HG10018867 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10018867
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionAldolase-type TIM barrel family protein
LocationChr04: 9922508 .. 9926608 (+)
RNA-Seq ExpressionHG10018867
SyntenyHG10018867
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTTTGGTGGAATACTTGGCTTCCAGTATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCTGTCCTGAGCTTGTTGCTGCCGTCGCCAATGCCGGCGGACTTGGTCTTCTCAGAGCCCCCGATTGGGTACTTTACTGTCTATTTCTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCATTTAGCTGTGTTGGAAATGAATGTAATGTGTTGTGGAGAAGCATCTCTGATGTGCGATACGTGAGTGGAACCGGAGTTTCTCCTTTCGTAATGGATTTCAGTGATTGATTGTTCGATCATACGTGTTCGGATTTTGAAACTTTTAATTGTTGATCGGTTTGAATGATTGGCGTTTTCGGATTTTTGCAACTGGTTGTTGCATGGTTTTATAGGAATTCAAACGATTTGGTGATGATTGCTTATGAAATTATGGAGGAGTGTTGGGTTTAACTGGGGACTTAGTCGGGGGAATTGATAATGTAAATGAACAGTTGAATCTTTGATCTTGCCAATCTGTTGTGAACAGTTTATATAAGGTATGAATGTATAGAATTTTGTTAAAGTATTGATATAGTTAAACGTACCTTAACCTATGAGCTTAAGCTTTTCAGTAGATCGGTGATTTAACAGACTCATTTTCTCTTCCGTTAATGATGATTTCCATTTGTTGATATAATTAAAATTATAGTTTAGGATTGATGGGTGATTTAACAAATTTAATGCAAGCTTCTTATAGCTATTTTTTCCCTTCCTTTGTTCTTCTATTAGTCAAGAAACTAGTTATAAAATTTTAGTATCGGTTTCATAACTATTTTGTCTTTTGTTGTCTAAGAGCTAGTGTAGTGTAGTATTTAGCTTTAGTTTATACTTTCTACAACGGTTTTGAGATTTTAGCCAAAATTTGAAAAATCAATTAAATTTTTTTGAGAACTAAATGTTTTTGGTTTGTGAATCTTGCTATGATCTCAAAAACATTTATAAAATAAATGCATGTATTAAGGACTGAACAAACTTATCTTCTTAAATCGACAACATACAGTCAAATGATTATCAAACAGTCTTTTAATTATAAAGTCAAAAGGTGTATATCTTCCCATTTATCCTAACTATTTATCTACATCATTTCCTGTTCTTCACTCTATTTGAGTAGCATGGAGCTTGTACGCTTAAAATTTTTATGTGTACCAGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCGTTAACAGATAAACCATTTGGAGTAGGTGTCATTTTAGCATTTCCTCATGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAATGTTCAAAGGATCTTGTAGATCAAGTTCATTCTGCTGGAGTGAAAATTATTCCACAAGTGAGTGTTCTCTTGCAAATCACATTAACAATGCATGTCCACAAGATCCAAATCAAGTCGATTGCTTGATCTTATCATTAAACTTTTTATGCACGGTTGTGGAGGCTTAATTTTCTAGAAAAATCAGTCATCTTATTAAATCACATGCTTCTCTTGTGTTGGTCCATCACTTCAGGCTTCATTATTCTGTTCTTGGATGATATTGGTCACTCATGTGGTATGTTACAATTTTAAAATCTACTTTTGTAGGTTGGGAGTGTTGAAGAAGCGAGTAAAGCAGTTGAAGTTGGTGTTGATGCAATAATTGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGTATGGATCTTCTTCTTCCCAGAAAAATTAATTAATTCGAGATGCTATTGTATTTGTTTCTAGACTTCTTCCATTACAGTTTCTTTTTTCCATCTTCTAAAGGGGCTATGTTTTTGTACGGTTATTCTTCTTTGTAAAACGTTCTCTAAATTACTAGAAAAAGAAACGAATTAACAAGGAAAATAAGTTGACTTAGATAAGAAAGTTAGTTCATCAAACATCCCTTTCTCAATTCATTAAGGGACTGTGGTCTTAGTTCGGATACCTACATACTTACGCTGCTCCAAGTTCATCTTTTGAAGAGTATACAACATTGGTTTTGTTGAGCCAATGGTTATATTAGAAGCCTGTATTCAGTTTGAAATCAGCTTTAGACAGAACCGGATTTTTTAATATTGATCTTTATCATAAAAGTATTTAAACATTCTTCCTTTCTGACATCAGTCATGCATACATATTTTTAAGTAATTTCCTACTTTTTTCTTCTTTAAATTTCACTGGTAGTAAGAACTGCTAGTATTTTTGTTTGTTGCTTCCAGCCAGTGATATTACTTTGGTGTATGTTTTCATTAGTCATATTCTTCACCTCTCTTTACATCTGTATTTCAACTATATCGTAACTTTTCTCAAATATATAGTCTATAGAAGTTTCATTATCAGAAAAAATGAAAATAGTCTTTAGAAGTTTGACTTAGTTCTTTACAGGAAGGTTTGATTTCCATCTTACCAAGGATAGTTGATATTGTTGGGAATGAAGATATACCTGTAATTGCGGCTGGCGGTATTTCAGATTCCAGAGGTTATGTTGCTGCCCTTGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGGTTAGAAATGCTCAACTGTGATCATTCACAACTTAGAAAACTTATTTTAAAAAGCTGGTAGATCCATGCTACAAAATATTGCTCATTTAGAAACAATTTCCAGTATTAAGAATAGAATATTGCCACACGTTCGGTTCATTCAGGTTCTGTTCAGAGTCAACATATAAGTATGATAATAATGTCAGTGCAGAAATTTTATTAGTGGTCCGTGGCATATATTCTTAAACATATCTTATTTTCATTTATCCAAGGCATGAAAATATAGCCATGAGAATGGTGTGTGAGGAAGTTCTATGGAGGTTGATTGGTCATATCATAATCCATCCACTAAATAGTTCAGAGGCAGAGAACCTGTTCTTTTTGGTGTAACCCAAAGGTTGCCGGTTTTATTTATTTATTTTTGTTAGAGAGGCAAACAAACAATACCATCAAGAAATATCCAACTACTTCCTAACCTCATCAACACGAATGAGGGGGAGAATACTATCTAGAAAAACATCTTTCCAATCAAAGAACAATCTTGAGAGGAGGCAAAAGCTGTTATGGTATGGTCAGCTTTGTTGAGGAATATGACAAAAATGACAATATTAACAAGGTTCCCAACAAAAGGAATATCCTCAACGGGAACTCCAATTTTCGCAAGCGAGAGCTCCTTACACATCAGAAGATTAATCACTTCAAGTGCGCCTGATTCAACTTCAAGTGGCCTGATTCAACTTCAAGTGGAGGATTAGATGCCGTTATCCTTACTATATTGTTGTAATTGGAACAATTATATAATCTAATAACGAAACATATCTCAGAAATTTTAGGTAATATTACTATTGATCTTTTGCATTGTCATAAGTATGAGTTAAATGGAATATTGCAGATTTGTTGCTACTGAAGAAAGCCGTGCTCACCCTACATACAAGAGAAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTTGGCCGTGCAAGATGGCCCGGGGCACCACAGCGTGTTCTACAGACGCCCTTTTACGATGATTGGAAATCACTTCCTGCCAATGAAAATGAAAGCAACCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGTGAGCTCCCTCGTTATCTCTTTTCTACTTAAACAAGTGCATAAAAGGGAGTTATCTTCTTTTATTCTTCATGCTTGAAGAAATTCTGCAATTAGTTTATACGCTGGATCATTAAAAGATTAAATATTTAGGTTAAGGATATTCTGCTGTTCTGCTCTACTTTAAATCATCCTTCATTCAATGCATTCATGCTTGAAACAGGACATAGATATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGACAAAAGGTGACATCGAGAGCATGGCAATGTATGCAGGGCAAGGAGTTGGACTAATCAAGGAAATTCTACCTGCAGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATCTTATTCAAACACATTTGAGTAGTTTTGTTCATAAAGAATGA

mRNA sequence

ATGGCTTTTGGTGGAATACTTGGCTTCCAGTATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCTGTCCTGAGCTTGTTGCTGCCGTCGCCAATGCCGGCGGACTTGGTCTTCTCAGAGCCCCCGATTGGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCGTTAACAGATAAACCATTTGGAGTAGGTGTCATTTTAGCATTTCCTCATGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAATGTTCAAAGGATCTTGTAGATCAAGTTCATTCTGCTGGAGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCGAGTAAAGCAGTTGAAGTTGGTGTTGATGCAATAATTGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGATAGTTGATATTGTTGGGAATGAAGATATACCTGTAATTGCGGCTGGCGGTATTTCAGATTCCAGAGGTTATGTTGCTGCCCTTGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGATTTGTTGCTACTGAAGAAAGCCGTGCTCACCCTACATACAAGAGAAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTTGGCCGTGCAAGATGGCCCGGGGCACCACAGCGTGTTCTACAGACGCCCTTTTACGATGATTGGAAATCACTTCCTGCCAATGAAAATGAAAGCAACCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGACATAGATATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGACAAAAGGTGACATCGAGAGCATGGCAATGTATGCAGGGCAAGGAGTTGGACTAATCAAGGAAATTCTACCTGCAGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATCTTATTCAAACACATTTGAGTAGTTTTGTTCATAAAGAATGA

Coding sequence (CDS)

ATGGCTTTTGGTGGAATACTTGGCTTCCAGTATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCTGTCCTGAGCTTGTTGCTGCCGTCGCCAATGCCGGCGGACTTGGTCTTCTCAGAGCCCCCGATTGGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCGTTAACAGATAAACCATTTGGAGTAGGTGTCATTTTAGCATTTCCTCATGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCAGTTGTGCAAGTTTACTGGGGAGAATGTTCAAAGGATCTTGTAGATCAAGTTCATTCTGCTGGAGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCGAGTAAAGCAGTTGAAGTTGGTGTTGATGCAATAATTGTGCAAGGTCGGGAAGCGGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGGATAGTTGATATTGTTGGGAATGAAGATATACCTGTAATTGCGGCTGGCGGTATTTCAGATTCCAGAGGTTATGTTGCTGCCCTTGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGATTTGTTGCTACTGAAGAAAGCCGTGCTCACCCTACATACAAGAGAAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTTGGCCGTGCAAGATGGCCCGGGGCACCACAGCGTGTTCTACAGACGCCCTTTTACGATGATTGGAAATCACTTCCTGCCAATGAAAATGAAAGCAACCAGCCTGTCATTGGCCGCTCAACAATTAATGGCTTGGACATAGATATACGACGCTTTGCGGGTACCGTGCCAAATGCAACGACAAAAGGTGACATCGAGAGCATGGCAATGTATGCAGGGCAAGGAGTTGGACTAATCAAGGAAATTCTACCTGCAGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATCTTATTCAAACACATTTGAGTAGTTTTGTTCATAAAGAATGA

Protein sequence

MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE
Homology
BLAST of HG10018867 vs. NCBI nr
Match: XP_008455173.1 (PREDICTED: probable nitronate monooxygenase [Cucumis melo] >KAA0031461.1 putative nitronate monooxygenase [Cucumis melo var. makuwa] >TYK06915.1 putative nitronate monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 649.8 bits (1675), Expect = 1.3e-182
Identity = 323/336 (96.13%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+ GVDAIIVQGREAGGHVIGQEGLISILPR+V+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHPTYKRKLVE+EATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

BLAST of HG10018867 vs. NCBI nr
Match: XP_031744639.1 (uncharacterized protein LOC101221123 [Cucumis sativus])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 322/336 (95.83%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+VGVDAIIVQGREAGGHVIGQEGLISILPR+V+IVG++DIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVRRLVEGAQHLI+THLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 336

BLAST of HG10018867 vs. NCBI nr
Match: XP_022952200.1 (uncharacterized protein LOC111454946 [Cucurbita moschata] >KAG7011538.1 yrpB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 642.5 bits (1656), Expect = 2.0e-180
Identity = 319/336 (94.94%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GGILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFRYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGVGVILAFPHEQNLKAILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+VGVDAIIVQGREAGGHVIGQEGLISILPR+VDIVGNEDIP+IAAGGISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 336

BLAST of HG10018867 vs. NCBI nr
Match: XP_038888874.1 (nitronate monooxygenase isoform X1 [Benincasa hispida])

HSP 1 Score: 642.1 bits (1655), Expect = 2.6e-180
Identity = 322/336 (95.83%), Postives = 327/336 (97.32%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M F GILGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPD+VR LIRKTRA
Sbjct: 1   MDFRGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDFVRGLIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDK FGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKTFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+ GVDAIIVQGREAGGHVIGQEGLISILPR+VDIVGNEDIPVIAAGGISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLD+DIRRFAGTVPN TTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDVDIRRFAGTVPNETTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVRRLVEGAQHLIQTH SSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHFSSFVHKD 336

BLAST of HG10018867 vs. NCBI nr
Match: XP_023511402.1 (uncharacterized protein LOC111776237 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 641.0 bits (1652), Expect = 5.9e-180
Identity = 319/336 (94.94%), Postives = 328/336 (97.62%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GGILGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGVGVILAFPHEQNLKAILDEKVAV+QVYWGECSK LVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+VGVDAIIVQGREAGGHVIGQEGLISILPR+VDIVGNEDIP+IAAGGISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 336

BLAST of HG10018867 vs. ExPASy Swiss-Prot
Match: Q0S7Q1 ((3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=ipdC PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.5e-32
Identity = 94/239 (39.33%), Postives = 132/239 (55.23%), Query Frame = 0

Query: 6   ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 65
           ++G Q+ +VQ  +G  ++ P LVA  ANAGGLG+L +      + +   I KT+ LTDKP
Sbjct: 11  LVGVQHPVVQTGMG-WVAGPRLVAGTANAGGLGILASATMTYTE-LESAITKTKTLTDKP 70

Query: 66  FGVGV---ILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEE 125
           FGV +       PH  +L  ++ E V V         ++L+ ++ +AGV +IP +G+ + 
Sbjct: 71  FGVNIRADASDAPHRIDL--LIRESVRVASFALAP-KQELIAKLKAAGVVVIPSIGAAKH 130

Query: 126 ASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYV 185
           A K    G DA+IVQG E GGH  G      +LP ++D V   DIPV+AAGG  D RG  
Sbjct: 131 AKKVASWGADAVIVQGGEGGGHT-GPVATTLLLPSVLDAV---DIPVVAAGGFYDGRGLA 190

Query: 186 AALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQT 242
           AALA GA GV +GTRF+ T+ES    + K + +     D T      +  G P RVL T
Sbjct: 191 AALAYGAAGVAMGTRFLLTQESTVPDSVKHEYLARGLQDTT---VSRKVDGMPHRVLNT 237

BLAST of HG10018867 vs. ExPASy Swiss-Prot
Match: P71847 ((3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ipdC PE=1 SV=3)

HSP 1 Score: 137.1 bits (344), Expect = 3.6e-31
Identity = 92/247 (37.25%), Postives = 133/247 (53.85%), Query Frame = 0

Query: 6   ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 65
           ++G ++ +VQ  +G  ++   LV+A ANAGGLG+L A    + D +   I K +A+TDKP
Sbjct: 10  LIGIEHPVVQTGMG-WVAGARLVSATANAGGLGIL-ASATMTLDELAAAITKVKAVTDKP 69

Query: 66  FGVGVIL-AFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAS 125
           FGV +   A      ++ ++ E V V         + L+ ++  AG  +IP +G+ + A 
Sbjct: 70  FGVNIRADAADAGDRVELMIREGVRVASFALAP-KQQLIARLKEAGAVVIPSIGAAKHAR 129

Query: 126 KAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAA 185
           K    G DA+IVQG E GGH  G      +LP ++D V    IPVIAAGG  D RG  AA
Sbjct: 130 KVAAWGADAMIVQGGEGGGHT-GPVATTLLLPSVLDAVAGTGIPVIAAGGFFDGRGLAAA 189

Query: 186 LALGAHGVCLGTRFVATEESRAHPTYKRKLVE--LEATDYTNIFGRARWPGAPQRVLQTP 245
           L  GA GV +GTRF+ T +S      KR+ ++  L+ T  T      R  G P RVL+T 
Sbjct: 190 LCYGAAGVAMGTRFLLTSDSTVPDAVKRRYLQAGLDGTVVT-----TRVDGMPHRVLRTE 247

Query: 246 FYDDWKS 250
             +  +S
Sbjct: 250 LVEKLES 247

BLAST of HG10018867 vs. ExPASy Swiss-Prot
Match: Q9HWH9 (Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=nmoA PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 4.4e-29
Identity = 114/363 (31.40%), Postives = 162/363 (44.63%), Query Frame = 0

Query: 3   FGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALT 62
           F  +LG Q  I+QAP+   +S P L AAV+NAGGLG + A    + +  R LIR+ R LT
Sbjct: 5   FTRLLGIQQPIIQAPM-LGVSTPALAAAVSNAGGLGSI-AITGSAAEKGRALIREVRGLT 64

Query: 63  DKPFGVGVILAFPHEQN---------------------------------------LKAI 122
           DKPF V +    P + +                                       L  +
Sbjct: 65  DKPFNVNLFCHRPGQADPARERAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPML 124

Query: 123 LDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGG 182
           L+E+ A V  ++G   +D V  + + G++++    + EEA+     G DA++ QG EAGG
Sbjct: 125 LEERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGG 184

Query: 183 H--VIGQE---GLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGTRF 242
           H  V   E     I  L  +  +     +PV+AAGGI D RG  AAL LGA  V +GT F
Sbjct: 185 HRGVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAF 244

Query: 243 VATEESRAHPTYKRKLVELEA--TDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENE 302
           V   ES A+  Y+  L    A  T  T         G P R+    F+D   + P     
Sbjct: 245 VLCPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRM----FFD--AAAPGVPPL 304

Query: 303 SNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRR 320
            + P +  +T                 A  +G+ +  A +AGQG  L +E LPA E++R 
Sbjct: 305 PDYPFVYDAT-----------KALQTAALARGNHDFAAQWAGQGAALARE-LPAAELLRT 347

BLAST of HG10018867 vs. ExPASy Swiss-Prot
Match: B2TEK6 (Nitronate monooxygenase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) OX=398527 GN=Bphyt_4144 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.8e-29
Identity = 115/358 (32.12%), Postives = 163/358 (45.53%), Query Frame = 0

Query: 6   ILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 65
           +LG    I+QAP+   +S P L AAV NAGGLG L      + D  R++IR+TRALTDKP
Sbjct: 13  LLGIDKPIIQAPMA-GVSTPALAAAVCNAGGLGSLGVGAMNA-DGARKVIRETRALTDKP 72

Query: 66  FGVGVILAFPHEQN---------------------------------------LKAILDE 125
           F + V    P + +                                       L   L+E
Sbjct: 73  FNINVFCHRPAQADAAVEQQWLSWLAPHFEKYGATPPAKLSDIYTSFLADPAMLAVFLEE 132

Query: 126 KVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGH-- 185
           K A+V  ++G  S D++ ++  AG++++    +++EA++    GVDAI+ QG EAGGH  
Sbjct: 133 KPAIVSFHFGLPSADVIAELKKAGIRLLASATNLQEAAQVEAAGVDAIVAQGIEAGGHRG 192

Query: 186 VIGQEGLISILP--RIVDIVGNE-DIPVIAAGGISDSRGYVAALALGAHGVCLGTRFVAT 245
           V   +     L    +  ++  E  +PVIAAGGI D  G  AALALGA    LGT FVA 
Sbjct: 193 VFDPDAFDDRLGTFALTRLLAKECRLPVIAAGGIMDGAGIAAALALGAQAAQLGTAFVAC 252

Query: 246 EESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPV 305
            E+     Y+R L+   A        R  +  A    L     + + +L A +  S +P 
Sbjct: 253 TETSIDEGYRRALLGEAAR-------RTTFTAAISGRLARSMANSFTALGA-DPRSPEPA 312

Query: 306 IGRSTINGLDIDIRRFAGTVPNAT--TKGDIESMAMYAGQGVGLIKEILPAGEVVRRL 318
                       I   AG   NA    KG+    A +AGQ   L +  LPA E+V +L
Sbjct: 313 ---------TYPIAYDAGKALNAAAKAKGEFGYGAHWAGQAAALARS-LPAAELVAQL 350

BLAST of HG10018867 vs. ExPASy Swiss-Prot
Match: O05413 (Probable nitronate monooxygenase OS=Bacillus subtilis (strain 168) OX=224308 GN=yrpB PE=3 SV=1)

HSP 1 Score: 123.6 bits (309), Expect = 4.1e-27
Identity = 99/351 (28.21%), Postives = 164/351 (46.72%), Query Frame = 0

Query: 13  IVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKPFGVGVIL 72
           I+QAP+   I+ P L +AV+N G LG L A  + +PD + + I++   LTD PF + V +
Sbjct: 14  IIQAPMAGGITKPRLASAVSNQGALGSL-ASGYLTPDLLEQQIKEIFELTDAPFQINVFV 73

Query: 73  AFPHE----------------------------------QNLKAILDEKVAVVQVYWGEC 132
               E                                  Q +  IL  KV      +   
Sbjct: 74  PLGLEMPPKDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKYKVKACSFTFDLP 133

Query: 133 SKDLVDQVHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGH------VIGQE--G 192
            +D V ++ +AG  +I    +VEEA    E G+D +++QG EAGGH        G+   G
Sbjct: 134 PEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRGAFLPSKGESAVG 193

Query: 193 LISILPRIVDIVGNEDIPVIAAGGISDSRGYVAALALGAHGVCLGTRFVATEESRAHPTY 252
           L++++P+  D +    +PVIAAGG+ D RG  AAL LGA GV +G+ F+   ES AHP +
Sbjct: 194 LMALIPQAADAL---SVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLICHESNAHPVH 253

Query: 253 KRKLVELEATD--YTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTIN 312
           K+K++E    D   T +F      G   + ++     + ++LP                N
Sbjct: 254 KQKILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQ------------N 313

Query: 313 GLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVE 320
            L   +R+       A+ + + + M+++AGQG+  + E +   +++ +L +
Sbjct: 314 TLTKAMRQ------KASLQNNHDQMSLWAGQGIRSLTEEISVKQLLNQLCQ 342

BLAST of HG10018867 vs. ExPASy TrEMBL
Match: A0A5A7SPI9 (Putative nitronate monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002230 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 323/336 (96.13%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+ GVDAIIVQGREAGGHVIGQEGLISILPR+V+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHPTYKRKLVE+EATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

BLAST of HG10018867 vs. ExPASy TrEMBL
Match: A0A1S3C1I8 (probable nitronate monooxygenase OS=Cucumis melo OX=3656 GN=LOC103495406 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 323/336 (96.13%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M+FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+ GVDAIIVQGREAGGHVIGQEGLISILPR+V+IVGNEDIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHPTYKRKLVE+EATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

BLAST of HG10018867 vs. ExPASy TrEMBL
Match: A0A0A0K422 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G064010 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.2e-182
Identity = 322/336 (95.83%), Postives = 331/336 (98.51%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M FGG+LGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 55  MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 114

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 115 LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 174

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+VGVDAIIVQGREAGGHVIGQEGLISILPR+V+IVG++DIPVIAAG ISDSRG
Sbjct: 175 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 234

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ
Sbjct: 235 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 294

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 295 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 354

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVRRLVEGAQHLI+THLSSFVHK+
Sbjct: 355 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 390

BLAST of HG10018867 vs. ExPASy TrEMBL
Match: A0A6J1GL38 (uncharacterized protein LOC111454946 OS=Cucurbita moschata OX=3662 GN=LOC111454946 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 9.8e-181
Identity = 319/336 (94.94%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GGILGF+YGIVQAPLGPDIS PELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFRYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGVGVILAFPHEQNLKAILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGSV 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+VGVDAIIVQGREAGGHVIGQEGLISILPR+VDIVGNEDIP+IAAGGISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKE 337
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHK+
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 336

BLAST of HG10018867 vs. ExPASy TrEMBL
Match: A0A6J1I3E9 (uncharacterized protein LOC111470639 OS=Cucurbita maxima OX=3661 GN=LOC111470639 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 9.2e-179
Identity = 316/335 (94.33%), Postives = 326/335 (97.31%), Query Frame = 0

Query: 1   MAFGGILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GGILGFQYGIVQAPLGPDIS PELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGVGVILAFPHEQNLKAILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVG +
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGCI 120

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           EEASKAV+VGVDAIIVQGREAGGHVIGQEGLISILPR+VDIVGNEDIP+IAAGGISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHP YKRKLVEL+ATDYT+IFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTDIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRS INGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPADENESNQPVIGRSRINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHK 336
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHK
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHK 335

BLAST of HG10018867 vs. TAIR 10
Match: AT5G64250.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 528.5 bits (1360), Expect = 4.0e-150
Identity = 252/321 (78.50%), Postives = 291/321 (90.65%), Query Frame = 0

Query: 5   GILGFQYGIVQAPLGPDISCPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDK 64
           GILGF+YGIVQAPLGPDIS PELVAAVANAGG+GLLR PDWE PDY+RELIRKT+ LT+K
Sbjct: 3   GILGFEYGIVQAPLGPDISGPELVAAVANAGGIGLLRCPDWECPDYLRELIRKTKTLTEK 62

Query: 65  PFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAS 124
           PFG+GV+LAFPH+ N+K IL+EKVAV+Q+YWG+CS++LVD  H AGVK+IPQVG+VEEA 
Sbjct: 63  PFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDDAHFAGVKVIPQVGNVEEAR 122

Query: 125 KAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRGYVAA 184
           KAV VGVDAIIVQGREAGGHVIG++GL S+LPR+VD+VG  DIPVIAAGGI D RGYVAA
Sbjct: 123 KAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGERDIPVIAAGGIVDGRGYVAA 182

Query: 185 LALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFY 244
           L+LGA GVCLGTRFVAT ES AHP YKRKL+E E T+YT++FGRARWPGAPQRVL+TPF+
Sbjct: 183 LSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDVFGRARWPGAPQRVLETPFF 242

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           DDW+SLPA+ENE NQP+IGRSTI+G++ +IRRF+GTVPN TT GD+ESMAMYAGQ VGLI
Sbjct: 243 DDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMTTTGDLESMAMYAGQSVGLI 302

Query: 305 KEILPAGEVVRRLVEGAQHLI 326
           KEILPAGEVV+ LVE AQ LI
Sbjct: 303 KEILPAGEVVKSLVEEAQALI 323

BLAST of HG10018867 vs. TAIR 10
Match: AT5G64250.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 451.8 bits (1161), Expect = 4.7e-127
Identity = 215/280 (76.79%), Postives = 252/280 (90.00%), Query Frame = 0

Query: 46  ESPDYVRELIRKTRALTDKPFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQ 105
           E PDY+RELIRKT+ LT+KPFG+GV+LAFPH+ N+K IL+EKVAV+Q+YWG+CS++LVD 
Sbjct: 4   ECPDYLRELIRKTKTLTEKPFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDD 63

Query: 106 VHSAGVKIIPQVGSVEEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNE 165
            H AGVK+IPQVG+VEEA KAV VGVDAIIVQGREAGGHVIG++GL S+LPR+VD+VG  
Sbjct: 64  AHFAGVKVIPQVGNVEEARKAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGER 123

Query: 166 DIPVIAAGGISDSRGYVAALALGAHGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNI 225
           DIPVIAAGGI D RGYVAAL+LGA GVCLGTRFVAT ES AHP YKRKL+E E T+YT++
Sbjct: 124 DIPVIAAGGIVDGRGYVAALSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDV 183

Query: 226 FGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNAT 285
           FGRARWPGAPQRVL+TPF+DDW+SLPA+ENE NQP+IGRSTI+G++ +IRRF+GTVPN T
Sbjct: 184 FGRARWPGAPQRVLETPFFDDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMT 243

Query: 286 TKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLI 326
           T GD+ESMAMYAGQ VGLIKEILPAGEVV+ LVE AQ LI
Sbjct: 244 TTGDLESMAMYAGQSVGLIKEILPAGEVVKSLVEEAQALI 283

BLAST of HG10018867 vs. TAIR 10
Match: AT3G14150.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 42.7 bits (99), Expect = 6.6e-04
Identity = 30/75 (40.00%), Postives = 39/75 (52.00%), Query Frame = 0

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           E+A KAVE GVD IIV     G  +      I++L  +V +V    IPV+  GG+     
Sbjct: 234 EDALKAVEAGVDGIIV-SNHGGRQLDYSPATITVLEEVVQVVRGR-IPVLLDGGVRRGTD 293

Query: 181 YVAALALGAHGVCLG 196
              ALALGA  V +G
Sbjct: 294 VFKALALGAQAVLIG 306

BLAST of HG10018867 vs. TAIR 10
Match: AT3G14150.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 42.7 bits (99), Expect = 6.6e-04
Identity = 30/75 (40.00%), Postives = 39/75 (52.00%), Query Frame = 0

Query: 121 EEASKAVEVGVDAIIVQGREAGGHVIGQEGLISILPRIVDIVGNEDIPVIAAGGISDSRG 180
           E+A KAVE GVD IIV     G  +      I++L  +V +V    IPV+  GG+     
Sbjct: 234 EDALKAVEAGVDGIIV-SNHGGRQLDYSPATITVLEEVVQVVRGR-IPVLLDGGVRRGTD 293

Query: 181 YVAALALGAHGVCLG 196
              ALALGA  V +G
Sbjct: 294 VFKALALGAQAVLIG 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008455173.11.3e-18296.13PREDICTED: probable nitronate monooxygenase [Cucumis melo] >KAA0031461.1 putativ... [more]
XP_031744639.11.1e-18195.83uncharacterized protein LOC101221123 [Cucumis sativus][more]
XP_022952200.12.0e-18094.94uncharacterized protein LOC111454946 [Cucurbita moschata] >KAG7011538.1 yrpB [Cu... [more]
XP_038888874.12.6e-18095.83nitronate monooxygenase isoform X1 [Benincasa hispida][more]
XP_023511402.15.9e-18094.94uncharacterized protein LOC111776237 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q0S7Q11.5e-3239.33(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA deh... [more]
P718473.6e-3137.25(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA deh... [more]
Q9HWH94.4e-2931.40Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644... [more]
B2TEK65.8e-2932.12Nitronate monooxygenase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG... [more]
O054134.1e-2728.21Probable nitronate monooxygenase OS=Bacillus subtilis (strain 168) OX=224308 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7SPI96.1e-18396.13Putative nitronate monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C1I86.1e-18396.13probable nitronate monooxygenase OS=Cucumis melo OX=3656 GN=LOC103495406 PE=4 SV... [more]
A0A0A0K4225.2e-18295.83Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G064010 PE=4 SV=1[more]
A0A6J1GL389.8e-18194.94uncharacterized protein LOC111454946 OS=Cucurbita moschata OX=3662 GN=LOC1114549... [more]
A0A6J1I3E99.2e-17994.33uncharacterized protein LOC111470639 OS=Cucurbita maxima OX=3661 GN=LOC111470639... [more]
Match NameE-valueIdentityDescription
AT5G64250.24.0e-15078.50Aldolase-type TIM barrel family protein [more]
AT5G64250.14.7e-12776.79Aldolase-type TIM barrel family protein [more]
AT3G14150.16.6e-0440.00Aldolase-type TIM barrel family protein [more]
AT3G14150.26.6e-0440.00Aldolase-type TIM barrel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004136Nitronate monooxygenasePFAMPF03060NMOcoord: 75..319
e-value: 3.3E-46
score: 158.2
IPR004136Nitronate monooxygenaseCDDcd04730NPD_likecoord: 10..234
e-value: 9.69921E-80
score: 241.233
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 3..335
e-value: 4.7E-99
score: 333.6
NoneNo IPR availablePANTHERPTHR323322-NITROPROPANE DIOXYGENASEcoord: 3..335
NoneNo IPR availablePANTHERPTHR32332:SF35SUBFAMILY NOT NAMEDcoord: 3..335
NoneNo IPR availableSUPERFAMILY51412Inosine monophosphate dehydrogenase (IMPDH)coord: 3..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10018867.1HG10018867.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0018580 nitronate monooxygenase activity
molecular_function GO:0003824 catalytic activity