HG10018330 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10018330
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	DNA-directed RNA polymerase II subunit 4-like
Location	Chr04: 3135049 .. 3136683 (-)
RNA-Seq Expression	HG10018330
Synteny	HG10018330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGGAGAAGAAGAAGAAAATGCTGCGGAGCTTAAAATTGGGGATGGTACCAATCTTTCTGTACATCTCTGATTTTCTATCAATATTCAATTCAATGACATTTAAGCCGCTCAATCTTTCTGTGGCAATGTGTTTGCTTCATATATTTGAAAATTTTGGATCTGTTGAGCAATTCATGTATTTAATCTTTTATTTCGCTCTTTGTGCTCTTTGTTCTAAGGTCCATAAAGAGAAAATATAAGACATGATAACTGACACTTACTTCCTTTGTCTACCTACAGAGTTCTTAAAAGCCAAGTGTTTAATGAATTGCGAAGTTTCTTTGATCCTTGAACACAAGTATGAGCAGCTTCAACAAAGGGCCGAGGATCCAACAAATCAAGTTTCTCAGTGAGATTTATGTTTATTCTTTATTGATTCATAAGTTAAATTAGAGTTTTTCTTCTTTGTTGGAATAGCTAAAATATATACTTAAATTACGATATTCATTCTCACTTGCCATACAATAACAATGGATTTTTAATTTGGTCTTCTTCTGCAGAGTGTTTGAAAAGTCATTGCAGTATGTGAAACGCTTCAGTCGCTATAAGAACCCTGATGCTGTTAGACAAGTCCGAGAGTATCCTTTAAACTTTAATGTTTTCTATTTTATGGGATGGTGGGAAGGATGAGTTGTGCATATCTACTCTATGCTATAACTAACCCCATTTTGAACTAGATTAGCTTAAAGTTCATTATACCTTTACAGCCATACTATTTTATAGGTTTCCTAGGTATCCATCCGAAAATTATGTACTACTTTGATATTTAATTCATGGAGGGAAATACAATTTACTTTATCACCTAAGTTACAAACATTAAAGTTGGTTTCATTGTTTAAAATCGCATAAAAGTGGTTAAAACTTAACTACTACCATATAGAATTCTAAGCAGATATCAACTAGCTGAGTTTGAGGTATGTATACTGGATCATTTGGTTTAATTAACTTACTTATTATTATTCTATGGGAAGGTTTATCTCCTTTTGAAATTTGCAGCTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACATCCATAGTTCCATCTTTGAAGGTATCTTTTGTCATTCCTTGTAATGCCTACATTAAAATATTTATTGGTATTTTAATTTTTATCATAAGTAGAGTAGTAACTTTTAAAAAGTTTACTGTGTGTTTTTCTAGTTTACTGTGTGTTTTTCTTTCAACAATGAATATGCATCGAGTTTTAAATTACTACTAAAAAGTTTTAAAACTAAAAATGTAATTTGAAAAAAAAAAAAGAAAGACAAAAAAATGAGTCCTTAAACTTATACGAGTGTTAAGATTGAACCCTCTAACTTACGATATTTGTAGAAATTGGACCCCTAAATGATAAAAATTGAACTCGTAAATTTAAATAGTTATAATTTCTACAATTATGTGAGTTTGATGGTCCAATTTTAACAGTTACAGCTTCGAAAAGTAAAAACAATGTTTATAGATAGAGTAGACTTGCTTATAAATACTTTTGTTGTTTTCAGACTAAAGGAAGGGTGCATGACGATGAAGCTATAGAGAAGATGTTGAATGATCTTTCATTAATAAAAAAGTTTGAGTAG

mRNA sequence

ATGTCTGGAGAAGAAGAAGAAAATGCTGCGGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCCAAGTGTTTAATGAATTGCGAAGTTTCTTTGATCCTTGAACACAAGTATGAGCAGCTTCAACAAAGGGCCGAGGATCCAACAAATCAAGTTTCTCAAGTGTTTGAAAAGTCATTGCAGTATGTGAAACGCTTCAGTCGCTATAAGAACCCTGATGCTGTTAGACAACTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACATCCATAGTTCCATCTTTGAAGACTAAAGGAAGGGTGCATGACGATGAAGCTATAGAGAAGATGTTGAATGATCTTTCATTAATAAAAAAGTTTGAGTAG

Coding sequence (CDS)

ATGTCTGGAGAAGAAGAAGAAAATGCTGCGGAGCTTAAAATTGGGGATGAGTTCTTAAAAGCCAAGTGTTTAATGAATTGCGAAGTTTCTTTGATCCTTGAACACAAGTATGAGCAGCTTCAACAAAGGGCCGAGGATCCAACAAATCAAGTTTCTCAAGTGTTTGAAAAGTCATTGCAGTATGTGAAACGCTTCAGTCGCTATAAGAACCCTGATGCTGTTAGACAACTATGTGTTCTTGGCAACCTTTGCCCTGAAACTGTGGAGGAAGCTACATCCATAGTTCCATCTTTGAAGACTAAAGGAAGGGTGCATGACGATGAAGCTATAGAGAAGATGTTGAATGATCTTTCATTAATAAAAAAGTTTGAGTAG

Protein sequence

MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVRQLCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIEKMLNDLSLIKKFE

Homology

[Close]

BLAST of HG10018330 vs. NCBI nr
Match: TYK00568.1 (DNA-directed RNA polymerase II subunit 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 238.0 bits (606), Expect = 4.2e-59
Identity = 123/134 (91.79%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ----------LCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAI 120
           YVKRFSRYKNPDAVRQ          LCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAI
Sbjct: 61  YVKRFSRYKNPDAVRQVREYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAI 120

Query: 121 EKMLNDLSLIKKFE 125
           EKMLNDLSLIKKFE
Sbjct: 121 EKMLNDLSLIKKFE 134

[Close]

BLAST of HG10018330 vs. NCBI nr
Match: XP_008459458.1 (PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >XP_008459459.1 PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >KAA0039380.1 DNA-directed RNA polymerase II subunit 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 236.5 bits (602), Expect = 1.2e-58
Identity = 123/138 (89.13%), Postives = 124/138 (89.86%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEATSIVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of HG10018330 vs. NCBI nr
Match: XP_004141497.1 (DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_031742047.1 DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >KAE8648963.1 hypothetical protein Csa_008563 [Cucumis sativus])

HSP 1 Score: 235.3 bits (599), Expect = 2.8e-58
Identity = 123/138 (89.13%), Postives = 124/138 (89.86%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEATSIVPSLKTKGRVH+
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHE 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of HG10018330 vs. NCBI nr
Match: XP_038890773.1 (DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida] >XP_038890774.1 DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida])

HSP 1 Score: 234.2 bits (596), Expect = 6.1e-58
Identity = 122/138 (88.41%), Postives = 123/138 (89.13%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEA S+VPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISVVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of HG10018330 vs. NCBI nr
Match: KAG7033258.1 (DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 234.2 bits (596), Expect = 6.1e-58
Identity = 117/124 (94.35%), Postives = 120/124 (96.77%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQLCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIEKMLNDLSLI 120
           YVKRFSRYKNPDAVRQLCVLGNLCPETVEEA ++VPSLK KGRVHDDEAIEKMLNDLSLI
Sbjct: 61  YVKRFSRYKNPDAVRQLCVLGNLCPETVEEAIAMVPSLKAKGRVHDDEAIEKMLNDLSLI 120

Query: 121 KKFE 125
           KKFE
Sbjct: 121 KKFE 124

[Close]

BLAST of HG10018330 vs. ExPASy Swiss-Prot
Match: O48890 (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 5.4e-57
Identity = 114/138 (82.61%), Postives = 120/138 (86.96%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEA ++VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of HG10018330 vs. ExPASy Swiss-Prot
Match: O15514 (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 4.6e-16
Identity = 51/137 (37.23%), Postives = 85/137 (62.04%), Query Frame = 0


Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVR--------------QLCVLGNLCPETVEEATSIVPSLKTKGRVHDD 121
             RFSR+KN + +               +L  L NLCPET EE+ +++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDLSLIKKFE 125
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

[Close]

BLAST of HG10018330 vs. ExPASy Swiss-Prot
Match: Q9D7M8 (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d PE=1 SV=2)

HSP 1 Score: 85.5 bits (210), Expect = 4.6e-16
Identity = 51/137 (37.23%), Postives = 85/137 (62.04%), Query Frame = 0


Query: 2   SGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQY 61
           +G+ EE+A++L    EF  A+ L+N EV ++LEH+ +Q  + AED   ++S+VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQ-NESAED-EQELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVR--------------QLCVLGNLCPETVEEATSIVPSLKTKGRVHDD 121
             RFSR+KN + +               +L  L NLCPET EE+ +++PSL  +GR  D+
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSL--EGRFEDE 129

Query: 122 EAIEKMLNDLSLIKKFE 125
           E ++++L+D+   + F+
Sbjct: 130 E-LQQILDDIQTKRSFQ 141

[Close]

BLAST of HG10018330 vs. ExPASy Swiss-Prot
Match: Q54S04 (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689 GN=polr2d PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.8e-12
Identity = 47/147 (31.97%), Postives = 78/147 (53.06%), Query Frame = 0


Query: 4   EEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAED-------------PTNQ 63
           +EEE+   LK   +   AK L+N EV+++LEH+    +    +               N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  VSQVFEKSLQYVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVP 123
           + + F K+L Y ++FSRYKN  +++Q              +  L NLCPE  +EA S++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

[Close]

BLAST of HG10018330 vs. ExPASy Swiss-Prot
Match: Q9VEA5 (DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-11
Identity = 42/126 (33.33%), Postives = 72/126 (57.14%), Query Frame = 0


Query: 6   EENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRF 65
           +E+AA+L+   EF  A+ L+  EV ++L+H+  + Q  + D   + S+VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHR--KRQNESADEEQEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVR--------------QLCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIE 118
            ++KN + +               +L  LGNLCPE  EEA +++PSL  +GR  D+E + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSL--EGRFEDEE-LR 130

[Close]

BLAST of HG10018330 vs. ExPASy TrEMBL
Match: A0A5D3BN95 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001830 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 2.1e-59
Identity = 123/134 (91.79%), Postives = 124/134 (92.54%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ----------LCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAI 120
           YVKRFSRYKNPDAVRQ          LCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAI
Sbjct: 61  YVKRFSRYKNPDAVRQVREYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAI 120

Query: 121 EKMLNDLSLIKKFE 125
           EKMLNDLSLIKKFE
Sbjct: 121 EKMLNDLSLIKKFE 134

[Close]

BLAST of HG10018330 vs. ExPASy TrEMBL
Match: A0A1S3CA78 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC103498588 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.0e-59
Identity = 123/138 (89.13%), Postives = 124/138 (89.86%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEATSIVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of HG10018330 vs. ExPASy TrEMBL
Match: A0A5A7T811 (DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001820 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.0e-59
Identity = 123/138 (89.13%), Postives = 124/138 (89.86%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQ+SQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQISQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEATSIVPSLKTKGRVHD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEATSIVPSLKTKGRVHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of HG10018330 vs. ExPASy TrEMBL
Match: A0A0A0KXP7 (RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G643990 PE=4 SV=1)

HSP 1 Score: 234.2 bits (596), Expect = 3.0e-58
Identity = 120/124 (96.77%), Postives = 121/124 (97.58%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQLCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIEKMLNDLSLI 120
           YVKRFSRYKNPDA   LCVLGNLCPETVEEATSIVPSLKTKGRVH+DEAIEKMLNDLSLI
Sbjct: 61  YVKRFSRYKNPDA---LCVLGNLCPETVEEATSIVPSLKTKGRVHEDEAIEKMLNDLSLI 120

Query: 121 KKFE 125
           KKFE
Sbjct: 121 KKFE 121

[Close]

BLAST of HG10018330 vs. ExPASy TrEMBL
Match: A0A6J1JVN3 (DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC111489290 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.6e-56
Identity = 119/138 (86.23%), Postives = 121/138 (87.68%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQ +EDPTNQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEA S+VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAISMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSLIKKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of HG10018330 vs. TAIR 10
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 221.5 bits (563), Expect = 3.8e-58
Identity = 114/138 (82.61%), Postives = 120/138 (86.96%), Query Frame = 0


Query: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
           MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHK+EQLQQ +EDP NQVSQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQ--------------LCVLGNLCPETVEEATSIVPSLKTKGRVHD 120
           YVKRFSRYKNPDAVRQ              LCVLGNLCPETVEEA ++VPSLKTKGR HD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLNDLSLIKKFE 125
           DEAIEKMLNDLSL+K+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of HG10018330 vs. TAIR 10
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein )

HSP 1 Score: 66.6 bits (161), Expect = 1.6e-11
Identity = 39/118 (33.05%), Postives = 66/118 (55.93%), Query Frame = 0


Query: 21  AKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQYVKRFSRYKNPDAVR----- 80
           A+C+M+CE   IL+    QL   +EDP+ ++   ++++L YV+    Y NP +VR     
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  ---------QLCVLGNLCPETVEEATSIVPSLKTKGRVHDDEAIEKMLNDLSLIKKFE 125
                    ++CV+ N   E+V+E  + +PSLKTK  V  ++ ++  L +LS +KK E
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTKKEV-INQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:

BLAST of HG10018330 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK00568.1	4.2e-59	91.79	DNA-directed RNA polymerase II subunit 4-like [Cucumis melo var. makuwa]	[more]
XP_008459458.1	1.2e-58	89.13	PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Cucumis melo] >XP_0084...	[more]
XP_004141497.1	2.8e-58	89.13	DNA-directed RNA polymerase II subunit 4 [Cucumis sativus] >XP_031742047.1 DNA-d...	[more]
XP_038890773.1	6.1e-58	88.41	DNA-directed RNA polymerase II subunit 4-like [Benincasa hispida] >XP_038890774....	[more]
KAG7033258.1	6.1e-58	94.35	DNA-directed RNA polymerase II subunit 4 [Cucurbita argyrosperma subsp. argyrosp...	[more]

BLAST of HG10018330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48890	5.4e-57	82.61	DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana OX=3702 GN=NRPB...	[more]
O15514	4.6e-16	37.23	DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens OX=9606 GN=POLR2D PE...	[more]
Q9D7M8	4.6e-16	37.23	DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus OX=10090 GN=Polr2d P...	[more]
Q54S04	1.8e-12	31.97	DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum OX=44689...	[more]
Q9VEA5	1.5e-11	33.33	DNA-directed RNA polymerase II subunit Rpb4 OS=Drosophila melanogaster OX=7227 G...	[more]

BLAST of HG10018330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BN95	2.1e-59	91.79	DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3CA78	6.0e-59	89.13	DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5A7T811	6.0e-59	89.13	DNA-directed RNA polymerase II subunit 4-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A0A0KXP7	3.0e-58	96.77	RPOL4c domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G643990 PE=4...	[more]
A0A6J1JVN3	1.6e-56	86.23	DNA-directed RNA polymerase II subunit 4-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]

BLAST of HG10018330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09920.1	3.8e-58	82.61	RNA polymerase II, Rpb4, core protein	[more]
AT4G15950.1	1.6e-11	33.05	RNA polymerase II, Rpb4, core protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006590	RNA polymerase Rpb4/RPC9, core	SMART	SM00657	rpol4neu2	coord: 16..124 e-value: 4.1E-41 score: 152.6
IPR038324	Rpb4/RPC9 superfamily	GENE3D	1.20.1250.40		coord: 2..124 e-value: 2.4E-40 score: 139.4
IPR005574	RNA polymerase subunit RPB4/RPC9	PFAM	PF03874	RNA_pol_Rpb4	coord: 77..118 e-value: 1.5E-6 score: 28.5
None	No IPR available	PANTHER	PTHR21297:SF3	DNA-DIRECTED RNA POLYMERASE II SUBUNIT 4-LIKE	coord: 1..124
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 1..124
IPR010997	HRDC-like superfamily	SUPERFAMILY	47819	HRDC-like	coord: 3..122

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10018330.1	HG10018330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0031990	mRNA export from nucleus in response to heat stress
biological_process	GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0034402	recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0000932	P-body
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0030880	RNA polymerase complex
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0031369	translation initiation factor binding