HG10020495 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020495
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionL-ascorbate oxidase-like protein
LocationChr05: 2057 .. 3916 (+)
RNA-Seq ExpressionHG10020495
SyntenyHG10020495
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTAGGTTGACGTTTACCTTGTTGCTATGCCTCCTCTCAGTTGCAGCAATGTCAACTGTTCGGGGTGAAGATCCTTATTTCTTCTTCACGTGGAATGTCACTTATGGCACCATCTCTCCTTTGGGTGTTCCCCAACAAGGCATTCTCATCAACGGTCAATTTCCCGGACCTAATATTAACTCCACGTCCAACAATAACCTAGTCATCAATGTCTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGTATAGTAATTATATTTATATTTACATCTACATGCACATAATTATTAATACATTTACTCTTCATTAATGGATTAATAAAAAGAAACTGAAATTGGGATTGCAGGAGCGGTGTTCAACATAGAAAGAACTCGTGGCAGGATGGGCTACTTGGAACCAATTGCCCAATCCCACCAGGGACAAACTTTACCTATCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAGAGCTGGAGGAGCTTTTGGCGGCCTTCGAATAAATAGTCGCTTACTCATTCCTGTTCCTTATGCTGATCCTGAAGATGATTACACAGTGCTTATTGGTGACTGGTACACCAAGAGTCATTCCACTCTTAAGAAGTTCTTGGACAGTGGTCGCTCCATTGCTAGACCCGATGGGGTCCTAATTAACGGAAAGGTTGCAAAGGGTGATGGAACTGATGAGCCACTCTTCACCATGAAGCCTGGGAAGACTTACAAGTATCGAATTTGCAACGTTGGACTAAAAACATCTCTTAACTTTAGGATCCAAGGCCACACCATGAAGTTGGTAGAGATGGAAGGCTCTCATACTGTCCAAAATGTTTACCAATCACTTGATGTCCATGTGGGACAATGTTTTTCAGTGTTAGTCACAGCCGACCAAGACCCTAAAGACTATTATATGGTTGCATCTACTCGATTCACTAAGAACATTCTTGTTGGTAAAGGCATTGTTCGATACACCAATGGAAAGGGCCCTGTCTCTCATGAAATTCCTGAGGCACCGATGGGTTGGACTTGGTCCCTAAATCAATTTCGTACCTTTCGTTGGAACCTTACTGCCAATGCTGCTAGGCCTAATCCTCAAGGCTCGTATCACTATGGTTCCATTAACATTACTCGCACCATCAAGATAGTCAATTCCGCCACTAAGGTTGATGGTAAGCTGAGATATGCCATCAATGGAGTCTCTCATGTTGACCCTGAAACTCCATTGAAGCTTGCAGAATTCTTCGAAGTCGCGGATAAGGTCTTCAAGTATGATACCATTTCTGACGAAGGATTGGCAAAAGATGGAAACACTGTGACTATTGCTCCTAATGTCGTTAATGCAACCTTTCGCAACTTCGTAGAGATTATATTTGAGAACCACGAGAAGAGCCTCCAGTCATGGCATTTGGATGGCTACTCCTTCTTTGCTGTTGCGTAAGTATCAATGACCATAAATAATTAAAACATATAAATGCATATGTATATTTTCTACATGCGGCTAATGAATTCTTTATCGACGTTTCACACAGCATTGAGCCGGGGAGATGGACTCCAGAGAAACGAAAGAATTACAATCTTCTAGACGCCGTGAGCAGGCATACAATCCAAGTATTTCCTAAATCGTGGGCAGCTATTCTTCTTACTTTAGACAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGACAGAAAATCGTTACTTGGGACAACAACTGTACTTTAGTGTTCTTTCACCCGCTCGCTCCCTCAGAGATGAATACAACATTCCAGATAACACTTTGCTTTGCGGTTTGGTTAAAGACATGCCATTGCCCAAGCCATATACAATCTGA

mRNA sequence

ATGGCTAGGTTGACGTTTACCTTGTTGCTATGCCTCCTCTCAGTTGCAGCAATGTCAACTGTTCGGGGTGAAGATCCTTATTTCTTCTTCACGTGGAATGTCACTTATGGCACCATCTCTCCTTTGGGTGTTCCCCAACAAGGCATTCTCATCAACGGTCAATTTCCCGGACCTAATATTAACTCCACGTCCAACAATAACCTAGTCATCAATGTCTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGAGCGGTGTTCAACATAGAAAGAACTCGTGGCAGGATGGGCTACTTGGAACCAATTGCCCAATCCCACCAGGGACAAACTTTACCTATCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAGAGCTGGAGGAGCTTTTGGCGGCCTTCGAATAAATAGTCGCTTACTCATTCCTGTTCCTTATGCTGATCCTGAAGATGATTACACAGTGCTTATTGGTGACTGGTACACCAAGAGTCATTCCACTCTTAAGAAGTTCTTGGACAGTGGTCGCTCCATTGCTAGACCCGATGGGGTCCTAATTAACGGAAAGGTTGCAAAGGGTGATGGAACTGATGAGCCACTCTTCACCATGAAGCCTGGGAAGACTTACAAGTATCGAATTTGCAACGTTGGACTAAAAACATCTCTTAACTTTAGGATCCAAGGCCACACCATGAAGTTGGTAGAGATGGAAGGCTCTCATACTGTCCAAAATGTTTACCAATCACTTGATGTCCATGTGGGACAATGTTTTTCAGTGTTAGTCACAGCCGACCAAGACCCTAAAGACTATTATATGGTTGCATCTACTCGATTCACTAAGAACATTCTTGTTGGTAAAGGCATTGTTCGATACACCAATGGAAAGGGCCCTGTCTCTCATGAAATTCCTGAGGCACCGATGGGTTGGACTTGGTCCCTAAATCAATTTCGTACCTTTCGTTGGAACCTTACTGCCAATGCTGCTAGGCCTAATCCTCAAGGCTCGTATCACTATGGTTCCATTAACATTACTCGCACCATCAAGATAGTCAATTCCGCCACTAAGGTTGATGGTAAGCTGAGATATGCCATCAATGGAGTCTCTCATGTTGACCCTGAAACTCCATTGAAGCTTGCAGAATTCTTCGAAGTCGCGGATAAGGTCTTCAAGTATGATACCATTTCTGACGAAGGATTGGCAAAAGATGGAAACACTGTGACTATTGCTCCTAATGTCGTTAATGCAACCTTTCGCAACTTCGTAGAGATTATATTTGAGAACCACGAGAAGAGCCTCCAGTCATGGCATTTGGATGGCTACTCCTTCTTTGCTGTTGCCATTGAGCCGGGGAGATGGACTCCAGAGAAACGAAAGAATTACAATCTTCTAGACGCCGTGAGCAGGCATACAATCCAAGTATTTCCTAAATCGTGGGCAGCTATTCTTCTTACTTTAGACAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGACAGAAAATCGTTACTTGGGACAACAACTGTACTTTAGTGTTCTTTCACCCGCTCGCTCCCTCAGAGATGAATACAACATTCCAGATAACACTTTGCTTTGCGGTTTGGTTAAAGACATGCCATTGCCCAAGCCATATACAATCTGA

Coding sequence (CDS)

ATGGCTAGGTTGACGTTTACCTTGTTGCTATGCCTCCTCTCAGTTGCAGCAATGTCAACTGTTCGGGGTGAAGATCCTTATTTCTTCTTCACGTGGAATGTCACTTATGGCACCATCTCTCCTTTGGGTGTTCCCCAACAAGGCATTCTCATCAACGGTCAATTTCCCGGACCTAATATTAACTCCACGTCCAACAATAACCTAGTCATCAATGTCTTCAATAATCTCGATGAACCCTTCCTTCTACATTGGAGCGGTGTTCAACATAGAAAGAACTCGTGGCAGGATGGGCTACTTGGAACCAATTGCCCAATCCCACCAGGGACAAACTTTACCTATCATTTCCAAGTTAAGGACCAAATTGGAAGCTTCTTCTACTACCCATCAACTGCCATGCACAGAGCTGGAGGAGCTTTTGGCGGCCTTCGAATAAATAGTCGCTTACTCATTCCTGTTCCTTATGCTGATCCTGAAGATGATTACACAGTGCTTATTGGTGACTGGTACACCAAGAGTCATTCCACTCTTAAGAAGTTCTTGGACAGTGGTCGCTCCATTGCTAGACCCGATGGGGTCCTAATTAACGGAAAGGTTGCAAAGGGTGATGGAACTGATGAGCCACTCTTCACCATGAAGCCTGGGAAGACTTACAAGTATCGAATTTGCAACGTTGGACTAAAAACATCTCTTAACTTTAGGATCCAAGGCCACACCATGAAGTTGGTAGAGATGGAAGGCTCTCATACTGTCCAAAATGTTTACCAATCACTTGATGTCCATGTGGGACAATGTTTTTCAGTGTTAGTCACAGCCGACCAAGACCCTAAAGACTATTATATGGTTGCATCTACTCGATTCACTAAGAACATTCTTGTTGGTAAAGGCATTGTTCGATACACCAATGGAAAGGGCCCTGTCTCTCATGAAATTCCTGAGGCACCGATGGGTTGGACTTGGTCCCTAAATCAATTTCGTACCTTTCGTTGGAACCTTACTGCCAATGCTGCTAGGCCTAATCCTCAAGGCTCGTATCACTATGGTTCCATTAACATTACTCGCACCATCAAGATAGTCAATTCCGCCACTAAGGTTGATGGTAAGCTGAGATATGCCATCAATGGAGTCTCTCATGTTGACCCTGAAACTCCATTGAAGCTTGCAGAATTCTTCGAAGTCGCGGATAAGGTCTTCAAGTATGATACCATTTCTGACGAAGGATTGGCAAAAGATGGAAACACTGTGACTATTGCTCCTAATGTCGTTAATGCAACCTTTCGCAACTTCGTAGAGATTATATTTGAGAACCACGAGAAGAGCCTCCAGTCATGGCATTTGGATGGCTACTCCTTCTTTGCTGTTGCCATTGAGCCGGGGAGATGGACTCCAGAGAAACGAAAGAATTACAATCTTCTAGACGCCGTGAGCAGGCATACAATCCAAGTATTTCCTAAATCGTGGGCAGCTATTCTTCTTACTTTAGACAATGCTGGAATGTGGAACTTGAGGTCTGAGTTGACAGAAAATCGTTACTTGGGACAACAACTGTACTTTAGTGTTCTTTCACCCGCTCGCTCCCTCAGAGATGAATACAACATTCCAGATAACACTTTGCTTTGCGGTTTGGTTAAAGACATGCCATTGCCCAAGCCATATACAATCTGA

Protein sequence

MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMPLPKPYTI
Homology
BLAST of HG10020495 vs. NCBI nr
Match: KAA0036016.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 525/553 (94.94%), Postives = 536/553 (96.93%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLTFTLLLCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVLIGDWYTKS+STLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSYSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK+AKGDGTDEPLFTM PGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDP+DYYMVAS+RFTKNILVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK P S EIPEAP GW WSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           AT VDGKLRYAINGVSH+DPETPLKLAE+FEVADKVFKYDTISDEG AK  NTVTIAPNV
Sbjct: 361 ATNVDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQLYFSV SPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of HG10020495 vs. NCBI nr
Match: XP_008438536.1 (PREDICTED: L-ascorbate oxidase homolog [Cucumis melo] >TYJ98816.1 L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 524/553 (94.76%), Postives = 535/553 (96.75%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLTFTLLLCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK+AKGDGTDEPLFTM PGKTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDP+DYYMVAS+RFTKNILVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK P S EIPEAP GW WSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           AT +DGKLRYAINGVSH+DPETPLKLAE+FEVADKVFKYDTISDEG AK  NTVTIAPNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQLYFSV SPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of HG10020495 vs. NCBI nr
Match: XP_004137383.1 (L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652494.1 hypothetical protein Csa_013569 [Cucumis sativus])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 518/553 (93.67%), Postives = 537/553 (97.11%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLT +L+LCLLSVAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTLSLMLCLLSVAAMQTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMH+A GAFGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHKAVGAFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLING++AKGDGTDEPLFTMKP KTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGRIAKGDGTDEPLFTMKPEKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTAD+DPKDYYMVASTRFTKNILVGKGI+RYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADKDPKDYYMVASTRFTKNILVGKGIIRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK P S EIPEAP GW WSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           AT VDGKLRYAINGVSHVDPETPLKLAE+FEVADKVFKYDTISDEGL+K  N VT+APNV
Sbjct: 361 ATNVDGKLRYAINGVSHVDPETPLKLAEYFEVADKVFKYDTISDEGLSKTANIVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           +NATFRNFVEI+FEN EKSLQSWHLDGYSFFAVA+EPGRW+PEKRKNYNLLDAVSRHTIQ
Sbjct: 421 LNATFRNFVEIVFENQEKSLQSWHLDGYSFFAVAVEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQ+YFSV SPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQMYFSVQSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDMPLP PYTI
Sbjct: 541 LVKDMPLPPPYTI 553

BLAST of HG10020495 vs. NCBI nr
Match: KAA0040126.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 505/553 (91.32%), Postives = 529/553 (95.66%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARL FT LLCL + A M+TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTSNNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPI PGTNFTYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFR QGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQN YQSLDVHVGQCFSVLVTA+Q+ KDYYMVASTRF KN+LVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           +GKGP S EIPEAP+GW WSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           A+KVDGKLRYAINGVSHVDPETPLKLAE+F +ADKVFKYDTISDEGLA+   TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of HG10020495 vs. NCBI nr
Match: TYK21747.1 (L-ascorbate oxidase-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 505/553 (91.32%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARL FT LLCL + A M+TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSGVQHRKN WQDGLLGTNCPI PGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGVQHRKNCWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFR QGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQN YQSLDVHVGQCFSVLVTA+Q+ KDYYMVASTRF KN+LVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           +GKGP S EIPEAPMGW WSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           A+KVDGKLRYAINGVSHVDPETPLKLAE+F +ADKVFKYDTISDEGLA+   TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of HG10020495 vs. ExPASy Swiss-Prot
Match: Q00624 (L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 8.3e-259
Identity = 406/546 (74.36%), Postives = 465/546 (85.16%), Query Frame = 0

Query: 8   LLLCL-LSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNN 67
           L  CL L+ AA   V  EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINSTSNN
Sbjct: 7   LAACLYLAAAATVVVHAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNN 66

Query: 68  NLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFY 127
           N++INVFNNLDEPFLL W+G+QHRKN WQDG  GT CPI PGTN+TYHFQ KDQIGS+FY
Sbjct: 67  NVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFY 126

Query: 128 YPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSI 187
           YP+T MHRA G +GGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+ LKKFLD GR+I
Sbjct: 127 YPTTGMHRAAGGYGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDGGRTI 186

Query: 188 ARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 247
            RPDG++INGK  KGDG+D PLFT+KPGKTY+ RICNVG+KTS+NFRIQ H MKLVEMEG
Sbjct: 187 GRPDGIVINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGVKTSINFRIQNHKMKLVEMEG 246

Query: 248 SHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNGKGPV 307
           SH +QN Y SLDVHVGQCF  +VTA+Q+PKDYYMVAS+RF K ++   G++RY  GKGP 
Sbjct: 247 SHVLQNDYDSLDVHVGQCFGTIVTANQEPKDYYMVASSRFLKTVITTTGLLRYEGGKGPA 306

Query: 308 SHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSATKVDG 367
           S ++P  P+GW WSLNQFR+FRWNLTA+AARPNPQGSYHYG INITRTIK+VN+  KVDG
Sbjct: 307 SSQLPAGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDG 366

Query: 368 KLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVNATFR 427
           KLR+A+NGVSH +PETPLKLAE+F ++DKVFKYDTI+D+   +    + I PNV+N T R
Sbjct: 367 KLRFALNGVSHTEPETPLKLAEYFGISDKVFKYDTITDDPTPEQIKNIKIEPNVLNITHR 426

Query: 428 NFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSW 487
            FVE++FENHEKS+QSWHLDGYSFF+VA+EPG WTPEKRKNYNLLDAVSRHT+QV+PK W
Sbjct: 427 TFVEVVFENHEKSVQSWHLDGYSFFSVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 486

Query: 488 AAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMP 547
           AAILLT DN GMWN+RSE TE RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK+ P
Sbjct: 487 AAILLTFDNCGMWNVRSENTERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKNTP 546

Query: 548 LP-KPY 552
            P  PY
Sbjct: 547 KPVNPY 552

BLAST of HG10020495 vs. ExPASy Swiss-Prot
Match: P29162 (L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 826.6 bits (2134), Expect = 1.6e-238
Identity = 379/553 (68.54%), Postives = 450/553 (81.37%), Query Frame = 0

Query: 3   RLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 62
           ++TF  LL  LSV  ++    EDPY +F WNVTYGTI+PLGVPQQGILINGQFPGP IN 
Sbjct: 5   KVTFVALLLCLSVGVIA----EDPYLYFNWNVTYGTIAPLGVPQQGILINGQFPGPRINC 64

Query: 63  TSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 122
           TSNNN+V+NVFNNLDEPFL  W+GVQHRKNSWQDG  GT CPI PG NFTY FQVKDQIG
Sbjct: 65  TSNNNIVVNVFNNLDEPFLFTWNGVQHRKNSWQDGTPGTMCPIMPGQNFTYRFQVKDQIG 124

Query: 123 SFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDS 182
           S+ Y+P+TA+HRA G +G L ++SR LIPVP+ +P D+Y V +GDWY K H TLKK LD 
Sbjct: 125 SYSYFPTTALHRAAGGYGALNVHSRALIPVPFDNPADEYNVFVGDWYNKGHKTLKKILDG 184

Query: 183 GRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLV 242
           GR+I RPDG++INGK AK     EPLFTM+ GKTY+YR CN+G+++S+N R QGH MKLV
Sbjct: 185 GRTIGRPDGIIINGKSAKVGEAKEPLFTMEAGKTYRYRFCNLGMRSSVNIRFQGHPMKLV 244

Query: 243 EMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNG 302
           E+EGSHTVQN+Y SLD+HVGQC SVLVTADQ+PKDYY+V S+RF K  L    I+RY NG
Sbjct: 245 ELEGSHTVQNIYDSLDLHVGQCLSVLVTADQEPKDYYLVVSSRFLKQALSSVAIIRYANG 304

Query: 303 KGPVSHEIPEAP----MGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIV 362
           KGP S E+P  P     G  WS+NQFR+FRWNLTA+AARPNPQGSYHYG INITRTIKI 
Sbjct: 305 KGPASPELPTPPPENTEGIAWSMNQFRSFRWNLTASAARPNPQGSYHYGQINITRTIKIF 364

Query: 363 NSATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAP 422
           NS ++V GKLRY +NG+SH + ETPLKL E+F   +K FKYD ++DE  A D + +TIA 
Sbjct: 365 NSMSQVGGKLRYGLNGISHTNGETPLKLVEYFGATNKAFKYDLMADEAPA-DPSKLTIAT 424

Query: 423 NVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHT 482
           NV NAT+RNFVEIIFENHEK+++++HLDGYSFFAVA+EPGRW+PEKRKNYNL+D +SR+ 
Sbjct: 425 NVKNATYRNFVEIIFENHEKTIRTYHLDGYSFFAVAVEPGRWSPEKRKNYNLVDGLSRNN 484

Query: 483 IQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLL 542
           IQV+P SWAAI+LT DNAGMWNLRSE+ E  YLG+QLYFSVLSP+RSLRDEYNIPDN  L
Sbjct: 485 IQVYPNSWAAIMLTFDNAGMWNLRSEMWEKTYLGEQLYFSVLSPSRSLRDEYNIPDNHPL 544

Query: 543 CGLVKDMPLPKPY 552
           CG+VK + +P PY
Sbjct: 545 CGIVKGLSMPAPY 552

BLAST of HG10020495 vs. ExPASy Swiss-Prot
Match: Q9SU40 (Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=1 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 2.6e-159
Identity = 273/559 (48.84%), Postives = 368/559 (65.83%), Query Frame = 0

Query: 9   LLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNL 68
           +L L+    +S     DPY F+ + V+Y T SPLGVPQQ I ING+FPGP IN T+N NL
Sbjct: 6   ILLLVFFVNISFCFAADPYSFYNFEVSYITASPLGVPQQVIAINGKFPGPTINVTTNENL 65

Query: 69  VINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYP 128
           V+NV N LDE  LLHW+G+Q R+ SWQDG+LGTNCPIPP  N+TY FQVKDQIGSFFY+P
Sbjct: 66  VVNVRNKLDEGLLLHWNGIQQRRVSWQDGVLGTNCPIPPKWNWTYEFQVKDQIGSFFYFP 125

Query: 129 STAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIAR 188
           S    RA G FG   +N R +IPVP++ P+ D TV IGDWY ++H+ L+K LD G+ +  
Sbjct: 126 SLHFQRASGGFGSFVVNPRAIIPVPFSTPDGDITVTIGDWYIRNHTALRKALDDGKDLGM 185

Query: 189 PDGVLINGK-------VAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKL 248
           PDGVLINGK           DG D    T+ PGKTY+ R+ NVG+ TSLNFRIQGH + L
Sbjct: 186 PDGVLINGKGPYRYNDTLVADGIDFETITVHPGKTYRLRVSNVGISTSLNFRIQGHNLVL 245

Query: 249 VEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQD-PKDYYMVASTRFTKNIL----VGKGI 308
            E EGS+TVQ  Y SLD+HVGQ +S LVT DQ+   DYY+VAS R     +     G GI
Sbjct: 246 AESEGSYTVQQNYTSLDIHVGQSYSFLVTMDQNASSDYYIVASARVVNETIWRRVTGVGI 305

Query: 309 VRYTNGKGPVSHEIPEAP---MGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITR 368
           ++YTN KG    ++P  P      T+S+NQ R+ RWN++A+ ARPNPQGS+ YGSIN+T 
Sbjct: 306 LKYTNSKGKAKGQLPPGPQDEFDKTFSMNQARSIRWNVSASGARPNPQGSFKYGSINVTD 365

Query: 369 TIKIVN-SATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISD--EGLAKD 428
              + N     + GK R  +NG+S  +P TP++LA+  +V D V+K D       G AK 
Sbjct: 366 VYVLRNMPPVTISGKRRTTLNGISFKNPSTPIRLADKLKVKD-VYKLDFPKRPLTGPAK- 425

Query: 429 GNTVTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNL 488
                +A +++N T+R F+E++ +N++  +QS+H+ GY+FF V ++ G WT   R  YN 
Sbjct: 426 -----VATSIINGTYRGFMEVVLQNNDTKMQSYHMSGYAFFVVGMDYGEWTENSRGTYNK 485

Query: 489 LDAVSRHTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEY 548
            D ++R TIQV+P +W+AIL++LDN G WNLR+E  ++ YLGQ+ Y  V++P  + + E+
Sbjct: 486 WDGIARSTIQVYPGAWSAILISLDNPGAWNLRTENLDSWYLGQETYVRVVNPDENNKTEF 545

Query: 549 NIPDNTLLCGLVKDMPLPK 550
             PDN L CG +  +  P+
Sbjct: 546 GHPDNVLYCGALSKLQKPQ 557

BLAST of HG10020495 vs. ExPASy Swiss-Prot
Match: Q8VXX5 (Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 3.1e-152
Identity = 265/557 (47.58%), Postives = 373/557 (66.97%), Query Frame = 0

Query: 4   LTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINST 63
           L  + LLC   ++A+S     DP+  + + V+Y T SPLGVPQQ I +NGQFPGP +N+T
Sbjct: 7   LLASFLLCFALLSAVSF--AADPFVSYDFRVSYLTASPLGVPQQVIAVNGQFPGPLLNAT 66

Query: 64  SNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGS 123
           +N N+V+NVFN+LDEP LL W G+Q R+NSWQDG+LGTNCPIPP  NFTY FQVKDQIGS
Sbjct: 67  TNYNVVVNVFNHLDEPLLLTWPGIQMRRNSWQDGVLGTNCPIPPRWNFTYQFQVKDQIGS 126

Query: 124 FFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSG 183
           FFY PS    RA G FG + IN+R +IP+P+  P+ +   +IGDWYT+ H  L++ LDSG
Sbjct: 127 FFYSPSLNFQRASGGFGPIVINNRDIIPIPFPQPDGELIFIIGDWYTQDHKALRRALDSG 186

Query: 184 RSIARPDGVLINGK------VAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGH 243
           + +  PDGVLINGK       +  DG D   F ++PGKTY+ R+ NVG+ TSLNFRIQ H
Sbjct: 187 KELGMPDGVLINGKGPYKYNSSVPDGIDYLTFHVEPGKTYRIRVHNVGISTSLNFRIQNH 246

Query: 244 TMKLVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQD-PKDYYMVASTRFTKNI----LV 303
           ++ LVE EG +T Q  +   DVHVGQ +S LVT DQD   DYY+VAS RF        + 
Sbjct: 247 SLLLVETEGHYTSQANFTDFDVHVGQSYSFLVTMDQDATSDYYIVASARFVNETVWQRVT 306

Query: 304 GKGIVRYTNGKGPVSH--EIPEAPMGWTWS-LNQFRTFRWNLTANAARPNPQGSYHYGSI 363
           G  I+ Y+N KGPVS    +P+  +   WS ++Q +T R N +A+ ARPNPQGS+HYG I
Sbjct: 307 GVAILHYSNSKGPVSGPLPVPKTDVSSPWSAMSQPKTIRQNTSASGARPNPQGSFHYGQI 366

Query: 364 NITRTIKIVN-SATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLA 423
           NIT T  + +   T ++G LR  +NG+S V+P TP++LA+  +V    +K D   D    
Sbjct: 367 NITNTYILRSLPPTIINGALRATLNGISFVNPSTPVRLADRNKVKG-AYKLD-FPDRPF- 426

Query: 424 KDGNTVTIAPNVVNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNY 483
                + +  +++NAT++ F++++F+N++  +QS+H+DGYSFF V ++ G W+ +K+ +Y
Sbjct: 427 --NRPLRLDRSMINATYKGFIQVVFQNNDTKIQSFHVDGYSFFVVGMDFGIWSEDKKGSY 486

Query: 484 NLLDAVSRHTIQVFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRD 543
           N  DA+SR TI+V+P  W A+L++LDN G+WN+R E  +  YLG++ Y  + +P    + 
Sbjct: 487 NNWDAISRSTIEVYPGGWTAVLISLDNVGVWNIRVENLDRWYLGEETYMRITNPEEDGKT 546

Query: 544 EYNIPDNTLLCGLVKDM 546
           E + PDN L CG +K++
Sbjct: 547 EMDPPDNVLYCGALKNL 556

BLAST of HG10020495 vs. ExPASy Swiss-Prot
Match: Q9FHN6 (Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=2 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.9e-152
Identity = 255/538 (47.40%), Postives = 367/538 (68.22%), Query Frame = 0

Query: 25  DPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNNNLVINVFNNLDEPFLLHW 84
           DPY  + + ++Y T SPLGVPQQ I +NG+FPGP IN+T+N N+ +NV N+LDEP LL W
Sbjct: 25  DPYVSYDFTLSYITASPLGVPQQVIAVNGKFPGPVINATTNYNVHVNVLNHLDEPLLLTW 84

Query: 85  SGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFYYPSTAMHRAGGAFGGLRI 144
            GVQ R+NSWQDG+LGTNCPIPP  NFTY FQ+KDQIGS+FY PS    RA G FG L I
Sbjct: 85  PGVQMRRNSWQDGVLGTNCPIPPNWNFTYDFQLKDQIGSYFYSPSLNFQRASGGFGALII 144

Query: 145 NSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSIARPDGVLINGK------V 204
           N+R L+P+P+ +P+ +   +IGDWYT++H+ L++ LDSG+ +  PDGVLINGK       
Sbjct: 145 NNRDLVPIPFTEPDGEIIFIIGDWYTQNHTALRRILDSGKELGMPDGVLINGKGPFKYNS 204

Query: 205 AKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEGSHTVQNVYQSLD 264
           +  DG +     + PGKTY+ R+ NVG+ TSLNFRIQ H + L+E EG +T Q  +   D
Sbjct: 205 SVPDGIEHETVNVDPGKTYRIRVHNVGISTSLNFRIQNHKLLLIETEGRYTSQMNFTDFD 264

Query: 265 VHVGQCFSVLVTADQD-PKDYYMVASTRFTKNI----LVGKGIVRYTNGKGPVSHEIP-- 324
           VHVGQ +S LVT DQ+   DYY+VAS RF        + G GI+ Y+N KGP S  +P  
Sbjct: 265 VHVGQSYSFLVTMDQNATSDYYIVASARFVNETVWQRVTGVGILHYSNSKGPASGPLPVS 324

Query: 325 EAPMGWTWS-LNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVN-SATKVDGKLR 384
              +   WS +NQ R  + N +A+ ARPNPQGS+HYG INITRT  + +   TK++GKLR
Sbjct: 325 ATDVNHPWSAMNQPRAIKQNTSASGARPNPQGSFHYGQINITRTYILRSLPPTKINGKLR 384

Query: 385 YAINGVSHVDPETPLKLAEFFEV-ADKVFKY-DTISDEGLAKDGNTVTIAPNVVNATFRN 444
             +NG+S V+P TP++LA+  +V  D +  + D   DE L +      ++ +++NAT++ 
Sbjct: 385 ATLNGISFVNPSTPMRLADDHKVKGDYMLDFPDRPLDEKLPR------LSSSIINATYKG 444

Query: 445 FVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSWA 504
           F+++IF+N++  +QS+H+DGY+F+ VA++ G W+ ++  +YN  DAV+R T++V+P +W 
Sbjct: 445 FIQVIFQNNDTKIQSFHIDGYAFYVVAMDFGIWSEDRNSSYNNWDAVARSTVEVYPGAWT 504

Query: 505 AILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDM 546
           A+L++LDN G+WN+R E  +  YLGQ+ Y  +++P  +   E + P+N + CG ++ M
Sbjct: 505 AVLISLDNVGVWNIRVENLDRWYLGQETYMRIINPEENGSTEMDPPENVMYCGALQAM 556

BLAST of HG10020495 vs. ExPASy TrEMBL
Match: A0A5A7T1B7 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold112G00010 PE=3 SV=1)

HSP 1 Score: 1109.0 bits (2867), Expect = 0.0e+00
Identity = 525/553 (94.94%), Postives = 536/553 (96.93%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLTFTLLLCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVLIGDWYTKS+STLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSYSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK+AKGDGTDEPLFTM PGKTYKYRICNVGLKTSLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKTSLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDP+DYYMVAS+RFTKNILVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK P S EIPEAP GW WSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           AT VDGKLRYAINGVSH+DPETPLKLAE+FEVADKVFKYDTISDEG AK  NTVTIAPNV
Sbjct: 361 ATNVDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQLYFSV SPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of HG10020495 vs. ExPASy TrEMBL
Match: A0A5D3BIA5 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00010 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 524/553 (94.76%), Postives = 535/553 (96.75%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLTFTLLLCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK+AKGDGTDEPLFTM PGKTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDP+DYYMVAS+RFTKNILVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK P S EIPEAP GW WSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           AT +DGKLRYAINGVSH+DPETPLKLAE+FEVADKVFKYDTISDEG AK  NTVTIAPNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQLYFSV SPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of HG10020495 vs. ExPASy TrEMBL
Match: A0A1S3AX96 (L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103483330 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 524/553 (94.76%), Postives = 535/553 (96.75%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARLTFTLLLCLL VAAM TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLTFTLLLCLLLVAAMPTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRAGGAFGGLR+NSRLLIPVPY DPEDDYTVLIGDWYTKSHSTLKKFL
Sbjct: 121 IGSFFYYPSTAMHRAGGAFGGLRVNSRLLIPVPYVDPEDDYTVLIGDWYTKSHSTLKKFL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK+AKGDGTDEPLFTM PGKTYKYRICNVGLK SLNFRIQGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKIAKGDGTDEPLFTMNPGKTYKYRICNVGLKISLNFRIQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDP+DYYMVAS+RFTKNILVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPRDYYMVASSRFTKNILVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           NGK P S EIPEAP GW WSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS
Sbjct: 301 NGKAPASPEIPEAPRGWAWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           AT +DGKLRYAINGVSH+DPETPLKLAE+FEVADKVFKYDTISDEG AK  NTVTIAPNV
Sbjct: 361 ATNIDGKLRYAINGVSHIDPETPLKLAEYFEVADKVFKYDTISDEGSAKTANTVTIAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKRKNYNLLDAVSRHTIQ
Sbjct: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRKNYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSW AILLTLDNAGMWNLRSELTENRYLGQQLYFSV SPARSLRDEYNIPDN LLCG
Sbjct: 481 VFPKSWGAILLTLDNAGMWNLRSELTENRYLGQQLYFSVQSPARSLRDEYNIPDNALLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDM LP+PYTI
Sbjct: 541 LVKDMSLPQPYTI 553

BLAST of HG10020495 vs. ExPASy TrEMBL
Match: A0A5A7TDG2 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold366G001010 PE=3 SV=1)

HSP 1 Score: 1077.0 bits (2784), Expect = 0.0e+00
Identity = 505/553 (91.32%), Postives = 529/553 (95.66%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARL FT LLCL + A M+TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NSTSNNNLVINVFNNLDEPFLLHWSG+QHRKNSWQDGLLGTNCPI PGTNFTYHFQVKDQ
Sbjct: 61  NSTSNNNLVINVFNNLDEPFLLHWSGIQHRKNSWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFR QGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQN YQSLDVHVGQCFSVLVTA+Q+ KDYYMVASTRF KN+LVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           +GKGP S EIPEAP+GW WSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPVGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           A+KVDGKLRYAINGVSHVDPETPLKLAE+F +ADKVFKYDTISDEGLA+   TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of HG10020495 vs. ExPASy TrEMBL
Match: A0A5D3DDJ5 (L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G001190 PE=3 SV=1)

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 505/553 (91.32%), Postives = 528/553 (95.48%), Query Frame = 0

Query: 1   MARLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60
           MARL FT LLCL + A M+TVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI
Sbjct: 1   MARLMFTALLCLSAAAMMATVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNI 60

Query: 61  NSTSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQ 120
           NST+NNNLVINVFNNLDEPFLLHWSGVQHRKN WQDGLLGTNCPI PGTNFTYHFQVKDQ
Sbjct: 61  NSTTNNNLVINVFNNLDEPFLLHWSGVQHRKNCWQDGLLGTNCPIQPGTNFTYHFQVKDQ 120

Query: 121 IGSFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFL 180
           IGSFFYYPSTAMHRA G FGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSH+TLK++L
Sbjct: 121 IGSFFYYPSTAMHRAAGGFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHTTLKQYL 180

Query: 181 DSGRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMK 240
           DSGRSIARPDGVLINGK AKGDGTDEPLFTMKPGKTYKYR+CNVGLK+SLNFR QGHTMK
Sbjct: 181 DSGRSIARPDGVLINGKTAKGDGTDEPLFTMKPGKTYKYRVCNVGLKSSLNFRFQGHTMK 240

Query: 241 LVEMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYT 300
           LVEMEGSHTVQN YQSLDVHVGQCFSVLVTA+Q+ KDYYMVASTRF KN+LVGKGIVRYT
Sbjct: 241 LVEMEGSHTVQNDYQSLDVHVGQCFSVLVTANQESKDYYMVASTRFIKNVLVGKGIVRYT 300

Query: 301 NGKGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNS 360
           +GKGP S EIPEAPMGW WSLNQFRTFRWNLTA+AARPNPQGSYHYGSINITRTIK+VNS
Sbjct: 301 DGKGPASPEIPEAPMGWAWSLNQFRTFRWNLTASAARPNPQGSYHYGSINITRTIKLVNS 360

Query: 361 ATKVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNV 420
           A+KVDGKLRYAINGVSHVDPETPLKLAE+F +ADKVFKYDTISDEGLA+   TVT+APNV
Sbjct: 361 ASKVDGKLRYAINGVSHVDPETPLKLAEYFGIADKVFKYDTISDEGLAEGATTVTVAPNV 420

Query: 421 VNATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQ 480
           VNATFR+F+EIIFENHEKSLQSWHLDGYSFFAVAIEPGRW+PEKR NYNLLDAVSRHTIQ
Sbjct: 421 VNATFRDFIEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWSPEKRANYNLLDAVSRHTIQ 480

Query: 481 VFPKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 540
           VFPKSWAAILLT DNAGMWNLRSELTENRYLG QLY SVLSPARSLRDEYNIPDNTLLCG
Sbjct: 481 VFPKSWAAILLTFDNAGMWNLRSELTENRYLGSQLYISVLSPARSLRDEYNIPDNTLLCG 540

Query: 541 LVKDMPLPKPYTI 554
           LVKDMP+PKPYTI
Sbjct: 541 LVKDMPMPKPYTI 553

BLAST of HG10020495 vs. TAIR 10
Match: AT3G13390.1 (SKU5 similar 11 )

HSP 1 Score: 903.7 bits (2334), Expect = 7.5e-263
Identity = 409/545 (75.05%), Postives = 468/545 (85.87%), Query Frame = 0

Query: 8   LLLCL-LSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNN 67
           L  CL L+ AA   VR EDPYF   WNVTYGT+SPLGVPQQ ILINGQFPGPN+NSTSNN
Sbjct: 7   LAACLYLAAAATVVVRAEDPYFHHVWNVTYGTVSPLGVPQQVILINGQFPGPNVNSTSNN 66

Query: 68  NLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFY 127
           N++INVFNNLDEPFLL W+G+QHRKN WQDG  GT CPI PGTN+TYHFQ KDQIGS+FY
Sbjct: 67  NVIINVFNNLDEPFLLTWNGIQHRKNCWQDGTPGTMCPIMPGTNYTYHFQPKDQIGSYFY 126

Query: 128 YPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSI 187
           YPSTAMHR+ G FGGLR+NSRLLIPVPYADPEDDYTVLIGDWYTKSH+ LKKFLDSGR++
Sbjct: 127 YPSTAMHRSAGGFGGLRVNSRLLIPVPYADPEDDYTVLIGDWYTKSHTQLKKFLDSGRTL 186

Query: 188 ARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 247
            RPDG+LINGK  KGDG+D PLFT+KPGKTY+ RICNVGLKTSLNFRIQ H +KLVEMEG
Sbjct: 187 GRPDGILINGKSGKGDGSDAPLFTLKPGKTYRVRICNVGLKTSLNFRIQNHKLKLVEMEG 246

Query: 248 SHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNGKGPV 307
           SH +QN Y SLDVHVGQC+  ++TA+Q+ KDYYMVAS+RF K+++   G++RY  GKGP 
Sbjct: 247 SHVLQNDYDSLDVHVGQCYGTILTANQEAKDYYMVASSRFLKSVITTTGLLRYEGGKGPA 306

Query: 308 SHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSATKVDG 367
           S ++P  P+GW WSLNQFR+FRWNLTA+AARPNPQGSYHYG INITRTIK+VN+  KVDG
Sbjct: 307 SSQLPPGPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDG 366

Query: 368 KLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVNATFR 427
           KLRYA+NGVSH DPETPLKLAE+F VADKVFKYD+I+D    +   ++ I PNV+N T R
Sbjct: 367 KLRYALNGVSHTDPETPLKLAEYFGVADKVFKYDSITDNPTPEQIKSIKIVPNVLNITHR 426

Query: 428 NFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSW 487
            F+E++FENHEKS+QSWHLDGYSFFAVA+EPG WTPEKRKNYNLLDAVSRHT+QV+PK W
Sbjct: 427 TFIEVVFENHEKSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 486

Query: 488 AAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMP 547
           AAILLT DN GMWN+RSE +E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK  P
Sbjct: 487 AAILLTFDNCGMWNVRSENSERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGTP 546

Query: 548 LPKPY 552
            P PY
Sbjct: 547 KPNPY 551

BLAST of HG10020495 vs. TAIR 10
Match: AT1G55570.1 (SKU5 similar 12 )

HSP 1 Score: 899.0 bits (2322), Expect = 1.8e-261
Identity = 409/545 (75.05%), Postives = 463/545 (84.95%), Query Frame = 0

Query: 8   LLLCL-LSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNN 67
           L +CL ++ A +  V+ EDPYF   WNVTYGT SPLGVPQQ ILINGQFPGPNINSTSNN
Sbjct: 8   LAVCLCVATATVMMVQAEDPYFHHVWNVTYGTASPLGVPQQVILINGQFPGPNINSTSNN 67

Query: 68  NLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFY 127
           N+++NVFNNLDEPFL+ W+G+QHRKN WQDG  GT CPIPPG NFTYHFQ KDQIGS+FY
Sbjct: 68  NVIVNVFNNLDEPFLITWAGIQHRKNCWQDGTAGTMCPIPPGQNFTYHFQPKDQIGSYFY 127

Query: 128 YPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSI 187
           YP+TAMHRA G FGGLR+NSRLLIPVPYADPEDDYT+LI DWYTKSH+ LKKFLDSGR+I
Sbjct: 128 YPTTAMHRAAGGFGGLRVNSRLLIPVPYADPEDDYTILINDWYTKSHTQLKKFLDSGRTI 187

Query: 188 ARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 247
            RPDG+LINGK  K DG+D+PLFT+KPGKTY+ RICNVGLK SLNFRIQ H MKLVEMEG
Sbjct: 188 GRPDGILINGKSGKTDGSDKPLFTLKPGKTYRVRICNVGLKASLNFRIQNHKMKLVEMEG 247

Query: 248 SHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNGKGPV 307
           SH +QN Y SLDVHVGQCF V+VTADQ+PKDYYM+ASTRF K  L   G++RY  GKGP 
Sbjct: 248 SHVLQNDYDSLDVHVGQCFGVIVTADQEPKDYYMIASTRFLKKPLTTTGLLRYEGGKGPA 307

Query: 308 SHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSATKVDG 367
           S ++P AP+GW WSLNQ+R+FRWNLTA+AARPNPQGSYHYG INITRTIK+VN+  KVDG
Sbjct: 308 SSQLPAAPVGWAWSLNQYRSFRWNLTASAARPNPQGSYHYGKINITRTIKLVNTQGKVDG 367

Query: 368 KLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVNATFR 427
           KLRYA++GVSH DPETPLKLAE+F VADKVFKYDTISD         + I PNV+N T R
Sbjct: 368 KLRYALSGVSHTDPETPLKLAEYFGVADKVFKYDTISDNPNPDQIKNIKIEPNVLNITHR 427

Query: 428 NFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSW 487
            F+E++FENHE+S+QSWHLDGYSFFAVA+EPG WTPEKRKNYNLLDAVSRHT+QV+PK W
Sbjct: 428 TFIEVVFENHERSVQSWHLDGYSFFAVAVEPGTWTPEKRKNYNLLDAVSRHTVQVYPKCW 487

Query: 488 AAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMP 547
           AAILLT DN GMWN+RSE  E RYLGQQLY SVLSP +SLRDEYN+P+ +L CGLVK  P
Sbjct: 488 AAILLTFDNCGMWNIRSENAERRYLGQQLYASVLSPEKSLRDEYNMPETSLQCGLVKGKP 547

Query: 548 LPKPY 552
              PY
Sbjct: 548 KVNPY 552

BLAST of HG10020495 vs. TAIR 10
Match: AT3G13400.1 (SKU5 similar 13 )

HSP 1 Score: 875.2 bits (2260), Expect = 2.8e-254
Identity = 406/547 (74.22%), Postives = 463/547 (84.64%), Query Frame = 0

Query: 7   TLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNN 66
           T+L+CL S  A+  V   DPYF++TWNVTYGT +PLG+PQQ ILINGQFPGPN+NSTSNN
Sbjct: 8   TVLVCLASTVAL--VSAGDPYFYYTWNVTYGTAAPLGIPQQVILINGQFPGPNLNSTSNN 67

Query: 67  NLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFY 126
           N+VINVFNNLDEPFLL WSG+QHRKNSWQDG+ GT+CPIP GTNFTYHFQ KDQIGS+FY
Sbjct: 68  NVVINVFNNLDEPFLLTWSGLQHRKNSWQDGVTGTSCPIPAGTNFTYHFQPKDQIGSYFY 127

Query: 127 YPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSI 186
           YPSTA+HR  G FGGLR+NSRLLIPVPYADPEDD T+LI DWY KSH+ LK FLDSGR++
Sbjct: 128 YPSTALHRFAGGFGGLRVNSRLLIPVPYADPEDDRTILINDWYAKSHTALKNFLDSGRTL 187

Query: 187 ARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 246
             PDGVLINGK  K  G + PLFTMKPGKTYKYRICNVG K++LNFRIQGH MKLVEMEG
Sbjct: 188 GSPDGVLINGKSGKLGGNNAPLFTMKPGKTYKYRICNVGFKSTLNFRIQGHKMKLVEMEG 247

Query: 247 SHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNGKGPV 306
           SH +QN Y SLDVHVGQCF+VLVTADQ  K+YYMVASTRF K  +   G++ Y       
Sbjct: 248 SHVLQNDYDSLDVHVGQCFAVLVTADQVAKNYYMVASTRFLKKEVSTVGVMSYEGSNVQA 307

Query: 307 SHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSATKVDG 366
           S +IP+AP+GW WSLNQFR+FRWNLTA+AARPNPQGSYHYG INITRTIK+ N+   V+G
Sbjct: 308 SSDIPKAPVGWAWSLNQFRSFRWNLTASAARPNPQGSYHYGKINITRTIKLANTKNLVNG 367

Query: 367 KLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVNATFR 426
           K+R+  NGVSHVD ETPLKLAE+F +++KVFKY+ I DE  AK   T+T+ PNV+N TFR
Sbjct: 368 KVRFGFNGVSHVDTETPLKLAEYFGMSEKVFKYNVIKDEPAAKI-TTLTVEPNVLNITFR 427

Query: 427 NFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSW 486
            FVE++FENHEKS+QS+HLDGYSFFAVA EPGRWTPEKR NYNLLDAVSRHT+QV+PKSW
Sbjct: 428 TFVEVVFENHEKSMQSFHLDGYSFFAVASEPGRWTPEKRNNYNLLDAVSRHTVQVYPKSW 487

Query: 487 AAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMP 546
           +AILLT DNAGMWN+RSE  E RYLGQQLY SVLSP +SLRDEYNIP NT LCG+VK +P
Sbjct: 488 SAILLTFDNAGMWNIRSENWERRYLGQQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLP 547

Query: 547 LPKPYTI 554
           LP PYTI
Sbjct: 548 LPTPYTI 551

BLAST of HG10020495 vs. TAIR 10
Match: AT1G55560.1 (SKU5 similar 14 )

HSP 1 Score: 870.2 bits (2247), Expect = 9.2e-253
Identity = 402/546 (73.63%), Postives = 464/546 (84.98%), Query Frame = 0

Query: 7   TLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINSTSNN 66
           T+L+CL+S  A+  V   DPYFF TWNVTYGT SPLGVPQ+ ILINGQFPGPN+NSTSNN
Sbjct: 7   TVLVCLVSTVAI--VNAGDPYFFHTWNVTYGTASPLGVPQKVILINGQFPGPNLNSTSNN 66

Query: 67  NLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIGSFFY 126
           N+VINVFN+LDEPFLL WSG+QHRKN WQDG+ GT+CPIP G NFTYHFQ KDQIGS+FY
Sbjct: 67  NVVINVFNHLDEPFLLTWSGIQHRKNCWQDGVAGTSCPIPAGQNFTYHFQPKDQIGSYFY 126

Query: 127 YPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDSGRSI 186
           YP+T++HR  G FGGLR+NSRLLIPVPYADPEDDYTVL+GDWYT  H+ LK FLDSGR++
Sbjct: 127 YPTTSLHRFAGGFGGLRVNSRLLIPVPYADPEDDYTVLLGDWYTAGHTALKNFLDSGRTL 186

Query: 187 ARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLVEMEG 246
             P+GVLINGK  K  G +EPLFTMKPGKTYKYR+CNVG K++LNFRIQ H MKLVEMEG
Sbjct: 187 GLPNGVLINGKSGKVGGKNEPLFTMKPGKTYKYRLCNVGFKSTLNFRIQNHKMKLVEMEG 246

Query: 247 SHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNGKGPV 306
           SH +QN Y SLDVHVGQCFSVLVTA+Q  KDYYMVASTRF K  L   G++RY       
Sbjct: 247 SHVIQNDYDSLDVHVGQCFSVLVTANQAAKDYYMVASTRFLKKELSTVGVIRYEGSNVQA 306

Query: 307 SHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSATKVDG 366
           S E+P+AP+GW WSLNQFR+FRWNLT+NAARPNPQGSYHYG INITR+IK+VNS + VDG
Sbjct: 307 STELPKAPVGWAWSLNQFRSFRWNLTSNAARPNPQGSYHYGKINITRSIKLVNSKSVVDG 366

Query: 367 KLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVNATFR 426
           K+R+  NGVSHVD ETPLKLAE+F++++KVFKY+ I DE  AK    +T+ PNV+N TFR
Sbjct: 367 KVRFGFNGVSHVDTETPLKLAEYFQMSEKVFKYNVIKDEPAAKI-TALTVQPNVLNITFR 426

Query: 427 NFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVFPKSW 486
            FVEIIFENHEK++QS+HLDGYSFFAVA EPGRWTPEKR+NYNLLDAVSRHT+QV+PKSW
Sbjct: 427 TFVEIIFENHEKTMQSFHLDGYSFFAVASEPGRWTPEKRENYNLLDAVSRHTVQVYPKSW 486

Query: 487 AAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCGLVKDMP 546
           +AILLT DNAGMWN+RSE  E +YLG+QLY SVLSP +SLRDEYNIP NT LCG+VK +P
Sbjct: 487 SAILLTFDNAGMWNIRSENLERKYLGEQLYVSVLSPEKSLRDEYNIPLNTNLCGIVKGLP 546

Query: 547 LPKPYT 553
           LP  Y+
Sbjct: 547 LPAHYS 549

BLAST of HG10020495 vs. TAIR 10
Match: AT1G21860.1 (SKU5 similar 7 )

HSP 1 Score: 651.4 bits (1679), Expect = 6.7e-187
Identity = 309/538 (57.43%), Postives = 391/538 (72.68%), Query Frame = 0

Query: 3   RLTFTLLLCLLSVAAMSTVRGEDPYFFFTWNVTYGTISPLGVPQQGILINGQFPGPNINS 62
           R   T LL L+S+A       EDPY FF W+VTYG ISPLGV QQGILING+FPGP+I S
Sbjct: 9   RAMITTLLFLISLAF-----AEDPYRFFEWHVTYGNISPLGVAQQGILINGKFPGPDIIS 68

Query: 63  TSNNNLVINVFNNLDEPFLLHWSGVQHRKNSWQDGLLGTNCPIPPGTNFTYHFQVKDQIG 122
            +N+NL+INVFN+LDEPFLL W+G+++ KNS+QDG+ GT CPIPPG N+TY  QVKDQIG
Sbjct: 69  ITNDNLIINVFNHLDEPFLLSWNGIRNWKNSFQDGVYGTMCPIPPGKNYTYALQVKDQIG 128

Query: 123 SFFYYPSTAMHRAGGAFGGLRINSRLLIPVPYADPEDDYTVLIGDWYTKSHSTLKKFLDS 182
           SF+Y+PS   H+A G FGG+RI+SR LIPVP+  P DDYT+L+GDWY  +H  LK  LD+
Sbjct: 129 SFYYFPSLGFHKAAGGFGGIRISSRALIPVPFPTPADDYTLLVGDWYKTNHKDLKAQLDN 188

Query: 183 GRSIARPDGVLINGKVAKGDGTDEPLFTMKPGKTYKYRICNVGLKTSLNFRIQGHTMKLV 242
           G  +  PDG+LING+      +      ++PGKTY+ RI NVGL+ SLNFRIQ HTMKLV
Sbjct: 189 GGKLPLPDGILINGR------SSGATLNIEPGKTYRLRISNVGLQNSLNFRIQNHTMKLV 248

Query: 243 EMEGSHTVQNVYQSLDVHVGQCFSVLVTADQDPKDYYMVASTRFTKNILVGKGIVRYTNG 302
           E+EG +T+QN++ SLDVHVGQ +SVL+TADQ  KDYY+V S+RFT  IL   G++ Y+N 
Sbjct: 249 EVEGRYTIQNLFSSLDVHVGQSYSVLITADQPAKDYYVVVSSRFTSKILTTTGVLHYSNS 308

Query: 303 KGPVSHEIPEAPMGWTWSLNQFRTFRWNLTANAARPNPQGSYHYGSINITRTIKIVNSAT 362
             PVS  IP+ P+  +WS NQ R  R NLTA+  RPNPQGSY YG INITRTI++ N+  
Sbjct: 309 VAPVSGPIPDGPIKLSWSFNQARAIRTNLTASGPRPNPQGSYRYGVINITRTIRLANNLG 368

Query: 363 KVDGKLRYAINGVSHVDPETPLKLAEFFEVADKVFKYDTISDEGLAKDGNTVTIAPNVVN 422
            ++GK RYA+N  S    +TPLKL ++F++ D V+K  +ISD+        +    +V+ 
Sbjct: 369 HIEGKQRYAVNSASFYPADTPLKLVDYFKI-DGVYKPGSISDQ---PTNGAIFPTTSVMQ 428

Query: 423 ATFRNFVEIIFENHEKSLQSWHLDGYSFFAVAIEPGRWTPEKRKNYNLLDAVSRHTIQVF 482
           A FR FVE+IFEN E  +QSWHLDGYSF+ V +E G+W+P  RK YNL DA+ R TIQV+
Sbjct: 429 ADFRAFVEVIFENSEDIVQSWHLDGYSFYVVGMELGKWSPASRKVYNLNDAILRCTIQVY 488

Query: 483 PKSWAAILLTLDNAGMWNLRSELTENRYLGQQLYFSVLSPARSLRDEYNIPDNTLLCG 541
           P+SW AI + LDN GMWN+RSE+ E +YLGQQ Y  V + + SLRDEY IP N LLCG
Sbjct: 489 PRSWTAIYIALDNVGMWNMRSEIWERQYLGQQFYMRVYTTSTSLRDEYLIPKNALLCG 531

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0036016.10.0e+0094.94L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
XP_008438536.10.0e+0094.76PREDICTED: L-ascorbate oxidase homolog [Cucumis melo] >TYJ98816.1 L-ascorbate ox... [more]
XP_004137383.10.0e+0093.67L-ascorbate oxidase homolog [Cucumis sativus] >KAE8652494.1 hypothetical protein... [more]
KAA0040126.10.0e+0091.32L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
TYK21747.10.0e+0091.32L-ascorbate oxidase-like protein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q006248.3e-25974.36L-ascorbate oxidase homolog OS=Brassica napus OX=3708 GN=Bp10 PE=2 SV=1[more]
P291621.6e-23868.54L-ascorbate oxidase homolog OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SU402.6e-15948.84Monocopper oxidase-like protein SKU5 OS=Arabidopsis thaliana OX=3702 GN=SKU5 PE=... [more]
Q8VXX53.1e-15247.58Monocopper oxidase-like protein SKS1 OS=Arabidopsis thaliana OX=3702 GN=SKS1 PE=... [more]
Q9FHN68.9e-15247.40Monocopper oxidase-like protein SKS2 OS=Arabidopsis thaliana OX=3702 GN=SKS2 PE=... [more]
Match NameE-valueIdentityDescription
A0A5A7T1B70.0e+0094.94L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3BIA50.0e+0094.76L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3AX960.0e+0094.76L-ascorbate oxidase homolog OS=Cucumis melo OX=3656 GN=LOC103483330 PE=3 SV=1[more]
A0A5A7TDG20.0e+0091.32L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5D3DDJ50.0e+0091.32L-ascorbate oxidase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
AT3G13390.17.5e-26375.05SKU5 similar 11 [more]
AT1G55570.11.8e-26175.05SKU5 similar 12 [more]
AT3G13400.12.8e-25474.22SKU5 similar 13 [more]
AT1G55560.19.2e-25373.63SKU5 similar 14 [more]
AT1G21860.16.7e-18757.43SKU5 similar 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 33..147
e-value: 3.0E-39
score: 133.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 29..148
e-value: 3.4E-33
score: 116.4
IPR008972CupredoxinGENE3D2.60.40.420coord: 157..337
e-value: 1.3E-36
score: 128.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 347..546
e-value: 1.5E-34
score: 121.5
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 24..173
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 156..338
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 349..539
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 385..521
e-value: 1.1E-24
score: 86.7
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 159..291
e-value: 2.9E-41
score: 141.2
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 4..544
NoneNo IPR availablePANTHERPTHR11709:SF246L-ASCORBATE OXIDASE-LIKE PROTEINcoord: 4..544
IPR034273Ascorbate oxidase homologue, first cupredoxin domainCDDcd13846CuRO_1_AAO_like_1coord: 29..145
e-value: 3.41469E-80
score: 245.009
IPR034271Ascorbate oxidase homologue, second cupredoxin domainCDDcd13872CuRO_2_AAO_like_1coord: 159..299
e-value: 6.30408E-88
score: 265.806

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020495.1HG10020495.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity