HG10018256 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10018256 vs. NCBI nr
Match: XP_038894011.1 (berberine bridge enzyme-like 18 isoform X2 [Benincasa hispida])

HSP 1 Score: 952.2 bits (2460), Expect = 2.0e-273
Identity = 466/562 (82.92%), Postives = 505/562 (89.86%), Query Frame = 0

Query: 1   MEYFWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSS 60
           M+YFWS   YY FISLLL  SLATLG  GDN EDFV+CI LHSSNNNDSN+TLIHTPSSS
Sbjct: 1   MKYFWS--SYYSFISLLLLLSLATLGCWGDNGEDFVHCISLHSSNNNDSNTTLIHTPSSS 60

Query: 61  SYSYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEG 120
           SYSYVLNFSIRNLRFSEPET KP+AIITPSHAS VQA VICCKSHGLQ+RTRSGGHDFEG
Sbjct: 61  SYSYVLNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEG 120

Query: 121 RSYVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPT 180
           RSYVANVPFVLIDL+NLNSITIDTEDESAWVQSGAT+G+LYYRI EKSQTLGFPAGFAPT
Sbjct: 121 RSYVANVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPT 180

Query: 181 IGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSF 240
           IGVGG+LSGGG GMMVRKYGLA DNVVDAYVVDANGR+ +R+SMGEDLFWAIRGGGGGSF
Sbjct: 181 IGVGGYLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSF 240

Query: 241 GIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSS 300
           GIVLAWKLKLVPVPPIVTSFALH+TWDQNAA LIH WQY+APEVD+DLFI+AWVTASNSS
Sbjct: 241 GIVLAWKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTASNSS 300

Query: 301 NGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTE 360
             G RIMEASF SLFLGKA +LL+LM+KTFPELGLKKEDCLEMSW+E+MAF ASGFVS  
Sbjct: 301 KEGGRIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAFMASGFVSAN 360

Query: 361 SLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNE 420
           +++LLLDRTP  N R+K KSDYA +PIS+TALEGMWERFKIEELETVQL+LIPFGGKMNE
Sbjct: 361 NVKLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFKIEELETVQLMLIPFGGKMNE 420

Query: 421 ISKAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYR 480
           IS+ ETP  YRAGYPI IGYYVTWQ P+AD +HL+WT+ELHDYMTPFVSKSPR AY+NY+
Sbjct: 421 ISETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINYK 480

Query: 481 DLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRV 540
           DLDIGTN EDGIP SYEEA  WGH YFGNNFERL+EVKMKVDPFNFFRHEQSIPP     
Sbjct: 481 DLDIGTNKEDGIPTSYEEAGVWGHPYFGNNFERLVEVKMKVDPFNFFRHEQSIPP----- 540

Query: 541 QGIKAKMGSCLDVPYETMSCAI 563
               AKM SC DVP+ET+  A+
Sbjct: 541 ----AKMASCPDVPHETLPNAM 551

BLAST of HG10018256 vs. NCBI nr
Match: XP_004147307.2 (berberine bridge enzyme-like 27 [Cucumis sativus])

HSP 1 Score: 950.7 bits (2456), Expect = 5.9e-273
Identity = 469/541 (86.69%), Postives = 498/541 (92.05%), Query Frame = 0

Query: 1   MEYFWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSS 60
           M+YFW   YYY FISLLL  SLATLG G +N++DFVNCIY+HSSNNNDSNSTLIHTPSSS
Sbjct: 1   MKYFWK-CYYYSFISLLLL-SLATLGCGSNNMKDFVNCIYVHSSNNNDSNSTLIHTPSSS 60

Query: 61  SYSYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEG 120
           SYSYVLNFSIRNLRFSEPET KP+AIITPSHASQVQATVICCKSHGLQ+RTRSGGHDFEG
Sbjct: 61  SYSYVLNFSIRNLRFSEPETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDFEG 120

Query: 121 RSYVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPT 180
           RSYVANVPFVLIDL+NLNSITID EDESAWVQSGATVGELY+RIGEKS+TLGFPAGFA T
Sbjct: 121 RSYVANVPFVLIDLINLNSITIDVEDESAWVQSGATVGELYFRIGEKSRTLGFPAGFAAT 180

Query: 181 IGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSF 240
           IG+GGFLSGGG GMMVRKYGL ADNVVDAYVVD NGRVVNRYSMGEDLFWAIRGGGGGSF
Sbjct: 181 IGLGGFLSGGGFGMMVRKYGLGADNVVDAYVVDGNGRVVNRYSMGEDLFWAIRGGGGGSF 240

Query: 241 GIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSS 300
           GIVLAWKL+LV VP IVTSFALH+ WDQNAA LI+ WQYIAP VDQDLFISAWVTASNSS
Sbjct: 241 GIVLAWKLRLVQVPSIVTSFALHKIWDQNAANLIYRWQYIAPWVDQDLFISAWVTASNSS 300

Query: 301 NGGE-RIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVST 360
           + G  RIMEASF SLFLG ATELL+LMEKTFPELGLKKEDCLE SWVE+MAFSASGFVS 
Sbjct: 301 HDGSGRIMEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSA 360

Query: 361 ESLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMN 420
           +SLELLLDRTP  NGR+K KSDYA EPISET LEGMWERFK EELETVQLILIPFGGK N
Sbjct: 361 KSLELLLDRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTN 420

Query: 421 EISKAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNY 480
           EIS++ETPSP+RAGYPIHIGYY+TWQRP+ADSKHL+W RELH+YMTPFVSKSPR AYVNY
Sbjct: 421 EISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNY 480

Query: 481 RDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR 540
           RDLD+GTNN+DG+P   EEAS WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP  
Sbjct: 481 RDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPTS 539

BLAST of HG10018256 vs. NCBI nr
Match: XP_038894010.1 (berberine bridge enzyme-like 18 isoform X1 [Benincasa hispida])

HSP 1 Score: 947.6 bits (2448), Expect = 5.0e-272
Identity = 466/563 (82.77%), Postives = 505/563 (89.70%), Query Frame = 0

Query: 1   MEYFWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSS 60
           M+YFWS   YY FISLLL  SLATLG  GDN EDFV+CI LHSSNNNDSN+TLIHTPSSS
Sbjct: 1   MKYFWS--SYYSFISLLLLLSLATLGCWGDNGEDFVHCISLHSSNNNDSNTTLIHTPSSS 60

Query: 61  SYSYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEG 120
           SYSYVLNFSIRNLRFSEPET KP+AIITPSHAS VQA VICCKSHGLQ+RTRSGGHDFEG
Sbjct: 61  SYSYVLNFSIRNLRFSEPETPKPVAIITPSHASHVQAAVICCKSHGLQIRTRSGGHDFEG 120

Query: 121 RSYVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPT 180
           RSYVANVPFVLIDL+NLNSITIDTEDESAWVQSGAT+G+LYYRI EKSQTLGFPAGFAPT
Sbjct: 121 RSYVANVPFVLIDLINLNSITIDTEDESAWVQSGATIGKLYYRIAEKSQTLGFPAGFAPT 180

Query: 181 IGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSF 240
           IGVGG+LSGGG GMMVRKYGLA DNVVDAYVVDANGR+ +R+SMGEDLFWAIRGGGGGSF
Sbjct: 181 IGVGGYLSGGGFGMMVRKYGLAVDNVVDAYVVDANGRIFDRHSMGEDLFWAIRGGGGGSF 240

Query: 241 GIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVT-ASNS 300
           GIVLAWKLKLVPVPPIVTSFALH+TWDQNAA LIH WQY+APEVD+DLFI+AWVT ASNS
Sbjct: 241 GIVLAWKLKLVPVPPIVTSFALHKTWDQNAADLIHRWQYVAPEVDEDLFITAWVTAASNS 300

Query: 301 SNGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVST 360
           S  G RIMEASF SLFLGKA +LL+LM+KTFPELGLKKEDCLEMSW+E+MAF ASGFVS 
Sbjct: 301 SKEGGRIMEASFFSLFLGKADKLLSLMQKTFPELGLKKEDCLEMSWIESMAFMASGFVSA 360

Query: 361 ESLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMN 420
            +++LLLDRTP  N R+K KSDYA +PIS+TALEGMWERFKIEELETVQL+LIPFGGKMN
Sbjct: 361 NNVKLLLDRTPLSNDRYKTKSDYATKPISKTALEGMWERFKIEELETVQLMLIPFGGKMN 420

Query: 421 EISKAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNY 480
           EIS+ ETP  YRAGYPI IGYYVTWQ P+AD +HL+WT+ELHDYMTPFVSKSPR AY+NY
Sbjct: 421 EISETETPFAYRAGYPIQIGYYVTWQDPDADLRHLKWTQELHDYMTPFVSKSPRGAYINY 480

Query: 481 RDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPR 540
           +DLDIGTN EDGIP SYEEA  WGH YFGNNFERL+EVKMKVDPFNFFRHEQSIPP    
Sbjct: 481 KDLDIGTNKEDGIPTSYEEAGVWGHPYFGNNFERLVEVKMKVDPFNFFRHEQSIPP---- 540

Query: 541 VQGIKAKMGSCLDVPYETMSCAI 563
                AKM SC DVP+ET+  A+
Sbjct: 541 -----AKMASCPDVPHETLPNAM 552

BLAST of HG10018256 vs. NCBI nr
Match: XP_038895067.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 937.2 bits (2421), Expect = 6.7e-269
Identity = 466/541 (86.14%), Postives = 494/541 (91.31%), Query Frame = 0

Query: 1   MEYFWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSS 60
           M+YF S   YY FI LLLF SLATLG  G N+EDFV+CIYLH SNNNDSNSTLIHTPSSS
Sbjct: 1   MKYFCS--CYYGFIFLLLF-SLATLGCWGYNMEDFVHCIYLH-SNNNDSNSTLIHTPSSS 60

Query: 61  SYSYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEG 120
           SYSYVLNFSIRNLRFSEPET KP+AIITPSHASQVQA VICCKSHG Q+RTRSGGHDFEG
Sbjct: 61  SYSYVLNFSIRNLRFSEPETPKPVAIITPSHASQVQAAVICCKSHGFQIRTRSGGHDFEG 120

Query: 121 RSYVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPT 180
            SYVANVPFVLIDL+NLNSITIDTEDESAWVQSGATVGELYYRIGEKS+TLGFPAGF PT
Sbjct: 121 SSYVANVPFVLIDLINLNSITIDTEDESAWVQSGATVGELYYRIGEKSRTLGFPAGFVPT 180

Query: 181 IGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSF 240
           IG+GGFLSGGG GMMVRKYGLAADNVVDAYVVDANG V +RYSMGEDLFWAIRGGGGGSF
Sbjct: 181 IGIGGFLSGGGFGMMVRKYGLAADNVVDAYVVDANGSVFDRYSMGEDLFWAIRGGGGGSF 240

Query: 241 GIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSS 300
           GIVLAWKLKLVPVPP VTSF+L +TWDQNAA LIH WQY+APEVDQDLFISAWVTA NSS
Sbjct: 241 GIVLAWKLKLVPVPPTVTSFSLSKTWDQNAADLIHRWQYVAPEVDQDLFISAWVTAFNSS 300

Query: 301 NGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTE 360
           +G  RIMEASF SL+LGKATELL+LMEKTFPELGLKKEDCLEMSWVE+MAFSASGFVS +
Sbjct: 301 DGSGRIMEASFFSLYLGKATELLSLMEKTFPELGLKKEDCLEMSWVESMAFSASGFVSAK 360

Query: 361 SLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNE 420
           SLELLL+R+P  NGR KIKSDY  EPISETALEGMWERFK EELE VQ ILIPFGGKMNE
Sbjct: 361 SLELLLNRSPLDNGRQKIKSDYPTEPISETALEGMWERFKNEELEKVQFILIPFGGKMNE 420

Query: 421 ISKAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYR 480
           IS++ETPS +RAGYPIHIGYYV WQ P+ADS+HLEWTREL++YMTPFVSKSPR AY NYR
Sbjct: 421 ISESETPSAHRAGYPIHIGYYVKWQHPDADSRHLEWTRELYNYMTPFVSKSPRAAYANYR 480

Query: 481 DLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRV 540
           DLDIGTNNED I  SYEEAS WGH+YFGNNFERLMEVK KVD FNFFRHEQSIPPAPPRV
Sbjct: 481 DLDIGTNNEDRILTSYEEASVWGHQYFGNNFERLMEVKAKVDQFNFFRHEQSIPPAPPRV 537

Query: 541 Q 542
           +
Sbjct: 541 R 537

BLAST of HG10018256 vs. NCBI nr
Match: KAE8652747.1 (hypothetical protein Csa_013703 [Cucumis sativus])

HSP 1 Score: 932.9 bits (2410), Expect = 1.3e-267
Identity = 459/537 (85.47%), Postives = 489/537 (91.06%), Query Frame = 0

Query: 9   YYYCFISLLLF----PSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSY 68
           Y +C  SLL+      S  TLG G +N++DFVNCIY+HSSNNNDSNSTLIHTPSSSSYSY
Sbjct: 262 YVHCSESLLVTVVVQSSRTTLGCGSNNMKDFVNCIYVHSSNNNDSNSTLIHTPSSSSYSY 321

Query: 69  VLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSYV 128
           VLNFSIRNLRFSEPET KP+AIITPSHASQVQATVICCKSHGLQ+RTRSGGHDFEGRSYV
Sbjct: 322 VLNFSIRNLRFSEPETPKPVAIITPSHASQVQATVICCKSHGLQIRTRSGGHDFEGRSYV 381

Query: 129 ANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVG 188
           ANVPFVLIDL+NLNSITID EDESAWVQSGATVGELY+RIGEKS+TLGFPAGFA TIG+G
Sbjct: 382 ANVPFVLIDLINLNSITIDVEDESAWVQSGATVGELYFRIGEKSRTLGFPAGFAATIGLG 441

Query: 189 GFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVL 248
           GFLSGGG GMMVRKYGL ADNVVDAYVVD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVL
Sbjct: 442 GFLSGGGFGMMVRKYGLGADNVVDAYVVDGNGRVVNRYSMGEDLFWAIRGGGGGSFGIVL 501

Query: 249 AWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGE 308
           AWKL+LV VP IVTSFALH+ WDQNAA LI+ WQYIAP VDQDLFISAWVTASNSS+ G 
Sbjct: 502 AWKLRLVQVPSIVTSFALHKIWDQNAANLIYRWQYIAPWVDQDLFISAWVTASNSSHDGS 561

Query: 309 -RIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLE 368
            RIMEASF SLFLG ATELL+LMEKTFPELGLKKEDCLE SWVE+MAFSASGFVS +SLE
Sbjct: 562 GRIMEASFFSLFLGNATELLSLMEKTFPELGLKKEDCLETSWVESMAFSASGFVSAKSLE 621

Query: 369 LLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISK 428
           LLLDRTP  NGR+K KSDYA EPISET LEGMWERFK EELETVQLILIPFGGK NEIS+
Sbjct: 622 LLLDRTPLHNGRYKTKSDYATEPISETVLEGMWERFKDEELETVQLILIPFGGKTNEISE 681

Query: 429 AETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLD 488
           +ETPSP+RAGYPIHIGYY+TWQRP+ADSKHL+W RELH+YMTPFVSKSPR AYVNYRDLD
Sbjct: 682 SETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVNYRDLD 741

Query: 489 IGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRV 541
           +GTNN+DG+P   EEAS WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP  V
Sbjct: 742 MGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPTSV 798

BLAST of HG10018256 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-139
Identity = 246/514 (47.86%), Postives = 348/514 (67.70%), Query Frame = 0

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETLKPLAIITPSHA 92
           E+F+ C   H   NN +N  L++T     Y  +LN +I+NLRF    T KPL I+TPS+ 
Sbjct: 32  ENFLKCFSKHIP-NNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNN 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +QAT++C K  GLQ+RTRSGGHD EG SY++ VPFV++DL N++SI ID   ++AWV+
Sbjct: 92  SHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY I EK++ L FP G+ PT+GVGG  SGGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T F++ +  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLV 271

Query: 273 KLIHGWQYIAPEVDQDL-FISAWVTASNSSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL + WQ IA + D+DL  ++ ++T + + N G+    +   F S+F G    L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL----ELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC E SW++   F  SG V+  +     E+LLDR+  K     IK DY  
Sbjct: 332 SFPELGIKKTDCKEFSWIDTTIF-YSGVVNFNTANFKKEILLDRSAGKKTAFSIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           +PI ETA+  + E+   E++     +L P+GG M EIS++  P P+RAG    + Y  +W
Sbjct: 392 KPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTASW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KH+ W R ++++ TP+VS++PR+AY+NYRDLD+G  N    P +Y +A  WG 
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNH-ASPNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPP 539
           +YFG NF RL++VK KVDP NFFR+EQSIPP PP
Sbjct: 512 KYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of HG10018256 vs. ExPASy Swiss-Prot
Match: Q9FI25 (Berberine bridge enzyme-like 27 OS=Arabidopsis thaliana OX=3702 GN=At5g44410 PE=2 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 4.4e-138
Identity = 257/542 (47.42%), Postives = 357/542 (65.87%), Query Frame = 0

Query: 1   MEYFWSYYYYYCFISLLL--FPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPS 60
           + +  S + Y+  ++L L  FPS++       N E+F+ C+   S   N+ +S +IHT  
Sbjct: 4   LRFLLSLFIYFLLLNLSLSHFPSISA---QRTNHENFLKCL---SHRINEDDSRIIHTSK 63

Query: 61  SSSYSYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDF 120
             SY  +LN SI+N RF   ET KP++IITP  A+ VQ+T+ C + HG+ +RTRSGGHD+
Sbjct: 64  DPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDY 123

Query: 121 EGRSYVA-NVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGF 180
           EG SY+A + PFV+IDL NL SIT+D ++ + WVQSGAT+GELYY IG+ S++L FPAG 
Sbjct: 124 EGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGL 183

Query: 181 APTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGG 240
            PT+G+GG   GGG G ++RKYGL+ADNV+DA++VDANG  ++R  MGED FWAIRGGGG
Sbjct: 184 YPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGG 243

Query: 241 GSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTAS 300
            SF +VL+WK++L+ VP +VT F + +T ++ A  +I+ WQYIA +V  DLFI A +   
Sbjct: 244 SSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQK- 303

Query: 301 NSSNGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFV 360
                 E  + ASF  L+LG  ++LLALM+  FPELGL+  +C EMSW+E++ +    F+
Sbjct: 304 ------ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FI 363

Query: 361 STESLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGK 420
             ES+E+L  R        K K D+  EPI +TA++ +W RF+  E    ++IL PFGGK
Sbjct: 364 KGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGK 423

Query: 421 MNEISKAETPSPYRAGYPIHIGYYVTWQRPEADS-----KHLEWTRELHDYMTPFVSKSP 480
           M+EI+  E P P+R G    I Y   W   E  +     K+L W   ++++MTP+VSKSP
Sbjct: 424 MSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSP 483

Query: 481 RVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQS 535
           R AYVN+RD+D+G      +   YEEA  WG +YF NNF+RL+ VK  VDP +FF  EQS
Sbjct: 484 RRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQS 527

BLAST of HG10018256 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.8e-137
Identity = 258/534 (48.31%), Postives = 348/534 (65.17%), Query Frame = 0

Query: 13  FISLLLFPSLATL--------GYGGDNLEDFVNCIYLHSSNNNDSN--STLIHTPSSSSY 72
           F S+L+F + +TL             N   F+ C+ L     NDSN  S +IHTP+ +S+
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL---NDSNIVSKVIHTPNDTSF 66

Query: 73  SYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRS 132
           S VL  SI+N RFS P+  KP+ I+TP   S VQ+ V C +  G+ +RTRSGGHD+EG S
Sbjct: 67  SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLS 126

Query: 133 YVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIG 192
           YV + PFV++DL NL SIT+D ++ S WVQ+GAT+GELYY IG+K++TL FPAG  PT+G
Sbjct: 127 YVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVG 186

Query: 193 VGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGI 252
           VGG  SGGG G ++RK+GLAAD+V+DA VVDA GR++ R  MGED FWAIRGGGG SF +
Sbjct: 187 VGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCV 246

Query: 253 VLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNG 312
           VL+WK+ L+ VP  VT F + +  +Q+A K+IH WQ++A +V  DLFI   +        
Sbjct: 247 VLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRY----- 306

Query: 313 GERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL 372
            + ++ ASF  L+LG    LL ++ K FPELGL+++DC EMSW+E++ + A   +  E +
Sbjct: 307 -KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE--LGEEPI 366

Query: 373 ELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEIS 432
            +L  RT       K KSD+  EP+ +TA+  +W R +  E E  QLI  PFGGKM+EI+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 433 KAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDL 492
             ETP P+R G    I Y   W R +   K++ W   ++D M+ FV+KSPR AY+N RDL
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 486

Query: 493 DIGTNNEDGIPIS-YEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPP 536
           D+G     G+  S YEE  +WG +YF NNFERL+ VK  VDP +FF  EQSIPP
Sbjct: 487 DLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of HG10018256 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 488.8 bits (1257), Expect = 8.3e-137
Identity = 240/514 (46.69%), Postives = 344/514 (66.93%), Query Frame = 0

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETLKPLAIITPSHA 92
           E+F+ C +     NN +N   I+T     Y  VLN +I+NLRF+   T KPL I+TPS+ 
Sbjct: 32  ENFLKC-FSEYIPNNPANPKFIYTQHDQLYMSVLNSTIQNLRFTSDTTPKPLVIVTPSNV 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +QA+++C K  GLQ+RTRSGGHD EG SY++ VPF ++DL N++++ +D   ++AWV+
Sbjct: 92  SHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAIVDLRNMHTVKVDIHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY I E ++   FP G+ PT+GVGG  SGGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP   T F++ +  +     
Sbjct: 212 NVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVVVPSKATIFSVKKNMEIHGLV 271

Query: 273 KLIHGWQYIAPEVDQDLFISAWVTASN-SSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL + WQ IA + D+DL ++      N + N G+    +   F S+FLG    L+ LM K
Sbjct: 272 KLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTVHGYFSSIFLGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL----ELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC E+SW++   F  SG V+  +     E+LLDR+  K     IK DY  
Sbjct: 332 SFPELGIKKTDCKELSWIDTTIF-YSGVVNYNTANFKKEILLDRSAGKKTAFSIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           + I ETA+  + E+   EE+     +L P+GG M+EIS++  P P+RAG    + Y  TW
Sbjct: 392 KLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISESAIPFPHRAGIMYELWYTATW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KH+ W R ++++ TP+VS++PR+AY+NYRDLD+G  N +  P +Y +A  WG 
Sbjct: 452 EKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNPES-PNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPP 539
           +YFG NF RL++VK K DP NFFR+EQSIPP PP
Sbjct: 512 KYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLPP 542

BLAST of HG10018256 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 1.4e-136
Identity = 241/513 (46.98%), Postives = 351/513 (68.42%), Query Frame = 0

Query: 33  EDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETLKPLAIITPSHA 92
           E+F+ C +     NN +N  L++T ++  Y  VLN +I NLRF+   T KPL I+TPSH 
Sbjct: 32  ENFLKC-FSQYIPNNATNLKLVYTQNNPLYMSVLNSTIHNLRFTSDTTPKPLVIVTPSHV 91

Query: 93  SQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWVQ 152
           S +Q T++C K  GLQ+RTRSGGHD EG SY++ VPFV++DL N+ SI ID   ++AWV+
Sbjct: 92  SHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDLRNMRSIKIDVHSQTAWVE 151

Query: 153 SGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVV 212
           +GAT+GE+YY + EK++ L   AG+ PT+  GG   GGG G ++R YGLAADN++DA++V
Sbjct: 152 AGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGPLMRNYGLAADNIIDAHLV 211

Query: 213 DANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWD-QNAA 272
           + +G+V++R SMGEDLFWA+RGGG  SFGI++AWK++LV VP   T F++ +  +     
Sbjct: 212 NVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVPK-STMFSVKKIMEIHELV 271

Query: 273 KLIHGWQYIAPEVDQDLFI-SAWVTASNSSNGGER--IMEASFISLFLGKATELLALMEK 332
           KL++ WQ IA + D+DL + + ++T + + N G+    +   F S+FLG    L+ LM K
Sbjct: 272 KLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTYFSSVFLGGVDSLVDLMNK 331

Query: 333 TFPELGLKKEDCLEMSWVEAMAFSASGFVSTE----SLELLLDRTPPKNGRHKIKSDYAI 392
           +FPELG+KK DC ++SW++ + F  SG V+ +    + E+LLDR+  +NG  KIK DY  
Sbjct: 332 SFPELGIKKTDCRQLSWIDTIIF-YSGVVNYDTDNFNKEILLDRSAGQNGAFKIKLDYVK 391

Query: 393 EPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTW 452
           +PI E+    + E+   E++      L P+GG M+EIS++  P P+RAG    + Y  +W
Sbjct: 392 KPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIPFPHRAGILYELWYICSW 451

Query: 453 QRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGH 512
           ++ E + KHL W R ++++MTP+VSK+PR+AY+NYRDLDIG N+    P +Y +A  WG 
Sbjct: 452 EKQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIGINDPKN-PNNYTQARIWGE 511

Query: 513 RYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 538
           +YFG NF+RL++VK  VDP NFFR+EQSIPP P
Sbjct: 512 KYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPLP 540

BLAST of HG10018256 vs. ExPASy TrEMBL
Match: A0A5D3D0K7 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00130 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.2e-265
Identity = 447/510 (87.65%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 32  LEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETLKPLAIITPSH 91
           +EDFV+CIY+HSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPET KP+AIITPSH
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 92  ASQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWV 151
           ASQVQATVICCKSHGLQ+RTRSGGHDFEGRSYVANVPFVLIDL+NLNSITID EDE+AWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 152 QSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYV 211
           QSGATVGELYY+IGEKS+TLGFPAGF  TIG+GGFLSGGG+GMMVRKYGL ADNVVDAYV
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 212 VDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAA 271
           VD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP IVTSFALH+TWDQNAA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 272 KLIHGWQYIAPEVDQDLFISAWVTASNSS-NGGERIMEASFISLFLGKATELLALMEKTF 331
            LI+ WQYIAP VDQDLFISAWVTASNSS +   RIMEASF SLFLG ATELL+LMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 332 PELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEPISET 391
           PELGLKK+DCLEMSWVE+MAFSASGFV  +SLELLLDRTP  NGR+K KSDYA EPIS+T
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360

Query: 392 ALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQRPEAD 451
           AL+GMWERFK EELETVQLILIPFGGKMNEIS++ETPSP+RAGYPIHIGYY+TWQ+P AD
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420

Query: 452 SKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNN 511
           S++LEW RELHDYMTPFVSKSPR AYVNYRDLD+GTNN+DG+P  Y+EAS WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480

Query: 512 FERLMEVKMKVDPFNFFRHEQSIPPAPPRV 541
           FERLMEVKMKVDPFNFFRHEQSIPPAPPRV
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPAPPRV 510

BLAST of HG10018256 vs. ExPASy TrEMBL
Match: A0A1S3CIZ3 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103501494 PE=3 SV=1)

HSP 1 Score: 924.5 bits (2388), Expect = 2.2e-265
Identity = 447/510 (87.65%), Postives = 480/510 (94.12%), Query Frame = 0

Query: 32  LEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETLKPLAIITPSH 91
           +EDFV+CIY+HSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPET KP+AIITPSH
Sbjct: 1   MEDFVDCIYVHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRNLRFSEPETPKPVAIITPSH 60

Query: 92  ASQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLIDLVNLNSITIDTEDESAWV 151
           ASQVQATVICCKSHGLQ+RTRSGGHDFEGRSYVANVPFVLIDL+NLNSITID EDE+AWV
Sbjct: 61  ASQVQATVICCKSHGLQIRTRSGGHDFEGRSYVANVPFVLIDLINLNSITIDVEDETAWV 120

Query: 152 QSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYV 211
           QSGATVGELYY+IGEKS+TLGFPAGF  TIG+GGFLSGGG+GMMVRKYGL ADNVVDAYV
Sbjct: 121 QSGATVGELYYKIGEKSRTLGFPAGFKATIGLGGFLSGGGIGMMVRKYGLGADNVVDAYV 180

Query: 212 VDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAA 271
           VD NGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKL+LV VP IVTSFALH+TWDQNAA
Sbjct: 181 VDRNGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLRLVQVPSIVTSFALHKTWDQNAA 240

Query: 272 KLIHGWQYIAPEVDQDLFISAWVTASNSS-NGGERIMEASFISLFLGKATELLALMEKTF 331
            LI+ WQYIAP VDQDLFISAWVTASNSS +   RIMEASF SLFLG ATELL+LMEKTF
Sbjct: 241 NLIYRWQYIAPWVDQDLFISAWVTASNSSKDESGRIMEASFFSLFLGNATELLSLMEKTF 300

Query: 332 PELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEPISET 391
           PELGLKK+DCLEMSWVE+MAFSASGFV  +SLELLLDRTP  NGR+K KSDYA EPIS+T
Sbjct: 301 PELGLKKKDCLEMSWVESMAFSASGFVYAKSLELLLDRTPVDNGRYKTKSDYATEPISKT 360

Query: 392 ALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQRPEAD 451
           AL+GMWERFK EELETVQLILIPFGGKMNEIS++ETPSP+RAGYPIHIGYY+TWQ+P AD
Sbjct: 361 ALKGMWERFKDEELETVQLILIPFGGKMNEISESETPSPHRAGYPIHIGYYLTWQQPNAD 420

Query: 452 SKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNN 511
           S++LEW RELHDYMTPFVSKSPR AYVNYRDLD+GTNN+DG+P  Y+EAS WGHRYFGNN
Sbjct: 421 SRNLEWARELHDYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNN 480

Query: 512 FERLMEVKMKVDPFNFFRHEQSIPPAPPRV 541
           FERLMEVKMKVDPFNFFRHEQSIPPAPPRV
Sbjct: 481 FERLMEVKMKVDPFNFFRHEQSIPPAPPRV 510

BLAST of HG10018256 vs. ExPASy TrEMBL
Match: A0A6J1I038 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469264 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 424/551 (76.95%), Postives = 486/551 (88.20%), Query Frame = 0

Query: 13  FISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRN 72
           F+S L+F S A+ G G DNL+DF++CI LHSSN+N SNSTLIHTPSSSSY YVLNFSIRN
Sbjct: 17  FVS-LVFVSFASWGCGSDNLQDFLHCISLHSSNHN-SNSTLIHTPSSSSYLYVLNFSIRN 76

Query: 73  LRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLI 132
           LRF+E +T KP+AI+TP  ASQVQATVICCK+HGLQ+RTRSGG DFEGRSYV+NVPFVLI
Sbjct: 77  LRFAESDTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFVLI 136

Query: 133 DLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGL 192
           DL+NLNSITIDTEDE+AW QSGATVGELYY+IG+KS+TL FPAG    +G+GGF SGGG 
Sbjct: 137 DLINLNSITIDTEDETAWAQSGATVGELYYKIGQKSRTLAFPAGLVANLGLGGFFSGGGF 196

Query: 193 GMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 252
           GM++RKYGLA DNVVDA +VDANG++++R SMGEDLFWAIRGGGGGSFGIVL WKLKLV 
Sbjct: 197 GMLLRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKLVR 256

Query: 253 VPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFI 312
           VPP VTSFA+H++WD NAAKLIH WQY+APEVD+DLFI+AWVTASNSS+GG RIM+ASF 
Sbjct: 257 VPPTVTSFAVHKSWDLNAAKLIHQWQYVAPEVDEDLFITAWVTASNSSDGGGRIMKASFF 316

Query: 313 SLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPP- 372
           +LFLG+A EL++LM K FPEL L +EDCLEMSWVE+MAFSAS FVS E+LELLLDRT   
Sbjct: 317 ALFLGEAAELVSLMAKAFPELELTREDCLEMSWVESMAFSASVFVSAENLELLLDRTTQL 376

Query: 373 KNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYR 432
            NGR+KIKSDYA EPISETAL G+WERFKIEELET+QLILIPFGGKMNEIS+ +TP+P+R
Sbjct: 377 SNGRYKIKSDYATEPISETALNGIWERFKIEELETLQLILIPFGGKMNEISETDTPNPHR 436

Query: 433 AGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDG 492
           A +PIHIGYY TW RP+ADS+HLEWTRELHDYMTPFVSKSPR AY+N +DLDIG NNEDG
Sbjct: 437 ARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNEDG 496

Query: 493 IPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRVQGIKAKMGSCL 552
           IP SYEEAS WGH+YFG NFERL+EVK K DPFNFFRHEQSIPP  P ++GI+AKM SC 
Sbjct: 497 IPTSYEEASIWGHQYFGINFERLVEVKTKADPFNFFRHEQSIPPVAP-IRGIQAKMASCS 556

Query: 553 DVPYETMSCAI 563
           D+ YETM  A+
Sbjct: 557 DMSYETMPSAL 564

BLAST of HG10018256 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.9e-249
Identity = 426/540 (78.89%), Postives = 482/540 (89.26%), Query Frame = 0

Query: 13  FISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSIRN 72
           FISLLL  S A+ G+G DNLEDF++CI LHSSN + SNSTLIHTPSSSSY YVLNFSIRN
Sbjct: 26  FISLLLL-SFASWGFGSDNLEDFLHCISLHSSNRH-SNSTLIHTPSSSSYPYVLNFSIRN 85

Query: 73  LRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFVLI 132
           LRF+E  T KP+AI+TP  ASQVQATVICCK+HGLQ+RTRSGGHDFEGRSYV+N+PFVLI
Sbjct: 86  LRFAEYHTPKPVAIVTPWQASQVQATVICCKTHGLQIRTRSGGHDFEGRSYVSNIPFVLI 145

Query: 133 DLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGL 192
           DL+NLNSITID +DE+AW QSGATVGELYY+IGEKS+TL FPAG   +IG+GGF SGGGL
Sbjct: 146 DLINLNSITIDVDDETAWAQSGATVGELYYKIGEKSRTLAFPAGITASIGLGGFFSGGGL 205

Query: 193 GMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVP 252
           GM+VRKYGLA DNVVDAY+VDANG++++R SMGEDLFWAIRGGGGGSFGIVLAWKLKLV 
Sbjct: 206 GMLVRKYGLAVDNVVDAYLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLAWKLKLVR 265

Query: 253 VPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFI 312
           VPP VTSFA+H++WD NAAKLIH WQY+A EVD+DLFI+A VTASNSS  G RIM+ASF 
Sbjct: 266 VPPTVTSFAVHKSWDLNAAKLIHWWQYVALEVDEDLFITARVTASNSSKEGGRIMKASFF 325

Query: 313 SLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPK 372
           SLFLGKA EL++LMEK FPEL LKKEDCLE+SWVEAMAFSAS +VS E+LELLLDRT   
Sbjct: 326 SLFLGKAAELVSLMEKAFPELELKKEDCLEISWVEAMAFSASEYVSPENLELLLDRTSLI 385

Query: 373 NGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRA 432
            GR+K+KSDYA +PISETAL G+WERFKIEELETVQLILIPFGGKMNEIS+ +TP+P+RA
Sbjct: 386 TGRYKMKSDYATKPISETALNGIWERFKIEELETVQLILIPFGGKMNEISETDTPNPHRA 445

Query: 433 GYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGI 492
            +PIHIGYYVTW RP+ADS+HLEWTRELHDYMTPFVS+SPR AY+NY+DLDIG NNEDGI
Sbjct: 446 SHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYMNYKDLDIGKNNEDGI 505

Query: 493 PISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRVQGIKAKMGSCLD 552
           P SY+EAS WG+RYFG NFERL+EVK KVDPFNFFRHEQSIPPA P + GI+AKM SC D
Sbjct: 506 PTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAAP-MGGIQAKMASCPD 562

BLAST of HG10018256 vs. ExPASy TrEMBL
Match: A0A6J1EVN7 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111438506 PE=3 SV=1)

HSP 1 Score: 870.9 bits (2249), Expect = 2.9e-249
Identity = 425/553 (76.85%), Postives = 481/553 (86.98%), Query Frame = 0

Query: 11  YCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYSYVLNFSI 70
           + FIS L+F S A+ G G DNL+DF++CI LHSSN+N SNSTLIHTPSSSSY YVLNFSI
Sbjct: 15  FSFIS-LVFVSFASWGCGSDNLQDFLHCISLHSSNHN-SNSTLIHTPSSSSYLYVLNFSI 74

Query: 71  RNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANVPFV 130
           RNLRF+E +T KP+AI+TP  AS+VQ TVICCK+HGLQ+RTRSGG DFEGRSYV+NVPFV
Sbjct: 75  RNLRFAESDTPKPVAIVTPWQASEVQTTVICCKTHGLQIRTRSGGQDFEGRSYVSNVPFV 134

Query: 131 LIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGG 190
           LIDL NLNSITIDTEDE+AW QSGATVGELYY+IGEKS+TL FPAG    +G+GGF SGG
Sbjct: 135 LIDLTNLNSITIDTEDETAWAQSGATVGELYYKIGEKSRTLAFPAGLVANLGLGGFFSGG 194

Query: 191 GLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKL 250
           G GM+VRKYGLA DNVVDA +VDANG++++R SMGEDLFWAIRGGGGGSFGIVL WKLKL
Sbjct: 195 GFGMLVRKYGLAVDNVVDAQLVDANGKLLDRDSMGEDLFWAIRGGGGGSFGIVLTWKLKL 254

Query: 251 VPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEAS 310
           V VPP VTSFA+H++WD NAAKLIH WQY+APE D+DLFI+AWV ASNSS+GG RIM+AS
Sbjct: 255 VRVPPTVTSFAVHKSWDLNAAKLIHRWQYVAPEADEDLFITAWVIASNSSDGGGRIMKAS 314

Query: 311 FISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTP 370
           F SLFLG+A EL++LM K FPEL L KEDCLEMSWVE+MAFSAS FVS ESLELLLDRT 
Sbjct: 315 FFSLFLGEAAELVSLMAKAFPELELTKEDCLEMSWVESMAFSASVFVSAESLELLLDRTT 374

Query: 371 P-KNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSP 430
              NGR+KIKSDYA EPISETAL G+WERFKIEELET+QL+LIPFGGKMNEIS+ +TP+P
Sbjct: 375 QLSNGRYKIKSDYATEPISETALNGIWERFKIEELETLQLVLIPFGGKMNEISETDTPNP 434

Query: 431 YRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNE 490
           +RA +PIHIGYY TW RP+ADS+HLEWTRELHDYMTPFVSKSPR AY+N +DLDIG NNE
Sbjct: 435 HRARHPIHIGYYATWHRPDADSRHLEWTRELHDYMTPFVSKSPRAAYINNKDLDIGRNNE 494

Query: 491 DGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAPPRVQGIKAKMGS 550
           DGIP SYEEAS WGH+YFG NFERL+ VK K DPFNFFRHEQSIPP  P + GI+AKM S
Sbjct: 495 DGIPTSYEEASIWGHQYFGINFERLVAVKTKADPFNFFRHEQSIPPVAP-IGGIQAKMAS 554

Query: 551 CLDVPYETMSCAI 563
           C DV YETM  A+
Sbjct: 555 CSDVSYETMPSAM 564

BLAST of HG10018256 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 493.0 bits (1268), Expect = 3.1e-139
Identity = 257/542 (47.42%), Postives = 357/542 (65.87%), Query Frame = 0

Query: 1   MEYFWSYYYYYCFISLLL--FPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPS 60
           + +  S + Y+  ++L L  FPS++       N E+F+ C+   S   N+ +S +IHT  
Sbjct: 4   LRFLLSLFIYFLLLNLSLSHFPSISA---QRTNHENFLKCL---SHRINEDDSRIIHTSK 63

Query: 61  SSSYSYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDF 120
             SY  +LN SI+N RF   ET KP++IITP  A+ VQ+T+ C + HG+ +RTRSGGHD+
Sbjct: 64  DPSYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDY 123

Query: 121 EGRSYVA-NVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGF 180
           EG SY+A + PFV+IDL NL SIT+D ++ + WVQSGAT+GELYY IG+ S++L FPAG 
Sbjct: 124 EGLSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGL 183

Query: 181 APTIGVGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGG 240
            PT+G+GG   GGG G ++RKYGL+ADNV+DA++VDANG  ++R  MGED FWAIRGGGG
Sbjct: 184 YPTVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGG 243

Query: 241 GSFGIVLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTAS 300
            SF +VL+WK++L+ VP +VT F + +T ++ A  +I+ WQYIA +V  DLFI A +   
Sbjct: 244 SSFSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAMLQK- 303

Query: 301 NSSNGGERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFV 360
                 E  + ASF  L+LG  ++LLALM+  FPELGL+  +C EMSW+E++ +    F+
Sbjct: 304 ------ETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLW----FI 363

Query: 361 STESLELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGK 420
             ES+E+L  R        K K D+  EPI +TA++ +W RF+  E    ++IL PFGGK
Sbjct: 364 KGESMEILAKRKRTSRS-FKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGK 423

Query: 421 MNEISKAETPSPYRAGYPIHIGYYVTWQRPEADS-----KHLEWTRELHDYMTPFVSKSP 480
           M+EI+  E P P+R G    I Y   W   E  +     K+L W   ++++MTP+VSKSP
Sbjct: 424 MSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSP 483

Query: 481 RVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQS 535
           R AYVN+RD+D+G      +   YEEA  WG +YF NNF+RL+ VK  VDP +FF  EQS
Sbjct: 484 RRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQS 527

BLAST of HG10018256 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 489.6 bits (1259), Expect = 3.4e-138
Identity = 258/534 (48.31%), Postives = 348/534 (65.17%), Query Frame = 0

Query: 13  FISLLLFPSLATL--------GYGGDNLEDFVNCIYLHSSNNNDSN--STLIHTPSSSSY 72
           F S+L+F + +TL             N   F+ C+ L     NDSN  S +IHTP+ +S+
Sbjct: 7   FSSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCLSLQL---NDSNIVSKVIHTPNDTSF 66

Query: 73  SYVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRS 132
           S VL  SI+N RFS P+  KP+ I+TP   S VQ+ V C +  G+ +RTRSGGHD+EG S
Sbjct: 67  SSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLS 126

Query: 133 YVANVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIG 192
           YV + PFV++DL NL SIT+D ++ S WVQ+GAT+GELYY IG+K++TL FPAG  PT+G
Sbjct: 127 YVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVG 186

Query: 193 VGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGI 252
           VGG  SGGG G ++RK+GLAAD+V+DA VVDA GR++ R  MGED FWAIRGGGG SF +
Sbjct: 187 VGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCV 246

Query: 253 VLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNG 312
           VL+WK+ L+ VP  VT F + +  +Q+A K+IH WQ++A +V  DLFI   +        
Sbjct: 247 VLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVMLQRY----- 306

Query: 313 GERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL 372
            + ++ ASF  L+LG    LL ++ K FPELGL+++DC EMSW+E++ + A   +  E +
Sbjct: 307 -KNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAE--LGEEPI 366

Query: 373 ELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEIS 432
            +L  RT       K KSD+  EP+ +TA+  +W R +  E E  QLI  PFGGKM+EI+
Sbjct: 367 NVLTKRTRASLA-FKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMSEIA 426

Query: 433 KAETPSPYRAGYPIHIGYYVTWQRPEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDL 492
             ETP P+R G    I Y   W R +   K++ W   ++D M+ FV+KSPR AY+N RDL
Sbjct: 427 DYETPFPHRKGNIYEIQYLNYW-RGDVKEKYMRWVERVYDDMSEFVAKSPRGAYINLRDL 486

Query: 493 DIGTNNEDGIPIS-YEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPP 536
           D+G     G+  S YEE  +WG +YF NNFERL+ VK  VDP +FF  EQSIPP
Sbjct: 487 DLGM--YVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIPP 525

BLAST of HG10018256 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 482.6 bits (1241), Expect = 4.2e-136
Identity = 254/537 (47.30%), Postives = 350/537 (65.18%), Query Frame = 0

Query: 4   FWSYYYYYCFISLLLFPSLATLGYGGDNLEDFVNCIYLHSSNNNDSNSTLIHTPSSSSYS 63
           F S+ +     SL + P ++     G N EDF+ C+  +  N+N     +IHT   SS+ 
Sbjct: 3   FSSFLFTILLFSLNISPLVSA---HGSNHEDFLKCL-SYRMNDNTVEPKVIHTSKDSSFF 62

Query: 64  YVLNFSIRNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSY 123
            +L+ SI+N RFS  ET KP++IITP  AS VQ  + C + HG+ +RTRS GH +EG SY
Sbjct: 63  SILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSY 122

Query: 124 VA-NVPFVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIG 183
           +A N PF +IDL NL SI++D ++ + WVQ+GAT GELYY IG+ +++L FPAG  PT+G
Sbjct: 123 IAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVG 182

Query: 184 VGGFLSGGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGI 243
           VGG  SGGG G ++RKYGLAADN++DA VVDA+GR+++R +MGED FWAIRGGGG SFG+
Sbjct: 183 VGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGV 242

Query: 244 VLAWKLKLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNG 303
           +L+WK+KLV VP  +T F + +T  + A ++I  WQY A +V  DLFI   +  SN    
Sbjct: 243 ILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSN---- 302

Query: 304 GERIMEASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESL 363
            +  + A F  L++G    LLALME+ FPELGL+KE C EMSW+E++ + A  F   ESL
Sbjct: 303 -KNAVHALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFAD-FPKGESL 362

Query: 364 ELLLDRTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEIS 423
            +L +R        K K D+  EPI E A++ +W R +  E    ++IL PFGGKM+E++
Sbjct: 363 GVLTNRERTSLS-FKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMA 422

Query: 424 KAETPSPYRAGYPIHIGYYVTWQRPE-----ADSKHLEWTRELHDYMTPFVSKSPRVAYV 483
           + ETP P+R G    I Y   W+  E        K+L+W   ++++MTP+VSKSPR AYV
Sbjct: 423 EYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYV 482

Query: 484 NYRDLDIGTNNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           N++D+D+G          YEE  +WG +YF NNFERL+ VK +VDP +FF  EQSIP
Sbjct: 483 NFKDMDLGMYLGKK-KTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of HG10018256 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 480.3 bits (1235), Expect = 2.1e-135
Identity = 251/528 (47.54%), Postives = 346/528 (65.53%), Query Frame = 0

Query: 13  FISLLLFPSLATLGYGGDNL-EDFVNCIYLHSSNNNDSNSTLI-HTPSSSSYSYVLNFSI 72
           F+  LL  SL+ +      + +DFV C+  +S  +   +S+   H  ++SS+   L  S 
Sbjct: 12  FLVTLLLLSLSYIPLSFSTIQQDFVMCLVDNSDASFPMDSSFFTHDLNASSFKLALETSA 71

Query: 73  RNLRFSEPETLKPLAIITPSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSYVANV--P 132
           +NLR+  P   KP  I  P + + VQA V+C K   L LR RSGGHD+EG SYV+ +   
Sbjct: 72  QNLRYLMPSNPKPEFIFEPLYETHVQAAVLCAKKLKLHLRLRSGGHDYEGLSYVSEMETA 131

Query: 133 FVLIDLVNLNSITIDTEDESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLS 192
           FV++DL  L  I++D E  SAWV +GA++GE+YYRI EKS+  GFPAG   ++G+GG + 
Sbjct: 132 FVIVDLSKLRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHII 191

Query: 193 GGGLGMMVRKYGLAADNVVDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKL 252
           GG  G M+RK+GL ADNV+DA +VDA+G+++NR +MGED+FWAIRGGGGGSFG++LAWK+
Sbjct: 192 GGAYGSMMRKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKI 251

Query: 253 KLVPVPPIVTSFALHRTWDQNAAKLIHGWQYIAPEVDQDLFISAWV-TASNSSNGGERIM 312
           KLVPVP IVT F + RT +Q+  KL++ WQ +A ++D+DLFI   +   S +    ER +
Sbjct: 252 KLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQPTSKTPKSKERTI 311

Query: 313 EASFISLFLGKATELLALMEKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLD 372
             S+   FLG A  LL +M+++FP+LGL K+DCLE SW++++ + A GF ST   E LLD
Sbjct: 312 STSYQGQFLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIA-GFPSTAPSEALLD 371

Query: 373 RTPPKNGRHKIKSDYAIEPISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETP 432
                    K KSDY  EPI    LEG+WE  K+ E ++   I  P+GG M +I + ETP
Sbjct: 372 GKSLFKNYFKAKSDYVEEPIPVEGLEGLWE--KLLEEDSPLTIWNPYGGMMAKIPETETP 431

Query: 433 SPYRAGYPIHIGYYVTWQRPE-ADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGT 492
            P+R+G    I +   WQ  + +++KH+ W RE++ YM  +VSKSPR AYVNYRDLD+G 
Sbjct: 432 FPHRSGTLFKIQWLTLWQDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGM 491

Query: 493 NNEDGIPISYEEASAWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIP 535
           N +        +A  WG+RYF  NFERL+E+K K DP NFFRHEQSIP
Sbjct: 492 NGKG------SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIP 530

BLAST of HG10018256 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 479.9 bits (1234), Expect = 2.7e-135
Identity = 243/511 (47.55%), Postives = 337/511 (65.95%), Query Frame = 0

Query: 31  NLEDFVNCIYLHSSNNNDSN--STLIHTPSSSSYSYVLNFSIRNLRFSEPETLKPLAIIT 90
           N E F  C+   +SN++  +  S  I    + SYS VL  +IRNLRF+   T KP  II 
Sbjct: 24  NSETFTQCL---TSNSDPKHPISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIA 83

Query: 91  PSHASQVQATVICCKSHGLQLRTRSGGHDFEGRSYV--ANVPFVLIDLVNLNSITIDTED 150
            +H S VQA + C K H LQ++ RSGGHD++G SYV  +  PF ++D+ NL S+ +D   
Sbjct: 84  ATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVAS 143

Query: 151 ESAWVQSGATVGELYYRIGEKSQTLGFPAGFAPTIGVGGFLSGGGLGMMVRKYGLAADNV 210
           ++AWVQ+GA +GE+YY I EKS+TL +PAG  PT+GVGG +SGGG G M+RKYGL  DN 
Sbjct: 144 KTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNT 203

Query: 211 VDAYVVDANGRVVNRYSMGEDLFWAIRGGGGGSFGIVLAWKLKLVPVPPIVTSFALHRTW 270
           +DA +VD NG++++R  MGEDL+WAI GGGGGS+G+VLA+K+ LV VP  VT F + RT 
Sbjct: 204 IDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTL 263

Query: 271 DQNAAKLIHGWQYIAPEVDQDLFISAWVTASNSSNGGERIMEASFISLFLGKATELLALM 330
           +QNA  +IH WQ +AP++  +LFI   +   N +   ++ +  +FI++FLG  T LL+++
Sbjct: 264 EQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQKTVRTTFIAMFLGDTTTLLSIL 323

Query: 331 EKTFPELGLKKEDCLEMSWVEAMAFSASGFVSTESLELLLDRTPPKNGRHKIKSDYAIEP 390
            + FPELGL + DC E SW++++ F  +  V + S  LLL R  P N   K KSDY  EP
Sbjct: 324 NRRFPELGLVRSDCTETSWIQSVLFWTNIQVGS-SETLLLQRNQPVN-YLKRKSDYVREP 383

Query: 391 ISETALEGMWERFKIEELETVQLILIPFGGKMNEISKAETPSPYRAGYPIHIGYYVTWQR 450
           IS T LE +W+  K+ ELE   +   P+GG+M  IS   TP PYRAG    I Y   W+ 
Sbjct: 384 ISRTGLESIWK--KMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRD 443

Query: 451 PEADSKHLEWTRELHDYMTPFVSKSPRVAYVNYRDLDIGTNNEDGIPISYEEASAWGHRY 510
                +++E TR+L+ +MTPFVSK+PR ++ NYRD+D+G N+ +G   SY E   +G +Y
Sbjct: 444 ETLTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKY 503

Query: 511 FGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 538
           F  NFERL+++K +VD  NFFR+EQSIP  P
Sbjct: 504 FAGNFERLVKIKTRVDSGNFFRNEQSIPVLP 527