HG10019836 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019836
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Chr04: 25996193 .. 25996708 (+)
RNA-Seq Expression	HG10019836
Synteny	HG10019836

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCAATGTGGGATTCTAAACTAGTAAATGAAGAAGAAAATCCATGGAAGGAAGCCAAAAACAGAGGCCATGGAGTGAAAGGGGTTTCCGAATTGCGTCTCAAAGCACCTCTCAAAGCATTCATTCAGCCTCCAGAAGAGAGATTGAGCTTGACGATCAGAGCCTCGCCGGCATCATCGGGAGAAATAATACCAATAATCGACATGTCGAAAACAGAGGCGGCAGAGTTAATATGCAAGGCGGCGGAGAAATGGGGTTTTTTCCAGGTGGTGAACCATGGAGTTCCGGCGGCGTTGCTTGGGAATGTAATGCAGGCAGCTAAGAGGTTCTTTGATCAGCCGGCGGAGGAGAAGAGGAAGTTGTTGAAGAAGAATTCGGGTTGTCGGAATGTGGTTTATGGGACCAGCTTTTTCTCTGAGGCAGAGAAAGCTTTGGAGTGGAAAGATTACTTGAGTATGCATTTTGTTTCTGAGGATGAAGCTTCTGCTTTCTGGCCTGCCGTTTCCAAGTAA

mRNA sequence

ATGGCTTCAATGTGGGATTCTAAACTAGTAAATGAAGAAGAAAATCCATGGAAGGAAGCCAAAAACAGAGGCCATGGAGTGAAAGGGGTTTCCGAATTGCGTCTCAAAGCACCTCTCAAAGCATTCATTCAGCCTCCAGAAGAGAGATTGAGCTTGACGATCAGAGCCTCGCCGGCATCATCGGGAGAAATAATACCAATAATCGACATGTCGAAAACAGAGGCGGCAGAGTTAATATGCAAGGCGGCGGAGAAATGGGGTTTTTTCCAGGTGGTGAACCATGGAGTTCCGGCGGCGTTGCTTGGGAATGTAATGCAGGCAGCTAAGAGGTTCTTTGATCAGCCGGCGGAGGAGAAGAGGAAGTTGTTGAAGAAGAATTCGGGTTGTCGGAATGTGGTTTATGGGACCAGCTTTTTCTCTGAGGCAGAGAAAGCTTTGGAGTGGAAAGATTACTTGAGTATGCATTTTGTTTCTGAGGATGAAGCTTCTGCTTTCTGGCCTGCCGTTTCCAAGTAA

Coding sequence (CDS)

ATGGCTTCAATGTGGGATTCTAAACTAGTAAATGAAGAAGAAAATCCATGGAAGGAAGCCAAAAACAGAGGCCATGGAGTGAAAGGGGTTTCCGAATTGCGTCTCAAAGCACCTCTCAAAGCATTCATTCAGCCTCCAGAAGAGAGATTGAGCTTGACGATCAGAGCCTCGCCGGCATCATCGGGAGAAATAATACCAATAATCGACATGTCGAAAACAGAGGCGGCAGAGTTAATATGCAAGGCGGCGGAGAAATGGGGTTTTTTCCAGGTGGTGAACCATGGAGTTCCGGCGGCGTTGCTTGGGAATGTAATGCAGGCAGCTAAGAGGTTCTTTGATCAGCCGGCGGAGGAGAAGAGGAAGTTGTTGAAGAAGAATTCGGGTTGTCGGAATGTGGTTTATGGGACCAGCTTTTTCTCTGAGGCAGAGAAAGCTTTGGAGTGGAAAGATTACTTGAGTATGCATTTTGTTTCTGAGGATGAAGCTTCTGCTTTCTGGCCTGCCGTTTCCAAGTAA

Protein sequence

MASMWDSKLVNEEENPWKEAKNRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTEAAELICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSFFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK

Homology

[Close]

BLAST of HG10019836 vs. NCBI nr
Match: XP_011652189.1 (feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical protein Csa_001767 [Cucumis sativus])

HSP 1 Score: 241.5 bits (615), Expect = 5.3e-60
Identity = 121/176 (68.75%), Postives = 145/176 (82.39%), Query Frame = 0


Query: 1   MASMW----DSKLVNEE-ENPWKEAKNRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIR 60
           MASM+    DSK VNEE ++PWKEA N+G+G  G+S++RL+A  KAFIQPPE+R S  IR
Sbjct: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60

Query: 61  ASPASSGEIIPIIDMSKTEAAELICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQP 120
           AS  +SG+ IPIIDMSK EAAELIC+AAEKWGFFQV+NHGVPA L+ +VM AA+RF  Q 
Sbjct: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120

Query: 121 AEEKRKLLKKNSGCRNVVYGTSFFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
           AEEKR+ LK+N+ C NVVY TSFF+EAEK LEW DYLSM+FVSE+EA AFWPA+SK
Sbjct: 121 AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISK 176

[Close]

BLAST of HG10019836 vs. NCBI nr
Match: RVW48634.1 (Feruloyl CoA ortho-hydroxylase 1 [Vitis vinifera])

HSP 1 Score: 178.3 bits (451), Expect = 5.5e-41
Identity = 91/153 (59.48%), Postives = 112/153 (73.20%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAEL 81
           N+GHGVKG+SE++L +  K FIQPPEER    I AS  +  E IP+IDM+  +    AEL
Sbjct: 21  NQGHGVKGLSEMKLPSIPKQFIQPPEER----IDASKVAREECIPVIDMAGVDDPKVAEL 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEK+K  K+NS   +V +GTSF
Sbjct: 81  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKKYSKENSNSNSVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             +AEK+LEWKDYLS+ +VSEDEASA WP+V K
Sbjct: 141 TPQAEKSLEWKDYLSLFYVSEDEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. NCBI nr
Match: XP_034688352.1 (feruloyl CoA ortho-hydroxylase F6H1-3-like [Vitis riparia])

HSP 1 Score: 176.4 bits (446), Expect = 2.1e-40
Identity = 91/153 (59.48%), Postives = 111/153 (72.55%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMS---KTEAAEL 81
           N+GHGVKG+SE++L +  K FIQPPEER    I AS     E IP+IDM+     + AEL
Sbjct: 21  NQGHGVKGLSEMKLPSLPKHFIQPPEER----IDASKVVCEECIPVIDMAGLDDPKVAEL 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEK+K  K+NS   +V +GTSF
Sbjct: 81  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKKYSKENSNSNSVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             +AEK+LEWKDYLS+ +VSEDEASA WP+V K
Sbjct: 141 TPQAEKSLEWKDYLSLFYVSEDEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. NCBI nr
Match: RVX12880.1 (Feruloyl CoA ortho-hydroxylase 1 [Vitis vinifera])

HSP 1 Score: 176.4 bits (446), Expect = 2.1e-40
Identity = 90/153 (58.82%), Postives = 111/153 (72.55%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAEL 81
           N+GHGVKG+SE++L +  K FIQPPEER    I AS  +  E IP+IDM+  +    AEL
Sbjct: 21  NQGHGVKGLSEMKLPSIPKQFIQPPEER----IDASKVAREECIPVIDMAGVDDPKVAEL 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEK+   K+NS   +V +GTSF
Sbjct: 81  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKNYSKENSNSNSVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             +AEK+LEWKDYLS+ +VSEDEASA WP+V K
Sbjct: 141 TPQAEKSLEWKDYLSLFYVSEDEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. NCBI nr
Match: XP_002285699.1 (PREDICTED: feruloyl CoA ortho-hydroxylase 1 [Vitis vinifera])

HSP 1 Score: 176.0 bits (445), Expect = 2.7e-40
Identity = 94/170 (55.29%), Postives = 116/170 (68.24%), Query Frame = 0


Query: 8   KLVNEEENPWKEAK---NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEI 67
           K+V    N W       N+GHGVKG+SE++L +  K FIQPPEER    I AS     E 
Sbjct: 4   KMVQSATNSWDITDFVVNQGHGVKGLSEMKLLSIPKQFIQPPEER----IDASKVVCEEC 63

Query: 68  IPIIDMS---KTEAAELICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRK 127
           IP+IDM+     + A++IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEKRK
Sbjct: 64  IPVIDMACLDGPKVAQMICDAAEKWGFFQVVNHGVPLGVLEDVKEATHRFFALPGEEKRK 123

Query: 128 LLKKNSGCRNVVYGTSFFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             K+NS   NV +GTSF  +AEK+LEWKDYLS+ +VSE+EASA WP+V K
Sbjct: 124 YSKENSRSNNVRFGTSFTPQAEKSLEWKDYLSLFYVSENEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. ExPASy Swiss-Prot
Match: Q9LHN8 (Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.1e-36
Identity = 81/152 (53.29%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 23  RGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAELI 82
           +G+GVKG+SE  +KA  + +IQP EERL   I      + E IP+IDMS  +    AE +
Sbjct: 23  KGNGVKGLSETGIKALPEQYIQPLEERL---INKFVNETDEAIPVIDMSNPDEDRVAEAV 82

Query: 83  CKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSFF 142
           C AAEKWGFFQV+NHGVP  +L +V  A  +FF+ P EEKRK  K+NS    V +GTSF 
Sbjct: 83  CDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTSFS 142

Query: 143 SEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             AE+ALEWKDYLS+ FVSE EA  FWP + +
Sbjct: 143 PLAEQALEWKDYLSLFFVSEAEAEQFWPDICR 171

[Close]

BLAST of HG10019836 vs. ExPASy Swiss-Prot
Match: W5QJZ5 (Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox4 OS=Ruta graveolens OX=37565 GN=DIOX4 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.5e-36
Identity = 81/152 (53.29%), Postives = 100/152 (65.79%), Query Frame = 0


Query: 23  RGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTEAAEL---I 82
           +GHGVKG+SEL +K   K F QP EER S       AS    IP+IDMSK ++ E+   I
Sbjct: 26  KGHGVKGLSELGIKTLPKQFHQPLEERFSEKKILERAS----IPLIDMSKWDSPEVVKSI 85

Query: 83  CKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSFF 142
           C AAE WGFFQ+VNHGVP   L  V +A  RFF  PAEEK K  K+NS   NV +G+SF 
Sbjct: 86  CDAAEHWGFFQIVNHGVPLETLQRVKEATHRFFALPAEEKNKYSKENSPINNVRFGSSFV 145

Query: 143 SEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
              EKALEWKD+LSM +VSE+E + +WP + +
Sbjct: 146 PHVEKALEWKDFLSMFYVSEEETNTYWPPICR 173

[Close]

BLAST of HG10019836 vs. ExPASy Swiss-Prot
Match: G9M9M1 (Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.2e-36
Identity = 77/154 (50.00%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSK----TEAAE 81
           + G+GVKG++++ L+A  K ++QP EER    I  S     + IP+ID+S+     +  +
Sbjct: 18  HEGNGVKGLADMGLEALPKQYVQPEEER----ITTSTVIVDDTIPVIDLSEWGSDPKVGD 77

Query: 82  LICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTS 141
           +IC+AAEKWGFFQ+VNHGVP  +L  V  A  RFF  PAEEK K  K NS   NV YGTS
Sbjct: 78  MICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHCKDNSPSNNVRYGTS 137

Query: 142 FFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
           F   AEKALEWKD+LS+ +VS++EA+A WP+  +
Sbjct: 138 FTPHAEKALEWKDFLSLFYVSDEEAAALWPSACR 167

[Close]

BLAST of HG10019836 vs. ExPASy Swiss-Prot
Match: G9M9M2 (Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 5.5e-36
Identity = 78/154 (50.65%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSK----TEAAE 81
           + G+GVKG++++ L++  K +IQP EER    I  S     + IP+ID+S+     +  +
Sbjct: 18  HEGNGVKGLADMGLQSLPKQYIQPAEER----ITTSTVIVDDTIPVIDLSEWGSDPKVGD 77

Query: 82  LICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTS 141
           LIC+AAEKWGFFQ+VNHGVP  +L  V  A  RFF  PAEEK K  K NS   NV YGTS
Sbjct: 78  LICEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSPSNNVRYGTS 137

Query: 142 FFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
           F   AEKALEWKD+LS+ +VS++EA+A WP+  +
Sbjct: 138 FTPHAEKALEWKDFLSLFYVSDEEAAALWPSACR 167

[Close]

BLAST of HG10019836 vs. ExPASy Swiss-Prot
Match: G9M9M0 (Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.6e-35
Identity = 76/154 (49.35%), Postives = 103/154 (66.88%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSK----TEAAE 81
           + G+GVKG++++ L+A  K ++QP EER    I  S     + IP+ID+S+     +  +
Sbjct: 18  HEGNGVKGLADMGLEALPKQYVQPEEER----ITTSTVIVDDTIPVIDLSEWGSDPKVGD 77

Query: 82  LICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTS 141
           + C+AAEKWGFFQ+VNHGVP  +L  V  A  RFF  PAEEK K  K NS   NV YGTS
Sbjct: 78  MNCEAAEKWGFFQIVNHGVPLEVLEEVKAATYRFFRLPAEEKNKHSKDNSPSNNVRYGTS 137

Query: 142 FFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
           F   AEKALEWKD+LS+ +VS++EA+A WP+  +
Sbjct: 138 FTPHAEKALEWKDFLSLFYVSDEEAAALWPSACR 167

[Close]

BLAST of HG10019836 vs. ExPASy TrEMBL
Match: A0A0A0LFH9 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G821020 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.6e-60
Identity = 121/176 (68.75%), Postives = 145/176 (82.39%), Query Frame = 0


Query: 1   MASMW----DSKLVNEE-ENPWKEAKNRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIR 60
           MASM+    DSK VNEE ++PWKEA N+G+G  G+S++RL+A  KAFIQPPE+R S  IR
Sbjct: 1   MASMYDSDSDSKPVNEEGDHPWKEAINKGYGTTGLSKMRLRALPKAFIQPPEKRSSSMIR 60

Query: 61  ASPASSGEIIPIIDMSKTEAAELICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQP 120
           AS  +SG+ IPIIDMSK EAAELIC+AAEKWGFFQV+NHGVPA L+ +VM AA+RF  Q 
Sbjct: 61  ASSQASGDTIPIIDMSKAEAAELICEAAEKWGFFQVINHGVPAVLMSDVMHAARRFLGQA 120

Query: 121 AEEKRKLLKKNSGCRNVVYGTSFFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
           AEEKR+ LK+N+ C NVVY TSFF+EAEK LEW DYLSM+FVSE+EA AFWPA+SK
Sbjct: 121 AEEKRRFLKENTSCSNVVYMTSFFAEAEKGLEWSDYLSMNFVSEEEAYAFWPAISK 176

[Close]

BLAST of HG10019836 vs. ExPASy TrEMBL
Match: A0A438ELM9 (Feruloyl CoA ortho-hydroxylase 1 OS=Vitis vinifera OX=29760 GN=F6'H1_2 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.7e-41
Identity = 91/153 (59.48%), Postives = 112/153 (73.20%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAEL 81
           N+GHGVKG+SE++L +  K FIQPPEER    I AS  +  E IP+IDM+  +    AEL
Sbjct: 21  NQGHGVKGLSEMKLPSIPKQFIQPPEER----IDASKVAREECIPVIDMAGVDDPKVAEL 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEK+K  K+NS   +V +GTSF
Sbjct: 81  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKKYSKENSNSNSVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             +AEK+LEWKDYLS+ +VSEDEASA WP+V K
Sbjct: 141 TPQAEKSLEWKDYLSLFYVSEDEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. ExPASy TrEMBL
Match: A0A438JV86 (Feruloyl CoA ortho-hydroxylase 1 OS=Vitis vinifera OX=29760 GN=F6'H1_3 PE=3 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 1.0e-40
Identity = 90/153 (58.82%), Postives = 111/153 (72.55%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAEL 81
           N+GHGVKG+SE++L +  K FIQPPEER    I AS  +  E IP+IDM+  +    AEL
Sbjct: 21  NQGHGVKGLSEMKLPSIPKQFIQPPEER----IDASKVAREECIPVIDMAGVDDPKVAEL 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEK+   K+NS   +V +GTSF
Sbjct: 81  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKNYSKENSNSNSVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             +AEK+LEWKDYLS+ +VSEDEASA WP+V K
Sbjct: 141 TPQAEKSLEWKDYLSLFYVSEDEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. ExPASy TrEMBL
Match: F6GUJ9 (Fe2OG dioxygenase domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_06s0004g00770 PE=3 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 1.3e-40
Identity = 94/170 (55.29%), Postives = 116/170 (68.24%), Query Frame = 0


Query: 8   KLVNEEENPWKEAK---NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEI 67
           K+V    N W       N+GHGVKG+SE++L +  K FIQPPEER    I AS     E 
Sbjct: 4   KMVQSATNSWDITDFVVNQGHGVKGLSEMKLLSIPKQFIQPPEER----IDASKVVCEEC 63

Query: 68  IPIIDMS---KTEAAELICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRK 127
           IP+IDM+     + A++IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEKRK
Sbjct: 64  IPVIDMACLDGPKVAQMICDAAEKWGFFQVVNHGVPLGVLEDVKEATHRFFALPGEEKRK 123

Query: 128 LLKKNSGCRNVVYGTSFFSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             K+NS   NV +GTSF  +AEK+LEWKDYLS+ +VSE+EASA WP+V K
Sbjct: 124 YSKENSRSNNVRFGTSFTPQAEKSLEWKDYLSLFYVSENEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. ExPASy TrEMBL
Match: F6GUK0 (Fe2OG dioxygenase domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_06s0004g00760 PE=3 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 2.3e-40
Identity = 90/153 (58.82%), Postives = 111/153 (72.55%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMS---KTEAAEL 81
           N+GHGVKG+SE++L +  K FIQPPEER    I AS     + IP+IDM+     + AEL
Sbjct: 21  NQGHGVKGLSEMKLPSLPKQFIQPPEER----IDASKVVCEKCIPVIDMAGLDDPKVAEL 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           IC AAEKWGFFQVVNHGVP  +L +V +A  RFF  P EEK+K  K+NS   +V +GTSF
Sbjct: 81  ICDAAEKWGFFQVVNHGVPIGVLEDVKEATHRFFALPGEEKKKYSKENSNSNSVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             +AEK+LEWKDYLS+ +VSEDEASA WP+V K
Sbjct: 141 TPQAEKSLEWKDYLSLFYVSEDEASALWPSVCK 169

[Close]

BLAST of HG10019836 vs. TAIR 10
Match: AT3G13610.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 7.9e-38
Identity = 81/152 (53.29%), Postives = 102/152 (67.11%), Query Frame = 0


Query: 23  RGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAELI 82
           +G+GVKG+SE  +KA  + +IQP EERL   I      + E IP+IDMS  +    AE +
Sbjct: 23  KGNGVKGLSETGIKALPEQYIQPLEERL---INKFVNETDEAIPVIDMSNPDEDRVAEAV 82

Query: 83  CKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSFF 142
           C AAEKWGFFQV+NHGVP  +L +V  A  +FF+ P EEKRK  K+NS    V +GTSF 
Sbjct: 83  CDAAEKWGFFQVINHGVPLEVLDDVKAATHKFFNLPVEEKRKFTKENSLSTTVRFGTSFS 142

Query: 143 SEAEKALEWKDYLSMHFVSEDEASAFWPAVSK 172
             AE+ALEWKDYLS+ FVSE EA  FWP + +
Sbjct: 143 PLAEQALEWKDYLSLFFVSEAEAEQFWPDICR 171

[Close]

BLAST of HG10019836 vs. TAIR 10
Match: AT1G55290.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 141.0 bits (354), Expect = 9.1e-34
Identity = 76/149 (51.01%), Postives = 96/149 (64.43%), Query Frame = 0


Query: 22  NRGHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSKTE---AAEL 81
           + G+GVKG+SE  +K     +IQP EERL +        S E IP+ID+S  +    ++ 
Sbjct: 21  HEGNGVKGLSETGIKVLPDQYIQPFEERL-INFHVK-EDSDESIPVIDISNLDEKSVSKA 80

Query: 82  ICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSF 141
           +C AAE+WGFFQV+NHGV   +L N+  A  RFF  P EEKRK  ++ S   NV +GTSF
Sbjct: 81  VCDAAEEWGFFQVINHGVSMEVLENMKTATHRFFGLPVEEKRKFSREKSLSTNVRFGTSF 140

Query: 142 FSEAEKALEWKDYLSMHFVSEDEASAFWP 168
              AEKALEWKDYLS+ FVSE EAS  WP
Sbjct: 141 SPHAEKALEWKDYLSLFFVSEAEASQLWP 167

[Close]

BLAST of HG10019836 vs. TAIR 10
Match: AT3G12900.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 107.8 bits (268), Expect = 8.5e-24
Identity = 65/150 (43.33%), Postives = 85/150 (56.67%), Query Frame = 0


Query: 24  GHGVKGVSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMSK------TEAAE 83
           G+GVKG+ +  L +  + F+QP  ER    I    A + E    ID+S        E A+
Sbjct: 19  GNGVKGMIDSGLSSVPRPFVQPLSER----IPTQKALTCEATQPIDLSNLDGPQHKEVAK 78

Query: 84  LICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTS 143
            I +AAE  GFFQVVNHGV   LL  +  +A  FF Q  EEK   LK+ S  + V YGTS
Sbjct: 79  QIVEAAETLGFFQVVNHGVSVELLELLKSSAHEFFAQAPEEKSMYLKEVSPSKLVKYGTS 138

Query: 144 FFSEAEKALEWKDYLSMHFVSEDEASAFWP 168
           F  + EKA+EWKDY+SM + ++ EA   WP
Sbjct: 139 FVPDKEKAIEWKDYVSMLYTNDSEALQHWP 164

[Close]

BLAST of HG10019836 vs. TAIR 10
Match: AT5G12270.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 95.1 bits (235), Expect = 5.7e-20
Identity = 58/155 (37.42%), Postives = 85/155 (54.84%), Query Frame = 0


Query: 22  NRGHGVKG-VSELRLKAPLKAFIQPPEERLSLTIRASPASSGEIIPIIDMS---KTEAAE 81
           N+ +GVKG V  L L      +IQPP+ER +    +     G  +P+ID+S   +   A 
Sbjct: 16  NQKNGVKGLVDFLTLTTLPSPYIQPPQERFT----SDKILLGSPVPVIDVSNWNEPHVAR 75

Query: 82  LICKAAEKWGFFQVVNHGVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTS 141
            IC AA K G FQ+VNHG+  A    V+ AA+ FF+ PAEE+R+  + +S        TS
Sbjct: 76  EICHAASKLGLFQIVNHGIAPAEFKGVIAAARGFFELPAEERRRYWRGSSVSETAWLTTS 135

Query: 142 FFSEAEKALEWKDYLSMHFVSE-DEASAFWPAVSK 172
           F    E  LEW+D+L   ++ +  + +A WP+V K
Sbjct: 136 FNPCIESVLEWRDFLKFEYLPQRHDFAATWPSVCK 166

[Close]

BLAST of HG10019836 vs. TAIR 10
Match: AT2G44800.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 4.7e-14
Identity = 42/134 (31.34%), Postives = 74/134 (55.22%), Query Frame = 0


Query: 42  FIQPPEERLSLTIRASPASSGEIIPIIDMS-------KTEAAELICKAAEKWGFFQVVNH 101
           ++ PP +R +L   +S  +S   +P+ID+S       ++ A   I  A +++GFFQV+NH
Sbjct: 31  YVLPPSQRPAL--GSSLGTSETTLPVIDLSLLHQPFLRSLAIHEISMACKEFGFFQVINH 90

Query: 102 GVPAALLGNVMQAAKRFFDQPAEEKRKLLKKNSGCRNVVYGTSFFSEAEKALEWKDYLSM 161
           G+P++++ + + AA +FFD P EEK  L+  N     V YGTS     ++   W+D++  
Sbjct: 91  GIPSSVVNDALDAATQFFDLPVEEKMLLVSANVH-EPVRYGTSLNHSTDRVHYWRDFIKH 150

Query: 162 HFVSEDEASAFWPA 169
           +     +    WP+
Sbjct: 151 YSHPLSKWIDMWPS 161

The following BLAST results are available for this feature:

BLAST of HG10019836 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652189.1	5.3e-60	68.75	feruloyl CoA ortho-hydroxylase 1 [Cucumis sativus] >KGN59447.1 hypothetical prot...	[more]
RVW48634.1	5.5e-41	59.48	Feruloyl CoA ortho-hydroxylase 1 [Vitis vinifera]	[more]
XP_034688352.1	2.1e-40	59.48	feruloyl CoA ortho-hydroxylase F6H1-3-like [Vitis riparia]	[more]
RVX12880.1	2.1e-40	58.82	Feruloyl CoA ortho-hydroxylase 1 [Vitis vinifera]	[more]
XP_002285699.1	2.7e-40	55.29	PREDICTED: feruloyl CoA ortho-hydroxylase 1 [Vitis vinifera]	[more]

BLAST of HG10019836 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LHN8	1.1e-36	53.29	Feruloyl CoA ortho-hydroxylase 1 OS=Arabidopsis thaliana OX=3702 GN=F6'H1 PE=1 S...	[more]
W5QJZ5	2.5e-36	53.29	Bi-functional coumaroyl CoA and feruloyl CoA ortho-hydroxylase Diox4 OS=Ruta gra...	[more]
G9M9M1	4.2e-36	50.00	Feruloyl CoA ortho-hydroxylase F6H1-2 OS=Ipomoea batatas OX=4120 GN=F6H1-2 PE=1 ...	[more]
G9M9M2	5.5e-36	50.65	Feruloyl CoA ortho-hydroxylase F6H1-3 OS=Ipomoea batatas OX=4120 GN=F6H1-3 PE=1 ...	[more]
G9M9M0	3.6e-35	49.35	Feruloyl CoA ortho-hydroxylase F6H1-1 OS=Ipomoea batatas OX=4120 GN=F6H1-1 PE=2 ...	[more]

BLAST of HG10019836 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LFH9	2.6e-60	68.75	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A438ELM9	2.7e-41	59.48	Feruloyl CoA ortho-hydroxylase 1 OS=Vitis vinifera OX=29760 GN=F6'H1_2 PE=3 SV=1	[more]
A0A438JV86	1.0e-40	58.82	Feruloyl CoA ortho-hydroxylase 1 OS=Vitis vinifera OX=29760 GN=F6'H1_3 PE=3 SV=1	[more]
F6GUJ9	1.3e-40	55.29	Fe2OG dioxygenase domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_06...	[more]
F6GUK0	2.3e-40	58.82	Fe2OG dioxygenase domain-containing protein OS=Vitis vinifera OX=29760 GN=VIT_06...	[more]

BLAST of HG10019836 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13610.1	7.9e-38	53.29	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT1G55290.1	9.1e-34	51.01	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G12900.1	8.5e-24	43.33	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G12270.1	5.7e-20	37.42	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT2G44800.1	4.7e-14	31.34	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 18..171 e-value: 2.2E-32 score: 114.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 65..164 e-value: 9.7E-23 score: 81.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..23
None	No IPR available	PANTHER	PTHR10209	OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN	coord: 22..168
None	No IPR available	PANTHER	PTHR10209:SF770	FERULOYL COA ORTHO-HYDROXYLASE 1-LIKE	coord: 22..168
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 26..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10019836.1	HG10019836.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity