HG10019934 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019934
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Target of rapamycin complex subunit LST8
Location	Chr04: 27090309 .. 27094499 (+)
RNA-Seq Expression	HG10019934
Synteny	HG10019934

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCAACCATCGGTGATACTTGCAACCGCCAGCTATGATCATACTATTAGGTTTTGGGAGGCCAAAAGTGGTCGCTGTTACCGAACTATACAATATCCTGATTCTGTTAGTTCATAAATCCTTTCTGTCTGGTGTTTCTCTGTGTTTCTTGGTTATTCTGCATTATGTTTTTCTTTTCTTCTGTTAGAAATTTTGAAAGAAGTTTTGGTCGCATTACAAATTTGATAAACAGGAAAGTTAACTAATTACCATAAATGTGTCCCTTACCCCTTGGTAGTTTGGTTGAGCAGGGTGGTAGTTCTGTTTCAGGGCTTTTCTTTCCCAACTAGAAGTTAGCCTTACCCTTTGGTTTCTTCTCTCAACTGTTAATGATGAATGTTTTTTGCTCCTTCATTTGCCTAATTTCTATGAATAATGTGCACAGCAAGTTAATAGACTGGAGATTACCCCAGACAAAAGATATTTGGCTGCTGCTGGTAATCCTCACATACGGTTGTTTGATATCAATTCAAACAGCCCTCAACCAGTACGTAACCTGCTCATCTCTTAACACCTTACTGTTGTTATTTAATTTTTCTTCAACTATTTTTTTCTTCCAGAATCTCTTTAGATGGAAGCTGAGTGAAAATAAATCTTCCCTTCATTTTCTAATGCGTTTGTTCTTTCTATTTTTACCCCCAACCTATTTGGCAGATAATGAGTTATGATTCACATACTAATAATGTAATGTCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGCACGGTAAAAATATGGGATTTGAGGTAAAGAAAGCTTTCGAATTAACGTGTCAATTGTTGTCACTATTTGTTTATGTACATATGTTTGATTTTGTTTAGACTTACACTCAAGGTTATCTGGACTTATTTTGAGAAATACAGGGCTCCTGGTTGTCAGAGGGAGTATGAAAGTCGTGCTGCAGTTAACACTGTTGTTCTGCATCCTAATCAGGTCATTTATATCATCTCTACCCCTTTTTGCAGTTTGATGTGATTTTACTTCAAATCCTATTAGTCCTACTTAAGTAATGGAAGTTTTTTTCTTTTCACAATGATAATAATAATAATAATAATACTGCGCCAGACAATGCACAAGAGCCTCTGCTGAGTGCAGTTTATTTGTTTTACTTTTTGGGCATGAGAAGGAATATAATTTTACAATCTTCATTTAACCAATTCTGTCTTTCTTTCCTCTGAGAGATTCATCCTAGAAAAAACTACTAACCTCATCATTTTGCTTTTGCATTGTTAACCAAATTTCTCTCGTTTGCAGACTGAACTGATATCTGGAGATCAAAATGGCAATATCCGTGTGTGGGATTTGACTGCAAATTCATGCAGCTGTGAATTGGTATGGCTTTGTGCATTTCATTTGTGTAATCGTAATTGAATGTGCCTCTTCCTTGTTATATTTTGTTTCCAAATGATTGTAGATTGTCGTATTCCTCTTTGTGATGTCAGGCTAACACGCTTTATAAGAGGAAAAATTACTCAAACGAAAATAACATCTTTAGGTGAAAAATATTAGCAACTAGTCTCAACATTATTGCTTAAAGCTTGAGATGAAAATGAAATACCCTCAGGAGGACAATGATATGGAAATGCAAGGACTTGGATAAAATCTGTTGGTATTAGCGCACTTCATCAATCTTTACGTGAAGCTGTGCAAAGAAGGAAGTAGTTGATAACGTAGCATTGGGGTTTCGGATAGTTGTTTAAGTGTTCGTAGTGTAGATGCTTCTAATTTTGAAATGAAATACCCTCAGGAGGACAATGATATGGAAATGCAAGGACTTGGATAAAATCTGTTGGTATTAGCGCACTTCATCACTCTTTACGAGAAGCTGTGCAAAGAAGGAAGTAGTTGATAATGTAGTATTGGGGCTTCGGATAGTTGTTTAAGTGTTCGTAGTGTAGATGCTTCTAACTTTGAACTTGACGAAAATAAGGGAGTTTTTGAGCTTGATACCTCTAATATTTATGGGGAGTTAACTACCGTGTTTCTTTTTAATGATGAGGAAAACAAAGAAATTGATGTGTAGAGGGATGTGGATGCCTGTTGGGCTTGGGATCTTTTGGTCTTACTGGCTTAGAGGTCCTGAGTATGAACCTTCGAGTGAGCTTAATACAAAGCACCCTTGATATCTTCTGAATCTGGGCCTTGGGGCAGGTGCATGTGCCCTTGGGTATAGGGTTTAGGATGGAAGTTCTTTCTGTGGATGAGGAAAAATCATCATCAAAAGGATTGGATAAGGAAAATAAACTTATGAAAGAACAGTGTGGGTACTTAGAGAAGGTCCGGGTTAATTCTTCATTCCTCTCAAGAGTTCAATCTCGAGCAATTGGAAAAATTAGAAGAAAAAGAAGTGAAAGGGACAAGCTGTCCTTAAGACAGTTAAACTATGGAAGTATAAGACACTTCGTTAGGGGTTCTGAAGTTTAGAGATAAAAGAGGTTAAGAAAATCAAAGATTTCAGAAAGCTAGTTCTGTTGTAATACTTTTTCTAGGATGCTACTGTGAGGTGTTTCAAGGTGACTTGGCCCTCTTTGGAAGAATTCTATGTTTAATGGGTCTGTGAATTCCTTCAAGTGTTAAATAGGTCAAGGAAATATTAGAGAAAAAAGTGGAAGTCCAAAGACCTATTAAACACCAAACTGAAAGTTTAAAGATTTACTAGACAAAATTCAGGGACTTTGATACAAATTTGAAAGTTAGGAATCTATTAGATCCTAACTTAGGCTCAAGGGCCTGAACTTTTAATTTAACCTATTTGTAATCCCCTAAATGAGAGCTTTGTACTTTATTTACTGCCAAAAAAAGGAACAAAAGTCGCCTAGTGACATGGATTATCATGGCTACATCCCAATAATCTAGTGTGATCCCAGGTGCCAGAAGTGGATACAGCTGTAAGATCTTTGACGGTTATGTGGGATGGGAGCTTGGTGGTTGCAGCAAATAATCATGGCACATGTTACGTCTGGCGCTTGTTACGTGGCACCCAGGTTGGACCTAAATGCCAAATGCAAATTATTGTCTGCTTTACAATTCAATTAGTGTAACTCATTTTTCTCATTCAGACGATGACAAACTTTGAGCCACTTCATAAACTTCAAGCACACAAGGGATACATTCTCAAGTGTCTTCTTTCACCTGAATTCTGTGAACCCCACCGGTCTTATTCCTCAGAACTATATACATTCTCTTTTCTTTCATTTCTTCTTGTTGGACAATATATTTTTGAATAAATGATTGTTGGAACTCGGATATTTCTGCAAATTATAACATTCACATGGCTTCACATCTTTTAGATACCTTGCCACTGCATCTTCGGACAGCACTGTCAAAATCTGGAACGTGGATGGTTTCACGTTAGAAAAGACACTTATAGGTATATATCTCTCATTTATATAGTGTTATTTCTATAAAAGACATATGGTTATTCTATTCAATTATCATTAGACTGGATCTTACTGGCAGTTACTGAGAAGCAAGTATAATGCTTGGTTTTGCAATTCTATGTGCTTTTCAGGACACCAACGCTGGGTGTGGGACTGCGTCTTCTCCGTAGATGGTGCATATCTTATAACAGGTGACTGGGTTATTGACAAATCTGAGGGCCTCAGTAGGCAATATTTTCTTTATAAATTTCTTTGAAAGCATTGATCTCTGTTGACTATGCATTTGGAGTAAGCTATATACCGAGATATCACTGCAAAACCAAAGTAGCTTTTCTTAGATACAAATTATCCTACAAATTTTTTTCCCCTAACGTCAGTCCCTTAGATGTTATAAGGGTCAGGTTAGTTGTGATATCAATGCGCAAAAAAGAAAAAGGTGTGGATATTCGTAGTTATATAGGAAATAAACTCTAACTACAACAGTTGCAATTAGAATGGAAAAACTTCTGTTGTTTGTTTTAGTTTATTTTTATTTTTGGCATTTTCCAAAAATATTGATGTATCCGTTTAAGCCTTTCCTGTATGTTTGTGAAAATAACTTGGGATGTCTGTGAATGCAGCTTCTTCTGATACTACTGCAAGACTCTGGTCCATGTCAACTGGCAAAGAGATGAAGGTTTATCAAGGTCATCATAAAGCAACTATTTGTTGTGCCCTCCACGATGGAGCCGAGCCATCGTCATGCTGA

mRNA sequence

ATGACTCAACCATCGGTGATACTTGCAACCGCCAGCTATGATCATACTATTAGGTTTTGGGAGGCCAAAAGTGGTCGCTGTTACCGAACTATACAATATCCTGATTCTCAAGTTAATAGACTGGAGATTACCCCAGACAAAAGATATTTGGCTGCTGCTGGTAATCCTCACATACGGTTGTTTGATATCAATTCAAACAGCCCTCAACCAATAATGAGTTATGATTCACATACTAATAATGTAATGTCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGCACGGTAAAAATATGGGATTTGAGACTTACACTCAAGGTTATCTGGACTTATTTTGAGAAATACAGGGCTCCTGGTTGTCAGAGGGAGTATGAAAGTCGTGCTGCAGTTAACACTGTTGTTCTGCATCCTAATCAGACTGAACTGATATCTGGAGATCAAAATGGCAATATCCGTGTGTGGGATTTGACTGCAAATTCATGCAGCTGTGAATTGGTGCATGTGCCCTTGGGTATAGGGTTTAGGATGGAAGTTCTTTCTGTGGATGAGGAAAAATCATCATCAAAAGGATTGGATAAGGAAAATAAACTTATGAAAGAACAGTGTGGATACCTTGCCACTGCATCTTCGGACAGCACTGTCAAAATCTGGAACGTGGATGGTTTCACGTTAGAAAAGACACTTATAGGACACCAACGCTGGGTGTGGGACTGCGTCTTCTCCGTAGATGGTGCATATCTTATAACAGCTTCTTCTGATACTACTGCAAGACTCTGGTCCATGTCAACTGGCAAAGAGATGAAGGTTTATCAAGGTCATCATAAAGCAACTATTTGTTGTGCCCTCCACGATGGAGCCGAGCCATCGTCATGCTGA

Coding sequence (CDS)

ATGACTCAACCATCGGTGATACTTGCAACCGCCAGCTATGATCATACTATTAGGTTTTGGGAGGCCAAAAGTGGTCGCTGTTACCGAACTATACAATATCCTGATTCTCAAGTTAATAGACTGGAGATTACCCCAGACAAAAGATATTTGGCTGCTGCTGGTAATCCTCACATACGGTTGTTTGATATCAATTCAAACAGCCCTCAACCAATAATGAGTTATGATTCACATACTAATAATGTAATGTCAGTGGGGTTTCAATGTGATGGGAACTGGATGTATTCAGGTTCTGAGGATGGCACGGTAAAAATATGGGATTTGAGACTTACACTCAAGGTTATCTGGACTTATTTTGAGAAATACAGGGCTCCTGGTTGTCAGAGGGAGTATGAAAGTCGTGCTGCAGTTAACACTGTTGTTCTGCATCCTAATCAGACTGAACTGATATCTGGAGATCAAAATGGCAATATCCGTGTGTGGGATTTGACTGCAAATTCATGCAGCTGTGAATTGGTGCATGTGCCCTTGGGTATAGGGTTTAGGATGGAAGTTCTTTCTGTGGATGAGGAAAAATCATCATCAAAAGGATTGGATAAGGAAAATAAACTTATGAAAGAACAGTGTGGATACCTTGCCACTGCATCTTCGGACAGCACTGTCAAAATCTGGAACGTGGATGGTTTCACGTTAGAAAAGACACTTATAGGACACCAACGCTGGGTGTGGGACTGCGTCTTCTCCGTAGATGGTGCATATCTTATAACAGCTTCTTCTGATACTACTGCAAGACTCTGGTCCATGTCAACTGGCAAAGAGATGAAGGTTTATCAAGGTCATCATAAAGCAACTATTTGTTGTGCCCTCCACGATGGAGCCGAGCCATCGTCATGCTGA

Protein sequence

MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGFRMEVLSVDEEKSSSKGLDKENKLMKEQCGYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATICCALHDGAEPSSC

Homology

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BLAST of HG10019934 vs. NCBI nr
Match: XP_038906292.1 (target of rapamycin complex subunit LST8-1 [Benincasa hispida] >XP_038906293.1 target of rapamycin complex subunit LST8-1 [Benincasa hispida])

HSP 1 Score: 494.2 bits (1271), Expect = 7.9e-136
Identity = 253/331 (76.44%), Postives = 258/331 (77.95%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 298
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

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BLAST of HG10019934 vs. NCBI nr
Match: XP_004136587.1 (target of rapamycin complex subunit LST8-1 [Cucumis sativus] >XP_008443103.1 PREDICTED: protein LST8 homolog [Cucumis melo] >XP_011652119.1 target of rapamycin complex subunit LST8-1 [Cucumis sativus] >KAA0043702.1 protein LST8-like protein [Cucumis melo var. makuwa] >TYK25432.1 protein LST8-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 491.1 bits (1263), Expect = 6.7e-135
Identity = 252/330 (76.36%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. NCBI nr
Match: XP_023528357.1 (protein LST8 homolog [Cucurbita pepo subsp. pepo] >XP_023528358.1 protein LST8 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 491.1 bits (1263), Expect = 6.7e-135
Identity = 252/330 (76.36%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. NCBI nr
Match: XP_022154284.1 (protein LST8 homolog [Momordica charantia])

HSP 1 Score: 490.0 bits (1260), Expect = 1.5e-134
Identity = 251/330 (76.06%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVN+LEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNKLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. NCBI nr
Match: XP_022935415.1 (protein LST8 homolog isoform X1 [Cucurbita moschata] >XP_022935416.1 protein LST8 homolog isoform X1 [Cucurbita moschata] >XP_022935417.1 protein LST8 homolog isoform X1 [Cucurbita moschata] >KAG7017580.1 Protein LST8-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 488.8 bits (1257), Expect = 3.3e-134
Identity = 250/330 (75.76%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FD+NSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDLNSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. ExPASy Swiss-Prot
Match: Q9LV27 (Target of rapamycin complex subunit LST8-1 OS=Arabidopsis thaliana OX=3702 GN=LST8-1 PE=1 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 1.3e-120
Identity = 217/319 (68.03%), Postives = 235/319 (73.67%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEA++GRCYRTIQYPDS VNRLEITPDK YLAAA NPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAETGRCYRTIQYPDSHVNRLEITPDKHYLAAACNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FD+NSNSPQP+M+YDSHTNNVM+VGFQCD  WMYSGSEDGTVKIWDL             
Sbjct: 61  FDVNSNSPQPVMTYDSHTNNVMAVGFQCDAKWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHV------ 180
            RAPGCQ+EYES AAVNTVVLHPNQTELISGDQNGNIRVWDL ANSCSCELV        
Sbjct: 121 -RAPGCQKEYESVAAVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVPEVDTAVR 180

Query: 181 ---------------PLGIGFRMEVLSVDEEKSSSKGLDKENK--------LMKEQCGYL 240
                            G  +   +L   +  +  + L K           L+     YL
Sbjct: 181 SLTVMWDGTMVVAANNRGTCYVWRLLRGKQTMTEFEPLHKLQAHNGHILKCLLSPANKYL 240

Query: 241 ATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGK 291
           ATASSD TVKIWNVDGF LEK L GHQRWVWDCVFSVDG +L+TASSD TARLWSM  GK
Sbjct: 241 ATASSDKTVKIWNVDGFKLEKVLTGHQRWVWDCVFSVDGEFLVTASSDMTARLWSMPAGK 300

[Close]

BLAST of HG10019934 vs. ExPASy Swiss-Prot
Match: Q9AYE4 (Target of rapamycin complex subunit LST8 OS=Oryza sativa subsp. japonica OX=39947 GN=LST8 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 5.0e-117
Identity = 213/326 (65.34%), Postives = 237/326 (72.70%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M QPSVILATASYDH+I+FWEAKSGRCYRT+Q+ +S +NRLEITPDKR+LAAAGNPHIRL
Sbjct: 1   MAQPSVILATASYDHSIKFWEAKSGRCYRTLQHTESHINRLEITPDKRFLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNS  P++SYDSHT+NVM+VGF CDGNWMYSGSEDGTV+IWDLR            
Sbjct: 61  FDINSNSNHPVISYDSHTSNVMAVGFHCDGNWMYSGSEDGTVRIWDLRTAT--------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHV------ 180
                CQREYESRAAVNTVVLHPNQ ELISGDQNGNIRVWDL ANSCSCELV        
Sbjct: 121 -----CQREYESRAAVNTVVLHPNQKELISGDQNGNIRVWDLAANSCSCELVPEVDTAVR 180

Query: 181 ---------------PLGIGFRMEVLSVDEEKSSSKGLDK---------ENKLMKEQCG- 240
                            G  +   +L   +  +  + L K         +  L  E C  
Sbjct: 181 SLTVMWDGSMVVAANNRGTCYVWRLLKGTQTITCFEPLHKLQAHDGYILKCLLSPEFCDP 240

Query: 241 --YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSM 294
             YLATASSD TVKIWNVDGF LEKTL+GHQRWVWDCVFSVDGAYLITASSDTTARLW+M
Sbjct: 241 NRYLATASSDHTVKIWNVDGFKLEKTLVGHQRWVWDCVFSVDGAYLITASSDTTARLWTM 300

[Close]

BLAST of HG10019934 vs. ExPASy Swiss-Prot
Match: F4IIK6 (Non-functional target of rapamycin complex subunit LST8-2 OS=Arabidopsis thaliana OX=3702 GN=LST8-2 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.5e-89
Identity = 176/315 (55.87%), Postives = 208/315 (66.03%), Query Frame = 0


Query: 6   VILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINS 65
           V LATAS+D TIR W+A++GRCY + +YPD  VNRLE+TP+K  L AA NPHIRLFD+ S
Sbjct: 11  VYLATASHDQTIRLWQARTGRCYFSFRYPDLHVNRLELTPEKGKLVAACNPHIRLFDLRS 70

Query: 66  NSPQ-PIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYRAP 125
            +P  P+ ++ SHT NVM+VGFQ  G+ MYSGSEDG+VKIWDLR+               
Sbjct: 71  YNPHIPVRNFVSHTKNVMAVGFQYTGHMMYSGSEDGSVKIWDLRVR-------------- 130

Query: 126 GCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGFR--- 185
            CQRE+ S + VNTVVLHPNQTELISGDQNGNIRVWDL A+ CSCELV   +G   R   
Sbjct: 131 ECQREFRSVSPVNTVVLHPNQTELISGDQNGNIRVWDLRADLCSCELV-PEVGTPIRSLT 190

Query: 186 ------MEVLSVDE----------EKSSSKGLDKENKLMKE-----QC-------GYLAT 245
                 M V + D           E+ +    +  +KL        +C        YLAT
Sbjct: 191 VMWDGTMVVAANDRGTCYVWRSLCERQTMTEFEPLHKLQAHNSHILKCLLSPGNNRYLAT 250

Query: 246 ASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEM 289
           ASSD TVKIWN+DGF LEK L GH+RWVWDC FS+DG YL+TASSDTTARLWSM  GKE 
Sbjct: 251 ASSDKTVKIWNLDGFKLEKVLTGHERWVWDCDFSMDGEYLVTASSDTTARLWSMRAGKEE 310

[Close]

BLAST of HG10019934 vs. ExPASy Swiss-Prot
Match: Q54D08 (Protein LST8 homolog OS=Dictyostelium discoideum OX=44689 GN=lst8 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.8e-83
Identity = 163/316 (51.58%), Postives = 196/316 (62.03%), Query Frame = 0


Query: 4   PSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDI 63
           P +ILATASYDHTI+FW+  SG CYR+I   +  +NRLEIT DK Y+AAAGNP  RLF++
Sbjct: 2   PGIILATASYDHTIKFWDPPSGGCYRSIDCGEFHINRLEITHDKLYIAAAGNPQTRLFEV 61

Query: 64  NSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYRA 123
           N+N+  P MS+D H  NV  VGFQ +G WMY+GSEDGTVKIWDL              +A
Sbjct: 62  NTNNNSPAMSFDGHKGNVTGVGFQKEGKWMYTGSEDGTVKIWDL--------------KA 121

Query: 124 PGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLG-IGFRM 183
           PGCQR+YE  A VNTVVLHPNQ ELISGDQNG+IRVWDL +N+CS EL  VP G +G   
Sbjct: 122 PGCQRDYECSAPVNTVVLHPNQAELISGDQNGSIRVWDLISNTCSREL--VPDGEVGITS 181

Query: 184 EVLSVD---EEKSSSKG--------------------LDKEN-----KLMKEQCGYLATA 243
             +S D      S++KG                    ++  N      L       LAT 
Sbjct: 182 LTISSDGGLVVASNTKGKCFVWRLGEDDTSRFEPLQKIEAHNAPILKTLFSPDTKLLATC 241

Query: 244 SSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEMK 291
           S+D TVKIWN   F + +TL GHQRWVWDC FS D AYL+T SSD  +RLW +  G  +K
Sbjct: 242 SADHTVKIWNTKKFNVVQTLNGHQRWVWDCAFSNDSAYLVTGSSDHLSRLWDLHQGDAVK 301

[Close]

BLAST of HG10019934 vs. ExPASy Swiss-Prot
Match: O74184 (Target of rapamycin complex subunit wat1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pop3 PE=1 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 1.5e-81
Identity = 150/318 (47.17%), Postives = 203/318 (63.84%), Query Frame = 0


Query: 3   QPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFD 62
           Q SV+L ++ YDHTIRFWEA SG C RTIQ+ DSQVNRL I+PDK++LAAAGNPH+RL+D
Sbjct: 8   QHSVLLVSSGYDHTIRFWEALSGICSRTIQHADSQVNRLCISPDKKFLAAAGNPHVRLYD 67

Query: 63  INSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYR 122
           IN++S  P+M+++ HTNNV ++ F CDG W+ + SEDGTVK+WD+              R
Sbjct: 68  INTSSQMPLMTFEGHTNNVTAIAFHCDGKWLATSSEDGTVKVWDM--------------R 127

Query: 123 APGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV---HVPLG-- 182
           AP  QR Y+ ++ VN +++HPNQ EL+S DQ+G +R WDL  NSC+ EL+    VP+   
Sbjct: 128 APSVQRNYDHKSPVNDLLIHPNQGELLSCDQSGRVRAWDLGENSCTHELIPEEDVPMSSI 187

Query: 183 -IGFRMEVLSVDEEKS--------SSKGLDKENKLMKEQC--------------GYLATA 242
            +G    +L     K         + +G      ++K Q                +LAT 
Sbjct: 188 TVGSDGSMLIAGNNKGNCYVWRMLNHQGASLLQPVVKFQAHQRYITRCVLSPDVKHLATC 247

Query: 243 SSDSTVKIWNVD--GFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKE 291
           S+D+TV IW+ +   F LE+ L GHQRWVWDC FS D  YL+TASSD  ARLW +S+G+ 
Sbjct: 248 SADATVNIWSTEDMSFMLERRLQGHQRWVWDCAFSADSTYLVTASSDHVARLWELSSGET 307

[Close]

BLAST of HG10019934 vs. ExPASy TrEMBL
Match: A0A5D3DPG8 (Target of rapamycin complex subunit LST8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005750 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 252/330 (76.36%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. ExPASy TrEMBL
Match: A0A0A0LH11 (Target of rapamycin complex subunit LST8 OS=Cucumis sativus OX=3659 GN=Csa_3G811590 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 252/330 (76.36%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. ExPASy TrEMBL
Match: A0A1S3B820 (Target of rapamycin complex subunit LST8 OS=Cucumis melo OX=3656 GN=LOC103486798 PE=3 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 3.2e-135
Identity = 252/330 (76.36%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. ExPASy TrEMBL
Match: A0A6J1DLL8 (Target of rapamycin complex subunit LST8 OS=Momordica charantia OX=3673 GN=LOC111021575 PE=3 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 7.2e-135
Identity = 251/330 (76.06%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVN+LEITPDKRYLAAAGNPHIRL
Sbjct: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNKLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. ExPASy TrEMBL
Match: A0A6J1FAK4 (Target of rapamycin complex subunit LST8 OS=Cucurbita moschata OX=3662 GN=LOC111442300 PE=3 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 1.6e-134
Identity = 250/330 (75.76%), Postives = 257/330 (77.88%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FD+NSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL             
Sbjct: 61  FDLNSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGF 180
            RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV   +    
Sbjct: 121 -RAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV-PEVDTAV 180

Query: 181 RMEVLSVD-------------------------------EEKSSSKGLDKENKLMKEQC- 240
           R   +  D                                +  + KG   +  L  E C 
Sbjct: 181 RSLTVMWDGSLVVAANNHGTCYVWRLLRGTQTMTNFEPLHKLQAHKGYILKCLLSPEFCE 240

Query: 241 --GYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 297
              YLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS
Sbjct: 241 PHRYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWS 300

[Close]

BLAST of HG10019934 vs. TAIR 10
Match: AT3G18140.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 434.1 bits (1115), Expect = 9.0e-122
Identity = 217/319 (68.03%), Postives = 235/319 (73.67%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEA++GRCYRTIQYPDS VNRLEITPDK YLAAA NPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAETGRCYRTIQYPDSHVNRLEITPDKHYLAAACNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FD+NSNSPQP+M+YDSHTNNVM+VGFQCD  WMYSGSEDGTVKIWDL             
Sbjct: 61  FDVNSNSPQPVMTYDSHTNNVMAVGFQCDAKWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHV------ 180
            RAPGCQ+EYES AAVNTVVLHPNQTELISGDQNGNIRVWDL ANSCSCELV        
Sbjct: 121 -RAPGCQKEYESVAAVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELVPEVDTAVR 180

Query: 181 ---------------PLGIGFRMEVLSVDEEKSSSKGLDKENK--------LMKEQCGYL 240
                            G  +   +L   +  +  + L K           L+     YL
Sbjct: 181 SLTVMWDGTMVVAANNRGTCYVWRLLRGKQTMTEFEPLHKLQAHNGHILKCLLSPANKYL 240

Query: 241 ATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGK 291
           ATASSD TVKIWNVDGF LEK L GHQRWVWDCVFSVDG +L+TASSD TARLWSM  GK
Sbjct: 241 ATASSDKTVKIWNVDGFKLEKVLTGHQRWVWDCVFSVDGEFLVTASSDMTARLWSMPAGK 300

[Close]

BLAST of HG10019934 vs. TAIR 10
Match: AT2G22040.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.1e-90
Identity = 176/315 (55.87%), Postives = 208/315 (66.03%), Query Frame = 0


Query: 6   VILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRLFDINS 65
           V LATAS+D TIR W+A++GRCY + +YPD  VNRLE+TP+K  L AA NPHIRLFD+ S
Sbjct: 11  VYLATASHDQTIRLWQARTGRCYFSFRYPDLHVNRLELTPEKGKLVAACNPHIRLFDLRS 70

Query: 66  NSPQ-PIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYRAP 125
            +P  P+ ++ SHT NVM+VGFQ  G+ MYSGSEDG+VKIWDLR+               
Sbjct: 71  YNPHIPVRNFVSHTKNVMAVGFQYTGHMMYSGSEDGSVKIWDLRVR-------------- 130

Query: 126 GCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGFR--- 185
            CQRE+ S + VNTVVLHPNQTELISGDQNGNIRVWDL A+ CSCELV   +G   R   
Sbjct: 131 ECQREFRSVSPVNTVVLHPNQTELISGDQNGNIRVWDLRADLCSCELV-PEVGTPIRSLT 190

Query: 186 ------MEVLSVDE----------EKSSSKGLDKENKLMKE-----QC-------GYLAT 245
                 M V + D           E+ +    +  +KL        +C        YLAT
Sbjct: 191 VMWDGTMVVAANDRGTCYVWRSLCERQTMTEFEPLHKLQAHNSHILKCLLSPGNNRYLAT 250

Query: 246 ASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCVFSVDGAYLITASSDTTARLWSMSTGKEM 289
           ASSD TVKIWN+DGF LEK L GH+RWVWDC FS+DG YL+TASSDTTARLWSM  GKE 
Sbjct: 251 ASSDKTVKIWNLDGFKLEKVLTGHERWVWDCDFSMDGEYLVTASSDTTARLWSMRAGKEE 310

[Close]

BLAST of HG10019934 vs. TAIR 10
Match: AT3G18140.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 303.9 bits (777), Expect = 1.4e-82
Identity = 143/172 (83.14%), Postives = 151/172 (87.79%), Query Frame = 0


Query: 1   MTQPSVILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNPHIRL 60
           M+QPSVILATASYDHTIRFWEA++GRCYRTIQYPDS VNRLEITPDK YLAAA NPHIRL
Sbjct: 1   MSQPSVILATASYDHTIRFWEAETGRCYRTIQYPDSHVNRLEITPDKHYLAAACNPHIRL 60

Query: 61  FDINSNSPQPIMSYDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEK 120
           FD+NSNSPQP+M+YDSHTNNVM+VGFQCD  WMYSGSEDGTVKIWDL             
Sbjct: 61  FDVNSNSPQPVMTYDSHTNNVMAVGFQCDAKWMYSGSEDGTVKIWDL------------- 120

Query: 121 YRAPGCQREYESRAAVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELV 173
            RAPGCQ+EYES AAVNTVVLHPNQTELISGDQNGNIRVWDL ANSCSCELV
Sbjct: 121 -RAPGCQKEYESVAAVNTVVLHPNQTELISGDQNGNIRVWDLRANSCSCELV 158

[Close]

BLAST of HG10019934 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 3.4e-20
Identity = 67/262 (25.57%), Postives = 119/262 (45.42%), Query Frame = 0


Query: 29  RTIQYPDSQVNRLEITPDKRYLA-AAGNPHIRLFDINS-NSP--QPIMSYDSHTNNVMSV 88
           +T+   +  V+ ++ + D R LA A+ +  IR + IN+ N P  +P+  +  H N +  V
Sbjct: 18  QTLTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPIAEPVQEFTGHENGISDV 77

Query: 89  GFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYRAPGCQREYESRAAVNTVVLHPN 148
            F  D  ++ S S+D T+K+WD+  T  +I T         C            V  +P 
Sbjct: 78  AFSSDARFIVSASDDKTLKLWDVE-TGSLIKTLIGHTNYAFC------------VNFNPQ 137

Query: 149 QTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGFRMEVLSVDEEKSSSKGLDKENKLM 208
              ++SG  +  +R+WD+T   C              ++VL    +  ++   +++  L 
Sbjct: 138 SNMIVSGSFDETVRIWDVTTGKC--------------LKVLPAHSDPVTAVDFNRDGSL- 197

Query: 209 KEQCGYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWDCV-FSVDGAYLITASSDTTAR 268
                 + ++S D   +IW+       KTLI  +      V FS +G +++  + D T R
Sbjct: 198 ------IVSSSYDGLCRIWDSGTGHCVKTLIDDENPPVSFVRFSPNGKFILVGTLDNTLR 245

Query: 269 LWSMSTGKEMKVYQGHHKATIC 286
           LW++S+ K +K Y GH  A  C
Sbjct: 258 LWNISSAKFLKTYTGHVNAQYC 245

[Close]

BLAST of HG10019934 vs. TAIR 10
Match: AT4G02730.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 6.7e-16
Identity = 69/290 (23.79%), Postives = 127/290 (43.79%), Query Frame = 0


Query: 7   ILATASYDHTIRFWEAKSGRCYRTIQYPDSQVNRLEITPDKRYLAAAGNP-HIRLFDINS 66
           +LA+AS D T+  W A +       +   S ++ L  + D  Y  +A +   +R++D  +
Sbjct: 57  LLASASVDKTMILWSATNYSLIHRYEGHSSGISDLAWSSDSHYTCSASDDCTLRIWD--A 116

Query: 67  NSPQPIMS-YDSHTNNVMSVGFQCDGNWMYSGSEDGTVKIWDLRLTLKVIWTYFEKYRAP 126
            SP   +     HTN V  V F    N + SGS D T++IW++              +  
Sbjct: 117 RSPYECLKVLRGHTNFVFCVNFNPPSNLIVSGSFDETIRIWEV--------------KTG 176

Query: 127 GCQREYESRA-AVNTVVLHPNQTELISGDQNGNIRVWDLTANSCSCELVHVPLGIGFRME 186
            C R  ++ +  +++V  + + + ++S   +G+ ++WD    +C   L+           
Sbjct: 177 KCVRMIKAHSMPISSVHFNRDGSLIVSASHDGSCKIWDAKEGTCLKTLI----------- 236

Query: 187 VLSVDEEKSSSKGLDKENKLMKEQCGYLATASSDSTVKIWNVDGFTLEKTLIGHQRWVWD 246
                ++KS +    K +   K    ++  A+ DST+K+ N       K   GH   V+ 
Sbjct: 237 -----DDKSPAVSFAKFSPNGK----FILVATLDSTLKLSNYATGKFLKVYTGHTNKVF- 296

Query: 247 CV---FSV-DGAYLITASSDTTARLWSMSTGKEMKVYQGHHKATICCALH 290
           C+   FSV +G Y+++ S D    LW +     ++  +GH  A I  + H
Sbjct: 297 CITSAFSVTNGKYIVSGSEDNCVYLWDLQARNILQRLEGHTDAVISVSCH 309

The following BLAST results are available for this feature:

BLAST of HG10019934 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038906292.1	7.9e-136	76.44	target of rapamycin complex subunit LST8-1 [Benincasa hispida] >XP_038906293.1 t...	[more]
XP_004136587.1	6.7e-135	76.36	target of rapamycin complex subunit LST8-1 [Cucumis sativus] >XP_008443103.1 PRE...	[more]
XP_023528357.1	6.7e-135	76.36	protein LST8 homolog [Cucurbita pepo subsp. pepo] >XP_023528358.1 protein LST8 h...	[more]
XP_022154284.1	1.5e-134	76.06	protein LST8 homolog [Momordica charantia]	[more]
XP_022935415.1	3.3e-134	75.76	protein LST8 homolog isoform X1 [Cucurbita moschata] >XP_022935416.1 protein LST...	[more]

BLAST of HG10019934 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LV27	1.3e-120	68.03	Target of rapamycin complex subunit LST8-1 OS=Arabidopsis thaliana OX=3702 GN=LS...	[more]
Q9AYE4	5.0e-117	65.34	Target of rapamycin complex subunit LST8 OS=Oryza sativa subsp. japonica OX=3994...	[more]
F4IIK6	1.5e-89	55.87	Non-functional target of rapamycin complex subunit LST8-2 OS=Arabidopsis thalian...	[more]
Q54D08	2.8e-83	51.58	Protein LST8 homolog OS=Dictyostelium discoideum OX=44689 GN=lst8 PE=1 SV=1	[more]
O74184	1.5e-81	47.17	Target of rapamycin complex subunit wat1 OS=Schizosaccharomyces pombe (strain 97...	[more]

BLAST of HG10019934 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DPG8	3.2e-135	76.36	Target of rapamycin complex subunit LST8 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A0A0LH11	3.2e-135	76.36	Target of rapamycin complex subunit LST8 OS=Cucumis sativus OX=3659 GN=Csa_3G811...	[more]
A0A1S3B820	3.2e-135	76.36	Target of rapamycin complex subunit LST8 OS=Cucumis melo OX=3656 GN=LOC103486798...	[more]
A0A6J1DLL8	7.2e-135	76.06	Target of rapamycin complex subunit LST8 OS=Momordica charantia OX=3673 GN=LOC11...	[more]
A0A6J1FAK4	1.6e-134	75.76	Target of rapamycin complex subunit LST8 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of HG10019934 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G18140.1	9.0e-122	68.03	Transducin/WD40 repeat-like superfamily protein	[more]
AT2G22040.1	1.1e-90	55.87	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G18140.2	1.4e-82	83.14	Transducin/WD40 repeat-like superfamily protein	[more]
AT3G49660.1	3.4e-20	25.57	Transducin/WD40 repeat-like superfamily protein	[more]
AT4G02730.1	6.7e-16	23.79	Transducin/WD40 repeat-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020472	G-protein beta WD-40 repeat	PRINTS	PR00320	GPROTEINBRPT	coord: 93..107 score: 41.78 coord: 211..225 score: 39.97 coord: 253..267 score: 31.09
IPR001680	WD40 repeat	SMART	SM00320	WD40_4	coord: 227..266 e-value: 1.4E-7 score: 41.2 coord: 67..106 e-value: 1.1E-9 score: 48.2 coord: 168..224 e-value: 11.0 score: 10.3 coord: 123..161 e-value: 3.3E-4 score: 30.0 coord: 24..62 e-value: 0.76 score: 17.7
IPR001680	WD40 repeat	PFAM	PF00400	WD40	coord: 36..62 e-value: 0.17 score: 12.8 coord: 231..266 e-value: 7.0E-6 score: 26.7 coord: 69..106 e-value: 1.5E-8 score: 35.1 coord: 126..161 e-value: 0.0092 score: 16.8
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 210..233 score: 9.271977
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 1..30 score: 10.074014
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 234..275 score: 13.516088
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 129..170 score: 10.608706
IPR001680	WD40 repeat	PROSITE	PS50082	WD_REPEATS_2	coord: 74..108 score: 15.220417
IPR015943	WD40/YVTN repeat-like-containing domain superfamily	GENE3D	2.130.10.10		coord: 202..291 e-value: 4.0E-25 score: 90.3 coord: 5..195 e-value: 7.2E-52 score: 178.1
IPR037588	Target of rapamycin complex subunit LST8	PANTHER	PTHR19842	G BETA-LIKE PROTEIN GBL	coord: 3..189 coord: 209..291
None	No IPR available	PANTHER	PTHR19842:SF0	TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8	coord: 3..189 coord: 209..291
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 74..108 score: 12.551949
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 234..271 score: 10.179234
None	No IPR available	PROSITE	PS50294	WD_REPEATS_REGION	coord: 129..162 score: 8.834696
None	No IPR available	CDD	cd00200	WD40	coord: 7..290 e-value: 3.7288E-49 score: 163.275
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 93..107
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 8..22
IPR019775	WD40 repeat, conserved site	PROSITE	PS00678	WD_REPEATS_1	coord: 148..162
IPR036322	WD40-repeat-containing domain superfamily	SUPERFAMILY	50978	WD40 repeat-like	coord: 7..287

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10019934.1	HG10019934.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032956	regulation of actin cytoskeleton organization
biological_process	GO:0031929	TOR signaling
cellular_component	GO:0031931	TORC1 complex
cellular_component	GO:0031932	TORC2 complex
molecular_function	GO:0005515	protein binding