Homology
BLAST of HG10020227 vs. NCBI nr
Match:
XP_038904993.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904994.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904995.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904996.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 621/675 (92.00%), Postives = 641/675 (94.96%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNPLQGS F+IKA AQDPPKLYR+NLSP+FSKYDRY AVLMST ARRWGVVYCKN
Sbjct: 1 MGQLLNPLQGSTFIIKAFAQDPPKLYRKNLSPHFSKYDRYRAVLMSTLARRWGVVYCKNF 60
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VN EDHAP WWNLEA+PHVQTLGEFPREEL KVVMVRFDSTLLLL+EMD RINSVKNAV
Sbjct: 61 VNLEDHAPGWWNLEALPHVQTLGEFPREELPGKVVMVRFDSTLLLLEEMDLRINSVKNAV 120
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+KS AKVILASNWN NST RHPDIKSVADYLSSVLQLKVLPVKCSS SM+STEE
Sbjct: 121 FTIKYLHKSAAKVILASNWNTNSTPRHPDIKSVADYLSSVLQLKVLPVKCSSFSMMSTEE 180
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
CFEKADIL+L+NLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGI+RFCS
Sbjct: 181 CFEKADILILKNLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGISRFCS 240
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQEGL L+KAAETKRQPYVAIIGGGNL+DKSAALHLLASTCSALVFVG+MS
Sbjct: 241 TCLAGFNFQEGLGLLRKAAETKRQPYVAIIGGGNLLDKSAALHLLASTCSALVFVGLMSI 300
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDK+VQIVYPKDFWCKNVD SKKME+FAS+
Sbjct: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKNVQIVYPKDFWCKNVDASKKMELFASN 360
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQST G SKLARKLYDLTQR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSTCGTSKLARKLYDLTQR 420
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
CD+T+VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGK LPGVLALDRAYPYKI+WD
Sbjct: 421 GCDVTVVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKPLPGVLALDRAYPYKIDWD 480
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDTALPLVVDIGSGNGMFLLEMA RRKDLNFLGLEINEKLVKRCLH VYQLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMATRRKDLNFLGLEINEKLVKRCLHSVYQLEMKNGH 540
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVE VALRMKEAFLLYGKGKLA+ QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEVVALRMKEAFLLYGKGKLAILQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
Query: 661 FRLMFSKSTTTKSFN 676
FRLM SKSTTT SFN
Sbjct: 661 FRLMLSKSTTTNSFN 675
BLAST of HG10020227 vs. NCBI nr
Match:
XP_038904997.1 (phosphoglycerate kinase, cytosolic-like isoform X2 [Benincasa hispida])
HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 620/675 (91.85%), Postives = 640/675 (94.81%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNPLQGS F+IKA AQDPPKLYR+NLSP+FSKYDRY AVLMST ARRWGVVYCKN
Sbjct: 1 MGQLLNPLQGSTFIIKAFAQDPPKLYRKNLSPHFSKYDRYRAVLMSTLARRWGVVYCKNF 60
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VN EDHAP WWNLEA+PHVQTLGEFPREEL KVVMVRFDSTLLLL+EMD RINSVKNAV
Sbjct: 61 VNLEDHAPGWWNLEALPHVQTLGEFPREELPGKVVMVRFDSTLLLLEEMDLRINSVKNAV 120
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+KS AKVILASNWN NST RHPDIKSVADYLSSVLQLKVLPVKCSS SM+STEE
Sbjct: 121 FTIKYLHKSAAKVILASNWNTNSTPRHPDIKSVADYLSSVLQLKVLPVKCSSFSMMSTEE 180
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
CFEKADIL+L+NLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGI+RFCS
Sbjct: 181 CFEKADILILKNLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGISRFCS 240
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQEGL L+KAAETKRQPYVAIIGGGNL+DKSAALHLLASTCSALVFVG+MS
Sbjct: 241 TCLAGFNFQEGLGLLRKAAETKRQPYVAIIGGGNLLDKSAALHLLASTCSALVFVGLMSI 300
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDK+VQIVYPKDFWCKNVD SKKME+FAS+
Sbjct: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKNVQIVYPKDFWCKNVDASKKMELFASN 360
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNS KIIWIGPVKFSDSSQST G SKLARKLYDLTQR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNS-KIIWIGPVKFSDSSQSTCGTSKLARKLYDLTQR 420
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
CD+T+VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGK LPGVLALDRAYPYKI+WD
Sbjct: 421 GCDVTVVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKPLPGVLALDRAYPYKIDWD 480
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDTALPLVVDIGSGNGMFLLEMA RRKDLNFLGLEINEKLVKRCLH VYQLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMATRRKDLNFLGLEINEKLVKRCLHSVYQLEMKNGH 540
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVE VALRMKEAFLLYGKGKLA+ QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEVVALRMKEAFLLYGKGKLAILQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
Query: 661 FRLMFSKSTTTKSFN 676
FRLM SKSTTT SFN
Sbjct: 661 FRLMLSKSTTTNSFN 674
BLAST of HG10020227 vs. NCBI nr
Match:
XP_016899748.1 (PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo] >XP_016899749.1 PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo])
HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 613/675 (90.81%), Postives = 630/675 (93.33%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNPLQG FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 1 MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 60
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 61 VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 120
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 241 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 300
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 360
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS GAS+LARKLYDL QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 420
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 660
Query: 661 FRLMFSKSTTTKSFN 676
FRLM S STT KSFN
Sbjct: 661 FRLMLSNSTTNKSFN 675
BLAST of HG10020227 vs. NCBI nr
Match:
XP_004137833.1 (phosphoglycerate kinase, cytosolic isoform X1 [Cucumis sativus] >KGN58973.1 hypothetical protein Csa_001234 [Cucumis sativus])
HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNP QG +FVIKAVAQDP KLYRQNLSPYFSKYDRYHAVLMST AR WG VYCKNL
Sbjct: 1 MGQLLNPPQGILFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD + NS+KNAV
Sbjct: 61 VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+KSGAKVILASNWN NS AR+ DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQEGLCQLKKAA+TKRQPYVAIIGGGNL++K AALH+LASTCS LVFVGMMSF
Sbjct: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVS RL+NHGVCKEAA++IQFSLDK V+IVYPKDFWC NVDTSKKMEIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS GAS+LARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
DCDIT+VGTTACKA+MQESSTL AYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLCAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDT LPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTTLPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIV +YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVEAVALRM+EAFLLYGKGKL V QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
Query: 661 FRLMFSKSTTTKSFN 676
FRLM SKS TTKSFN
Sbjct: 661 FRLMLSKS-TTKSFN 674
BLAST of HG10020227 vs. NCBI nr
Match:
TYK25141.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa])
HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 602/661 (91.07%), Postives = 619/661 (93.65%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNPLQG FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 29 MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 89 VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS GAS+LARKLYDL QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 568
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 569 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 628
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 629 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 688
Query: 661 F 662
F
Sbjct: 689 F 689
BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match:
Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)
HSP 1 Score: 181.4 bits (459), Expect = 3.4e-44
Identity = 122/397 (30.73%), Postives = 196/397 (49.37%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G+ EL K V VR D + L D + ++ ++ AV TIK+L +GAKVIL+S+
Sbjct: 79 KSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAKVILSSH 138
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPVK-CSSLSMISTEECFEKADILLLENLSAF 199
+ + LS +L ++V+ V+ C + + +LLLEN+ +
Sbjct: 139 LGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLLLENVRFY 198
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
KEE N +F+K L+ D++VND+F +HR AST G+T+F +AGF Q+ L L
Sbjct: 199 KEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 258
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L C L+ G M F +A GLSV S LV
Sbjct: 259 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 318
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
+ A +++ + K V ++ P D +I + IPDGW+ +D+GP S
Sbjct: 319 EDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 378
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
N L +K +IW GP+ + + G +A+KL DL+ + I G + A+
Sbjct: 379 VKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADLSGKGVTTIIGGGDSVAAV 438
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
+ ++ + +GK LPGV+ALD A
Sbjct: 439 EKVGVASVMSHISTGGGASLELLEGKVLPGVIALDEA 475
BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match:
P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)
HSP 1 Score: 178.3 bits (451), Expect = 2.9e-43
Identity = 119/404 (29.46%), Postives = 201/404 (49.75%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G+ +L K V VR D + L D + ++ ++ A+ TIK+L +GAKVIL+S+
Sbjct: 32 KSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAKVILSSH 91
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
+ + LS +L L+V+ C + + +LLLEN+ +
Sbjct: 92 LGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLLLENVRFY 151
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
KEE N +F+K L+ D++VND+F +HR AST G+T+F +AGF Q+ L L
Sbjct: 152 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 211
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L C L+ G M F +A G+SV S LV
Sbjct: 212 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGMSVGSSLVE 271
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKDFWCKN---VDTSKKMEIFASHDIPDGWLPVDLGP 379
A ++ + +K V ++ P D + D K I + IPDGW+ +D+GP
Sbjct: 272 EDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAADADSK--IVPASGIPDGWMGLDIGP 331
Query: 380 TSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACK 439
S + L ++ +IW GP+ + + +G +A+KL +++++ I G +
Sbjct: 332 DSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGTEAIAKKLEEISKKGATTIIGGGDSVA 391
Query: 440 AMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
A+ + + ++ + +GKQLPGVLAL+ A P +
Sbjct: 392 AVEKVGVAEAMSHISTGGGASLELLEGKQLPGVLALNEADPVPV 433
BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match:
Q9LD57 (Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1 PE=1 SV=1)
HSP 1 Score: 177.6 bits (449), Expect = 5.0e-43
Identity = 118/402 (29.35%), Postives = 196/402 (48.76%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G+ +L K V VR D + L D ++ ++ A+ TIKYL ++GAKVIL+++
Sbjct: 80 KSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILSTH 139
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
+ + LS +L ++V C + S + +LLLEN+ +
Sbjct: 140 LGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFY 199
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
KEE N +F+K L+ D++VND+F +HR AST G+T+F +AGF Q+ L L
Sbjct: 200 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 259
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L C L+ G M F +A GLSV S LV
Sbjct: 260 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 319
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
+ A +++ + K V ++ P D +I + I DGW+ +D+GP S
Sbjct: 320 EDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDS 379
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
N L ++ +IW GP+ + + +G +A KL +L+++ I G + A+
Sbjct: 380 IKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAAV 439
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
+ ++ + +GK LPGV+ALD A P +
Sbjct: 440 EKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPVTV 481
BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match:
Q9SBN4 (Phosphoglycerate kinase, chloroplastic OS=Volvox carteri OX=3067 GN=PGK PE=2 SV=1)
HSP 1 Score: 177.6 bits (449), Expect = 5.0e-43
Identity = 125/404 (30.94%), Postives = 195/404 (48.27%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFD-------STLLLLDEMDARINSVKNAVFTIKYLYKSGAK 139
+++G+ + +L K V VR D TL + D D RI + AV T+KYL +GAK
Sbjct: 64 KSVGDLGKADLEGKRVFVRADLNVPLDKKTLAITD--DTRIRA---AVPTLKYLLDNGAK 123
Query: 140 VILASNWNINSTARHPDIK----SVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADI 199
V+L S ++ P+ K V LS +L +V V C S+ + ++
Sbjct: 124 VLLTS--HLGRPKGGPEDKYRLTPVVARLSELLGKEVKKVDDCIGPSVEQAVASLKSGEL 183
Query: 200 LLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFN 259
LLLEN+ +KEE N +F+K L+ D++VND+F +HR AST G+T+F +AGF
Sbjct: 184 LLLENVRFYKEEEKNDPEFAKKLASNADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFL 243
Query: 260 FQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALG 319
Q+ L L A ++P+VAI+GG + K + L C ++ G M F +A G
Sbjct: 244 LQKELDYLDGAVSAPKRPFVAIVGGSKVSSKITVIEKLMEKCDKIIIGGGMIFTFYKARG 303
Query: 320 LSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGW 379
L V S LV + A ++ + K VQ++ P D D + + + IPDGW
Sbjct: 304 LKVGSSLVEEDKLELAKNLEAIAKAKGVQLLLPSDVVVADKFDANANTQTVSVEAIPDGW 363
Query: 380 LPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITI 439
+ +D+GP S L ++K ++W GP+ + + G +A L +LT + I
Sbjct: 364 MGLDIGPDSIKTFQDALADAKTVVWNGPMGVFEFPKFAVGTVAIANTLSELTPKGAITII 423
Query: 440 VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALD 471
G + A+ Q ++ + +GK LPGV ALD
Sbjct: 424 GGGDSVAAVEQAGVAEKMSHISTGGGASLELLEGKVLPGVAALD 460
BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match:
P41758 (Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 PE=1 SV=1)
HSP 1 Score: 175.3 bits (443), Expect = 2.5e-42
Identity = 125/404 (30.94%), Postives = 191/404 (47.28%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFD-------STLLLLDEMDARINSVKNAVFTIKYLYKSGAK 139
+++G+ + +L K V VR D +TL + D D RI + AV T+KYL +GAK
Sbjct: 63 KSVGDLHKADLEGKRVFVRADLNVPLDKATLAITD--DTRIRA---AVPTLKYLLDNGAK 122
Query: 140 VILASNWNINSTARHPDIK----SVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADI 199
V+L S ++ P+ K V LS +L V V C + + ++
Sbjct: 123 VLLTS--HLGRPKGGPEDKYRLTPVVARLSELLGKPVTKVDDCIGPEVEKAVGAMKNGEL 182
Query: 200 LLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFN 259
LLLEN +KEE N +F+K L+ D++VND+F +HR AST G+T+F +AGF
Sbjct: 183 LLLENCRFYKEEEKNEPEFAKKLAANADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFL 242
Query: 260 FQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALG 319
Q+ L L A ++P+VAI+GG + K + L C ++ G M F +A
Sbjct: 243 LQKELDYLDGAVSNPKRPFVAIVGGSKVSSKITVIEALMEKCDKIIIGGGMIFTFYKARA 302
Query: 320 LSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGW 379
L V S LV + A + + + K VQ++ P D D + + IPDGW
Sbjct: 303 LKVGSSLVEDDKIELAKKLEEMAKAKGVQLLLPTDVVVADKFDANANTQTVPITAIPDGW 362
Query: 380 LPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITI 439
+ +D+GP S N L ++K ++W GP+ + Q +A L LT + C I
Sbjct: 363 MGLDIGPDSVKTFNDALADAKTVVWNGPMGVFEFPQVRQRTVSIANTLAGLTPKGCITII 422
Query: 440 VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALD 471
G + A+ Q ++ + +GK LPGV ALD
Sbjct: 423 GGGDSVAAVEQAGVAEKMSHISTGGGASLELLEGKVLPGVAALD 459
BLAST of HG10020227 vs. ExPASy TrEMBL
Match:
A0A1S4DUU0 (Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103487132 PE=3 SV=1)
HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 613/675 (90.81%), Postives = 630/675 (93.33%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNPLQG FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 1 MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 60
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 61 VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 120
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 241 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 300
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 360
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS GAS+LARKLYDL QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 420
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 660
Query: 661 FRLMFSKSTTTKSFN 676
FRLM S STT KSFN
Sbjct: 661 FRLMLSNSTTNKSFN 675
BLAST of HG10020227 vs. ExPASy TrEMBL
Match:
A0A0A0LB01 (Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G740160 PE=3 SV=1)
HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNP QG +FVIKAVAQDP KLYRQNLSPYFSKYDRYHAVLMST AR WG VYCKNL
Sbjct: 1 MGQLLNPPQGILFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD + NS+KNAV
Sbjct: 61 VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+KSGAKVILASNWN NS AR+ DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQEGLCQLKKAA+TKRQPYVAIIGGGNL++K AALH+LASTCS LVFVGMMSF
Sbjct: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVS RL+NHGVCKEAA++IQFSLDK V+IVYPKDFWC NVDTSKKMEIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS GAS+LARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
DCDIT+VGTTACKA+MQESSTL AYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLCAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDT LPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTTLPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIV +YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVEAVALRM+EAFLLYGKGKL V QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660
Query: 661 FRLMFSKSTTTKSFN 676
FRLM SKS TTKSFN
Sbjct: 661 FRLMLSKS-TTKSFN 674
BLAST of HG10020227 vs. ExPASy TrEMBL
Match:
A0A5D3DNE9 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002660 PE=3 SV=1)
HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 602/661 (91.07%), Postives = 619/661 (93.65%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
MGQLLNPLQG FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 29 MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88
Query: 61 VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 89 VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148
Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208
Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268
Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328
Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388
Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS GAS+LARKLYDL QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448
Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508
Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 568
Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 569 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 628
Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 629 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 688
Query: 661 F 662
F
Sbjct: 689 F 689
BLAST of HG10020227 vs. ExPASy TrEMBL
Match:
A0A6J1J0L1 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111481591 PE=3 SV=1)
HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 592/671 (88.23%), Postives = 628/671 (93.59%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYC-KN 60
MGQLLNP +GSIFV KAVAQD PKLY QNL PYF+KYDRYHAVL ST+ARRWGVVYC KN
Sbjct: 1 MGQLLNPFKGSIFVSKAVAQDSPKLYYQNLFPYFTKYDRYHAVLASTEARRWGVVYCKKN 60
Query: 61 LVNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNA 120
VNSEDHAPSWWNLEAVPHVQTL EFPREELA KVV VRFDSTLLL++E+D RIN VKNA
Sbjct: 61 TVNSEDHAPSWWNLEAVPHVQTLREFPREELAGKVVTVRFDSTLLLMEEIDLRINLVKNA 120
Query: 121 VFTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTE 180
+FT+KYL+KSGAKVILASNWN NS RHP+++SVADYL+SVLQLKVLPV+CSS+SM+ST+
Sbjct: 121 IFTVKYLHKSGAKVILASNWNTNSITRHPNMESVADYLTSVLQLKVLPVQCSSISMMSTK 180
Query: 181 ECFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFC 240
ECFEK DILLL+NLS FKEEVANCSKFSK+LSLGVDIFVNDSFSQSHRILASTVGITRFC
Sbjct: 181 ECFEKVDILLLQNLSEFKEEVANCSKFSKLLSLGVDIFVNDSFSQSHRILASTVGITRFC 240
Query: 241 STCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMS 300
TC+AGF+FQE LCQLK+AA+TKRQPYVAIIGGGNL+DK +ALHLLASTCSALVFVGMMS
Sbjct: 241 CTCIAGFSFQESLCQLKEAAQTKRQPYVAIIGGGNLLDKVSALHLLASTCSALVFVGMMS 300
Query: 301 FQIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360
FQIM ALGL VSSRLVNH VCKEAAD+ QFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS
Sbjct: 301 FQIMHALGLPVSSRLVNHSVCKEAADVFQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360
Query: 361 HDIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQ 420
HDIPDGWLPVDLGPTS DEINALLM SKKIIWIGPVKF +SSQST GAS LARKLYDL Q
Sbjct: 361 HDIPDGWLPVDLGPTSLDEINALLMKSKKIIWIGPVKFFNSSQSTGGASILARKLYDLIQ 420
Query: 421 RDCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINW 480
DCD+T+VGTTACK++MQESSTL A NVFENASVVWDF KGKQLPGVLALDRAYPYKINW
Sbjct: 421 SDCDVTVVGTTACKSLMQESSTLPACNVFENASVVWDFLKGKQLPGVLALDRAYPYKINW 480
Query: 481 DAVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNG 540
DAVFCD ALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLH V QLEM+NG
Sbjct: 481 DAVFCDIALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHSVCQLEMRNG 540
Query: 541 HFIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNG 600
HFIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLA+NG
Sbjct: 541 HFIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLAANG 600
Query: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDP 660
KVFLQSDVEAVALRMKEAFLLYGKGKL+VFQ+QVGEGWLSENPF VRSDWER VLDRGDP
Sbjct: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLSVFQDQVGEGWLSENPFGVRSDWERHVLDRGDP 660
Query: 661 MFRLMFSKSTT 671
MFR+M S STT
Sbjct: 661 MFRIMLSNSTT 671
BLAST of HG10020227 vs. ExPASy TrEMBL
Match:
A0A6J1F8J4 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111441838 PE=3 SV=1)
HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 593/671 (88.38%), Postives = 625/671 (93.14%), Query Frame = 0
Query: 1 MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYC-KN 60
MGQLLNPLQGSIFV KAVAQD PKLY QNL PYF+KYDRYHAVL+ST+ARRWGVVYC KN
Sbjct: 1 MGQLLNPLQGSIFVSKAVAQDSPKLYYQNLFPYFTKYDRYHAVLVSTEARRWGVVYCKKN 60
Query: 61 LVNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNA 120
VNSEDHAPSWWNLEAVPHVQTL EFPREELA KVVMVRFDSTLLL++E+D RIN VKNA
Sbjct: 61 TVNSEDHAPSWWNLEAVPHVQTLREFPREELAGKVVMVRFDSTLLLMEEIDLRINLVKNA 120
Query: 121 VFTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTE 180
VFT+KYL+KSGAKVILASNWN NS RHPD++SVADYL+SVLQLK+LPV+CSS+SM+S +
Sbjct: 121 VFTVKYLHKSGAKVILASNWNTNSITRHPDMESVADYLTSVLQLKILPVQCSSISMMSKK 180
Query: 181 ECFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFC 240
ECFEK DILLLENLS FKEEVANCSKFSK+LSL VDIFVNDSFSQSHRILASTVGITRFC
Sbjct: 181 ECFEKVDILLLENLSEFKEEVANCSKFSKLLSLVVDIFVNDSFSQSHRILASTVGITRFC 240
Query: 241 STCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMS 300
TC+AGF+FQE LCQLK+AA+T RQ YVAIIGGGNL+DK ALH LASTCSALVFVGMMS
Sbjct: 241 CTCIAGFSFQESLCQLKEAAQTTRQTYVAIIGGGNLLDKVTALHFLASTCSALVFVGMMS 300
Query: 301 FQIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360
FQIM ALGL VSSRLVNH VCKEAADIIQFSL+KSVQIVYPKDFWCKNVDTSKKMEIFAS
Sbjct: 301 FQIMHALGLPVSSRLVNHSVCKEAADIIQFSLNKSVQIVYPKDFWCKNVDTSKKMEIFAS 360
Query: 361 HDIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQ 420
HD+PDGWLPVDLGPTS DEINALLM SKKIIWIGPVKF +SSQST GAS LARKLYDL Q
Sbjct: 361 HDVPDGWLPVDLGPTSLDEINALLMKSKKIIWIGPVKFFNSSQSTGGASILARKLYDLIQ 420
Query: 421 RDCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINW 480
DCD+T+VGTTACKA+MQESSTL A NVFENASVVWDF KGK LPGVLALDRAYPYKINW
Sbjct: 421 SDCDVTVVGTTACKALMQESSTLPACNVFENASVVWDFLKGKHLPGVLALDRAYPYKINW 480
Query: 481 DAVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNG 540
DA FCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLH V QLEM+NG
Sbjct: 481 DAAFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHSVCQLEMRNG 540
Query: 541 HFIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNG 600
HFIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLA+NG
Sbjct: 541 HFIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLAANG 600
Query: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDP 660
KVFLQSDVEAVALRMKEAFLLYGKGKL+VFQ+QVGEGWLSENPF VRSDWER VLDRGDP
Sbjct: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLSVFQDQVGEGWLSENPFGVRSDWERHVLDRGDP 660
Query: 661 MFRLMFSKSTT 671
MFRLM S STT
Sbjct: 661 MFRLMLSNSTT 671
BLAST of HG10020227 vs. TAIR 10
Match:
AT3G12780.1 (phosphoglycerate kinase 1 )
HSP 1 Score: 177.6 bits (449), Expect = 3.5e-44
Identity = 118/402 (29.35%), Postives = 196/402 (48.76%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G+ +L K V VR D + L D ++ ++ A+ TIKYL ++GAKVIL+++
Sbjct: 80 KSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILSTH 139
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
+ + LS +L ++V C + S + +LLLEN+ +
Sbjct: 140 LGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFY 199
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
KEE N +F+K L+ D++VND+F +HR AST G+T+F +AGF Q+ L L
Sbjct: 200 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 259
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L C L+ G M F +A GLSV S LV
Sbjct: 260 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 319
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
+ A +++ + K V ++ P D +I + I DGW+ +D+GP S
Sbjct: 320 EDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDS 379
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
N L ++ +IW GP+ + + +G +A KL +L+++ I G + A+
Sbjct: 380 IKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAAV 439
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
+ ++ + +GK LPGV+ALD A P +
Sbjct: 440 EKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPVTV 481
BLAST of HG10020227 vs. TAIR 10
Match:
AT1G56190.1 (Phosphoglycerate kinase family protein )
HSP 1 Score: 172.6 bits (436), Expect = 1.1e-42
Identity = 116/402 (28.86%), Postives = 196/402 (48.76%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G+ +L K V VR D + L D + ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 77 KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 136
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
+ + LS +L ++V+ C + + + +LLLEN+ +
Sbjct: 137 LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 196
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
KEE N F+K L+ D++VND+F +HR AST G+T+F +AGF Q+ L L
Sbjct: 197 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 256
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L C L+ G M F +A GLSV S LV
Sbjct: 257 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 316
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
+ A ++ + + V ++ P D +I + IPDGW+ +D+GP S
Sbjct: 317 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 376
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
N L ++ +IW GP+ + + G +A KL +L+++ I G + A+
Sbjct: 377 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 436
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
+ ++ + +GK LPGV+ALD A P +
Sbjct: 437 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 478
BLAST of HG10020227 vs. TAIR 10
Match:
AT1G56190.2 (Phosphoglycerate kinase family protein )
HSP 1 Score: 172.6 bits (436), Expect = 1.1e-42
Identity = 116/402 (28.86%), Postives = 196/402 (48.76%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G+ +L K V VR D + L D + ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 4 KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 63
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
+ + LS +L ++V+ C + + + +LLLEN+ +
Sbjct: 64 LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 123
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
KEE N F+K L+ D++VND+F +HR AST G+T+F +AGF Q+ L L
Sbjct: 124 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 183
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L C L+ G M F +A GLSV S LV
Sbjct: 184 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 243
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
+ A ++ + + V ++ P D +I + IPDGW+ +D+GP S
Sbjct: 244 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 303
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
N L ++ +IW GP+ + + G +A KL +L+++ I G + A+
Sbjct: 304 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 363
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
+ ++ + +GK LPGV+ALD A P +
Sbjct: 364 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 405
BLAST of HG10020227 vs. TAIR 10
Match:
AT1G79550.1 (phosphoglycerate kinase )
HSP 1 Score: 165.2 bits (417), Expect = 1.8e-40
Identity = 116/397 (29.22%), Postives = 192/397 (48.36%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G +L K V VR D + L D + ++ ++ AV TIKYL +G++V+L S+
Sbjct: 5 RSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCSH 64
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEEC-FEKADILLLENLSAF 199
+K + LS +L ++V+ S + + +LLLEN+ +
Sbjct: 65 LGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFY 124
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
EE N +F+K L+ D++VND+F +HR AST G+ +F +AGF Q+ L L
Sbjct: 125 AEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLV 184
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L +T L+ G M F +A GLSV S LV
Sbjct: 185 GAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLVE 244
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
A +++ + K V ++ P D +I + IPDGW+ +D+GP S
Sbjct: 245 EDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPDS 304
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
+ L +K IIW GP+ + + +G +A++L +L+ + I G + A+
Sbjct: 305 IKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAAV 364
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
+ ++ + +GK LPGVLALD A
Sbjct: 365 EKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401
BLAST of HG10020227 vs. TAIR 10
Match:
AT1G79550.2 (phosphoglycerate kinase )
HSP 1 Score: 165.2 bits (417), Expect = 1.8e-40
Identity = 116/397 (29.22%), Postives = 192/397 (48.36%), Query Frame = 0
Query: 80 QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
+++G +L K V VR D + L D + ++ ++ AV TIKYL +G++V+L S+
Sbjct: 5 RSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCSH 64
Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEEC-FEKADILLLENLSAF 199
+K + LS +L ++V+ S + + +LLLEN+ +
Sbjct: 65 LGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFY 124
Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
EE N +F+K L+ D++VND+F +HR AST G+ +F +AGF Q+ L L
Sbjct: 125 AEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLV 184
Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
A ++P+ AI+GG + K + L +T L+ G M F +A GLSV S LV
Sbjct: 185 GAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLVE 244
Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
A +++ + K V ++ P D +I + IPDGW+ +D+GP S
Sbjct: 245 EDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPDS 304
Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
+ L +K IIW GP+ + + +G +A++L +L+ + I G + A+
Sbjct: 305 IKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAAV 364
Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
+ ++ + +GK LPGVLALD A
Sbjct: 365 EKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_038904993.1 | 0.0e+00 | 92.00 | phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_03890... | [more] |
XP_038904997.1 | 0.0e+00 | 91.85 | phosphoglycerate kinase, cytosolic-like isoform X2 [Benincasa hispida] | [more] |
XP_016899748.1 | 0.0e+00 | 90.81 | PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo] >XP... | [more] |
XP_004137833.1 | 0.0e+00 | 90.67 | phosphoglycerate kinase, cytosolic isoform X1 [Cucumis sativus] >KGN58973.1 hypo... | [more] |
TYK25141.1 | 0.0e+00 | 91.07 | phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa] | [more] |
Match Name | E-value | Identity | Description | |
Q42961 | 3.4e-44 | 30.73 | Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1 | [more] |
P29409 | 2.9e-43 | 29.46 | Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... | [more] |
Q9LD57 | 5.0e-43 | 29.35 | Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1... | [more] |
Q9SBN4 | 5.0e-43 | 30.94 | Phosphoglycerate kinase, chloroplastic OS=Volvox carteri OX=3067 GN=PGK PE=2 SV=... | [more] |
P41758 | 2.5e-42 | 30.94 | Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 PE=1... | [more] |
Match Name | E-value | Identity | Description | |
A0A1S4DUU0 | 0.0e+00 | 90.81 | Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103487132 PE=3 SV=1 | [more] |
A0A0A0LB01 | 0.0e+00 | 90.67 | Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G740160 PE=3 SV=1 | [more] |
A0A5D3DNE9 | 0.0e+00 | 91.07 | Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... | [more] |
A0A6J1J0L1 | 0.0e+00 | 88.23 | Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111481591 PE=3 SV=1 | [more] |
A0A6J1F8J4 | 0.0e+00 | 88.38 | Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111441838 PE=3 SV=1 | [more] |