HG10019909 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10019909 vs. NCBI nr
Match: XP_038905747.1 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 [Benincasa hispida] >XP_038905748.1 insulin-degrading enzyme-like 1, peroxisomal isoform X1 [Benincasa hispida])

HSP 1 Score: 1733.4 bits (4488), Expect = 0.0e+00
Identity = 867/976 (88.83%), Postives = 886/976 (90.78%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTHEIVKPRNDKREYRRIVL+NSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVY+KE LDKVQILVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYSKEKLDKVQILVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPNHNCN ANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYL 
Sbjct: 241 QDIPNHNCNRANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLS 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAG+SDST+NFSFFKVVIDLTDVGQEHMQDIIGLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGDSDSTVNFSFFKVVIDLTDVGQEHMQDIIGLLF 360

Query: 361 KYISLLKQSGICQWIFDE-----------------------------LYPPEDWLVGSSL 420
           KYISLLKQSG+CQWIFDE                             LYPPEDWLVGSSL
Sbjct: 361 KYISLLKQSGVCQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI  VLDQLS+DN RIFWESKKFEGETDKVEKWYGTAYSVEKIS PL+QDWMR
Sbjct: 421 PSKFNPNLIGRVLDQLSIDNVRIFWESKKFEGETDKVEKWYGTAYSVEKISVPLIQDWMR 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPASNIFIPTDLSLKGACEKV+ PNLLR SSYSTIW+KPDTMFSTPKAYVKI
Sbjct: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVKFPNLLRMSSYSTIWYKPDTMFSTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DFKCPHADISPE EVLTVIFTRLLMDYLNEYAYNAQVAGLYY INPADSGFQVTLIGYNH
Sbjct: 541 DFKCPHADISPEAEVLTVIFTRLLMDYLNEYAYNAQVAGLYYGINPADSGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLRILLETIVEKIA FSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILED +WP
Sbjct: 601 KLRILLETIVEKIAKFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDHSWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEELEILPHL AEDLDKFVP LLSSAYLECFIAGNIERNE+ESMI+HIED+FFKGPNPI
Sbjct: 661 LMEELEILPHLRAEDLDKFVPTLLSSAYLECFIAGNIERNEAESMIEHIEDMFFKGPNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
            RPLYPSQHPANRVVKL+RGI YFYSAEGLN  DENSALVHYIQVHRDEFLQNVKLQLFA
Sbjct: 721 LRPLYPSQHPANRVVKLDRGISYFYSAEGLNPNDENSALVHYIQVHRDEFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
           HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE
Sbjct: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL+EMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL
Sbjct: 841 KKLVEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSAD NQPLQPDTVKIDD+F+FR S
Sbjct: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADLNQPLQPDTVKIDDVFTFRIS 960

BLAST of HG10019909 vs. NCBI nr
Match: KAG6580840.1 (Insulin-degrading enzyme-like 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1699.1 bits (4399), Expect = 0.0e+00
Identity = 847/976 (86.78%), Postives = 879/976 (90.06%), Query Frame = 0

Query: 1    MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
            MAVGKEQVTH+IVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 972  MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 1031

Query: 61   SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
            SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN 
Sbjct: 1032 SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNV 1091

Query: 121  DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
            DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 1092 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 1151

Query: 181  -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                 GNWDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKENLDKVQ LVENIF
Sbjct: 1152 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIF 1211

Query: 241  QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
            QDIPN+NCN ANFPGQPC SEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG
Sbjct: 1212 QDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 1271

Query: 301  HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
            HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQD++GLLF
Sbjct: 1272 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLF 1331

Query: 361  KYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVGSSL 420
            KYISLLK+SGICQWIFDEL                             YPPEDWLVGSSL
Sbjct: 1332 KYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSL 1391

Query: 421  PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
            PS FNPNLI MVLDQLSVDN RIFWESK FEG+TDKVE+WYGTAYS+EKISGPL+QDW++
Sbjct: 1392 PSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQ 1451

Query: 481  SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
            SAPDVKLHLPASN+FIPTDLSLKGACEKVE P LLRKSSYSTIW+KPDTMF TPKAYVKI
Sbjct: 1452 SAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKI 1511

Query: 541  DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
            DFKCPHADISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY I+PADSGFQVTLIGYNH
Sbjct: 1512 DFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNH 1571

Query: 601  KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
            KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQ+PYQQALYYCSLIL+D  WP
Sbjct: 1572 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWP 1631

Query: 661  LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
            LMEEL ILPHLGAEDL KF P LLSSAYLEC+IAGNIERNESE+MI+HIED+FFKG NPI
Sbjct: 1632 LMEELAILPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPI 1691

Query: 721  SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
            SRPLYPSQHP NRVVKLERGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 1692 SRPLYPSQHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFA 1751

Query: 781  HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
             VAKQAAFHQLRTVEQLGYITVL+QRNDSGIRGVQFIIQSTVKGPRNIDLRVE+FLEMFE
Sbjct: 1752 LVAKQAAFHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFE 1811

Query: 841  KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
            KKL++MTI+EFKSNVNALVD KLEKFKNLREESGFYWREIADGT+KFDRRESEVAALKTL
Sbjct: 1812 KKLVDMTIEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTL 1871

Query: 901  THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
            THQDLINFFNEHIKVGAPRK+SLSVRVYGNLHSSEYSAD +QPLQPDTVKIDDIFSFRRS
Sbjct: 1872 THQDLINFFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRS 1931

BLAST of HG10019909 vs. NCBI nr
Match: XP_022982829.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1698.7 bits (4398), Expect = 0.0e+00
Identity = 846/976 (86.68%), Postives = 879/976 (90.06%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTH+IVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN 
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNV 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPN+NCN ANFPGQPC SEHLQVLVRAIPIKQGHKLRIVWPI+PEIHHYKEGPCRYLG
Sbjct: 241 QDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPISPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQD++GLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLF 360

Query: 361 KYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVGSSL 420
           KYISLLK+SGICQWIFDEL                             YPPEDWLVGSSL
Sbjct: 361 KYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI MVLDQLSVDN RIFWESK FEG+TDKVE+WYGTAYS+EKISGPL+QDW++
Sbjct: 421 PSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPASN+FIPTDLSLKGACEKVE P LLRKSSYSTIW+KPDTMF TPKAYVKI
Sbjct: 481 SAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DFKCPHADISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY I+PADSGFQVTLIGYNH
Sbjct: 541 DFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQ+PYQQALYYCSLIL+D  WP
Sbjct: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEEL ILPHLGAEDL KF P LLSSAYLEC+IAGNIERNESE+MI+HIED+FFKG NPI
Sbjct: 661 LMEELAILPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPLYPSQHP NRVVKLERGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 721 SRPLYPSQHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
            VAKQAAFHQLRTVEQLGYITVL+QRND GIRGVQF+IQSTVKGPRNIDLRVE+FLEMFE
Sbjct: 781 LVAKQAAFHQLRTVEQLGYITVLMQRNDFGIRGVQFVIQSTVKGPRNIDLRVESFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL++MTI+EFKSNVNALVD KLEKFKNLREESGFYWREIADGT+KFDRRESEVAALKTL
Sbjct: 841 KKLVDMTIEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAPRK+SLSVRVYGNLHSSEYSAD +QPLQPDTVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. NCBI nr
Match: XP_023526970.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1696.0 bits (4391), Expect = 0.0e+00
Identity = 846/976 (86.68%), Postives = 878/976 (89.96%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTH+IVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN 
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNV 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKENLDKVQ LVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPN+NCN ANFPGQPC SEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG
Sbjct: 241 QDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQD++GLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLF 360

Query: 361 KYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVGSSL 420
           KYISLLK+SGICQWIFDEL                             YPPEDWLVGSSL
Sbjct: 361 KYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI MVLDQLSVDN RIFWESK FEG+TDKVE+WYGTAYS+EKISGPL+QDW++
Sbjct: 421 PSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPASN+FIPTDLSLKGACEKVE P LLRKSSYSTIW+KPDTMF TPKAYVKI
Sbjct: 481 SAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DFKCPHADISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY I+PADSGFQVTLIGYNH
Sbjct: 541 DFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQ+PYQQALYYCSLIL+D  WP
Sbjct: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEEL ILPHLGAEDL KF P LLSSAYLEC+IAGNIERNESE+MI+HIED+FFKG NPI
Sbjct: 661 LMEELAILPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPLYPSQHP NRVVKLERGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 721 SRPLYPSQHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
            VAKQAAFHQLRTVEQLGYITVL+QRNDSGIRGVQFIIQSTVKGPRNIDLRVE+FLEMFE
Sbjct: 781 LVAKQAAFHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL++MTI+EFKSNVNALVD KLEKFKNLREESGFYWREIADGT+KFDRRESEVAALKTL
Sbjct: 841 KKLVDMTIEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGA RK+SLSVRVYGNLHSSEYSAD +QPLQPDTVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAARKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. NCBI nr
Match: XP_022934728.1 (insulin-degrading enzyme-like 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1695.6 bits (4390), Expect = 0.0e+00
Identity = 845/976 (86.58%), Postives = 878/976 (89.96%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTH+IVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN 
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNV 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKENLDKVQ LVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPN+NCN ANFPGQPC SEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG
Sbjct: 241 QDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQD++GLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLF 360

Query: 361 KYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVGSSL 420
           KYISLLK+SGICQWIFDEL                             YPPEDWLVGSSL
Sbjct: 361 KYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI MVLDQLSVDN RIFWESK FEG+TD+VE+WYGTAYS+EKISGPL+QDW++
Sbjct: 421 PSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDRVEQWYGTAYSIEKISGPLIQDWIQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPASN+FIPTDLSLKGACEKVE P LLRKSSYSTIW+KPDTMF TPKAYVKI
Sbjct: 481 SAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DFKCPHADISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY I+PADSGFQVTLIGYNH
Sbjct: 541 DFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLRILLETIVEKIA FSVKPDRFLVIKETVLKEYQNFKFQ+PYQQALYYCSLIL+D  WP
Sbjct: 601 KLRILLETIVEKIAKFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEEL ILPHLGAEDL KF P LLSSAYLEC+IAGNIERNESE+MI+HIED+FFKG NPI
Sbjct: 661 LMEELAILPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPLYPSQHP NRVVKLERGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 721 SRPLYPSQHPTNRVVKLERGIGYFYSAEGLNRNDENSALVHYIQVHRDDFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
            VAKQAAFHQLRTVEQLGYITVL+QRNDSGIRGVQFIIQSTVKGPRNIDLRVE+FLEMFE
Sbjct: 781 LVAKQAAFHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL++MTI+EFKSNVNALVD KLEKFKNLREESGFYWREIADGT+KFDRRESEVAALKTL
Sbjct: 841 KKLVDMTIEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAPRK+SLSVRVYGNLHSSEYSAD +QPLQPDTVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. ExPASy Swiss-Prot
Match: O22941 (Insulin-degrading enzyme-like 1, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=PXM16 PE=2 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 644/979 (65.78%), Postives = 763/979 (77.94%), Query Frame = 0

Query: 1   MAVGKEQVT---HEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTV 60
           MAV K   T    EI+KPR D REYR IVL+N L+VLL+SDPDTDK        AASM+V
Sbjct: 1   MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKC-------AASMSV 60

Query: 61  NVGSFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFE 120
           +VGSFSDP+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+TASE+TNY+F+
Sbjct: 61  SVGSFSDPQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFD 120

Query: 121 VNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDS------------------------ 180
           VNADCF+EALDRFAQFFIKPLMS DATMREIKAVDS                        
Sbjct: 121 VNADCFDEALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKED 180

Query: 181 --------GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVE 240
                   GN DTL V+P+AKG+DTR EL+KFYE +YS+N+MHLVVY KE+LDK+Q LVE
Sbjct: 181 HPYHKFSTGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVE 240

Query: 241 NIFQDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCR 300
            +FQ+I N N     FPGQPCT++HLQ+LV+AIPIKQGHKL + WP+TP IHHY E P +
Sbjct: 241 RMFQEIQNTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQ 300

Query: 301 YLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIG 360
           YLGHLIGHEGEGSL++ LKTLGWATGLSAGE + T+++SFFKV IDLTD G EHMQ+I+G
Sbjct: 301 YLGHLIGHEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILG 360

Query: 361 LLFKYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVG 420
           LLF YI LL+Q+G+CQWIFDEL                             YP +DWLVG
Sbjct: 361 LLFNYIQLLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVG 420

Query: 421 SSLPSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQD 480
           SSLP+ FNP +++ V+D+LS  NFRIFWES+KFEG+TDK E WY TAYS+EKI+   +Q+
Sbjct: 421 SSLPTKFNPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQE 480

Query: 481 WMRSAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAY 540
           W++SAPDV LHLPA N+FIPTDLSLK A +K  VP LLRK+ +S +W+KPDTMFS PKAY
Sbjct: 481 WVQSAPDVHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAY 540

Query: 541 VKIDFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIG 600
           VK+DF CP A  SP+  VLT IFTRLLMDYLNEYAY AQVAGLYY ++ +D+GF++TL+G
Sbjct: 541 VKMDFNCPLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLG 600

Query: 601 YNHKLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQ 660
           YNHKLRILLET+V KIANF VKPDRF VIKETV KEYQN+KF+QPY QA+YYCSLIL+DQ
Sbjct: 601 YNHKLRILLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQ 660

Query: 661 AWPLMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGP 720
            WP  EEL++L HL AED+ KFVP LLS  ++EC+IAGN+E NE+ESM+ HIED+ F  P
Sbjct: 661 TWPWTEELDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDP 720

Query: 721 NPISRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQ 780
            PI RPL+PSQH  NRVVKL  G+ YFY  +G N  DENSALVHYIQVHRD+F  N+KLQ
Sbjct: 721 KPICRPLFPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQ 780

Query: 781 LFAHVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLE 840
           LF  VAKQA FHQLRTVEQLGYIT L QRNDSGI GVQFIIQS+VKGP +ID RVE+ L+
Sbjct: 781 LFGLVAKQATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLK 840

Query: 841 MFEKKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAAL 900
            FE KL EM+ ++FKSNV AL+D KLEK KNL+EES FYWREI  GTLKF+R+E+EV+AL
Sbjct: 841 NFESKLYEMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSAL 900

Query: 901 KTLTHQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSF 916
           K L  Q+LI+FF+E+IKVGA RKKSLS+RVYG+ H  E ++D ++   P +V+I+DI  F
Sbjct: 901 KQLQKQELIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSP-SVEIEDIVGF 960

BLAST of HG10019909 vs. ExPASy Swiss-Prot
Match: F4J3D9 (Insulin-degrading enzyme-like 2 OS=Arabidopsis thaliana OX=3702 GN=At3g57470 PE=3 SV=2)

HSP 1 Score: 1174.1 bits (3036), Expect = 0.0e+00
Identity = 596/973 (61.25%), Postives = 713/973 (73.28%), Query Frame = 0

Query: 1   MAVGKEQVT-----HEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASM 60
           MAVG E  T      EI+KPR DKREYRRIVL+NSLEVLL+SDP+TDK        AASM
Sbjct: 1   MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKC-------AASM 60

Query: 61  TVNVGSFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYY 120
            V+VGSF+DPEGLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+
Sbjct: 61  NVSVGSFTDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYH 120

Query: 121 FEVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDS---------------------- 180
           F++N D F EALDRFAQFFI+PLMS DATMREIKAVDS                      
Sbjct: 121 FDINTDSFYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSR 180

Query: 181 ----------GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQIL 240
                     GN DTL V+P+  G+DTR EL+KFY+ +YS+N+MHLVVY KENLDK Q L
Sbjct: 181 EDHPYHKFSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGL 240

Query: 241 VENIFQDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGP 300
           VE +FQ I N N     FPGQPCT +HLQVLV+A+PI QGH+L + WP+TP I HY+E P
Sbjct: 241 VEALFQGIRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAP 300

Query: 301 CRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDI 360
           CRYLG LIGHEGEGSL++ LK LGWATGL AGE+D +M +SFF V IDLTD G EHMQDI
Sbjct: 301 CRYLGDLIGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDI 360

Query: 361 IGLLFKYISLLKQSGICQWIFDEL-----------------------------YPPEDWL 420
           +GLLF+YI +L+QSG+ QWIFDEL                             YP + WL
Sbjct: 361 LGLLFEYIKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWL 420

Query: 421 VGSSLPSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLL 480
           VGSSLPS FNP +++ VLD+LS +N RIFWES KFEG+TDKVE WY TAYS+EKI+   +
Sbjct: 421 VGSSLPSKFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTI 480

Query: 481 QDWMRSAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPK 540
           Q+WM+SAPDV L LP  N+FIPTD SLK   +K   P LLRK+SYS +W+KPDT F  PK
Sbjct: 481 QEWMQSAPDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPK 540

Query: 541 AYVKIDFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTL 600
           AYVK+DF CP A  SP+  VL+ IF  LL+DYLNEYAY AQ AGL Y ++ +D+GF+++L
Sbjct: 541 AYVKMDFNCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSL 600

Query: 601 IGYNHKLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILE 660
            G+NHKLRILLE +++KIA F VKPDRF VIKETV K YQN KFQQP++QA  YCSL+L+
Sbjct: 601 AGFNHKLRILLEAVIQKIAKFEVKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQ 660

Query: 661 DQAWPLMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFK 720
           DQ WP  EEL+ L HL AEDL  FVP LLS  ++EC+IAGN+E++E+ESM+ HIED+ F 
Sbjct: 661 DQIWPWTEELDALSHLEAEDLANFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFT 720

Query: 721 GPNPISRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVK 780
              PI RPL+PSQ   NRV +L  G+ +FY  EG NS DENSALVHYIQVH+DEF  N K
Sbjct: 721 DSKPICRPLFPSQFLTNRVTELGTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSK 780

Query: 781 LQLFAHVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAF 840
           LQLF  +AKQ  FHQLRT+EQLGYIT L   NDSG+ GVQFIIQS+VKGP +ID RVE+ 
Sbjct: 781 LQLFELIAKQDTFHQLRTIEQLGYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESL 840

Query: 841 LEMFEKKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVA 900
           L+  E K   M+ +EFKSNV  L+D KLEK KNL EES FYW EI  GTLKF+R ++EVA
Sbjct: 841 LKDLESKFYNMSDEEFKSNVTNLIDMKLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVA 900

Query: 901 ALKTLTHQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIF 908
           AL+ L   + I+FF+E+IKV AP KKSLS+ VYGN H  E   D ++ +   +++I+DI 
Sbjct: 901 ALRLLKKDEWIDFFDEYIKVDAPNKKSLSICVYGNQHLKEMRNDKDK-IPSTSIEIEDIV 960

BLAST of HG10019909 vs. ExPASy Swiss-Prot
Match: Q24K02 (Insulin-degrading enzyme OS=Bos taurus OX=9913 GN=IDE PE=2 SV=1)

HSP 1 Score: 635.2 bits (1637), Expect = 1.2e-180
Identity = 368/971 (37.90%), Postives = 522/971 (53.76%), Query Frame = 0

Query: 6    EQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVGSFSDP 65
            +++ H I+K   DKREYR + L N ++VLLVSDP TDK       S+A++ V++GS SDP
Sbjct: 48   KRIGHHIIKSHEDKREYRGLELANGIKVLLVSDPTTDK-------SSAALDVHIGSLSDP 107

Query: 66   EGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEE 125
              + GL+HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E 
Sbjct: 108  PNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEG 167

Query: 126  ALDRFAQFFIKPLMSPDATMREIKAVDS-------------------------------- 185
            ALDRFAQFF+ PL       RE+ AVDS                                
Sbjct: 168  ALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGT 227

Query: 186  GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPN 245
            GN  TLE +P  +G+D R ELLKF+   YSSN+M + V  +E+LD +  LV  +F ++ N
Sbjct: 228  GNKYTLETRPNQEGIDVRQELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVEN 287

Query: 246  HNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGH 305
             N     FP  P   EHL+ L + +PIK    L + +PI     +YK  P  YLGHLIGH
Sbjct: 288  KNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGH 347

Query: 306  EGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLFKYISL 365
            EG GSL   LK+ GW   L  G+ +    F FF + +DLT+ G  H++DII  +F+YI  
Sbjct: 348  EGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQK 407

Query: 366  LKQSGICQWIFDE-----------------------------LYPPEDWLVGSSLPSNFN 425
            L+  G  +W+F E                              YP E+ L    L   F 
Sbjct: 408  LRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFR 467

Query: 426  PNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMRSAPDV 485
            P+LIEMVLD+L  +N R+   SK FEG+TD+ E+WYGT Y  E I   +++ W  +  + 
Sbjct: 468  PDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNG 527

Query: 486  KLHLPASNIFIPTDLS-LKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKIDFKC 545
            K  LP  N FIPT+   L    E    P+L++ ++ S +W K D  F  PKA +  +F  
Sbjct: 528  KFKLPMKNEFIPTNFEILSLEKEATPYPSLIKDTAMSKLWFKQDDKFFLPKACLNFEFFS 587

Query: 546  PHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNHKLRI 605
            P A + P    +  ++  LL D LNEYAY A++AGL Y +     G  +++ GYN K  I
Sbjct: 588  PFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPI 647

Query: 606  LLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWPLMEE 665
            LL+ I+EK+A F +   RF +IKE  ++   NF+ +QP+Q A+YY  L++ + AW   E 
Sbjct: 648  LLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDEL 707

Query: 666  LEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPISRPL 725
             E L  +    L  F+P LLS  ++E  + GNI +  +  ++  +ED   +  +  ++PL
Sbjct: 708  KEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAH--TKPL 767

Query: 726  YPSQHPANRVVKL-ERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFAHVA 785
             PSQ    R V+L +RG   ++  +  N    N  +  Y Q       +N+ L+LF  + 
Sbjct: 768  LPSQLVRYREVQLPDRG---WFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQII 827

Query: 786  KQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFEKKL 845
             +  F+ LRT EQLGYI     R  +GI+G++FIIQS  K P  ++ RVEAFL   EK +
Sbjct: 828  SEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSE-KPPHYLESRVEAFLITMEKSI 887

Query: 846  IEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTLTHQ 904
             +MT + F+ ++ AL   +L+K K L  E   YW EI      FDR   EVA LKTLT +
Sbjct: 888  EDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNIEVAYLKTLTKE 947

BLAST of HG10019909 vs. ExPASy Swiss-Prot
Match: P14735 (Insulin-degrading enzyme OS=Homo sapiens OX=9606 GN=IDE PE=1 SV=4)

HSP 1 Score: 629.0 bits (1621), Expect = 8.3e-179
Identity = 366/971 (37.69%), Postives = 521/971 (53.66%), Query Frame = 0

Query: 6    EQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVGSFSDP 65
            +++ + I K   DKREYR + L N ++VLL+SDP TDK       S+A++ V++GS SDP
Sbjct: 48   KRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDK-------SSAALDVHIGSLSDP 107

Query: 66   EGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEE 125
              + GL+HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E 
Sbjct: 108  PNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEG 167

Query: 126  ALDRFAQFFIKPLMSPDATMREIKAVDS-------------------------------- 185
            ALDRFAQFF+ PL       RE+ AVDS                                
Sbjct: 168  ALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGT 227

Query: 186  GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPN 245
            GN  TLE +P  +G+D R ELLKF+   YSSN+M + V  +E+LD +  LV  +F ++ N
Sbjct: 228  GNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVEN 287

Query: 246  HNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGH 305
             N     FP  P   EHL+ L + +PIK    L + +PI     +YK  P  YLGHLIGH
Sbjct: 288  KNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGH 347

Query: 306  EGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLFKYISL 365
            EG GSL   LK+ GW   L  G+ +    F FF + +DLT+ G  H++DII  +F+YI  
Sbjct: 348  EGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQK 407

Query: 366  LKQSGICQWIFDE-----------------------------LYPPEDWLVGSSLPSNFN 425
            L+  G  +W+F E                              YP E+ L    L   F 
Sbjct: 408  LRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFR 467

Query: 426  PNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMRSAPDV 485
            P+LIEMVLD+L  +N R+   SK FEG+TD+ E+WYGT Y  E I   +++ W  +  + 
Sbjct: 468  PDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNG 527

Query: 486  KLHLPASNIFIPTDLS-LKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKIDFKC 545
            K  LP  N FIPT+   L    E    P L++ ++ S +W K D  F  PKA +  +F  
Sbjct: 528  KFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFS 587

Query: 546  PHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNHKLRI 605
            P A + P    +  ++  LL D LNEYAY A++AGL Y +     G  +++ GYN K  I
Sbjct: 588  PFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPI 647

Query: 606  LLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWPLMEE 665
            LL+ I+EK+A F +   RF +IKE  ++   NF+ +QP+Q A+YY  L++ + AW   E 
Sbjct: 648  LLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDEL 707

Query: 666  LEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPISRPL 725
             E L  +    L  F+P LLS  ++E  + GNI +  +  ++  +ED   +  +  ++PL
Sbjct: 708  KEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAH--TKPL 767

Query: 726  YPSQHPANRVVKL-ERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFAHVA 785
             PSQ    R V+L +RG   ++  +  N    N  +  Y Q       +N+ L+LF  + 
Sbjct: 768  LPSQLVRYREVQLPDRG---WFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQII 827

Query: 786  KQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFEKKL 845
             +  F+ LRT EQLGYI     R  +GI+G++FIIQS  K P  ++ RVEAFL   EK +
Sbjct: 828  SEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSE-KPPHYLESRVEAFLITMEKSI 887

Query: 846  IEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTLTHQ 904
             +MT + F+ ++ AL   +L+K K L  E   YW EI      FDR  +EVA LKTLT +
Sbjct: 888  EDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKE 947

BLAST of HG10019909 vs. ExPASy Swiss-Prot
Match: P35559 (Insulin-degrading enzyme OS=Rattus norvegicus OX=10116 GN=Ide PE=1 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 7.7e-177
Identity = 365/972 (37.55%), Postives = 517/972 (53.19%), Query Frame = 0

Query: 6    EQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVGSFSDP 65
            +++   IVK   DKREYR + L N ++VLL+SDP TDK       S+A++ V++GS SDP
Sbjct: 48   QRIEDHIVKSPEDKREYRGLELANGIKVLLISDPTTDK-------SSAALDVHIGSLSDP 107

Query: 66   EGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEE 125
              + GL+HF EHMLF  ++KYP E+ YS++++EH GS NAFT+ E TNYYF+V+ +  E 
Sbjct: 108  PNIPGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEG 167

Query: 126  ALDRFAQFFIKPLMSPDATMREIKAVDS-------------------------------- 185
            ALDRFAQFF+ PL       RE+ AVDS                                
Sbjct: 168  ALDRFAQFFLCPLFDASCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGT 227

Query: 186  GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPN 245
            GN  TLE +P  +G+D R ELLKF+   YSSN+M + V  +E+LD +  LV  +F ++ N
Sbjct: 228  GNKYTLETRPNQEGIDVREELLKFHSTYYSSNLMAICVLGRESLDDLTNLVVKLFSEVEN 287

Query: 246  HNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGH 305
             N     FP  P   EHL+ L + +PIK    L + +PI     +YK  P  YLGHLIGH
Sbjct: 288  KNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQQYYKSNPGHYLGHLIGH 347

Query: 306  EGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLFKYISL 365
            EG GSL   LK+ GW   L  G+ +    F FF + +DLT+ G  H++DII  +F+YI  
Sbjct: 348  EGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQK 407

Query: 366  LKQSGICQWIFDE-----------------------------LYPPEDWLVGSSLPSNFN 425
            L+  G  +W+F E                              YP    L    L   F 
Sbjct: 408  LRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGKLHYYPLNGVLTAEYLLEEFR 467

Query: 426  PNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMRSAPDV 485
            P+LI+MVLD+L  +N R+   SK FEG+TD+ E+WYGT Y  E I   ++Q W  +  + 
Sbjct: 468  PDLIDMVLDKLRPENVRVAIVSKSFEGKTDRTEQWYGTQYKQEAIPEDVIQKWQNADLNG 527

Query: 486  KLHLPASNIFIPTDLSLKGACEK--VEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKIDFK 545
            K  LP  N FIPT+  +  A EK     P L++ ++ S +W K D  F  PKA +  +F 
Sbjct: 528  KFKLPTKNEFIPTNFEIL-ALEKDATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFF 587

Query: 546  CPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNHKLR 605
             P A + P    +  ++  LL D LNEYAY A++AGL Y +     G  +++ GYN K  
Sbjct: 588  SPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQP 647

Query: 606  ILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWPLME 665
            ILL+ I EK+A F +   RF +IKE  ++   NF+ +QP+Q A+YY  L++ + AW   E
Sbjct: 648  ILLKKITEKMATFEIDKKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDE 707

Query: 666  ELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPISRP 725
              E L  +    L  F+P LLS  ++E  + GNI +  +  ++  +ED   +  +  ++P
Sbjct: 708  LKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGVMQMVEDTLIEHAH--TKP 767

Query: 726  LYPSQHPANRVVKL-ERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFAHV 785
            L PSQ    R V+L +RG   ++  +  N    N  +  Y Q       +N+ L+LF  +
Sbjct: 768  LLPSQLVRYREVQLPDRG---WFVYQRRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQI 827

Query: 786  AKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFEKK 845
              +  F+ LRT EQLGYI     R  +GI+G++FIIQS  K P  ++ RVEAFL   EK 
Sbjct: 828  ISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSE-KPPHYLESRVEAFLITMEKA 887

Query: 846  LIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTLTH 904
            + +MT + F+ ++ AL   +L+K K L  E   YW EI      +DR   EVA LKTL+ 
Sbjct: 888  IEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNYDRDNIEVAYLKTLSK 947

BLAST of HG10019909 vs. ExPASy TrEMBL
Match: A0A6J1J5M2 (insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111481567 PE=3 SV=1)

HSP 1 Score: 1698.7 bits (4398), Expect = 0.0e+00
Identity = 846/976 (86.68%), Postives = 879/976 (90.06%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTH+IVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN 
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNV 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPN+NCN ANFPGQPC SEHLQVLVRAIPIKQGHKLRIVWPI+PEIHHYKEGPCRYLG
Sbjct: 241 QDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPISPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQD++GLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLF 360

Query: 361 KYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVGSSL 420
           KYISLLK+SGICQWIFDEL                             YPPEDWLVGSSL
Sbjct: 361 KYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI MVLDQLSVDN RIFWESK FEG+TDKVE+WYGTAYS+EKISGPL+QDW++
Sbjct: 421 PSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDKVEQWYGTAYSIEKISGPLIQDWIQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPASN+FIPTDLSLKGACEKVE P LLRKSSYSTIW+KPDTMF TPKAYVKI
Sbjct: 481 SAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DFKCPHADISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY I+PADSGFQVTLIGYNH
Sbjct: 541 DFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQ+PYQQALYYCSLIL+D  WP
Sbjct: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEEL ILPHLGAEDL KF P LLSSAYLEC+IAGNIERNESE+MI+HIED+FFKG NPI
Sbjct: 661 LMEELAILPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPLYPSQHP NRVVKLERGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 721 SRPLYPSQHPTNRVVKLERGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
            VAKQAAFHQLRTVEQLGYITVL+QRND GIRGVQF+IQSTVKGPRNIDLRVE+FLEMFE
Sbjct: 781 LVAKQAAFHQLRTVEQLGYITVLMQRNDFGIRGVQFVIQSTVKGPRNIDLRVESFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL++MTI+EFKSNVNALVD KLEKFKNLREESGFYWREIADGT+KFDRRESEVAALKTL
Sbjct: 841 KKLVDMTIEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAPRK+SLSVRVYGNLHSSEYSAD +QPLQPDTVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. ExPASy TrEMBL
Match: A0A6J1F8G9 (insulin-degrading enzyme-like 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111441824 PE=3 SV=1)

HSP 1 Score: 1695.6 bits (4390), Expect = 0.0e+00
Identity = 845/976 (86.58%), Postives = 878/976 (89.96%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTH+IVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVGKEQVTHDIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVN 
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNV 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHELLKFY+NNYSSNVMHLVVYAKENLDKVQ LVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELLKFYKNNYSSNVMHLVVYAKENLDKVQSLVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPN+NCN ANFPGQPC SEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG
Sbjct: 241 QDIPNNNCNRANFPGQPCMSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKV IDLTDVGQEHMQD++GLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVGIDLTDVGQEHMQDVVGLLF 360

Query: 361 KYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVGSSL 420
           KYISLLK+SGICQWIFDEL                             YPPEDWLVGSSL
Sbjct: 361 KYISLLKESGICQWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQFYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI MVLDQLSVDN RIFWESK FEG+TD+VE+WYGTAYS+EKISGPL+QDW++
Sbjct: 421 PSKFNPNLIGMVLDQLSVDNVRIFWESKNFEGKTDRVEQWYGTAYSIEKISGPLIQDWIQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPASN+FIPTDLSLKGACEKVE P LLRKSSYSTIW+KPDTMF TPKAYVKI
Sbjct: 481 SAPDVKLHLPASNVFIPTDLSLKGACEKVEFPILLRKSSYSTIWYKPDTMFFTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DFKCPHADISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY I+PADSGFQVTLIGYNH
Sbjct: 541 DFKCPHADISPEAEVLTEIFTRLLMDYLNEYAYSAQVAGLYYGISPADSGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLRILLETIVEKIA FSVKPDRFLVIKETVLKEYQNFKFQ+PYQQALYYCSLIL+D  WP
Sbjct: 601 KLRILLETIVEKIAKFSVKPDRFLVIKETVLKEYQNFKFQKPYQQALYYCSLILQDHTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEEL ILPHLGAEDL KF P LLSSAYLEC+IAGNIERNESE+MI+HIED+FFKG NPI
Sbjct: 661 LMEELAILPHLGAEDLVKFAPTLLSSAYLECYIAGNIERNESETMIEHIEDMFFKGTNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPLYPSQHP NRVVKLERGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 721 SRPLYPSQHPTNRVVKLERGIGYFYSAEGLNRNDENSALVHYIQVHRDDFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
            VAKQAAFHQLRTVEQLGYITVL+QRNDSGIRGVQFIIQSTVKGPRNIDLRVE+FLEMFE
Sbjct: 781 LVAKQAAFHQLRTVEQLGYITVLMQRNDSGIRGVQFIIQSTVKGPRNIDLRVESFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL++MTI+EFKSNVNALVD KLEKFKNLREESGFYWREIADGT+KFDRRESEVAALKTL
Sbjct: 841 KKLVDMTIEEFKSNVNALVDMKLEKFKNLREESGFYWREIADGTMKFDRRESEVAALKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAPRK+SLSVRVYGNLHSSEYSAD +QPLQPDTVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAPRKRSLSVRVYGNLHSSEYSADLDQPLQPDTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. ExPASy TrEMBL
Match: A0A1S3B7B5 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo OX=3656 GN=LOC103486819 PE=3 SV=1)

HSP 1 Score: 1661.0 bits (4300), Expect = 0.0e+00
Identity = 831/976 (85.14%), Postives = 865/976 (88.63%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTHEIVKPRNDKREYRRIVL+NSLEVLLVSDPDTDK       SAASMTV+VG
Sbjct: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDK-------SAASMTVDVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSMNAFTASE+TNYYF+VNA
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNA 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNW+TLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKE LDKVQILVENIF
Sbjct: 181 HKFSTGNWETLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPNHNCN ANFPGQPCTSEHLQVLVRAIPIK+GHKLRI+WPITPEIHHYKEGPCRYLG
Sbjct: 241 QDIPNHNCNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQ++IGLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLF 360

Query: 361 KYISLLKQSGICQWIFDE-----------------------------LYPPEDWLVGSSL 420
           +YISLLKQSGICQWIFDE                             LYPPEDWLVGSSL
Sbjct: 361 RYISLLKQSGICQWIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS F+P LI  VLDQLSVDN RIFWESKKFEG+TDKVEKWYGTAYS+EKISG L+QDWM+
Sbjct: 421 PSKFDPKLIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDV LHLPASNIFIPTDLSLK ACEKVE P LLRKSSYS+IW+KPDTMFSTPKAYVKI
Sbjct: 481 SAPDVNLHLPASNIFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DF CPHADISPE EVLT IFTRLLMDYLNEYAY A+VAGLYY IN  DSGFQVTL GYNH
Sbjct: 541 DFICPHADISPEAEVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQVTLNGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLR+LLETIVEKIA FSVKPDR+LVIKET+LK+YQNFKFQQPYQQALYYCSLILED+ WP
Sbjct: 601 KLRVLLETIVEKIAKFSVKPDRYLVIKETLLKDYQNFKFQQPYQQALYYCSLILEDRTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LM+EL ILPHLGA DLDKFVP LLSSAYLECFIAGNIER E+ESMIDHIED FFKGPNPI
Sbjct: 661 LMDELAILPHLGARDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDTFFKGPNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPL PSQ+PANR+VKLERGIGY YSAEGLNS DENSALVHYIQVHRDEFLQNVKLQLFA
Sbjct: 721 SRPLNPSQYPANRIVKLERGIGYLYSAEGLNSNDENSALVHYIQVHRDEFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
           HVAKQAAFHQLRTVEQLGYITVL Q N  GIRGVQFIIQSTVKGPR IDLRVEAFL+MFE
Sbjct: 781 HVAKQAAFHQLRTVEQLGYITVLAQSNHCGIRGVQFIIQSTVKGPRGIDLRVEAFLDMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL+EMTIDEFKSNVNALVDAKLEKFKNL+EES FYW EI+ GTLKFDRRESEVA LKTL
Sbjct: 841 KKLVEMTIDEFKSNVNALVDAKLEKFKNLKEESRFYWGEISGGTLKFDRRESEVALLKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAP KKSLSVRVYGNLHSSEYS D NQPLQP+TVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAPCKKSLSVRVYGNLHSSEYSGDLNQPLQPNTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. ExPASy TrEMBL
Match: A0A5A7TLF9 (Insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G005990 PE=3 SV=1)

HSP 1 Score: 1661.0 bits (4300), Expect = 0.0e+00
Identity = 831/976 (85.14%), Postives = 865/976 (88.63%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAVGKEQVTHEIVKPRNDKREYRRIVL+NSLEVLLVSDPDTDK       SAASMTV+VG
Sbjct: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLKNSLEVLLVSDPDTDK-------SAASMTVDVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDPEGLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGSMNAFTASE+TNYYF+VNA
Sbjct: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPQEDSYSKYITEHGGSMNAFTASEETNYYFDVNA 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQSHISSESHPF 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNW+TLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKE LDKVQILVENIF
Sbjct: 181 HKFSTGNWETLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKEKLDKVQILVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPNHNCN ANFPGQPCTSEHLQVLVRAIPIK+GHKLRI+WPITPEIHHYKEGPCRYLG
Sbjct: 241 QDIPNHNCNRANFPGQPCTSEHLQVLVRAIPIKEGHKLRIIWPITPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQ++IGLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQEVIGLLF 360

Query: 361 KYISLLKQSGICQWIFDE-----------------------------LYPPEDWLVGSSL 420
           +YISLLKQSGICQWIFDE                             LYPPEDWLVGSSL
Sbjct: 361 RYISLLKQSGICQWIFDELSAICETKFHYADKIRPINYVISLSLNMQLYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS F+P LI  VLDQLSVDN RIFWESKKFEG+TDKVEKWYGTAYS+EKISG L+QDWM+
Sbjct: 421 PSKFDPKLIGTVLDQLSVDNVRIFWESKKFEGKTDKVEKWYGTAYSIEKISGHLVQDWMQ 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDV LHLPASNIFIPTDLSLK ACEKVE P LLRKSSYS+IW+KPDTMFSTPKAYVKI
Sbjct: 481 SAPDVNLHLPASNIFIPTDLSLKRACEKVEFPVLLRKSSYSSIWYKPDTMFSTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DF CPHADISPE EVLT IFTRLLMDYLNEYAY A+VAGLYY IN  DSGFQVTL GYNH
Sbjct: 541 DFICPHADISPEAEVLTAIFTRLLMDYLNEYAYYAEVAGLYYGINLIDSGFQVTLNGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLR+LLETIVEKIA FSVKPDR+LVIKET+LK+YQNFKFQQPYQQALYYCSLILED+ WP
Sbjct: 601 KLRVLLETIVEKIAKFSVKPDRYLVIKETLLKDYQNFKFQQPYQQALYYCSLILEDRTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LM+EL ILPHLGA DLDKFVP LLSSAYLECFIAGNIER E+ESMIDHIED FFKGPNPI
Sbjct: 661 LMDELAILPHLGARDLDKFVPTLLSSAYLECFIAGNIERTEAESMIDHIEDTFFKGPNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPL PSQ+PANR+VKLERGIGY YSAEGLNS DENSALVHYIQVHRDEFLQNVKLQLFA
Sbjct: 721 SRPLNPSQYPANRIVKLERGIGYLYSAEGLNSNDENSALVHYIQVHRDEFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
           HVAKQAAFHQLRTVEQLGYITVL Q N  GIRGVQFIIQSTVKGPR IDLRVEAFL+MFE
Sbjct: 781 HVAKQAAFHQLRTVEQLGYITVLAQSNHCGIRGVQFIIQSTVKGPRGIDLRVEAFLDMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
           KKL+EMTIDEFKSNVNALVDAKLEKFKNL+EES FYW EI+ GTLKFDRRESEVA LKTL
Sbjct: 841 KKLVEMTIDEFKSNVNALVDAKLEKFKNLKEESRFYWGEISGGTLKFDRRESEVALLKTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
           THQDLINFFNEHIKVGAP KKSLSVRVYGNLHSSEYS D NQPLQP+TVKIDDIFSFRRS
Sbjct: 901 THQDLINFFNEHIKVGAPCKKSLSVRVYGNLHSSEYSGDLNQPLQPNTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. ExPASy TrEMBL
Match: A0A6J1DJ48 (insulin-degrading enzyme-like 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111021555 PE=3 SV=1)

HSP 1 Score: 1644.0 bits (4256), Expect = 0.0e+00
Identity = 815/976 (83.50%), Postives = 860/976 (88.11%), Query Frame = 0

Query: 1   MAVGKEQVTHEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTVNVG 60
           MAV KEQVTHEIVKPR DKREYRRIVLRNSLEVLLVSDPDTDK       SAASMTVNVG
Sbjct: 1   MAVVKEQVTHEIVKPRTDKREYRRIVLRNSLEVLLVSDPDTDK-------SAASMTVNVG 60

Query: 61  SFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120
           SFSDP+GLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA
Sbjct: 61  SFSDPKGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNA 120

Query: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS--------------------------- 180
           DCFEEALDRFAQFFIKPLMSPDATMREIKAVDS                           
Sbjct: 121 DCFEEALDRFAQFFIKPLMSPDATMREIKAVDSENQKNLLSDGWRMHQLQRHISSESHPY 180

Query: 181 -----GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIF 240
                GNWDTLEVQPKAKGLDTRHEL++F+E NYSSN+MHLVVYAKENLDKVQ LVENIF
Sbjct: 181 HKFSTGNWDTLEVQPKAKGLDTRHELIRFHEKNYSSNIMHLVVYAKENLDKVQSLVENIF 240

Query: 241 QDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300
           QDIPNHNCN A+FPGQPCTSEHL+VLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG
Sbjct: 241 QDIPNHNCNCAHFPGQPCTSEHLKVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLG 300

Query: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLF 360
           HLIGHEGEGSLYYVLKTLGWATGLSAGESDST NFSFFKV+IDLTD GQEHMQDI+GLLF
Sbjct: 301 HLIGHEGEGSLYYVLKTLGWATGLSAGESDSTTNFSFFKVIIDLTDAGQEHMQDIVGLLF 360

Query: 361 KYISLLKQSGICQWIFDE-----------------------------LYPPEDWLVGSSL 420
           KYI LLKQSGIC+WIFDE                             LYPPEDWLVGSSL
Sbjct: 361 KYIDLLKQSGICKWIFDELSAICETKFHYQDKIRPIDYVVNLSLNMQLYPPEDWLVGSSL 420

Query: 421 PSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR 480
           PS FNPNLI  VLDQLSV+NFRIFWESKKFEG+TDKVE WYGT YS+E ISG L+Q+WM 
Sbjct: 421 PSKFNPNLIGTVLDQLSVENFRIFWESKKFEGQTDKVEPWYGTTYSIENISGTLIQEWML 480

Query: 481 SAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKI 540
           SAPDVKLHLPA NIFIPTDLSLK A EKVE P LLRKSSYST+W+KPDTMF TPKAYVKI
Sbjct: 481 SAPDVKLHLPAPNIFIPTDLSLKDAHEKVEFPVLLRKSSYSTLWYKPDTMFFTPKAYVKI 540

Query: 541 DFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNH 600
           DF+CPHA ISPE EVLT IFTRLLMDYLNEYAY+AQVAGLYY INP ++GFQVTLIGYNH
Sbjct: 541 DFRCPHAGISPEAEVLTDIFTRLLMDYLNEYAYSAQVAGLYYGINPTEAGFQVTLIGYNH 600

Query: 601 KLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWP 660
           KLR+LLET+VEKIA F+VKPDRFLVIKETV+KEYQNFKFQQPYQQA+YYCSLIL+D  WP
Sbjct: 601 KLRVLLETVVEKIARFNVKPDRFLVIKETVMKEYQNFKFQQPYQQAMYYCSLILQDHTWP 660

Query: 661 LMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPI 720
           LMEEL ILPHLGAEDL KF P LLSSA LEC+IAGNIERNESESMI+HIED+FFKGPNPI
Sbjct: 661 LMEELAILPHLGAEDLVKFAPTLLSSACLECYIAGNIERNESESMIEHIEDIFFKGPNPI 720

Query: 721 SRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFA 780
           SRPLYPSQHP NRVVKL RGIGYFYSAEGLN  DENSALVHYIQVHRD+FLQNVKLQLFA
Sbjct: 721 SRPLYPSQHPTNRVVKLARGIGYFYSAEGLNPNDENSALVHYIQVHRDDFLQNVKLQLFA 780

Query: 781 HVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFE 840
            VAKQ+AFHQLRTVEQLGYIT L+QRNDSGIRG+QFIIQST KGPRNIDLRVEAFLEMFE
Sbjct: 781 LVAKQSAFHQLRTVEQLGYITFLMQRNDSGIRGLQFIIQSTAKGPRNIDLRVEAFLEMFE 840

Query: 841 KKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTL 900
            KL++MTIDEFKSNVNAL+D KLEKFKNLREESGFYWREI+DGTLKFDRRESEVA L+TL
Sbjct: 841 SKLVDMTIDEFKSNVNALIDMKLEKFKNLREESGFYWREISDGTLKFDRRESEVATLRTL 900

Query: 901 THQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSFRRS 916
            HQ+LINFFNEHIKVGAP KK+LSVRVYGNLHSSEYS+D +QPLQPDTVKIDDIFSFRRS
Sbjct: 901 AHQELINFFNEHIKVGAPHKKALSVRVYGNLHSSEYSSDLSQPLQPDTVKIDDIFSFRRS 960

BLAST of HG10019909 vs. TAIR 10
Match: AT2G41790.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 644/979 (65.78%), Postives = 763/979 (77.94%), Query Frame = 0

Query: 1   MAVGKEQVT---HEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASMTV 60
           MAV K   T    EI+KPR D REYR IVL+N L+VLL+SDPDTDK        AASM+V
Sbjct: 1   MAVEKSNTTVGGVEILKPRTDNREYRMIVLKNLLQVLLISDPDTDKC-------AASMSV 60

Query: 61  NVGSFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFE 120
           +VGSFSDP+GLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+TASE+TNY+F+
Sbjct: 61  SVGSFSDPQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFD 120

Query: 121 VNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDS------------------------ 180
           VNADCF+EALDRFAQFFIKPLMS DATMREIKAVDS                        
Sbjct: 121 VNADCFDEALDRFAQFFIKPLMSADATMREIKAVDSENQKNLLSDGWRIRQLQKHLSKED 180

Query: 181 --------GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVE 240
                   GN DTL V+P+AKG+DTR EL+KFYE +YS+N+MHLVVY KE+LDK+Q LVE
Sbjct: 181 HPYHKFSTGNMDTLHVRPQAKGVDTRSELIKFYEEHYSANIMHLVVYGKESLDKIQDLVE 240

Query: 241 NIFQDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCR 300
            +FQ+I N N     FPGQPCT++HLQ+LV+AIPIKQGHKL + WP+TP IHHY E P +
Sbjct: 241 RMFQEIQNTNKVVPRFPGQPCTADHLQILVKAIPIKQGHKLGVSWPVTPSIHHYDEAPSQ 300

Query: 301 YLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIG 360
           YLGHLIGHEGEGSL++ LKTLGWATGLSAGE + T+++SFFKV IDLTD G EHMQ+I+G
Sbjct: 301 YLGHLIGHEGEGSLFHALKTLGWATGLSAGEGEWTLDYSFFKVSIDLTDAGHEHMQEILG 360

Query: 361 LLFKYISLLKQSGICQWIFDEL-----------------------------YPPEDWLVG 420
           LLF YI LL+Q+G+CQWIFDEL                             YP +DWLVG
Sbjct: 361 LLFNYIQLLQQTGVCQWIFDELSAICETKFHYQDKIPPMSYIVDIASNMQIYPTKDWLVG 420

Query: 421 SSLPSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQD 480
           SSLP+ FNP +++ V+D+LS  NFRIFWES+KFEG+TDK E WY TAYS+EKI+   +Q+
Sbjct: 421 SSLPTKFNPAIVQKVVDELSPSNFRIFWESQKFEGQTDKAEPWYNTAYSLEKITSSTIQE 480

Query: 481 WMRSAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAY 540
           W++SAPDV LHLPA N+FIPTDLSLK A +K  VP LLRK+ +S +W+KPDTMFS PKAY
Sbjct: 481 WVQSAPDVHLHLPAPNVFIPTDLSLKDADDKETVPVLLRKTPFSRLWYKPDTMFSKPKAY 540

Query: 541 VKIDFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIG 600
           VK+DF CP A  SP+  VLT IFTRLLMDYLNEYAY AQVAGLYY ++ +D+GF++TL+G
Sbjct: 541 VKMDFNCPLAVSSPDAAVLTDIFTRLLMDYLNEYAYYAQVAGLYYGVSLSDNGFELTLLG 600

Query: 601 YNHKLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQ 660
           YNHKLRILLET+V KIANF VKPDRF VIKETV KEYQN+KF+QPY QA+YYCSLIL+DQ
Sbjct: 601 YNHKLRILLETVVGKIANFEVKPDRFAVIKETVTKEYQNYKFRQPYHQAMYYCSLILQDQ 660

Query: 661 AWPLMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGP 720
            WP  EEL++L HL AED+ KFVP LLS  ++EC+IAGN+E NE+ESM+ HIED+ F  P
Sbjct: 661 TWPWTEELDVLSHLEAEDVAKFVPMLLSRTFIECYIAGNVENNEAESMVKHIEDVLFNDP 720

Query: 721 NPISRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQ 780
            PI RPL+PSQH  NRVVKL  G+ YFY  +G N  DENSALVHYIQVHRD+F  N+KLQ
Sbjct: 721 KPICRPLFPSQHLTNRVVKLGEGMKYFYHQDGSNPSDENSALVHYIQVHRDDFSMNIKLQ 780

Query: 781 LFAHVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLE 840
           LF  VAKQA FHQLRTVEQLGYIT L QRNDSGI GVQFIIQS+VKGP +ID RVE+ L+
Sbjct: 781 LFGLVAKQATFHQLRTVEQLGYITALAQRNDSGIYGVQFIIQSSVKGPGHIDSRVESLLK 840

Query: 841 MFEKKLIEMTIDEFKSNVNALVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAAL 900
            FE KL EM+ ++FKSNV AL+D KLEK KNL+EES FYWREI  GTLKF+R+E+EV+AL
Sbjct: 841 NFESKLYEMSNEDFKSNVTALIDMKLEKHKNLKEESRFYWREIQSGTLKFNRKEAEVSAL 900

Query: 901 KTLTHQDLINFFNEHIKVGAPRKKSLSVRVYGNLHSSEYSADSNQPLQPDTVKIDDIFSF 916
           K L  Q+LI+FF+E+IKVGA RKKSLS+RVYG+ H  E ++D ++   P +V+I+DI  F
Sbjct: 901 KQLQKQELIDFFDEYIKVGAARKKSLSIRVYGSQHLKEMASDKDEVPSP-SVEIEDIVGF 960

BLAST of HG10019909 vs. TAIR 10
Match: AT3G57470.2 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1073.5 bits (2775), Expect = 6.5e-314
Identity = 539/891 (60.49%), Postives = 650/891 (72.95%), Query Frame = 0

Query: 78  MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKP 137
           MLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D F EALDRFAQFFI+P
Sbjct: 1   MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60

Query: 138 LMSPDATMREIKAVDS--------------------------------GNWDTLEVQPKA 197
           LMS DATMREIKAVDS                                GN DTL V+P+ 
Sbjct: 61  LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120

Query: 198 KGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNSANFPGQP 257
            G+DTR EL+KFY+ +YS+N+MHLVVY KENLDK Q LVE +FQ I N N     FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180

Query: 258 CTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKT 317
           CT +HLQVLV+A+PI QGH+L + WP+TP I HY+E PCRYLG LIGHEGEGSL++ LK 
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240

Query: 318 LGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLFKYISLLKQSGICQWIFD 377
           LGWATGL AGE+D +M +SFF V IDLTD G EHMQDI+GLLF+YI +L+QSG+ QWIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300

Query: 378 EL-----------------------------YPPEDWLVGSSLPSNFNPNLIEMVLDQLS 437
           EL                             YP + WLVGSSLPS FNP +++ VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360

Query: 438 VDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMRSAPDVKLHLPASNIFIP 497
            +N RIFWES KFEG+TDKVE WY TAYS+EKI+   +Q+WM+SAPDV L LP  N+FIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420

Query: 498 TDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKIDFKCPHADISPETEVLT 557
           TD SLK   +K   P LLRK+SYS +W+KPDT F  PKAYVK+DF CP A  SP+  VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480

Query: 558 VIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNHKLRILLETIVEKIANFS 617
            IF  LL+DYLNEYAY AQ AGL Y ++ +D+GF+++L G+NHKLRILLE +++KIA F 
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKIAKFE 540

Query: 618 VKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWPLMEELEILPHLGAEDLD 677
           VKPDRF VIKETV K YQN KFQQP++QA  YCSL+L+DQ WP  EEL+ L HL AEDL 
Sbjct: 541 VKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDALSHLEAEDLA 600

Query: 678 KFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPISRPLYPSQHPANRVVKL 737
            FVP LLS  ++EC+IAGN+E++E+ESM+ HIED+ F    PI RPL+PSQ   NRV +L
Sbjct: 601 NFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICRPLFPSQFLTNRVTEL 660

Query: 738 ERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFAHVAKQAAFHQLRTVEQL 797
             G+ +FY  EG NS DENSALVHYIQVH+DEF  N KLQLF  +AKQ  FHQLRT+EQL
Sbjct: 661 GTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELIAKQDTFHQLRTIEQL 720

Query: 798 GYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFEKKLIEMTIDEFKSNVNA 857
           GYIT L   NDSG+ GVQFIIQS+VKGP +ID RVE+ L+  E K   M+ +EFKSNV  
Sbjct: 721 GYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESKFYNMSDEEFKSNVTN 780

Query: 858 LVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTLTHQDLINFFNEHIKVGA 908
           L+D KLEK KNL EES FYW EI  GTLKF+R ++EVAAL+ L   + I+FF+E+IKV A
Sbjct: 781 LIDMKLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKKDEWIDFFDEYIKVDA 840

BLAST of HG10019909 vs. TAIR 10
Match: AT3G57470.3 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1053.9 bits (2724), Expect = 7.4e-308
Identity = 533/891 (59.82%), Postives = 643/891 (72.17%), Query Frame = 0

Query: 78  MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKP 137
           MLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+F++N D F EALDRFAQFFI+P
Sbjct: 1   MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60

Query: 138 LMSPDATMREIKAVDS--------------------------------GNWDTLEVQPKA 197
           LMS DATMREIKAVDS                                GN DTL V+P+ 
Sbjct: 61  LMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSREDHPYHKFSTGNMDTLHVRPEE 120

Query: 198 KGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNSANFPGQP 257
            G+DTR EL+KFY+ +YS+N+MHLVVY KENLDK Q LVE +FQ I N N     FPGQP
Sbjct: 121 NGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGLVEALFQGIRNTNQGIPRFPGQP 180

Query: 258 CTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKT 317
           CT +HLQVLV+A+PI QGH+L + WP+TP I HY+E PCRYLG LIGHEGEGSL++ LK 
Sbjct: 181 CTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAPCRYLGDLIGHEGEGSLFHALKI 240

Query: 318 LGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDIIGLLFKYISLLKQSGICQWIFD 377
           LGWATGL AGE+D +M +SFF V IDLTD G EHMQDI+GLLF+YI +L+QSG+ QWIFD
Sbjct: 241 LGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDILGLLFEYIKVLQQSGVSQWIFD 300

Query: 378 EL-----------------------------YPPEDWLVGSSLPSNFNPNLIEMVLDQLS 437
           EL                             YP + WLVGSSLPS FNP +++ VLD+LS
Sbjct: 301 ELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWLVGSSLPSKFNPAIVQKVLDELS 360

Query: 438 VDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMRSAPDVKLHLPASNIFIP 497
            +N RIFWES KFEG+TDKVE WY TAYS+EKI+   +Q+WM+SAPDV L LP  N+FIP
Sbjct: 361 PNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTIQEWMQSAPDVNLLLPTPNVFIP 420

Query: 498 TDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPKAYVKIDFKCPHADISPETEVLT 557
           TD SLK   +K   P LLRK+SYS +W+KPDT F  PKAYVK+DF CP A  SP+  VL+
Sbjct: 421 TDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPKAYVKMDFNCPLAVSSPDAAVLS 480

Query: 558 VIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNHKLRILLETIVEKIANFS 617
            IF  LL+DYLNEYAY AQ AGL Y ++ +D+GF+++L G+NHKLRILLE +++KIA F 
Sbjct: 481 DIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSLAGFNHKLRILLEAVIQKIAKFE 540

Query: 618 VKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWPLMEELEILPHLGAEDLD 677
           VKPDRF VIKETV K YQN KFQQP++QA  YCSL+L+DQ WP  EEL+ L HL AEDL 
Sbjct: 541 VKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQDQIWPWTEELDALSHLEAEDLA 600

Query: 678 KFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPISRPLYPSQHPANRVVKL 737
            FVP LLS  ++EC+IAGN+E++E+ESM+ HIED+ F    PI RPL+PSQ   NRV +L
Sbjct: 601 NFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFTDSKPICRPLFPSQFLTNRVTEL 660

Query: 738 ERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVKLQLFAHVAKQAAFHQLRTVEQL 797
             G+ +FY  EG NS DENSALVHYIQVH+DEF  N KLQLF  +AKQ  FHQLRT+EQL
Sbjct: 661 GTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSKLQLFELIAKQDTFHQLRTIEQL 720

Query: 798 GYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFEKKLIEMTIDEFKSNVNA 857
           GYIT L   NDSG+ GVQFIIQS+VKGP +ID RVE+ L+  E K   M+ +EF      
Sbjct: 721 GYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESLLKDLESKFYNMSDEEF------ 780

Query: 858 LVDAKLEKFKNLREESGFYWREIADGTLKFDRRESEVAALKTLTHQDLINFFNEHIKVGA 908
               KLEK KNL EES FYW EI  GTLKF+R ++EVAAL+ L   + I+FF+E+IKV A
Sbjct: 781 ----KLEKDKNLDEESWFYWAEIQTGTLKFNRIDAEVAALRLLKKDEWIDFFDEYIKVDA 840

BLAST of HG10019909 vs. TAIR 10
Match: AT3G57470.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 1051.2 bits (2717), Expect = 4.8e-307
Identity = 531/857 (61.96%), Postives = 629/857 (73.40%), Query Frame = 0

Query: 1   MAVGKEQVT-----HEIVKPRNDKREYRRIVLRNSLEVLLVSDPDTDKAKPTFAFSAASM 60
           MAVG E  T      EI+KPR DKREYRRIVL+NSLEVLL+SDP+TDK        AASM
Sbjct: 1   MAVGMENATASGECGEILKPRTDKREYRRIVLKNSLEVLLISDPETDKC-------AASM 60

Query: 61  TVNVGSFSDPEGLEGLAHFLEHMLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYY 120
            V+VGSF+DPEGLEGLAHFLEHMLFYASEKYP EDSYSKYITEHGGS NA+T+SE TNY+
Sbjct: 61  NVSVGSFTDPEGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYH 120

Query: 121 FEVNADCFEEALDRFAQFFIKPLMSPDATMREIKAVDS---------------------- 180
           F++N D F EALDRFAQFFI+PLMS DATMREIKAVDS                      
Sbjct: 121 FDINTDSFYEALDRFAQFFIQPLMSTDATMREIKAVDSEHQNNLLSDSWRMAQLQKHLSR 180

Query: 181 ----------GNWDTLEVQPKAKGLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQIL 240
                     GN DTL V+P+  G+DTR EL+KFY+ +YS+N+MHLVVY KENLDK Q L
Sbjct: 181 EDHPYHKFSTGNMDTLHVRPEENGVDTRSELIKFYDEHYSANIMHLVVYGKENLDKTQGL 240

Query: 241 VENIFQDIPNHNCNSANFPGQPCTSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGP 300
           VE +FQ I N N     FPGQPCT +HLQVLV+A+PI QGH+L + WP+TP I HY+E P
Sbjct: 241 VEALFQGIRNTNQGIPRFPGQPCTLDHLQVLVKAVPIMQGHELSVSWPVTPSISHYEEAP 300

Query: 301 CRYLGHLIGHEGEGSLYYVLKTLGWATGLSAGESDSTMNFSFFKVVIDLTDVGQEHMQDI 360
           CRYLG LIGHEGEGSL++ LK LGWATGL AGE+D +M +SFF V IDLTD G EHMQDI
Sbjct: 301 CRYLGDLIGHEGEGSLFHALKILGWATGLYAGEADWSMEYSFFNVSIDLTDAGHEHMQDI 360

Query: 361 IGLLFKYISLLKQSGICQWIFDEL-----------------------------YPPEDWL 420
           +GLLF+YI +L+QSG+ QWIFDEL                             YP + WL
Sbjct: 361 LGLLFEYIKVLQQSGVSQWIFDELSAICEAEFHYQAKIDPISYAVDISSNMKIYPTKHWL 420

Query: 421 VGSSLPSNFNPNLIEMVLDQLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLL 480
           VGSSLPS FNP +++ VLD+LS +N RIFWES KFEG+TDKVE WY TAYS+EKI+   +
Sbjct: 421 VGSSLPSKFNPAIVQKVLDELSPNNVRIFWESNKFEGQTDKVEPWYNTAYSLEKITKFTI 480

Query: 481 QDWMRSAPDVKLHLPASNIFIPTDLSLKGACEKVEVPNLLRKSSYSTIWHKPDTMFSTPK 540
           Q+WM+SAPDV L LP  N+FIPTD SLK   +K   P LLRK+SYS +W+KPDT F  PK
Sbjct: 481 QEWMQSAPDVNLLLPTPNVFIPTDFSLKDLKDKDIFPVLLRKTSYSRLWYKPDTKFFKPK 540

Query: 541 AYVKIDFKCPHADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTL 600
           AYVK+DF CP A  SP+  VL+ IF  LL+DYLNEYAY AQ AGL Y ++ +D+GF+++L
Sbjct: 541 AYVKMDFNCPLAVSSPDAAVLSDIFVWLLVDYLNEYAYYAQAAGLDYGLSLSDNGFELSL 600

Query: 601 IGYNHKLRILLETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILE 660
            G+NHKLRILLE +++KIA F VKPDRF VIKETV K YQN KFQQP++QA  YCSL+L+
Sbjct: 601 AGFNHKLRILLEAVIQKIAKFEVKPDRFSVIKETVTKAYQNNKFQQPHEQATNYCSLVLQ 660

Query: 661 DQAWPLMEELEILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFK 720
           DQ WP  EEL+ L HL AEDL  FVP LLS  ++EC+IAGN+E++E+ESM+ HIED+ F 
Sbjct: 661 DQIWPWTEELDALSHLEAEDLANFVPMLLSRTFVECYIAGNVEKDEAESMVKHIEDVLFT 720

Query: 721 GPNPISRPLYPSQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQNVK 780
              PI RPL+PSQ   NRV +L  G+ +FY  EG NS DENSALVHYIQVH+DEF  N K
Sbjct: 721 DSKPICRPLFPSQFLTNRVTELGTGMKHFYYQEGSNSSDENSALVHYIQVHKDEFSMNSK 780

Query: 781 LQLFAHVAKQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAF 792
           LQLF  +AKQ  FHQLRT+EQLGYIT L   NDSG+ GVQFIIQS+VKGP +ID RVE+ 
Sbjct: 781 LQLFELIAKQDTFHQLRTIEQLGYITSLSLSNDSGVYGVQFIIQSSVKGPGHIDSRVESL 840

BLAST of HG10019909 vs. TAIR 10
Match: AT1G06900.1 (Insulinase (Peptidase family M16) family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 5.6e-114
Identity = 289/986 (29.31%), Postives = 452/986 (45.84%), Query Frame = 0

Query: 12  IVKPRNDKREYRRIVLRNSLEVLLVSDPD------------------------------- 71
           +VK  ND+R YR I L N L  LL+ DPD                               
Sbjct: 14  VVKSPNDRRLYRVIELENGLCALLIHDPDIYPEGSVPDQIDEDDEDGEEEDSDGSSEDDD 73

Query: 72  -----------------------TDKAKPTFAFSAASMTVNVGSFSDPEGLEGLAHFLEH 131
                                    K       +AA+M V++GSF DP   +GLAHFLEH
Sbjct: 74  DDEDDEEDGEGDEEDEDEDEDEVKGKGDHQTKKAAAAMCVSMGSFLDPPEAQGLAHFLEH 133

Query: 132 MLFYASEKYPLEDSYSKYITEHGGSMNAFTASEQTNYYFEVNADCFEEALDRFAQFFIKP 191
           MLF  S ++P E+ Y  Y+++HGGS NA+T  E T Y+FEV  +  + AL RF+QFF+ P
Sbjct: 134 MLFMGSTEFPDENEYDSYLSKHGGSSNAYTEMEHTCYHFEVKREFLQGALKRFSQFFVAP 193

Query: 192 LMSPDATMREIKAVDSG----------NWDTLEVQPKAK--------------------- 251
           LM  +A  RE+ AVDS               L+    AK                     
Sbjct: 194 LMKTEAMEREVLAVDSEFNQALQNDACRLQQLQCYTSAKGHPFNRFAWGNKKSLSGAMEN 253

Query: 252 GLDTRHELLKFYENNYSSNVMHLVVYAKENLDKVQILVENIFQDIPNHNCNSANFPGQPC 311
           G+D R  ++K Y+  Y   +M LVV   E+LD ++  V  +F D+ N +        +  
Sbjct: 254 GVDLRECIVKLYKEYYHGGLMKLVVIGGESLDMLESWVVELFGDVKNGSKIRPTLEAEGP 313

Query: 312 TSEHLQVLVRAIPIKQGHKLRIVWPITPEIHHYKEGPCRYLGHLIGHEGEGSLYYVLKTL 371
             +  + L R   +K  H L + W + P    Y + P  YL HL+GHEG GSL+  LK  
Sbjct: 314 IWKGGK-LYRLEAVKDVHILDLTWTLPPLRSAYVKKPEDYLAHLLGHEGRGSLHSFLKAK 373

Query: 372 GWATGLSAGESDSTMNFS----FFKVVIDLTDVGQEHMQDIIGLLFKYISLLKQSGICQW 431
           GWAT LSAG  D  +N S     F + I LTD G E + DIIG +++Y+ LL+     +W
Sbjct: 374 GWATSLSAGVGDDGINRSSLAYVFGMSIHLTDSGLEKIYDIIGYIYQYLKLLRDVSPQEW 433

Query: 432 IFDEL-----------------------------YPPEDWLVGSSLPSNFNPNLIEMVLD 491
           IF EL                             YP E  + G  +   ++P LIE ++ 
Sbjct: 434 IFKELQDIGNMDFRFAEEQPADDYAAELSENMLAYPVEHVIYGDYVYQTWDPKLIEDLMG 493

Query: 492 QLSVDNFRIFWESKKFEGETDKVEKWYGTAYSVEKISGPLLQDWMR-SAPDVKLHLPASN 551
             +  N RI   SK  + E  + E W+G++Y  E +   L++ W   S  D  LHLP+ N
Sbjct: 494 FFTPQNMRIDVVSKSIKSEEFQQEPWFGSSYIEEDVPLSLMESWSNPSEVDNSLHLPSKN 553

Query: 552 IFIPTDLSLKGACEKVE-----VPNLLRKSSYSTIWHKPDTMFSTPKA--YVKIDFKCPH 611
            FIP D S++     V+      P  +    +   W+K D  F  P+A  Y +I+ K  +
Sbjct: 554 QFIPCDFSIRAINSDVDPKSQSPPRCIIDEPFMKFWYKLDETFKVPRANTYFRINLKGAY 613

Query: 612 ADISPETEVLTVIFTRLLMDYLNEYAYNAQVAGLYYSINPADSGFQVTLIGYNHKLRILL 671
           A +  +  +LT ++  LL D LNE  Y A +A L  S++      ++ + G+N K+  LL
Sbjct: 614 ASV--KNCLLTELYINLLKDELNEIIYQASIAKLETSLSMYGDKLELKVYGFNEKIPALL 673

Query: 672 ETIVEKIANFSVKPDRFLVIKETVLKEYQNFKFQQPYQQALYYCSLILEDQAWPLMEELE 731
             I+    +F    +RF VIKE + + ++N    +P   + Y    +L  + +   E+L 
Sbjct: 674 SKILAIAKSFMPNLERFKVIKENMERGFRNTN-MKPLNHSTYLRLQLLCKRIYDSDEKLS 733

Query: 732 ILPHLGAEDLDKFVPALLSSAYLECFIAGNIERNESESMIDHIEDLFFKGPNPISRPLYP 791
           +L  L  +DL+ F+P L S  ++E    GN+  +E+ ++ +  +D     P P       
Sbjct: 734 VLNDLSLDDLNSFIPELRSQIFIEALCHGNLSEDEAVNISNIFKDSLTVEPLPSK----- 793

Query: 792 SQHPANRVVKLERGIGYFYSAEGLNSKDENSALVHYIQVHRDEFLQ---NVKLQLFAHVA 851
            +H   ++     G          N  + NS +  Y Q+  +E         L LF  + 
Sbjct: 794 CRH-GEQITCFPMGAKLVRDVNVKNKSETNSVVELYYQIEPEEAQSTRTKAVLDLFHEII 853

Query: 852 KQAAFHQLRTVEQLGYITVLVQRNDSGIRGVQFIIQSTVKGPRNIDLRVEAFLEMFEKKL 869
           ++  F+QLRT EQLGY+     R    + G  F +QS+  GP ++  RV+ F++  E  L
Sbjct: 854 EEPLFNQLRTKEQLGYVVECGPRLTYRVHGFCFCVQSSKYGPVHLLGRVDNFIKDIEGLL 913