HG10019340 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10019340
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	PMEI domain-containing protein
Location	Chr04: 20545555 .. 20546355 (-)
RNA-Seq Expression	HG10019340
Synteny	HG10019340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGCTCATCAATAATGTCTTCCTCCTCCTCGCCCTCTCTTCTCTTTTCTCCTCTGCCATTGCCATTTGTGTCCCTCGCAACACCTCCGACATTGAATCCATTTCTATTCCACCAGTCGTGCCGCCGAAAACAGCCCCACAGCCATCGTCAGTTACGGTTTCGCCGCCACAATCTCCTACACCACAGGCACCACCGGTTGCAGTTACGGTTTCGCCGCCTCAATCTCCCTCACCACAGACGAATTCCGCTCCAGCGGAGGAGGAATCCGGCTCTGGAGAACACTCAGGACTACCTTTATTTCACAGATTACATGAGAATGATTTGGTATCGTCGGTGGCATCGAGTGTGACGACGGATCTGGTGGAGATCTGCGATGTGACGAGCAATCCTCAGCTATGTAAAACCTCGATTTCATCTCAGGTCCATGGATCGACGATGAATCCTGCAACGGCACTGAAAACAGAAATTGACCAATCGATTAAAGAGGTTTCGAAAGCAATTGCAACATTGAAAACACTTCGTAAAGATTCAGCGGCATCGAAAGCGGAAGTGGCGTGTTACGATACTTGCTTGGAGAATTTCGATATGGCTATTGAAGACCTAAAAGCTGGATTGGAATCGATCGATGTTCATGACGTTGGTAAAATGGAGAGCGTCCTTACGGCCGTATTGACGGATCTGATGACTTGCGATGATACATTTGCAGAGATGGGAGTAGATTCACCATTGGACAATCTTAGCGCGAAGATGAGCAAGTACGCCAGTAACTGCTTGGCGATTTCGAAATTGCTTCTGTAA

mRNA sequence

ATGGAGCTCATCAATAATGTCTTCCTCCTCCTCGCCCTCTCTTCTCTTTTCTCCTCTGCCATTGCCATTTGTGTCCCTCGCAACACCTCCGACATTGAATCCATTTCTATTCCACCAGTCGTGCCGCCGAAAACAGCCCCACAGCCATCGTCAGTTACGGTTTCGCCGCCACAATCTCCTACACCACAGGCACCACCGGTTGCAGTTACGGTTTCGCCGCCTCAATCTCCCTCACCACAGACGAATTCCGCTCCAGCGGAGGAGGAATCCGGCTCTGGAGAACACTCAGGACTACCTTTATTTCACAGATTACATGAGAATGATTTGGTATCGTCGGTGGCATCGAGTGTGACGACGGATCTGGTGGAGATCTGCGATGTGACGAGCAATCCTCAGCTATGTAAAACCTCGATTTCATCTCAGGTCCATGGATCGACGATGAATCCTGCAACGGCACTGAAAACAGAAATTGACCAATCGATTAAAGAGGTTTCGAAAGCAATTGCAACATTGAAAACACTTCGTAAAGATTCAGCGGCATCGAAAGCGGAAGTGGCGTGTTACGATACTTGCTTGGAGAATTTCGATATGGCTATTGAAGACCTAAAAGCTGGATTGGAATCGATCGATGTTCATGACGTTGGTAAAATGGAGAGCGTCCTTACGGCCGTATTGACGGATCTGATGACTTGCGATGATACATTTGCAGAGATGGGAGTAGATTCACCATTGGACAATCTTAGCGCGAAGATGAGCAAGTACGCCAGTAACTGCTTGGCGATTTCGAAATTGCTTCTGTAA

Coding sequence (CDS)

ATGGAGCTCATCAATAATGTCTTCCTCCTCCTCGCCCTCTCTTCTCTTTTCTCCTCTGCCATTGCCATTTGTGTCCCTCGCAACACCTCCGACATTGAATCCATTTCTATTCCACCAGTCGTGCCGCCGAAAACAGCCCCACAGCCATCGTCAGTTACGGTTTCGCCGCCACAATCTCCTACACCACAGGCACCACCGGTTGCAGTTACGGTTTCGCCGCCTCAATCTCCCTCACCACAGACGAATTCCGCTCCAGCGGAGGAGGAATCCGGCTCTGGAGAACACTCAGGACTACCTTTATTTCACAGATTACATGAGAATGATTTGGTATCGTCGGTGGCATCGAGTGTGACGACGGATCTGGTGGAGATCTGCGATGTGACGAGCAATCCTCAGCTATGTAAAACCTCGATTTCATCTCAGGTCCATGGATCGACGATGAATCCTGCAACGGCACTGAAAACAGAAATTGACCAATCGATTAAAGAGGTTTCGAAAGCAATTGCAACATTGAAAACACTTCGTAAAGATTCAGCGGCATCGAAAGCGGAAGTGGCGTGTTACGATACTTGCTTGGAGAATTTCGATATGGCTATTGAAGACCTAAAAGCTGGATTGGAATCGATCGATGTTCATGACGTTGGTAAAATGGAGAGCGTCCTTACGGCCGTATTGACGGATCTGATGACTTGCGATGATACATTTGCAGAGATGGGAGTAGATTCACCATTGGACAATCTTAGCGCGAAGATGAGCAAGTACGCCAGTAACTGCTTGGCGATTTCGAAATTGCTTCTGTAA

Protein sequence

MELINNVFLLLALSSLFSSAIAICVPRNTSDIESISIPPVVPPKTAPQPSSVTVSPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGSGEHSGLPLFHRLHENDLVSSVASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL

Homology

[Close]

BLAST of HG10019340 vs. NCBI nr
Match: XP_038903716.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 372.9 bits (956), Expect = 2.4e-99
Identity = 217/286 (75.87%), Postives = 235/286 (82.17%), Query Frame = 0


Query: 1   MELINNVFLLLALSSLFSSAIAICVPRNTSDIESISIPPVVPPKTAPQ------------ 60
           MELINNVFLLLA SSLFSSAIAICVPRN     S SIPP + P+ APQ            
Sbjct: 1   MELINNVFLLLAFSSLFSSAIAICVPRNALGFVSTSIPPTIEPQIAPQTTESVTASLPQS 60

Query: 61  PSS------VTVSPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEE--ESGSGEHSGLPL 120
           PS+      VTVSPPQSP  QAPP  VTVSPPQSPSP TNSAPAEE  ES S E SGLP 
Sbjct: 61  PSAQTPQVEVTVSPPQSPPTQAPPATVTVSPPQSPSPSTNSAPAEEEQESASEEDSGLPE 120

Query: 121 FHRLHENDLVSSVASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQS 180
           F +L  ++LVSSVASS TTDLVEICDVTSNPQLCKTSISSQ+HG T++P +ALKTEI QS
Sbjct: 121 FQKLPSDNLVSSVASSTTTDLVEICDVTSNPQLCKTSISSQIHGMTVDPTSALKTEIQQS 180

Query: 181 IKEVSKAIATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESV 240
           +KEV+KAIATLK LRKD +AS  E+ACYDTCLENFD+AIEDLKAGLESIDVHDVG+MESV
Sbjct: 181 MKEVTKAIATLKELRKDPSASNVEIACYDTCLENFDIAIEDLKAGLESIDVHDVGRMESV 240

Query: 241 LTAVLTDLMTCDDTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
           LTAVLTDLMTCDDTFAEMGVDSPLD+LS +MSKYASNCLAISKLLL
Sbjct: 241 LTAVLTDLMTCDDTFAEMGVDSPLDSLSTRMSKYASNCLAISKLLL 286

[Close]

BLAST of HG10019340 vs. NCBI nr
Match: XP_008454280.1 (PREDICTED: pectinesterase inhibitor 1 [Cucumis melo] >KAA0044419.1 pectinesterase inhibitor 1 [Cucumis melo var. makuwa] >TYK29546.1 pectinesterase inhibitor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 301.6 bits (771), Expect = 6.7e-78
Identity = 177/267 (66.29%), Postives = 211/267 (79.03%), Query Frame = 0


Query: 4   INNVFLLLALSSLFSSAIAICVPR--NTSDIESISIPPVVPPKTAPQPSSVTVSPPQSPT 63
           INNVFLLLA SS+F SAIAICVP   N +D+ES +IPP++    +PQ     V+P QSP 
Sbjct: 8   INNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQ-----VAPDQSP- 67

Query: 64  PQAPPVAVTVSPPQSPSPQTNSA--PAEEESGSGEHSGLPLFHRLHENDLVSSVASSVTT 123
              P V     PP++ SPQT+++   +  E    E+SGL     LH + L+S+ AS+V T
Sbjct: 68  ---PAVTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLHSDGLISTAASTVKT 127

Query: 124 DLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTLRKDSA 183
           +LVEICDVTSNPQLCKTSISS + G+ ++PA+ALKTEI++SIKEV+KAIATL +LRKDS+
Sbjct: 128 NLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIATLNSLRKDSS 187

Query: 184 ASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDTFAEMG 243
           AS+ E+ACYDTCLENFDMAIEDLKAG+ESID  D G+MESVLTAVLTDL TCDDTFAEMG
Sbjct: 188 ASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTTCDDTFAEMG 247

Query: 244 VDSPLDNLSAKMSKYASNCLAISKLLL 267
           VDSPLDNLS KMSKYASNCLAISKLLL
Sbjct: 248 VDSPLDNLSTKMSKYASNCLAISKLLL 265

[Close]

BLAST of HG10019340 vs. NCBI nr
Match: XP_011654315.2 (uncharacterized protein LOC105435336 [Cucumis sativus] >KAE8649055.1 hypothetical protein Csa_015367 [Cucumis sativus])

HSP 1 Score: 297.0 bits (759), Expect = 1.7e-76
Identity = 185/285 (64.91%), Postives = 218/285 (76.49%), Query Frame = 0


Query: 1   MELIN----NVFLLLALSSLFSSAIAICVPRNTSDIESISIPPV--------VPPKTAPQ 60
           MELIN    NVFLLLA SS+FS A+A+CVPRNTSDI S +IPP+        V P+ APQ
Sbjct: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60

Query: 61  -PSSVTVS-PPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGS-GEHSGLPL----F 120
            P +VTVS  P++ +P+  P     S P S S    +A   EE+ S  E   L L     
Sbjct: 61  SPPAVTVSEEPEAASPETSP-----SQPSSASESEEAASESEEAASETEEDDLGLLGGGL 120

Query: 121 HRLHENDLVSSVASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSI 180
            +LH  DL+SSV SSVTT LV+ICDVTSNPQLCKTSISS + G+ ++PA+ALKTEID+SI
Sbjct: 121 DQLHSADLISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIDESI 180

Query: 181 KEVSKAIATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVL 240
           KEV+KAIATL +LRKDSAAS+ E+ACYDTCLENF+MAI+DLKAG+ESI+  D G+MESVL
Sbjct: 181 KEVAKAIATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVL 240

Query: 241 TAVLTDLMTCDDTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
           TAV+TDL TCDDTFAEMGVDSPLD+LS KMSKYASNCLAISKLLL
Sbjct: 241 TAVMTDLTTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 280

[Close]

BLAST of HG10019340 vs. NCBI nr
Match: XP_022983975.1 (uncharacterized protein LOC111482436 [Cucurbita maxima])

HSP 1 Score: 241.1 bits (614), Expect = 1.1e-59
Identity = 155/274 (56.57%), Postives = 184/274 (67.15%), Query Frame = 0


Query: 1   MELINNVF-------LLLALSSLFSS-AIAICVPRNTSDIESISIPPVVPPKTAPQPSSV 60
           MELINNVF       LLLALSSLFSS AIAICVPRN S  +S +IP              
Sbjct: 32  MELINNVFHLHLHLHLLLALSSLFSSPAIAICVPRNISLFQSPTIP-------------- 91

Query: 61  TVSPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGSGEHSGLPLFHRLHENDLVSS 120
                  PTP  PPV ++ S  +    +T  +  +  S SG                   
Sbjct: 92  ------LPTPSTPPVYISQSVARK---RTQKSEGKSSSKSG------------------- 151

Query: 121 VASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLK 180
            +S    +LV+ICDVTSNP+LC++SISS +H ST+NP +ALKTEI+QSIKEV  AIA LK
Sbjct: 152 -SSREPIELVQICDVTSNPKLCQSSISSSIHESTVNPVSALKTEIEQSIKEVENAIAVLK 211

Query: 181 TLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCD 240
            L K+S ++KAE +CYDTCLENFDMAIEDL+   E+IDV+DVG+MES LTAVLTDL+TCD
Sbjct: 212 KLDKESGSTKAEDSCYDTCLENFDMAIEDLRTATEAIDVNDVGRMESALTAVLTDLVTCD 262

Query: 241 DTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
           DTF EMGVDSPLD+LS KM+KYASNCLAISKLLL
Sbjct: 272 DTFVEMGVDSPLDHLSIKMNKYASNCLAISKLLL 262

[Close]

BLAST of HG10019340 vs. NCBI nr
Match: KAG7018024.1 (hypothetical protein SDJN02_19890, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 237.7 bits (605), Expect = 1.2e-58
Identity = 150/274 (54.74%), Postives = 184/274 (67.15%), Query Frame = 0


Query: 1   MELINNVF-------LLLALSSLFSS-AIAICVPRNTSDIESISIPPVVPPKTAPQPSSV 60
           ME+INNVF       LLLALSSLFSS AIAICVPRNTS  +  ++P              
Sbjct: 1   MEVINNVFHRLLLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLP-------------- 60

Query: 61  TVSPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGSGEHSGLPLFHRLHENDLVSS 120
                  PTP  PPV ++ S  +  +P++    + + + S                    
Sbjct: 61  ------LPTPSTPPVYISQSISRKRTPKSAGKSSSKSASS-------------------- 120

Query: 121 VASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLK 180
            +S+  T+LV+ICDVTSNP+LC++SISS +HGST+NP +ALKTEI  SIKEV  AIA LK
Sbjct: 121 -SSTEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLK 180

Query: 181 TLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCD 240
            L K+S  + AE +CYDTCLENFDMAI+DL+   E+IDV+DVG+MES LTAVLTDL+TCD
Sbjct: 181 KLDKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDLVTCD 233

Query: 241 DTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
           DTF EMGVDSPLD+LS KM+KYASNCLAISKLLL
Sbjct: 241 DTFLEMGVDSPLDDLSIKMNKYASNCLAISKLLL 233

[Close]

BLAST of HG10019340 vs. ExPASy TrEMBL
Match: A0A5A7TT72 (Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G001500 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 3.2e-78
Identity = 177/267 (66.29%), Postives = 211/267 (79.03%), Query Frame = 0


Query: 4   INNVFLLLALSSLFSSAIAICVPR--NTSDIESISIPPVVPPKTAPQPSSVTVSPPQSPT 63
           INNVFLLLA SS+F SAIAICVP   N +D+ES +IPP++    +PQ     V+P QSP 
Sbjct: 8   INNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQ-----VAPDQSP- 67

Query: 64  PQAPPVAVTVSPPQSPSPQTNSA--PAEEESGSGEHSGLPLFHRLHENDLVSSVASSVTT 123
              P V     PP++ SPQT+++   +  E    E+SGL     LH + L+S+ AS+V T
Sbjct: 68  ---PAVTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLHSDGLISTAASTVKT 127

Query: 124 DLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTLRKDSA 183
           +LVEICDVTSNPQLCKTSISS + G+ ++PA+ALKTEI++SIKEV+KAIATL +LRKDS+
Sbjct: 128 NLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIATLNSLRKDSS 187

Query: 184 ASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDTFAEMG 243
           AS+ E+ACYDTCLENFDMAIEDLKAG+ESID  D G+MESVLTAVLTDL TCDDTFAEMG
Sbjct: 188 ASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTTCDDTFAEMG 247

Query: 244 VDSPLDNLSAKMSKYASNCLAISKLLL 267
           VDSPLDNLS KMSKYASNCLAISKLLL
Sbjct: 248 VDSPLDNLSTKMSKYASNCLAISKLLL 265

[Close]

BLAST of HG10019340 vs. ExPASy TrEMBL
Match: A0A1S3BYC7 (pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 3.2e-78
Identity = 177/267 (66.29%), Postives = 211/267 (79.03%), Query Frame = 0


Query: 4   INNVFLLLALSSLFSSAIAICVPR--NTSDIESISIPPVVPPKTAPQPSSVTVSPPQSPT 63
           INNVFLLLA SS+F SAIAICVP   N +D+ES +IPP++    +PQ     V+P QSP 
Sbjct: 8   INNVFLLLAFSSVFFSAIAICVPLNINITDVESTAIPPLLEQTVSPQ-----VAPDQSP- 67

Query: 64  PQAPPVAVTVSPPQSPSPQTNSA--PAEEESGSGEHSGLPLFHRLHENDLVSSVASSVTT 123
              P V     PP++ SPQT+++   +  E    E+SGL     LH + L+S+ AS+V T
Sbjct: 68  ---PAVTTVSQPPEATSPQTSTSDQSSSNEISDEENSGLLGLDYLHSDGLISTAASTVKT 127

Query: 124 DLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTLRKDSA 183
           +LVEICDVTSNPQLCKTSISS + G+ ++PA+ALKTEI++SIKEV+KAIATL +LRKDS+
Sbjct: 128 NLVEICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIEESIKEVTKAIATLNSLRKDSS 187

Query: 184 ASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDTFAEMG 243
           AS+ E+ACYDTCLENFDMAIEDLKAG+ESID  D G+MESVLTAVLTDL TCDDTFAEMG
Sbjct: 188 ASETEIACYDTCLENFDMAIEDLKAGVESIDARDAGRMESVLTAVLTDLTTCDDTFAEMG 247

Query: 244 VDSPLDNLSAKMSKYASNCLAISKLLL 267
           VDSPLDNLS KMSKYASNCLAISKLLL
Sbjct: 248 VDSPLDNLSTKMSKYASNCLAISKLLL 265

[Close]

BLAST of HG10019340 vs. ExPASy TrEMBL
Match: A0A0A0KWP8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 3.6e-77
Identity = 178/278 (64.03%), Postives = 208/278 (74.82%), Query Frame = 0


Query: 1   MELIN----NVFLLLALSSLFSSAIAICVPRNTSDIESISIPPVVPPKTAPQ--PSSVTV 60
           MELIN    NVFLLLA SS+FS A+A+CVPRNTSDI S +IPP+     +PQ  P     
Sbjct: 1   MELINNSIHNVFLLLAFSSVFSFAVAVCVPRNTSDIGSTAIPPLFEQTVSPQVAPQVAPQ 60

Query: 61  SPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGSGEHSGLPL------FHRLHEND 120
           SPP     + P  A   + P  PS  + S  AE E  + E     L        +LH  D
Sbjct: 61  SPPAVTVSEEPEAASPETSPSQPSSASESEEAESEEAASETEEDDLGLLGGGLDQLHSAD 120

Query: 121 LVSSVASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAI 180
           L+SSV SSVTT LV+ICDVTSNPQLCKTSISS + G+ ++PA+ALKTEID+SIKEV+KAI
Sbjct: 121 LISSVTSSVTTSLVDICDVTSNPQLCKTSISSHIEGTKVDPASALKTEIDESIKEVAKAI 180

Query: 181 ATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDL 240
           ATL +LRKDSAAS+ E+ACYDTCLENF+MAI+DLKAG+ESI+  D G+MESVLTAV+TDL
Sbjct: 181 ATLNSLRKDSAASETEIACYDTCLENFEMAIDDLKAGVESINARDAGRMESVLTAVMTDL 240

Query: 241 MTCDDTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
            TCDDTFAEMGVDSPLD+LS KMSKYASNCLAISKLLL
Sbjct: 241 TTCDDTFAEMGVDSPLDSLSTKMSKYASNCLAISKLLL 278

[Close]

BLAST of HG10019340 vs. ExPASy TrEMBL
Match: A0A6J1J7E6 (uncharacterized protein LOC111482436 OS=Cucurbita maxima OX=3661 GN=LOC111482436 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 5.2e-60
Identity = 155/274 (56.57%), Postives = 184/274 (67.15%), Query Frame = 0


Query: 1   MELINNVF-------LLLALSSLFSS-AIAICVPRNTSDIESISIPPVVPPKTAPQPSSV 60
           MELINNVF       LLLALSSLFSS AIAICVPRN S  +S +IP              
Sbjct: 32  MELINNVFHLHLHLHLLLALSSLFSSPAIAICVPRNISLFQSPTIP-------------- 91

Query: 61  TVSPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGSGEHSGLPLFHRLHENDLVSS 120
                  PTP  PPV ++ S  +    +T  +  +  S SG                   
Sbjct: 92  ------LPTPSTPPVYISQSVARK---RTQKSEGKSSSKSG------------------- 151

Query: 121 VASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLK 180
            +S    +LV+ICDVTSNP+LC++SISS +H ST+NP +ALKTEI+QSIKEV  AIA LK
Sbjct: 152 -SSREPIELVQICDVTSNPKLCQSSISSSIHESTVNPVSALKTEIEQSIKEVENAIAVLK 211

Query: 181 TLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCD 240
            L K+S ++KAE +CYDTCLENFDMAIEDL+   E+IDV+DVG+MES LTAVLTDL+TCD
Sbjct: 212 KLDKESGSTKAEDSCYDTCLENFDMAIEDLRTATEAIDVNDVGRMESALTAVLTDLVTCD 262

Query: 241 DTFAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
           DTF EMGVDSPLD+LS KM+KYASNCLAISKLLL
Sbjct: 272 DTFVEMGVDSPLDHLSIKMNKYASNCLAISKLLL 262

[Close]

BLAST of HG10019340 vs. ExPASy TrEMBL
Match: A0A6J1F7J2 (uncharacterized protein LOC111441579 OS=Cucurbita moschata OX=3662 GN=LOC111441579 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 1.7e-58
Identity = 149/272 (54.78%), Postives = 183/272 (67.28%), Query Frame = 0


Query: 1   MELINNVF-----LLLALSSLFSS-AIAICVPRNTSDIESISIPPVVPPKTAPQPSSVTV 60
           ME+INNVF     LLLALSSLFSS AIAICVPRNTS  +  ++P                
Sbjct: 1   MEVINNVFHRLLLLLLALSSLFSSPAIAICVPRNTSLFQFSTLP---------------- 60

Query: 61  SPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGSGEHSGLPLFHRLHENDLVSSVA 120
                PTP  PPV ++ S  +  +P++    + + + S                     +
Sbjct: 61  ----LPTPSTPPVYISQSISRKRTPKSAGKSSSKSASS---------------------S 120

Query: 121 SSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTL 180
           S+  T+LV+ICDVTSNP+LC++SISS +HGST+NP +ALKTEI  SIKEV  AIA LK L
Sbjct: 121 STEPTELVQICDVTSNPKLCESSISSSIHGSTVNPVSALKTEIQLSIKEVEDAIAVLKKL 180

Query: 181 RKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDT 240
            K+S  + AE +CYDTCLENFDMAI+DL+   E+IDV+DVG+MES LTAVLTDL+TCDDT
Sbjct: 181 DKESGLTNAEDSCYDTCLENFDMAIDDLRTATEAIDVNDVGRMESALTAVLTDLVTCDDT 231

Query: 241 FAEMGVDSPLDNLSAKMSKYASNCLAISKLLL 267
           F EMGVDSPLD+LS KM+KYA NCLAISKLLL
Sbjct: 241 FLEMGVDSPLDDLSIKMNKYAINCLAISKLLL 231

[Close]

BLAST of HG10019340 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.1e-09
Identity = 76/277 (27.44%), Postives = 123/277 (44.40%), Query Frame = 0


Query: 16  LFSSAIAICVPRNTSDIESISIPPVVPPKTAPQPSSVTVSPPQSP--------------- 75
           +F+ +I + +   ++ +ES S  P   P  AP+      SP  SP               
Sbjct: 12  IFTVSILVTI---STHVESRSDSPFSTPAPAPEVMKPIASPANSPAEIYTDSPVASPEAP 71

Query: 76  ----TPQAPPVAVTV-SPPQSPSPQTNSAPAEEESGSGEHSGLPLFHRLHENDLVSSVAS 135
               +P+ PPV + + SP  SP     S  A  ES S   SG+           VSS   
Sbjct: 72  ADSFSPKNPPVEIDIYSPAASPEAPVASPEAPAESSSAAPSGIK----------VSSSPK 131

Query: 136 S------VTTDLVEICDVTSNPQLCKTSISSQVHGSTM-NPATALKTEIDQSIKEVSKAI 195
           S      ++ ++  IC  T NP LC++S+S  +      N ++ L   I  SI     A+
Sbjct: 132 SLLNPPLLSPEIKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAM 191

Query: 196 ATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDL 255
           A ++ +                C E +D A+ +L+  + ++   D+  + + L+A +TD 
Sbjct: 192 AIVEKVDASD------------CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDY 251

Query: 256 MTCDDTFAEMGVDSPLDNLSAKMSKYASNCLAISKLL 266
            TC+D F E G  +PL  ++ K++K  SNCLAIS L+
Sbjct: 252 STCNDGFEESGEPNPLAYVADKLTKMVSNCLAISTLI 263

[Close]

BLAST of HG10019340 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 7.1e-09
Identity = 46/157 (29.30%), Postives = 79/157 (50.32%), Query Frame = 0


Query: 109 LVSSVASSVTTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAI 168
           L ++ + +++  L + C V+  P LC+++I  Q   + +N AT      D +IKE+ K  
Sbjct: 13  LAATSSQALSISLADPCTVSDFPALCRSTIKGQ---TNVNAAT------DVAIKELMKRT 72

Query: 169 ATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDL 228
              K + +        VA   TCL NF+ A ++L+  L +I  +D   +   L+A LTD 
Sbjct: 73  RQAKKIAEKELKRDGGVA---TCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDY 132

Query: 229 MTCDDTFAEMGVDSPLDNLSAKMSKYASNCLAISKLL 266
            TC D+  E    + +   +  + + A NCLA+S L+
Sbjct: 133 DTCSDSMKETKEVNVIYKSAGVLYRMADNCLALSTLV 157

[Close]

BLAST of HG10019340 vs. TAIR 10
Match: AT2G47340.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.3e-07
Identity = 65/250 (26.00%), Postives = 113/250 (45.20%), Query Frame = 0


Query: 25  VPRNTSDIESISIPPV----VPPKTAPQPSSVTVSPPQSPTPQAPPVAVTVSPPQSPSPQ 84
           VP ++ D  S+  PPV    V P +AP P+S  ++   + +  AP  AV+     +P P 
Sbjct: 38  VPASSPD-PSLIEPPVSSISVVPASAPSPTSEDIN---TGSNLAPTAAVS-----APGPS 97

Query: 85  TNSAPAEEESGSGEHSGLPLFHRLHENDLVSSVASSVTTDLVEICDVTSN------PQLC 144
                   +S +      P F  +++ D  S    +   DL +  D  +       P L 
Sbjct: 98  QEDIDINFDSVTNIADLAPKFEHINKFDFSSMKIDTTAKDLCKNTDYNNECIAAILPDLQ 157

Query: 145 KTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTLRKDSAASKAEVACYDTCLEN 204
           K +      G  + P   ++ E +  +K+ +  +   K + +D             C+EN
Sbjct: 158 KQAEGEGGDGG-IAPKDVMRMEAESLLKKANATLDYAKRVVEDRKTPMPVKEAMSVCVEN 217

Query: 205 FDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDTFAEM-GVDSPLDNLSAKMSK 264
           +D  I  L+    +++  D G+++SVL+A ++D+ TC D F ++  VDSP  +L   M K
Sbjct: 218 YDSLISGLEDAKMAMEEGDYGRLDSVLSAAISDVSTCSDNFVDIPDVDSPTASLDDLMKK 277

[Close]

BLAST of HG10019340 vs. TAIR 10
Match: AT1G02550.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.1e-06
Identity = 58/229 (25.33%), Postives = 102/229 (44.54%), Query Frame = 0


Query: 54  VSPPQSPTPQAPPVAVTVSPPQSPSPQTNSAPAEEESGS-------------GEHSGLPL 113
           +S P S T     +  + +P  + +P T+    E +  +                  LP 
Sbjct: 18  LSSPSSSTRIISSIVPSAAPSPAVAPTTDGDVDENDFSAFTQWNILNLTDLKSTFKNLPD 77

Query: 114 FHRLHENDL-VSSVASSV--TTDLVEICDVTSNPQLCKTSISSQVHGSTMNPATALKTEI 173
           F +L+ + L VS    SV   TD    C V+  P L               P   L+ E+
Sbjct: 78  FSKLNISSLHVSPAVGSVCSNTDYAAECIVSILPLL------RDFRKFEPKPIDVLRMEM 137

Query: 174 DQSIKEVSKAIATLKTLRKDSAASKAEVACYDTCLENFDMAIEDLKAGLESIDVHDVGKM 233
               ++ +  +   K L  D +  +      D C++N++  ++DLK    ++D  D  ++
Sbjct: 138 SALYEKANATLDLAKRLIVDKSTPRDVADVLDLCVDNYESLLDDLKDASVAVDDGDFERL 197

Query: 234 ESVLTAVLTDLMTCDDTFAEMG-VDSPLDNLSAKMSKYASNCLAISKLL 266
           ESV++A + D++TC D FAE   ++SP+ N+   + K  SN LAIS+++
Sbjct: 198 ESVVSAAIADVVTCSDAFAESSELESPMANVDDFLKKLCSNVLAISQMI 240

[Close]

BLAST of HG10019340 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.5e-06
Identity = 40/141 (28.37%), Postives = 64/141 (45.39%), Query Frame = 0


Query: 125 CDVTSNPQLCKTSISSQVHGSTMNPATALKTEIDQSIKEVSKAIATLKTLRKDSAASKAE 184
           C V+    LC++ +  Q      N   A +  I + +K   KA    K  RK     K  
Sbjct: 37  CSVSDFKVLCRSVVKGQ-----KNVNAATEVSIRELMKRTIKAKEAAKISRKSGGGLK-- 96

Query: 185 VACYDTCLENFDMAIEDLKAGLESIDVHDVGKMESVLTAVLTDLMTCDDTFAEMGVDSPL 244
                TC  N+D A+E+L+  L++I  +D   +   L+A LTD  TC+D        +  
Sbjct: 97  -----TCYSNYDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGGGTASNVF 156

Query: 245 DNLSAKMSKYASNCLAISKLL 266
              ++ + + A NCLA+S L+
Sbjct: 157 AKSTSTLHEMADNCLALSTLV 165

The following BLAST results are available for this feature:

BLAST of HG10019340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903716.1	2.4e-99	75.87	21 kDa protein-like [Benincasa hispida]	[more]
XP_008454280.1	6.7e-78	66.29	PREDICTED: pectinesterase inhibitor 1 [Cucumis melo] >KAA0044419.1 pectinesteras...	[more]
XP_011654315.2	1.7e-76	64.91	uncharacterized protein LOC105435336 [Cucumis sativus] >KAE8649055.1 hypothetica...	[more]
XP_022983975.1	1.1e-59	56.57	uncharacterized protein LOC111482436 [Cucurbita maxima]	[more]
KAG7018024.1	1.2e-58	54.74	hypothetical protein SDJN02_19890, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of HG10019340 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of HG10019340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TT72	3.2e-78	66.29	Pectinesterase inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3BYC7	3.2e-78	66.29	pectinesterase inhibitor 1 OS=Cucumis melo OX=3656 GN=LOC103494726 PE=4 SV=1	[more]
A0A0A0KWP8	3.6e-77	64.03	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G003750 PE=4 S...	[more]
A0A6J1J7E6	5.2e-60	56.57	uncharacterized protein LOC111482436 OS=Cucurbita maxima OX=3661 GN=LOC111482436...	[more]
A0A6J1F7J2	1.7e-58	54.78	uncharacterized protein LOC111441579 OS=Cucurbita moschata OX=3662 GN=LOC1114415...	[more]

BLAST of HG10019340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G36659.1	1.1e-09	27.44	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G23350.1	7.1e-09	29.30	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47340.1	1.3e-07	26.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G02550.1	1.1e-06	25.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70540.1	1.5e-06	28.37	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 116..261 e-value: 7.9E-25 score: 98.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 123..261 e-value: 6.8E-20 score: 71.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 117..265 e-value: 2.3E-23 score: 80.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 121..265 e-value: 9.5E-29 score: 102.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 123..265
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 74..89
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 34..99
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 40..73
None	No IPR available	PANTHER	PTHR31707:SF119	INVERTASE-RELATED	coord: 10..260
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 10..260
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 123..265 e-value: 2.28452E-48 score: 155.596

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10019340.1	HG10019340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity