HG10020622 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020622
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRHOMBOID-like protein 12
LocationChr05: 1009223 .. 1013507 (-)
RNA-Seq ExpressionHG10020622
SyntenyHG10020622
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAAATTTTTTTCTCGCAAACTTGCATCAAGGCTTTTGAATTCCTACACCAACTCTCTATATTGTAAGGATTGCGCTTCTTTTGCCACACTAGTGCGAACCCATAACCAAAACCACCTCTTTGGCTCATTCCTCACTCGCCCACTTCAGGATCACTCGCGTTCATGGAGTTCACATCCAAGTTTTGCGCAGAAAATTCATGGGTTTTTCTCAGTCCCGGCGGCCGGAAAGCGATTCATCTTCATTCCTTCAAATACCCTTTTCAAGGGGGCAATTAAGAGTCTAGTAGAAAGTAGGTCTCTCTTTACGGGAACCCCATTTTCGAAACTAAGATTTCAGTTGAAACCAGGTTTTGATCATCAATGGGGAGGCAGGTAATGTGTTTGCTCTTTTTGCAACTTTGGCTGTTTTATCAGAAAATTAACTTCTTATATGTACGAATGAATTGGTCGCTGAAGAATGTGAAGTCCTTGTTTAAAATCAACTCATTCAGCGCTGAATTTCGTAGTGTCCATGATTTCATTTTTCGATGGTGAATTCGAATTCTCCCAATTGTATTGCCCTGTTGAAGTTTGCAAACGGTGTGCCTCACGTTGCATAACATTATGAACTTCAATTAAATTGACTGATTCCATGAGCAATTGGGAAGTCTAAATTGTTCAAGGACAATTATTTAACTTCGTCAAAATTTTTGGAACACAAACGGTTATTATTTCACATTGGGAGTTAGTGGAGTTTCACTAAATATTGTCTGGGTTTTGTTTAAATATTAAAACACAAACGGTTATTATTTCACATTGGGAGTTCTTGAAATTTTTGGAACCTGATCTTTACAATTGTAGGGATATTATCATGATGCTTTCTGTTTCATAGGAGACTAGCATTGTATTTTAGTCGCCCCCCATGGCATATAGACTCTTACTGCTTGCTGTTATGAAACTTCCTTCTTTGTTATTTTAGGATTAGCATTGTTATTATCATGGGTTGCTTTAAGTTATTATTTAACATTCAAAATAACTTCATAACCTTCTACATTTGAATCGGCTTGTTGAATCTTTTAGTATTGAATACAATGAAACAGGTTATAATCCTTTTAACAAAGGGGAGGATGATTGGTATTTGATAAACAAGAGTTGATTATTTGGGAAAGTCCTTATTTTTGTTTCTCCTATTCACAGGCGATCATGGTTCCAAAGGCTGACGCCTAATGATGTGGTGTTGGGCTTGATCATAGCTAATGTTGCTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTGTACGTCCTCGATTGCAGTTACATGCCTCAGAATTGTAGCACATATAGACACTGTTTCTTCTCACCTCTCATTTTGCTTAAAAGTACATTTCTCTTAAGTGGCCGTTCACGAGGAATCGGGGTTTATGGGAAGCCATTCATGAGGGATTCAGGATTTATGGGAGTCAATAATGTCTAGACATCTAGATATATGAGTATTTGCATGACTAGGAATCAAGAATACTCATGTTGGTTTTGCATGATATGTTCATGACGATTTTGTAATAGTGGCTGCAAATATTAGATAACTACATTGCCAATCCGAGCATGATTCAAAGTGCACCTTGCGCTTTGAAAACATTGCCATATAATTGTAATAAAACATCCCTTTGTTCCACTGATGCCTCTATAATTGGCAACAAGAGGATTTGGATTCCGGATGGGATATATATCTTTAAAACCCATTTCATCTCTTGGATATTCAAATTATCCCTCCATTTGCATCTCAAACACCCACAATGCTTAGTAAACCACATTCATTGAAAGAGAAACATGGAATTTGAGATGTCCGCAACGTGGACATTTGAGACTTCTCCAGATGAATGGCCCTAATAGTTGTGATCAACTTAACATCATTAGTAGTCGTAGTCGTAGTAGTTATTTTTATTATTTCCAAATAGAAAACCATGGTTTGAGAAATATTTGTGGCAAAGATAAGATATTAGATGCTGTTTTGGAGTTTTAAGCTTCAAAGCTTGGAAGTTCAATTCAACTGTTCTGTTGGTTACATTATATTTTCTGTTTTTCAATGTTCTCTATACAGATCTCTATAGAAAATGTTAGAAGCGGCCGCATACATACATTGATTACATCAGCCTTCAGTCACGTAGAAATTGGCCATATAGTATCCAACATGATTGGACTATACTTCTTTGGGATGAACGTATGGCTATCTTTCTTTGAACTTCTATTAGGTCCTGATATTTATTTTATTTTATTTTTTCCTCTGTTTATATGTGTGTGGCTTATGTAATTTTTTTTTTTGTTATGATCTGATCTTTACCACTTTCCTCTTTTTGGCTTATATTCTAATCTTGTAGTTGCGTTTTTCTTTTACACATAATCTTATAGTTATTTATATTATGCTTTTCTCTTTGGTATTATCTCATTGTGAGGTAGCTATGGTAATATATTATATTTTAACAGCCACATTTGACGGTCTATCTTAAATTTTTGGCCTTGTATGCTGAATTTAGTGTTCATACTGGATTTGAAGTAATATATTTTGATTCAAGTTGTTATATGGTGAGCTCTCAAGCCTAATTTTTTTTTTTTCAAAATTTCCATATGTTGTGGATAGTGTGCTTAAATAGTCATAAGTATTTAAGAAAAATTCTATAAGTGCTCACCAAGTTATTGCACACCCCAAGCTCACTATTAGTCTACATGTGAACTATAATCCAATCCAGTCTGTTTGTCTCACGAGTTTGTATGCTACTTATAAAGTTAGCTTTAATGTTTTGCCATGGTCTTTCCAGAATATGATAATACCCCATTAGTGTTAAAACCTTTTCCCCAACCATTTTAGCATTGATATTTGACCTTATATATTTTGCCTTTAACAGATCGGTAGGGTATTTGGTCCTGAGTTTTTGTTGAAATTGTATCTGGCTGGAGCAATTGGTGGGTCAGCATTTTACTTGGCGCATCATCTATTTCAAGCATCGTCCTCAAAGGTATGCAAATTGTGTAAGGGCCCAGTTCTTTCATTCAAATTGTAGGCACGCTTATCTTGCAAATTTTTCGGGTCGCAGAGGTGTAACCGCTTCTGATATAATGTAAAGGCAGCATAGGTGTAACCGCTTCTGATATAATGTAAAGGCAGCATAAAAAAATTGAGTTAGATTTATGTTATGATGATGCAATGCACTAAACTAGAACCTAAGCTTCATTTGGTGTCTTTCATGTAGTTGAGATCACTTTGATACCGATTGTGCATTCACAATGGTAGAAGAGAAGTTCATCTGCCAATGGAACAAACCATCTTGATAGGAATTCTCTTTATACATTCATGAATATAAAATCTGTTTTGTGGGAGTGGGTTTTCTCAATTCCCTGCCCTTAGGCTGTTATGTCGGCTCTTTTGATGAATGTACATATCCGTTATTTGCTGATTGTTGACTGTTTTACAAACTGAATCTTAAACCTTGTTTGGTGGTTTTCTACTTTCTACAGAGCCGGCCATTTTGGGGCAAGGACCCAGTACGAGCACAAGGATTGGTATGCTTTTGGAAATCCTTCTAGTTCTTTACTTTTTTCAACTGTGGAAGCCAACTGAAGACCTCAGTTATCTCCTTAATAAACCTGTTCACTTCCAACGCAAAATTTGTTTTTCTGAAATATCCATTCGTTGTATGCTCTGTGTACATTGTTTCTCCTGTTGTTTGGATATGGGACTTTGTTCCTTTAACCCTACATTATACTGATCACCTTCGACTTTTGAACATCAGCATACTTATTAAAACTTGAATCATCATTGGCGTTCGTTTTGCTTGATCACTTAGGGCGCGAGCGGAGCGGTAAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAGTCTACCCTCTATCTCGAATTCTTTATACCTGTTCCTGCCATCTTACTGGTATGCTCAACTCTAAATAGCACAAAGATTCACAGTTCACTATGAAAAATCTTGAAAGAGTCCTTGTTGTTTCATTGCAGGGCATTTTTCTCATTGGAAAAGACATGTTGAGGATACTAGAGGTATGATGTTTAATTGAATACCTAAACTTCTCCATCCAACTCCACCTTCAGTTCTTCTCTCTGTTATATATAGCTGGCCTCATCCTCTTAGACAAATTCATAAAATCTGTATGTTCTCTGCAGAATGACAGCCAAATTTCAGGATCTGCCCACCTGGGGGGTGCTGCAGTTGCAGCCTTAGCCTGGGCTCGAGTTCGCCGGGGACGTTTCTGA

mRNA sequence

ATGAACAAATTTTTTTCTCGCAAACTTGCATCAAGGCTTTTGAATTCCTACACCAACTCTCTATATTGTAAGGATTGCGCTTCTTTTGCCACACTAGTGCGAACCCATAACCAAAACCACCTCTTTGGCTCATTCCTCACTCGCCCACTTCAGGATCACTCGCGTTCATGGAGTTCACATCCAAGTTTTGCGCAGAAAATTCATGGGTTTTTCTCAGTCCCGGCGGCCGGAAAGCGATTCATCTTCATTCCTTCAAATACCCTTTTCAAGGGGGCAATTAAGAGTCTAGTAGAAAGTAGGTCTCTCTTTACGGGAACCCCATTTTCGAAACTAAGATTTCAGTTGAAACCAGGTTTTGATCATCAATGGGGAGGCAGGCGATCATGGTTCCAAAGGCTGACGCCTAATGATGTGGTGTTGGGCTTGATCATAGCTAATGTTGCTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTATCGGTAGGGTATTTGGTCCTGAGTTTTTGTTGAAATTGTATCTGGCTGGAGCAATTGGTGGGTCAGCATTTTACTTGGCGCATCATCTATTTCAAGCATCGTCCTCAAAGAGCCGGCCATTTTGGGGCAAGGACCCAGTACGAGCACAAGGATTGGGCGCGAGCGGAGCGGTAAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAGTCTACCCTCTATCTCGAATTCTTTATACCTGTTCCTGCCATCTTACTGGGCATTTTTCTCATTGGAAAAGACATGTTGAGGATACTAGAGAATGACAGCCAAATTTCAGGATCTGCCCACCTGGGGGGTGCTGCAGTTGCAGCCTTAGCCTGGGCTCGAGTTCGCCGGGGACGTTTCTGA

Coding sequence (CDS)

ATGAACAAATTTTTTTCTCGCAAACTTGCATCAAGGCTTTTGAATTCCTACACCAACTCTCTATATTGTAAGGATTGCGCTTCTTTTGCCACACTAGTGCGAACCCATAACCAAAACCACCTCTTTGGCTCATTCCTCACTCGCCCACTTCAGGATCACTCGCGTTCATGGAGTTCACATCCAAGTTTTGCGCAGAAAATTCATGGGTTTTTCTCAGTCCCGGCGGCCGGAAAGCGATTCATCTTCATTCCTTCAAATACCCTTTTCAAGGGGGCAATTAAGAGTCTAGTAGAAAGTAGGTCTCTCTTTACGGGAACCCCATTTTCGAAACTAAGATTTCAGTTGAAACCAGGTTTTGATCATCAATGGGGAGGCAGGCGATCATGGTTCCAAAGGCTGACGCCTAATGATGTGGTGTTGGGCTTGATCATAGCTAATGTTGCTGTTTTCCTCCTCTGGCGGATTGCTGATCCTATCTTTATGTCAAAAAACTTTACTATCGGTAGGGTATTTGGTCCTGAGTTTTTGTTGAAATTGTATCTGGCTGGAGCAATTGGTGGGTCAGCATTTTACTTGGCGCATCATCTATTTCAAGCATCGTCCTCAAAGAGCCGGCCATTTTGGGGCAAGGACCCAGTACGAGCACAAGGATTGGGCGCGAGCGGAGCGGTAAATGCTATCATGTTGCTTGATATATTCCTCTTCCCAAAGTCTACCCTCTATCTCGAATTCTTTATACCTGTTCCTGCCATCTTACTGGGCATTTTTCTCATTGGAAAAGACATGTTGAGGATACTAGAGAATGACAGCCAAATTTCAGGATCTGCCCACCTGGGGGGTGCTGCAGTTGCAGCCTTAGCCTGGGCTCGAGTTCGCCGGGGACGTTTCTGA

Protein sequence

MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSHPSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDHQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFTIGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQASSSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQISGSAHLGGAAVAALAWARVRRGRF
Homology
BLAST of HG10020622 vs. NCBI nr
Match: XP_038893963.1 (RHOMBOID-like protein 12, mitochondrial [Benincasa hispida])

HSP 1 Score: 521.2 bits (1341), Expect = 6.0e-144
Identity = 273/336 (81.25%), Postives = 280/336 (83.33%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKLASRL NS+ NS +CKDCASFATLVRTHNQNHLF S LTRPLQD SRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSFANSQHCKDCASFATLVRTHNQNHLFASSLTRPLQDRSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SFA+KIHGF SV   GKRFI  PSNTLFKG+IKSLVES SLFTGTPFSKLRFQLKPGFD
Sbjct: 61  RSFAEKIHGFSSVAVVGKRFILNPSNTLFKGSIKSLVESSSLFTGTPFSKLRFQLKPGFD 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
            QWGGR+SWFQRLTPNDVVLGLIIANVAVFLLW+IADPIFMSKNFT              
Sbjct: 121 RQWGGRQSWFQRLTPNDVVLGLIIANVAVFLLWQIADPIFMSKNFTISIENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IGRVFGPEFLLKLYLAGAIGGSAFYLAH+LFQAS
Sbjct: 181 ITSAFSHKEIGHIVSNMIGLYFFGMNIGRVFGPEFLLKLYLAGAIGGSAFYLAHNLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. NCBI nr
Match: XP_008452017.1 (PREDICTED: RHOMBOID-like protein 12, mitochondrial [Cucumis melo])

HSP 1 Score: 520.0 bits (1338), Expect = 1.3e-143
Identity = 269/336 (80.06%), Postives = 279/336 (83.04%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKLASRL NSY NS+YCKDCASF+TL RTHNQNHLFGSF+TRPLQDHSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SF QKIHGFFSVP  GKR +  PSNT FKG++KS+VESRSLFTGTPFSKL+FQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           HQWGGRRSWFQRLTP DVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IG VFG EFLLKLYLAGAIGGSAFYLAHHLFQAS
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNIGSVFGSEFLLKLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSR FWGKDPVRAQGLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. NCBI nr
Match: TYJ98948.1 (RHOMBOID-like protein 12 [Cucumis melo var. makuwa])

HSP 1 Score: 518.5 bits (1334), Expect = 3.9e-143
Identity = 268/336 (79.76%), Postives = 278/336 (82.74%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKLASRL NSY NS+YCKDCASF+TL RTHNQNHLFGSF+TRPLQDHSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SF QKIHGFFSVP  GKR +  PSNT FKG++KS+VESRSLFTGTPFSKL+FQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           HQWGGRRSWFQRLTP DVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IG VFG EFLLKLYLAGAIGGSAFYL HHLFQAS
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNIGSVFGSEFLLKLYLAGAIGGSAFYLVHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSR FWGKDPVRAQGLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. NCBI nr
Match: XP_004137444.1 (RHOMBOID-like protein 12, mitochondrial [Cucumis sativus] >KGN64067.1 hypothetical protein Csa_013374 [Cucumis sativus])

HSP 1 Score: 517.7 bits (1332), Expect = 6.6e-143
Identity = 267/336 (79.46%), Postives = 280/336 (83.33%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKL SRL NSY NSL CKDCASF+TLVRTHNQNHLFGSF+TRP+QDHSRSWSSH
Sbjct: 1   MNKLFSRKLVSRLSNSYANSLDCKDCASFSTLVRTHNQNHLFGSFVTRPIQDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SFAQKIHGFFSVP  GKRFI  PSNT FKG++KS+VESRSLFTGTPFSKL+FQLKPGF 
Sbjct: 61  RSFAQKIHGFFSVPVVGKRFIVNPSNTYFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           HQWGGRRSWFQRLTPN+VVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HQWGGRRSWFQRLTPNEVVLGLIIANVAVFLLWRIADPIFMSKNFTISLENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IG VFG EFLLKLYLAGAIGGSAFYLAHHLFQA 
Sbjct: 181 ITSAFSHIDTGHIVSNMVGLYFFGMNIGNVFGSEFLLKLYLAGAIGGSAFYLAHHLFQAL 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSR FWG+DPVRA+GLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGEDPVRAKGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. NCBI nr
Match: KAG7019987.1 (RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 503.8 bits (1296), Expect = 9.9e-139
Identity = 263/336 (78.27%), Postives = 276/336 (82.14%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNKFFSRKLASRL NSY NS YC+DCASFAT VRT NQN+ +GSF+TRP++DHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SF +KIHGFFS P  GKRFI  PSNTLFKG+IKSLV  RSLF G+PFSKL+FQLKPGFD
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           H+W GRR+WFQRLT NDVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IGRVFGPEFLL LYLAGAIGGSAFYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SS+SRPFWGKDP RAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. ExPASy Swiss-Prot
Match: Q9FZ81 (RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.1e-60
Identity = 151/339 (44.54%), Postives = 195/339 (57.52%), Query Frame = 0

Query: 6   SRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFL-----TRPLQ--DHSRSWS 65
           S +L   L N  T+S   +   +F +L + +   H    +L     + P+   D S+ W 
Sbjct: 14  SSRLTKLLANPTTHSHLNRQ--TFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWR 73

Query: 66  SHPSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPG 125
           S     +KI GFF+  A G + +           + +LVESR  F G+ F K  F+ +  
Sbjct: 74  S-----EKIRGFFA-SALGNKAV----------KLGNLVESRVGFIGSQFPKKGFEFQRF 133

Query: 126 FDHQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNF------------- 185
              Q  G + W Q L+  DVVLGL+IAN  VF++WR+ +  FM  NF             
Sbjct: 134 SGFQRRGWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLH 193

Query: 186 ---------------------------TIGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQ 245
                                      +I R FGP+FLLKLYLAGA+GGS FYL HH + 
Sbjct: 194 TLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGSVFYLIHHAYM 253

Query: 246 ASSS-KSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 297
           A++S K +  + +DP R  GLGASGAVNAIMLLDIFL P++TLYLEFFIPVPA+LLGIFL
Sbjct: 254 AATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIPVPAMLLGIFL 313

BLAST of HG10020622 vs. ExPASy Swiss-Prot
Match: Q84WG3 (Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 4.6e-30
Identity = 97/281 (34.52%), Postives = 138/281 (49.11%), Query Frame = 0

Query: 62  SFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDH 121
           S + ++HGFF    AGK       NT  K    +++ESR+ F  +      F+       
Sbjct: 52  SRSARVHGFF----AGKL-----GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRI--ADPIFMSKNF-------------- 181
           Q  G +SW      N VV GL+IAN AVF +WR+   D ++M KNF              
Sbjct: 112 QKRGWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIHT 171

Query: 182 --------------------------TIGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQA 241
                                      I R FGP +LLKLY AGA+GGS F+L+ H    
Sbjct: 172 LITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHALSV 231

Query: 242 SSSKSRPFWGKDPVRAQ--GLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 297
            S K +    KD ++     LGA+G V AI LLD+ L+PK T Y    + VP +  GI+ 
Sbjct: 232 ISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAGIYS 291

BLAST of HG10020622 vs. ExPASy Swiss-Prot
Match: B3H707 (Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17 PE=2 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 5.8e-25
Identity = 96/311 (30.87%), Postives = 140/311 (45.02%), Query Frame = 0

Query: 34  RTHNQNHLFGSFLTRPLQDHSRSWSSHPSF-------AQKIHGFFSVPAAGKRFIFIPSN 93
           + +   HL  S L+ P        SS P F       + K+HGFF+             N
Sbjct: 31  KPNQSRHLLPSRLSSP--------SSVPHFVPSAVSRSAKVHGFFASKL---------GN 90

Query: 94  TLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDHQWGGRRSWFQRLTPNDVVLGLIIAN 153
           T  K    +++ESR+ F  +      F+       Q  G +SW      N VV GL+IAN
Sbjct: 91  TNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIAN 150

Query: 154 VAVFLLWRIADPIFMSKNFT-----------------------------------IGRVF 213
            AVF +WR++D  +M   ++                                   I R  
Sbjct: 151 AAVFTMWRVSDRSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTL 210

Query: 214 GPEFLLKLYLAGAIGGSAFYLAHHLFQASSSKS----RPFWGKDPVRAQGLGASGAVNAI 273
           GP +LLKLY AGA+GGS  +L++H   A+        +      P+ +Q LGA G++ AI
Sbjct: 211 GPLYLLKLYFAGALGGSVCFLSYHALLATLKGEGVVIKDHQSTAPI-SQLLGADGSMFAI 270

Query: 274 MLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQ--ISGSAHLGGAAVAA 297
            LLD+F++PK T Y    + V  ++  I  +G ++L I E       S S  LGG  VAA
Sbjct: 271 ALLDMFIYPKVTTYFALMLRV-HVMFRIINLGVEILNIPEGGPNHIASSSGQLGGVVVAA 320

BLAST of HG10020622 vs. ExPASy TrEMBL
Match: A0A1S3BSX2 (RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 PE=3 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 6.5e-144
Identity = 269/336 (80.06%), Postives = 279/336 (83.04%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKLASRL NSY NS+YCKDCASF+TL RTHNQNHLFGSF+TRPLQDHSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SF QKIHGFFSVP  GKR +  PSNT FKG++KS+VESRSLFTGTPFSKL+FQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           HQWGGRRSWFQRLTP DVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IG VFG EFLLKLYLAGAIGGSAFYLAHHLFQAS
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNIGSVFGSEFLLKLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSR FWGKDPVRAQGLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. ExPASy TrEMBL
Match: A0A5D3BLA0 (RHOMBOID-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G001490 PE=3 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 1.9e-143
Identity = 268/336 (79.76%), Postives = 278/336 (82.74%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKLASRL NSY NS+YCKDCASF+TL RTHNQNHLFGSF+TRPLQDHSRSWSSH
Sbjct: 1   MNKLFSRKLASRLSNSYANSIYCKDCASFSTLGRTHNQNHLFGSFVTRPLQDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SF QKIHGFFSVP  GKR +  PSNT FKG++KS+VESRSLFTGTPFSKL+FQLKPGF 
Sbjct: 61  RSFVQKIHGFFSVPVVGKRLMVNPSNTHFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           HQWGGRRSWFQRLTP DVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HQWGGRRSWFQRLTPKDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IG VFG EFLLKLYLAGAIGGSAFYL HHLFQAS
Sbjct: 181 ITSAFSHIDTGHLISNMIGLYFFGMNIGSVFGSEFLLKLYLAGAIGGSAFYLVHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSR FWGKDPVRAQGLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. ExPASy TrEMBL
Match: A0A0A0LTI7 (Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 3.2e-143
Identity = 267/336 (79.46%), Postives = 280/336 (83.33%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKL SRL NSY NSL CKDCASF+TLVRTHNQNHLFGSF+TRP+QDHSRSWSSH
Sbjct: 1   MNKLFSRKLVSRLSNSYANSLDCKDCASFSTLVRTHNQNHLFGSFVTRPIQDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SFAQKIHGFFSVP  GKRFI  PSNT FKG++KS+VESRSLFTGTPFSKL+FQLKPGF 
Sbjct: 61  RSFAQKIHGFFSVPVVGKRFIVNPSNTYFKGSMKSVVESRSLFTGTPFSKLKFQLKPGFV 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           HQWGGRRSWFQRLTPN+VVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HQWGGRRSWFQRLTPNEVVLGLIIANVAVFLLWRIADPIFMSKNFTISLENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IG VFG EFLLKLYLAGAIGGSAFYLAHHLFQA 
Sbjct: 181 ITSAFSHIDTGHIVSNMVGLYFFGMNIGNVFGSEFLLKLYLAGAIGGSAFYLAHHLFQAL 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSR FWG+DPVRA+GLGASGAVNAIMLLDIFLFPK+TLYL+FFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRSFWGEDPVRAKGLGASGAVNAIMLLDIFLFPKATLYLQFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. ExPASy TrEMBL
Match: A0A6J1KPT4 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495401 PE=3 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.1e-138
Identity = 263/336 (78.27%), Postives = 276/336 (82.14%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNKFFSRKLASRL NSY NS YC+DCASFAT VRT NQN+ +GSF+TRP++DHSRSWSSH
Sbjct: 1   MNKFFSRKLASRLSNSYGNSHYCRDCASFATQVRTSNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            S  +KIHGFFS P  GKRFI  PS+TLFKG+IKSLVE RSLF G+PFSKL+FQLKPGFD
Sbjct: 61  RSCTEKIHGFFSGPVVGKRFILNPSSTLFKGSIKSLVE-RSLFMGSPFSKLKFQLKPGFD 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           H+W GRR+WFQRLT NDVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HRWPGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IGRVFGPEFLL LYLAGAIGGSAFYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SSKSRPFWGKDP RAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSKSRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. ExPASy TrEMBL
Match: A0A6J1E6X7 (RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111431274 PE=3 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.4e-138
Identity = 262/336 (77.98%), Postives = 275/336 (81.85%), Query Frame = 0

Query: 1   MNKFFSRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFLTRPLQDHSRSWSSH 60
           MNK FSRKLASRL NSY NS YC+DCASFAT VRT NQN+ +GSF+TRP++DHSRSWSSH
Sbjct: 1   MNKIFSRKLASRLSNSYGNSHYCRDCASFATQVRTGNQNYFYGSFITRPVKDHSRSWSSH 60

Query: 61  PSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFD 120
            SF +KIHGFFS P  GKRFI  PSNTLFKG+IKSLV  RSLF G+PFSKL+FQLKPGFD
Sbjct: 61  RSFTEKIHGFFSGPVVGKRFILNPSNTLFKGSIKSLV-GRSLFMGSPFSKLKFQLKPGFD 120

Query: 121 HQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNFT-------------- 180
           H+W GRR+WFQRLT NDVVLGLIIANVAVFLLWRIADPIFMSKNFT              
Sbjct: 121 HRWSGRRAWFQRLTTNDVVLGLIIANVAVFLLWRIADPIFMSKNFTISVENVRSGRIHTL 180

Query: 181 --------------------------IGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQAS 240
                                     IGRVFGPEFLL LYLAGAIGGSAFYLAHHLFQAS
Sbjct: 181 ITSAFSHVEIGHIVSNMIGLYFFGMNIGRVFGPEFLLGLYLAGAIGGSAFYLAHHLFQAS 240

Query: 241 SSKSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 297
           SS+SRPFWGKDP RAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK
Sbjct: 241 SSESRPFWGKDPARAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGK 300

BLAST of HG10020622 vs. TAIR 10
Match: AT1G18600.1 (RHOMBOID-like protein 12 )

HSP 1 Score: 235.0 bits (598), Expect = 8.0e-62
Identity = 151/339 (44.54%), Postives = 195/339 (57.52%), Query Frame = 0

Query: 6   SRKLASRLLNSYTNSLYCKDCASFATLVRTHNQNHLFGSFL-----TRPLQ--DHSRSWS 65
           S +L   L N  T+S   +   +F +L + +   H    +L     + P+   D S+ W 
Sbjct: 14  SSRLTKLLANPTTHSHLNRQ--TFTSLYKPNQSRHFRTHYLPSSPSSPPVSRFDPSQLWR 73

Query: 66  SHPSFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPG 125
           S     +KI GFF+  A G + +           + +LVESR  F G+ F K  F+ +  
Sbjct: 74  S-----EKIRGFFA-SALGNKAV----------KLGNLVESRVGFIGSQFPKKGFEFQRF 133

Query: 126 FDHQWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRIADPIFMSKNF------------- 185
              Q  G + W Q L+  DVVLGL+IAN  VF++WR+ +  FM  NF             
Sbjct: 134 SGFQRRGWKHWLQGLSDRDVVLGLVIANAGVFVMWRVFNQQFMMNNFMISLDNFKSGRLH 193

Query: 186 ---------------------------TIGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQ 245
                                      +I R FGP+FLLKLYLAGA+GGS FYL HH + 
Sbjct: 194 TLITSAFSHIDIGHIVSNMIGLYFFGTSIARNFGPQFLLKLYLAGALGGSVFYLIHHAYM 253

Query: 246 ASSS-KSRPFWGKDPVRAQGLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 297
           A++S K +  + +DP R  GLGASGAVNAIMLLDIFL P++TLYLEFFIPVPA+LLGIFL
Sbjct: 254 AATSPKGQGAFVRDPSRTPGLGASGAVNAIMLLDIFLHPRATLYLEFFIPVPAMLLGIFL 313

BLAST of HG10020622 vs. TAIR 10
Match: AT1G74130.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 133.3 bits (334), Expect = 3.3e-31
Identity = 97/281 (34.52%), Postives = 138/281 (49.11%), Query Frame = 0

Query: 62  SFAQKIHGFFSVPAAGKRFIFIPSNTLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDH 121
           S + ++HGFF    AGK       NT  K    +++ESR+ F  +      F+       
Sbjct: 52  SRSARVHGFF----AGKL-----GNTNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGF 111

Query: 122 QWGGRRSWFQRLTPNDVVLGLIIANVAVFLLWRI--ADPIFMSKNF-------------- 181
           Q  G +SW      N VV GL+IAN AVF +WR+   D ++M KNF              
Sbjct: 112 QKRGWKSWIN--GANGVVFGLVIANAAVFTMWRVLGKDNMWMVKNFMLSRYSFMTGRIHT 171

Query: 182 --------------------------TIGRVFGPEFLLKLYLAGAIGGSAFYLAHHLFQA 241
                                      I R FGP +LLKLY AGA+GGS F+L+ H    
Sbjct: 172 LITSGFSHVGATHIILNMMGLCYFGARIARSFGPRYLLKLYFAGALGGSVFFLSSHALSV 231

Query: 242 SSSKSRPFWGKDPVRAQ--GLGASGAVNAIMLLDIFLFPKSTLYLEFFIPVPAILLGIFL 297
            S K +    KD ++     LGA+G V AI LLD+ L+PK T Y    + VP +  GI+ 
Sbjct: 232 ISLKGQRVVPKDQLKVPIGKLGANGPVYAITLLDMLLYPKVTTYFGLMLRVP-VFAGIYS 291

BLAST of HG10020622 vs. TAIR 10
Match: AT1G74140.5 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 116.3 bits (290), Expect = 4.1e-26
Identity = 96/311 (30.87%), Postives = 140/311 (45.02%), Query Frame = 0

Query: 34  RTHNQNHLFGSFLTRPLQDHSRSWSSHPSF-------AQKIHGFFSVPAAGKRFIFIPSN 93
           + +   HL  S L+ P        SS P F       + K+HGFF+             N
Sbjct: 31  KPNQSRHLLPSRLSSP--------SSVPHFVPSAVSRSAKVHGFFASKL---------GN 90

Query: 94  TLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDHQWGGRRSWFQRLTPNDVVLGLIIAN 153
           T  K    +++ESR+ F  +      F+       Q  G +SW      N VV GL+IAN
Sbjct: 91  TNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIAN 150

Query: 154 VAVFLLWRIADPIFMSKNFT-----------------------------------IGRVF 213
            AVF +WR++D  +M   ++                                   I R  
Sbjct: 151 AAVFTMWRVSDRSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTL 210

Query: 214 GPEFLLKLYLAGAIGGSAFYLAHHLFQASSSKS----RPFWGKDPVRAQGLGASGAVNAI 273
           GP +LLKLY AGA+GGS  +L++H   A+        +      P+ +Q LGA G++ AI
Sbjct: 211 GPLYLLKLYFAGALGGSVCFLSYHALLATLKGEGVVIKDHQSTAPI-SQLLGADGSMFAI 270

Query: 274 MLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQ--ISGSAHLGGAAVAA 297
            LLD+F++PK T Y    + V  ++  I  +G ++L I E       S S  LGG  VAA
Sbjct: 271 ALLDMFIYPKVTTYFALMLRV-HVMFRIINLGVEILNIPEGGPNHIASSSGQLGGVVVAA 320

BLAST of HG10020622 vs. TAIR 10
Match: AT1G74140.1 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 115.2 bits (287), Expect = 9.2e-26
Identity = 96/311 (30.87%), Postives = 139/311 (44.69%), Query Frame = 0

Query: 34  RTHNQNHLFGSFLTRPLQDHSRSWSSHPSF-------AQKIHGFFSVPAAGKRFIFIPSN 93
           + +   HL  S L+ P        SS P F       + K+HGFF+             N
Sbjct: 31  KPNQSRHLLPSRLSSP--------SSVPHFVPSAVSRSAKVHGFFASKL---------GN 90

Query: 94  TLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDHQWGGRRSWFQRLTPNDVVLGLIIAN 153
           T  K    +++ESR+ F  +      F+       Q  G +SW      N VV GL+IAN
Sbjct: 91  TNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIAN 150

Query: 154 VAVFLLWRIADPIFMSKNFT-----------------------------------IGRVF 213
            AVF +WR++D  +M   ++                                   I R  
Sbjct: 151 AAVFTMWRVSDRSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTL 210

Query: 214 GPEFLLKLYLAGAIGGSAFYLAHHLFQASSSKS----RPFWGKDPVRAQGLGASGAVNAI 273
           GP +LLKLY AGA+GGS  +L++H   A+        +      P+ +Q LGA G++ AI
Sbjct: 211 GPLYLLKLYFAGALGGSVCFLSYHALLATLKGEGVVIKDHQSTAPI-SQLLGADGSMFAI 270

Query: 274 MLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQ--ISGSAHLGGAAVAA 297
            LLD+F++PK T Y    + V  I+     +G ++L I E       S S  LGG  VAA
Sbjct: 271 ALLDMFIYPKVTTYFALMLRVHRII----NLGVEILNIPEGGPNHIASSSGQLGGVVVAA 317

BLAST of HG10020622 vs. TAIR 10
Match: AT1G74140.4 (Rhomboid-related intramembrane serine protease family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.9e-23
Identity = 93/309 (30.10%), Postives = 133/309 (43.04%), Query Frame = 0

Query: 34  RTHNQNHLFGSFLTRPLQDHSRSWSSHPSF-------AQKIHGFFSVPAAGKRFIFIPSN 93
           + +   HL  S L+ P        SS P F       + K+HGFF+             N
Sbjct: 31  KPNQSRHLLPSRLSSP--------SSVPHFVPSAVSRSAKVHGFFASKL---------GN 90

Query: 94  TLFKGAIKSLVESRSLFTGTPFSKLRFQLKPGFDHQWGGRRSWFQRLTPNDVVLGLIIAN 153
           T  K    +++ESR+ F  +      F+       Q  G +SW      N VV GL+IAN
Sbjct: 91  TNLKLKFGNVMESRAGFFSSELPSHGFESGGFTGFQKRGWKSWIN--GANGVVFGLVIAN 150

Query: 154 VAVFLLWRIADPIFMSKNFT-----------------------------------IGRVF 213
            AVF +WR++D  +M   ++                                   I R  
Sbjct: 151 AAVFTMWRVSDRSWMLSTYSFTSGYIHTLITSGFSHIGTSQIILNMIGISYFGSRIARTL 210

Query: 214 GPEFLLKLYLAGAIGGSAFYLAHHLFQASSSKS----RPFWGKDPVRAQGLGASGAVNAI 273
           GP +LLKLY AGA+GGS  +L++H   A+        +      P+ +Q LGA G++ AI
Sbjct: 211 GPLYLLKLYFAGALGGSVCFLSYHALLATLKGEGVVIKDHQSTAPI-SQLLGADGSMFAI 270

Query: 274 MLLDIFLFPKSTLYLEFFIPVPAILLGIFLIGKDMLRILENDSQISGSAHLGGAAVAALA 297
            LLD+F++PK T Y    + V  +  G   I              S S  LGG  VAA+A
Sbjct: 271 ALLDMFIYPKVTTYFALMLRVHVMFGGPNHIA-------------SSSGQLGGVVVAAMA 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893963.16.0e-14481.25RHOMBOID-like protein 12, mitochondrial [Benincasa hispida][more]
XP_008452017.11.3e-14380.06PREDICTED: RHOMBOID-like protein 12, mitochondrial [Cucumis melo][more]
TYJ98948.13.9e-14379.76RHOMBOID-like protein 12 [Cucumis melo var. makuwa][more]
XP_004137444.16.6e-14379.46RHOMBOID-like protein 12, mitochondrial [Cucumis sativus] >KGN64067.1 hypothetic... [more]
KAG7019987.19.9e-13978.27RHOMBOID-like protein 12, mitochondrial [Cucurbita argyrosperma subsp. argyrospe... [more]
Match NameE-valueIdentityDescription
Q9FZ811.1e-6044.54RHOMBOID-like protein 12, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=RBL12... [more]
Q84WG34.6e-3034.52Rhomboid-like protein 16, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL16... [more]
B3H7075.8e-2530.87Rhomboid-like protein 17, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RBL17... [more]
Match NameE-valueIdentityDescription
A0A1S3BSX26.5e-14480.06RHOMBOID-like protein 12, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103493123 ... [more]
A0A5D3BLA01.9e-14379.76RHOMBOID-like protein 12 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A0A0LTI73.2e-14379.46Rhomboid domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G039320 PE... [more]
A0A6J1KPT41.1e-13878.27RHOMBOID-like protein 12, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111495... [more]
A0A6J1E6X71.4e-13877.98RHOMBOID-like protein 12, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT1G18600.18.0e-6244.54RHOMBOID-like protein 12 [more]
AT1G74130.13.3e-3134.52Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.54.1e-2630.87Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.19.2e-2630.87Rhomboid-related intramembrane serine protease family protein [more]
AT1G74140.41.9e-2330.10Rhomboid-related intramembrane serine protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022764Peptidase S54, rhomboid domainPFAMPF01694Rhomboidcoord: 166..291
e-value: 7.5E-14
score: 52.0
IPR035952Rhomboid-like superfamilyGENE3D1.20.1540.10coord: 160..294
e-value: 1.5E-11
score: 46.2
IPR035952Rhomboid-like superfamilySUPERFAMILY144091Rhomboid-likecoord: 135..292
NoneNo IPR availablePANTHERPTHR43731:SF22RHOMBOID-LIKE PROTEIN 12, MITOCHONDRIALcoord: 160..293
coord: 29..161
NoneNo IPR availablePANTHERPTHR43731RHOMBOID PROTEASEcoord: 160..293
coord: 29..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020622.1HG10020622.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004252 serine-type endopeptidase activity