HG10019892 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10019892
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionmembrane steroid-binding protein 2-like
LocationChr04: 26572254 .. 26574996 (+)
RNA-Seq ExpressionHG10019892
SyntenyHG10019892
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTTCTTCTGGAAAGCAATGGAACAGATCACTTGGTACAGCGGACTATCCCCGACGGCTTTCTTCACCATACTTGCTGCGATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAGCCTCCAACTGTTCCGATTTCTTCGTCTAACCCATCCAATTCCAATTTATTTGTTAATGATTCGGTTGCAGACGCCTCGCAAGCAGTCCAAGTAGGGCGATTAACGGAGCAGCAGTTGAGGGCTTATAATGGCTCTGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGCCAAATCTACGATGTATCTTCTGGCAGGTACTCTTTCTTTTCCTCCATCGTTCGTTGGAGTGTACTTTTGTTTGGTTTCTAGAGCTTATTTACAAACTTGCTTATATTGAATCGGAGAAGTTGCTTAGTTTCATAATTGCAGCTTGGAATCAATGATCTAGATAGATAATTGCTTCTGGTAGTCACATGAACGGAGTTTAAGAAATGCTGAATAATAAAGAAAACTGACCGACCAGTGCTATTTGTTGAGGAGATACATTAGAATTGCTCGAGCATGCTTCCTGTTGTAATCCCGTTTACAGTGCTGGTCAACTTTCATATTGACTTATTGTTGAGGATCCACATTGAAAAAAATTAAGGGAACTCAAAATATTCGTGTGGGATACTCTTTCATTGGCAATTGGTATTGAGATTGAGAAGAAATCTCATGTTATCTAATTTGGTATCTAAGCTCATCAAACCCATACGGGCATTCGGTCTAAAAAAAGAATCGGCATACGACTCAAGAATGATGAACTTAAAGAGCCACCATTTTGAGGAGCATGTTGAGGCACCATCTCATATCTGTTTGACGACAAAGTTTCACTGATAATGTCTCATGACTAAGATTTTAAGGAGGCGAATGTTTTTGCACGAACTTTGTAGGTGATAAGAGTGCTAGGCCTTCCATTCGGTTTATTTATGTGAGAAGAAGGGCGGTAGGGACTAAAGAATGAAAGAGAATGAAAGTTGGGCTGTATGGGACTGGGGACTGAAATTGTTACAGAAGGCCAGGGGACTGTGTGTTTGCGTGTCAGCAGTAATAGTAGACAAATTTTAAGTCAGTCAATCGGTCTGTCAGGAAATATTTAGCTCAAGCTCGAGTTTAGGAAGTTTTGGTGTTCGAAAGCCTCCCTGTATTTATGTAATATATGCCTGGACTTTTCAAGTGAATGAGTTATAAAGGATAGCATATAGCACAAAAAATGAGTTTCATAAGCTGAAGTTCATGTAAATTAAGTCTAATATAACATATGAAATTAGTGAATTCGTTGGTTGAAATAAGCGAATACAGTCACAGATATCAAGTATAGACTTACTTCTTAACCTTGTAGAAGTTATTTTGGGGTGGGGTAAATGCAGTGATGGAAGGGGAGAAAGGTTGTTGCTAGGAGAGATCCACGCCTGTCAGCCGTTTGATGGTCTTGTTTTTCCCCATCGAAATACAAATTTTACCACTTCACAAGCGGCATTAATGGTATTAACTATTAAGAGATTTGTAGTATAAGAACTACAAGCTTGAATTTCTCTTCCAACATACTTCTGGTATGGTGGTACGAGCACGGAACTTTTGGATTTCTCAAAATTCTTAACTTCCTTAATTTAGAACTTTAGGCTTAGGTCAATTTTGTAAATTTATAGTTCCCATGTGGATTCATTACTTGGGTCAATCCATTTGCATTTAACTTTTTATCGAACTTTTCATCAATAAGTTGATAACCTCTACACGAGCACACTGGGTGTATGGTTGTGTGTTTCTTTTTCCCTCTTTTTCTTTCTTTCTTGGCCAAGAGGTACAATGTTACATTGTACGCTGGTAGCTGCCAATGACCTCTAGTTGTTGAACTTTGGTATTAACTTTTATAGCGTTCAGGTTATTATTTGATTAACCTACACACTTTGTTGTTCTTTTGTTCCTATGCTTTTACTAACCTCTAAATAGTACTTATTTTTAGACCCATGTCATTAGGTGTACAAACCAGTTTCTCTGAGCCCTAACTTTAAACCGCTTAAGTTAAATGATCCCCTTCAATCTAATTTTACCAAGCAACAATTACATTTGAGCCATAATTTTAACAAGACCGCCTGACCCATCAACACCCCAGCCCAATCAAACCTCTCAAAGCTAATGCTTATTCATTATTACCTGTTCATTTCTGATCCATCCGATTGATTTACCGTTTTACACCACCTCACCCAGAATTTAATAATTGAGTATTATGTTTGCACTTATCAACTGTTTTCAAGAGTGGGGACTTGACAAACCTAATTCTGTACGTAATTGGAGCTTCTTAGATTTCAAAGACTAAATAGAAAGTTCAATTTCCATAGATGAAATAGGTAAGTATTATTAAAGATGTTGCTGATTTTTGGTTGGCTTGATGGTCAGGATGTTTTATGGTCCTGGTTCACCGTACTCTATGTTCGTAGGAAAGGATGCCAGCAGGGCCTTGGCTCTCTTGTCTTTCAAACCTGAAGACATCAATGGTAATATCAAAGGTCTGAGTGAAGAAGAGCTTGTAATCTTGCAGGACTGGGAATACAAGTTCATGGAAAAGTATGTAAAAGTTGGGGAGCTTGTTCCAGAGGGGGGATTGAATGAGCATTCTGAAAATGGACACCAAAATTCCGAAACCGTGCGAGAAGAAGAACACGACAAAGATCAGTAA

mRNA sequence

ATGGAGTTCTTCTGGAAAGCAATGGAACAGATCACTTGGTACAGCGGACTATCCCCGACGGCTTTCTTCACCATACTTGCTGCGATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAGCCTCCAACTGTTCCGATTTCTTCGTCTAACCCATCCAATTCCAATTTATTTGTTAATGATTCGGTTGCAGACGCCTCGCAAGCAGTCCAAGTAGGGCGATTAACGGAGCAGCAGTTGAGGGCTTATAATGGCTCTGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGCCAAATCTACGATGTATCTTCTGGCAGGATGTTTTATGGTCCTGGTTCACCGTACTCTATGTTCGTAGGAAAGGATGCCAGCAGGGCCTTGGCTCTCTTGTCTTTCAAACCTGAAGACATCAATGGTAATATCAAAGGTCTGAGTGAAGAAGAGCTTGTAATCTTGCAGGACTGGGAATACAAGTTCATGGAAAAGTATGTAAAAGTTGGGGAGCTTGTTCCAGAGGGGGGATTGAATGAGCATTCTGAAAATGGACACCAAAATTCCGAAACCGTGCGAGAAGAAGAACACGACAAAGATCAGTAA

Coding sequence (CDS)

ATGGAGTTCTTCTGGAAAGCAATGGAACAGATCACTTGGTACAGCGGACTATCCCCGACGGCTTTCTTCACCATACTTGCTGCGATGATCTTCGTGTTTCAAATGGTTTCCTCCATGTTCGTTTCTCCAGAAGAGTTCAATAAGCCTCCAACTGTTCCGATTTCTTCGTCTAACCCATCCAATTCCAATTTATTTGTTAATGATTCGGTTGCAGACGCCTCGCAAGCAGTCCAAGTAGGGCGATTAACGGAGCAGCAGTTGAGGGCTTATAATGGCTCTGATCCGAATAAGCCACTTCTTATGGCTATAAAGGGCCAAATCTACGATGTATCTTCTGGCAGGATGTTTTATGGTCCTGGTTCACCGTACTCTATGTTCGTAGGAAAGGATGCCAGCAGGGCCTTGGCTCTCTTGTCTTTCAAACCTGAAGACATCAATGGTAATATCAAAGGTCTGAGTGAAGAAGAGCTTGTAATCTTGCAGGACTGGGAATACAAGTTCATGGAAAAGTATGTAAAAGTTGGGGAGCTTGTTCCAGAGGGGGGATTGAATGAGCATTCTGAAAATGGACACCAAAATTCCGAAACCGTGCGAGAAGAAGAACACGACAAAGATCAGTAA

Protein sequence

MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPSNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPEGGLNEHSENGHQNSETVREEEHDKDQ
Homology
BLAST of HG10019892 vs. NCBI nr
Match: XP_038903009.1 (membrane steroid-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 374.8 bits (961), Expect = 4.8e-100
Identity = 194/206 (94.17%), Postives = 197/206 (95.63%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           ME +WKAMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS
Sbjct: 1   MELYWKAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
           NSNLFVNDSVAD SQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG
Sbjct: 61  NSNLFVNDSVADPSQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
           SPYSMFVGKDASRALALLSFKPEDINGNI+GLSEEELVILQDWEYKFMEKYVKVG LV E
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGVLVSE 180

Query: 181 GGLNEHSENGHQNSETVREEEHDKDQ 207
             LNE SENGHQNSE VR EEHDKDQ
Sbjct: 181 AELNEDSENGHQNSEAVR-EEHDKDQ 205

BLAST of HG10019892 vs. NCBI nr
Match: XP_004136607.1 (membrane steroid-binding protein 2 [Cucumis sativus] >KGN59383.1 hypothetical protein Csa_000920 [Cucumis sativus])

HSP 1 Score: 372.1 bits (954), Expect = 3.1e-99
Identity = 194/207 (93.72%), Postives = 199/207 (96.14%), Query Frame = 0

Query: 1   MEFFWK-AMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNP 60
           MEF+WK AMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTV ISSSN 
Sbjct: 1   MEFYWKSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60

Query: 61  SNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADAS AVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP
Sbjct: 61  SNSNLFVNDSGADASLAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNI+GL+EEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGGLNEHSENGHQNSETVREEEHDKDQ 207
           EG LNEHSENGHQNSET  +EEHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETT-QEEHDKDQ 206

BLAST of HG10019892 vs. NCBI nr
Match: XP_008443179.1 (PREDICTED: membrane steroid-binding protein 2-like [Cucumis melo] >ADN33977.1 steroid binding protein [Cucumis melo subsp. melo] >KAA0053933.1 membrane steroid-binding protein 2-like [Cucumis melo var. makuwa] >TYK25471.1 membrane steroid-binding protein 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 372.1 bits (954), Expect = 3.1e-99
Identity = 193/207 (93.24%), Postives = 199/207 (96.14%), Query Frame = 0

Query: 1   MEFFWK-AMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNP 60
           MEF+W+ AMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSN 
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  SNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADASQAV VGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGR+FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNI+GLSEEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGGLNEHSENGHQNSETVREEEHDKDQ 207
           EG LNEHSENGHQNSET  + EHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETT-QVEHDKDQ 206

BLAST of HG10019892 vs. NCBI nr
Match: XP_022971619.1 (membrane steroid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 359.0 bits (920), Expect = 2.7e-95
Identity = 181/206 (87.86%), Postives = 196/206 (95.15%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           ME FW+AMEQI WY+GLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSN +
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
           NSNLFVND+VADASQ+VQVGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGPG
Sbjct: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
           SPYSMFVGKDASRALALLSFKPEDINGN++GL+EEELVILQDWEYKFMEKYVKVGELV E
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 GGLNEHSENGHQNSETVREEEHDKDQ 207
             LNE SENGHQ+++T REEE +KDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

BLAST of HG10019892 vs. NCBI nr
Match: KAG7027737.1 (Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 358.2 bits (918), Expect = 4.7e-95
Identity = 181/206 (87.86%), Postives = 196/206 (95.15%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           ME FW+AMEQI WY+GLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSN +
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
           NSNLFV+DSVADASQ+VQVGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGPG
Sbjct: 61  NSNLFVDDSVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
           SPYSMFVGKDASRALALLSFKPEDINGN++GL+EEELVILQDWEYKFMEKYVKVGELV E
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 GGLNEHSENGHQNSETVREEEHDKDQ 207
             LNE SENGHQ+++T REEE +KDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

BLAST of HG10019892 vs. ExPASy Swiss-Prot
Match: Q9M2Z4 (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.9e-44
Identity = 97/184 (52.72%), Postives = 125/184 (67.93%), Query Frame = 0

Query: 5   WKAM-EQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPSNSN 64
           W+ + E IT Y+GLSP AFFT+LA    V+Q+VS  FVSP E ++P ++ +    P +  
Sbjct: 6   WETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSP-EVHRPRSLEV---QPQSEP 65

Query: 65  LFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
           L            VQ+G +TE++L+ Y+GSD  KPLLMAIKGQIYDVS  RMFYGPG PY
Sbjct: 66  L---------PPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPEGGL 184
           ++F GKDASRALA +SF+ +D+ G+I GL   EL  LQDWEYKFM KYVKVG +  + G 
Sbjct: 126 ALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGE 176

Query: 185 NEHS 188
            + S
Sbjct: 186 GKES 176

BLAST of HG10019892 vs. ExPASy Swiss-Prot
Match: Q9FVZ7 (Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP1 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.1e-42
Identity = 92/172 (53.49%), Postives = 115/172 (66.86%), Query Frame = 0

Query: 5   WKAMEQ-ITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPSNSN 64
           W+ ++Q I  Y+GLSP AFFT +AA   ++ +VS +F  P     PP  P     P    
Sbjct: 9   WETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGP----PPPPPPRPRDEPEAEP 68

Query: 65  LFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
           L            VQ+G ++E++LR Y+GSDP KPLLMAIKGQIYDV+  RMFYGPG PY
Sbjct: 69  L---------PPPVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPY 128

Query: 125 SMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVG 176
           ++F GKDASRALA +SF+P+D+ G+I GL   EL  LQDWEYKFM KYVKVG
Sbjct: 129 ALFAGKDASRALAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVG 167

BLAST of HG10019892 vs. ExPASy Swiss-Prot
Match: Q9XFM6 (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 161.0 bits (406), Expect = 1.4e-38
Identity = 89/202 (44.06%), Postives = 120/202 (59.41%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           +E +    E I  Y+GLSP  FFT LA    ++Q++S  F SP +           +   
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD---------DVNRHQ 62

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
            +     +      Q VQVG +TE++L+ Y+GSDP KPLLMAIK QIYDV+  RMFYGPG
Sbjct: 63  RARSLAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
            PY++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG  V  
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG-TVKV 182

Query: 181 GGLNEHSENGHQNSETVREEEH 203
            G    + +  + +E V ++ H
Sbjct: 183 AGSEPETASVSEPTENVEQDAH 194

BLAST of HG10019892 vs. ExPASy Swiss-Prot
Match: Q9FVZ9 (Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP2 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.1e-35
Identity = 83/169 (49.11%), Postives = 104/169 (61.54%), Query Frame = 0

Query: 18  SPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPSNSNLFVNDSVADASQAV 77
           SP A    +A    V+++ SS+   P    +P   P   + P               + V
Sbjct: 70  SPVAVIAAVAGAAVVYKVGSSLLAPPPPPARPREEPSEEAPP-------------PPEPV 129

Query: 78  QVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALAL 137
           QVG +T ++L  Y+GSDP KPLLMAIKGQIYDVS  R+FYGPG PY++F GKDASRALA 
Sbjct: 130 QVGEITAEELLQYDGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRALAK 189

Query: 138 LSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGE----LVPEGG 183
           +SF+P+D+  +I GLS  EL  LQDWEYKF  KYVKVG     LV +GG
Sbjct: 190 MSFEPQDLTDDISGLSLLELSALQDWEYKFSSKYVKVGTIKKVLVEQGG 225

BLAST of HG10019892 vs. ExPASy Swiss-Prot
Match: Q9SK39 (Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.3e-23
Identity = 50/96 (52.08%), Postives = 71/96 (73.96%), Query Frame = 0

Query: 83  TEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALALLSFKP 142
           T +QL  YNG+D +KP+ +AIKG+++DV++G+ FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 143 EDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELV 179
           ED++ +++GL+E+E+  L DWE KF  KY  VG +V
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVV 99

BLAST of HG10019892 vs. ExPASy TrEMBL
Match: A0A5A7UDA3 (Membrane steroid-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006170 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.5e-99
Identity = 193/207 (93.24%), Postives = 199/207 (96.14%), Query Frame = 0

Query: 1   MEFFWK-AMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNP 60
           MEF+W+ AMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSN 
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  SNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADASQAV VGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGR+FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNI+GLSEEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGGLNEHSENGHQNSETVREEEHDKDQ 207
           EG LNEHSENGHQNSET  + EHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETT-QVEHDKDQ 206

BLAST of HG10019892 vs. ExPASy TrEMBL
Match: E5GBX8 (Steroid binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.5e-99
Identity = 193/207 (93.24%), Postives = 199/207 (96.14%), Query Frame = 0

Query: 1   MEFFWK-AMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNP 60
           MEF+W+ AMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSN 
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  SNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADASQAV VGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGR+FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNI+GLSEEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGGLNEHSENGHQNSETVREEEHDKDQ 207
           EG LNEHSENGHQNSET  + EHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETT-QVEHDKDQ 206

BLAST of HG10019892 vs. ExPASy TrEMBL
Match: A0A0A0LC44 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G815470 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.5e-99
Identity = 194/207 (93.72%), Postives = 199/207 (96.14%), Query Frame = 0

Query: 1   MEFFWK-AMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNP 60
           MEF+WK AMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTV ISSSN 
Sbjct: 1   MEFYWKSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVAISSSNS 60

Query: 61  SNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADAS AVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP
Sbjct: 61  SNSNLFVNDSGADASLAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNI+GL+EEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLNEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGGLNEHSENGHQNSETVREEEHDKDQ 207
           EG LNEHSENGHQNSET  +EEHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETT-QEEHDKDQ 206

BLAST of HG10019892 vs. ExPASy TrEMBL
Match: A0A1S3B7E4 (membrane steroid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103486840 PE=4 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.5e-99
Identity = 193/207 (93.24%), Postives = 199/207 (96.14%), Query Frame = 0

Query: 1   MEFFWK-AMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNP 60
           MEF+W+ AMEQITWY+GLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSN 
Sbjct: 1   MEFYWRSAMEQITWYTGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNS 60

Query: 61  SNSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGP 120
           SNSNLFVNDS ADASQAV VGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGR+FYGP
Sbjct: 61  SNSNLFVNDSAADASQAVLVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRIFYGP 120

Query: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVP 180
           GSPYSMFVGKDASRALALLSFKPEDINGNI+GLSEEELVILQDWEYKFMEKYVKVGELVP
Sbjct: 121 GSPYSMFVGKDASRALALLSFKPEDINGNIEGLSEEELVILQDWEYKFMEKYVKVGELVP 180

Query: 181 EGGLNEHSENGHQNSETVREEEHDKDQ 207
           EG LNEHSENGHQNSET  + EHDKDQ
Sbjct: 181 EGELNEHSENGHQNSETT-QVEHDKDQ 206

BLAST of HG10019892 vs. ExPASy TrEMBL
Match: A0A6J1I932 (membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470292 PE=4 SV=1)

HSP 1 Score: 359.0 bits (920), Expect = 1.3e-95
Identity = 181/206 (87.86%), Postives = 196/206 (95.15%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           ME FW+AMEQI WY+GLSPTAFFTILA+MIFVFQMVSSMFVSPEEFNKPPTVP+SSSN +
Sbjct: 1   MEIFWRAMEQINWYTGLSPTAFFTILASMIFVFQMVSSMFVSPEEFNKPPTVPVSSSNSA 60

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
           NSNLFVND+VADASQ+VQVGRLTEQQLRAY+GSDPNKPLLMAIKGQIYDVSSGR FYGPG
Sbjct: 61  NSNLFVNDTVADASQSVQVGRLTEQQLRAYDGSDPNKPLLMAIKGQIYDVSSGRTFYGPG 120

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
           SPYSMFVGKDASRALALLSFKPEDINGN++GL+EEELVILQDWEYKFMEKYVKVGELV E
Sbjct: 121 SPYSMFVGKDASRALALLSFKPEDINGNLEGLNEEELVILQDWEYKFMEKYVKVGELVSE 180

Query: 181 GGLNEHSENGHQNSETVREEEHDKDQ 207
             LNE SENGHQ+++T REEE +KDQ
Sbjct: 181 EALNERSENGHQSTDTNREEEPNKDQ 206

BLAST of HG10019892 vs. TAIR 10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3 )

HSP 1 Score: 179.5 bits (454), Expect = 2.8e-45
Identity = 97/184 (52.72%), Postives = 125/184 (67.93%), Query Frame = 0

Query: 5   WKAM-EQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPSNSN 64
           W+ + E IT Y+GLSP AFFT+LA    V+Q+VS  FVSP E ++P ++ +    P +  
Sbjct: 6   WETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSP-EVHRPRSLEV---QPQSEP 65

Query: 65  LFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPY 124
           L            VQ+G +TE++L+ Y+GSD  KPLLMAIKGQIYDVS  RMFYGPG PY
Sbjct: 66  L---------PPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPY 125

Query: 125 SMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPEGGL 184
           ++F GKDASRALA +SF+ +D+ G+I GL   EL  LQDWEYKFM KYVKVG +  + G 
Sbjct: 126 ALFAGKDASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGE 176

Query: 185 NEHS 188
            + S
Sbjct: 186 GKES 176

BLAST of HG10019892 vs. TAIR 10
Match: AT5G52240.2 (membrane steroid binding protein 1 )

HSP 1 Score: 162.2 bits (409), Expect = 4.6e-40
Identity = 85/181 (46.96%), Postives = 111/181 (61.33%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           +E +    E I  Y+GLSP  FFT LA    ++Q++S  F SP +           +   
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD---------DVNRHQ 62

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
            +     +      Q VQVG +TE++L+ Y+GSDP KPLLMAIK QIYDV+  RMFYGPG
Sbjct: 63  RARSLAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
            PY++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG +   
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVA 174

Query: 181 G 182
           G
Sbjct: 183 G 174

BLAST of HG10019892 vs. TAIR 10
Match: AT5G52240.1 (membrane steroid binding protein 1 )

HSP 1 Score: 161.0 bits (406), Expect = 1.0e-39
Identity = 89/202 (44.06%), Postives = 120/202 (59.41%), Query Frame = 0

Query: 1   MEFFWKAMEQITWYSGLSPTAFFTILAAMIFVFQMVSSMFVSPEEFNKPPTVPISSSNPS 60
           +E +    E I  Y+GLSP  FFT LA    ++Q++S  F SP +           +   
Sbjct: 3   LELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFD---------DVNRHQ 62

Query: 61  NSNLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPG 120
            +     +      Q VQVG +TE++L+ Y+GSDP KPLLMAIK QIYDV+  RMFYGPG
Sbjct: 63  RARSLAQEEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPG 122

Query: 121 SPYSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELVPE 180
            PY++F GKDASRALA +SF+ +D+  ++ GL   EL  LQDWEYKFM KY KVG  V  
Sbjct: 123 GPYALFAGKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVG-TVKV 182

Query: 181 GGLNEHSENGHQNSETVREEEH 203
            G    + +  + +E V ++ H
Sbjct: 183 AGSEPETASVSEPTENVEQDAH 194

BLAST of HG10019892 vs. TAIR 10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2 )

HSP 1 Score: 111.3 bits (277), Expect = 9.3e-25
Identity = 50/96 (52.08%), Postives = 71/96 (73.96%), Query Frame = 0

Query: 83  TEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSPYSMFVGKDASRALALLSFKP 142
           T +QL  YNG+D +KP+ +AIKG+++DV++G+ FYG G  YSMF GKDASRAL  +S   
Sbjct: 4   TAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSKNE 63

Query: 143 EDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELV 179
           ED++ +++GL+E+E+  L DWE KF  KY  VG +V
Sbjct: 64  EDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVV 99

BLAST of HG10019892 vs. TAIR 10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4 )

HSP 1 Score: 81.6 bits (200), Expect = 7.9e-16
Identity = 38/116 (32.76%), Postives = 62/116 (53.45%), Query Frame = 0

Query: 63  NLFVNDSVADASQAVQVGRLTEQQLRAYNGSDPNKPLLMAIKGQIYDVSSGRMFYGPGSP 122
           +L+   S        Q    + ++L  YNG+D   P+L+ I G ++DV+ G+  YG G  
Sbjct: 23  SLYFRSSFKSPQHQYQKRLFSAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGG 82

Query: 123 YSMFVGKDASRALALLSFKPEDINGNIKGLSEEELVILQDWEYKFMEKYVKVGELV 179
           Y+ F G+DASRA    +F  + +  +++GLS  E+  + DW   +   Y  VG+LV
Sbjct: 83  YNHFAGRDASRAFVSGNFTGDGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKLV 138

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038903009.14.8e-10094.17membrane steroid-binding protein 2-like [Benincasa hispida][more]
XP_004136607.13.1e-9993.72membrane steroid-binding protein 2 [Cucumis sativus] >KGN59383.1 hypothetical pr... [more]
XP_008443179.13.1e-9993.24PREDICTED: membrane steroid-binding protein 2-like [Cucumis melo] >ADN33977.1 st... [more]
XP_022971619.12.7e-9587.86membrane steroid-binding protein 2-like [Cucurbita maxima][more]
KAG7027737.14.7e-9587.86Membrane steroid-binding protein 2, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
Q9M2Z43.9e-4452.72Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1... [more]
Q9FVZ72.1e-4253.49Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q9XFM61.4e-3844.06Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1... [more]
Q9FVZ91.1e-3549.11Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M... [more]
Q9SK391.3e-2352.08Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A5A7UDA31.5e-9993.24Membrane steroid-binding protein 2-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
E5GBX81.5e-9993.24Steroid binding protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
A0A0A0LC441.5e-9993.72Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ... [more]
A0A1S3B7E41.5e-9993.24membrane steroid-binding protein 2-like OS=Cucumis melo OX=3656 GN=LOC103486840 ... [more]
A0A6J1I9321.3e-9587.86membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470... [more]
Match NameE-valueIdentityDescription
AT3G48890.12.8e-4552.72membrane-associated progesterone binding protein 3 [more]
AT5G52240.24.6e-4046.96membrane steroid binding protein 1 [more]
AT5G52240.11.0e-3944.06membrane steroid binding protein 1 [more]
AT2G24940.19.3e-2552.08membrane-associated progesterone binding protein 2 [more]
AT4G14965.17.9e-1632.76membrane-associated progesterone binding protein 4 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001199Cytochrome b5-like heme/steroid binding domainSMARTSM01117Cyt_b5_2coord: 82..178
e-value: 7.0E-18
score: 75.4
IPR001199Cytochrome b5-like heme/steroid binding domainPFAMPF00173Cyt-b5coord: 83..177
e-value: 7.9E-14
score: 51.6
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilyGENE3D3.10.120.10coord: 80..179
e-value: 1.7E-35
score: 123.3
IPR036400Cytochrome b5-like heme/steroid binding domain superfamilySUPERFAMILY55856Cytochrome b5-like heme/steroid binding domaincoord: 80..178
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 191..206
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..206
NoneNo IPR availablePANTHERPTHR10281MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATEDcoord: 6..200
NoneNo IPR availablePANTHERPTHR10281:SF45MEMBRANE STEROID-BINDING PROTEIN 2coord: 6..200

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10019892.1HG10019892.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane