HG10020425 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10020425 vs. NCBI nr
Match: XP_038905464.1 (dnaJ protein ERDJ3A isoform X1 [Benincasa hispida])

HSP 1 Score: 1033.5 bits (2671), Expect = 7.1e-298
Identity = 540/590 (91.53%), Postives = 550/590 (93.22%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M+ RLASI  IMA LF FL SLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLASITLIMAFLFLFLCSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINNAYEILSDEEKR  FDMYG+EKGSPGFGPGSS DQGGGYTFFT+G G
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGNEKGSPGFGPGSSEDQGGGYTFFTNGAG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNE---G 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK+E   G
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDEGNFG 180

Query: 181 GFGSHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVA 240
            FGSHSKAQSGSRTSSS KVINKQVFKKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVA
Sbjct: 181 SFGSHSKAQSGSRTSSSIKVINKQVFKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 241 SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLK 300
           SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNL+
Sbjct: 241 SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLR 300

Query: 301 SFCQEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYD 360
           SFCQEHFPRFSQRVNLKQFDFSSS+GGRLPTLMLLSTKKDTPVIWRVLSGLFRK FSFYD
Sbjct: 301 SFCQEHFPRFSQRVNLKQFDFSSSDGGRLPTLMLLSTKKDTPVIWRVLSGLFRKQFSFYD 360

Query: 361 AEVTDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420
            EVTDVSDPS+KKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKK
Sbjct: 361 TEVTDVSDPSVKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 421 NRKAASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM 480
           NRKA SRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM
Sbjct: 421 NRKAVSRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM 480

Query: 481 ESYQDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSS 540
                          VSQKTLSRRQN  PGSKET+SYALVDA+KQPSFLNAFD AGFKSS
Sbjct: 481 ---------------VSQKTLSRRQNSTPGSKETISYALVDASKQPSFLNAFDGAGFKSS 540

Query: 541 DKILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           DKILI YKRRKGKFAAH GEITEEEVEKFIGSVLNGDVTFTKTRQKPILK
Sbjct: 541 DKILIAYKRRKGKFAAHLGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 575

BLAST of HG10020425 vs. NCBI nr
Match: TYK24944.1 (dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.7e-289
Identity = 522/587 (88.93%), Postives = 539/587 (91.82%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLASII ++A LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFG G SGDQGGGYTFFT+GGG
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK++G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINKQ+ KKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVAS+L
Sbjct: 181 GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVASTL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS EGSLVEYSGD+ VK+LK FC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN GRLPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           TD SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRP SKTQSDS ENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLE IL M   
Sbjct: 421 AASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSSDKI 540
                       VSQKTLSRRQN  PGSKET+SYALVDATKQ SFLNAFDSAGFKSSDKI
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKI 540

Query: 541 LITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           LI YKRRKGKFAA+ GEITEEEVEKFIGSVLNGDVTFT TRQKPILK
Sbjct: 541 LIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of HG10020425 vs. NCBI nr
Match: XP_008442391.1 (PREDICTED: dnaJ protein ERDJ3A [Cucumis melo] >KAA0044188.1 dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 1000.3 bits (2585), Expect = 6.7e-288
Identity = 522/588 (88.78%), Postives = 539/588 (91.67%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLASII ++A LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFG G SGDQGGGYTFFT+GGG
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK++G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINKQ+ KKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVAS+L
Sbjct: 181 GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVASTL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS EGSLVEYSGD+ VK+LK FC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN GRLPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           TD SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRP SKTQSDS ENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLE IL M   
Sbjct: 421 AASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAF-DSAGFKSSDK 540
                       VSQKTLSRRQN  PGSKET+SYALVDATKQ SFLNAF DSAGFKSSDK
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDK 540

Query: 541 ILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           ILI YKRRKGKFAA+ GEITEEEVEKFIGSVLNGDVTFT TRQKPILK
Sbjct: 541 ILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of HG10020425 vs. NCBI nr
Match: XP_004137701.1 (dnaJ protein ERDJ3A isoform X1 [Cucumis sativus] >KGN58737.1 hypothetical protein Csa_001124 [Cucumis sativus])

HSP 1 Score: 996.1 bits (2574), Expect = 1.3e-286
Identity = 518/587 (88.25%), Postives = 537/587 (91.48%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M+TRLASIIF+MA LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLASIIFVMAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
            KKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFGPGSSGDQGGGYTFFT+GGG
Sbjct: 61  NKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGPGSSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFG EDIFSN FGGGF+ +G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGAEDIFSNLFGGGFQGKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINK+  KKEIVDQGMTWLLFPT S LKG+DHVQS IEEVA+SL
Sbjct: 181 GHSKAQSGPKSSSSIKVINKKSLKKEIVDQGMTWLLFPTASSLKGLDHVQSTIEEVANSL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS+EGSLVEYSGD+AVK+LKSFC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSSEGSLVEYSGDIAVKSLKSFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGGLPTLMLLSTKKETPVIWRVLSGLFRKQFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           T  SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TYASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRPAS+TQSDS ENQIPLL GSNFDSVCGEKTPVCVIGAFRSSKARNKLE ILNM   
Sbjct: 421 AASRPASRTQSDSGENQIPLLAGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILNM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSSDKI 540
                       VSQKTLSRRQN  PGSKETVSYALVDATKQ SFLNAFDSAGFKSSDKI
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETVSYALVDATKQVSFLNAFDSAGFKSSDKI 540

Query: 541 LITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           LI YKRRKGKFAA+  EITEEEVEKFIGSVLNGDVTFTKT QKPILK
Sbjct: 541 LIAYKRRKGKFAAYLDEITEEEVEKFIGSVLNGDVTFTKTHQKPILK 572

BLAST of HG10020425 vs. NCBI nr
Match: XP_038905465.1 (dnaJ protein ERDJ3A isoform X2 [Benincasa hispida])

HSP 1 Score: 986.5 bits (2549), Expect = 1.0e-283
Identity = 522/590 (88.47%), Postives = 531/590 (90.00%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M+ RLASI  IMA LF FL SLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLASITLIMAFLFLFLCSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINN                     GSPGFGPGSS DQGGGYTFFT+G G
Sbjct: 61  KKKGAQQKFEEINN---------------------GSPGFGPGSSEDQGGGYTFFTNGAG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNE---G 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK+E   G
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDEGNFG 180

Query: 181 GFGSHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVA 240
            FGSHSKAQSGSRTSSS KVINKQVFKKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVA
Sbjct: 181 SFGSHSKAQSGSRTSSSIKVINKQVFKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 241 SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLK 300
           SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNL+
Sbjct: 241 SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLR 300

Query: 301 SFCQEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYD 360
           SFCQEHFPRFSQRVNLKQFDFSSS+GGRLPTLMLLSTKKDTPVIWRVLSGLFRK FSFYD
Sbjct: 301 SFCQEHFPRFSQRVNLKQFDFSSSDGGRLPTLMLLSTKKDTPVIWRVLSGLFRKQFSFYD 360

Query: 361 AEVTDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420
            EVTDVSDPS+KKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKK
Sbjct: 361 TEVTDVSDPSVKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKK 420

Query: 421 NRKAASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM 480
           NRKA SRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM
Sbjct: 421 NRKAVSRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM 480

Query: 481 ESYQDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSS 540
                          VSQKTLSRRQN  PGSKET+SYALVDA+KQPSFLNAFD AGFKSS
Sbjct: 481 ---------------VSQKTLSRRQNSTPGSKETISYALVDASKQPSFLNAFDGAGFKSS 540

Query: 541 DKILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           DKILI YKRRKGKFAAH GEITEEEVEKFIGSVLNGDVTFTKTRQKPILK
Sbjct: 541 DKILIAYKRRKGKFAAHLGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 554

BLAST of HG10020425 vs. ExPASy Swiss-Prot
Match: Q9SR96 (DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 7.9e-183
Identity = 346/591 (58.54%), Postives = 440/591 (74.45%), Query Frame = 0

Query: 3   TRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKK 62
           TRLA  + ++++L   LL+++AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKNK 
Sbjct: 4   TRLAISVVLVSTL--LLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKD 63

Query: 63  KGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQ 122
           KGAQ+KF EINNAYEILSDEEKR  +D+YGDEKG PGF  G  G   GGY++ + GGG  
Sbjct: 64  KGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGGYSYSSSGGGFN 123

Query: 123 SQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNE---GGF 182
                GPG WQ+ GG GGS+SFSFSFGGPS S+ FGFGM+DIFS F GG  K +   GGF
Sbjct: 124 FG---GPGGWQNMGGGGGSKSFSFSFGGPSESS-FGFGMDDIFSMFSGGSSKGKEQFGGF 183

Query: 183 GSHSKAQSGSRTS--SSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVA 242
           GS S A+S S++S  ++ K IN QV+KK++VDQGMTWLL       +G  + +SIIEEVA
Sbjct: 184 GSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIEEVA 243

Query: 243 SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLK 302
            SLQGALKVGR++C++ESSLCK LGI P R PR+FVYSY  S + +L EY+ ++  K +K
Sbjct: 244 ESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYTEELVAKKVK 303

Query: 303 SFCQEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYD 362
           SFCQEH PRFS++++L  FD S+ +  + P ++LLSTKKDTPVIWRVLSGL+   F FY+
Sbjct: 304 SFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNGRFVFYN 363

Query: 363 AEVTDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKK 422
            EV D SDP I+KLGVD  PAIVGWLSNGEK VL+TGI VK+LKSA+ +L  LL G EKK
Sbjct: 364 TEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLLEGLEKK 423

Query: 423 NRKAASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM 482
           N+K +S+  +    + +  +IPLL+  NFDS+CGE TPVC+IGAFRSS  + KL+ I++ 
Sbjct: 424 NKKVSSKSQAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEKLQSIMS- 483

Query: 483 ESYQDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFK-S 542
                         +VSQK+LSRRQ    GS++TVSY+L+DATKQ +FL++ D + FK S
Sbjct: 484 --------------KVSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTS 543

Query: 543 SDKILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           SDK+LI YK R+GKFA   G++T EEVEKF+ +VLNGD+ FTKTRQKP +K
Sbjct: 544 SDKLLIAYKPRRGKFATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of HG10020425 vs. ExPASy Swiss-Prot
Match: Q10MW6 (DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2)

HSP 1 Score: 560.1 bits (1442), Expect = 3.0e-158
Identity = 304/579 (52.50%), Postives = 402/579 (69.43%), Query Frame = 0

Query: 24  AKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEE 83
           AK +DPYKVLGV+++ASQR+IQKAFHKLSL+YHPDKNK KGAQ+KF EINNAY+ILSDEE
Sbjct: 25  AKNLDPYKVLGVDKSASQRDIQKAFHKLSLKYHPDKNKSKGAQEKFAEINNAYDILSDEE 84

Query: 84  KRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQSQQPFGPGQWQSTGGQGGSQS 143
           KR  +D+YGDEKG+PGFG G+ G++  GYT+FT GG + S    G G WQ+ GGQG +++
Sbjct: 85  KRKNYDLYGDEKGNPGFGGGNFGNR-EGYTYFTGGGAKTSHFSSGDG-WQTMGGQGNTKT 144

Query: 144 FSFSFG----GPSGSNPFGFGMEDIFSNFFGGGFKNEGG---FGSHSKAQSGSRT----S 203
           FSFSFG    G  G NPF F   D+FSN F GG  + GG    GS  KA+ G+++    S
Sbjct: 145 FSFSFGGGNPGAGGGNPFNFDFGDVFSNIFSGG--SMGGSQHTGSAGKARRGTKSSGHDS 204

Query: 204 SSTKV--INKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSLQGALKVGRID 263
           SS  +  +  Q+F KE  DQG+TWLL   T   KG   ++S++E+VA SL GAL+ G+++
Sbjct: 205 SSVNIQEVTMQIFNKETADQGITWLLLFYTPNTKGQFVLESVVEDVARSLDGALRAGKVN 264

Query: 264 CDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFCQEHFPRFSQR 323
           CD E +LCK  G+   ++ R+F+YSY  + +GSL EYSGD   K+LK+FCQEH PRFS+R
Sbjct: 265 CDHEKALCKKAGVSIGKSARLFIYSYTTTEKGSLHEYSGDYDSKSLKTFCQEHLPRFSKR 324

Query: 324 VNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEVTDVSDPSIKK 383
           V++ QF F S+    LP ++LLS KKDTP +WR +SG+FR    FYDAEV DVS P +K 
Sbjct: 325 VDINQFSFPSNIIPNLPQVLLLSAKKDTPAMWRAVSGMFRSRLIFYDAEVQDVSHPLLKS 384

Query: 384 LGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRKAASRPASKT- 443
           LGV  +PA++G   NGE+ +L+ GI+VKDL+S I +L NLL  FEKKN+K AS  A K  
Sbjct: 385 LGVKNIPALIGRSVNGEEQLLKDGISVKDLRSGIKELKNLLENFEKKNKKLASNQAKKPA 444

Query: 444 -QSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESYQDMNENIS 503
                 EN+IPLLT SNF+ +CGEKT VC++G F+SSKA+  LE +L+            
Sbjct: 445 HTDQPKENKIPLLTASNFEEICGEKTSVCILGIFKSSKAKENLEAVLS------------ 504

Query: 504 RTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSSDKILITYKRRK 563
               +SQKTL R QN   G+   V+YAL+D  KQ +FL+ FD + FKSSDK+L+ YK R+
Sbjct: 505 ---EISQKTLIRGQNYNSGN--AVAYALLDGNKQSAFLSTFDKSAFKSSDKLLLAYKPRR 564

Query: 564 GKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           G++A +  E+T EE E+F+ SVLNGDV  +   +KP+L+
Sbjct: 565 GRYAVYDNEVTMEEAERFVVSVLNGDVQLSAAGRKPVLR 582

BLAST of HG10020425 vs. ExPASy Swiss-Prot
Match: P35514 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 94.4 bits (233), Expect = 4.7e-18
Identity = 64/143 (44.76%), Postives = 85/143 (59.44%), Query Frame = 0

Query: 30  YKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRNFFD 89
           Y+ LGV++NASQ EI+KA+ K+S +YHPD NK++GA++K++E+  AYE LSDE+KR  +D
Sbjct: 7   YERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEEKYKEVQEAYETLSDEQKRAAYD 66

Query: 90  MYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQSQQPFGPGQWQSTGGQGGSQSFSFSFG 149
            YG+   + GFG       GGG+                          GG+  FS  FG
Sbjct: 67  QYGEAGANGGFG-------GGGF--------------------------GGASGFS-GFG 112

Query: 150 GPSGSNPFGFGMEDIFSNFFGGG 173
           G  GS  FG G EDIFS+FFGGG
Sbjct: 127 G--GSGGFG-GFEDIFSSFFGGG 112

BLAST of HG10020425 vs. ExPASy Swiss-Prot
Match: Q8EUM4 (Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 4.7e-18
Identity = 63/145 (43.45%), Postives = 82/145 (56.55%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRNF 87
           D Y+VLGV ++A+  +I+ AF K ++QYHPD+NK+  A++KF+E+N AYE+LSD +KR  
Sbjct: 6   DYYEVLGVSKDATDDQIKSAFRKKAMQYHPDRNKEPDAEEKFKEVNQAYEVLSDPDKRAN 65

Query: 88  FDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQSQQPFGPGQWQSTGGQGGSQSFSFS 147
           +D +G E        G  G  GGG  F           PF        GG+GG   F  S
Sbjct: 66  YDRFGHE--------GVDGQFGGGAGF----------DPFDIFNQFFGGGRGGGAHFEQS 124

Query: 148 FGGPSGSNPFGFGMEDIFSNFFGGG 173
           FGG         G EDIFSNFFGGG
Sbjct: 126 FGGS--------GFEDIFSNFFGGG 124

BLAST of HG10020425 vs. ExPASy Swiss-Prot
Match: A2RP20 (Chaperone protein DnaJ OS=Lactococcus lactis subsp. cremoris (strain MG1363) OX=416870 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.1e-18
Identity = 64/143 (44.76%), Postives = 84/143 (58.74%), Query Frame = 0

Query: 30  YKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRNFFD 89
           Y+ LGV++NASQ EI+KA+ K+S +YHPD NK++GA+ K++E+  AYE LSDE+KR  +D
Sbjct: 7   YERLGVDKNASQDEIKKAYRKMSKKYHPDLNKEEGAEDKYKEVQEAYETLSDEQKRAAYD 66

Query: 90  MYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQSQQPFGPGQWQSTGGQGGSQSFSFSFG 149
            YG+   + GFG       GGG+                          GG+  FS  FG
Sbjct: 67  QYGEAGANGGFG-------GGGF--------------------------GGASGFS-GFG 112

Query: 150 GPSGSNPFGFGMEDIFSNFFGGG 173
           G SG   FG G EDIFS+FFGGG
Sbjct: 127 GASGG--FG-GFEDIFSSFFGGG 112

BLAST of HG10020425 vs. ExPASy TrEMBL
Match: A0A5D3DMX5 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00600 PE=4 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.3e-289
Identity = 522/587 (88.93%), Postives = 539/587 (91.82%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLASII ++A LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFG G SGDQGGGYTFFT+GGG
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK++G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINKQ+ KKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVAS+L
Sbjct: 181 GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVASTL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS EGSLVEYSGD+ VK+LK FC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN GRLPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           TD SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRP SKTQSDS ENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLE IL M   
Sbjct: 421 AASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSSDKI 540
                       VSQKTLSRRQN  PGSKET+SYALVDATKQ SFLNAFDSAGFKSSDKI
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKI 540

Query: 541 LITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           LI YKRRKGKFAA+ GEITEEEVEKFIGSVLNGDVTFT TRQKPILK
Sbjct: 541 LIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of HG10020425 vs. ExPASy TrEMBL
Match: A0A5A7TMT4 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005460 PE=4 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 3.2e-288
Identity = 522/588 (88.78%), Postives = 539/588 (91.67%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLASII ++A LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFG G SGDQGGGYTFFT+GGG
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK++G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINKQ+ KKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVAS+L
Sbjct: 181 GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVASTL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS EGSLVEYSGD+ VK+LK FC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN GRLPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           TD SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRP SKTQSDS ENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLE IL M   
Sbjct: 421 AASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAF-DSAGFKSSDK 540
                       VSQKTLSRRQN  PGSKET+SYALVDATKQ SFLNAF DSAGFKSSDK
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDK 540

Query: 541 ILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           ILI YKRRKGKFAA+ GEITEEEVEKFIGSVLNGDVTFT TRQKPILK
Sbjct: 541 ILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of HG10020425 vs. ExPASy TrEMBL
Match: A0A1S3B542 (dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1)

HSP 1 Score: 1000.3 bits (2585), Expect = 3.2e-288
Identity = 522/588 (88.78%), Postives = 539/588 (91.67%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLASII ++A LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFG G SGDQGGGYTFFT+GGG
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFK++G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKDKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINKQ+ KKEIVDQGMTWLLFPTTS LKGVDHVQSIIEEVAS+L
Sbjct: 181 GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVASTL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS EGSLVEYSGD+ VK+LK FC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSNEGSLVEYSGDIDVKSLKGFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN GRLPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           TD SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRP SKTQSDS ENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLE IL M   
Sbjct: 421 AASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILKM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAF-DSAGFKSSDK 540
                       VSQKTLSRRQN  PGSKET+SYALVDATKQ SFLNAF DSAGFKSSDK
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDK 540

Query: 541 ILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           ILI YKRRKGKFAA+ GEITEEEVEKFIGSVLNGDVTFT TRQKPILK
Sbjct: 541 ILIAYKRRKGKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of HG10020425 vs. ExPASy TrEMBL
Match: A0A0A0LDD2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G730990 PE=4 SV=1)

HSP 1 Score: 996.1 bits (2574), Expect = 6.1e-287
Identity = 518/587 (88.25%), Postives = 537/587 (91.48%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M+TRLASIIF+MA LF F LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLASIIFVMAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
            KKGAQQKFEEINNAYEILSDEEKR  FDMYGDEKG+PGFGPGSSGDQGGGYTFFT+GGG
Sbjct: 61  NKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGPGSSGDQGGGYTFFTNGGG 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNEGGFG 180
           RQ QQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFG EDIFSN FGGGF+ +G FG
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGAEDIFSNLFGGGFQGKGSFG 180

Query: 181 SHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASSL 240
            HSKAQSG ++SSS KVINK+  KKEIVDQGMTWLLFPT S LKG+DHVQS IEEVA+SL
Sbjct: 181 GHSKAQSGPKSSSSIKVINKKSLKKEIVDQGMTWLLFPTASSLKGLDHVQSTIEEVANSL 240

Query: 241 QGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSFC 300
           QGALKVGRIDCDSESS CKDLGIYPHRTPRIFVYSYIKS+EGSLVEYSGD+AVK+LKSFC
Sbjct: 241 QGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKSSEGSLVEYSGDIAVKSLKSFC 300

Query: 301 QEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAEV 360
           QEHFPRFSQRVNLKQFDFSSSN G LPTLMLLSTKK+TPVIWRVLSGLFRK F+FYDAEV
Sbjct: 301 QEHFPRFSQRVNLKQFDFSSSNRGGLPTLMLLSTKKETPVIWRVLSGLFRKQFNFYDAEV 360

Query: 361 TDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420
           T  SDPSIKKLGVDALPAIVGWLSNGE+HVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK
Sbjct: 361 TYASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGFEKKNRK 420

Query: 421 AASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMESY 480
           AASRPAS+TQSDS ENQIPLL GSNFDSVCGEKTPVCVIGAFRSSKARNKLE ILNM   
Sbjct: 421 AASRPASRTQSDSGENQIPLLAGSNFDSVCGEKTPVCVIGAFRSSKARNKLESILNM--- 480

Query: 481 QDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSSDKI 540
                       VSQKTLSRRQN  PGSKETVSYALVDATKQ SFLNAFDSAGFKSSDKI
Sbjct: 481 ------------VSQKTLSRRQNSTPGSKETVSYALVDATKQVSFLNAFDSAGFKSSDKI 540

Query: 541 LITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           LI YKRRKGKFAA+  EITEEEVEKFIGSVLNGDVTFTKT QKPILK
Sbjct: 541 LIAYKRRKGKFAAYLDEITEEEVEKFIGSVLNGDVTFTKTHQKPILK 572

BLAST of HG10020425 vs. ExPASy TrEMBL
Match: A0A6J1F5W9 (dnaJ protein ERDJ3A OS=Cucurbita moschata OX=3662 GN=LOC111442396 PE=4 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.9e-273
Identity = 495/588 (84.18%), Postives = 533/588 (90.65%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M+ RL+  I IMASLF FLLS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGG 120
           KKK AQ KFEEINNAYEILSDEEKR  FD+YGDEKGSPGFGPG SGDQGGG+TFFT+ G 
Sbjct: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120

Query: 121 RQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPS-GSNPFGFGMEDIFSNFFGGGFKNEGGF 180
           RQ QQ F P QWQ TGGQGGSQSFSFSFGGPS GSNPFGFGMEDIFSNFFGGGFK+EG F
Sbjct: 121 RQGQQNFKP-QWQGTGGQGGSQSFSFSFGGPSGGSNPFGFGMEDIFSNFFGGGFKDEGNF 180

Query: 181 GSHSKAQSGSRTSSSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVASS 240
           GSH KAQSGS++SS  KV+NKQVFK+E+V QG+TWLLFP TS LKGVDHVQSIIE+VASS
Sbjct: 181 GSHFKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVASS 240

Query: 241 LQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLKSF 300
           LQGALK GRIDC+SESSLCKDLGIYPHRTPRIFVYSYIKST+GSLVEYSGDVAVK+LKSF
Sbjct: 241 LQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKSTDGSLVEYSGDVAVKSLKSF 300

Query: 301 CQEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYDAE 360
           CQEH PRFS+RV+L+QFDFSS+NGGRLPTLMLLSTKKDTPVIWRVLSG++ K FSFYDAE
Sbjct: 301 CQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFSFYDAE 360

Query: 361 VTDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKKNR 420
           VTDVSDP+IKKLGVDALPAIVGWLSNGE+HVLRTGINVKDL+SAIDDLS L+NGFEKKNR
Sbjct: 361 VTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGFEKKNR 420

Query: 421 KAASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNMES 480
           KAAS+PASKTQSDSA+NQIPLLTG NFDS+CG+ TPVCVIGAFRSSKARNKLE+ILNM  
Sbjct: 421 KAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMILNM-- 480

Query: 481 YQDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFKSSDK 540
                        VSQKTLSRRQ   PGSKE++SY+LVDATKQPSFL AFDSAGFKSSD+
Sbjct: 481 -------------VSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQ 540

Query: 541 ILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           ILI YKRRK KFAAH GE+TEEEVEKFIGSVLNGD+TFTKTRQKP+LK
Sbjct: 541 ILIAYKRRKEKFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of HG10020425 vs. TAIR 10
Match: AT3G08970.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 641.7 bits (1654), Expect = 5.6e-184
Identity = 346/591 (58.54%), Postives = 440/591 (74.45%), Query Frame = 0

Query: 3   TRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKK 62
           TRLA  + ++++L   LL+++AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKNK 
Sbjct: 4   TRLAISVVLVSTL--LLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKNKD 63

Query: 63  KGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQ 122
           KGAQ+KF EINNAYEILSDEEKR  +D+YGDEKG PGF  G  G   GGY++ + GGG  
Sbjct: 64  KGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGGYSYSSSGGGFN 123

Query: 123 SQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNE---GGF 182
                GPG WQ+ GG GGS+SFSFSFGGPS S+ FGFGM+DIFS F GG  K +   GGF
Sbjct: 124 FG---GPGGWQNMGGGGGSKSFSFSFGGPSESS-FGFGMDDIFSMFSGGSSKGKEQFGGF 183

Query: 183 GSHSKAQSGSRTS--SSTKVINKQVFKKEIVDQGMTWLLFPTTSYLKGVDHVQSIIEEVA 242
           GS S A+S S++S  ++ K IN QV+KK++VDQGMTWLL       +G  + +SIIEEVA
Sbjct: 184 GSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIEEVA 243

Query: 243 SSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKSTEGSLVEYSGDVAVKNLK 302
            SLQGALKVGR++C++ESSLCK LGI P R PR+FVYSY  S + +L EY+ ++  K +K
Sbjct: 244 ESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSYTSSGKATLAEYTEELVAKKVK 303

Query: 303 SFCQEHFPRFSQRVNLKQFDFSSSNGGRLPTLMLLSTKKDTPVIWRVLSGLFRKHFSFYD 362
           SFCQEH PRFS++++L  FD S+ +  + P ++LLSTKKDTPVIWRVLSGL+   F FY+
Sbjct: 304 SFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNGRFVFYN 363

Query: 363 AEVTDVSDPSIKKLGVDALPAIVGWLSNGEKHVLRTGINVKDLKSAIDDLSNLLNGFEKK 422
            EV D SDP I+KLGVD  PAIVGWLSNGEK VL+TGI VK+LKSA+ +L  LL G EKK
Sbjct: 364 TEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLLEGLEKK 423

Query: 423 NRKAASRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLELILNM 482
           N+K +S+  +    + +  +IPLL+  NFDS+CGE TPVC+IGAFRSS  + KL+ I++ 
Sbjct: 424 NKKVSSKSQAGQAPNESSEKIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEKLQSIMS- 483

Query: 483 ESYQDMNENISRTLRVSQKTLSRRQNPMPGSKETVSYALVDATKQPSFLNAFDSAGFK-S 542
                         +VSQK+LSRRQ    GS++TVSY+L+DATKQ +FL++ D + FK S
Sbjct: 484 --------------KVSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTS 543

Query: 543 SDKILITYKRRKGKFAAHSGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 588
           SDK+LI YK R+GKFA   G++T EEVEKF+ +VLNGD+ FTKTRQKP +K
Sbjct: 544 SDKLLIAYKPRRGKFATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of HG10020425 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 88.6 bits (218), Expect = 1.8e-17
Identity = 43/78 (55.13%), Postives = 60/78 (76.92%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRNF 87
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK  GA++KF+EI+NAYE+LSD+EK++ 
Sbjct: 86  DYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDDEKKSL 145

Query: 88  FDMYGDE--KGSPGFGPG 104
           +D YG+   KG+ GFG G
Sbjct: 146 YDRYGEAGLKGAAGFGNG 163

BLAST of HG10020425 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 88.2 bits (217), Expect = 2.4e-17
Identity = 47/88 (53.41%), Postives = 60/88 (68.18%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRNF 87
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK  GA+ KF+EI+NAYEILSD+EKR+ 
Sbjct: 85  DFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAEDKFKEISNAYEILSDDEKRSL 144

Query: 88  FDMYGDEKGSPGFGPGSSGDQGGGYTFF 116
           +D YG E G  G G G  GD    +  F
Sbjct: 145 YDRYG-EAGVKGAGMGGMGDYSNPFDLF 171

BLAST of HG10020425 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 75.9 bits (185), Expect = 1.2e-13
Identity = 62/177 (35.03%), Postives = 88/177 (49.72%), Query Frame = 0

Query: 1   MDTRLASIIFIMASLFPFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M  R + +  ++ +L   +  L  K+   Y VL V + AS  +I++A+ KL+L+YHPDKN
Sbjct: 1   MAIRWSELCIVLFALSYAICVLAGKSY--YDVLQVPKGASDEQIKRAYRKLALKYHPDKN 60

Query: 61  K-KKGAQQKFEEINNAYEILSDEEKRNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGG 120
           +  + A +KF EINNAYE+LSDEEKR  ++ YG+E                         
Sbjct: 61  QGNEEATRKFAEINNAYEVLSDEEKREIYNKYGEE------------------------- 120

Query: 121 GRQSQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPFGFGMEDIFSNFFGGGFKNE 177
                   G  Q+ + GG+GG        GG       G  M+DIFS+FFGGG   E
Sbjct: 121 --------GLKQFSANGGRGGG-------GG-------GMNMQDIFSSFFGGGSMEE 128

BLAST of HG10020425 vs. TAIR 10
Match: AT2G20560.1 (DNAJ heat shock family protein )

HSP 1 Score: 68.2 bits (165), Expect = 2.6e-11
Identity = 61/171 (35.67%), Postives = 93/171 (54.39%), Query Frame = 0

Query: 27  MDPYKVLGVERNASQREIQKAFHKLSLQYHPDK--NKKKGAQQKFEEINNAYEILSDEEK 86
           +D YKVL V+R+AS  +++KA+ KL++++HPDK  N KK A+  F++I+ AYE+LSD +K
Sbjct: 3   VDYYKVLQVDRSASDDDLKKAYRKLAMKWHPDKNPNNKKDAEAMFKQISEAYEVLSDPQK 62

Query: 87  RNFFDMYGDEKGSPGFGPGSSGDQGGGYTFFTDGGGRQSQQPFGPGQWQSTGGQGGSQSF 146
           +  +D YG+E    G          GG T+F+ G G  S + F P        +     F
Sbjct: 63  KAVYDQYGEE----GLKGNVPPPDAGGATYFSTGDGPTSFR-FNPRNADDIFAE--FFGF 122

Query: 147 SFSFGGPSGSNPFGFGM--EDIFSNFFGGGFKNEGGFGSHSKAQSGSRTSS 194
           S  FGG  G   F   M  +++F++F  G     GG G  S    G+R ++
Sbjct: 123 SSPFGGGRGGTRFSSSMFGDNMFASFGEG-----GGGGGGSMHHGGARKAA 161