HG10019487 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10019487
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 5
LocationChr04: 22356032 .. 22357336 (+)
RNA-Seq ExpressionHG10019487
SyntenyHG10019487
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGGTGAGGCAGCGCTGGCGAGGAATGTTCTATTTGGGAAATACGAAATGGGTCGGCTATTGGGGAAAGGAACCTTTGCGAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCAATTAAAGTTATCCTCAAGGATAGGGTGAAGAAAGAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGGTTGGTTCGTCATCCCCATATTGTGGAATTGAAAGAAGTAATGGCAACGAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAATTGTCTCAACAAGGAAAATTGACTGAAGATGGAGCAAGAAAGTACTTTCAACAACTTATCTCCGCCGTGGACTTTTGCCACAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAAAATCTCCTTCTCGACGAGAATGGGAATTTGAAGGTATCTGATTTTGGCCTCTCCGCCCTGCCTGAGCAGCTCCGGATCGATGGTTTGCTTCACACACGGTGTGGAACTCCGGCGTATGTGGCGCCTGAGGTACTCAGAAAGAAGGGTTACGACGGCGCTAAGGCGGATATTTGGTCTTGTGGGGTGATTTTGTTTGTGTTGCTTGCTGGGACTCTGCCATTTCGAGCTGATAATGTGATGAAGTTGTATAGGAAGATTTTCAAGGCAGAGTTTGAGTTTCCTCCATGGTTTTCTTCAGAAGCCATGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGGTTGAGATAATGCAGAGTCCTTGGTTTCAAAAGGGCTTCACAAAGCCAGTTTCATTCATTGAGAAAGATGAATTCGACAATGAATTCTCTGAATTGGCAAATCCTAAGTTGCCATCTTCTCCTCCTTTTTACAATGCCTTTGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACCTGTCTAGTCTGTTTGAAAGCAAGAAGAAACGAGCCTCAATTTTCACTTCAAAATGCTCTGCTTCAGCCATTATTGCCAAGCTTCAGTCTCTGGCCGTGAAACTCAACTTCAAGGCTACTAACCTGAAGGAGTTCAAGGTTAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACGGTGACCGCGGAGGTTTACAAGGCGGCACCGGAGCTGGCAGTGGTTGAGTTCTCGAAGTCGGCCGGTGACACTTTGGAATACAATAAGTTCTGTGAGAAGGATGTGAGGCCTGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAGAATGAGTGCCTTTAA

mRNA sequence

ATGGCAGGTGAGGCAGCGCTGGCGAGGAATGTTCTATTTGGGAAATACGAAATGGGTCGGCTATTGGGGAAAGGAACCTTTGCGAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCAATTAAAGTTATCCTCAAGGATAGGGTGAAGAAAGAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGGTTGGTTCGTCATCCCCATATTGTGGAATTGAAAGAAGTAATGGCAACGAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAATTGTCTCAACAAGGAAAATTGACTGAAGATGGAGCAAGAAAGTACTTTCAACAACTTATCTCCGCCGTGGACTTTTGCCACAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAAAATCTCCTTCTCGACGAGAATGGGAATTTGAAGGTATCTGATTTTGGCCTCTCCGCCCTGCCTGAGCAGCTCCGGATCGATGGTTTGCTTCACACACGGTGTGGAACTCCGGCGTATGTGGCGCCTGAGGTACTCAGAAAGAAGGGTTACGACGGCGCTAAGGCGGATATTTGGTCTTGTGGGGTGATTTTGTTTGTGTTGCTTGCTGGGACTCTGCCATTTCGAGCTGATAATGTGATGAAGTTGTATAGGAAGATTTTCAAGGCAGAGTTTGAGTTTCCTCCATGGTTTTCTTCAGAAGCCATGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGGTTGAGATAATGCAGAGTCCTTGGTTTCAAAAGGGCTTCACAAAGCCAGTTTCATTCATTGAGAAAGATGAATTCGACAATGAATTCTCTGAATTGGCAAATCCTAAGTTGCCATCTTCTCCTCCTTTTTACAATGCCTTTGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACCTGTCTAGTCTGTTTGAAAGCAAGAAGAAACGAGCCTCAATTTTCACTTCAAAATGCTCTGCTTCAGCCATTATTGCCAAGCTTCAGTCTCTGGCCGTGAAACTCAACTTCAAGGCTACTAACCTGAAGGAGTTCAAGGTTAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACGGTGACCGCGGAGGTTTACAAGGCGGCACCGGAGCTGGCAGTGGTTGAGTTCTCGAAGTCGGCCGGTGACACTTTGGAATACAATAAGTTCTGTGAGAAGGATGTGAGGCCTGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAGAATGAGTGCCTTTAA

Coding sequence (CDS)

ATGGCAGGTGAGGCAGCGCTGGCGAGGAATGTTCTATTTGGGAAATACGAAATGGGTCGGCTATTGGGGAAAGGAACCTTTGCGAAGGTCTATTATGGAAGAAACTTGATGACCCACGAGAGTGTTGCAATTAAAGTTATCCTCAAGGATAGGGTGAAGAAAGAGGGGCTTCTTGAGCAAATCCGGCGAGAAATTTCTGTTATGGGGTTGGTTCGTCATCCCCATATTGTGGAATTGAAAGAAGTAATGGCAACGAAATCGAAGGTGTATTTTGTGATGGAGTATGTTAATGGAGGCGAACTGTTTGTGAAATTGTCTCAACAAGGAAAATTGACTGAAGATGGAGCAAGAAAGTACTTTCAACAACTTATCTCCGCCGTGGACTTTTGCCACAGCCGTGGTGTCTCTCACCGTGATTTGAAGCCTGAAAATCTCCTTCTCGACGAGAATGGGAATTTGAAGGTATCTGATTTTGGCCTCTCCGCCCTGCCTGAGCAGCTCCGGATCGATGGTTTGCTTCACACACGGTGTGGAACTCCGGCGTATGTGGCGCCTGAGGTACTCAGAAAGAAGGGTTACGACGGCGCTAAGGCGGATATTTGGTCTTGTGGGGTGATTTTGTTTGTGTTGCTTGCTGGGACTCTGCCATTTCGAGCTGATAATGTGATGAAGTTGTATAGGAAGATTTTCAAGGCAGAGTTTGAGTTTCCTCCATGGTTTTCTTCAGAAGCCATGGAAATGGTGTCTAAGCTTCTGGTTGTTGATCCTCAAAAGAGAGCTTCAATGGTTGAGATAATGCAGAGTCCTTGGTTTCAAAAGGGCTTCACAAAGCCAGTTTCATTCATTGAGAAAGATGAATTCGACAATGAATTCTCTGAATTGGCAAATCCTAAGTTGCCATCTTCTCCTCCTTTTTACAATGCCTTTGAGTTCATTTCCTCAATGTCCTCTGGTTTTGACCTGTCTAGTCTGTTTGAAAGCAAGAAGAAACGAGCCTCAATTTTCACTTCAAAATGCTCTGCTTCAGCCATTATTGCCAAGCTTCAGTCTCTGGCCGTGAAACTCAACTTCAAGGCTACTAACCTGAAGGAGTTCAAGGTTAGACTGCAGGCCAAGGAGGAGGGGAGGAAAGGGAAGTTGACGGTGACCGCGGAGGTTTACAAGGCGGCACCGGAGCTGGCAGTGGTTGAGTTCTCGAAGTCGGCCGGTGACACTTTGGAATACAATAAGTTCTGTGAGAAGGATGTGAGGCCTGCATTGAAAGACATAGTTTGGAGCTGGCAAGGAGAGAATGAGTGCCTTTAA

Protein sequence

MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPKLPSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIVWSWQGENECL
Homology
BLAST of HG10019487 vs. NCBI nr
Match: XP_038905015.1 (CBL-interacting serine/threonine-protein kinase 25-like [Benincasa hispida])

HSP 1 Score: 826.2 bits (2133), Expect = 1.3e-235
Identity = 418/434 (96.31%), Postives = 421/434 (97.00%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAGEA    NVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAPPRMNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPKLP 300
           SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSF EKDEF NEFSELANPK  
Sbjct: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFAEKDEFSNEFSELANPKFL 300

Query: 301 SSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKAT 360
           SSPPFYNAFEFISSMSSGFDLSSLFESKK+R SIFTSKCS SAIIAKLQSLAVKLNFKAT
Sbjct: 301 SSPPFYNAFEFISSMSSGFDLSSLFESKKRRGSIFTSKCSPSAIIAKLQSLAVKLNFKAT 360

Query: 361 NLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPAL 420
           NL+EFKVRLQAKEEGRKGKL VTAEVY+ APELAVVEFSKS GDTLEYNKFCEKDVRPAL
Sbjct: 361 NLREFKVRLQAKEEGRKGKLAVTAEVYEVAPELAVVEFSKSTGDTLEYNKFCEKDVRPAL 420

Query: 421 KDIVWSWQGENECL 435
           KDIVWSWQGENECL
Sbjct: 421 KDIVWSWQGENECL 434

BLAST of HG10019487 vs. NCBI nr
Match: XP_008465153.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo] >KAA0037705.1 CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo var. makuwa] >TYK27019.1 CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo var. makuwa])

HSP 1 Score: 813.1 bits (2099), Expect = 1.1e-231
Identity = 412/433 (95.15%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAGEAA  RNVLFGKYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGAR YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD+NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GYDGAKADIWSCGVILFVLLAGTLPFRADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDPQKRASMVEIM SPWFQ+GFTKPVSF+EKDEF+NEFSELANPK L
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDLSSLFESKKK+ SIFTSKC ASAIIAKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV++ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 433
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of HG10019487 vs. NCBI nr
Match: XP_004137659.1 (CBL-interacting serine/threonine-protein kinase 25 [Cucumis sativus])

HSP 1 Score: 805.8 bits (2080), Expect = 1.8e-229
Identity = 410/433 (94.69%), Postives = 418/433 (96.54%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG AA  RNVLF KYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGGAAQVRNVLFRKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQ GKLTEDGAR YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQLGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GYDGAKADIWSCGVILFVLLAGTLPFRADN MKLYRKIFKAE+EFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEYEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDPQKRASMVEIM SPWFQKGFTKPVSFIEKDEF+NEFSELANPK L
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQKGFTKPVSFIEKDEFNNEFSELANPKLL 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDLSSLFESKKK+ SIFTSKC ASAI+AKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAILAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TNLKEFKVRLQAKEEGRKGKLTVTAEVY+ APELA+VEFSKSAGDTLEYNK CEKDVRPA
Sbjct: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYEVAPELAIVEFSKSAGDTLEYNKLCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 433
           LKDIVWSWQGE+E
Sbjct: 421 LKDIVWSWQGESE 433

BLAST of HG10019487 vs. NCBI nr
Match: KAG6581626.1 (CBL-interacting serine/threonine-protein kinase 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 791.6 bits (2043), Expect = 3.5e-225
Identity = 398/435 (91.49%), Postives = 419/435 (96.32%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG+AA ARNVLFGKYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDG RKYF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GY+GAK+D+WSCGVILFVLLAG+LPF+ADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDP+KRASM EIMQS WFQKGFTKP+SF +KDE ++E S+L NPK L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDL+SLFESKKK+ASIFTSKCSASAIIAKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVY+ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 435
           LKDIVWSWQGENECL
Sbjct: 421 LKDIVWSWQGENECL 435

BLAST of HG10019487 vs. NCBI nr
Match: XP_022982781.1 (CBL-interacting serine/threonine-protein kinase 25-like [Cucurbita maxima])

HSP 1 Score: 789.6 bits (2038), Expect = 1.3e-224
Identity = 399/435 (91.72%), Postives = 418/435 (96.09%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG+AA ARNVLFGKYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVME VNGGELFVKLSQQGKLTEDG RKYF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMECVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GY+GAK+D+WSCGVILFVLLAG+LPF+ADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDP+KRASM EIMQS WFQKGFTKP+SF +KDEF +E SELANPK L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDEFKSEISELANPKML 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDL+SLFESKKK+ASIFTSKCSASAIIAKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN +EFKVRLQA EEGRKGKL VT EVY+ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNQREFKVRLQAMEEGRKGKLAVTMEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 435
           LKDIVWSWQGENECL
Sbjct: 421 LKDIVWSWQGENECL 435

BLAST of HG10019487 vs. ExPASy Swiss-Prot
Match: Q8W1D5 (CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CIPK25 PE=2 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 2.6e-167
Identity = 294/430 (68.37%), Postives = 353/430 (82.09%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           VLF KYEMGRLLGKGTF KVYYG+ + T ESVAIK+I KD+VK+EG++EQI+REIS+M L
Sbjct: 38  VLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREISIMRL 97

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAVDFC 130
           VRHP+IVELKEVMATK+K++F+MEYV GGELF K+  +GKL ED ARKYFQQLISAVDFC
Sbjct: 98  VRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKI-VKGKLKEDSARKYFQQLISAVDFC 157

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGVSHRDLKPENLL+DENG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPEVLRK
Sbjct: 158 HSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLRK 217

Query: 191 KGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEMVSK 250
           KGYDGAK DIWSCG+IL+VLLAG LPF+ +N+MK+YRKIFK+EFE+PPWFS E+  ++SK
Sbjct: 218 KGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKRLISK 277

Query: 251 LLVVDPQKRASMVEIMQSPWFQKGFTKPVSF-IEKDEFDNEFSE--------LANPKLPS 310
           LLVVDP KR S+  IM++PWF+K    P+ F I++ E  N   E              P 
Sbjct: 278 LLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTTTTPV 337

Query: 311 SPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKATN 370
           SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+ SAS I+ KL+ +  ++N K   
Sbjct: 338 SPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNMKVKR 397

Query: 371 LKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALK 430
            K+FKV+L  K EGRKG++ VTAEV++ APE+AVVE  KSAGDTLEYN+  E+ VRPAL+
Sbjct: 398 TKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVRPALE 457

Query: 431 DIVWSWQGEN 432
           +IVWSW G+N
Sbjct: 458 EIVWSWHGDN 466

BLAST of HG10019487 vs. ExPASy Swiss-Prot
Match: Q9LEU7 (CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CIPK5 PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.4e-167
Identity = 289/434 (66.59%), Postives = 362/434 (83.41%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIRREISV 68
           R VLFGKYEMGRLLGKGTFAKVYYG+ ++  E VAIKVI KD+V K+ G++EQI+REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S +GKL ED AR+YFQQLISAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKIS-KGKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLDENG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEM 248
           L+KKGYDGAKADIWSCGV+L+VLLAG LPF+ +N+M +YRKIF+A+FEFPPWFS EA  +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSF----------IEKDEFDNEFSELANP 308
           +SKLLVVDP +R S+  IM++PW +K FT P++F           + +E + E  +  N 
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 KLPSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNF 368
             P SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+ SA+ ++ K++++  ++N 
Sbjct: 305 TEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNM 364

Query: 369 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVR 428
           K    K+FKV+++ K EGRKG+L++TAEV++ APE++VVEF KSAGDTLEY++  E++VR
Sbjct: 365 KVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVR 424

Query: 429 PALKDIVWSWQGEN 432
           PAL DIVWSW G+N
Sbjct: 425 PALNDIVWSWHGDN 437

BLAST of HG10019487 vs. ExPASy Swiss-Prot
Match: Q9SEZ7 (CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=3702 GN=CIPK16 PE=1 SV=1)

HSP 1 Score: 520.0 bits (1338), Expect = 2.6e-146
Identity = 269/446 (60.31%), Postives = 337/446 (75.56%), Query Frame = 0

Query: 4   EAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIR 63
           E+  +  VLF KY +GRLLG G FAKVY+G  + T + VAIKVI KD V K+ G++EQI 
Sbjct: 3   ESNRSSTVLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIE 62

Query: 64  REISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQ 123
           REI+VM L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED ARKYFQQ
Sbjct: 63  REIAVMRLLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQ 122

Query: 124 LISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSAL--PEQL-----RIDGLLHT 183
           LISAVDFCHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHT
Sbjct: 123 LISAVDFCHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHT 182

Query: 184 RCGTPAYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFE 243
           RCGTPAYVAPEVLR KGYDGA ADIWSCG++L+ LLAG LPF  +NVM LY KIFKAE E
Sbjct: 183 RCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECE 242

Query: 244 FPPWFSSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELA 303
           FPPWFS E+ E++S+LLV DP++R SM EI   PWF+K FT  V+F   +   +      
Sbjct: 243 FPPWFSLESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPT 302

Query: 304 NPKLP---------SSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIA 363
             K           +SP  +NAF+FI+SMSSGFDLS+LFE K+K   +FTSK  A ++  
Sbjct: 303 KKKKKDLNEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKE 362

Query: 364 KLQSLAVKLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTL 423
           +L++ A +++ +  ++K+ K++LQ + EGRKG+L+VTAEV++ APE++VVEF K++GDTL
Sbjct: 363 RLETAAREMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTL 422

Query: 424 EYNKFCEKDVRPALKDIVWSWQGENE 433
           EY  FCE DVRPALKDIVWSWQG+++
Sbjct: 423 EYYLFCEDDVRPALKDIVWSWQGDDD 448

BLAST of HG10019487 vs. ExPASy Swiss-Prot
Match: Q6ERS4 (CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK16 PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 9.1e-144
Identity = 251/426 (58.92%), Postives = 337/426 (79.11%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKK-EGLLEQIRREISV 68
           R ++ G+YE+GRLLG+GTFAKVYYGR+L + ESVAIKVI K R+++ EG++EQ+RREIS+
Sbjct: 15  RKLVLGRYELGRLLGQGTFAKVYYGRDLRSGESVAIKVIDKARLRRTEGMVEQLRREISI 74

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAV 128
           M +VRHP++V ++EV+A++++V+ VMEY  GGELF K++ +G+LTE+ AR+YFQQL++AV
Sbjct: 75  MRMVRHPNVVGIREVLASRARVFVVMEYARGGELFAKVA-RGRLTEEHARRYFQQLVAAV 134

Query: 129 DFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
            FCH RGV+HRDLKPENLLLDE G LKV+DFGL+ALPEQLR DGLLHT+CGTPAYVAPEV
Sbjct: 135 GFCHGRGVAHRDLKPENLLLDEEGRLKVTDFGLAALPEQLRQDGLLHTQCGTPAYVAPEV 194

Query: 189 LRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEM 248
           LRK+GYDGA+AD+WSCGV+L+VLL G LPF+ +N  K+Y+KIFKAE++ PPW S +A  +
Sbjct: 195 LRKRGYDGARADLWSCGVVLYVLLCGFLPFQHENYAKMYQKIFKAEYQVPPWVSGDARRL 254

Query: 249 VSKLLVVDPQKRASMVEIMQSPWFQKGFTKPV--SFIEKDEFDNEFSEL-ANPKLPSSPP 308
           + +LLVVDP KR S+ EIM++PWF+KGF  PV  S +   +++ +   L        SP 
Sbjct: 255 IVRLLVVDPAKRISIPEIMRTPWFKKGFVPPVPTSPVSPKKWEEDDVLLDGGDSGAMSPR 314

Query: 309 FYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKATNLKE 368
             NAF+ ISSMSSGFDLS +FES++K A++FTS+  A+ +I KL+++   L + AT  K 
Sbjct: 315 TCNAFQLISSMSSGFDLSGMFESEQKAATVFTSRAPAATVIQKLEAVGRSLGYSATRGKG 374

Query: 369 FKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIV 428
           +K+RL+A  +G  G+L VT E  + A ++AVVEF+  AGD LE+NKFC  DVRP L DIV
Sbjct: 375 WKLRLEATADGANGRLAVTVEALEVAADVAVVEFAHDAGDELEFNKFCAVDVRPGLADIV 434

Query: 429 WSWQGE 431
           W+WQG+
Sbjct: 435 WAWQGD 439

BLAST of HG10019487 vs. ExPASy Swiss-Prot
Match: Q9FJ54 (CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=3702 GN=CIPK20 PE=1 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.7e-135
Identity = 244/424 (57.55%), Postives = 314/424 (74.06%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           VL  KYE+GRLLG+GTFAKVY+ RN+ T ESVAIKVI K +V K GL++QI+REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S +GKL E+ ARKYFQQLI A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVS-KGKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLDENG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 KGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEMVSK 250
           KGYDGAKAD+WSCGV+L+VLLAG LPF   N++++YRKI K EF+ P WF  E  +++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPQKRASMVEIMQSPWFQKGFTK---PVSFIEKDEFDNEFSELANPKLPSSPPFYN 310
           +L  +P  R  + +IM++ WFQKGF K   P S  E  + D+  S++ +      P  YN
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV-HAAFSVKPMSYN 306

Query: 311 AFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKATNLKEFKV 370
           AF+ ISS+S GFDLS LFE +++  S FT+K  A  I++K + +A           +  V
Sbjct: 307 AFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVGV 366

Query: 371 RLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIVWSW 430
           +++ K EGRKG L +  E+++      +VEF KS GDT+EY +FC++++RP+LKDIVW W
Sbjct: 367 KMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWKW 426

Query: 431 QGEN 432
           QG N
Sbjct: 427 QGNN 427

BLAST of HG10019487 vs. ExPASy TrEMBL
Match: A0A5A7T8B4 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00290 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.4e-232
Identity = 412/433 (95.15%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAGEAA  RNVLFGKYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGAR YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD+NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GYDGAKADIWSCGVILFVLLAGTLPFRADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDPQKRASMVEIM SPWFQ+GFTKPVSF+EKDEF+NEFSELANPK L
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDLSSLFESKKK+ SIFTSKC ASAIIAKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV++ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 433
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of HG10019487 vs. ExPASy TrEMBL
Match: A0A1S3CN52 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103502821 PE=3 SV=1)

HSP 1 Score: 813.1 bits (2099), Expect = 5.4e-232
Identity = 412/433 (95.15%), Postives = 422/433 (97.46%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAGEAA  RNVLFGKYEMGRLLGKGTFAKVYYGRN+MTHESVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGEAAQVRNVLFGKYEMGRLLGKGTFAKVYYGRNVMTHESVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGAR YF
Sbjct: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARNYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQLISAVDFCHSRGVSHRDLKPENLLLD+NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDQNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GYDGAKADIWSCGVILFVLLAGTLPFRADN MKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKADIWSCGVILFVLLAGTLPFRADNAMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDPQKRASMVEIM SPWFQ+GFTKPVSF+EKDEF+NEFSELANPK L
Sbjct: 241 SSEAKEMVSKLLVVDPQKRASMVEIMHSPWFQEGFTKPVSFLEKDEFNNEFSELANPKLL 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDLSSLFESKKK+ SIFTSKC ASAIIAKLQSLAVKLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLSSLFESKKKQGSIFTSKCPASAIIAKLQSLAVKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TNL+EFKVRLQAKEEGRKGKLTVTAEV++ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNLREFKVRLQAKEEGRKGKLTVTAEVHEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENE 433
           LKDIVWSWQGENE
Sbjct: 421 LKDIVWSWQGENE 433

BLAST of HG10019487 vs. ExPASy TrEMBL
Match: A0A6J1J0B3 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111481539 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.4e-225
Identity = 399/435 (91.72%), Postives = 418/435 (96.09%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG+AA ARNVLFGKYEMGRLLGKGTFAKVY+GRNLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGRNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVME VNGGELFVKLSQQGKLTEDG RKYF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMECVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GY+GAK+D+WSCGVILFVLLAG+LPF+ADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDP+KRASM EIMQS WFQKGFTKP+SF +KDEF +E SELANPK L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSSWFQKGFTKPLSFNDKDEFKSEISELANPKML 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDL+SLFESKKK+ASIFTSKCSASAIIAKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN +EFKVRLQA EEGRKGKL VT EVY+ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNQREFKVRLQAMEEGRKGKLAVTMEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 435
           LKDIVWSWQGENECL
Sbjct: 421 LKDIVWSWQGENECL 435

BLAST of HG10019487 vs. ExPASy TrEMBL
Match: A0A6J1F2J4 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111441609 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 9.3e-224
Identity = 396/435 (91.03%), Postives = 417/435 (95.86%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAG+AA ARNVLFGKYEMGRLLGKGTFAKVY+G NLMTH+SVAIKVILKDRVKKEGLLEQ
Sbjct: 1   MAGKAAPARNVLFGKYEMGRLLGKGTFAKVYHGTNLMTHDSVAIKVILKDRVKKEGLLEQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           +RREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDG RKYF
Sbjct: 61  VRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGGRKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           QQL+SAVDFCHSRGVSHRDLKPENLLLD NGNLKVSDFGLSALPEQLRIDGLLHTRCGTP
Sbjct: 121 QQLLSAVDFCHSRGVSHRDLKPENLLLDVNGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GY+GAK+D+WSCGVILFVLLAG+LPF+ADNVMKLYRKIFKAEFEFPPWF
Sbjct: 181 AYVAPEVLRKRGYEGAKSDLWSCGVILFVLLAGSLPFKADNVMKLYRKIFKAEFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPK-L 300
           SSEA EMVSKLLVVDP+KRASM EIMQS WFQKGFTKP+SF +KDE ++E S+L NPK L
Sbjct: 241 SSEAKEMVSKLLVVDPEKRASMDEIMQSCWFQKGFTKPLSFNDKDELNSEISDLTNPKLL 300

Query: 301 PSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKA 360
            SSPPFYNAFEFISSMSSGFDL+SLFESKKK+ASIFTSKCSASAIIAKLQSLA KLNFKA
Sbjct: 301 SSSPPFYNAFEFISSMSSGFDLTSLFESKKKQASIFTSKCSASAIIAKLQSLAQKLNFKA 360

Query: 361 TNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420
           TN +EFKVRLQAKEEGRKGKL VT EVY+ APELAVVEFSKSAGDTLEYNKFCEKDVRPA
Sbjct: 361 TNQREFKVRLQAKEEGRKGKLAVTVEVYEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPA 420

Query: 421 LKDIVWSWQGENECL 435
           LKDIVWSWQGENECL
Sbjct: 421 LKDIVWSWQGENECL 435

BLAST of HG10019487 vs. ExPASy TrEMBL
Match: A0A6J1DJA1 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111020996 PE=3 SV=1)

HSP 1 Score: 777.3 bits (2006), Expect = 3.3e-221
Identity = 388/434 (89.40%), Postives = 415/434 (95.62%), Query Frame = 0

Query: 1   MAGEAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQ 60
           MAGEAA +RNVLFGKYEMGRLLGKGTFAKVYYGRNL+THESVAIKVILK+RVKKEGLL+Q
Sbjct: 1   MAGEAAPSRNVLFGKYEMGRLLGKGTFAKVYYGRNLVTHESVAIKVILKERVKKEGLLDQ 60

Query: 61  IRREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYF 120
           I+REISVMGLVRHPHIVELKEVMATKSKVYFVMEYV+GGELFVKLS+QGKLTED ARKYF
Sbjct: 61  IQREISVMGLVRHPHIVELKEVMATKSKVYFVMEYVSGGELFVKLSKQGKLTEDAARKYF 120

Query: 121 QQLISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTP 180
           +QL+SAVDFCHSRGVSHRDLKPENLL+DENGNLKVSDFGLSALPEQLR+DGLLHTRCGTP
Sbjct: 121 RQLVSAVDFCHSRGVSHRDLKPENLLVDENGNLKVSDFGLSALPEQLRVDGLLHTRCGTP 180

Query: 181 AYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWF 240
           AYVAPEVLRK+GYDGAK DIWSCGVIL+VLLAG LPFRADNVMKLYRKIFKA+FEFPPWF
Sbjct: 181 AYVAPEVLRKRGYDGAKVDIWSCGVILYVLLAGALPFRADNVMKLYRKIFKADFEFPPWF 240

Query: 241 SSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELANPKLP 300
           SSE+MEM+SK LVVDP+ R SM EIMQSPWF+ GF+KP+SF EKDEF+NE SEL NPKL 
Sbjct: 241 SSESMEMISKFLVVDPENRVSMPEIMQSPWFRNGFSKPISFSEKDEFNNEISELGNPKL- 300

Query: 301 SSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKAT 360
           SSPPFYNAFEFIS+MSSGFDLSSLFESKKK  SIFTSKCSASAI+AKLQSLA K+NFKAT
Sbjct: 301 SSPPFYNAFEFISTMSSGFDLSSLFESKKKSGSIFTSKCSASAIVAKLQSLAQKVNFKAT 360

Query: 361 NLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPAL 420
           NLKE KVRLQAKEEGRKGKLTVTAEV++ APELAVVEFSKSAGDTLEYNKFCEKDVRPAL
Sbjct: 361 NLKECKVRLQAKEEGRKGKLTVTAEVFEVAPELAVVEFSKSAGDTLEYNKFCEKDVRPAL 420

Query: 421 KDIVWSWQGENECL 435
           KDIVWSWQGE+E L
Sbjct: 421 KDIVWSWQGESEGL 433

BLAST of HG10019487 vs. TAIR 10
Match: AT5G25110.1 (CBL-interacting protein kinase 25 )

HSP 1 Score: 589.7 bits (1519), Expect = 1.9e-168
Identity = 294/430 (68.37%), Postives = 353/430 (82.09%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           VLF KYEMGRLLGKGTF KVYYG+ + T ESVAIK+I KD+VK+EG++EQI+REIS+M L
Sbjct: 38  VLFAKYEMGRLLGKGTFGKVYYGKEITTGESVAIKIINKDQVKREGMMEQIKREISIMRL 97

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAVDFC 130
           VRHP+IVELKEVMATK+K++F+MEYV GGELF K+  +GKL ED ARKYFQQLISAVDFC
Sbjct: 98  VRHPNIVELKEVMATKTKIFFIMEYVKGGELFSKI-VKGKLKEDSARKYFQQLISAVDFC 157

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGVSHRDLKPENLL+DENG+LKVSDFGLSALPEQ+  DGLLHT+CGTPAYVAPEVLRK
Sbjct: 158 HSRGVSHRDLKPENLLVDENGDLKVSDFGLSALPEQILQDGLLHTQCGTPAYVAPEVLRK 217

Query: 191 KGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEMVSK 250
           KGYDGAK DIWSCG+IL+VLLAG LPF+ +N+MK+YRKIFK+EFE+PPWFS E+  ++SK
Sbjct: 218 KGYDGAKGDIWSCGIILYVLLAGFLPFQDENLMKMYRKIFKSEFEYPPWFSPESKRLISK 277

Query: 251 LLVVDPQKRASMVEIMQSPWFQKGFTKPVSF-IEKDEFDNEFSE--------LANPKLPS 310
           LLVVDP KR S+  IM++PWF+K    P+ F I++ E  N   E              P 
Sbjct: 278 LLVVDPNKRISIPAIMRTPWFRKNINSPIEFKIDELEIQNVEDETPTTTATTATTTTTPV 337

Query: 311 SPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKATN 370
           SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+ SAS I+ KL+ +  ++N K   
Sbjct: 338 SPKFFNAFEFISSMSSGFDLSSLFESKRKLRSMFTSRWSASEIMGKLEGIGKEMNMKVKR 397

Query: 371 LKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALK 430
            K+FKV+L  K EGRKG++ VTAEV++ APE+AVVE  KSAGDTLEYN+  E+ VRPAL+
Sbjct: 398 TKDFKVKLFGKTEGRKGQIAVTAEVFEVAPEVAVVELCKSAGDTLEYNRLYEEHVRPALE 457

Query: 431 DIVWSWQGEN 432
           +IVWSW G+N
Sbjct: 458 EIVWSWHGDN 466

BLAST of HG10019487 vs. TAIR 10
Match: AT5G10930.1 (CBL-interacting protein kinase 5 )

HSP 1 Score: 589.3 bits (1518), Expect = 2.4e-168
Identity = 289/434 (66.59%), Postives = 362/434 (83.41%), Query Frame = 0

Query: 9   RNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIRREISV 68
           R VLFGKYEMGRLLGKGTFAKVYYG+ ++  E VAIKVI KD+V K+ G++EQI+REIS+
Sbjct: 5   RRVLFGKYEMGRLLGKGTFAKVYYGKEIIGGECVAIKVINKDQVMKRPGMMEQIKREISI 64

Query: 69  MGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAV 128
           M LVRHP+IVELKEVMATK+K++FVME+V GGELF K+S +GKL ED AR+YFQQLISAV
Sbjct: 65  MKLVRHPNIVELKEVMATKTKIFFVMEFVKGGELFCKIS-KGKLHEDAARRYFQQLISAV 124

Query: 129 DFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEV 188
           D+CHSRGVSHRDLKPENLLLDENG+LK+SDFGLSALPEQ+  DGLLHT+CGTPAYVAPEV
Sbjct: 125 DYCHSRGVSHRDLKPENLLLDENGDLKISDFGLSALPEQILQDGLLHTQCGTPAYVAPEV 184

Query: 189 LRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEM 248
           L+KKGYDGAKADIWSCGV+L+VLLAG LPF+ +N+M +YRKIF+A+FEFPPWFS EA  +
Sbjct: 185 LKKKGYDGAKADIWSCGVVLYVLLAGCLPFQDENLMNMYRKIFRADFEFPPWFSPEARRL 244

Query: 249 VSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSF----------IEKDEFDNEFSELANP 308
           +SKLLVVDP +R S+  IM++PW +K FT P++F           + +E + E  +  N 
Sbjct: 245 ISKLLVVDPDRRISIPAIMRTPWLRKNFTPPLAFKIDEPICSQSSKNNEEEEEDGDCENQ 304

Query: 309 KLPSSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNF 368
             P SP F+NAFEFISSMSSGFDLSSLFESK+K  S+FTS+ SA+ ++ K++++  ++N 
Sbjct: 305 TEPISPKFFNAFEFISSMSSGFDLSSLFESKRKVQSVFTSRSSATEVMEKIETVTKEMNM 364

Query: 369 KATNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVR 428
           K    K+FKV+++ K EGRKG+L++TAEV++ APE++VVEF KSAGDTLEY++  E++VR
Sbjct: 365 KVKRTKDFKVKMEGKTEGRKGRLSMTAEVFEVAPEISVVEFCKSAGDTLEYDRLYEEEVR 424

Query: 429 PALKDIVWSWQGEN 432
           PAL DIVWSW G+N
Sbjct: 425 PALNDIVWSWHGDN 437

BLAST of HG10019487 vs. TAIR 10
Match: AT2G25090.1 (CBL-interacting protein kinase 16 )

HSP 1 Score: 520.0 bits (1338), Expect = 1.8e-147
Identity = 269/446 (60.31%), Postives = 337/446 (75.56%), Query Frame = 0

Query: 4   EAALARNVLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRV-KKEGLLEQIR 63
           E+  +  VLF KY +GRLLG G FAKVY+G  + T + VAIKVI KD V K+ G++EQI 
Sbjct: 3   ESNRSSTVLFDKYNIGRLLGTGNFAKVYHGTEISTGDDVAIKVIKKDHVFKRRGMMEQIE 62

Query: 64  REISVMGLVRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQ 123
           REI+VM L+RHP++VEL+EVMATK K++FVMEYVNGGELF  + + GKL ED ARKYFQQ
Sbjct: 63  REIAVMRLLRHPNVVELREVMATKKKIFFVMEYVNGGELFEMIDRDGKLPEDLARKYFQQ 122

Query: 124 LISAVDFCHSRGVSHRDLKPENLLLDENGNLKVSDFGLSAL--PEQL-----RIDGLLHT 183
           LISAVDFCHSRGV HRD+KPENLLLD  G+LKV+DFGLSAL  PE L       D LLHT
Sbjct: 123 LISAVDFCHSRGVFHRDIKPENLLLDGEGDLKVTDFGLSALMMPEGLGGRRGSSDDLLHT 182

Query: 184 RCGTPAYVAPEVLRKKGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFE 243
           RCGTPAYVAPEVLR KGYDGA ADIWSCG++L+ LLAG LPF  +NVM LY KIFKAE E
Sbjct: 183 RCGTPAYVAPEVLRNKGYDGAMADIWSCGIVLYALLAGFLPFIDENVMTLYTKIFKAECE 242

Query: 244 FPPWFSSEAMEMVSKLLVVDPQKRASMVEIMQSPWFQKGFTKPVSFIEKDEFDNEFSELA 303
           FPPWFS E+ E++S+LLV DP++R SM EI   PWF+K FT  V+F   +   +      
Sbjct: 243 FPPWFSLESKELLSRLLVPDPEQRISMSEIKMIPWFRKNFTPSVAFSIDETIPSPPEPPT 302

Query: 304 NPKLP---------SSPPFYNAFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIA 363
             K           +SP  +NAF+FI+SMSSGFDLS+LFE K+K   +FTSK  A ++  
Sbjct: 303 KKKKKDLNEKEDDGASPRSFNAFQFITSMSSGFDLSNLFEIKRKPKRMFTSKFPAKSVKE 362

Query: 364 KLQSLAVKLNFKATNLKEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTL 423
           +L++ A +++ +  ++K+ K++LQ + EGRKG+L+VTAEV++ APE++VVEF K++GDTL
Sbjct: 363 RLETAAREMDMRVKHVKDCKMKLQRRTEGRKGRLSVTAEVFEVAPEVSVVEFCKTSGDTL 422

Query: 424 EYNKFCEKDVRPALKDIVWSWQGENE 433
           EY  FCE DVRPALKDIVWSWQG+++
Sbjct: 423 EYYLFCEDDVRPALKDIVWSWQGDDD 448

BLAST of HG10019487 vs. TAIR 10
Match: AT5G45820.1 (CBL-interacting protein kinase 20 )

HSP 1 Score: 483.4 bits (1243), Expect = 1.9e-136
Identity = 244/424 (57.55%), Postives = 314/424 (74.06%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           VL  KYE+GRLLG+GTFAKVY+ RN+ T ESVAIKVI K +V K GL++QI+REISVM L
Sbjct: 7   VLMRKYELGRLLGQGTFAKVYHARNIKTGESVAIKVIDKQKVAKVGLIDQIKREISVMRL 66

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAVDFC 130
           VRHPH+V L EVMA+K+K+YF MEYV GGELF K+S +GKL E+ ARKYFQQLI A+D+C
Sbjct: 67  VRHPHVVFLHEVMASKTKIYFAMEYVKGGELFDKVS-KGKLKENIARKYFQQLIGAIDYC 126

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLDENG+LK+SDFGLSAL E  + DGLLHT CGTPAYVAPEV+ K
Sbjct: 127 HSRGVYHRDLKPENLLLDENGDLKISDFGLSALRESKQQDGLLHTTCGTPAYVAPEVIGK 186

Query: 191 KGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEMVSK 250
           KGYDGAKAD+WSCGV+L+VLLAG LPF   N++++YRKI K EF+ P WF  E  +++S+
Sbjct: 187 KGYDGAKADVWSCGVVLYVLLAGFLPFHEQNLVEMYRKITKGEFKCPNWFPPEVKKLLSR 246

Query: 251 LLVVDPQKRASMVEIMQSPWFQKGFTK---PVSFIEKDEFDNEFSELANPKLPSSPPFYN 310
           +L  +P  R  + +IM++ WFQKGF K   P S  E  + D+  S++ +      P  YN
Sbjct: 247 ILDPNPNSRIKIEKIMENSWFQKGFKKIETPKS-PESHQIDSLISDV-HAAFSVKPMSYN 306

Query: 311 AFEFISSMSSGFDLSSLFESKKKRASIFTSKCSASAIIAKLQSLAVKLNFKATNLKEFKV 370
           AF+ ISS+S GFDLS LFE +++  S FT+K  A  I++K + +A           +  V
Sbjct: 307 AFDLISSLSQGFDLSGLFEKEERSESKFTTKKDAKEIVSKFEEIATSSERFNLTKSDVGV 366

Query: 371 RLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKDIVWSW 430
           +++ K EGRKG L +  E+++      +VEF KS GDT+EY +FC++++RP+LKDIVW W
Sbjct: 367 KMEDKREGRKGHLAIDVEIFEVTNSFHMVEFKKSGGDTMEYKQFCDRELRPSLKDIVWKW 426

Query: 431 QGEN 432
           QG N
Sbjct: 427 QGNN 427

BLAST of HG10019487 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 458.0 bits (1177), Expect = 8.5e-129
Identity = 245/424 (57.78%), Postives = 307/424 (72.41%), Query Frame = 0

Query: 11  VLFGKYEMGRLLGKGTFAKVYYGRNLMTHESVAIKVILKDRVKKEGLLEQIRREISVMGL 70
           +L G+YE+GRLLG GTFAKVY+ RN+ T +SVA+KV+ K++V K G+++QI+REISVM +
Sbjct: 19  LLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRM 78

Query: 71  VRHPHIVELKEVMATKSKVYFVMEYVNGGELFVKLSQQGKLTEDGARKYFQQLISAVDFC 130
           V+HP+IVEL EVMA+KSK+YF ME V GGELF K++ +G+L ED AR YFQQLISAVDFC
Sbjct: 79  VKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVA-KGRLREDVARVYFQQLISAVDFC 138

Query: 131 HSRGVSHRDLKPENLLLDENGNLKVSDFGLSALPEQLRIDGLLHTRCGTPAYVAPEVLRK 190
           HSRGV HRDLKPENLLLDE GNLKV+DFGLSA  E L+ DGLLHT CGTPAYVAPEV+ K
Sbjct: 139 HSRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILK 198

Query: 191 KGYDGAKADIWSCGVILFVLLAGTLPFRADNVMKLYRKIFKAEFEFPPWFSSEAMEMVSK 250
           KGYDGAKAD+WSCGVILFVLLAG LPF+ DN++ +YRKI++ +F+ P W SS+A  +V+K
Sbjct: 199 KGYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTK 258

Query: 251 LLVVDPQKRASMVEIMQSPWFQKGFTK----PV-SFIEKDEFDNEFSELANPKLPSSPPF 310
           LL  +P  R ++ ++M SPWF+K  T+    PV + I   E D +F      K       
Sbjct: 259 LLDPNPNTRITIEKVMDSPWFKKQATRSRNEPVAATITTTEEDVDF---LVHKSKEETET 318

Query: 311 YNAFEFISSMSSGFDLSSLFESKK---KRASIFTSKCSASAIIAKLQSLAVKLNFKATNL 370
            NAF  I ++S GFDLS LFE KK   KR   F +   AS++I+ L+  A   N      
Sbjct: 319 LNAFHII-ALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISSLEEAARVGNKFDVRK 378

Query: 371 KEFKVRLQAKEEGRKGKLTVTAEVYKAAPELAVVEFSKSAGDTLEYNKFCEKDVRPALKD 427
            E +VR++ K+ GRKGKL V AE++  AP   VVE  K  GDTLEYN FC   +RPALKD
Sbjct: 379 SESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLEYNNFCSTALRPALKD 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905015.11.3e-23596.31CBL-interacting serine/threonine-protein kinase 25-like [Benincasa hispida][more]
XP_008465153.11.1e-23195.15PREDICTED: CBL-interacting serine/threonine-protein kinase 25-like [Cucumis melo... [more]
XP_004137659.11.8e-22994.69CBL-interacting serine/threonine-protein kinase 25 [Cucumis sativus][more]
KAG6581626.13.5e-22591.49CBL-interacting serine/threonine-protein kinase 25, partial [Cucurbita argyrospe... [more]
XP_022982781.11.3e-22491.72CBL-interacting serine/threonine-protein kinase 25-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8W1D52.6e-16768.37CBL-interacting serine/threonine-protein kinase 25 OS=Arabidopsis thaliana OX=37... [more]
Q9LEU73.4e-16766.59CBL-interacting serine/threonine-protein kinase 5 OS=Arabidopsis thaliana OX=370... [more]
Q9SEZ72.6e-14660.31CBL-interacting serine/threonine-protein kinase 16 OS=Arabidopsis thaliana OX=37... [more]
Q6ERS49.1e-14458.92CBL-interacting protein kinase 16 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9FJ542.7e-13557.55CBL-interacting serine/threonine-protein kinase 20 OS=Arabidopsis thaliana OX=37... [more]
Match NameE-valueIdentityDescription
A0A5A7T8B45.4e-23295.15Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CN525.4e-23295.15Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1J0B36.4e-22591.72Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F2J49.3e-22491.03Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1DJA13.3e-22189.40Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT5G25110.11.9e-16868.37CBL-interacting protein kinase 25 [more]
AT5G10930.12.4e-16866.59CBL-interacting protein kinase 5 [more]
AT2G25090.11.8e-14760.31CBL-interacting protein kinase 16 [more]
AT5G45820.11.9e-13657.55CBL-interacting protein kinase 20 [more]
AT4G30960.18.5e-12957.78SOS3-interacting protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 16..271
e-value: 4.0E-105
score: 365.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 16..271
e-value: 1.4E-76
score: 257.4
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 16..271
score: 52.173553
IPR004041NAF domainPFAMPF03822NAFcoord: 303..359
e-value: 1.6E-16
score: 60.1
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 304..426
e-value: 1.8E-40
score: 139.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 8..303
e-value: 6.5E-105
score: 353.0
NoneNo IPR availablePIRSRPIRSR037993-1PIRSR037993-1coord: 10..274
e-value: 4.6E-44
score: 148.4
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 15..271
e-value: 4.8E-38
score: 128.1
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 15..268
e-value: 5.6E-12
score: 43.1
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 9..433
NoneNo IPR availablePANTHERPTHR43895:SF119CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 25-RELATEDcoord: 9..433
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 307..421
e-value: 5.23471E-43
score: 145.022
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 15..270
e-value: 1.00528E-169
score: 474.2
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 135..147
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 22..54
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 301..326
score: 12.470862
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 12..272

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10019487.1HG10019487.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity