HG10018293 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10018293
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptiontRNA (guanine(26)-N(2))-dimethyltransferase
LocationChr04: 2731146 .. 2738246 (-)
RNA-Seq ExpressionHG10018293
SyntenyHG10018293
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACAGATCTTAGCGAATTCACCATCACCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAAAATGAAGTCTTTTTCAATAAGACCCAGGTGCCTTGTTTATTTGAATAATGGGTTTTCTGGGGTTTTGGAGTTTAGGCATTGTTCTTAGTTAAAATTAATTGGTCAGTCAAAAGTTTCAATGGATTCTAGAGGCGGGGAAAGGATTTACATACGATTGTTTCTAGCATTTGAAGTCAGGCGTGGTTATTGATAGGGGAAATATTTAAGACCTGACTTCTATTCTTATTGAAGTGGGAAAGCTTCTTGGGGAGAAGATGTAGCTTAGGGATATCTTCCAATTTGGTTAAGTGAAGAGGCTTTGTAATTGTTGACAGTATCGAGAAACTTCAGCATGTTAGCATATTTAAATGATTGATATTTGATGGAGAAAATAAGATCTATAATTTATTCAAGTCAACAAAGTATAATGTGGTATGCTAAATTCAAAGATATTGTTCCATGTAGCATGCACAACATGATGGCAATCTATTTAATCTTATGAATAACTTATCGTTCTTTTTAAGATTCTTAGCAGCAGCCTTAGCTAAATCATCTTCTTCTCACTGTACTGCTTTTACTACATAATCTGTTTCAAGTCCAAGCAGAGTCCCGTCAGTAATCAAGAGTATCTGGGAATACTTTTCCTTCATGAAAACTATTACATACTCCTTTTAAATCTCTAATATAATCATAGAATTATTGGTTGTTGGATTTTCATTTTTTTTTTTCATTTTTTCTTTTTTGACTCTACACTGGCTTCAAGCTTTTTCTCATTATCGAATCTTGACTTTTCACCTATGTAATACTCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGTCATTTATTACAAAACGCAAACGGGAGCATGAAGCAAAACTATCAAAAAGAGCAAAATCAGGGCAGAAGATTTCTGAAAATAATGGTTCTGAAACTGCTATGGAAGAAGCATCTAATGAAACTGCAACAAATGATGAAAAGTGCCATACAGATTGTGATGCTCATGAAAAACCATCGAATGAATGTGGAAGTCCAATGTTGTCGGAAGAACAAAAAGAGATCATAGAGGGAAAAGAACAAAGAGATCCCAAGCCAATTAGAGTTCTTGAGGTTGTTTTAATTTCATTGACATTTTATTACGTGCATGTGCAAAATTAATTCTTAGTTTATCGTCCAATAAAGTGTAAAGTGTATACATTTCAAATTAGGTATATGTTCATGACTGGATTATTAGCCTGCTGTTTGAGTAATATAGTGTCTTACATTCTATAATCTGTCTTTCCCCTACAAACACAACCAAAATTCTGAACTGATACTACTACTGGGAAGGACCTTTGTGAGACCCCCAATTGGAGATAAGTATGTTATGAGGGGCAGAGATGGAAATTACCCCCTTAGAAGTGGTTAGTTGGTTGCTGTATATATTTTGGGCTTTCTTGTTTCTTGGGGTTATGTTATGTTTTGTGTAATATACTGTGGTGAGTATATCACAGAATGGAGAGTGTCCACCCTCTCGAAGTGGTGGTTTGCTTGTGTAATTCTCTTCTGATAATCTAAGTACACCCTTGTGAGGTAGTATTCTTACAACCTTCTAGAGATATTCAAAGAAGTATGGCAACAATTTATTAATTAGCAGTGGATAGACCAAGAGGCTTGAGGTTAACCTGTCTTACATTGGGATTTTTTTTAACAAGATGACTGAGTTTGTTAGTACTTTGAATTTTCCTTTATGGCAAAAATTTTAGAACAATGCAACATAGCTGGGGGCTTTTCCAACTCCAAGTTTTTAGATGGTTGACCTTTTGTATGTATTTTGTATTGGGATATGATGAGGGCACTAAGGTTGTGTCAACCTAGTTGAGATAGATGCGCCCACTGTCCCTTTTGTTGTTTGTTGTTTGTTTGCTAAGAACTCTATGATTTCTATGTCTCATTGTAACTTGGGCAATCAACAATACTCTTTTCATTATATCAAGGAAAAGTTTTGTTTCCGTTTCAAAAAAAAAAAAATATCTATCTTCCACCAAATGTCGCTTCCAATTGTTAGCTCCCTCAAGTTGAATTAGCCACTGTGGCATAACTTTTTTTAACAAATGGAAGACCAAGGGAGAGGAAGACGTAAAAGAAAAAAAGATGTTATATAACATGATGAATTTTGAATAAAGATACTACTGCATATCTGATATTTGAATTTTTTTTCTTCTTATATGATAGTTGGGTCATTAGTTCAGAGTTTTTCTTATTTTCATTTTATATCTTTATTTTAAATCTAATTTTGGTTTTCATACATACACTTTTGCTGTGCATCACTGTATTGTTAAACACTATGTAATTAGCTTCTCAAATTTTTACTGATGCCCTATGGCTTATCATCTCGTTCATATAGTAGTTGAAATTTCAAGTACCATAATGTAAATTGCATCTTTTATGTGAAATTTATGTAGATTCGTCAAAATATACAAATATATTAGATAAAGTTGTTCTTAATAGTGTGTATATGTCATGCTCATTAATATGAATATGTCCCTGTTCTAACATATTAGAATTTTCTGTTCAAAACATGTTGTCGTCATGTTTCTTGACTCCGATGTTATATTGTGCTTCCAAAATTTTATTTGTGTTTGCTTCTCTTTTGCGATGCCTGGTATGAGTTACAGTGATATGTTGTCCATTCACTACTTTCTGAGTAATTCCTGTTTGAATTTGAAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGGGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGGTAACTTTTTTAATTATTGATTATACCAAAAACTTCTCTTCAGCAAATATATTAACTTGGCCATTCTTCAGGAGTGGGAGGGTTAACAATTTGGTTAGTAACGAAAAATACTAACTTAAATAATGAGGAATGCATTATTGACCTTACCCTGATCTAGGCCTGATATATATTTTCGTGTTTGATATTATTTTTTTAAACGCGTGTGTATATATATGGGGGAGGGATATCTTATCTTGCTCTTTTCTTAATTTTTTGTGTTTTTTTTATTTATTTTTGTGGAATAAAATTTCTATTGTCTTAAAGAAAATAAAGAAGAAGAAAAAAAAAGAATCTTGCAAGAGTCTGAAGTTTATGAGGTTCTTAGGTTGTCCATTTCCCATATCAATGGATACATCTGTACACAAATTTCATATGTACAAAACACAAAGAGATCGAAGTGATTACTCATCATGAAGCACTCTACTTCTACGAGGTATTTAATAAATGGAATCAGGAATGCCACCTTTGGTTGACACTGTAATATCAAAGGCAACTTTCCTTGTACAAGCAGAGGAAGTTAGTGTTGATAATTCTTTCCCCTTTTATCTGTATATGCTTATGTATTCATCTTCTGGAGAGCACGTATTCATGTCTCGGACTGCCAGTGGAACCTTGGAAATGGAAGTTATCCATTTTACTAATCTTACAATATGCTCTTATCTATATTGATTTGTCATTTAGCTGTTCCTTTTTAGTTATCGGTTACTAACAGAACCTGTTAATGAAAAGTTTTTCTTTTCCAACAGCATCGGTTGAAGCTTGTCGGAGAAACATCAAGTTTAATGGTTCAGTAGCGTGTGCAAAAGTGGAATCACATCTAACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTAAGCACACACTAATTCAGATATGTAGGTTGGTTGGCTCTTTTGAAATTACATATAGGGAAGTAACTATTGTAATGCATTTTCTTTTTCCTTGTTATAGTTTAGAATTTAACCACATGAATAATTATGTAAAGCATGGTGATATGAGCACTGACGATAATACACAATTGAAACTTTTATGCAACTTATTTGAATTGTTTCAGGTGGATTTGGATCCTTACGGTTCACCTTCTATTTTCTTGGACTCTGCTGTTCAATCAGTTGTAGATGGTGGCATGCTGATGTGTACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGTGAAGTTTGCTACTCAAAGTAAGGTTTTTTACAGTCCTCAATGATCATTTTTTAAAAATGTTTTATTGCCATAGATTCTGTGTCCTAAGAATTACTCTGATTGATTCAGATATGGTTCCTATCCTTTGAGGGGGAAATATTGCCATGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGGTATACCTATCTCCTGTGTATTTCAATAGTTTTCTTGATGAATTATTTATTCTTCTTCTTCGGTTTAACTTGCTGTACAAGATCATTCTTATCTTAACACACACACACTAACGAGCAGAAGTGGCCTCTTATGCGTTATTTTTTTTCTAAATTCTTTGTAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCAGTGCTATCTGTCCAGATGGACTTCTATGTCCGGGTTTTTGTTCGTGTTTATACGTAAGTTGCCTTGGACTTTGAATTTACTGGGTTAGCTATATTTTACTACTTTGTTGATGTGTATTAAATATTTATATAAAGATTGTGAAATAATTGACTGCAGTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGCAGAACCGTCTCCAAGGTATATATATTTATGCTGGTTAATCACATAATTGAGTTTGTTTTTATTAACCGGTGTAATTTGTCAAACTGATGTGAGTTTTTCCTGCTTATTTTTATACACTTCCCCCTGCCCCTTTTTATCTTTTAAACAAGTTGAACGAGAGTAAAACAAAGAGCCCACAATAATGGAACAGCCAGCCAATAGTGGAGAACACAGAAAGCAAATTACTATAATGGGCCTTAACTTTTGGTGATAAAAAATTATTTCAAAGAAGTTCTGTATTATGAGTGCAACGGCACCCATTAAAATGCACCAACCCACACACTTCTTCCTCACCTTTATCTAGGCATTTTCCCTGGGCTAGGATGGATTTCTTATCCCTTTTTTGTCCCTAATATGTAACATTTGTTTCTCCTTGAAGGAAAAAAGAAAAAATGTGGCCAATGTTACCTATTGGTCTTTAAACCAATCAATATCTACTGATGCTTCTATGCTCTTTATATTATATGTTTACTTCTGATTAGAAGTTAATCCTAGTTTGAAGTAGTAATTTCTTTAAATATGAATTTGTATAGTTTCTTGATGTGTCACCTATCCTTTTGCTTTCAGAACAACAGCGTCAGGTATTTACCTGGTTTTGGTCCTTCTGTTGCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTAGGAAGGACTCTTATCCTGCATATGATCGCATATCTGCTGTACTGACAACAATTTCAGAGGTGCGTTTTATGTGTGGGTCTGGCATTACAACTCCATATTGAACTATTAAGTATAACTTTAAGCCATTGGAATTATGCTATTAGTTATTCTTGTCAAGATGATTTTGTAATTTTAGCCATTACCTAATTTCCCTCATCAAAAGAATGAAACAAAAAGTTCGAAAATGCAGTCAGTTTGGCCAATCAGAAAAATCATTATGATAAATTTGAAACCAGAAGATAAACTCATATAGATGCATTTGGAAGATTAAGTGTAGTTTATAGGATAATTATCTTTTTCCTTTTCTTTTCTCCATAAGAGTTTTGCAAGTTACAATAATAAAGTATGTTTATTGCAATATTCTAGATAACCCCGCATAATTCATTCTGGAGTCATCTCCAATTCCCTGGCATTCCATTGGCCAGCCTCCACAAGGGATTTGCCCTGAGATCTTGTCACCTCGACCATCACATGATACCTAAAAGAAAAAGGTCCAATAATTTCATAGATTGGTTAATTTTCTTACTCGTGAAGGATTAAGTACAACATAGGTTAAGTACAATATGCAGATAGTTTACTAGCAACACCATGATTAAAGCTTGTTTCCGTTTAAAAAAAAAACACCACCATGGTTAAAAGTCATCCCAAGCATACTCTTATTGATCTGGCTGTTATGTGCTGATAGTGTTTTTCTTCATGTGTTGCATTTATTGACTTGGATGTATTTTTCCTGTGCAGGAGCTGATGGACGTTCCTTTATTTTTGAGTCTACACAACCTCTGTGCAACATTAAAGTGCACATCTCCATCAGCTGTAAATTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCATTAGGACTGAAATCGGATGCACCCATGGATGTTATTTGGGATATTATGCGTTGCTGGGTAAGGCATTTTGAACTATAAATTTCATTATATACTTGAATGATATTTGTTTTTATATTACAGCCTTGTTATCAGACGTGGAACTGCAGAGTTTCGTTAATTCTTTGTCTTATTTGTATCATGGTTTCATCGATCCTTTCTATGTAGGTCAAAAACCACCCAGTTAAAGCTCAACCAGAAGATCAGCCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGTAAGTTTCATTTTGTAGTTCAACTGTTGGTTCATGATAATAACATTAAGTTGCAATGTTTGTGTCCTCTGTTTTCCACTGTCAAACATATTATTATTTTCATGAGCTAAATTTTGAGTTACCTTCAGAAATGTACCTCAAAATGAATGGCCTGTGGCTGATGTACTTTTGCCATTCTGATCTTCTTGGCAGGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAACACATCTCTCTTCTGGGTGCAGAGGCTGTAAATGGAGCTCTTAGCCATGCGGATTCTGAAGAGCCTAATGCAAAACGTCCCAAGGTAACAGAGGAAGAACCAGCTCCCAACCAGTAA

mRNA sequence

ATGGCGACAGATCTTAGCGAATTCACCATCACCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAAAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGTCATTTATTACAAAACGCAAACGGGAGCATGAAGCAAAACTATCAAAAAGAGCAAAATCAGGGCAGAAGATTTCTGAAAATAATGGTTCTGAAACTGCTATGGAAGAAGCATCTAATGAAACTGCAACAAATGATGAAAAGTGCCATACAGATTGTGATGCTCATGAAAAACCATCGAATGAATGTGGAAGTCCAATGTTGTCGGAAGAACAAAAAGAGATCATAGAGGGAAAAGAACAAAGAGATCCCAAGCCAATTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGGGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGCATCGGTTGAAGCTTGTCGGAGAAACATCAAGTTTAATGGTTCAGTAGCGTGTGCAAAAGTGGAATCACATCTAACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTGGATTTGGATCCTTACGGTTCACCTTCTATTTTCTTGGACTCTGCTGTTCAATCAGTTGTAGATGGTGGCATGCTGATGTGTACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGTGAAGTTTGCTACTCAAAATATGGTTCCTATCCTTTGAGGGGGAAATATTGCCATGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCAGTGCTATCTGTCCAGATGGACTTCTATGTCCGGGTTTTTGTTCGTGTTTATACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGCAGAACCGTCTCCAAGAACAACAGCGTCAGGTATTTACCTGGTTTTGGTCCTTCTGTTGCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTAGGAAGGACTCTTATCCTGCATATGATCGCATATCTGCTGTACTGACAACAATTTCAGAGGAGCTGATGGACGTTCCTTTATTTTTGAGTCTACACAACCTCTGTGCAACATTAAAGTGCACATCTCCATCAGCTGTAAATTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCATTAGGACTGAAATCGGATGCACCCATGGATGTTATTTGGGATATTATGCGTTGCTGGGTCAAAAACCACCCAGTTAAAGCTCAACCAGAAGATCAGCCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAACACATCTCTCTTCTGGGTGCAGAGGCTGTAAATGGAGCTCTTAGCCATGCGGATTCTGAAGAGCCTAATGCAAAACGTCCCAAGGTAACAGAGGAAGAACCAGCTCCCAACCAGTAA

Coding sequence (CDS)

ATGGCGACAGATCTTAGCGAATTCACCATCACCAAGGAGGGAGAGGCTGAGATTCTCATGCACGCGAAAAATGAAGTCTTTTTCAATAAGACCCAGGTTAACAACAGAGACATATCCATTGCTGTCCTTAGGTCATTTATTACAAAACGCAAACGGGAGCATGAAGCAAAACTATCAAAAAGAGCAAAATCAGGGCAGAAGATTTCTGAAAATAATGGTTCTGAAACTGCTATGGAAGAAGCATCTAATGAAACTGCAACAAATGATGAAAAGTGCCATACAGATTGTGATGCTCATGAAAAACCATCGAATGAATGTGGAAGTCCAATGTTGTCGGAAGAACAAAAAGAGATCATAGAGGGAAAAGAACAAAGAGATCCCAAGCCAATTAGAGTTCTTGAGGCCTTGTCAGCTTCTGGGCTAAGAGCCTTAAGATATGCTCGTGAAGTAGAGGGGGTTGGTCAAGTTGTGGCGCTAGACAATGATAAAGCATCGGTTGAAGCTTGTCGGAGAAACATCAAGTTTAATGGTTCAGTAGCGTGTGCAAAAGTGGAATCACATCTAACTGATGCTCGTGTTTATATGCTCACTCACCCAAAAGAATTTGATATGGTGGATTTGGATCCTTACGGTTCACCTTCTATTTTCTTGGACTCTGCTGTTCAATCAGTTGTAGATGGTGGCATGCTGATGTGTACAGCAACTGATATGGCAGTGCTTTGTGGGGGAAATGGTGAAGTTTGCTACTCAAAATATGGTTCCTATCCTTTGAGGGGGAAATATTGCCATGAAATGGCTTTAAGAATCCTCCTTGCCTGCATTGAGAGCCATGCAAATAGATACAAGCGGTATATTGTTCCAGTGCTATCTGTCCAGATGGACTTCTATGTCCGGGTTTTTGTTCGTGTTTATACTTCTGCAAGTGCAATGAAAAATACTCCCCTTAAGCTCTCATATGTTTATCAGTGCACTGGTTGTGATTCTTTCCATCTCCAGCAGATTGGCAGAACCGTCTCCAAGAACAACAGCGTCAGGTATTTACCTGGTTTTGGTCCTTCTGTTGCTCAAGAGTGCAGTGATTGTGGGAAGAAATTCAACATGGGTGGGCCTATCTGGTCTGCTCCTATCCATGATCAAGAATGGGTTACTTCCATGCTAGCAGATGTGAAGTCTAGGAAGGACTCTTATCCTGCATATGATCGCATATCTGCTGTACTGACAACAATTTCAGAGGAGCTGATGGACGTTCCTTTATTTTTGAGTCTACACAACCTCTGTGCAACATTAAAGTGCACATCTCCATCAGCTGTAAATTTCCGTTCTGCAGTGATTAATGCAGGATATCGCATATCGGGCACTCATGTGAATCCATTAGGACTGAAATCGGATGCACCCATGGATGTTATTTGGGATATTATGCGTTGCTGGGTCAAAAACCACCCAGTTAAAGCTCAACCAGAAGATCAGCCAGGAAGTATCATTCTTGCCAAGGAACCAGTTCTTGAAGCTAATTTTGCTAGAGCTGTGGCATCTCTTAGCAAGGCACAAGCAAAGAAAATTGCACGCTTCCTTCCAAATCCTGAAAGACATTGGGGCCCCAAGCTGAGAGCAGGTCGTCAAATCACTAGTAAACACATCTCTCTTCTGGGTGCAGAGGCTGTAAATGGAGCTCTTAGCCATGCGGATTCTGAAGAGCCTAATGCAAAACGTCCCAAGGTAACAGAGGAAGAACCAGCTCCCAACCAGTAA

Protein sequence

MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSKRAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIEGKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ
Homology
BLAST of HG10018293 vs. NCBI nr
Match: XP_038890035.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida])

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 565/582 (97.08%), Postives = 573/582 (98.45%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLR FITKRK+EHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRLFITKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           RAKSGQK++ENNGSET MEEASNE+ATNDEKCHTDC AHEK SNECGSPMLSEEQKE+ E
Sbjct: 61  RAKSGQKVTENNGSETVMEEASNESATNDEKCHTDCGAHEKTSNECGSPMLSEEQKEVAE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQRDPKPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VR+YTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRIYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKRPKV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKV-EEEPAPNQ 581

BLAST of HG10018293 vs. NCBI nr
Match: XP_008459398.1 (PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo])

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 563/582 (96.74%), Postives = 571/582 (98.11%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFI KRK+EHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R+KSGQK+SENNGSETA EEA N +ATN+EKCHTDCDAHEKPSNECGSPMLSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKRPKV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKV-EEEPAPNQ 581

BLAST of HG10018293 vs. NCBI nr
Match: XP_011656015.1 (probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN52527.1 hypothetical protein Csa_008279 [Cucumis sativus])

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 563/582 (96.74%), Postives = 570/582 (97.94%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFI KRK+EHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R+KSGQK+SENNGSETA EEA NE+ATNDEKC TDCDAHEKPSNECGSPMLSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKR KV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKV-EEEPAPNQ 581

BLAST of HG10018293 vs. NCBI nr
Match: KAA0039424.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa] >TYK00612.1 putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 561/582 (96.39%), Postives = 569/582 (97.77%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           M TDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFI KRK+EHEAKLSK
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R+KSGQK+SENNGSETA EEA N +ATN+EKCHTDCDAHEKPS ECGSPMLSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKRPKV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKV-EEEPAPNQ 581

BLAST of HG10018293 vs. NCBI nr
Match: KAG6602539.1 (putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 548/582 (94.16%), Postives = 567/582 (97.42%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR+FI+KRK+EHE K+S+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           RAKS QKISENNGSE  MEEASNE+ATNDEKC+TDCDAHEK SNECGSP+L EEQKE +E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLPEEQKENVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGA+AVNGALSHADSEEPNAKR KV EE+PAPNQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKV-EEDPAPNQ 581

BLAST of HG10018293 vs. ExPASy Swiss-Prot
Match: Q9SRU7 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=At3g02320 PE=2 SV=3)

HSP 1 Score: 862.4 bits (2227), Expect = 2.8e-249
Identity = 431/581 (74.18%), Postives = 489/581 (84.17%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           M TDL+++T+ KEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F++KRK+EHEAK SK
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R +   K+ E + SE + EE  +E   N        +   + ++E G   +S++  +  E
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPSIFLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GR++ KNNSVRYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKS KD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+KAQ  +QPGS+IL+KEP  E +F+R + SLSKAQAKK+ARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPN 582
           ITSKH+SL+G EAVNG LS    E     + +  E EP  N
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE----LKEEDEEAEPEDN 569

BLAST of HG10018293 vs. ExPASy Swiss-Prot
Match: Q9LFU5 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g15810 PE=2 SV=3)

HSP 1 Score: 840.9 bits (2171), Expect = 8.8e-243
Identity = 424/578 (73.36%), Postives = 490/578 (84.78%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           M TDL+++T+ KEGEAE+LMH KN+VFFNK QVNNRD+SIAVLR+FI KRK+EHEA LSK
Sbjct: 1   METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           RA+S  K+ E + SET+ EE   E   ++ K + +   HE  + +       +E  +   
Sbjct: 61  RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
              +R+ KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 121 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PSIFLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 181 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GR++ KNNSVRYLPG GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKS KD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L ATLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 421 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  + PGS+IL+KEP  +A+F+R V SLSKAQAKK+ARFLPNPE+HWGPK+RAGR 
Sbjct: 481 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEP 579
           ITSKH+SLLG EAVNG L++ + +E   +  +  EEEP
Sbjct: 541 ITSKHVSLLGHEAVNGHLNN-NHKEAGDEEEEEEEEEP 569

BLAST of HG10018293 vs. ExPASy Swiss-Prot
Match: Q3TX08 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE=1 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 1.0e-105
Identity = 225/536 (41.98%), Postives = 325/536 (60.63%), Query Frame = 0

Query: 9   TITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREH-EAKLSKRAKSGQK 68
           T+T EG A+I+  + NEVF+N  Q  NRD++ AV    IT+  R H  AK  +    G+K
Sbjct: 57  TVT-EGAAKIVFPSANEVFYNPVQEFNRDLTCAV----ITEFARIHLGAKGIQIKVPGEK 116

Query: 69  ISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIEGKEQRDP 128
            SE    + + +E   ETA  +E                G    +    EI E       
Sbjct: 117 DSEKIAVDLSDQE--EETAGKNENLAP------------GDWPRTAAVGEICE------- 176

Query: 129 KPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESH 188
           + +RVLE L+ASGLR++R+A EV G+  VVA D    +VE   RN++ NG      V+ +
Sbjct: 177 EGLRVLEGLAASGLRSIRFALEVPGLQSVVANDASARAVELMHRNVELNG--VAHLVQPN 236

Query: 189 LTDARVYMLTH---PKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGN 248
             DAR+ M  H   P+ FD++DLDPYGSP+ FLD+AVQ+V DGG+L  T TDMAVL G +
Sbjct: 237 QADARMLMYQHQKAPERFDVIDLDPYGSPAPFLDAAVQAVSDGGLLCVTCTDMAVLAGNS 296

Query: 249 GEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVY 308
           GE CYSKYG+  L+ + CHEMALRI+L  ++ HAN Y+RYIVP+LS+  DFY+RVFVRV+
Sbjct: 297 GETCYSKYGAMALKSRACHEMALRIVLHSLDLHANCYQRYIVPLLSISADFYIRVFVRVF 356

Query: 309 TSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR-TVSKNNSVRYLPGFGPSVAQECSDCGK 368
           T  + +K++  K + V+QC GC +F+LQ++G+ +      +++    GP V  EC  CG+
Sbjct: 357 TGQAKVKSSASKQALVFQCVGCGAFYLQRLGKASGDPGGRIKFSAACGPPVTPECEHCGQ 416

Query: 369 KFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFLSLH 428
           +  +GGP+W+ PIHD ++V  +L  V +    +    RI  VL+ ++EEL DVPL+ +L 
Sbjct: 417 RHQLGGPMWAEPIHDLDFVGRVLDAVTTNPGRFHTSMRIQGVLSVVTEELPDVPLYYTLD 476

Query: 429 NLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPVK 488
            L +T+ C +P  +  RSA+++AG+R+S +H     +K+DAP + +WDIMRCW K  PVK
Sbjct: 477 QLSSTIHCNTPRLLQLRSALLHAGFRVSLSHACKNAVKTDAPPEALWDIMRCWEKECPVK 536

Query: 489 AQ--PEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRA 538
            +   E  P   ILA EP L+ANF     +   ++ + + RF  NPE +WGP+ RA
Sbjct: 537 RERLSESSPAFRILAVEPRLKANFNIREDANPSSRQRGLKRFQANPEANWGPRPRA 564

BLAST of HG10018293 vs. ExPASy Swiss-Prot
Match: Q9NXH9 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=1 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 3.6e-103
Identity = 216/541 (39.93%), Postives = 321/541 (59.33%), Query Frame = 0

Query: 4   DLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSKRAK 63
           ++ E T+T EG A+I   + NEVF+N  Q  NRD++ AV+  F         A++   AK
Sbjct: 51  EVQETTVT-EGAAKIAFPSANEVFYNPVQEFNRDLTCAVITEF---------ARIQLGAK 110

Query: 64  SGQ-KISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIEGK 123
             Q K+     ++  + + S +    +EK     +  E  +   G    +    EI E  
Sbjct: 111 GIQIKVPGEKDTQKVVVDLSEQ---EEEK----VELKESENLASGDQPRTAAVGEICE-- 170

Query: 124 EQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACA 183
                + + VLE L+ASGLR++R+A EV G+  VVA D    +V+  RRN++ N      
Sbjct: 171 -----EGLHVLEGLAASGLRSIRFALEVPGLRSVVANDASTRAVDLIRRNVQLND--VAH 230

Query: 184 KVESHLTDARVYMLTHPK---EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAV 243
            V+    DAR+ M  H +    FD++DLDPYGSP+ FLD+AVQ+V +GG+L  T TDMAV
Sbjct: 231 LVQPSQADARMLMYQHQRVSERFDVIDLDPYGSPATFLDAAVQAVSEGGLLCVTCTDMAV 290

Query: 244 LCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRV 303
           L G +GE CYSKYG+  L+ + CHEMALRI+L  ++  AN Y+R++VP+LS+  DFYVRV
Sbjct: 291 LAGNSGETCYSKYGAMALKSRACHEMALRIVLHSLDLRANCYQRFVVPLLSISADFYVRV 350

Query: 304 FVRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVS-KNNSVRYLPGFGPSVAQEC 363
           FVRV+T  + +K +  K + V+QC GC +FHLQ++G+     +   ++    GP V  EC
Sbjct: 351 FVRVFTGQAKVKASASKQALVFQCVGCGAFHLQRLGKASGVPSGRAKFSAACGPPVTPEC 410

Query: 364 SDCGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPL 423
             CG++  +GGP+W+ PIHD ++V  +L  V +    +   +RI  VL+ I+EEL DVPL
Sbjct: 411 EHCGQRHQLGGPMWAEPIHDLDFVGRVLEAVSANPGRFHTSERIRGVLSVITEELPDVPL 470

Query: 424 FLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVK 483
           + +L  L +T+ C +PS +  RSA+++A +R+S +H     +K+DAP   +WDIMRCW K
Sbjct: 471 YYTLDQLSSTIHCNTPSLLQLRSALLHADFRVSLSHACKNAVKTDAPASALWDIMRCWEK 530

Query: 484 NHPVKAQ--PEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLR 538
             PVK +   E  P   IL+ EP L+ANF     +   ++ + + RF  NPE +WGP+ R
Sbjct: 531 ECPVKRERLSETSPAFRILSVEPRLQANFTIREDANPSSRQRGLKRFQANPEANWGPRPR 565

BLAST of HG10018293 vs. ExPASy Swiss-Prot
Match: Q9VK89 (Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster OX=7227 GN=CG6388 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 1.4e-99
Identity = 220/538 (40.89%), Postives = 302/538 (56.13%), Query Frame = 0

Query: 10  ITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSKRAKSGQKIS 69
           + +E  AEI+  +   VF+N  Q  NRD+SIA L  +  +  +E   K  K  K  +K+ 
Sbjct: 19  VIRERNAEIV--SGGNVFYNPVQEFNRDLSIAALNVYRQRLTKERSEKALK--KQRKKVK 78

Query: 70  ENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIEGKEQRDPKP 129
           E                  DEK  T     + P  E G+                R    
Sbjct: 79  EQ----------------EDEK--TTPVPEDPPVYEAGT----------------RYEDG 138

Query: 130 IRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAKVESHLT 189
           +R+LEAL+A+GLR++RYA+E+ GV Q+VA D  + +V +   NI+ N      +      
Sbjct: 139 LRILEALAATGLRSIRYAQEIAGVRQIVANDLSRQAVASINTNIRHNKVEELIEPSHSDA 198

Query: 190 DARVYMLTHP-KEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVLCGGNGEVC 249
              +Y+ T P K FD VDLDPYG P+ FLD A+Q +VDGG+L+ TATDMAVL G   E C
Sbjct: 199 MTLMYLSTQPEKRFDAVDLDPYGCPNRFLDGAMQCLVDGGLLLVTATDMAVLAGNAPEAC 258

Query: 250 YSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVFVRVYTSAS 309
           Y KYGS PLR K CHEMALRILL CIESHANRY +YI P+LS+  DFY+R+FVRVY   +
Sbjct: 259 YVKYGSVPLRMKCCHEMALRILLHCIESHANRYGKYIEPLLSISADFYIRIFVRVYVGQA 318

Query: 310 AMKNTPLKLSYVYQCTGCDSFHLQQIG-----RTVSKNNSVRYLPGFGPSVAQECSDCGK 369
             K +  K S++YQCTGC++F LQ +G      T      +++    GP+V  +C  CG 
Sbjct: 319 QCKLSMSKQSWIYQCTGCETFTLQPLGITKPNPTAGNPQQLKFGIPTGPAVNSQCEHCGH 378

Query: 370 KFNMGGPIWSAPIHDQEWVTSMLADV-KSRKDSYPAYDRISAVLTTISEELMDVPLFLSL 429
           + ++GGPIWSAPIH+ E+V  +L  V ++   S     RI  VL+ + EEL DVPL+ + 
Sbjct: 379 RHHLGGPIWSAPIHNPEFVQDLLTAVQETTLQSLGTQRRIVGVLSMVQEELQDVPLYYTP 438

Query: 430 HNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNHPV 489
             LC  LK      + FRSA+++AGYR+S +H +   LK++AP  V+WDI+R W K HPV
Sbjct: 439 DKLCCVLKLEIVPMLKFRSAILHAGYRVSYSHASKNSLKTNAPPAVLWDILRSWSKRHPV 498

Query: 490 KAQP--EDQPGSIILAKEPVLEANFARAVASLS-KAQAKKIARFLPNPERHWGPKLRA 538
             +      P + IL+KE      F       + K++   ++RF  NP  HWGP  RA
Sbjct: 499 NPERMIPGSPLAAILSKECTAVYEFDELHPEANPKSRKSALSRFQENPTPHWGPGTRA 518

BLAST of HG10018293 vs. ExPASy TrEMBL
Match: A0A1S3CA23 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498546 PE=3 SV=1)

HSP 1 Score: 1119.0 bits (2893), Expect = 0.0e+00
Identity = 563/582 (96.74%), Postives = 571/582 (98.11%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFI KRK+EHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R+KSGQK+SENNGSETA EEA N +ATN+EKCHTDCDAHEKPSNECGSPMLSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKRPKV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKV-EEEPAPNQ 581

BLAST of HG10018293 vs. ExPASy TrEMBL
Match: A0A0A0KVU4 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G641600 PE=3 SV=1)

HSP 1 Score: 1117.1 bits (2888), Expect = 0.0e+00
Identity = 563/582 (96.74%), Postives = 570/582 (97.94%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFI KRK+EHEAKLSK
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R+KSGQK+SENNGSETA EEA NE+ATNDEKC TDCDAHEKPSNECGSPMLSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEACNESATNDEKCDTDCDAHEKPSNECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C+KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CSKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVK QPEDQ GSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKTQPEDQAGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKR KV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRSKV-EEEPAPNQ 581

BLAST of HG10018293 vs. ExPASy TrEMBL
Match: A0A5A7T7H3 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002310 PE=3 SV=1)

HSP 1 Score: 1115.1 bits (2883), Expect = 0.0e+00
Identity = 561/582 (96.39%), Postives = 569/582 (97.77%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           M TDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFI KRK+EHEAKLSK
Sbjct: 1   MTTDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFIAKRKQEHEAKLSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R+KSGQK+SENNGSETA EEA N +ATN+EKCHTDCDAHEKPS ECGSPMLSEEQKEI+E
Sbjct: 61  RSKSGQKLSENNGSETATEEAFNGSATNEEKCHTDCDAHEKPSTECGSPMLSEEQKEIVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKEQR PKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEAC+RNIKFNGSVA
Sbjct: 121 GKEQRGPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           C KVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CTKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSVAQECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVAQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KDSYPAYDRISAVLTTISEEL DVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDSYPAYDRISAVLTTISEELNDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSH DSEEPNAKRPKV EEEPAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHTDSEEPNAKRPKV-EEEPAPNQ 581

BLAST of HG10018293 vs. ExPASy TrEMBL
Match: A0A6J1GSN6 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111456770 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 547/582 (93.99%), Postives = 567/582 (97.42%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR+FI+KRK+EHE K+S+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           RAKS QKISENNGSE  MEEASNE+ATNDEKC+TDCDAHEK SNECGSP+LSEEQKE + 
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAHEKLSNECGSPVLSEEQKENVA 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWV+SMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVSSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGA+AVNGALSHADSEEPNAKR KV EE+PAPNQ
Sbjct: 541 ITSKHISLLGADAVNGALSHADSEEPNAKRSKV-EEDPAPNQ 581

BLAST of HG10018293 vs. ExPASy TrEMBL
Match: A0A6J1JNF2 (tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111487439 PE=3 SV=1)

HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 548/582 (94.16%), Postives = 565/582 (97.08%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           MATDLSEFTI KEGEAEILMHAKNEVFFNKTQVNNRD+SIAVLR+FI+KRK+EHE K+S+
Sbjct: 1   MATDLSEFTIIKEGEAEILMHAKNEVFFNKTQVNNRDLSIAVLRAFISKRKQEHETKISR 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           RAKS QKISENNGSE  MEEASNE+ATNDEKC+TDCDA EK SNECGSPML E QKE +E
Sbjct: 61  RAKSAQKISENNGSEIVMEEASNESATNDEKCNTDCDAREKLSNECGSPMLPEGQKENVE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
           GKE+RD KPIRVLEALSASGLRALRYAREVEG+GQVVALDNDKASVEACRRNIKFNGS+A
Sbjct: 121 GKERRDAKPIRVLEALSASGLRALRYAREVEGIGQVVALDNDKASVEACRRNIKFNGSIA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
           CAKVESHLTDARVYMLTHP EFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL
Sbjct: 181 CAKVESHLTDARVYMLTHPNEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGR+VSKNNSVRYLPGFGPSV QECSD
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRSVSKNNSVRYLPGFGPSVPQECSD 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS KD+YPAYDRISAVLTTISEELMDVPLFL
Sbjct: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSMKDTYPAYDRISAVLTTISEELMDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPM VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMSVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           PVKAQPEDQPGSIILAKEPVLEANFARA+ASLSKAQAKK+ARFLPNPERHWGPKLRAGRQ
Sbjct: 481 PVKAQPEDQPGSIILAKEPVLEANFARAIASLSKAQAKKVARFLPNPERHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPNQ 583
           ITSKHISLLGAEAVNGALSHADSEEPNAKR KV EE+PAPNQ
Sbjct: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRSKV-EEDPAPNQ 581

BLAST of HG10018293 vs. TAIR 10
Match: AT3G02320.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 862.4 bits (2227), Expect = 2.0e-250
Identity = 431/581 (74.18%), Postives = 489/581 (84.17%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           M TDL+++T+ KEGEAEILMH KN+VFFNK QVNNRD+SIAVLR F++KRK+EHEAK SK
Sbjct: 1   METDLNDYTVIKEGEAEILMHKKNQVFFNKAQVNNRDMSIAVLREFLSKRKQEHEAKSSK 60

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           R +   K+ E + SE + EE  +E   N        +   + ++E G   +S++  +  E
Sbjct: 61  RTRPASKVIEKDASEASKEETPSENGMN--------NGDHEVASEDGPSSVSKDPAKTTE 120

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
               R+PKP +VLEALSASGLRALRYARE+EG+GQVVALDND ASVEAC+RNIKFNGSVA
Sbjct: 121 RFAPREPKPPKVLEALSASGLRALRYAREIEGIGQVVALDNDLASVEACQRNIKFNGSVA 180

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESH TDARV+MLTHPKEFD+VDLDPYGSPSIFLDSA+QSV DGG+LMCTATDMAVL
Sbjct: 181 ISKVESHHTDARVHMLTHPKEFDVVDLDPYGSPSIFLDSAIQSVTDGGLLMCTATDMAVL 240

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CGGNGEVCYSKYGSYPLR KYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 241 CGGNGEVCYSKYGSYPLRAKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 300

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GR++ KNNSVRYLP  GP V Q+C+ 
Sbjct: 301 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQPVGRSLPKNNSVRYLPAIGPVVKQDCNH 360

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAP+HD EWVTS+L  VKS KD YPAYDRISAVLTT+SEEL+DVPLFL
Sbjct: 361 CGKKYNMGGPIWSAPMHDPEWVTSILNSVKSMKDRYPAYDRISAVLTTVSEELLDVPLFL 420

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLHNLCATLKC SPSA  FRSAVINA YRISGTHVNPLG+K+DAPM+VIWDIMRCWVKNH
Sbjct: 421 SLHNLCATLKCISPSAAMFRSAVINANYRISGTHVNPLGMKTDAPMEVIWDIMRCWVKNH 480

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+KAQ  +QPGS+IL+KEP  E +F+R + SLSKAQAKK+ARFLPNPE+HWGPKLRAGRQ
Sbjct: 481 PIKAQSPEQPGSVILSKEPSHEVDFSRHIGSLSKAQAKKVARFLPNPEKHWGPKLRAGRQ 540

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEPAPN 582
           ITSKH+SL+G EAVNG LS    E     + +  E EP  N
Sbjct: 541 ITSKHVSLIGHEAVNGHLSQHHEE----LKEEDEEAEPEDN 569

BLAST of HG10018293 vs. TAIR 10
Match: AT5G15810.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 840.9 bits (2171), Expect = 6.3e-244
Identity = 424/578 (73.36%), Postives = 490/578 (84.78%), Query Frame = 0

Query: 1   MATDLSEFTITKEGEAEILMHAKNEVFFNKTQVNNRDISIAVLRSFITKRKREHEAKLSK 60
           M TDL+++T+ KEGEAE+LMH KN+VFFNK QVNNRD+SIAVLR+FI KRK+EHEA LSK
Sbjct: 99  METDLNDYTVIKEGEAEVLMHKKNQVFFNKAQVNNRDMSIAVLRAFIIKRKQEHEAMLSK 158

Query: 61  RAKSGQKISENNGSETAMEEASNETATNDEKCHTDCDAHEKPSNECGSPMLSEEQKEIIE 120
           RA+S  K+ E + SET+ EE   E   ++ K + +   HE  + +       +E  +   
Sbjct: 159 RARSSGKVVEKDVSETSKEETPTENGDDNGKTNGE---HEVTTQDG-----PKEAAKTAY 218

Query: 121 GKEQRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVA 180
              +R+ KP RVLEALSASGLRALRYAREVEG+GQVVALDND ASVEAC+RNIKFNG ++
Sbjct: 219 ESARRELKPPRVLEALSASGLRALRYAREVEGIGQVVALDNDPASVEACQRNIKFNGLMS 278

Query: 181 CAKVESHLTDARVYMLTHPKEFDMVDLDPYGSPSIFLDSAVQSVVDGGMLMCTATDMAVL 240
            +KVESHLTDARV+ML+HPK+FD+VDLDPYG+PSIFLDSAVQSV DGG+LMCTATDMAVL
Sbjct: 279 TSKVESHLTDARVHMLSHPKDFDVVDLDPYGAPSIFLDSAVQSVADGGLLMCTATDMAVL 338

Query: 241 CGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLSVQMDFYVRVF 300
           CG NGEVCYSKYGSYPL+GKYCHEMALRILLA IESHANRYKRYIVPVLSVQMDFYVRVF
Sbjct: 339 CGANGEVCYSKYGSYPLKGKYCHEMALRILLASIESHANRYKRYIVPVLSVQMDFYVRVF 398

Query: 301 VRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGFGPSVAQECSD 360
           VRVYTSASAMKNTPLKLSYVYQC GCDSFHLQ +GR++ KNNSVRYLPG GP V Q+C+ 
Sbjct: 399 VRVYTSASAMKNTPLKLSYVYQCIGCDSFHLQSVGRSLPKNNSVRYLPGVGPVVPQDCTH 458

Query: 361 CGKKFNMGGPIWSAPIHDQEWVTSMLADVKSRKDSYPAYDRISAVLTTISEELMDVPLFL 420
           CGKK+NMGGPIWSAPIHDQEWV S+L  VKS KD YPAYDRI AVLTTISEEL DVPLFL
Sbjct: 459 CGKKYNMGGPIWSAPIHDQEWVNSILNGVKSMKDRYPAYDRICAVLTTISEELPDVPLFL 518

Query: 421 SLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDAPMDVIWDIMRCWVKNH 480
           SLH+L ATLKCTSPSA  FRSAVINA YR+SG+HVNPLG+K+DAPM++IWDIMRCWVKNH
Sbjct: 519 SLHSLSATLKCTSPSAALFRSAVINAKYRVSGSHVNPLGIKTDAPMEIIWDIMRCWVKNH 578

Query: 481 PVKAQPEDQPGSIILAKEPVLEANFARAVASLSKAQAKKIARFLPNPERHWGPKLRAGRQ 540
           P+K Q  + PGS+IL+KEP  +A+F+R V SLSKAQAKK+ARFLPNPE+HWGPK+RAGR 
Sbjct: 579 PIKPQSPEHPGSVILSKEPSHQADFSRHVGSLSKAQAKKVARFLPNPEKHWGPKIRAGRT 638

Query: 541 ITSKHISLLGAEAVNGALSHADSEEPNAKRPKVTEEEP 579
           ITSKH+SLLG EAVNG L++ + +E   +  +  EEEP
Sbjct: 639 ITSKHVSLLGHEAVNGHLNN-NHKEAGDEEEEEEEEEP 667

BLAST of HG10018293 vs. TAIR 10
Match: AT3G56330.1 (N2,N2-dimethylguanosine tRNA methyltransferase )

HSP 1 Score: 95.9 bits (237), Expect = 1.1e-19
Identity = 91/362 (25.14%), Postives = 151/362 (41.71%), Query Frame = 0

Query: 124 QRDPKPIRVLEALSASGLRALRYAREVEGVGQVVALDNDKASVEACRRNIKFNGSVACAK 183
           +R    +RVL+A+   G+R+LRY  E E    V+A D +  +    RR I  N     +K
Sbjct: 84  KRSNGSLRVLDAMCGCGIRSLRYLVEAE-ADFVMANDANDDN----RRVITDN----LSK 143

Query: 184 VESHLTDARVYMLTH-------------PKEFDMVDLDPYGSPSIFLDSAVQSVVDGGML 243
           VE    D R +++TH                FDM+D+D +GS S FL  A  ++  GG+L
Sbjct: 144 VERGTGDERRWVVTHMLANKAMIERYMVADFFDMIDIDSFGSDSSFLRDAFNALRLGGLL 203

Query: 244 MCTATDMAVLCGGNGEVCYSKYGSYPLRGKYCHEMALRILLACIESHANRYKRYIVPVLS 303
             T+TD     G       + YG++     + +E+ LR+L+      A     ++ P+ S
Sbjct: 204 YLTSTDGYSSGGHRPYNSLAAYGAFIRPMPFGNEIGLRMLIGGAVREAALLGYHVTPLFS 263

Query: 304 VQMDFYVRVFVRVYTSASAMKNTPLKLSYVYQCTGCDSFHLQQIGRTVSKNNSVRYLPGF 363
               ++  VF  +        +      +V  C  C   H  +                 
Sbjct: 264 -YYSYHGPVFRVMLRVHRGKLHEDRNYGFVTHCNLCGHSHTLRFDEL------------- 323

Query: 364 GPSVAQECSD--CGKKFNMGGPIWSAPIHDQEWVTSMLADVKS----RKDSYPAYDRISA 423
              +   CSD        + GP+W  P+HD  +VT ML   K      + +    D++ +
Sbjct: 324 -GLMGCPCSDTKASSSLVVSGPMWLGPLHDASYVTEMLELAKEWGWVSEGTGMDLDKLLS 383

Query: 424 VLTTISEELMDVPLFLSLHNLCATLKCTSPSAVNFRSAVINAGYRISGTHVNPLGLKSDA 467
           ++   S+  +  P +  +  + +  K  SP      SA++  GY  S +H+ P  LK+D 
Sbjct: 384 IMIEESDPRLP-PGYTKMDEMASRAKMNSPPLKKMMSALVKEGYAASRSHIIPNALKTDC 420

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890035.10.0e+0097.08probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Benincasa hispida][more]
XP_008459398.10.0e+0096.74PREDICTED: probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo][more]
XP_011656015.10.0e+0096.74probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis sativus] >KGN525... [more]
KAA0039424.10.0e+0096.39putative tRNA (guanine(26)-N(2))-dimethyltransferase 2 [Cucumis melo var. makuwa... [more]
KAG6602539.10.0e+0094.16putative tRNA (guanine(26)-N(2))-dimethyltransferase 2, partial [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
Q9SRU72.8e-24974.18Probable tRNA (guanine(26)-N(2))-dimethyltransferase 2 OS=Arabidopsis thaliana O... [more]
Q9LFU58.8e-24373.36Probable tRNA (guanine(26)-N(2))-dimethyltransferase 1 OS=Arabidopsis thaliana O... [more]
Q3TX081.0e-10541.98tRNA (guanine(26)-N(2))-dimethyltransferase OS=Mus musculus OX=10090 GN=Trmt1 PE... [more]
Q9NXH93.6e-10339.93tRNA (guanine(26)-N(2))-dimethyltransferase OS=Homo sapiens OX=9606 GN=TRMT1 PE=... [more]
Q9VK891.4e-9940.89Probable tRNA (guanine(26)-N(2))-dimethyltransferase OS=Drosophila melanogaster ... [more]
Match NameE-valueIdentityDescription
A0A1S3CA230.0e+0096.74tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A0A0KVU40.0e+0096.74tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_5G... [more]
A0A5A7T7H30.0e+0096.39tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A6J1GSN60.0e+0093.99tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JNF20.0e+0094.16tRNA (guanine(26)-N(2))-dimethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G02320.12.0e-25074.18N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT5G15810.16.3e-24473.36N2,N2-dimethylguanosine tRNA methyltransferase [more]
AT3G56330.11.1e-1925.14N2,N2-dimethylguanosine tRNA methyltransferase [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002905tRNA methyltransferase, Trm1TIGRFAMTIGR00308TIGR00308coord: 12..475
e-value: 1.7E-96
score: 321.8
IPR002905tRNA methyltransferase, Trm1PFAMPF02005TRMcoord: 15..474
e-value: 2.1E-120
score: 402.4
IPR002905tRNA methyltransferase, Trm1PANTHERPTHR10631N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASEcoord: 1..564
IPR002905tRNA methyltransferase, Trm1PROSITEPS51626SAM_MT_TRM1coord: 9..473
score: 137.532074
IPR042296tRNA methyltransferase, Trm1, C-terminalGENE3D3.30.56.70coord: 417..475
e-value: 2.1E-21
score: 77.4
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 9..415
e-value: 8.4E-124
score: 414.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..83
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 557..582
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 84..102
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 53..111
NoneNo IPR availablePANTHERPTHR10631:SF12TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE 1-RELATEDcoord: 1..564
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 8..475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10018293.1HG10018293.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002940 tRNA N2-guanine methylation
biological_process GO:0008033 tRNA processing
cellular_component GO:0005634 nucleus
molecular_function GO:0000049 tRNA binding
molecular_function GO:0004809 tRNA (guanine-N2-)-methyltransferase activity
molecular_function GO:0003723 RNA binding