HG10019715 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019715
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	ribonuclease MC-like
Location	Chr04: 24795986 .. 24797427 (-)
RNA-Seq Expression	HG10019715
Synteny	HG10019715

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAGGAGAGAAATTATATTGGCCTTGAGTATATTGGCATTCATAGCAAGGTGTGAGGGAGTTAACGAGTATGACTACTTGCGAGTTGTGTTTCAATGGCAGCCAGCCACTTGCGGTGATGCGACCAAGCCTTATCCTTATTGCAATCAAGCTCCACAAAATAGATTCAGTATCCATGGGATGTGGCCTAGCCTATACAATGGTCAAACCCAATGTTCTGGTAGTTCCTTCGATATAAACCAGGTAATTACATTATCACAATTATTATTTGTGAATCATAAATACAAGTTTGGAGAGTTGTGAACTCCTGAGGCTTACAGTGTATAAAGAATTAATAAGACTTAAAATTGATGTTTTTTTAGGCCTACAATGTATAACCGATGAGTTATACTCATATTGATACATCATATTAATTTTTGTAAACAAATTTTAACAATATTTTGACATATAGGCCCAATAGATCAATTCTTATAAACATGAACATTAAATTTCAACATTTTAGAAGATCAAAAATGAAATTGAAAAGTATATTATTATTATTTTTTTAGTTAAGCTATGCAGGGATTATCATTAATCAAATCTCAAACTGCTTTTAGATCCAAGTAATAGTGCCTTAACGAATTAAACTATATTCATGTTGACAATCGAAAATTACCAACGACCAGTTAGTTTTTAAATAAACACTACTTCCGACTATAATTTCTTTAATTTGTTATCTAGTTACGTAGGAATATATCATTTTAAGAAAACCAAACCAACTTTTGATAACTTAAAAAAAGTAGTATTTTTAAAAACGTGTTTTTTTAAATAAAAAATTTAGCTAAAAATTCAAGTATTCAATTAAGAAACATGAAAATTCTTTGTAAGAAAACAAACATAATTTTAAAAAATCAAAAATAAAAAAATCAAACGGTTATCAAATAGAGTCTATCCAATTATGAATGATGTTTCGGTTTTTCTAACCCTAAACCATTCCATTATAACTCTCTACATATTACTTTGTCTAGGTTAAGGTTCTAGAACCCAGACTGAGTGAAATGTGGGGAAACATTATCACAGGAGACAACCAATGGCTATGGAAACACGAATGGGATAAACATGGAACTTGCATAGAAGACATGTTATTAGATCAATCAGGATATTTTGAATATGCAATGAACACACTCTATTATTTCAATCCTGAGGAAATTCTAAAGAACAGTGGAATTTGGCCTACTCAAGAAGGATACTACACATTGGATGAAATTAAGAATGCAATCAGAGCAAAAACTGGAAAATTACCAGCCATTAGCTGCAATTACGATCATTGGACTTCATATGAACAATTGCATGAAATAAGCTACTGTACTACTCGGGATGGTTCAACTTTTGTGGATTGTCCCTCCAATGCCAACAAATGCAATGGTTATTCTGCCATGTGGTATCCCTCTCCCTACTAG

mRNA sequence

ATGGTGAGGAGAGAAATTATATTGGCCTTGAGTATATTGGCATTCATAGCAAGGTGTGAGGGAGTTAACGAGTATGACTACTTGCGAGTTGTGTTTCAATGGCAGCCAGCCACTTGCGGTGATGCGACCAAGCCTTATCCTTATTGCAATCAAGCTCCACAAAATAGATTCAGTATCCATGGGATGTGGCCTAGCCTATACAATGGTCAAACCCAATGTTCTGGTAGTTCCTTCGATATAAACCAGGTTAAGGTTCTAGAACCCAGACTGAGTGAAATGTGGGGAAACATTATCACAGGAGACAACCAATGGCTATGGAAACACGAATGGGATAAACATGGAACTTGCATAGAAGACATGTTATTAGATCAATCAGGATATTTTGAATATGCAATGAACACACTCTATTATTTCAATCCTGAGGAAATTCTAAAGAACAGTGGAATTTGGCCTACTCAAGAAGGATACTACACATTGGATGAAATTAAGAATGCAATCAGAGCAAAAACTGGAAAATTACCAGCCATTAGCTGCAATTACGATCATTGGACTTCATATGAACAATTGCATGAAATAAGCTACTGTACTACTCGGGATGGTTCAACTTTTGTGGATTGTCCCTCCAATGCCAACAAATGCAATGGTTATTCTGCCATGTGGTATCCCTCTCCCTACTAG

Coding sequence (CDS)

ATGGTGAGGAGAGAAATTATATTGGCCTTGAGTATATTGGCATTCATAGCAAGGTGTGAGGGAGTTAACGAGTATGACTACTTGCGAGTTGTGTTTCAATGGCAGCCAGCCACTTGCGGTGATGCGACCAAGCCTTATCCTTATTGCAATCAAGCTCCACAAAATAGATTCAGTATCCATGGGATGTGGCCTAGCCTATACAATGGTCAAACCCAATGTTCTGGTAGTTCCTTCGATATAAACCAGGTTAAGGTTCTAGAACCCAGACTGAGTGAAATGTGGGGAAACATTATCACAGGAGACAACCAATGGCTATGGAAACACGAATGGGATAAACATGGAACTTGCATAGAAGACATGTTATTAGATCAATCAGGATATTTTGAATATGCAATGAACACACTCTATTATTTCAATCCTGAGGAAATTCTAAAGAACAGTGGAATTTGGCCTACTCAAGAAGGATACTACACATTGGATGAAATTAAGAATGCAATCAGAGCAAAAACTGGAAAATTACCAGCCATTAGCTGCAATTACGATCATTGGACTTCATATGAACAATTGCATGAAATAAGCTACTGTACTACTCGGGATGGTTCAACTTTTGTGGATTGTCCCTCCAATGCCAACAAATGCAATGGTTATTCTGCCATGTGGTATCCCTCTCCCTACTAG

Protein sequence

MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIHGMWPSLYNGQTQCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWYPSPY

Homology

[Close]

BLAST of HG10019715 vs. NCBI nr
Match: XP_038904516.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 356.7 bits (914), Expect = 1.5e-94
Identity = 161/239 (67.36%), Postives = 180/239 (75.31%), Query Frame = 0


Query: 19  CEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIHGMWPSLYNGQTQCSGSSF 78
           CEGVNEYD+LRVVFQWQPATCG ATKPYPYC Q PQNRF   G+  ++     Q S S  
Sbjct: 2   CEGVNEYDFLRVVFQWQPATCGVATKPYPYCYQTPQNRF---GLDYTMAKPNVQASSSQV 61

Query: 79  DI--------------------------------NQVKVLEPRLSEMWGNIITGDNQWLW 138
           ++                                 +VKVLEPRL+EMWGN+ITGDNQWLW
Sbjct: 62  NLLFYPKAHFVQTSKQLTRARAHITLVLFKGRENYKVKVLEPRLNEMWGNVITGDNQWLW 121

Query: 139 KHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAI 198
           K EWDKHGTCIED+L DQSGYFEY MNTLYYFNPEEILKN GIWPTQEGYYTLDEIK+ I
Sbjct: 122 KPEWDKHGTCIEDILFDQSGYFEYTMNTLYYFNPEEILKNGGIWPTQEGYYTLDEIKSVI 181

Query: 199 RAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWYPSPY 226
           +A+TGKLPA+ CNY+HWTSY+QLHEISYC T++G TFVDCPSNANKCNG+SAMWYP PY
Sbjct: 182 KARTGKLPAVGCNYNHWTSYQQLHEISYCVTKEGQTFVDCPSNANKCNGHSAMWYPPPY 237

[Close]

BLAST of HG10019715 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 286.2 bits (731), Expect = 2.5e-73
Identity = 127/222 (57.21%), Postives = 161/222 (72.52%), Query Frame = 0


Query: 1   MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIH 60
           M +REI+L LS+L  IA CEG+NE+DYL+VV QWQPATC  + KPYP C Q P NR+SIH
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GMWPSLYNG-QTQCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIED 120
           G+WPSLY+G  TQCSGS F+ N + +L+P +SE+W NII G+N WLW HEWDKHGTC E+
Sbjct: 61  GVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEE 120

Query: 121 MLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCN 180
           +L DQ  YF +AM+T   +N   +L NS I P  +  Y+ D++  AIRAKTGK PA+ CN
Sbjct: 121 ILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQ-IYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 YDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWY 222
           Y+ WT  +Q+HE+S C   D S  VDCP NANKC+ Y  +WY
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNY-FVWY 220

[Close]

BLAST of HG10019715 vs. NCBI nr
Match: XP_038903744.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 275.8 bits (704), Expect = 3.3e-70
Identity = 128/224 (57.14%), Postives = 157/224 (70.09%), Query Frame = 0


Query: 1   MVRREIILALSILAFIAR-CEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSI 60
           M ++EI L LS+L  I   CEGVN+YDYL+VV QWQPATC D  KPYPYC Q P NRFSI
Sbjct: 1   MEKKEIGLILSMLVMIVSCCEGVNDYDYLQVVIQWQPATCTDWKKPYPYCFQDPDNRFSI 60

Query: 61  HGMWPSLYNG-QTQCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           HGMWPSLY+G QT C GS FD+NQ+ +L+P +SE+W N++TG NQWLW HEWDKHGTC E
Sbjct: 61  HGMWPSLYSGAQTHCFGSPFDVNQISILQPGISEIWPNVVTGKNQWLWGHEWDKHGTCTE 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISC 180
            +L DQ  YF + M+     N   IL  S I P  +  Y+  +I  AI AKTGK PA+ C
Sbjct: 121 SILFDQEEYFSFTMDKYIENNLSFILGKSQITPNGQ-LYSRAQIYAAISAKTGKTPAVRC 180

Query: 181 NYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWYP 223
           NY++W+  EQLHEI+ C   + + F DCP NANKC+  + MWYP
Sbjct: 181 NYNYWSGVEQLHEITLCYNWNATAFEDCPLNANKCDN-TFMWYP 222

[Close]

BLAST of HG10019715 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 264.6 bits (675), Expect = 7.7e-67
Identity = 129/222 (58.11%), Postives = 154/222 (69.37%), Query Frame = 0


Query: 1   MVRREII-LALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSI 60
           M +REII L LS+L  +A CEG+NE+DYL+VV QWQPATC D  K  P C QAP NRFSI
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNK--PACFQAPGNRFSI 60

Query: 61  HGMWPSLYNG-QTQCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           HG+WPSLY+G  TQCSGS FD NQ+  L+P +SE+W N+ TGDN WLW+HEWDKHGTC E
Sbjct: 61  HGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSE 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISC 180
               DQ  YF + M+   + N + IL N+ I P     YT D++  AIRAKTGK PA+ C
Sbjct: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGR-TYTRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMW 221
           NY+  T   QLHEIS C  +D S F DCP NANKC G S +W
Sbjct: 181 NYNSRTGASQLHEISLCFKKDASVFEDCPLNANKC-GASFVW 218

[Close]

BLAST of HG10019715 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 193.4 bits (490), Expect = 2.2e-45
Identity = 95/218 (43.58%), Postives = 133/218 (61.01%), Query Frame = 0


Query: 1   MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIH 60
           M +REI+L LS+   I+  EGVNE+DY +VV QWQPATC  AT     C Q P++R+S+H
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVH 60

Query: 61  GMWPSLYNGQ-TQCSGS-SFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           G+WPSL +G    CSG+  +D   +  ++ RL + W N+ITG N   W +EW KHGTC  
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTC-S 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISC 180
              L+Q  YF + +     ++ + IL   GI+P  +  YT   ++NAI + TGK PA+ C
Sbjct: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQ-QYTKTVVQNAIYSATGKWPALRC 180

Query: 181 NYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGY 217
           N ++ T   QLHEIS C +++G   +DCP N   CN Y
Sbjct: 181 NNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEY 215

[Close]

BLAST of HG10019715 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 61/184 (33.15%), Postives = 92/184 (50.00%), Query Frame = 0


Query: 25  YDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIHGMWP-SLYNGQTQC-SGSSFDINQ 84
           +D    V QW PA C  + +    C  +    F+IHG+WP       T C  GS FDI +
Sbjct: 1   FDSFWFVQQWPPAVC--SFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITK 60

Query: 85  VKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYYFNPEE 144
           +  L+ +L+ +W N++  +NQ  W HEW KHGTC E    +Q+ YF+ A++    ++   
Sbjct: 61  ISHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSES-TFNQAAYFKLAVDMRNNYDIIG 120

Query: 145 ILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTTRDGST 204
            L+     P      +   IK  ++AK GK P + C  D  T    L ++  C  +DGST
Sbjct: 121 ALRPHAAGPNGR-TKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGST 180

Query: 205 FVDC 207
            +DC
Sbjct: 181 LIDC 180

[Close]

BLAST of HG10019715 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 113.6 bits (283), Expect = 2.9e-24
Identity = 64/205 (31.22%), Postives = 99/205 (48.29%), Query Frame = 0


Query: 12  ILAFIARCEGV----NEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIHGMWPSLY 71
           IL  I +C  V     ++D+   V QW  + C             P   F IHG+WP+  
Sbjct: 12  ILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWPNNN 71

Query: 72  NG--QTQCS-GSSFDINQVKVLEPRLSEMWGNII--TGDNQWLWKHEWDKHGTCIEDMLL 131
           +G   + C   S +D +Q+  L   + + W  +   +G     W HEW+KHGTC E +L 
Sbjct: 72  DGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAESVLT 131

Query: 132 DQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDH 191
           +Q  YF+ A++     +   IL+ + I P  E  Y L  I+NAI++  G  P I CN D 
Sbjct: 132 NQHAYFKKALDLKNQIDLLSILQGADIHPDGES-YDLVNIRNAIKSAIGYTPWIQCNVDQ 191

Query: 192 WTSYEQLHEISYCTTRDGSTFVDCP 208
            +   QL+++  C    GS+ ++CP
Sbjct: 192 -SGNSQLYQVYICVDGSGSSLIECP 214

[Close]

BLAST of HG10019715 vs. ExPASy Swiss-Prot
Match: P80196 (Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2)

HSP 1 Score: 112.1 bits (279), Expect = 8.4e-24
Identity = 72/230 (31.30%), Postives = 111/230 (48.26%), Query Frame = 0


Query: 3   RREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKP--YPYCNQAPQNRFSIH 62
           ++ +I  + +   +  C    ++D+   V QW PA+  D  +   YP   + P   FSIH
Sbjct: 6   KKLLIKIIVVQCLLVLCVTSQDFDFFYFVQQW-PASYCDTRRSCCYPTTGK-PDEDFSIH 65

Query: 63  GMWPSLYNG---QTQCSGSSFDINQVKVLEPRLSEMWGNII--TGDNQWLWKHEWDKHGT 122
           G+WP+  +G   Q     SS D ++   L   + + W ++   + D    W HEW KHGT
Sbjct: 66  GLWPNYKDGKWPQNCDRESSLDESEFSDLISTMEKNWPSLACPSSDGLKFWSHEWLKHGT 125

Query: 123 CIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPA 182
           C     L+Q  YF+ A++     N  + L N+GI P    YY ++ IK AI    G  P 
Sbjct: 126 C---SALNQHAYFQTALDFKTKSNLLQNLNNAGIKPRNGDYYGVESIKKAIEKGVGHTPF 185

Query: 183 ISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP--SNANKCNGYSAMWYPS 224
           I CN D   ++ QL+++  C     S F+DCP   +  KC   S + +PS
Sbjct: 186 IECNVDSQGNH-QLYQVYLCVDSSASKFIDCPIFPHGGKCG--SKIEFPS 227

[Close]

BLAST of HG10019715 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 2.4e-23
Identity = 62/190 (32.63%), Postives = 98/190 (51.58%), Query Frame = 0


Query: 24  EYDYLRVVFQWQPATCGDATKPYPYCNQA-PQNRFSIHGMWPSLYNG--QTQCSGSS-FD 83
           ++D+   V QW  + C D  K   Y N   P   F IHG+WP+  +G   + C  S  FD
Sbjct: 29  DFDFFYFVQQWPGSYC-DTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPFD 88

Query: 84  INQVKVLEPRLSEMWGNII--TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYY 143
            + +  L   + + W  +   +G  +  W+HEW+KHGTC E  ++DQ  YF+ A+N    
Sbjct: 89  SSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES-VIDQHEYFQTALNLKQK 148

Query: 144 FNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTT 203
            N    L  +GI P  +  Y+L+ I+++I+   G  P + CN D  +   QL+++  C  
Sbjct: 149 TNLLGALTKAGINPDGKS-YSLESIRDSIKESIGFTPWVECNRD-GSGNSQLYQVYLCVD 208

Query: 204 RDGSTFVDCP 208
           R GS  ++CP
Sbjct: 209 RSGSGLIECP 214

[Close]

BLAST of HG10019715 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.1e-22
Identity = 65/190 (34.21%), Postives = 89/190 (46.84%), Query Frame = 0


Query: 24  EYDYLRVVFQWQPATCGDA-TKPYPYCNQAPQNRFSIHGMWPSLYNG---QTQCSGSSFD 83
           ++D+   V QW  A C    +  YP   + P   F IHG+WP+   G   Q     S FD
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGK-PAADFGIHGLWPNYKTGGWPQNCNPDSRFD 80

Query: 84  INQVKVLEPRLSEMWGNII--TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYY 143
             +V  L   L   W  +   + D    W HEW+KHGTC E   LDQ  YFE  +     
Sbjct: 81  DLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE-LDQHDYFEAGLKLKQK 140

Query: 144 FNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTT 203
            N    L N+GI P  + +Y + +I+N I+   G  P I CN D  +   QL++I  C  
Sbjct: 141 ANLLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVD 200

Query: 204 RDGSTFVDCP 208
              S F++CP
Sbjct: 201 TSASKFINCP 206

[Close]

BLAST of HG10019715 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.2e-73
Identity = 127/222 (57.21%), Postives = 161/222 (72.52%), Query Frame = 0


Query: 1   MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIH 60
           M +REI+L LS+L  IA CEG+NE+DYL+VV QWQPATC  + KPYP C Q P NR+SIH
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GMWPSLYNG-QTQCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIED 120
           G+WPSLY+G  TQCSGS F+ N + +L+P +SE+W NII G+N WLW HEWDKHGTC E+
Sbjct: 61  GVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEE 120

Query: 121 MLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCN 180
           +L DQ  YF +AM+T   +N   +L NS I P  +  Y+ D++  AIRAKTGK PA+ CN
Sbjct: 121 ILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQ-IYSRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 YDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWY 222
           Y+ WT  +Q+HE+S C   D S  VDCP NANKC+ Y  +WY
Sbjct: 181 YNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNY-FVWY 220

[Close]

BLAST of HG10019715 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 3.7e-67
Identity = 129/222 (58.11%), Postives = 154/222 (69.37%), Query Frame = 0


Query: 1   MVRREII-LALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSI 60
           M +REII L LS+L  +A CEG+NE+DYL+VV QWQPATC D  K  P C QAP NRFSI
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNK--PACFQAPGNRFSI 60

Query: 61  HGMWPSLYNG-QTQCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           HG+WPSLY+G  TQCSGS FD NQ+  L+P +SE+W N+ TGDN WLW+HEWDKHGTC E
Sbjct: 61  HGVWPSLYDGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSE 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISC 180
               DQ  YF + M+   + N + IL N+ I P     YT D++  AIRAKTGK PA+ C
Sbjct: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGR-TYTRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMW 221
           NY+  T   QLHEIS C  +D S F DCP NANKC G S +W
Sbjct: 181 NYNSRTGASQLHEISLCFKKDASVFEDCPLNANKC-GASFVW 218

[Close]

BLAST of HG10019715 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 1.1e-45
Identity = 95/218 (43.58%), Postives = 133/218 (61.01%), Query Frame = 0


Query: 1   MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIH 60
           M +REI+L LS+   I+  EGVNE+DY +VV QWQPATC  AT     C Q P++R+S+H
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLK-TVCVQPPEDRYSVH 60

Query: 61  GMWPSLYNGQ-TQCSGS-SFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           G+WPSL +G    CSG+  +D   +  ++ RL + W N+ITG N   W +EW KHGTC  
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTC-S 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISC 180
              L+Q  YF + +     ++ + IL   GI+P  +  YT   ++NAI + TGK PA+ C
Sbjct: 121 SAYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQ-QYTKTVVQNAIYSATGKWPALRC 180

Query: 181 NYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGY 217
           N ++ T   QLHEIS C +++G   +DCP N   CN Y
Sbjct: 181 NNNYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEY 215

[Close]

BLAST of HG10019715 vs. ExPASy TrEMBL
Match: A0A6J1DYI5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 3.5e-41
Identity = 97/218 (44.50%), Postives = 130/218 (59.63%), Query Frame = 0


Query: 1   MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIH 60
           M +REII  L +L FIA  E  + +DY +VVFQWQPATC D T P   C Q P NRFSIH
Sbjct: 1   MAKREIIFVLVLLLFIASSEQAS-FDYYQVVFQWQPATCNDITLP-TRCVQNPDNRFSIH 60

Query: 61  GMWPSLYNGQT--QCSGSSFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           G+WPS  +G     C+G++FD   +  +   L++ W ++I   NQ  W  EW KHGTC E
Sbjct: 61  GVWPSKTSGSRVGPCNGTAFDPATIVSIRAGLNQNWPDVIRRKNQVFWSDEWRKHGTCSE 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSG---IWPTQEGYYTLDEIKNAIRAKTGKLPA 180
           D LL+Q  YF + +N +Y+   ++I++  G   I P  + Y   D ++ AI +  GK   
Sbjct: 121 D-LLNQLQYFTFGLN-IYHDEGKDIVEKLGKKKIIPNGKKYKKAD-VEKAISSVIGKRLG 180

Query: 181 ISCNYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKC 214
             CN D  ++Y QLHEIS+C  +D ST  DCPSNA  C
Sbjct: 181 FRCNIDQ-SNYHQLHEISFCYAKDASTLQDCPSNATNC 212

[Close]

BLAST of HG10019715 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 5.1e-40
Identity = 90/224 (40.18%), Postives = 124/224 (55.36%), Query Frame = 0


Query: 1   MVRREIILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIH 60
           M ++ I+L  S L  IA C    +Y+Y +V  QWQPATC DA    P C   PQ+RFSIH
Sbjct: 1   MEKKGIVLVFSTLVVIASC----DYNYYQVTLQWQPATCSDANLQTP-CVNTPQDRFSIH 60

Query: 61  GMWPSLYNGQ-TQCSGS-SFDINQVKVLEPRLSEMWGNIITGDNQWLWKHEWDKHGTCIE 120
           G+WP+L +G   QC G+ ++D +++  +  RL+  W ++I G N  LW HEW  HGTC  
Sbjct: 61  GVWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSY 120

Query: 121 DMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISC 180
              L Q  YF + ++T   ++    L    I+P+ E  Y+   I NAIR  T KLP + C
Sbjct: 121 PS-LSQLDYFTFGLDTYAKYDIYSALDKEDIYPS-EDVYSKSSINNAIRKITQKLPGLRC 180

Query: 181 NYDHWTSYEQLHEISYCTTRDGSTFVDCPSNANKCNGYSAMWYP 223
           N + ++   QLHEI  C  +     +DCPSN   C  Y   WYP
Sbjct: 181 NTNDYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNY-VYWYP 216

[Close]

BLAST of HG10019715 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 129.0 bits (323), Expect = 4.7e-30
Identity = 75/208 (36.06%), Postives = 107/208 (51.44%), Query Frame = 0


Query: 6   IILALSILAFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSIHGMWPS 65
           ++L L +   +       ++D+   V QW  A C  +       +  P   F IHG+WP+
Sbjct: 9   VLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWPN 68

Query: 66  LYNGQTQCSG----SSFDINQVKVLEPRLSEMWGNIITGDNQW--LWKHEWDKHGTCIED 125
            YNG +  S     S FD +Q+  L   L + W  +    N+    W+HEW+KHGTC E 
Sbjct: 69  -YNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSES 128

Query: 126 MLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCN 185
            ++DQ  YFE A+      N  +ILKNSGI P  +G+Y LD+I NAI+   G  P I CN
Sbjct: 129 -VMDQHEYFENALKLKQKANLLQILKNSGINP-DDGFYNLDKITNAIKDGIGFTPGIECN 188

Query: 186 YDHWTSYEQLHEISYCTTRDGSTFVDCP 208
            D   +  QLH+I  C    G+ F++CP
Sbjct: 189 KDPERN-AQLHQIYICVDTSGTEFIECP 212

[Close]

BLAST of HG10019715 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 110.5 bits (275), Expect = 1.7e-24
Identity = 62/190 (32.63%), Postives = 98/190 (51.58%), Query Frame = 0


Query: 24  EYDYLRVVFQWQPATCGDATKPYPYCNQA-PQNRFSIHGMWPSLYNG--QTQCSGSS-FD 83
           ++D+   V QW  + C D  K   Y N   P   F IHG+WP+  +G   + C  S  FD
Sbjct: 29  DFDFFYFVQQWPGSYC-DTQKKCCYPNSGKPAADFGIHGLWPNYKDGTYPSNCDASKPFD 88

Query: 84  INQVKVLEPRLSEMWGNII--TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYY 143
            + +  L   + + W  +   +G  +  W+HEW+KHGTC E  ++DQ  YF+ A+N    
Sbjct: 89  SSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSES-VIDQHEYFQTALNLKQK 148

Query: 144 FNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTT 203
            N    L  +GI P  +  Y+L+ I+++I+   G  P + CN D  +   QL+++  C  
Sbjct: 149 TNLLGALTKAGINPDGKS-YSLESIRDSIKESIGFTPWVECNRD-GSGNSQLYQVYLCVD 208

Query: 204 RDGSTFVDCP 208
           R GS  ++CP
Sbjct: 209 RSGSGLIECP 214

[Close]

BLAST of HG10019715 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 107.5 bits (267), Expect = 1.5e-23
Identity = 65/190 (34.21%), Postives = 89/190 (46.84%), Query Frame = 0


Query: 24  EYDYLRVVFQWQPATCGDA-TKPYPYCNQAPQNRFSIHGMWPSLYNG---QTQCSGSSFD 83
           ++D+   V QW  A C    +  YP   + P   F IHG+WP+   G   Q     S FD
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGK-PAADFGIHGLWPNYKTGGWPQNCNPDSRFD 80

Query: 84  INQVKVLEPRLSEMWGNII--TGDNQWLWKHEWDKHGTCIEDMLLDQSGYFEYAMNTLYY 143
             +V  L   L   W  +   + D    W HEW+KHGTC E   LDQ  YFE  +     
Sbjct: 81  DLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESE-LDQHDYFEAGLKLKQK 140

Query: 144 FNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTSYEQLHEISYCTT 203
            N    L N+GI P  + +Y + +I+N I+   G  P I CN D  +   QL++I  C  
Sbjct: 141 ANLLHALTNAGIKP-DDKFYEMKDIENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVD 200

Query: 204 RDGSTFVDCP 208
              S F++CP
Sbjct: 201 TSASKFINCP 206

[Close]

BLAST of HG10019715 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.5e-23
Identity = 68/215 (31.63%), Postives = 104/215 (48.37%), Query Frame = 0


Query: 2   VRREIILALSIL--AFIARCEGVNEYDYLRVVFQWQPATCGDATKPYPYCNQAPQNRFSI 61
           +R  II++L IL    ++  +   ++++   V  W  A C       P       + F I
Sbjct: 1   MRGIIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCCPPTKGNTASDFII 60

Query: 62  HGMWPSLYNG--QTQCSGSS-FDINQVKVLEPRLSEMWGN----IITGDNQWLWKHEWDK 121
           HG+WP   NG     C  ++ FDI+++  L  ++ + W          +   LW+HEW+K
Sbjct: 61  HGLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNK 120

Query: 122 HGTCIEDMLLDQSGYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGK 181
           HGTC++  + DQ  YF   +   +  +   IL   GI P  +G+Y+LDEIKNAI+   G 
Sbjct: 121 HGTCVQS-IFDQHSYFRTNLKFKHKVHLLNILIQKGIKP-NDGFYSLDEIKNAIKCAIGF 180

Query: 182 LPAISCNYDHWTSYEQLHEISYCTTRDGSTFVDCP 208
            P I CN D     +QL +I  C       FV+CP
Sbjct: 181 APGIECNED-VKGNKQLFQIYICLDNYAKEFVECP 212

[Close]

BLAST of HG10019715 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 79.3 bits (194), Expect = 4.3e-15
Identity = 53/194 (27.32%), Postives = 80/194 (41.24%), Query Frame = 0


Query: 24  EYDYLRVVFQWQPATCGDATKPYPYC---------NQAPQNRFSIHGMWPSLYNGQ--TQ 83
           E+DY  +  QW P T    T+   +C         + AP  +F+IHG+WP   +G   + 
Sbjct: 31  EFDYFALSLQW-PGTYCRGTR---HCCSKNACCRGSDAP-TQFTIHGLWPDYNDGSWPSC 90

Query: 84  CSGSSFDINQVKVLEPRLSEMWGNI-------ITGDNQWLWKHEWDKHGTCIEDMLLDQS 143
           C  S F   ++  L   L + W ++         G     W HEW+KHGTC   +  D+ 
Sbjct: 91  CYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEY 150

Query: 144 GYFEYAMNTLYYFNPEEILKNSGIWPTQEGYYTLDEIKNAIRAKTGKLPAISCNYDHWTS 200
            YF   +N     N  ++L  +G   +    Y L  I  AI+      P + C  D    
Sbjct: 151 NYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRD---- 210

The following BLAST results are available for this feature:

BLAST of HG10019715 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904516.1	1.5e-94	67.36	ribonuclease MC-like [Benincasa hispida]	[more]
AEM42996.1	2.5e-73	57.21	S-RNase [Siraitia grosvenorii]	[more]
XP_038903744.1	3.3e-70	57.14	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022149793.1	7.7e-67	58.11	ribonuclease MC-like [Momordica charantia]	[more]
XP_022158939.1	2.2e-45	43.58	ribonuclease MC-like [Momordica charantia]	[more]

BLAST of HG10019715 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P23540	1.7e-24	33.15	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P80022	2.9e-24	31.22	Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2	[more]
P80196	8.4e-24	31.30	Intracellular ribonuclease LX OS=Solanum lycopersicum OX=4081 GN=RNALX PE=1 SV=2	[more]
P42813	2.4e-23	32.63	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P42815	2.1e-22	34.21	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]

BLAST of HG10019715 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
K7NBR4	1.2e-73	57.21	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	3.7e-67	58.11	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]
A0A6J1E0X1	1.1e-45	43.58	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DYI5	3.5e-41	44.50	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1	[more]
A0A6J1E266	5.1e-40	40.18	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]

BLAST of HG10019715 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14220.1	4.7e-30	36.06	Ribonuclease T2 family protein	[more]
AT2G02990.1	1.7e-24	32.63	ribonuclease 1	[more]
AT1G26820.1	1.5e-23	34.21	ribonuclease 3	[more]
AT1G14210.1	1.5e-23	31.63	Ribonuclease T2 family protein	[more]
AT2G39780.1	4.3e-15	27.32	ribonuclease 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 18..224 e-value: 9.0E-51 score: 174.8
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 6..222
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 25..206 e-value: 9.7E-42 score: 143.2
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 16..214
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 16..214
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 105..116
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 25..214 e-value: 2.23306E-53 score: 168.28

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10019715.1	HG10019715.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016070	RNA metabolic process
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding