

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI
Sbjct: 609 CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 643

[Close]

BLAST of HG10020626 vs. ExPASy TrEMBL
Match: A0A0A0LQA4 (DNA polymerase alpha subunit B OS=Cucumis sativus OX=3659 GN=Csa_1G039840 PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.3e-44
Identity = 90/95 (94.74%), Postives = 92/95 (96.84%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAP+ALDISVIPDILILPSDMKHFVKVISLGEG S EEQARC+
Sbjct: 645 SFYPLYPPAEGVPLDFSLAPQALDISVIPDILILPSDMKHFVKVISLGEGGSEEEQARCV 704

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVELNYFGRPDQINA VVSI
Sbjct: 705 CVNPGRLAKGEGGGTFVELNYFGRPDQINAMVVSI 739

[Close]

BLAST of HG10020626 vs. ExPASy TrEMBL
Match: A0A6J1FZE8 (DNA polymerase alpha subunit B OS=Cucurbita moschata OX=3662 GN=LOC111449321 PE=3 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.6e-41
Identity = 85/95 (89.47%), Postives = 90/95 (94.74%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEG + EE+A C+
Sbjct: 528 SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEERANCV 587

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVEL YFG PD+INA+VVSI
Sbjct: 588 CVNPGRLAKGEGGGTFVELKYFGTPDKINAAVVSI 622

[Close]

BLAST of HG10020626 vs. ExPASy TrEMBL
Match: A0A6J1HSI8 (DNA polymerase alpha subunit B OS=Cucurbita maxima OX=3661 GN=LOC111466380 PE=3 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 4.4e-41
Identity = 84/95 (88.42%), Postives = 90/95 (94.74%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEG + EE+A C+
Sbjct: 526 SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGRADEERANCV 585

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEGGGTFVEL YFG PD++NA+VVSI
Sbjct: 586 CVNPGRLAKGEGGGTFVELKYFGTPDKLNAAVVSI 620

[Close]

BLAST of HG10020626 vs. TAIR 10
Match: AT1G67630.1 (DNA polymerase alpha 2 )

HSP 1 Score: 137.1 bits (344), Expect = 7.5e-33
Identity = 65/95 (68.42%), Postives = 79/95 (83.16%), Query Frame = 0


Query: 4   SFYPLYPPAEGVPLDFSLAPKALDISVIPDILILPSDMKHFVKVISLGEGSSGEEQARCI 63
           SFYPLYPP E +P D SLAPKAL IS IPDIL+LPSDM++FVK++SLGE    E+ A+C+
Sbjct: 529 SFYPLYPPPESLPYDSSLAPKALQISSIPDILLLPSDMRYFVKILSLGE---EEDAAKCV 588

Query: 64  CVNPGRLAKGEGGGTFVELNYFGRPDQINASVVSI 99
           CVNPGRLAKGEG GTFVEL Y G P+ ++AS++SI
Sbjct: 589 CVNPGRLAKGEGAGTFVELTYKGGPESMHASIISI 620

The following BLAST results are available for this feature:

BLAST of HG10020626 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038886670.1	3.1e-44	96.84	LOW QUALITY PROTEIN: DNA polymerase alpha subunit B [Benincasa hispida]	[more]
TYJ98951.1	4.0e-44	95.79	DNA polymerase alpha subunit B [Cucumis melo var. makuwa]	[more]
XP_008452343.1	4.0e-44	95.79	PREDICTED: DNA polymerase alpha subunit B [Cucumis melo] >XP_008452395.1 PREDICT...	[more]
XP_004137446.1	1.5e-43	94.74	DNA polymerase alpha subunit B [Cucumis sativus] >XP_011650247.1 DNA polymerase ...	[more]
KAE8652527.1	1.5e-43	94.74	hypothetical protein Csa_013070, partial [Cucumis sativus]	[more]

BLAST of HG10020626 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q14181	4.9e-13	45.88	DNA polymerase alpha subunit B OS=Homo sapiens OX=9606 GN=POLA2 PE=1 SV=2	[more]
P33611	4.6e-11	43.04	DNA polymerase alpha subunit B OS=Mus musculus OX=10090 GN=Pola2 PE=1 SV=2	[more]
O89043	1.0e-10	41.77	DNA polymerase alpha subunit B OS=Rattus norvegicus OX=10116 GN=Pola2 PE=1 SV=2	[more]
Q9VB62	5.6e-09	41.89	DNA polymerase alpha subunit B OS=Drosophila melanogaster OX=7227 GN=PolA2 PE=1 ...	[more]
Q58D13	5.5e-04	43.18	DNA polymerase alpha subunit B OS=Bos taurus OX=9913 GN=POLA2 PE=2 SV=1	[more]

BLAST of HG10020626 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BUF0	1.9e-44	95.79	DNA polymerase alpha subunit B OS=Cucumis melo OX=3656 GN=LOC103493371 PE=3 SV=1	[more]
A0A5D3BJQ2	1.9e-44	95.79	DNA polymerase alpha subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A0A0LQA4	7.3e-44	94.74	DNA polymerase alpha subunit B OS=Cucumis sativus OX=3659 GN=Csa_1G039840 PE=3 S...	[more]
A0A6J1FZE8	2.6e-41	89.47	DNA polymerase alpha subunit B OS=Cucurbita moschata OX=3662 GN=LOC111449321 PE=...	[more]
A0A6J1HSI8	4.4e-41	88.42	DNA polymerase alpha subunit B OS=Cucurbita maxima OX=3661 GN=LOC111466380 PE=3 ...	[more]

BLAST of HG10020626 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G67630.1	7.5e-33	68.42	DNA polymerase alpha 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.60.21.60		coord: 1..95 e-value: 8.5E-20 score: 72.6
IPR016722	DNA polymerase alpha, subunit B	PANTHER	PTHR23061	DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT	coord: 3..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10020626.1	HG10020626.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0006260	DNA replication
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding