HG10020479 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020479
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCalcium-binding protein
LocationChr04: 32152196 .. 32152792 (-)
RNA-Seq ExpressionHG10020479
SyntenyHG10020479
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGCGGTGGGCTCAGACGTTCCCCGACCCCGCCTGACAAAGAAACCGTCGTCTTCGTCTTCGTCGTTCCGGCTTCGGTGCCCGAGCCTGAACTCCCTTCGGCTGCTTCGCATATTTGACATGTTCGACAAGAACGGGGACGGCATGATCACGACGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGAGCTGGATTCGACGGTGAACTCCTTCATCGAACCGGGAAATGTGGGCCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGTCCTTAAATGAGACCTATTTCGGGGCGGGGATGGTGGAGGAGGATGAGACGGAAACTAGCATGTCGTCGATGTCGCAGGAAGAGTCGGATCTATCGGAGGCATTCAGAGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGACCTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCGGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

mRNA sequence

ATGGAAGCGGTGGGCTCAGACGTTCCCCGACCCCGCCTGACAAAGAAACCGTCGTCTTCGTCTTCGTCGTTCCGGCTTCGGTGCCCGAGCCTGAACTCCCTTCGGCTGCTTCGCATATTTGACATGTTCGACAAGAACGGGGACGGCATGATCACGACGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGAGCTGGATTCGACGGTGAACTCCTTCATCGAACCGGGAAATGTGGGCCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGTCCTTAAATGAGACCTATTTCGGGGCGGGGATGGTGGAGGAGGATGAGACGGAAACTAGCATGTCGTCGATGTCGCAGGAAGAGTCGGATCTATCGGAGGCATTCAGAGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGACCTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCGGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

Coding sequence (CDS)

ATGGAAGCGGTGGGCTCAGACGTTCCCCGACCCCGCCTGACAAAGAAACCGTCGTCTTCGTCTTCGTCGTTCCGGCTTCGGTGCCCGAGCCTGAACTCCCTTCGGCTGCTTCGCATATTTGACATGTTCGACAAGAACGGGGACGGCATGATCACGACGGAGGAGCTGGGTCAAGCTCTGGGGCTTCTTGGGTTGGATGCTGATGAATCGGAGCTGGATTCGACGGTGAACTCCTTCATCGAACCGGGAAATGTGGGCCTTAGGTTCGAAGACTTCGCAGCCCTGCACCAGTCCTTAAATGAGACCTATTTCGGGGCGGGGATGGTGGAGGAGGATGAGACGGAAACTAGCATGTCGTCGATGTCGCAGGAAGAGTCGGATCTATCGGAGGCATTCAGAGTATTCGACGAGGATGGAGATGGCTACATATCCGCCACCGACCTCCAAGTGGTTCTAAGAAAGCTGAGATTGGCGGAGGGGAATGAGATCGACAGGGTTCAGAAGATGATTTTGTCGGTTGACCGCAATCAGGATGGCCGGGTTGATTTCTTCGAATTCAAGGACATGATGCGCAGTGTTATCGTCCGGAGTTGTTGA

Protein sequence

MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVIVRSC
Homology
[Close]
BLAST of HG10020479 vs. NCBI nr
Match: XP_008442311.1 (PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa] >TYK24898.1 calcium-binding allergen Bet v 3 [Cucumis melo var. makuwa])

HSP 1 Score: 354.8 bits (909), Expect = 5.0e-94
Identity = 187/200 (93.50%), Postives = 193/200 (96.50%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSSSSSSFRLRCPSLNSLRL RIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE--TETSM 120
           GLLGLDADESELDSTVNSFI+PGNVGLRFEDFAALHQSLN+TYF AGMVE+++   ETSM
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYF-AGMVEDEDEKRETSM 120

Query: 121 SSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQD 180
           SSMSQEESDLSEAFRVFDEDGDGYISAT+LQVVLRKLRLAEGNEIDRVQKMILSVDRNQD
Sbjct: 121 SSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQD 180

Query: 181 GRVDFFEFKDMMRSVIVRSC 199
           GRVDFFEFKDMMRSVIVRSC
Sbjct: 181 GRVDFFEFKDMMRSVIVRSC 199

[Close]
BLAST of HG10020479 vs. NCBI nr
Match: XP_004137668.1 (calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein Csa_002212 [Cucumis sativus])

HSP 1 Score: 352.8 bits (904), Expect = 1.9e-93
Identity = 185/201 (92.04%), Postives = 190/201 (94.53%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSS SSSFRLRCPSLNSLRL RIFDMFDKNGDGMITT+ELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE---TETS 120
           GLLGLDADESELDSTVNSFIEPGN GLRFEDFAALHQSLN+TYFG  + +EDE    ETS
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDEKRETS 120

Query: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180
           MSSMSQEESDLSEAFRVFDEDGDGYISAT+LQVVLRKLRLAEGNEIDRVQKMILSVDRNQ
Sbjct: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180

Query: 181 DGRVDFFEFKDMMRSVIVRSC 199
           DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 DGRVDFFEFKDMMRSVIVRSC 201

[Close]
BLAST of HG10020479 vs. NCBI nr
Match: XP_022982604.1 (calcium-binding allergen Bet v 3-like [Cucurbita maxima])

HSP 1 Score: 338.6 bits (867), Expect = 3.7e-89
Identity = 176/202 (87.13%), Postives = 187/202 (92.57%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ SDVPRPRLTKKPSSSS SFRLRCPSLNSLRL RIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE----TET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLN+TYFG  M +E+E     ET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISAT+LQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 199
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]
BLAST of HG10020479 vs. NCBI nr
Match: XP_023527056.1 (calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 338.2 bits (866), Expect = 4.8e-89
Identity = 176/202 (87.13%), Postives = 187/202 (92.57%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSSSS SFRLRCPSLNSLRL RIFDMFDKNGDGMITTEELGQAL
Sbjct: 70  MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 129

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE----TET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLN+TYFG  + +E+E     ET
Sbjct: 130 GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPVEDEEEKEEKRET 189

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISAT+LQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 190 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 249

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 199
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 250 RDGRVDFFEFKDMMRSVIVRSC 271

[Close]
BLAST of HG10020479 vs. NCBI nr
Match: KAG6580420.1 (Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.1 bits (858), Expect = 4.1e-88
Identity = 175/202 (86.63%), Postives = 186/202 (92.08%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSSSS SFRLRCPSLNSLRL RIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE----TET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLN+TYF   + +E+E     ET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFREPVEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISAT+LQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 199
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]
BLAST of HG10020479 vs. ExPASy Swiss-Prot
Match: P43187 (Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 4.4e-61
Identity = 123/185 (66.49%), Postives = 150/185 (81.08%), Query Frame = 0 

Query: 15  KKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           +K S S+SSFRLR  SLN+LRL RIFD+FDKN DG+IT +EL +AL LLGL+ D SEL+S
Sbjct: 20  RKRSLSNSSFRLRSESLNTLRLRRIFDLFDKNSDGIITVDELSRALNLLGLETDLSELES 79

Query: 75  TVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSM--SSMSQEESDLSEAF 134
           TV SF   GN+GL+FEDF +LHQSLN++YF  G  +ED+ E  M  S +SQEE+D    F
Sbjct: 80  TVKSFTREGNIGLQFEDFISLHQSLNDSYFAYGGEDEDDNEEDMRKSILSQEEADSFGGF 139

Query: 135 RVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRS 194
           +VFDEDGDGYISA +LQ+VL KL  +EG+EIDRV+KMI+SVD N+DGRVDFFEFKDMMRS
Sbjct: 140 KVFDEDGDGYISARELQMVLGKLGFSEGSEIDRVEKMIVSVDSNRDGRVDFFEFKDMMRS 199

Query: 195 VIVRS 198
           V+VRS
Sbjct: 200 VLVRS 204

[Close]
BLAST of HG10020479 vs. ExPASy Swiss-Prot
Match: Q9SVG9 (Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.1e-59
Identity = 118/183 (64.48%), Postives = 150/183 (81.97%), Query Frame = 0 

Query: 15  KKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK +  SSSFRLR PSLN+LRL RIFD+FDKNGDG IT EEL QAL  LGL+AD S+L S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRV 134
           TV S+I+PGN GL F+DF++LH++L++++FG G     E E   SS ++ ESDL+EAF+V
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFG-GACGGGENEDDPSSAAENESDLAEAFKV 128

Query: 135 FDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVI 194
           FDE+GDG+ISA +LQ VL+KL L EG E++RV+KMI+SVDRNQDGRVDFFEFK+MMR+V+
Sbjct: 129 FDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRTVV 188

Query: 195 VRS 198
           + S
Sbjct: 189 IPS 190

[Close]
BLAST of HG10020479 vs. ExPASy Swiss-Prot
Match: Q9FI19 (Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 PE=2 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 8.1e-55
Identity = 117/183 (63.93%), Postives = 140/183 (76.50%), Query Frame = 0 

Query: 15  KKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK S  SSSFRLR PSLN+LRL R+FD+FDKN DG IT EEL QAL  LGLDAD S+L S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRV 134
           TV+SFI+P   GLRF+DFAALH++L+E++FG         E S    S  ESDL EAF V
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFGG--------EGSCCDGS-PESDLEEAFNV 127

Query: 135 FDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVI 194
           FDEDGDG+ISA +LQ VL+KL L E  EI++V+KMI+SVD N DGRVDFFEFK+MM++V+
Sbjct: 128 FDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTVV 181

Query: 195 VRS 198
           V S
Sbjct: 188 VPS 181

[Close]
BLAST of HG10020479 vs. ExPASy Swiss-Prot
Match: Q09011 (Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 5.2e-54
Identity = 117/183 (63.93%), Postives = 142/183 (77.60%), Query Frame = 0 

Query: 15  KKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           K   SSSSSFRLR PSLNS+RL RIFD+FD+N D +I+ EEL QAL LLGLDAD SE++S
Sbjct: 20  KLKPSSSSSFRLRSPSLNSIRLRRIFDVFDRNHDCLISVEELSQALNLLGLDADLSEIES 79

Query: 75  TVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRV 134
            V   I+P N GLRFEDF  LH+SLN+ +FG+    ED+   +    +Q+ESDL EAF V
Sbjct: 80  MVKLHIKPENTGLRFEDFETLHRSLNDVFFGSKC--EDKLGLN-PDPAQDESDLKEAFDV 139

Query: 135 FDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVI 194
           FDE+GDG+ISA +LQVVL KL L EG+EIDRV+ MI SV+++ DGRVDFFEFKDMMR+VI
Sbjct: 140 FDENGDGFISAKELQVVLEKLGLPEGSEIDRVEMMISSVEQDHDGRVDFFEFKDMMRTVI 199

Query: 195 VRS 198
           V S
Sbjct: 200 VPS 199

[Close]
BLAST of HG10020479 vs. ExPASy Swiss-Prot
Match: Q10LX4 (Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 GN=CML27 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.3e-44
Identity = 103/195 (52.82%), Postives = 132/195 (67.69%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           M+A G    +P L++KP   S SFRLR  SLN+LRL R+FD+FD+NGDG IT +E+  AL
Sbjct: 1   MDAAGV-AAKPSLSRKP---SPSFRLRNGSLNALRLRRVFDLFDRNGDGEITLDEMASAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSS 120
             LGL AD + L++TV  +I  G  GLRF DF ALH++L +  FG   VEE+  E     
Sbjct: 61  DALGLGADRAGLEATVGGYIPAGAAGLRFGDFEALHRALGDALFGP--VEEE--EPGKQG 120

Query: 121 MSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGR 180
              +E D+ EAFRVFDEDGDG+ISA +LQ VL+KL L E   +  VQ+MI +VDR+ DGR
Sbjct: 121 EDDDEGDMKEAFRVFDEDGDGFISAAELQAVLKKLGLPEARNLATVQEMICNVDRDCDGR 180

Query: 181 VDFFEFKDMMRSVIV 196
           VDF EFK MM+ + V
Sbjct: 181 VDFGEFKCMMQGITV 187

[Close]
BLAST of HG10020479 vs. ExPASy TrEMBL
Match: A0A5A7TQ56 (Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00090 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.4e-94
Identity = 187/200 (93.50%), Postives = 193/200 (96.50%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSSSSSSFRLRCPSLNSLRL RIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE--TETSM 120
           GLLGLDADESELDSTVNSFI+PGNVGLRFEDFAALHQSLN+TYF AGMVE+++   ETSM
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYF-AGMVEDEDEKRETSM 120

Query: 121 SSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQD 180
           SSMSQEESDLSEAFRVFDEDGDGYISAT+LQVVLRKLRLAEGNEIDRVQKMILSVDRNQD
Sbjct: 121 SSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQD 180

Query: 181 GRVDFFEFKDMMRSVIVRSC 199
           GRVDFFEFKDMMRSVIVRSC
Sbjct: 181 GRVDFFEFKDMMRSVIVRSC 199

[Close]
BLAST of HG10020479 vs. ExPASy TrEMBL
Match: A0A1S3B620 (calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.4e-94
Identity = 187/200 (93.50%), Postives = 193/200 (96.50%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSSSSSSFRLRCPSLNSLRL RIFDMFDKNGDGMIT EELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITAEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE--TETSM 120
           GLLGLDADESELDSTVNSFI+PGNVGLRFEDFAALHQSLN+TYF AGMVE+++   ETSM
Sbjct: 61  GLLGLDADESELDSTVNSFIKPGNVGLRFEDFAALHQSLNDTYF-AGMVEDEDEKRETSM 120

Query: 121 SSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQD 180
           SSMSQEESDLSEAFRVFDEDGDGYISAT+LQVVLRKLRLAEGNEIDRVQKMILSVDRNQD
Sbjct: 121 SSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQD 180

Query: 181 GRVDFFEFKDMMRSVIVRSC 199
           GRVDFFEFKDMMRSVIVRSC
Sbjct: 181 GRVDFFEFKDMMRSVIVRSC 199

[Close]
BLAST of HG10020479 vs. ExPASy TrEMBL
Match: A0A0A0LA10 (Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=1)

HSP 1 Score: 352.8 bits (904), Expect = 9.1e-94
Identity = 185/201 (92.04%), Postives = 190/201 (94.53%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSS SSSFRLRCPSLNSLRL RIFDMFDKNGDGMITT+ELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSLSSSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTDELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE---TETS 120
           GLLGLDADESELDSTVNSFIEPGN GLRFEDFAALHQSLN+TYFG  + +EDE    ETS
Sbjct: 61  GLLGLDADESELDSTVNSFIEPGNEGLRFEDFAALHQSLNDTYFGGMVEDEDEDEKRETS 120

Query: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180
           MSSMSQEESDLSEAFRVFDEDGDGYISAT+LQVVLRKLRLAEGNEIDRVQKMILSVDRNQ
Sbjct: 121 MSSMSQEESDLSEAFRVFDEDGDGYISATELQVVLRKLRLAEGNEIDRVQKMILSVDRNQ 180

Query: 181 DGRVDFFEFKDMMRSVIVRSC 199
           DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 DGRVDFFEFKDMMRSVIVRSC 201

[Close]
BLAST of HG10020479 vs. ExPASy TrEMBL
Match: A0A6J1J521 (calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC111481430 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.8e-89
Identity = 176/202 (87.13%), Postives = 187/202 (92.57%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEA+ SDVPRPRLTKKPSSSS SFRLRCPSLNSLRL RIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAMASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE----TET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQSLN+TYFG  M +E+E     ET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQSLNDTYFGEPMEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISAT+LQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 199
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]
BLAST of HG10020479 vs. ExPASy TrEMBL
Match: A0A6J1F9I1 (calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442046 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 9.8e-88
Identity = 174/202 (86.14%), Postives = 185/202 (91.58%), Query Frame = 0 

Query: 1   MEAVGSDVPRPRLTKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQAL 60
           MEAV SDVPRPRLTKKPSSSS SFRLRCPSLNSLRL RIFDMFDKNGDGMITTEELGQAL
Sbjct: 1   MEAVASDVPRPRLTKKPSSSSLSFRLRCPSLNSLRLRRIFDMFDKNGDGMITTEELGQAL 60

Query: 61  GLLGLDADESELDSTVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDE----TET 120
           GLLGLDADES+LDSTVNSFI PGNVGLRFEDFAALHQ LN+TYF   + +E+E     ET
Sbjct: 61  GLLGLDADESDLDSTVNSFIAPGNVGLRFEDFAALHQFLNDTYFREPVEDEEEKEEKRET 120

Query: 121 SMSSMSQEESDLSEAFRVFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRN 180
           +MS +SQEE DLSEAFRVFDEDGDGYISAT+LQVVLRKLRL+EGNE+DRVQKMILSVD N
Sbjct: 121 NMSPVSQEELDLSEAFRVFDEDGDGYISATELQVVLRKLRLSEGNELDRVQKMILSVDSN 180

Query: 181 QDGRVDFFEFKDMMRSVIVRSC 199
           +DGRVDFFEFKDMMRSVIVRSC
Sbjct: 181 RDGRVDFFEFKDMMRSVIVRSC 202

[Close]
BLAST of HG10020479 vs. TAIR 10
Match: AT4G20780.1 (calmodulin like 42 )

HSP 1 Score: 231.1 bits (588), Expect = 7.7e-61
Identity = 118/183 (64.48%), Postives = 150/183 (81.97%), Query Frame = 0 

Query: 15  KKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK +  SSSFRLR PSLN+LRL RIFD+FDKNGDG IT EEL QAL  LGL+AD S+L S
Sbjct: 9   KKVARQSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEELSQALTRLGLNADLSDLKS 68

Query: 75  TVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRV 134
           TV S+I+PGN GL F+DF++LH++L++++FG G     E E   SS ++ ESDL+EAF+V
Sbjct: 69  TVESYIQPGNTGLNFDDFSSLHKTLDDSFFG-GACGGGENEDDPSSAAENESDLAEAFKV 128

Query: 135 FDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVI 194
           FDE+GDG+ISA +LQ VL+KL L EG E++RV+KMI+SVDRNQDGRVDFFEFK+MMR+V+
Sbjct: 129 FDENGDGFISARELQTVLKKLGLPEGGEMERVEKMIVSVDRNQDGRVDFFEFKNMMRTVV 188

Query: 195 VRS 198
           + S
Sbjct: 189 IPS 190

[Close]
BLAST of HG10020479 vs. TAIR 10
Match: AT5G44460.1 (calmodulin like 43 )

HSP 1 Score: 214.9 bits (546), Expect = 5.7e-56
Identity = 117/183 (63.93%), Postives = 140/183 (76.50%), Query Frame = 0 

Query: 15  KKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDS 74
           KK S  SSSFRLR PSLN+LRL R+FD+FDKN DG IT EEL QAL  LGLDAD S+L S
Sbjct: 8   KKLSRQSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEELSQALSRLGLDADFSDLKS 67

Query: 75  TVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRV 134
           TV+SFI+P   GLRF+DFAALH++L+E++FG         E S    S  ESDL EAF V
Sbjct: 68  TVDSFIKPDKTGLRFDDFAALHKTLDESFFGG--------EGSCCDGS-PESDLEEAFNV 127

Query: 135 FDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMRSVI 194
           FDEDGDG+ISA +LQ VL+KL L E  EI++V+KMI+SVD N DGRVDFFEFK+MM++V+
Sbjct: 128 FDEDGDGFISAVELQKVLKKLGLPEAGEIEQVEKMIVSVDSNHDGRVDFFEFKNMMQTVV 181

Query: 195 VRS 198
           V S
Sbjct: 188 VPS 181

[Close]
BLAST of HG10020479 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 109.8 bits (273), Expect = 2.6e-24
Identity = 64/178 (35.96%), Postives = 100/178 (56.18%), Query Frame = 0 

Query: 14  TKKPSSSSSSFRLRCPSLNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELD 73
           T  PS SSSS   +   ++   L R+F MFDKNGDG IT EEL  +L  LG+   + +L 
Sbjct: 46  TMLPSPSSSSAPTK--RIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLT 105

Query: 74  STVNSFIEPGNVGLRFEDFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFR 133
             ++     G+  +  ++F +L+ S+ + +   G  EE+              D+ +AF 
Sbjct: 106 QMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEE--------------DMKDAFN 165

Query: 134 VFDEDGDGYISATDLQVVLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMR 192
           VFD+DGDG+I+  +L+ V+  L L +G  +D  +KMI+ VD + DGRV++ EF  MM+
Sbjct: 166 VFDQDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMMK 207

[Close]
BLAST of HG10020479 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 107.5 bits (267), Expect = 1.3e-23
Identity = 60/161 (37.27%), Postives = 93/161 (57.76%), Query Frame = 0 

Query: 31  LNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDSTVNSFIEPGNVGLRFE 90
           ++S  L R+F MFDK+GDG ITT+EL ++   LG+   E EL   +      G+  +  E
Sbjct: 1   MDSTELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIE 60

Query: 91  DFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRVFDEDGDGYISATDLQV 150
           +F  L++++         + EDE E         E D+ EAF VFD +GDG+I+  +L+ 
Sbjct: 61  EFGELYKTI---------MVEDEDEVG-------EEDMKEAFNVFDRNGDGFITVDELKA 120

Query: 151 VLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMR 192
           VL  L L +G  ++  +KMI+ VD + DGRV++ EF+ MM+
Sbjct: 121 VLSSLGLKQGKTLEECRKMIMQVDVDGDGRVNYMEFRQMMK 145

[Close]
BLAST of HG10020479 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 106.3 bits (264), Expect = 2.9e-23
Identity = 61/161 (37.89%), Postives = 92/161 (57.14%), Query Frame = 0 

Query: 31  LNSLRLLRIFDMFDKNGDGMITTEELGQALGLLGLDADESELDSTVNSFIEPGNVGLRFE 90
           ++   L R+F MFDKNGDG IT +EL + L  LG+   + EL   +      G+  +  +
Sbjct: 1   MDPTELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDID 60

Query: 91  DFAALHQSLNETYFGAGMVEEDETETSMSSMSQEESDLSEAFRVFDEDGDGYISATDLQV 150
           +F  L++++        M EEDE          EE D+ EAF VFD++GDG+I+  +L+ 
Sbjct: 61  EFGELYKTI--------MDEEDE----------EEEDMKEAFNVFDQNGDGFITVDELKA 120

Query: 151 VLRKLRLAEGNEIDRVQKMILSVDRNQDGRVDFFEFKDMMR 192
           VL  L L +G  +D  +KMI  VD + DGRV++ EF+ MM+
Sbjct: 121 VLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEFRQMMK 143

The following BLAST results are available for this feature:
BLAST of HG10020479 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_008442311.15.0e-9493.50PREDICTED: calcium-binding allergen Bet v 3 [Cucumis melo] >KAA0044236.1 calcium... [more]
XP_004137668.11.9e-9392.04calcium-binding protein CML42 [Cucumis sativus] >KGN58678.1 hypothetical protein... [more]
XP_022982604.13.7e-8987.13calcium-binding allergen Bet v 3-like [Cucurbita maxima][more]
XP_023527056.14.8e-8987.13calcium-binding allergen Bet v 3-like [Cucurbita pepo subsp. pepo][more]
KAG6580420.14.1e-8886.63Calcium-binding allergen Bet v 3, partial [Cucurbita argyrosperma subsp. sororia... [more]
BLAST of HG10020479 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P431874.4e-6166.49Calcium-binding allergen Bet v 3 OS=Betula pendula OX=3505 GN=BETVIII PE=1 SV=1[more]
Q9SVG91.1e-5964.48Calcium-binding protein CML42 OS=Arabidopsis thaliana OX=3702 GN=CML42 PE=1 SV=1[more]
Q9FI198.1e-5563.93Probable calcium-binding protein CML43 OS=Arabidopsis thaliana OX=3702 GN=CML43 ... [more]
Q090115.2e-5463.93Calcium-binding protein CAST OS=Solanum tuberosum OX=4113 PE=2 SV=1[more]
Q10LX41.3e-4452.82Probable calcium-binding protein CML27 OS=Oryza sativa subsp. japonica OX=39947 ... [more]
BLAST of HG10020479 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7TQ562.4e-9493.50Calcium-binding allergen Bet v 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B6202.4e-9493.50calcium-binding allergen Bet v 3 OS=Cucumis melo OX=3656 GN=LOC103486221 PE=4 SV... [more]
A0A0A0LA109.1e-9492.04Calcium binding protein/cast OS=Cucumis sativus OX=3659 GN=Csa_3G727960 PE=4 SV=... [more]
A0A6J1J5211.8e-8987.13calcium-binding allergen Bet v 3-like OS=Cucurbita maxima OX=3661 GN=LOC11148143... [more]
A0A6J1F9I19.8e-8886.14calcium-binding allergen Bet v 3-like OS=Cucurbita moschata OX=3662 GN=LOC111442... [more]
BLAST of HG10020479 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G20780.17.7e-6164.48calmodulin like 42 [more]
AT5G44460.15.7e-5663.93calmodulin like 43 [more]
AT2G43290.12.6e-2435.96Calcium-binding EF-hand family protein [more]
AT4G03290.11.3e-2337.27EF hand calcium-binding protein family [more]
AT1G05990.12.9e-2337.89EF hand calcium-binding protein family [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 127..155
e-value: 3.2E-5
score: 33.3
coord: 165..193
e-value: 9.0E-6
score: 35.2
coord: 35..63
e-value: 9.6E-6
score: 35.1
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 126..191
e-value: 4.8E-13
score: 49.4
IPR002048EF-hand domainPFAMPF13405EF-hand_6coord: 36..64
e-value: 8.6E-8
score: 31.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 31..66
score: 15.015522
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 123..158
score: 14.262346
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 161..196
score: 13.592857
IPR002048EF-hand domainCDDcd00051EFhcoord: 127..191
e-value: 5.59378E-15
score: 64.4913
NoneNo IPR availableGENE3D1.10.238.10coord: 100..192
e-value: 5.5E-19
score: 70.2
NoneNo IPR availableGENE3D1.10.238.10coord: 34..99
e-value: 5.6E-13
score: 50.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availablePANTHERPTHR23050:SF356CALCIUM BINDINGcoord: 19..197
NoneNo IPR availablePANTHERPTHR23050CALCIUM BINDING PROTEINcoord: 19..197
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 136..148
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 44..56
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 174..186
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 35..191

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020479.1HG10020479.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding