HG10020227 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10020227
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionPhosphoglycerate kinase
LocationChr04: 30034045 .. 30039320 (+)
RNA-Seq ExpressionHG10020227
SyntenyHG10020227
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCCAACTTTTAAATCCACTTCAAGGATCTATTTTCGTTATTAAAGCAGTTGCTCAAGATCCACCCAAGTTATATCGTCAAAATTTGTCCCCATACTTCTCCAAGTATGACAGATATCATGCTGTCTTAATGTCAACTCAAGGTATAAAGTTAAATTTTCGCTATATATTAAACTTTGAGTTTGTGCATTGATCTTATTCAAGTTTTTGTTTGAAGTTGCATCTCAGCACGAAGGTGGGGGGTAGTCTATTGTAAGAACTTGGTGAATTCAGAGGTATTTAAACGGCTCAGTTTCTGCACCCAATTTATCTTATGATGGAAAGGAAACTTTAGTATATTATTTCTGTGTGCGTATAACTTAAGGTTTCAGTATCCTACTTTCTTTGACCGAGTTTTTAGAACTTGATTTAATTTCTTTGTTTTTTGCCTTATATGAAGTATGCCATTTAAAAGTTTGACGCATGTTCTATGTATTGTATATAATTTGCATGTAGTCTTAATAAAAATCTGGGGTTATTGTAGGACCATGCTCCCAGCTGGTGGAATCTAGAGGCTGTTCCACATGTACAAACTCTTGGGGAGTTTCCTAGGGAAGAACTTGCCCGAAAAGTTGTCATGGTCAGGTTTGATTCGACCTTACTGCTACTGGATGAGATGGACGCAAGGATTAACTCAGTGAAGAATGCAGTCTTCACCATTAAGTACTTATACAAATCTGGAGCAAAAGTGATACTTGCTAGCAACTGGAATATAAACTCTACTGCAAGACATCCTGATATAAAGTCTGTCGCTGGTACACATTCAATGATGTTTCCTGATTTCAATATAGTACACTGTTCTTTTATTTTAAGAATGGAGTTCTATAAGCAACCTCAGATATGGAATATAATGTGTGATGTGGGGTGGACGTGCATTAGAACATGGGGTGATTTCCTCTGGTTCATTGGTTGTGCCCAAATGGACTTGTAAGAGATGATTCAAAGGAGAATTTCAATTTGAGCAGGCTTATAGATGACACTTCCAATTCTATGGTTGGATCATTGAGGATAGATTGGACTCCCTCCTAATAGTATGGATTTGGAGAGAATTTATTATAATTTTGTGTATATATATGTATATATTTTTGGAGATAATTCATTATGTTGAACCTACACGAATATGTCAAAGTAAGATCGAAGTAAATAGAAAACCTTAATGCATGAAAGTTAAACTTTAACAGAGCTCCACTTTTAGACTTCAGATGGTTTTGGTTTGTTAGATTTTACTTAATGCTTAAGAATTGATGGCTTGCAATTTCAGATTATTTGTCCTCTGTTCTTCAACTCAAAGTTCTCCCAGTAAAGTGCAGCTCGTTGAGTATGATTTCAACTGAAGAATGCTTCGAGAAAGCTGATATTCTTCTTCTCGAGAATCTTTCTGCGTTCAAAGAAGAAGTTGCCAATTGCTCCAAATTTTCCAAGATGTTATCTTTAGGTGTTGATATTTTTGTTAATGACTCCTTCTCCCAATCTCATAGAATTCTTGCATCCACGGTTGGGATTACTCGTTTCTGCAGTACCTGCCTAGCTGGTTTTAACTTCCAAGAAGGTTTATGCCAGCTAAAGAAGGCTGCAGAAACCAAGAGGCAACCTTACGTTGCAATTGTATGTTCTCCACTACTAAAATAGATATGAATTTTCCCCTCATCAGCCAAATTACGATTCTATATAATGTATTATGAATTTTTTAACGCAGATTGGAGGAGGCAACCTTGTAGACAAATCGGCTGCCTTGCACCTTTTAGCTTCAACATGCTCTGCGTTGGTCTTTGTTGGAATGATGTCATTCCAGATAATGCAAGCCTTGGGGCTGTCTGTTTCTTCCCGACTGGTGAATCATGGTGTATGTAAGGAAGCTGCAGACATAATCCAATTTTCACTTGATAAAAGCGTACAAATTGTATACCCAAAGGACTTCTGGTGTAAGAATGTAGACACTTCAAAGAAAATGGAAATATTTGCTTCTCATGATATTCCAGATGGTAAGTTTTCATTTTCCCCTACTCGAATGAATATTTATCATTCTTAAGGTTTGACACTTTGAAATTTAAAAGCTTGTTATTTGGTTATCTTAACTTCTTCCTCTTCTAGGTTGGCTACCAGTTGATCTTGGTCCTACATCATCGGATGAAATAAATGCCCTGCTTATGAATAGCAAGGTAGAATTCAAATTTTCCTCAAGTTACAAAAGTTGCTTCTGCTTGATCTAAAATCCCATTTATCTTCCATTGCCTGGTAACTATGAACGATAGTTTTATGATTTAGCACTTCAGGTTGTCCAACTTCTCTTTTATTTAGAAGACAGCAAGTGAATCGCTGTAGCATTTGGCATGCAAAGAAAAACTAGAGTAGATGAAATCATTTCAATTTTTGCTTTATTTGGATATCAATGGAATACTATCTACTTTTGTTGAAATTGTAGGGATTCAATCATTTTTAATGCTCCTACGACTTTTTTGTACCTTTAATGTATATGCCTGTGCAATCAAACTTCAATTCATGGGGATTCAAATCTTTGGGTAGGTGTTGGAGAAGAAGAATTGGCATGTTTTGAATGATGGAATTCTGAAAATATTAAACTCCAGAGAGAATATTACATATCCTAAGATTTAGCTCTTATGGTCATGGACTTACAGTTACAAGAGGTTCATTAATACATCATTCTGCAATTATTTTTCTAAAGCACCTATTAGTAGTTATCTGTTATTTACTCAAGCAAGGATCAGTGTAACACTTTGAGCACTATATTGCAGAAGATTATATGGATTGGACCAGTGAAATTCAGTGATTCAAGCCAAAGTACCAGTGGAGCTTCTAAATTAGCAAGAAAGCTATATGATCTAACCCAAAGGGATTGTGATATAACTATTGTTGGAACCACAGCATGCAAAGCAATGATGCAGGAATCAAGTACCCTATCTGCTTATAATGTTTTTGAGAATGCTTCAGTTGTGTGGGATTTCTTTAAAGGAAAACAACTTCCAGGGGTTTTGGCCTTGGATAGAGTAAGTTTTTTTGGTATTTTCATTATATTTTCTGAAAAACAAAAAAAAAAAAGTTTAAATTGTTACAACAAAAAGAATATAATGTGAATCTATAGGGGAAAATGTCATAAAATCATATTCACACGTCCTGCTTGATGATTGATTTTTTCAATATTACAGGTATTTTCTAGTTTTAGAGGGCTACGGTTTCCATTTAACATCATTACTTTTTCCTGCACTTGTTTAGACTCTTTCATCTACTCGGCAATTCATCCGGTGACTTCACGGTCGCCAAAGAAGCTTTTAAAATGCTATTACTCCATTAAAGACATTCCCACCATAGGATAGATTTGAAGCATGCATCTGAATGAGCAGCTAAAGCTGGTTTGAAGCGATTTGTTCATGCAGGCATACCCATACAAGATTAACTGGGATGCGGTCTTTTGTGATACAGCTCTGCCTTTGGTGGTTGACATTGGAAGTGGTATTATCTTCTTTGTAACATATATTTTGCGGGGCGGGGCGGGCTAAACTTCTTTATATCACTTTTATAGTAAAACTCTGTATTTAACAGCTAAAATGTCAAAGACTTTCATGTGGTGTAGTTTAGCTTAGAATGTGCCGTGAGATAAGAGAAAATAATCTGACATTTTTGGTACAAGTGACTAGTTTTCTTTTTTGTTGGTACAACTGTACAACTTTAAAGAAGCCAATGACGATTGCATATAATTGTTCACTTACGAAGTTAGTTGATAAGTTTTAAGCTGTAAATGACAGGTAATGGGATGTTTCTTCTGGAAATGGCTAGAAGGAGGAAGGATCTTAATTTTCTTGGTTTAGAGATTAATGAAAAGGTCTTCTTTCTTTTCCTTACCCGCATGACCTTAATTATATACATTATCTCACACTTGAATTACCTTTTTCAGCTTGTGAAACGTTGTCTACATTGTGTTTATCAATTAGAGATGAAGAACGGGTAAGGCCTTTATTCATTATTATTATTATTTTGACGTGGCATAGGCTAGACTCAGCAATGTCTGTCAAGACAAATTGTTGGATTAAATAAATTTGAAAAAGTTAATCTTTGGATGATAAGCGAATCTTGGCCTTTCTATGTAGGTGAGCCAAAAAGCATATCAATGAAGTGTCCTCTGTCCCTTATCGCTGAATTAACATGAACCCTTTGCATTGAATCTTTTGTTTCTTTACTATATGTTATCTCAATTTCTCATGATATCAGGCACTTTATTGCGACAAATGCAACGTCGACATTTCATTCTATTGTGTCTAGTTATCCTGGGGAGTTGGTGCTTGTGTCTATACAGGTACAAAACCCTCATGGATCATGTTGTCCCTGATCTTTGGATGTCCCTTCCTAGCCATGATATTTATGCTGAGAACTTATAGAATGTTGTTTGTATTTCTTATTTCGCTATATTTAAGCACTCAATATTTTGCAACTCGGTACTTTGTAGCTAATATCTCCAAATCTGGATCATGCATAGAGTTCACTATCTTCATAACTTAGTGACTTTGAACCACAAACTGATTTTATTCTTAAAAGTTAAGGCCTTAAGGAGCCATTGCCATTTACGAAAGTCTGACTATTTATCGCATTATACAAACCATTCATAGTACTAGCTCTACCTGTATTTATAATTTGTAATTGTACACCTTTGTACCAAAGACTTTGTCTATTTATAACTGAAGGGGGATATTTTGTAAATATAGTGTCCCAACCCCGATTTCAATAAACCAGAGCACAGATGGAGAATGCTGCAAAGGTCATTAGTGGAGGCAGTGGCAGACTTGCTTGCGTCTAATGGCAAGGTACTATCATTTTCTCAGTGCATCTTCACCTCTTTTTTTTTTTCTTTTCAAGTAAATGTTTATCTTGGAAGTTGGACCTCGCTTATGTGACATAATACTACGATCTTTACTCTGGTTATGTAATGATTGGCTTCCTATTTGTTAGGTCTTTCTTCAATCTGATGTTGAAGCTGTTGCTTTGAGAATGAAGGAAGCTTTCTTACTATATGGAAAAGGGAAACTAGCTGTTTTTCAAGAACAAGTTGGGGAAGGATGGCTAAGTGAGAACCCATTTAGAGTAAGATCAGACTGGGAACGACGCGTATTAGATCGTGGGGATCCTATGTTTAGGTTAATGTTCTCAAAATCAACAACAACCAAATCGTTCAACTCACCAGATAGTTTGCCTTTGTTATTGCTATCCACCAATAAAGCTCACTAA

mRNA sequence

ATGGGCCAACTTTTAAATCCACTTCAAGGATCTATTTTCGTTATTAAAGCAGTTGCTCAAGATCCACCCAAGTTATATCGTCAAAATTTGTCCCCATACTTCTCCAAGTATGACAGATATCATGCTGTCTTAATGTCAACTCAAGCACGAAGGTGGGGGGTAGTCTATTGTAAGAACTTGGTGAATTCAGAGGACCATGCTCCCAGCTGGTGGAATCTAGAGGCTGTTCCACATGTACAAACTCTTGGGGAGTTTCCTAGGGAAGAACTTGCCCGAAAAGTTGTCATGGTCAGGTTTGATTCGACCTTACTGCTACTGGATGAGATGGACGCAAGGATTAACTCAGTGAAGAATGCAGTCTTCACCATTAAGTACTTATACAAATCTGGAGCAAAAGTGATACTTGCTAGCAACTGGAATATAAACTCTACTGCAAGACATCCTGATATAAAGTCTGTCGCTGATTATTTGTCCTCTGTTCTTCAACTCAAAGTTCTCCCAGTAAAGTGCAGCTCGTTGAGTATGATTTCAACTGAAGAATGCTTCGAGAAAGCTGATATTCTTCTTCTCGAGAATCTTTCTGCGTTCAAAGAAGAAGTTGCCAATTGCTCCAAATTTTCCAAGATGTTATCTTTAGGTGTTGATATTTTTGTTAATGACTCCTTCTCCCAATCTCATAGAATTCTTGCATCCACGGTTGGGATTACTCGTTTCTGCAGTACCTGCCTAGCTGGTTTTAACTTCCAAGAAGGTTTATGCCAGCTAAAGAAGGCTGCAGAAACCAAGAGGCAACCTTACGTTGCAATTATTGGAGGAGGCAACCTTGTAGACAAATCGGCTGCCTTGCACCTTTTAGCTTCAACATGCTCTGCGTTGGTCTTTGTTGGAATGATGTCATTCCAGATAATGCAAGCCTTGGGGCTGTCTGTTTCTTCCCGACTGGTGAATCATGGTGTATGTAAGGAAGCTGCAGACATAATCCAATTTTCACTTGATAAAAGCGTACAAATTGTATACCCAAAGGACTTCTGGTGTAAGAATGTAGACACTTCAAAGAAAATGGAAATATTTGCTTCTCATGATATTCCAGATGGTTGGCTACCAGTTGATCTTGGTCCTACATCATCGGATGAAATAAATGCCCTGCTTATGAATAGCAAGAAGATTATATGGATTGGACCAGTGAAATTCAGTGATTCAAGCCAAAGTACCAGTGGAGCTTCTAAATTAGCAAGAAAGCTATATGATCTAACCCAAAGGGATTGTGATATAACTATTGTTGGAACCACAGCATGCAAAGCAATGATGCAGGAATCAAGTACCCTATCTGCTTATAATGTTTTTGAGAATGCTTCAGTTGTGTGGGATTTCTTTAAAGGAAAACAACTTCCAGGGGTTTTGGCCTTGGATAGAGCATACCCATACAAGATTAACTGGGATGCGGTCTTTTGTGATACAGCTCTGCCTTTGGTGGTTGACATTGGAAGTGGTAATGGGATGTTTCTTCTGGAAATGGCTAGAAGGAGGAAGGATCTTAATTTTCTTGGTTTAGAGATTAATGAAAAGCTTGTGAAACGTTGTCTACATTGTGTTTATCAATTAGAGATGAAGAACGGGCACTTTATTGCGACAAATGCAACGTCGACATTTCATTCTATTGTGTCTAGTTATCCTGGGGAGTTGGTGCTTGTGTCTATACAGTGTCCCAACCCCGATTTCAATAAACCAGAGCACAGATGGAGAATGCTGCAAAGGTCATTAGTGGAGGCAGTGGCAGACTTGCTTGCGTCTAATGGCAAGGTCTTTCTTCAATCTGATGTTGAAGCTGTTGCTTTGAGAATGAAGGAAGCTTTCTTACTATATGGAAAAGGGAAACTAGCTGTTTTTCAAGAACAAGTTGGGGAAGGATGGCTAAGTGAGAACCCATTTAGAGTAAGATCAGACTGGGAACGACGCGTATTAGATCGTGGGGATCCTATGTTTAGGTTAATGTTCTCAAAATCAACAACAACCAAATCGTTCAACTCACCAGATAGTTTGCCTTTGTTATTGCTATCCACCAATAAAGCTCACTAA

Coding sequence (CDS)

ATGGGCCAACTTTTAAATCCACTTCAAGGATCTATTTTCGTTATTAAAGCAGTTGCTCAAGATCCACCCAAGTTATATCGTCAAAATTTGTCCCCATACTTCTCCAAGTATGACAGATATCATGCTGTCTTAATGTCAACTCAAGCACGAAGGTGGGGGGTAGTCTATTGTAAGAACTTGGTGAATTCAGAGGACCATGCTCCCAGCTGGTGGAATCTAGAGGCTGTTCCACATGTACAAACTCTTGGGGAGTTTCCTAGGGAAGAACTTGCCCGAAAAGTTGTCATGGTCAGGTTTGATTCGACCTTACTGCTACTGGATGAGATGGACGCAAGGATTAACTCAGTGAAGAATGCAGTCTTCACCATTAAGTACTTATACAAATCTGGAGCAAAAGTGATACTTGCTAGCAACTGGAATATAAACTCTACTGCAAGACATCCTGATATAAAGTCTGTCGCTGATTATTTGTCCTCTGTTCTTCAACTCAAAGTTCTCCCAGTAAAGTGCAGCTCGTTGAGTATGATTTCAACTGAAGAATGCTTCGAGAAAGCTGATATTCTTCTTCTCGAGAATCTTTCTGCGTTCAAAGAAGAAGTTGCCAATTGCTCCAAATTTTCCAAGATGTTATCTTTAGGTGTTGATATTTTTGTTAATGACTCCTTCTCCCAATCTCATAGAATTCTTGCATCCACGGTTGGGATTACTCGTTTCTGCAGTACCTGCCTAGCTGGTTTTAACTTCCAAGAAGGTTTATGCCAGCTAAAGAAGGCTGCAGAAACCAAGAGGCAACCTTACGTTGCAATTATTGGAGGAGGCAACCTTGTAGACAAATCGGCTGCCTTGCACCTTTTAGCTTCAACATGCTCTGCGTTGGTCTTTGTTGGAATGATGTCATTCCAGATAATGCAAGCCTTGGGGCTGTCTGTTTCTTCCCGACTGGTGAATCATGGTGTATGTAAGGAAGCTGCAGACATAATCCAATTTTCACTTGATAAAAGCGTACAAATTGTATACCCAAAGGACTTCTGGTGTAAGAATGTAGACACTTCAAAGAAAATGGAAATATTTGCTTCTCATGATATTCCAGATGGTTGGCTACCAGTTGATCTTGGTCCTACATCATCGGATGAAATAAATGCCCTGCTTATGAATAGCAAGAAGATTATATGGATTGGACCAGTGAAATTCAGTGATTCAAGCCAAAGTACCAGTGGAGCTTCTAAATTAGCAAGAAAGCTATATGATCTAACCCAAAGGGATTGTGATATAACTATTGTTGGAACCACAGCATGCAAAGCAATGATGCAGGAATCAAGTACCCTATCTGCTTATAATGTTTTTGAGAATGCTTCAGTTGTGTGGGATTTCTTTAAAGGAAAACAACTTCCAGGGGTTTTGGCCTTGGATAGAGCATACCCATACAAGATTAACTGGGATGCGGTCTTTTGTGATACAGCTCTGCCTTTGGTGGTTGACATTGGAAGTGGTAATGGGATGTTTCTTCTGGAAATGGCTAGAAGGAGGAAGGATCTTAATTTTCTTGGTTTAGAGATTAATGAAAAGCTTGTGAAACGTTGTCTACATTGTGTTTATCAATTAGAGATGAAGAACGGGCACTTTATTGCGACAAATGCAACGTCGACATTTCATTCTATTGTGTCTAGTTATCCTGGGGAGTTGGTGCTTGTGTCTATACAGTGTCCCAACCCCGATTTCAATAAACCAGAGCACAGATGGAGAATGCTGCAAAGGTCATTAGTGGAGGCAGTGGCAGACTTGCTTGCGTCTAATGGCAAGGTCTTTCTTCAATCTGATGTTGAAGCTGTTGCTTTGAGAATGAAGGAAGCTTTCTTACTATATGGAAAAGGGAAACTAGCTGTTTTTCAAGAACAAGTTGGGGAAGGATGGCTAAGTGAGAACCCATTTAGAGTAAGATCAGACTGGGAACGACGCGTATTAGATCGTGGGGATCCTATGTTTAGGTTAATGTTCTCAAAATCAACAACAACCAAATCGTTCAACTCACCAGATAGTTTGCCTTTGTTATTGCTATCCACCAATAAAGCTCACTAA

Protein sequence

MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNLVNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAVFTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEECFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWDAVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGHFIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGKVFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPMFRLMFSKSTTTKSFNSPDSLPLLLLSTNKAH
Homology
BLAST of HG10020227 vs. NCBI nr
Match: XP_038904993.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904994.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904995.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_038904996.1 phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1251.5 bits (3237), Expect = 0.0e+00
Identity = 621/675 (92.00%), Postives = 641/675 (94.96%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNPLQGS F+IKA AQDPPKLYR+NLSP+FSKYDRY AVLMST ARRWGVVYCKN 
Sbjct: 1   MGQLLNPLQGSTFIIKAFAQDPPKLYRKNLSPHFSKYDRYRAVLMSTLARRWGVVYCKNF 60

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VN EDHAP WWNLEA+PHVQTLGEFPREEL  KVVMVRFDSTLLLL+EMD RINSVKNAV
Sbjct: 61  VNLEDHAPGWWNLEALPHVQTLGEFPREELPGKVVMVRFDSTLLLLEEMDLRINSVKNAV 120

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+KS AKVILASNWN NST RHPDIKSVADYLSSVLQLKVLPVKCSS SM+STEE
Sbjct: 121 FTIKYLHKSAAKVILASNWNTNSTPRHPDIKSVADYLSSVLQLKVLPVKCSSFSMMSTEE 180

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           CFEKADIL+L+NLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGI+RFCS
Sbjct: 181 CFEKADILILKNLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGISRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQEGL  L+KAAETKRQPYVAIIGGGNL+DKSAALHLLASTCSALVFVG+MS 
Sbjct: 241 TCLAGFNFQEGLGLLRKAAETKRQPYVAIIGGGNLLDKSAALHLLASTCSALVFVGLMSI 300

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDK+VQIVYPKDFWCKNVD SKKME+FAS+
Sbjct: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKNVQIVYPKDFWCKNVDASKKMELFASN 360

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQST G SKLARKLYDLTQR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSTCGTSKLARKLYDLTQR 420

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
            CD+T+VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGK LPGVLALDRAYPYKI+WD
Sbjct: 421 GCDVTVVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKPLPGVLALDRAYPYKIDWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMA RRKDLNFLGLEINEKLVKRCLH VYQLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMATRRKDLNFLGLEINEKLVKRCLHSVYQLEMKNGH 540

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVE VALRMKEAFLLYGKGKLA+ QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEVVALRMKEAFLLYGKGKLAILQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMFSKSTTTKSFN 676
           FRLM SKSTTT SFN
Sbjct: 661 FRLMLSKSTTTNSFN 675

BLAST of HG10020227 vs. NCBI nr
Match: XP_038904997.1 (phosphoglycerate kinase, cytosolic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1245.0 bits (3220), Expect = 0.0e+00
Identity = 620/675 (91.85%), Postives = 640/675 (94.81%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNPLQGS F+IKA AQDPPKLYR+NLSP+FSKYDRY AVLMST ARRWGVVYCKN 
Sbjct: 1   MGQLLNPLQGSTFIIKAFAQDPPKLYRKNLSPHFSKYDRYRAVLMSTLARRWGVVYCKNF 60

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VN EDHAP WWNLEA+PHVQTLGEFPREEL  KVVMVRFDSTLLLL+EMD RINSVKNAV
Sbjct: 61  VNLEDHAPGWWNLEALPHVQTLGEFPREELPGKVVMVRFDSTLLLLEEMDLRINSVKNAV 120

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+KS AKVILASNWN NST RHPDIKSVADYLSSVLQLKVLPVKCSS SM+STEE
Sbjct: 121 FTIKYLHKSAAKVILASNWNTNSTPRHPDIKSVADYLSSVLQLKVLPVKCSSFSMMSTEE 180

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
           CFEKADIL+L+NLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGI+RFCS
Sbjct: 181 CFEKADILILKNLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGISRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQEGL  L+KAAETKRQPYVAIIGGGNL+DKSAALHLLASTCSALVFVG+MS 
Sbjct: 241 TCLAGFNFQEGLGLLRKAAETKRQPYVAIIGGGNLLDKSAALHLLASTCSALVFVGLMSI 300

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDK+VQIVYPKDFWCKNVD SKKME+FAS+
Sbjct: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKNVQIVYPKDFWCKNVDASKKMELFASN 360

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNS KIIWIGPVKFSDSSQST G SKLARKLYDLTQR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNS-KIIWIGPVKFSDSSQSTCGTSKLARKLYDLTQR 420

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
            CD+T+VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGK LPGVLALDRAYPYKI+WD
Sbjct: 421 GCDVTVVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKPLPGVLALDRAYPYKIDWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMA RRKDLNFLGLEINEKLVKRCLH VYQLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMATRRKDLNFLGLEINEKLVKRCLHSVYQLEMKNGH 540

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVE VALRMKEAFLLYGKGKLA+ QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEVVALRMKEAFLLYGKGKLAILQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMFSKSTTTKSFN 676
           FRLM SKSTTT SFN
Sbjct: 661 FRLMLSKSTTTNSFN 674

BLAST of HG10020227 vs. NCBI nr
Match: XP_016899748.1 (PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo] >XP_016899749.1 PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo])

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 613/675 (90.81%), Postives = 630/675 (93.33%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNPLQG  FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 1   MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 120

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 241 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 300

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 360

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS  GAS+LARKLYDL QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 420

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
           DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 660

Query: 661 FRLMFSKSTTTKSFN 676
           FRLM S STT KSFN
Sbjct: 661 FRLMLSNSTTNKSFN 675

BLAST of HG10020227 vs. NCBI nr
Match: XP_004137833.1 (phosphoglycerate kinase, cytosolic isoform X1 [Cucumis sativus] >KGN58973.1 hypothetical protein Csa_001234 [Cucumis sativus])

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNP QG +FVIKAVAQDP KLYRQNLSPYFSKYDRYHAVLMST AR WG VYCKNL
Sbjct: 1   MGQLLNPPQGILFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD + NS+KNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+KSGAKVILASNWN NS AR+ DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQEGLCQLKKAA+TKRQPYVAIIGGGNL++K AALH+LASTCS LVFVGMMSF
Sbjct: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAA++IQFSLDK V+IVYPKDFWC NVDTSKKMEIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS  GAS+LARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
           DCDIT+VGTTACKA+MQESSTL AYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLCAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDT LPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTTLPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIV +YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVEAVALRM+EAFLLYGKGKL V QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMFSKSTTTKSFN 676
           FRLM SKS TTKSFN
Sbjct: 661 FRLMLSKS-TTKSFN 674

BLAST of HG10020227 vs. NCBI nr
Match: TYK25141.1 (phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 602/661 (91.07%), Postives = 619/661 (93.65%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNPLQG  FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 29  MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 89  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS  GAS+LARKLYDL QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
           DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 568

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 569 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 628

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 629 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 688

Query: 661 F 662
           F
Sbjct: 689 F 689

BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match: Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 3.4e-44
Identity = 122/397 (30.73%), Postives = 196/397 (49.37%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G+    EL  K V VR D  + L D  +   ++ ++ AV TIK+L  +GAKVIL+S+
Sbjct: 79  KSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAKVILSSH 138

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPVK-CSSLSMISTEECFEKADILLLENLSAF 199
                       +  +   LS +L ++V+ V+ C    +        +  +LLLEN+  +
Sbjct: 139 LGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLLLENVRFY 198

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 199 KEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 258

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L   C  L+  G M F   +A GLSV S LV 
Sbjct: 259 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 318

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  +++ +  K V ++ P D             +I  +  IPDGW+ +D+GP S
Sbjct: 319 EDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 378

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               N  L  +K +IW GP+   +  +   G   +A+KL DL+ +     I G  +  A+
Sbjct: 379 VKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADLSGKGVTTIIGGGDSVAAV 438

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
            +        ++        +  +GK LPGV+ALD A
Sbjct: 439 EKVGVASVMSHISTGGGASLELLEGKVLPGVIALDEA 475

BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match: P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 2.9e-43
Identity = 119/404 (29.46%), Postives = 201/404 (49.75%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G+    +L  K V VR D  + L D  +   ++ ++ A+ TIK+L  +GAKVIL+S+
Sbjct: 32  KSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAKVILSSH 91

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
                       +  +   LS +L L+V+    C    +        +  +LLLEN+  +
Sbjct: 92  LGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLLLENVRFY 151

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 152 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 211

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L   C  L+  G M F   +A G+SV S LV 
Sbjct: 212 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGMSVGSSLVE 271

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKDFWCKN---VDTSKKMEIFASHDIPDGWLPVDLGP 379
                 A  ++  + +K V ++ P D    +    D   K  I  +  IPDGW+ +D+GP
Sbjct: 272 EDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAADADSK--IVPASGIPDGWMGLDIGP 331

Query: 380 TSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACK 439
            S    +  L  ++ +IW GP+   +  +  +G   +A+KL +++++     I G  +  
Sbjct: 332 DSIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGTEAIAKKLEEISKKGATTIIGGGDSVA 391

Query: 440 AMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
           A+ +     +  ++        +  +GKQLPGVLAL+ A P  +
Sbjct: 392 AVEKVGVAEAMSHISTGGGASLELLEGKQLPGVLALNEADPVPV 433

BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match: Q9LD57 (Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.0e-43
Identity = 118/402 (29.35%), Postives = 196/402 (48.76%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G+    +L  K V VR D  + L D      ++ ++ A+ TIKYL ++GAKVIL+++
Sbjct: 80  KSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILSTH 139

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
                       +  +   LS +L ++V     C    + S      +  +LLLEN+  +
Sbjct: 140 LGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFY 199

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 200 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 259

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L   C  L+  G M F   +A GLSV S LV 
Sbjct: 260 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 319

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A +++  +  K V ++ P D             +I  +  I DGW+ +D+GP S
Sbjct: 320 EDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDS 379

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               N  L  ++ +IW GP+   +  +  +G   +A KL +L+++     I G  +  A+
Sbjct: 380 IKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAAV 439

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 440 EKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPVTV 481

BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match: Q9SBN4 (Phosphoglycerate kinase, chloroplastic OS=Volvox carteri OX=3067 GN=PGK PE=2 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 5.0e-43
Identity = 125/404 (30.94%), Postives = 195/404 (48.27%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFD-------STLLLLDEMDARINSVKNAVFTIKYLYKSGAK 139
           +++G+  + +L  K V VR D        TL + D  D RI +   AV T+KYL  +GAK
Sbjct: 64  KSVGDLGKADLEGKRVFVRADLNVPLDKKTLAITD--DTRIRA---AVPTLKYLLDNGAK 123

Query: 140 VILASNWNINSTARHPDIK----SVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADI 199
           V+L S  ++      P+ K     V   LS +L  +V  V  C   S+       +  ++
Sbjct: 124 VLLTS--HLGRPKGGPEDKYRLTPVVARLSELLGKEVKKVDDCIGPSVEQAVASLKSGEL 183

Query: 200 LLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFN 259
           LLLEN+  +KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF 
Sbjct: 184 LLLENVRFYKEEEKNDPEFAKKLASNADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFL 243

Query: 260 FQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALG 319
            Q+ L  L  A    ++P+VAI+GG  +  K   +  L   C  ++  G M F   +A G
Sbjct: 244 LQKELDYLDGAVSAPKRPFVAIVGGSKVSSKITVIEKLMEKCDKIIIGGGMIFTFYKARG 303

Query: 320 LSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGW 379
           L V S LV     + A ++   +  K VQ++ P D       D +   +  +   IPDGW
Sbjct: 304 LKVGSSLVEEDKLELAKNLEAIAKAKGVQLLLPSDVVVADKFDANANTQTVSVEAIPDGW 363

Query: 380 LPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITI 439
           + +D+GP S       L ++K ++W GP+   +  +   G   +A  L +LT +     I
Sbjct: 364 MGLDIGPDSIKTFQDALADAKTVVWNGPMGVFEFPKFAVGTVAIANTLSELTPKGAITII 423

Query: 440 VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALD 471
            G  +  A+ Q        ++        +  +GK LPGV ALD
Sbjct: 424 GGGDSVAAVEQAGVAEKMSHISTGGGASLELLEGKVLPGVAALD 460

BLAST of HG10020227 vs. ExPASy Swiss-Prot
Match: P41758 (Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 2.5e-42
Identity = 125/404 (30.94%), Postives = 191/404 (47.28%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFD-------STLLLLDEMDARINSVKNAVFTIKYLYKSGAK 139
           +++G+  + +L  K V VR D       +TL + D  D RI +   AV T+KYL  +GAK
Sbjct: 63  KSVGDLHKADLEGKRVFVRADLNVPLDKATLAITD--DTRIRA---AVPTLKYLLDNGAK 122

Query: 140 VILASNWNINSTARHPDIK----SVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADI 199
           V+L S  ++      P+ K     V   LS +L   V  V  C    +       +  ++
Sbjct: 123 VLLTS--HLGRPKGGPEDKYRLTPVVARLSELLGKPVTKVDDCIGPEVEKAVGAMKNGEL 182

Query: 200 LLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFN 259
           LLLEN   +KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF 
Sbjct: 183 LLLENCRFYKEEEKNEPEFAKKLAANADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFL 242

Query: 260 FQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALG 319
            Q+ L  L  A    ++P+VAI+GG  +  K   +  L   C  ++  G M F   +A  
Sbjct: 243 LQKELDYLDGAVSNPKRPFVAIVGGSKVSSKITVIEALMEKCDKIIIGGGMIFTFYKARA 302

Query: 320 LSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGW 379
           L V S LV     + A  + + +  K VQ++ P D       D +   +      IPDGW
Sbjct: 303 LKVGSSLVEDDKIELAKKLEEMAKAKGVQLLLPTDVVVADKFDANANTQTVPITAIPDGW 362

Query: 380 LPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITI 439
           + +D+GP S    N  L ++K ++W GP+   +  Q       +A  L  LT + C   I
Sbjct: 363 MGLDIGPDSVKTFNDALADAKTVVWNGPMGVFEFPQVRQRTVSIANTLAGLTPKGCITII 422

Query: 440 VGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALD 471
            G  +  A+ Q        ++        +  +GK LPGV ALD
Sbjct: 423 GGGDSVAAVEQAGVAEKMSHISTGGGASLELLEGKVLPGVAALD 459

BLAST of HG10020227 vs. ExPASy TrEMBL
Match: A0A1S4DUU0 (Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103487132 PE=3 SV=1)

HSP 1 Score: 1227.2 bits (3174), Expect = 0.0e+00
Identity = 613/675 (90.81%), Postives = 630/675 (93.33%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNPLQG  FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 1   MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 120

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 241 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 300

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 360

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS  GAS+LARKLYDL QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 420

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
           DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 660

Query: 661 FRLMFSKSTTTKSFN 676
           FRLM S STT KSFN
Sbjct: 661 FRLMLSNSTTNKSFN 675

BLAST of HG10020227 vs. ExPASy TrEMBL
Match: A0A0A0LB01 (Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G740160 PE=3 SV=1)

HSP 1 Score: 1226.1 bits (3171), Expect = 0.0e+00
Identity = 612/675 (90.67%), Postives = 635/675 (94.07%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNP QG +FVIKAVAQDP KLYRQNLSPYFSKYDRYHAVLMST AR WG VYCKNL
Sbjct: 1   MGQLLNPPQGILFVIKAVAQDPAKLYRQNLSPYFSKYDRYHAVLMSTPARSWGAVYCKNL 60

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD + NS+KNAV
Sbjct: 61  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLKTNSMKNAV 120

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+KSGAKVILASNWN NS AR+ DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 121 FTIKYLHKSGAKVILASNWNTNSAARYLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 180

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 181 GFEKADILLLENLSVFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQEGLCQLKKAA+TKRQPYVAIIGGGNL++K AALH+LASTCS LVFVGMMSF
Sbjct: 241 TCLAGFNFQEGLCQLKKAAQTKRQPYVAIIGGGNLLNKLAALHVLASTCSVLVFVGMMSF 300

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAA++IQFSLDK V+IVYPKDFWC NVDTSKKMEIFASH
Sbjct: 301 QIMQALGLSVSYRLMNHGVCKEAAELIQFSLDKRVRIVYPKDFWCANVDTSKKMEIFASH 360

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS  GAS+LARKLYDL+QR
Sbjct: 361 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLSQR 420

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
           DCDIT+VGTTACKA+MQESSTL AYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 421 DCDITVVGTTACKAIMQESSTLCAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 480

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDT LPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 481 AVFCDTTLPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 540

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIV +YPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 541 FIATNATSTFRSIVCNYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVEAVALRM+EAFLLYGKGKL V QEQVGE WLSENPF VRSDWER VLDRGDPM
Sbjct: 601 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQVGEEWLSENPFGVRSDWERHVLDRGDPM 660

Query: 661 FRLMFSKSTTTKSFN 676
           FRLM SKS TTKSFN
Sbjct: 661 FRLMLSKS-TTKSFN 674

BLAST of HG10020227 vs. ExPASy TrEMBL
Match: A0A5D3DNE9 (Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002660 PE=3 SV=1)

HSP 1 Score: 1207.2 bits (3122), Expect = 0.0e+00
Identity = 602/661 (91.07%), Postives = 619/661 (93.65%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYCKNL 60
           MGQLLNPLQG  FVIKAVAQDP KLYRQ LSPYFSKYDRYH VLMST AR WG VYCKNL
Sbjct: 29  MGQLLNPLQGIFFVIKAVAQDPAKLYRQTLSPYFSKYDRYHTVLMSTPARSWGAVYCKNL 88

Query: 61  VNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNAV 120
           VNSEDHA SWWNLEAVPHVQTLGEFPREELA KVVMVRFDSTLLLL+EMD R NS+KNAV
Sbjct: 89  VNSEDHASSWWNLEAVPHVQTLGEFPREELAGKVVMVRFDSTLLLLEEMDLRTNSMKNAV 148

Query: 121 FTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEE 180
           FTIKYL+K GAKVILA+NWN NS ARH DIKSV DYLSSVLQLKV PVKCSS SM+ TEE
Sbjct: 149 FTIKYLHKYGAKVILATNWNTNSAARHLDIKSVVDYLSSVLQLKVCPVKCSSYSMMLTEE 208

Query: 181 CFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 240
            FEKADILLLENLS FKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS
Sbjct: 209 GFEKADILLLENLSMFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCS 268

Query: 241 TCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSF 300
           TCLAGFNFQE LCQLKKAAETKRQPYVAIIGGGNL+DK AALH+LASTCSALVFVGMMSF
Sbjct: 269 TCLAGFNFQERLCQLKKAAETKRQPYVAIIGGGNLLDKLAALHVLASTCSALVFVGMMSF 328

Query: 301 QIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFASH 360
           QIMQALGLSVS RL+NHGVCKEAAD+IQFSLDK VQIVYPKDFWC NVDTSKK+EIFASH
Sbjct: 329 QIMQALGLSVSYRLMNHGVCKEAADLIQFSLDKRVQIVYPKDFWCVNVDTSKKIEIFASH 388

Query: 361 DIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQR 420
           DIPDGWLPVDLGPTS DEINALLMNSKKIIWIGPVKFSDSSQS  GAS+LARKLYDL QR
Sbjct: 389 DIPDGWLPVDLGPTSLDEINALLMNSKKIIWIGPVKFSDSSQSARGASRLARKLYDLAQR 448

Query: 421 DCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINWD 480
           DCDIT+VGTTACKA+MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPY+INWD
Sbjct: 449 DCDITVVGTTACKAIMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYEINWD 508

Query: 481 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNGH 540
           AVFCDTALPLVVDIGSGNGMFLLEMARRRKD NFLGLEINEKLVKRCLH V+QLEMKNGH
Sbjct: 509 AVFCDTALPLVVDIGSGNGMFLLEMARRRKDHNFLGLEINEKLVKRCLHSVHQLEMKNGH 568

Query: 541 FIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 600
           FIATNATSTF SIV SYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK
Sbjct: 569 FIATNATSTFRSIVCSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNGK 628

Query: 601 VFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDPM 660
           VFLQSDVEAVALRM+EAFLLYGKGKL V QEQ GE WLSENPF VRSDWE+ VLDRG PM
Sbjct: 629 VFLQSDVEAVALRMREAFLLYGKGKLGVLQEQFGEEWLSENPFGVRSDWEQHVLDRGHPM 688

Query: 661 F 662
           F
Sbjct: 689 F 689

BLAST of HG10020227 vs. ExPASy TrEMBL
Match: A0A6J1J0L1 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111481591 PE=3 SV=1)

HSP 1 Score: 1200.7 bits (3105), Expect = 0.0e+00
Identity = 592/671 (88.23%), Postives = 628/671 (93.59%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYC-KN 60
           MGQLLNP +GSIFV KAVAQD PKLY QNL PYF+KYDRYHAVL ST+ARRWGVVYC KN
Sbjct: 1   MGQLLNPFKGSIFVSKAVAQDSPKLYYQNLFPYFTKYDRYHAVLASTEARRWGVVYCKKN 60

Query: 61  LVNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNA 120
            VNSEDHAPSWWNLEAVPHVQTL EFPREELA KVV VRFDSTLLL++E+D RIN VKNA
Sbjct: 61  TVNSEDHAPSWWNLEAVPHVQTLREFPREELAGKVVTVRFDSTLLLMEEIDLRINLVKNA 120

Query: 121 VFTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTE 180
           +FT+KYL+KSGAKVILASNWN NS  RHP+++SVADYL+SVLQLKVLPV+CSS+SM+ST+
Sbjct: 121 IFTVKYLHKSGAKVILASNWNTNSITRHPNMESVADYLTSVLQLKVLPVQCSSISMMSTK 180

Query: 181 ECFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFC 240
           ECFEK DILLL+NLS FKEEVANCSKFSK+LSLGVDIFVNDSFSQSHRILASTVGITRFC
Sbjct: 181 ECFEKVDILLLQNLSEFKEEVANCSKFSKLLSLGVDIFVNDSFSQSHRILASTVGITRFC 240

Query: 241 STCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMS 300
            TC+AGF+FQE LCQLK+AA+TKRQPYVAIIGGGNL+DK +ALHLLASTCSALVFVGMMS
Sbjct: 241 CTCIAGFSFQESLCQLKEAAQTKRQPYVAIIGGGNLLDKVSALHLLASTCSALVFVGMMS 300

Query: 301 FQIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360
           FQIM ALGL VSSRLVNH VCKEAAD+ QFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS
Sbjct: 301 FQIMHALGLPVSSRLVNHSVCKEAADVFQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360

Query: 361 HDIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQ 420
           HDIPDGWLPVDLGPTS DEINALLM SKKIIWIGPVKF +SSQST GAS LARKLYDL Q
Sbjct: 361 HDIPDGWLPVDLGPTSLDEINALLMKSKKIIWIGPVKFFNSSQSTGGASILARKLYDLIQ 420

Query: 421 RDCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINW 480
            DCD+T+VGTTACK++MQESSTL A NVFENASVVWDF KGKQLPGVLALDRAYPYKINW
Sbjct: 421 SDCDVTVVGTTACKSLMQESSTLPACNVFENASVVWDFLKGKQLPGVLALDRAYPYKINW 480

Query: 481 DAVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNG 540
           DAVFCD ALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLH V QLEM+NG
Sbjct: 481 DAVFCDIALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHSVCQLEMRNG 540

Query: 541 HFIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNG 600
           HFIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLA+NG
Sbjct: 541 HFIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLAANG 600

Query: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDP 660
           KVFLQSDVEAVALRMKEAFLLYGKGKL+VFQ+QVGEGWLSENPF VRSDWER VLDRGDP
Sbjct: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLSVFQDQVGEGWLSENPFGVRSDWERHVLDRGDP 660

Query: 661 MFRLMFSKSTT 671
           MFR+M S STT
Sbjct: 661 MFRIMLSNSTT 671

BLAST of HG10020227 vs. ExPASy TrEMBL
Match: A0A6J1F8J4 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111441838 PE=3 SV=1)

HSP 1 Score: 1197.2 bits (3096), Expect = 0.0e+00
Identity = 593/671 (88.38%), Postives = 625/671 (93.14%), Query Frame = 0

Query: 1   MGQLLNPLQGSIFVIKAVAQDPPKLYRQNLSPYFSKYDRYHAVLMSTQARRWGVVYC-KN 60
           MGQLLNPLQGSIFV KAVAQD PKLY QNL PYF+KYDRYHAVL+ST+ARRWGVVYC KN
Sbjct: 1   MGQLLNPLQGSIFVSKAVAQDSPKLYYQNLFPYFTKYDRYHAVLVSTEARRWGVVYCKKN 60

Query: 61  LVNSEDHAPSWWNLEAVPHVQTLGEFPREELARKVVMVRFDSTLLLLDEMDARINSVKNA 120
            VNSEDHAPSWWNLEAVPHVQTL EFPREELA KVVMVRFDSTLLL++E+D RIN VKNA
Sbjct: 61  TVNSEDHAPSWWNLEAVPHVQTLREFPREELAGKVVMVRFDSTLLLMEEIDLRINLVKNA 120

Query: 121 VFTIKYLYKSGAKVILASNWNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTE 180
           VFT+KYL+KSGAKVILASNWN NS  RHPD++SVADYL+SVLQLK+LPV+CSS+SM+S +
Sbjct: 121 VFTVKYLHKSGAKVILASNWNTNSITRHPDMESVADYLTSVLQLKILPVQCSSISMMSKK 180

Query: 181 ECFEKADILLLENLSAFKEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFC 240
           ECFEK DILLLENLS FKEEVANCSKFSK+LSL VDIFVNDSFSQSHRILASTVGITRFC
Sbjct: 181 ECFEKVDILLLENLSEFKEEVANCSKFSKLLSLVVDIFVNDSFSQSHRILASTVGITRFC 240

Query: 241 STCLAGFNFQEGLCQLKKAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMS 300
            TC+AGF+FQE LCQLK+AA+T RQ YVAIIGGGNL+DK  ALH LASTCSALVFVGMMS
Sbjct: 241 CTCIAGFSFQESLCQLKEAAQTTRQTYVAIIGGGNLLDKVTALHFLASTCSALVFVGMMS 300

Query: 301 FQIMQALGLSVSSRLVNHGVCKEAADIIQFSLDKSVQIVYPKDFWCKNVDTSKKMEIFAS 360
           FQIM ALGL VSSRLVNH VCKEAADIIQFSL+KSVQIVYPKDFWCKNVDTSKKMEIFAS
Sbjct: 301 FQIMHALGLPVSSRLVNHSVCKEAADIIQFSLNKSVQIVYPKDFWCKNVDTSKKMEIFAS 360

Query: 361 HDIPDGWLPVDLGPTSSDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQ 420
           HD+PDGWLPVDLGPTS DEINALLM SKKIIWIGPVKF +SSQST GAS LARKLYDL Q
Sbjct: 361 HDVPDGWLPVDLGPTSLDEINALLMKSKKIIWIGPVKFFNSSQSTGGASILARKLYDLIQ 420

Query: 421 RDCDITIVGTTACKAMMQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKINW 480
            DCD+T+VGTTACKA+MQESSTL A NVFENASVVWDF KGK LPGVLALDRAYPYKINW
Sbjct: 421 SDCDVTVVGTTACKALMQESSTLPACNVFENASVVWDFLKGKHLPGVLALDRAYPYKINW 480

Query: 481 DAVFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHCVYQLEMKNG 540
           DA FCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLH V QLEM+NG
Sbjct: 481 DAAFCDTALPLVVDIGSGNGMFLLEMARRRKDLNFLGLEINEKLVKRCLHSVCQLEMRNG 540

Query: 541 HFIATNATSTFHSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLASNG 600
           HFIATNATSTF SIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLA+NG
Sbjct: 541 HFIATNATSTFRSIVSSYPGELVLVSIQCPNPDFNKPEHRWRMLQRSLVEAVADLLAANG 600

Query: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLAVFQEQVGEGWLSENPFRVRSDWERRVLDRGDP 660
           KVFLQSDVEAVALRMKEAFLLYGKGKL+VFQ+QVGEGWLSENPF VRSDWER VLDRGDP
Sbjct: 601 KVFLQSDVEAVALRMKEAFLLYGKGKLSVFQDQVGEGWLSENPFGVRSDWERHVLDRGDP 660

Query: 661 MFRLMFSKSTT 671
           MFRLM S STT
Sbjct: 661 MFRLMLSNSTT 671

BLAST of HG10020227 vs. TAIR 10
Match: AT3G12780.1 (phosphoglycerate kinase 1 )

HSP 1 Score: 177.6 bits (449), Expect = 3.5e-44
Identity = 118/402 (29.35%), Postives = 196/402 (48.76%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G+    +L  K V VR D  + L D      ++ ++ A+ TIKYL ++GAKVIL+++
Sbjct: 80  KSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILSTH 139

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
                       +  +   LS +L ++V     C    + S      +  +LLLEN+  +
Sbjct: 140 LGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRFY 199

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N  +F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 200 KEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 259

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L   C  L+  G M F   +A GLSV S LV 
Sbjct: 260 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 319

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A +++  +  K V ++ P D             +I  +  I DGW+ +D+GP S
Sbjct: 320 EDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPDS 379

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               N  L  ++ +IW GP+   +  +  +G   +A KL +L+++     I G  +  A+
Sbjct: 380 IKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAAV 439

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 440 EKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEAIPVTV 481

BLAST of HG10020227 vs. TAIR 10
Match: AT1G56190.1 (Phosphoglycerate kinase family protein )

HSP 1 Score: 172.6 bits (436), Expect = 1.1e-42
Identity = 116/402 (28.86%), Postives = 196/402 (48.76%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G+    +L  K V VR D  + L D  +   ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 77  KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 136

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
                       +  +   LS +L ++V+    C    + +      +  +LLLEN+  +
Sbjct: 137 LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 196

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N   F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 197 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 256

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L   C  L+  G M F   +A GLSV S LV 
Sbjct: 257 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 316

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  ++  +  + V ++ P D             +I  +  IPDGW+ +D+GP S
Sbjct: 317 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 376

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               N  L  ++ +IW GP+   +  +   G   +A KL +L+++     I G  +  A+
Sbjct: 377 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 436

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 437 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 478

BLAST of HG10020227 vs. TAIR 10
Match: AT1G56190.2 (Phosphoglycerate kinase family protein )

HSP 1 Score: 172.6 bits (436), Expect = 1.1e-42
Identity = 116/402 (28.86%), Postives = 196/402 (48.76%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G+    +L  K V VR D  + L D  +   ++ ++ A+ TIK+L ++GAKVIL+++
Sbjct: 4   KSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTH 63

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPV-KCSSLSMISTEECFEKADILLLENLSAF 199
                       +  +   LS +L ++V+    C    + +      +  +LLLEN+  +
Sbjct: 64  LGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRFY 123

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
           KEE  N   F+K L+   D++VND+F  +HR  AST G+T+F    +AGF  Q+ L  L 
Sbjct: 124 KEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYLV 183

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L   C  L+  G M F   +A GLSV S LV 
Sbjct: 184 GAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLVE 243

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
               + A  ++  +  + V ++ P D             +I  +  IPDGW+ +D+GP S
Sbjct: 244 EDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPDS 303

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               N  L  ++ +IW GP+   +  +   G   +A KL +L+++     I G  +  A+
Sbjct: 304 VKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAAV 363

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRAYPYKI 478
            +        ++        +  +GK LPGV+ALD A P  +
Sbjct: 364 EKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEATPVTV 405

BLAST of HG10020227 vs. TAIR 10
Match: AT1G79550.1 (phosphoglycerate kinase )

HSP 1 Score: 165.2 bits (417), Expect = 1.8e-40
Identity = 116/397 (29.22%), Postives = 192/397 (48.36%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G     +L  K V VR D  + L D  +   ++ ++ AV TIKYL  +G++V+L S+
Sbjct: 5   RSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCSH 64

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEEC-FEKADILLLENLSAF 199
                       +K +   LS +L ++V+    S    +        +  +LLLEN+  +
Sbjct: 65  LGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFY 124

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
            EE  N  +F+K L+   D++VND+F  +HR  AST G+ +F    +AGF  Q+ L  L 
Sbjct: 125 AEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLV 184

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L +T   L+  G M F   +A GLSV S LV 
Sbjct: 185 GAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLVE 244

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
                 A  +++ +  K V ++ P D             +I  +  IPDGW+ +D+GP S
Sbjct: 245 EDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPDS 304

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               +  L  +K IIW GP+   +  +  +G   +A++L +L+ +     I G  +  A+
Sbjct: 305 IKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAAV 364

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
            +        ++        +  +GK LPGVLALD A
Sbjct: 365 EKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of HG10020227 vs. TAIR 10
Match: AT1G79550.2 (phosphoglycerate kinase )

HSP 1 Score: 165.2 bits (417), Expect = 1.8e-40
Identity = 116/397 (29.22%), Postives = 192/397 (48.36%), Query Frame = 0

Query: 80  QTLGEFPREELARKVVMVRFDSTLLLLDEMDARINS-VKNAVFTIKYLYKSGAKVILASN 139
           +++G     +L  K V VR D  + L D  +   ++ ++ AV TIKYL  +G++V+L S+
Sbjct: 5   RSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVVLCSH 64

Query: 140 -WNINSTARHPDIKSVADYLSSVLQLKVLPVKCSSLSMISTEEC-FEKADILLLENLSAF 199
                       +K +   LS +L ++V+    S    +        +  +LLLEN+  +
Sbjct: 65  LGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLENVRFY 124

Query: 200 KEEVANCSKFSKMLSLGVDIFVNDSFSQSHRILASTVGITRFCSTCLAGFNFQEGLCQLK 259
            EE  N  +F+K L+   D++VND+F  +HR  AST G+ +F    +AGF  Q+ L  L 
Sbjct: 125 AEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKELDYLV 184

Query: 260 KAAETKRQPYVAIIGGGNLVDKSAALHLLASTCSALVFVGMMSFQIMQALGLSVSSRLVN 319
            A    ++P+ AI+GG  +  K   +  L +T   L+  G M F   +A GLSV S LV 
Sbjct: 185 GAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGSSLVE 244

Query: 320 HGVCKEAADIIQFSLDKSVQIVYPKD-FWCKNVDTSKKMEIFASHDIPDGWLPVDLGPTS 379
                 A  +++ +  K V ++ P D             +I  +  IPDGW+ +D+GP S
Sbjct: 245 EDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDIGPDS 304

Query: 380 SDEINALLMNSKKIIWIGPVKFSDSSQSTSGASKLARKLYDLTQRDCDITIVGTTACKAM 439
               +  L  +K IIW GP+   +  +  +G   +A++L +L+ +     I G  +  A+
Sbjct: 305 IKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDSVAAV 364

Query: 440 MQESSTLSAYNVFENASVVWDFFKGKQLPGVLALDRA 473
            +        ++        +  +GK LPGVLALD A
Sbjct: 365 EKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038904993.10.0e+0092.00phosphoglycerate kinase, cytosolic-like isoform X1 [Benincasa hispida] >XP_03890... [more]
XP_038904997.10.0e+0091.85phosphoglycerate kinase, cytosolic-like isoform X2 [Benincasa hispida][more]
XP_016899748.10.0e+0090.81PREDICTED: phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo] >XP... [more]
XP_004137833.10.0e+0090.67phosphoglycerate kinase, cytosolic isoform X1 [Cucumis sativus] >KGN58973.1 hypo... [more]
TYK25141.10.0e+0091.07phosphoglycerate kinase, cytosolic-like isoform X1 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q429613.4e-4430.73Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
P294092.9e-4329.46Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... [more]
Q9LD575.0e-4329.35Phosphoglycerate kinase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGK1... [more]
Q9SBN45.0e-4330.94Phosphoglycerate kinase, chloroplastic OS=Volvox carteri OX=3067 GN=PGK PE=2 SV=... [more]
P417582.5e-4230.94Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii OX=3055 PE=1... [more]
Match NameE-valueIdentityDescription
A0A1S4DUU00.0e+0090.81Phosphoglycerate kinase OS=Cucumis melo OX=3656 GN=LOC103487132 PE=3 SV=1[more]
A0A0A0LB010.0e+0090.67Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_3G740160 PE=3 SV=1[more]
A0A5D3DNE90.0e+0091.07Phosphoglycerate kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
A0A6J1J0L10.0e+0088.23Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111481591 PE=3 SV=1[more]
A0A6J1F8J40.0e+0088.38Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111441838 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G12780.13.5e-4429.35phosphoglycerate kinase 1 [more]
AT1G56190.11.1e-4228.86Phosphoglycerate kinase family protein [more]
AT1G56190.21.1e-4228.86Phosphoglycerate kinase family protein [more]
AT1G79550.11.8e-4029.22phosphoglycerate kinase [more]
AT1G79550.21.8e-4029.22phosphoglycerate kinase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001576Phosphoglycerate kinasePRINTSPR00477PHGLYCKINASEcoord: 244..266
score: 27.04
coord: 110..132
score: 27.42
coord: 213..235
score: 44.62
coord: 185..200
score: 43.75
coord: 388..413
score: 22.61
coord: 89..105
score: 31.29
coord: 267..286
score: 32.97
coord: 447..464
score: 25.95
IPR001576Phosphoglycerate kinasePFAMPF00162PGKcoord: 90..439
e-value: 2.1E-54
score: 185.0
IPR001576Phosphoglycerate kinasePANTHERPTHR11406PHOSPHOGLYCERATE KINASEcoord: 39..544
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 259..468
e-value: 8.2E-35
score: 122.2
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 78..258
e-value: 1.3E-30
score: 108.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 469..675
e-value: 3.2E-50
score: 172.2
NoneNo IPR availablePIRSRPIRSR000724-2PIRSR000724-2coord: 90..311
e-value: 4.2E-14
score: 49.8
NoneNo IPR availablePANTHERPTHR11406:SF13PHOSPHOGLYCERATE KINASEcoord: 39..544
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 491..604
e-value: 1.68994E-5
score: 42.4171
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePFAMPF02390Methyltransf_4coord: 489..662
e-value: 4.3E-26
score: 91.4
IPR003358tRNA (guanine-N-7) methyltransferase, Trmb typePROSITEPS51625SAM_MT_TRMBcoord: 442..667
score: 43.928841
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 487..665
IPR036043Phosphoglycerate kinase superfamilySUPERFAMILY53748Phosphoglycerate kinasecoord: 89..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10020227.1HG10020227.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
biological_process GO:0106004 tRNA (guanine-N7)-methylation
biological_process GO:0006400 tRNA modification
cellular_component GO:0005829 cytosol
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004618 phosphoglycerate kinase activity
molecular_function GO:0008176 tRNA (guanine-N7-)-methyltransferase activity