HG10018653 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10018653
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RNA pseudouridine synthase 4, mitochondrial
Location	Chr04: 6355151 .. 6358618 (-)
RNA-Seq Expression	HG10018653
Synteny	HG10018653

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTACTTCCGGCACTCCTCCGTCGTTCTGTCATCCTCCGTACTTCTCTGTCTTCCACCGCTCCCGGCGGCGAAAGCCTCCATTTCTCAATTTTTCATGAGCTGAAGTCCACCCTAGCCTTGAGTAACACCACCGAAACCGTATCCCAACAGAAGAATGAGAAGGGAAGCAATAGATGGTTCACGTTACCTCCATACACTCCGACTGTAAATGGCTCGGTTTTAGGTAATCGCATACTGAACAGAGGAGCTCAAGCCGAAGAAGAAACTTCCATCACTGCAATCACTGCTCTGAAATGGGTGATTCGCTGCTGCCCGGAGCTGCCGAGGAGTCTCGTGCAAAAGCTTTTTCGACTGAGAAAGGTTCCGTCCTCAATTTCACTTAATACTATACTAAATTCTCGCAATTGAATGATCAGGTTAGCTAGCTGAAAGACTGACTTAAGATTCTTCCAATTGATTGTTAATGTGGTAGTTTGCATCGTCAGATTTCGAGTTTATTGTTAAATTTAAGCATCTTCCTCAGTTTATGAATCTTTTCCTTCTGATATATTTCAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATGTAATTTTGAAGCTCGAGACCAACTTAAAAGAGTGAGCCGTTATTTTCCATACCTAGATAGATATTAATTGCTCTATTTATATTCTGATTAAGAGAACATTTGATTAGCATGCTTTCCACATGGAAGAAAACTTCCTTAGATCTTTACTAACATGTTTAATTTATTGCAAGGTTGCAGCCAAGGACATTCTGAACATAGGCGATCAAATTTTTCTCCCCATCTCCATTCAAGTATTTCCCACAGAGAAAGCTCGGGATTATTGCAATAAAGAAGAAGAGAAATTCATACATAGTCTAGTATTATATAAGGTTTTCACTTTTCACTGTTCTTTCCTCTCTGTTGTTGCCTGAGAGGTGGGGTTTAAGAACTCTCAAGGGTCTGGGGTGCTGAATTGATTATTATAGTTGGTGGATATTATAGTTAGTAGTTATAATAGTCTATTTGGAGTGCAAATTATTTTAGTCTGAAATATTATAACTTGTATTTGGGGTGTAGACTATTTTAGTCTGGGTAGGAAATACTAAACACGGTAACAAAGAGACTTTAACAAAAAGGAAAAGAGAGGATTAGTAGGAAATAGTAAACATTATAACAAAGAAGAGTTTGAAATAGTTTTACTGTAATTAATTGTAGGTTAACTATAATAGTTGGAAGCACCAAGTATTATAATTGGAGCCCCAAACATCACTCCACTCACCTGAAATTGGGGGGTCCAAACATCTCCTTAGTTCCTTCTGCTCCTTGTGACACTTTATTTTATTGTGTCAGGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCCGTACAGGTATGGTATTCAACTTGTACCATTGATTGATCCTTTTGAATAATCATGGTCTTAGTTTACAAAAGTCTAGCAAATAAAAATGTTTATGCAGGGTGGTATTGGTGTCAAAAGAAGTTTAGATGAATTGGCTGCAAGTTGTTTAAGTTATGACTACTCAGAACCACCTCGGCTAGTAAGTTTTTCTTTAAATAATAAACTTTGACTTTGTGGGTTTGTTTAACTTCATTAATTAGCATGCCATATAATGATTTAGAAGGCATTTATATTGTCTTTCTAGTTTGATCTTTTCCCTTTTATGATGGGCATGTATTGAAATCTTTATCTAGTTACACACAAAGGTATGGGCTTTGCTTATTGCTAGGTACACAGGCTTGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGACTTCCACAAGTACTTCAGTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGAGGCATCAAAACATGTAGTTTTCTCTGTACCTGTATTGTTCCTTAAGCAAAATAAAAATCTGATAGAGATAAAAACGGAAATAAAGTACTGCTATCCTTCAAGCTGACATGATTTTACTTTCCATTGCATTGAAGGCAAACCATGCTGATAGTGAGAAAAAGATTTTGCAAAAGAGGTATTGGGCACTTGTAATTGGTTCTCCTAGACGTTCAAAGGGGTTGATTACAGCCCCACTAGGAAAGGTTTGTGTATAATTTCTTTCAAGCAACTATCTTGGAAGTTTTATCCTATATTGAATAACCATACATTTGCATTGGTTTTTGGATTATCTGTTTCTTTCCTTATATGTTGTCTTGAAATTTATGTGTGCTTCTGATGATTTTAGATTGTGTTGGATGATGGAAGATCTGAGCAAATTATGGTCATTGACAATCACAATGCCGAATCTATGCCTTTTCAGCAAGCAATTACTAGATATAAAGTGATAAAATCTCATCATGGTTTGCACTTTACCCTTTCTACATGAAGCATTTAACGTTTCACATTTGATTTTTCAAGCACGACATAATTTTAATCCAAATAGTGAAGGCACACTAGAACATCATACAAACATGCATCAAATTATATCATTTGTAGGTTTTCAATCTTTAGAGTACTAAGGCAGTTTGCATTAGGCACGTGAGATCATCTCTTGATCTTGTTTCTAATAAAGCTTGCATCTGATCATGCGACTGGAAAGCGAGTTCTTTTTTCTATCGAGAGGGTAGGATATGACTGAATTCTGAATGAGAAATTTTCAATCTAATAAAGGTTGCACACATCGTTATTCTTTCTATGTAGTTCAGAATGAGAAATTTTGAATACCGCATAACTTAGCTAGAAGACAGCTTTATGTCAAGTAGTCGGATATTACTCTACTTGCCTGACTTTTGAAAGAATGGCTCACAAAGTAAAAGAAAAAGCACTTTGCACGCCAAGAATTAACCGTTTTAAGTGAAGCATCTCCATTAAAATAAGTTCATTCATCTATAACTTGAACTGCCTATCTTATCTATGCTAAAGATAAAATATCAGGTTCTGTTCATTTATTACAACGCAATTGTGCAGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGGTACCATCCAGCACCCCATAGTTCCAACTACTACAAGTATTAATCTTATTTTCTGCAGATTTTTGGCTGAATATACCTTGCCTTGTATGTGTTTAATGCAGCTTCGTGTACACTGTGCTGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGGTGGCAAGCACATAGAAATTGGAAACCATTTCATTCTAATTTCCACAAGAATGAAGGAAAGAAACAATCCCTCCCTTTTGGTCTTAACCTTGAAAGTGGAAGTATTGCAGAAAATAACCCTCGCCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTGCGCACTGCATAATGATTCTCTAGATCATGACTGCTCGAGATTGGAATGTCTGGAGTTGGATGCTGCTTTGCCTTCATACATGCAAAGAAGTTGGGATATCATAAATAATTAG

mRNA sequence

ATGGTACTTCCGGCACTCCTCCGTCGTTCTGTCATCCTCCGTACTTCTCTGTCTTCCACCGCTCCCGGCGGCGAAAGCCTCCATTTCTCAATTTTTCATGAGCTGAAGTCCACCCTAGCCTTGAGTAACACCACCGAAACCGTATCCCAACAGAAGAATGAGAAGGGAAGCAATAGATGGTTCACGTTACCTCCATACACTCCGACTGTAAATGGCTCGGTTTTAGGTAATCGCATACTGAACAGAGGAGCTCAAGCCGAAGAAGAAACTTCCATCACTGCAATCACTGCTCTGAAATGGGTGATTCGCTGCTGCCCGGAGCTGCCGAGGAGTCTCGTGCAAAAGCTTTTTCGACTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATGTAATTTTGAAGCTCGAGACCAACTTAAAAGAGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCCGTACAGGGTGGTATTGGTGTCAAAAGAAGTTTAGATGAATTGGCTGCAAGTTGTTTAAGTTATGACTACTCAGAACCACCTCGGCTAGTACACAGGCTTGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGACTTCCACAAGTACTTCAGTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGAGGCATCAAAACATGTAGTTTTCTCTGTACCTGCAAACCATGCTGATAGTGAGAAAAAGATTTTGCAAAAGAGGTATTGGGCACTTGTAATTGGTTCTCCTAGACGTTCAAAGGGGTTGATTACAGCCCCACTAGGAAAGATTGTGTTGGATGATGGAAGATCTGAGCAAATTATGGTCATTGACAATCACAATGCCGAATCTATGCCTTTTCAGCAAGCAATTACTAGATATAAAGTGATAAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCTGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGGTGGCAAGCACATAGAAATTGGAAACCATTTCATTCTAATTTCCACAAGAATGAAGGAAAGAAACAATCCCTCCCTTTTGGTCTTAACCTTGAAAGTGGAAGTATTGCAGAAAATAACCCTCGCCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTGCGCACTGCATAATGATTCTCTAGATCATGACTGCTCGAGATTGGAATGTCTGGAGTTGGATGCTGCTTTGCCTTCATACATGCAAAGAAGTTGGGATATCATAAATAATTAG

Coding sequence (CDS)

ATGGTACTTCCGGCACTCCTCCGTCGTTCTGTCATCCTCCGTACTTCTCTGTCTTCCACCGCTCCCGGCGGCGAAAGCCTCCATTTCTCAATTTTTCATGAGCTGAAGTCCACCCTAGCCTTGAGTAACACCACCGAAACCGTATCCCAACAGAAGAATGAGAAGGGAAGCAATAGATGGTTCACGTTACCTCCATACACTCCGACTGTAAATGGCTCGGTTTTAGGTAATCGCATACTGAACAGAGGAGCTCAAGCCGAAGAAGAAACTTCCATCACTGCAATCACTGCTCTGAAATGGGTGATTCGCTGCTGCCCGGAGCTGCCGAGGAGTCTCGTGCAAAAGCTTTTTCGACTGAGAAAGGTTCGAAGAGGTTCTTCTATGATTGAAAATTCTGAATGTAATTTTGAAGCTCGAGACCAACTTAAAAGAGATCCAGCAATACTTGTTATCAATAAACCTCCTGGCTTGCCCGTACAGGGTGGTATTGGTGTCAAAAGAAGTTTAGATGAATTGGCTGCAAGTTGTTTAAGTTATGACTACTCAGAACCACCTCGGCTAGTACACAGGCTTGACAGGGACAGCAGTGGCATCTTGATAATGGGCAGGACTTCCACAAGTACTTCAGTTCTTCATTCTGTCTTCCGTGAGAAAACTTTTGAGGCATCAAAACATGTAGTTTTCTCTGTACCTGCAAACCATGCTGATAGTGAGAAAAAGATTTTGCAAAAGAGGTATTGGGCACTTGTAATTGGTTCTCCTAGACGTTCAAAGGGGTTGATTACAGCCCCACTAGGAAAGATTGTGTTGGATGATGGAAGATCTGAGCAAATTATGGTCATTGACAATCACAATGCCGAATCTATGCCTTTTCAGCAAGCAATTACTAGATATAAAGTGATAAAATCTCATCATGGATACACTTGGTTGGAGTTGTGTCCCCTCACTGGAAGAAAACATCAGCTTCGTGTACACTGTGCTGAGGTATTAGGAACGCCTATAGTTGGAGACTACAAATATGGGTGGCAAGCACATAGAAATTGGAAACCATTTCATTCTAATTTCCACAAGAATGAAGGAAAGAAACAATCCCTCCCTTTTGGTCTTAACCTTGAAAGTGGAAGTATTGCAGAAAATAACCCTCGCCTACACCTTCATTGTAAGCAACTGACTTTACCAGATGTTTCTTGCGCACTGCATAATGATTCTCTAGATCATGACTGCTCGAGATTGGAATGTCTGGAGTTGGATGCTGCTTTGCCTTCATACATGCAAAGAAGTTGGGATATCATAAATAATTAG

Protein sequence

MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRWFTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLRKVRRGSSMIENSECNFEARDQLKRDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESGSIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN

Homology

[Close]

BLAST of HG10018653 vs. NCBI nr
Match: XP_038888757.1 (RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida])

HSP 1 Score: 768.5 bits (1983), Expect = 3.2e-218
Identity = 392/478 (82.01%), Postives = 400/478 (83.68%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLPAL+RRSVILR  LSS AP GES HFSIFHELKSTLALSNTTETVS +KNEKGSNRW
Sbjct: 1   MVLPALIRRSVILRIPLSSAAPVGESFHFSIFHELKSTLALSNTTETVSHRKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPPYT TVNGS+LGNRIL RG QAE E S  AITALKWVIRCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNGSLLGNRILKRGDQAEAEISTAAITALKWVIRCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMI NSECN EARDQLKR                                    
Sbjct: 121 KVRRGSSMIGNSECNLEARDQLKRVAAKDILNIGDQIFLPISVQVFPTEKARDYCNKEEE 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTS+LHSVFREKTFEASKH      ANH DSEKKILQKRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFEASKH------ANHVDSEKKILQKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RRSKGLITAPLGK V+DDGRSEQIMVID  NAESMPFQQAITRYKVIKSHHGYTWLELCP
Sbjct: 301 RRSKGLITAPLGKFVMDDGRSEQIMVID--NAESMPFQQAITRYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           +TGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQS PFGL+ ESG
Sbjct: 361 VTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSFPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIIN 433
           SIAENNPRLHLHCKQLTLPDVS ALHNDS+D  CSRLECLELDA LP YMQRSWDIIN
Sbjct: 421 SIAENNPRLHLHCKQLTLPDVSFALHNDSIDQKCSRLECLELDAPLPLYMQRSWDIIN 470

[Close]

BLAST of HG10018653 vs. NCBI nr
Match: XP_023553811.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 750.0 bits (1935), Expect = 1.2e-212
Identity = 383/479 (79.96%), Postives = 395/479 (82.46%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLPA+LRRSVI R+  SS APGGESLHFSIF E KST ALSN TETVS+QKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPP+T TVNGS LGNRI+N GAQAE ETS TAITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSECN EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAIL INKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTSVLHS+FREKT  ASKH      ANH D EKKILQKRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKH------ANHVDREKKILQKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RR+KGLITAPL K+VLDDGRSEQIMV+D  NAESMPFQ AIT YKVIK+HHGYTWLELCP
Sbjct: 301 RRTKGLITAPLVKMVLDDGRSEQIMVVD--NAESMPFQHAITTYKVIKTHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPF  N HKN+GKKQSLPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAENNPRLHLHCK LTLPDVSCALHNDSLDHDCSRLE LELDA LPSYMQRSWDIINN
Sbjct: 421 SIAENNPRLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of HG10018653 vs. NCBI nr
Match: KAG7011636.1 (RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 747.3 bits (1928), Expect = 7.5e-212
Identity = 382/479 (79.75%), Postives = 395/479 (82.46%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MV PA+LRRSVI R+  SS APGGESLHFSIF E KST ALS+ TETVS+QKNEKGSNRW
Sbjct: 1   MVFPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSSATETVSEQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPP+T T NGS LGNRI+N GAQAE ETS TAITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSECN EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTSVLHS+FREKT  ASKH      ANH DSEKKILQKRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKH------ANHVDSEKKILQKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RR+KGLITAPL K+VLDDGRSEQIMVID  NAESMPFQ AIT YKVIKSHHGYTWLELCP
Sbjct: 301 RRTKGLITAPLVKMVLDDGRSEQIMVID--NAESMPFQHAITTYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPF  N HKN+GKKQSLPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAEN+PRLHLHCKQLTLPDVSCALHNDSLDHDCSRLE LELDA LPSYMQRSWDIINN
Sbjct: 421 SIAENSPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of HG10018653 vs. NCBI nr
Match: XP_022952780.1 (RNA pseudouridine synthase 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 746.9 bits (1927), Expect = 9.8e-212
Identity = 383/479 (79.96%), Postives = 395/479 (82.46%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLPA+LRRSVI R+  SS APGGESLHFSIF E KST ALS  TETVS+QKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPP+T T NGS LGNRI+N GAQAE ETS TAITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSECN EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTSVLHS+FREKT  ASKH      ANH DSEKKILQKRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKH------ANHVDSEKKILQKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RR+KGLITAPL K+VLDDGRSEQIMVID  NAESMPFQ AIT YKVIKSHHGYTWLELCP
Sbjct: 301 RRTKGLITAPLVKMVLDDGRSEQIMVID--NAESMPFQHAITTYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPF  N HKN+GKKQSLPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAEN+PRLHLHCKQLTLPDVSCALHNDSLDHDCSRLE LELDA LPSYMQRSWDIINN
Sbjct: 421 SIAENSPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of HG10018653 vs. NCBI nr
Match: XP_008454996.1 (PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA0031338.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa] >TYK06791.1 RNA pseudouridine synthase 4 [Cucumis melo var. makuwa])

HSP 1 Score: 745.0 bits (1922), Expect = 3.7e-211
Identity = 383/479 (79.96%), Postives = 394/479 (82.25%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLP L R SVILRTS S T P  E L+FSIFH LKSTLALSN +E VSQQKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPPYT TVN S+LGNRILNRGAQAE ETS  AITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSE N EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTS+LHSVFREKTF+ASKH      ANH DSEKKIL KRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKH------ANHVDSEKKILLKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RRSKGLITAPLGKIVLDDGRSE+IMVID  NAE MPFQQAITRYKVIKSHHGYTWLELCP
Sbjct: 301 RRSKGLITAPLGKIVLDDGRSERIMVID--NAEPMPFQQAITRYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAH+NWKPFH NFHKN+GKKQ LPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAE NPRLHLHCKQLTLPDVS ALHNDSLD DCSRLECLELDA LPSYMQ+SWDIIN+
Sbjct: 421 SIAETNPRLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]

BLAST of HG10018653 vs. ExPASy Swiss-Prot
Match: Q9LT72 (RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g19440 PE=2 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 4.0e-107
Identity = 220/475 (46.32%), Postives = 290/475 (61.05%), Query Frame = 0


Query: 13  LRTSLSSTAPGGESLHFSIFHELKSTL--ALSNTTETVSQQKNEKGSNRWFTLPPYTPTV 72
           LR  L S++P       S F      L  A   +T + S  + +    +W TLPP++PT+
Sbjct: 11  LRRQLQSSSP-----TISTFKNPTKALSAAAHQSTRSYSTTQTDDSRGKWLTLPPFSPTI 70

Query: 73  NGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLRKVRRGSSMI- 132
           +G+ +G  +L+ G   +  T  +  TAL+W++RC P+LPR+LVQKLFRLR+VRR  SM  
Sbjct: 71  DGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSV 130

Query: 133 -------------------------------------------ENSECNFEARD-----Q 192
                                                      E+ +C+ E R       
Sbjct: 131 DGDELQRSQLKRVAAKESLNVGDRIYLPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLV 190

Query: 193 LKRDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIM 252
           L +DPAI+V+NKP GL VQGG G+K S+DELAASCL +D SE PRLVHRLDRD SG+L++
Sbjct: 191 LYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVL 250

Query: 253 GRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSPRRSKGLI 312
            RT T+ +VLHS+FREKT  AS + V            K L+++Y ALVIG P R +G I
Sbjct: 251 ARTQTAATVLHSIFREKTTGASAYGV--------KKNVKSLKRKYMALVIGCPPRQRGQI 310

Query: 313 TAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSH-HGYTWLELCPLTGRKH 372
           +APL K+V+DDG+SE+I V  N N E +  Q AIT Y+VI+S  HGYTWLEL PLTGRKH
Sbjct: 311 SAPLRKVVVDDGKSERITV--NDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKH 370

Query: 373 QLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESGSIAENN 432
           QLRVHCAEVLGTPIVGDYKYGWQAH+  +PF S+   N  K+ S PFGL+L+ G ++   
Sbjct: 371 QLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS-ENNPTKQSSSPFGLDLDGGDVSSKQ 430

Query: 433 PRLHLHCKQLTLPDVSCALH--NDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           P LHLH KQ+ LP++S  L     S D D S L+ L+ DA LPS+MQ S++++ +
Sbjct: 431 PHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of HG10018653 vs. ExPASy Swiss-Prot
Match: Q5XET6 (RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At1g78910 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 4.5e-66
Identity = 129/301 (42.86%), Postives = 191/301 (63.46%), Query Frame = 0


Query: 144 RDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGR 203
           +D AI+V+NKPP LPV+G + +  S+D LAA+ LS+   E PRLVHRLDR++SG+L+MGR
Sbjct: 184 KDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGR 243

Query: 204 TSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSPRRSKGLITA 263
           T  S   LHSVF +     S    ++          + + ++YWALVIGSP+  +GLI+A
Sbjct: 244 TKESIDYLHSVFSDYKGRNSSCKAWNKAC-------EAMYQQYWALVIGSPKEKEGLISA 303

Query: 264 PLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQL 323
           PL K++LDDG+++++++      E+   Q AIT YKV+    +G +W+EL P+T RKHQL
Sbjct: 304 PLSKVLLDDGKTDRVVLAQGSGFEAS--QDAITEYKVLGPKINGCSWVELRPITSRKHQL 363

Query: 324 RVHCAEVLGTPIVGDYKYGWQAHRNWKPF-HSNFHKNEGKKQSL--PFGLNLESGSIAEN 383
           RVHCAE LGTPIVGDYKYGW  H+ WK     +     GK   L  P GL+++ GS+   
Sbjct: 364 RVHCAEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSK 423

Query: 384 NPRLHLHCKQLTLPDVSCALHNDSLDHD-------CSRLECLELDAALPSYMQRSWDIIN 434
            P LHLHC+++ LP+++  LH  +             + + L   A++PS+M+ SW++++
Sbjct: 424 VPLLHLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMS 475

[Close]

BLAST of HG10018653 vs. ExPASy Swiss-Prot
Match: Q0J4D4 (RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os08g0520100 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.9e-65
Identity = 129/311 (41.48%), Postives = 194/311 (62.38%), Query Frame = 0


Query: 133 ECNFEARDQLKRDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLD 192
           E  +  R  + +D AILV+NKPP +P++G + V  S+D LAA+ LSY   E P+LVHRLD
Sbjct: 171 EIEYLRRLVIHKDSAILVLNKPPKVPMKGNLPVHNSMDVLAAAALSYGNEEGPKLVHRLD 230

Query: 193 RDSSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIG 252
           R+SSG+L+ GRT  S + LH +F       +   V++          +   +RYWALVIG
Sbjct: 231 RESSGLLLFGRTKESFTRLHWLFTSVNLAKTNSQVWNAAC-------EAYMQRYWALVIG 290

Query: 253 SPRRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVI-KSHHGYTWLE 312
           +P+  +G+I+APL K++LDDG++E++++   H +     Q+A+T Y+V+  + HG +W+E
Sbjct: 291 TPKEREGIISAPLSKVLLDDGKAERVILA--HPSGIDGAQEAVTAYRVMGPTIHGCSWIE 350

Query: 313 LCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPF-HSNFHKNEGKKQSL--PFG 372
           L PLTGRKHQLRVHCAE LGTPIVGDYKYGW  H+ WK     +F    G+   L  P G
Sbjct: 351 LRPLTGRKHQLRVHCAEALGTPIVGDYKYGWFVHQRWKQNPQPDFEPFTGEPYKLRRPEG 410

Query: 373 LNLESGSIAENNPRLHLHCKQLTLPDVSCAL------HNDSLDHDCSRLECLELDAALPS 432
           L ++ GS+    P LHLHC+++ +P+++  L      H +       +   L   A +P+
Sbjct: 411 LEIQKGSVLSKVPLLHLHCREMVIPNIAKFLSSNGEWHENGAPWSKEKPNLLRFIAPMPA 470

Query: 433 YMQRSWDIINN 434
           +M+ SW+I+++
Sbjct: 471 HMKISWNIMSS 472

[Close]

BLAST of HG10018653 vs. ExPASy Swiss-Prot
Match: Q69K07 (RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0103500 PE=2 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 4.3e-61
Identity = 150/370 (40.54%), Postives = 201/370 (54.32%), Query Frame = 0


Query: 51  QKNEKGSNRWFTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPR 110
           +  E+G + W  LPP+ P    +    R ++RG   E        TA+KWV RCCP+LP 
Sbjct: 43  ETGERGKSPWLQLPPFAPL--DAAAAARAISRGG-GEGGDGEQGATAIKWVRRCCPDLPT 102

Query: 111 SLVQKLFRLRKVRRG-----------------------------------------SSMI 170
           SLVQKLFRLRKV++                                          SS+ 
Sbjct: 103 SLVQKLFRLRKVKKNVVTAEISSADASAEQHRLRRVSAKDQLMPGDILFLPVNLKESSVA 162

Query: 171 E-------NSECNFEARDQLKRDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYS 230
           E        +E NF    ++ +D AI+V+NKPPG+PVQGG+G+K S+D L AS    + S
Sbjct: 163 EKTKKFDNRNEINFLRGLEIYKDEAIIVVNKPPGMPVQGGVGIKNSIDVL-ASMFEENSS 222

Query: 231 EPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKIL 290
           E PRLVHRLDRD SGIL++GR   STS+LH++FREKT +A            AD  + +L
Sbjct: 223 EAPRLVHRLDRDCSGILVLGRNQLSTSMLHAIFREKTADAL-----------ADGTQHVL 282

Query: 291 QKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIK 350
           Q++Y ALVIG+PR  KGL++APL KI+L DG+SE++ +  + NA S+  Q A+T Y+VI+
Sbjct: 283 QRKYVALVIGTPRHPKGLLSAPLAKILLQDGKSERLTIRASSNAASV--QDALTEYRVIE 342

Query: 351 S-HHGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRN--WKPFHSNFHKNE 370
           S   GYTWLEL P TGRKHQ        L   ++G      +  R+    P H    +N 
Sbjct: 343 SCPQGYTWLELFPRTGRKHQ------RFLEPQLLGITSMDGKRIRSGCLFPCHGQLMRNC 389

[Close]

BLAST of HG10018653 vs. ExPASy Swiss-Prot
Match: Q28C59 (Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN=rpusd4 PE=2 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 1.2e-26
Identity = 95/314 (30.25%), Postives = 144/314 (45.86%), Query Frame = 0


Query: 110 RSLVQKLFRLRKVRRGSSMIENSECNF-------EARDQLKR-DPAILVINKPPGLPVQG 169
           R L +++   RKV  G++  E S  N        E + Q+ R DP ++++NKP GLPV G
Sbjct: 13  RMLAERIRAERKVLEGAAR-ETSRKNAPTIVYLRELKSQVVREDPELVLVNKPHGLPVHG 72

Query: 170 GIGVKRSLDELAASCLSYDY---SEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREK 229
           G  V+RS+  L  +   + +   +EP +L HRLDRD++G LI+ RT+ +   +    RE+
Sbjct: 73  GPTVERSVASLLPALAKHHFGWKAEPLKLCHRLDRDTTGALILARTTEAAERVQQALRER 132

Query: 230 TFEASKHVVFSVPANHADSEKKILQKRYWALVIGSPRRSKGLITAPLGK----------- 289
                                  + + YWAL +G+P   +G++  P+ +           
Sbjct: 133 E----------------------VHRVYWALCLGTPSPREGILDIPIMEKETSGPQKHYK 192

Query: 290 -------IVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCPLTGRKH 349
                   V ++G  E++ V            +A+TRY+ + +  G + +EL P+TG KH
Sbjct: 193 MALSPRFRVSEEGAVERVRV-------PRSAHEAVTRYRTLGAASGASLVELHPITGVKH 252

Query: 350 QLRVHCAEVLGTPIVGDYKYG-WQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESGSIAEN 394
           QLRVH A  L  PI+GD+KY  W      KP  S              GL +        
Sbjct: 253 QLRVHLALGLNCPILGDHKYSHWGRLAPQKPPDSVLR---------ALGLTVPQARTLS- 283

[Close]

BLAST of HG10018653 vs. ExPASy TrEMBL
Match: A0A6J1GMP9 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455375 PE=4 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 4.8e-212
Identity = 383/479 (79.96%), Postives = 395/479 (82.46%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLPA+LRRSVI R+  SS APGGESLHFSIF E KST ALS  TETVS+QKNEKGSNRW
Sbjct: 1   MVLPAVLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTYALSIATETVSEQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPP+T T NGS LGNRI+N GAQAE ETS TAITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATANGSALGNRIMNGGAQAEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSECN EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAIL INKPPGLPVQGGIGVKRSLD+LAASCLSYDYSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDQLAASCLSYDYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTSVLHS+FREKT  ASKH      ANH DSEKKILQKRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKH------ANHVDSEKKILQKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RR+KGLITAPL K+VLDDGRSEQIMVID  NAESMPFQ AIT YKVIKSHHGYTWLELCP
Sbjct: 301 RRTKGLITAPLVKMVLDDGRSEQIMVID--NAESMPFQHAITTYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPF  N HKN+GKKQSLPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFPHNSHKNQGKKQSLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAEN+PRLHLHCKQLTLPDVSCALHNDSLDHDCSRLE LELDA LPSYMQRSWDIINN
Sbjct: 421 SIAENSPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 471

[Close]

BLAST of HG10018653 vs. ExPASy TrEMBL
Match: A0A5D3C4F5 (RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00930 PE=4 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.8e-211
Identity = 383/479 (79.96%), Postives = 394/479 (82.25%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLP L R SVILRTS S T P  E L+FSIFH LKSTLALSN +E VSQQKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPPYT TVN S+LGNRILNRGAQAE ETS  AITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSE N EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTS+LHSVFREKTF+ASKH      ANH DSEKKIL KRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKH------ANHVDSEKKILLKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RRSKGLITAPLGKIVLDDGRSE+IMVID  NAE MPFQQAITRYKVIKSHHGYTWLELCP
Sbjct: 301 RRSKGLITAPLGKIVLDDGRSERIMVID--NAEPMPFQQAITRYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAH+NWKPFH NFHKN+GKKQ LPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAE NPRLHLHCKQLTLPDVS ALHNDSLD DCSRLECLELDA LPSYMQ+SWDIIN+
Sbjct: 421 SIAETNPRLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]

BLAST of HG10018653 vs. ExPASy TrEMBL
Match: A0A1S3BZE7 (RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495275 PE=4 SV=1)

HSP 1 Score: 745.0 bits (1922), Expect = 1.8e-211
Identity = 383/479 (79.96%), Postives = 394/479 (82.25%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLP L R SVILRTS S T P  E L+FSIFH LKSTLALSN +E VSQQKNEKGSNRW
Sbjct: 1   MVLPPLFRPSVILRTSPSYTDPSSEILYFSIFHVLKSTLALSNASEPVSQQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPPYT TVN S+LGNRILNRGAQAE ETS  AITALKWVI CCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPYTATVNASILGNRILNRGAQAEVETSTAAITALKWVIHCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSE N EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSEYNLEARDQLKRVAAKDILNVGDQIFLPISAQVFSTEKTRDYCNKDEE 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSY YSEPPRLVHRLDRD
Sbjct: 181 TFMHSLVLYKDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYGYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTS+LHSVFREKTF+ASKH      ANH DSEKKIL KRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSLLHSVFREKTFDASKH------ANHVDSEKKILLKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELCP 360
           RRSKGLITAPLGKIVLDDGRSE+IMVID  NAE MPFQQAITRYKVIKSHHGYTWLELCP
Sbjct: 301 RRSKGLITAPLGKIVLDDGRSERIMVID--NAEPMPFQQAITRYKVIKSHHGYTWLELCP 360

Query: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESG 420
           LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAH+NWKPFH NFHKN+GKKQ LPFGL+ ESG
Sbjct: 361 LTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHKNWKPFHYNFHKNQGKKQFLPFGLDFESG 420

Query: 421 SIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           SIAE NPRLHLHCKQLTLPDVS ALHNDSLD DCSRLECLELDA LPSYMQ+SWDIIN+
Sbjct: 421 SIAETNPRLHLHCKQLTLPDVSFALHNDSLDQDCSRLECLELDAPLPSYMQKSWDIINS 471

[Close]

BLAST of HG10018653 vs. ExPASy TrEMBL
Match: A0A6J1IBC5 (RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470950 PE=4 SV=1)

HSP 1 Score: 732.3 bits (1889), Expect = 1.2e-207
Identity = 378/480 (78.75%), Postives = 390/480 (81.25%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNEKGSNRW 60
           MVLPALLRRSVI R+  SS APGGESLHFSIF E KST ALSN TETVS+QKNEKGSNRW
Sbjct: 1   MVLPALLRRSVIRRSFPSSAAPGGESLHFSIFCERKSTFALSNATETVSEQKNEKGSNRW 60

Query: 61  FTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLR 120
           FTLPP+T TVNGS LGNRI+N GAQ+E ETS TAITALKWV RCCPELPRSLVQKLFRLR
Sbjct: 61  FTLPPFTATVNGSALGNRIMNGGAQSEAETSTTAITALKWVFRCCPELPRSLVQKLFRLR 120

Query: 121 KVRRGSSMIENSECNFEARDQLKR------------------------------------ 180
           KVRRGSSMIENSECN EARDQLKR                                    
Sbjct: 121 KVRRGSSMIENSECNLEARDQLKRVAANDILNIGDKLCLPISVQVFPTERAPDCCNKEEK 180

Query: 181 ----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240
                     DPAIL INKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD
Sbjct: 181 KFIHSLVLYKDPAILAINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRD 240

Query: 241 SSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSP 300
           SSGILIMGRTSTSTSVLHS+FREKT  ASKH      ANH D EK ILQKRYWALVIGSP
Sbjct: 241 SSGILIMGRTSTSTSVLHSIFREKTLGASKH------ANHVDREKMILQKRYWALVIGSP 300

Query: 301 RRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAES-MPFQQAITRYKVIKSHHGYTWLELC 360
             +KGLITAPL K+VLDDGRSEQIMV+D  NAES MPFQ AIT YKVIKSHHGYTW+ELC
Sbjct: 301 SHTKGLITAPLVKMVLDDGRSEQIMVVD--NAESIMPFQHAITTYKVIKSHHGYTWVELC 360

Query: 361 PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLES 420
           PLTGRKHQLRVHCAE LGTPIVGDYKYGWQAHRN KPF  N HKN+GKKQSLPFGL+ ES
Sbjct: 361 PLTGRKHQLRVHCAEALGTPIVGDYKYGWQAHRNLKPFPHNSHKNQGKKQSLPFGLDFES 420

Query: 421 GSIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           GSIAENNPRLHLHCK LTLPDVSCALHNDSLDHDCSRLE LELDA LPSYMQRSWDIINN
Sbjct: 421 GSIAENNPRLHLHCKHLTLPDVSCALHNDSLDHDCSRLERLELDAPLPSYMQRSWDIINN 472

[Close]

BLAST of HG10018653 vs. ExPASy TrEMBL
Match: A0A6J1D432 (RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111017405 PE=4 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 4.3e-205
Identity = 366/480 (76.25%), Postives = 398/480 (82.92%), Query Frame = 0


Query: 1   MVLPALLRRSVILRTSLSSTAPGGESLHFSIFHELKSTLALSNTTETVSQQKNE-KGSNR 60
           M+LPALLRRS+IL  + SSTA GG SLHFS+FHELKST ALSNT+ET+S++KNE KG NR
Sbjct: 1   MLLPALLRRSLILSGTPSSTARGGHSLHFSVFHELKSTFALSNTSETISEEKNEKKGFNR 60

Query: 61  WFTLPPYTPTVNGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRL 120
           WFTLPPYT TV+GSVLGNRILN+G+QAE+ETS TAITALKWV+RCCPELPRSLVQKLFRL
Sbjct: 61  WFTLPPYTATVDGSVLGNRILNQGSQAEDETSTTAITALKWVLRCCPELPRSLVQKLFRL 120

Query: 121 RKVRRGSSMIENSECNFEARDQLKR----------------------------------- 180
           RK+RRGSSMIE+SECN EA++QLKR                                   
Sbjct: 121 RKIRRGSSMIESSECNLEAQNQLKRVAAKDILNVGDQIFLPISVQVFPRERARDYCNKEE 180

Query: 181 -----------DPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDR 240
                      DPAI+VINKPPGLPVQGGIG+ RSLDELAASCLSYDYSEPPRLVHRLDR
Sbjct: 181 EKFMHSLVLYKDPAIIVINKPPGLPVQGGIGITRSLDELAASCLSYDYSEPPRLVHRLDR 240

Query: 241 DSSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGS 300
           DSSGIL+MGRTSTSTSVLHS+FREKTF ASKH        H DSEKKILQK+YWALVIGS
Sbjct: 241 DSSGILVMGRTSTSTSVLHSIFREKTFGASKH--------HVDSEKKILQKKYWALVIGS 300

Query: 301 PRRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSHHGYTWLELC 360
           PRR  GLITAPLGKIVLDDGRSE+IMVID  NAESM FQQAIT Y+VIKSHHGYTWLELC
Sbjct: 301 PRRPNGLITAPLGKIVLDDGRSEKIMVID--NAESMAFQQAITTYRVIKSHHGYTWLELC 360

Query: 361 PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLES 420
           PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNW+PF SNFHKN+GKKQSLPFGL+ E+
Sbjct: 361 PLTGRKHQLRVHCAEVLGTPIVGDYKYGWQAHRNWEPFPSNFHKNQGKKQSLPFGLDFEN 420

Query: 421 GSIAENNPRLHLHCKQLTLPDVSCALHNDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           GS+AEN+PRLHLHCKQL LP+VS AL NDSLDHD S LECLELDA LPSYM+RSWDIINN
Sbjct: 421 GSVAENSPRLHLHCKQLALPNVSGALPNDSLDHDFSELECLELDAPLPSYMRRSWDIINN 470

[Close]

BLAST of HG10018653 vs. TAIR 10
Match: AT3G19440.1 (Pseudouridine synthase family protein )

HSP 1 Score: 389.8 bits (1000), Expect = 2.8e-108
Identity = 220/475 (46.32%), Postives = 290/475 (61.05%), Query Frame = 0


Query: 13  LRTSLSSTAPGGESLHFSIFHELKSTL--ALSNTTETVSQQKNEKGSNRWFTLPPYTPTV 72
           LR  L S++P       S F      L  A   +T + S  + +    +W TLPP++PT+
Sbjct: 11  LRRQLQSSSP-----TISTFKNPTKALSAAAHQSTRSYSTTQTDDSRGKWLTLPPFSPTI 70

Query: 73  NGSVLGNRILNRGAQAEEETSITAITALKWVIRCCPELPRSLVQKLFRLRKVRRGSSMI- 132
           +G+ +G  +L+ G   +  T  +  TAL+W++RC P+LPR+LVQKLFRLR+VRR  SM  
Sbjct: 71  DGTAVGKDLLSDGDSVKSSTDNSKTTALRWILRCRPDLPRTLVQKLFRLRQVRREMSMSV 130

Query: 133 -------------------------------------------ENSECNFEARD-----Q 192
                                                      E+ +C+ E R       
Sbjct: 131 DGDELQRSQLKRVAAKESLNVGDRIYLPLSVDNDTPQTPPAKKESFQCSDEERKFVCSLV 190

Query: 193 LKRDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIM 252
           L +DPAI+V+NKP GL VQGG G+K S+DELAASCL +D SE PRLVHRLDRD SG+L++
Sbjct: 191 LYKDPAIIVLNKPHGLAVQGGSGIKTSIDELAASCLKFDKSESPRLVHRLDRDCSGLLVL 250

Query: 253 GRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSPRRSKGLI 312
            RT T+ +VLHS+FREKT  AS + V            K L+++Y ALVIG P R +G I
Sbjct: 251 ARTQTAATVLHSIFREKTTGASAYGV--------KKNVKSLKRKYMALVIGCPPRQRGQI 310

Query: 313 TAPLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVIKSH-HGYTWLELCPLTGRKH 372
           +APL K+V+DDG+SE+I V  N N E +  Q AIT Y+VI+S  HGYTWLEL PLTGRKH
Sbjct: 311 SAPLRKVVVDDGKSERITV--NDNGELVSTQHAITEYRVIESSPHGYTWLELRPLTGRKH 370

Query: 373 QLRVHCAEVLGTPIVGDYKYGWQAHRNWKPFHSNFHKNEGKKQSLPFGLNLESGSIAENN 432
           QLRVHCAEVLGTPIVGDYKYGWQAH+  +PF S+   N  K+ S PFGL+L+ G ++   
Sbjct: 371 QLRVHCAEVLGTPIVGDYKYGWQAHKAREPFVSS-ENNPTKQSSSPFGLDLDGGDVSSKQ 430

Query: 433 PRLHLHCKQLTLPDVSCALH--NDSLDHDCSRLECLELDAALPSYMQRSWDIINN 434
           P LHLH KQ+ LP++S  L     S D D S L+ L+ DA LPS+MQ S++++ +
Sbjct: 431 PHLHLHSKQIDLPNISQLLEKMQVSSDSDISDLDSLKFDAPLPSHMQLSFNLLKS 469

[Close]

BLAST of HG10018653 vs. TAIR 10
Match: AT1G78910.1 (Pseudouridine synthase family protein )

HSP 1 Score: 253.4 bits (646), Expect = 3.2e-67
Identity = 129/301 (42.86%), Postives = 191/301 (63.46%), Query Frame = 0


Query: 144 RDPAILVINKPPGLPVQGGIGVKRSLDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGR 203
           +D AI+V+NKPP LPV+G + +  S+D LAA+ LS+   E PRLVHRLDR++SG+L+MGR
Sbjct: 184 KDSAIIVLNKPPKLPVKGNVPIHNSMDALAAAALSFGNDEGPRLVHRLDRETSGLLVMGR 243

Query: 204 TSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGSPRRSKGLITA 263
           T  S   LHSVF +     S    ++          + + ++YWALVIGSP+  +GLI+A
Sbjct: 244 TKESIDYLHSVFSDYKGRNSSCKAWNKAC-------EAMYQQYWALVIGSPKEKEGLISA 303

Query: 264 PLGKIVLDDGRSEQIMVIDNHNAESMPFQQAITRYKVI-KSHHGYTWLELCPLTGRKHQL 323
           PL K++LDDG+++++++      E+   Q AIT YKV+    +G +W+EL P+T RKHQL
Sbjct: 304 PLSKVLLDDGKTDRVVLAQGSGFEAS--QDAITEYKVLGPKINGCSWVELRPITSRKHQL 363

Query: 324 RVHCAEVLGTPIVGDYKYGWQAHRNWKPF-HSNFHKNEGKKQSL--PFGLNLESGSIAEN 383
           RVHCAE LGTPIVGDYKYGW  H+ WK     +     GK   L  P GL+++ GS+   
Sbjct: 364 RVHCAEALGTPIVGDYKYGWFVHKRWKQMPQVDIEPTTGKPYKLRRPEGLDVQKGSVLSK 423

Query: 384 NPRLHLHCKQLTLPDVSCALHNDSLDHD-------CSRLECLELDAALPSYMQRSWDIIN 434
            P LHLHC+++ LP+++  LH  +             + + L   A++PS+M+ SW++++
Sbjct: 424 VPLLHLHCREMVLPNIAKFLHVMNQQETEPLHTGIIDKPDLLRFVASMPSHMKISWNLMS 475

[Close]

BLAST of HG10018653 vs. TAIR 10
Match: AT1G76050.2 (Pseudouridine synthase family protein )

HSP 1 Score: 65.5 bits (158), Expect = 1.2e-10
Identity = 50/174 (28.74%), Postives = 78/174 (44.83%), Query Frame = 0


Query: 170 DELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFS 229
           +E+  S  SY  S  P +VHRLD+ ++G+L++ +   S + L   F+  T E        
Sbjct: 212 EEMTTSPSSYAASVRPGIVHRLDKGTTGLLVVAKDEHSHAHLAEQFKLHTIE-------- 271

Query: 230 VPANHADSEKKILQKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESM 289
                         + Y +L  G P   +G I  P+G+    D  +   M          
Sbjct: 272 --------------RVYVSLTTGVPSPPQGRIEIPIGR----DSSNRIRMAAIPGGVRGG 331

Query: 290 PFQQAITRYKVIKSH--HGYTWLELCPLTGRKHQLRVHCAEVLGTPIVGDYKYG 342
             + A +RYKVI++    G   +E    TGR HQ+R H A+ +G P++GD  YG
Sbjct: 332 RARHAASRYKVIETFAGGGSALVEWRLETGRTHQIRAH-AKYMGVPLLGDEVYG 358

[Close]

BLAST of HG10018653 vs. TAIR 10
Match: AT1G56345.1 (Pseudouridine synthase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 3.0e-09
Identity = 66/266 (24.81%), Postives = 108/266 (40.60%), Query Frame = 0


Query: 109 PRSLVQKLFRLRKVRRGSSMIENSECNFEARDQLKRDPAILVINKPPGLPVQGGIGVKRS 168
           P   + K   LR+    SS  ++S  N    D L  D  ++ +NKP G+  +    V RS
Sbjct: 47  PPPPISKDIELRRAMEASS--KSSLFNLTRDDILYEDEYLMAVNKPKGVYCE---AVLRS 106

Query: 169 LDELAASCLSYDYSEPPRLVHRLDRDSSGILIMGRTSTSTSVLHSVFREKTFEASKHVV- 228
             ++       D S    L +RLDRD+SG++I+ ++    + L   F E     S   + 
Sbjct: 107 APQIV-----LDSSSEYHLANRLDRDTSGVMIITKSHKVAAKLVKAFTEHKIRKSYIALC 166

Query: 229 ---------FSVPANHADSEKKILQKRYWALVIGSPRRSKGLITAPLGKIVLDDGRSEQI 288
                     +V + H  S K    + Y AL +G           P G  V D   + ++
Sbjct: 167 IGSSPNWRRVTVSSGHGRS-KHGAWRVYAALDVGR--------VLPGGSFVRDMETTFEV 226

Query: 289 MVIDNHNAESMPFQQAITRYKVIKSHHGYTW----------LELCPLTGRKHQLRVHCAE 348
           + +++   ES   +  +    V +     +           +   P +GR HQ+R+HC +
Sbjct: 227 VSVNSVKNESCELED-VNHVIVAEGERELSCGGDDDDVVVVVRAFPRSGRTHQIRLHC-Q 286

Query: 349 VLGTPIVGDYKYGWQAHRNWKPFHSN 355
            LG PI GD KY      N + F  +
Sbjct: 287 YLGIPIRGDVKYHGVYEWNGRTFEGH 291

[Close]

BLAST of HG10018653 vs. TAIR 10
Match: AT3G52260.2 (Pseudouridine synthase family protein )

HSP 1 Score: 59.7 bits (143), Expect = 6.7e-09
Identity = 59/208 (28.37%), Postives = 95/208 (45.67%), Query Frame = 0


Query: 142 LKRDPAILVINKPPGLPV-QGGIGVKRSLDELAASCLSYD-------YSEPPRLVHRLDR 201
           L  D  ++ +NKP GL V  GG+  +R++      C   +        S  P  VHRL R
Sbjct: 100 LYEDDDLIALNKPSGLQVLPGGLFQQRTVLTQLQWCFGKNDSYIGSRESPHPVPVHRLGR 159

Query: 202 DSSGILIMGRTSTSTSVLHSVFREKTFEASKHVVFSVPANHADSEKKILQKRYWALVIGS 261
            +SGIL+  +T  + + L + F E T      +V S   +      + L K Y AL  G 
Sbjct: 160 GTSGILLCAKTKLAKTKLAAYFAEGT-----SLVGSGNLDQECGTGRKLSKIYRALADGI 219

Query: 262 PRRSKGLITAPLGKIVLDDGRSEQIMVIDNHNAESMP-FQQAITRYKVIKSHHGYTWLEL 321
               + +I  P+G +V   G ++ + V    + E  P F +     +  + +     +E+
Sbjct: 220 VEEDEVVIKQPIG-VVRYPGVAQGLYVA---SPEGKPAFSKVFVLERDREKNCSLVKVEI 279

Query: 322 CPLTGRKHQLRVHCAEVLGTPIVGDYKY 341
              +GR HQ+R+H A  +G P+VGD  Y
Sbjct: 280 --QSGRPHQIRIHLA-YMGHPLVGDPLY 295

The following BLAST results are available for this feature:

BLAST of HG10018653 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888757.1	3.2e-218	82.01	RNA pseudouridine synthase 4, mitochondrial [Benincasa hispida]	[more]
XP_023553811.1	1.2e-212	79.96	RNA pseudouridine synthase 4, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
KAG7011636.1	7.5e-212	79.75	RNA pseudouridine synthase 4, mitochondrial, partial [Cucurbita argyrosperma sub...	[more]
XP_022952780.1	9.8e-212	79.96	RNA pseudouridine synthase 4, mitochondrial [Cucurbita moschata]	[more]
XP_008454996.1	3.7e-211	79.96	PREDICTED: RNA pseudouridine synthase 4, mitochondrial [Cucumis melo] >KAA003133...	[more]

BLAST of HG10018653 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LT72	4.0e-107	46.32	RNA pseudouridine synthase 4, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q5XET6	4.5e-66	42.86	RNA pseudouridine synthase 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=A...	[more]
Q0J4D4	4.9e-65	41.48	RNA pseudouridine synthase 3, mitochondrial OS=Oryza sativa subsp. japonica OX=3...	[more]
Q69K07	4.3e-61	40.54	RNA pseudouridine synthase 4, mitochondrial OS=Oryza sativa subsp. japonica OX=3...	[more]
Q28C59	1.2e-26	30.25	Mitochondrial RNA pseudouridine synthase rpusd4 OS=Xenopus tropicalis OX=8364 GN...	[more]

BLAST of HG10018653 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMP9	4.8e-212	79.96	RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A5D3C4F5	1.8e-211	79.96	RNA pseudouridine synthase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A1S3BZE7	1.8e-211	79.96	RNA pseudouridine synthase 4, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495...	[more]
A0A6J1IBC5	1.2e-207	78.75	RNA pseudouridine synthase 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1D432	4.3e-205	76.25	RNA pseudouridine synthase 4, mitochondrial OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of HG10018653 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19440.1	2.8e-108	46.32	Pseudouridine synthase family protein	[more]
AT1G78910.1	3.2e-67	42.86	Pseudouridine synthase family protein	[more]
AT1G76050.2	1.2e-10	28.74	Pseudouridine synthase family protein	[more]
AT1G56345.1	3.0e-09	24.81	Pseudouridine synthase family protein	[more]
AT3G52260.2	6.7e-09	28.37	Pseudouridine synthase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluA-like	PFAM	PF00849	PseudoU_synth_2	coord: 148..327 e-value: 6.9E-26 score: 91.2
IPR006145	Pseudouridine synthase, RsuA/RluA-like	CDD	cd02869	PseudoU_synth_RluA_like	coord: 148..392 e-value: 5.49362E-52 score: 170.981
None	No IPR available	GENE3D	3.30.2350.10	Pseudouridine synthase	coord: 136..399 e-value: 7.8E-52 score: 178.0
None	No IPR available	PANTHER	PTHR21600	MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE	coord: 143..431
None	No IPR available	PANTHER	PTHR21600:SF53	RNA PSEUDOURIDINE SYNTHASE 3, MITOCHONDRIAL	coord: 143..431
IPR020103	Pseudouridine synthase, catalytic domain superfamily	SUPERFAMILY	55120	Pseudouridine synthase	coord: 141..395

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10018653.1	HG10018653.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding