HG10018893 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10018893
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionRNase H domain-containing protein
LocationChr04: 10684453 .. 10686144 (-)
RNA-Seq ExpressionHG10018893
SyntenyHG10018893
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTTAAAACTAAACATTGATGCTTCGCGTGTTGATCCATCAAGCATCGTGGGTCTGGGATATGTCATTCAAAATTCCAAAGGAGAGGTGTTGGTGGTTGGGGCCGAGTTTTTGGAAAGCACATGTGAGGTTGTGGATGCTGAAGCAATGACCCTTCTTGTTGGGCTCAAGATTGTGGGTGATATGAACATTCAAAATGTTTGGGTCGAATCTAATTCTCAAATCCTGGTTAAAGCTATCCACGATCATTCTATGATACATTCCCGAATTGGGGTTATGTTGGAAGAGATTAGAGAGTGTATGTTTCGTATTCACATTTTTAGGTTATCTTTCATTCCCTGATCTTGTAATTGGGTAGCTCATAAGTTTGTTGGGTTGGCTATCACCAATGTTGATGGTGGGATGTGGACTACTAACATGCCTCATTGGTTAAATTTTTTAATTGAGTATAATGCTCATGGATGTAACCCGTAATGGGATGCATTTTGTTAATAAAATTGGTGATTATAAAAAAAAAAAAAACTCCATGATGTGCATTTTGTTATGATTTAACCAAAAAAAAATGGGAATATTAGAAAATTGATTTCTTTAAGGGGTTGTTTGATGCGCTGAGTTGAGTTCATATGTTCGAAGTTCATATGTCTGTGAAGTTTATATGTTTATGGAGTTCATATGTCTGTGTTTTTAGTATTTGGGGTGCTAAGTTCATATGTTAGGGGTATCTCGTATAATTTTTTTAACATAAATAATATGTTTTTATGATGATTATTTTTGTTTGGAAACTTTTTTATTCAATAATTCTACATATATTTTTTTAATAAGCTATAGAGTTGCATTTTTTTAATTATTTTTTAAACATACATGTATTGTTGGGAGATTTGGGAAATTGGGTTTAGGGTTTACTTTTGGATCTTCAATTTTTTTTTTAAATTTAATCAATCATTTTAAAAATTTGTGCATGTGACGTTTCTAGTTAAGTCATTTGTATTTTCACTTTTGATAAAAACTTTTTTAAAATAAAAACATTCATGTGTCTGACAAATTTTCTCTAAAGTGTTTTTACATACAAGTTTGTTTGATTTCTTACATGATAAAAGTAATTTTATTAATGTTAAATTACGGTAAAGAACGACTATAAAATACTATGAACTAATAAAAAAATGAAAATTCAACGTTTTAACGAAACTTACGTACTTTTGTGTTAACAAATGTGTTTTTATTGACAATTTGCGTCAATTTAGATATAAACATTTATTGATATTTTATATGTATTTTTTAATTAATACTTATATTTAAGCTTTAAATTAAGGTTTTATTGACGGTTTTATGTCATGAGCATCAACAAAACAAAAAAAATTATGTGTCAACAAAGCCCAAATTTGTAGCAGCCATAATTTAAAATAATGGTCGTCGGAGTTGGTGTAGTGACCCCCATTCGAGGTTGTTGGAGTTCGTCGTCAACAATGATAGTTAGATGTTGCTGGAGTTGGCCACAGGCGATGGTTGTCGAAGGTGGTCATCGGAGTTGGTTGTCAGAAGTGGTCTCCGGAGTTGGATGTCGGAGGTGGTCGCCTGAGTTTGGTCGTCAAATGTGGTGTCGGAGTTAGCCGTAGAAGGTTTCATCGAAGTTGGTCGTTAGAGGTGGTCACCGAAGTTGAGCAACGAGACATCTGTGGTGATGAGGGTTGA

mRNA sequence

ATGAAGTTAAAACTAAACATTGATGCTTCGCGTGTTGATCCATCAAGCATCGTGGGTCTGGGATATGTCATTCAAAATTCCAAAGGAGAGGTGTTGGTGGTTGGGGCCGAGTTTTTGGAAAGCACATGTGAGGTTGTGGATGCTGAAGCAATGACCCTTCTTGTTGGGCTCAAGATTGTGGGTGATATGAACATTCAAAATGTTTGGGTCGAATCTAATTCTCAAATCCTGGTTAAAGCTATCCACGATCATTCTATGATACATTCCCGAATTGGGGTTATGTTGGAAGAGATTAGAGAGTATGTTGCTGGAGTTGGCCACAGGCGATGGTTGTCGAAGGTGGTCATCGGAGTTGGTTGTCAGAAGTGGTCTCCGGAGTTGGATGTCGGAGGTGGTCGCCTGAGTTTGGTCGTCAAATGTGGTGTCGGAGTTAGCCGTAGAAGGTTTCATCGAAGTTGGTCGTTAGAGGTGGTCACCGAAGTTGAGCAACGAGACATCTGTGGTGATGAGGGTTGA

Coding sequence (CDS)

ATGAAGTTAAAACTAAACATTGATGCTTCGCGTGTTGATCCATCAAGCATCGTGGGTCTGGGATATGTCATTCAAAATTCCAAAGGAGAGGTGTTGGTGGTTGGGGCCGAGTTTTTGGAAAGCACATGTGAGGTTGTGGATGCTGAAGCAATGACCCTTCTTGTTGGGCTCAAGATTGTGGGTGATATGAACATTCAAAATGTTTGGGTCGAATCTAATTCTCAAATCCTGGTTAAAGCTATCCACGATCATTCTATGATACATTCCCGAATTGGGGTTATGTTGGAAGAGATTAGAGAGTATGTTGCTGGAGTTGGCCACAGGCGATGGTTGTCGAAGGTGGTCATCGGAGTTGGTTGTCAGAAGTGGTCTCCGGAGTTGGATGTCGGAGGTGGTCGCCTGAGTTTGGTCGTCAAATGTGGTGTCGGAGTTAGCCGTAGAAGGTTTCATCGAAGTTGGTCGTTAGAGGTGGTCACCGAAGTTGAGCAACGAGACATCTGTGGTGATGAGGGTTGA

Protein sequence

MKLKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGDMNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAGVGHRRWLSKVVIGVGCQKWSPELDVGGGRLSLVVKCGVGVSRRRFHRSWSLEVVTEVEQRDICGDEG
Homology
BLAST of HG10018893 vs. NCBI nr
Match: XP_042974660.1 (uncharacterized protein LOC122306292 [Carya illinoinensis])

HSP 1 Score: 67.0 bits (162), Expect = 1.8e-07
Identity = 34/103 (33.01%), Postives = 61/103 (59.22%), Query Frame = 0

Query: 4   KLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGDM 63
           KLN+D +     S+ G+G +++NSKGEV++  A+   S    V+ EA+ ++ GL++  D+
Sbjct: 51  KLNVDGAFSSNGSVSGIGVIVRNSKGEVIMSAAKKELSVMTAVEVEALAIIRGLQLCIDL 110

Query: 64  NIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAGVG 107
            IQN+ +ES+S ++VK  +      +  G ++ E +E V   G
Sbjct: 111 EIQNLVIESDSLLVVKEFNKQGSSKATFGNVIREAKELVTRFG 153

BLAST of HG10018893 vs. NCBI nr
Match: XP_042965938.1 (uncharacterized protein LOC122299618 [Carya illinoinensis])

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-06
Identity = 34/108 (31.48%), Postives = 60/108 (55.56%), Query Frame = 0

Query: 3   LKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGD 62
           +K N DA+       VG+G VI++ +GEVLV   E +    +   AE+  L   L++  D
Sbjct: 866 VKANWDAAVDKKEGKVGIGVVIRDEEGEVLVATGEQMNYMTDAAIAESFALRKALEVCRD 925

Query: 63  MNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAGVGHRRW 111
           +N  NVW E ++Q +V A++  +   SR G ++E+++  +   G ++W
Sbjct: 926 LNFNNVWFEGDAQNIVNAVNTETEDMSRYGSVIEDVKNLLK--GRKKW 971

BLAST of HG10018893 vs. NCBI nr
Match: PRQ49904.1 (putative ribonuclease H-like domain-containing protein [Rosa chinensis])

HSP 1 Score: 62.0 bits (149), Expect = 5.8e-06
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = 0

Query: 3   LKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGD 62
           +KLN+D +R + S  +G G VI++  G+ L  G        ++++AEA  LL GLK+  D
Sbjct: 28  MKLNVDGTRSNQSGFIGAGGVIRDHNGDWL-SGFSINLGVGQIINAEARGLLSGLKLASD 87

Query: 63  MNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAG 105
           +NI+ + VES+S I VK + D   I   +G ++   R  + G
Sbjct: 88  LNIRKLEVESDSAIFVKLLIDGCPISHPLGNIINSCRHLING 128

BLAST of HG10018893 vs. NCBI nr
Match: XP_019190496.1 (PREDICTED: uncharacterized protein LOC109184955 [Ipomoea nil])

HSP 1 Score: 61.2 bits (147), Expect = 9.8e-06
Identity = 37/113 (32.74%), Postives = 64/113 (56.64%), Query Frame = 0

Query: 3   LKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGD 62
           LKLN DA+  + SS +GLG+V++N +G  L      + S   V +AEA+++   L  +  
Sbjct: 31  LKLNTDAALQNSSSSMGLGWVLRNDEGRFLACKNMCMHSNYTVKEAEALSVREALSWLLG 90

Query: 63  MNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAGVG-----HRRW 111
           + + NV VE +SQI+   +H  S   S  G+++++++E  A +G     + RW
Sbjct: 91  IGMGNVEVEMDSQIVFNVLHS-SSFSSSFGLLVDDVKELAATIGGVEFFYARW 142

BLAST of HG10018893 vs. NCBI nr
Match: CAE5963715.1 (unnamed protein product [Arabidopsis arenosa])

HSP 1 Score: 60.8 bits (146), Expect = 1.3e-05
Identity = 35/101 (34.65%), Postives = 56/101 (55.45%), Query Frame = 0

Query: 3   LKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGD 62
           LK N D+      S    G+VI+NS G+VL+ G   L S+   + AEA+  L  L +   
Sbjct: 392 LKCNFDSGYKQGQSFTNTGWVIRNSDGKVLLSGCAKLSSSTSALQAEALGFLHVLHVTWS 451

Query: 63  MNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVA 104
             ++ +W ES+S+ L   I +    HS+IG +L++IR ++A
Sbjct: 452 QGMRQIWFESDSKELTSII-NKCEDHSQIGTLLQDIRYWMA 491

BLAST of HG10018893 vs. ExPASy TrEMBL
Match: A0A2P6RTZ7 (Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr2g0127141 PE=4 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.8e-06
Identity = 36/102 (35.29%), Postives = 58/102 (56.86%), Query Frame = 0

Query: 3   LKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGD 62
           +KLN+D +R + S  +G G VI++  G+ L  G        ++++AEA  LL GLK+  D
Sbjct: 28  MKLNVDGTRSNQSGFIGAGGVIRDHNGDWL-SGFSINLGVGQIINAEARGLLSGLKLASD 87

Query: 63  MNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAG 105
           +NI+ + VES+S I VK + D   I   +G ++   R  + G
Sbjct: 88  LNIRKLEVESDSAIFVKLLIDGCPISHPLGNIINSCRHLING 128

BLAST of HG10018893 vs. ExPASy TrEMBL
Match: A0A803QGE0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.4e-05
Identity = 33/106 (31.13%), Postives = 61/106 (57.55%), Query Frame = 0

Query: 2    KLKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVG 61
            ++KLN+DA+  D SS  G+G+V++NS  E+L V   +     +   AEAM +   L  + 
Sbjct: 1302 EIKLNVDAAIFDSSSRFGIGFVVRNSVAELLSVVGLYRPGKPDPAVAEAMGIREALSWLK 1361

Query: 62   DMNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAGVGH 108
            D +     VES+S I V+AIH   +++S  G+++++ +  +  + +
Sbjct: 1362 DKSFNQASVESDSLISVQAIHSSVVMYSGFGLIIQDCKSLLESLNN 1407

BLAST of HG10018893 vs. ExPASy TrEMBL
Match: A0A2I4FCK8 (uncharacterized protein LOC108997520 OS=Juglans regia OX=51240 GN=LOC108997520 PE=4 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.8e-05
Identity = 31/107 (28.97%), Postives = 61/107 (57.01%), Query Frame = 0

Query: 4   KLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGDM 63
           KLN DA+      ++G+G V++NS+GEVLVV +      C    A+   LL  +K+  ++
Sbjct: 70  KLNFDAAFDSEKRLMGIGIVVRNSRGEVLVVVSAPKSHICSAFSAKCYALLRSIKLCQEL 129

Query: 64  NIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYVAGVGHRRW 111
            +  V  E +++ +V +++ ++  HS  G+++++I+  +   GH  W
Sbjct: 130 CLYQVVFEGDAKAIVDSVNGYNNSHSWQGLLIDDIQFLIK--GHADW 174

BLAST of HG10018893 vs. ExPASy TrEMBL
Match: M5X848 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G063400 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 2.4e-05
Identity = 32/98 (32.65%), Postives = 54/98 (55.10%), Query Frame = 0

Query: 2   KLKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVG 61
           K KLN+DA+ +  + + G+G VI+N KGEV+V  A  L +      AE M L  GL    
Sbjct: 236 KFKLNVDAAYIPDTGVGGIGAVIRNEKGEVMVATALPLHTATSPKHAEIMALQFGLNFAW 295

Query: 62  DMNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIR 100
           D    ++ VES+SQ +V  ++      +  G ++++++
Sbjct: 296 DAGFSSILVESDSQTVVNNLNTDEESFAPKGHLIDDVK 333

BLAST of HG10018893 vs. ExPASy TrEMBL
Match: A0A5E4ET70 (PREDICTED: reverse mRNAase OS=Prunus dulcis OX=3755 GN=ALMOND_2B008911 PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 3.1e-05
Identity = 33/98 (33.67%), Postives = 53/98 (54.08%), Query Frame = 0

Query: 2   KLKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVG 61
           K KLN+DA+ +    + G+G VI+N KGEV+V  A  L +      AE M L  GL    
Sbjct: 38  KFKLNVDAAYIPDMGVGGIGAVIRNEKGEVMVATALPLHTATSPKHAEIMALQFGLNFAW 97

Query: 62  DMNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIR 100
           D    ++ VES+SQ +V  ++      +  G ++++I+
Sbjct: 98  DAGFSSILVESDSQAVVNNLNTDEESFAPKGHLIDDIK 135

BLAST of HG10018893 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 46.2 bits (108), Expect = 3.0e-05
Identity = 27/101 (26.73%), Postives = 54/101 (53.47%), Query Frame = 0

Query: 2   KLKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVG 61
           KLK N DAS  + +++ GLG++++NS+G V+  G    +      +AE  TL+  ++   
Sbjct: 112 KLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLIWAIQASY 171

Query: 62  DMNIQNVWVESNSQILVKAIHDHSMIHSRIGVMLEEIREYV 103
               + V  E ++Q + + I+  S  + R+   L+ I+ ++
Sbjct: 172 GFGHKKVIFEGDNQTITRMINTKSS-NPRLQHFLDTIQSWI 211

BLAST of HG10018893 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 7.5e-04
Identity = 24/81 (29.63%), Postives = 44/81 (54.32%), Query Frame = 0

Query: 3   LKLNIDASRVDPSSIVGLGYVIQNSKGEVLVVGAEFLESTCEVVDAEAMTLLVGLKIVGD 62
           +K N DA+    +   G+G+V++N KGEV  +GA  L     V++AE   +   +  +  
Sbjct: 428 VKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSR 487

Query: 63  MNIQNVWVESNSQILVKAIHD 84
                V  ES+SQ+L++ +++
Sbjct: 488 FQYNYVIFESDSQVLIEILNN 508

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_042974660.11.8e-0733.01uncharacterized protein LOC122306292 [Carya illinoinensis][more]
XP_042965938.11.5e-0631.48uncharacterized protein LOC122299618 [Carya illinoinensis][more]
PRQ49904.15.8e-0635.29putative ribonuclease H-like domain-containing protein [Rosa chinensis][more]
XP_019190496.19.8e-0632.74PREDICTED: uncharacterized protein LOC109184955 [Ipomoea nil][more]
CAE5963715.11.3e-0534.65unnamed protein product [Arabidopsis arenosa][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2P6RTZ72.8e-0635.29Putative ribonuclease H-like domain-containing protein OS=Rosa chinensis OX=7464... [more]
A0A803QGE01.4e-0531.13Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2I4FCK81.8e-0528.97uncharacterized protein LOC108997520 OS=Juglans regia OX=51240 GN=LOC108997520 P... [more]
M5X8482.4e-0532.65Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_3G063400 PE=4 SV=1[more]
A0A5E4ET703.1e-0533.67PREDICTED: reverse mRNAase OS=Prunus dulcis OX=3755 GN=ALMOND_2B008911 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G65005.13.0e-0526.73Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT4G29090.17.5e-0429.63Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..123
e-value: 1.0E-7
score: 34.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 6..101
e-value: 7.0E-15
score: 54.9
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 3..103
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 5..99
e-value: 6.37736E-14
score: 62.7168
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 4..85

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10018893.1HG10018893.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity