HG10018250 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10018250
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionMajor facilitator superfamily protein
LocationChr04: 2340606 .. 2342357 (+)
RNA-Seq ExpressionHG10018250
SyntenyHG10018250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTACAACGACCGGAGCCGACGGCGCCCACGGCTGCGCAGCAATCCGATTCCCCTTGCAGGTGATTAAAGGGCGGTGGTTTACCGTTTTCGCTACATTTCTGATCATGGCCGGCGCCGGTGCCACGTATCTGTTCGGTGTCTACTCCAAACAGATCAAAAGGACGCTTGGATATGACCAAACCACTTTGAATCTGATGGGTTTTTCTAAAGATCTGGGTGCTAACGTCGGTGTGCTGTCCGGTTTAGTGGCGGAGGTGATGCCGACTTGGTTCGTTCTCCTCCTTGGCTCCGCCTTGAACTTCACTGGATACTTCATGATTTGGCTTGCGGTTACCGGCAGAATCGCAAAGCCTCAAGTCTGGCAGATGTGTCTTTACATTTGCGTTGGAGCGAATTCCCAGAATTTCGCTAATACTGGCGCTTTGGTTACTTGTATCCTGAATTTCCCCGAGAGCCGTGGGGCTATGATGGGGCTTTTGAAGGGATTCACAGGGCTGAGCGGCGCCATTCTCACACAGATTTATTTGGCCGTCTATGGCGATGATGCCACGTCGTTGATCCTTTTAATTGGGTGGCTCCCGGCGGCGCTTTCCGTCCTTTTTGTTTTCACGATTCGGAGGCTCAGAGTAGAGAGACAACCGAACGAGAAGAGGGTTTTTTACCATTTCCTTTATGTCTCAATTGGGTTAGCGCTTTTCATCATGATTATGAATATTGTTCAGAAGAGAGTGGAATTCAACCATACGGCTTATGCTATAAGTGCTACTGTGATTTGCGTATTTCTTTTCCTTCCTCTGTTTATTGTGATCCGAGAAGAGCTTCATCTCTGGAATGCAAAGAAACTCCCCCCAATGCCTAACGAAAACCCACAATCGCAATCTCAACCAAAATCAATTGATGAACCCAAAATCATCACAGAAGCATCAAAAGAAATCAAGGAAATCGCAAACCCACCACCAGCGTCCTGTTTTTCCAACATTCATCACAAGCCAGCCAGAGGAGACGATTACACAATCCTCCAAGCACTTCTCAGCATCGATATGTTGGTTCTGTTCATCGCAACATTCTGTGGGCTAGGCACAAGCTTAACCGCAGTAGATAATTTGGGGCAAATCGGTGAATCACTCGGCTACCCATTAAAAACAGTAAGCTCATTCGTTTCATTAGTAAGCATTTGGAATTACTTCGGCAGAGTATTCTCTGGATTCGTCTCTGAAACCCTTCTGGCAAAATTCAAATTCCCAAGACCTTTAATGATGACATTAGTCCTTCTTCTCTCTTGCATTGGCCAACTCTTAATCGCTTTCCCAGTGCCAGGCTCCGTCTACATAGCCTCCATCATCATCGGATTCTCGTTCGGCGCACAGCTTCCCCTGCTTTTCGCCATAATTTCAGAGCTATTCGGACTGAAATATTTCTCCACTCTGTTCAATTTTGGGCAAATTGCAAGCCCGTTAGGATCGTATATTCTGAATGTGAAAGTGGCTGGAATAATGTATGACATGGAAGCTTTAAAGCAGCTGAAGGAGAAGGGGTTAGACAGATCGGCGGTGAAGGAATTGACTTGCATTGGAAAGCAATGTTACAGGAAATCGTTTACATTAATGGCGATTGTTACTTTTGTTGGAGCTATGGTTTCGCTGGTTTTAGTGATGAGAACAAGGGAGTTTTACAAAGGGGATATTTACAAGAAGTTCAGAGACGACGAAGTGAAGGAGGAGACCGTTACTGCTCGGGAATCCAAGTGA

mRNA sequence

ATGGGTACAACGACCGGAGCCGACGGCGCCCACGGCTGCGCAGCAATCCGATTCCCCTTGCAGGTGATTAAAGGGCGGTGGTTTACCGTTTTCGCTACATTTCTGATCATGGCCGGCGCCGGTGCCACGTATCTGTTCGGTGTCTACTCCAAACAGATCAAAAGGACGCTTGGATATGACCAAACCACTTTGAATCTGATGGGTTTTTCTAAAGATCTGGGTGCTAACGTCGGTGTGCTGTCCGGTTTAGTGGCGGAGGTGATGCCGACTTGGTTCGTTCTCCTCCTTGGCTCCGCCTTGAACTTCACTGGATACTTCATGATTTGGCTTGCGGTTACCGGCAGAATCGCAAAGCCTCAAGTCTGGCAGATGTGTCTTTACATTTGCGTTGGAGCGAATTCCCAGAATTTCGCTAATACTGGCGCTTTGGTTACTTGTATCCTGAATTTCCCCGAGAGCCGTGGGGCTATGATGGGGCTTTTGAAGGGATTCACAGGGCTGAGCGGCGCCATTCTCACACAGATTTATTTGGCCGTCTATGGCGATGATGCCACGTCGTTGATCCTTTTAATTGGGTGGCTCCCGGCGGCGCTTTCCGTCCTTTTTGTTTTCACGATTCGGAGGCTCAGAGTAGAGAGACAACCGAACGAGAAGAGGGTTTTTTACCATTTCCTTTATGTCTCAATTGGGTTAGCGCTTTTCATCATGATTATGAATATTGTTCAGAAGAGAGTGGAATTCAACCATACGGCTTATGCTATAAGTGCTACTGTGATTTGCGTATTTCTTTTCCTTCCTCTGTTTATTGTGATCCGAGAAGAGCTTCATCTCTGGAATGCAAAGAAACTCCCCCCAATGCCTAACGAAAACCCACAATCGCAATCTCAACCAAAATCAATTGATGAACCCAAAATCATCACAGAAGCATCAAAAGAAATCAAGGAAATCGCAAACCCACCACCAGCGTCCTGTTTTTCCAACATTCATCACAAGCCAGCCAGAGGAGACGATTACACAATCCTCCAAGCACTTCTCAGCATCGATATGTTGGTTCTGTTCATCGCAACATTCTGTGGGCTAGGCACAAGCTTAACCGCAGTAGATAATTTGGGGCAAATCGGTGAATCACTCGGCTACCCATTAAAAACAGTAAGCTCATTCGTTTCATTAGTAAGCATTTGGAATTACTTCGGCAGAGTATTCTCTGGATTCGTCTCTGAAACCCTTCTGGCAAAATTCAAATTCCCAAGACCTTTAATGATGACATTAGTCCTTCTTCTCTCTTGCATTGGCCAACTCTTAATCGCTTTCCCAGTGCCAGGCTCCGTCTACATAGCCTCCATCATCATCGGATTCTCGTTCGGCGCACAGCTTCCCCTGCTTTTCGCCATAATTTCAGAGCTATTCGGACTGAAATATTTCTCCACTCTGTTCAATTTTGGGCAAATTGCAAGCCCGTTAGGATCGTATATTCTGAATGTGAAAGTGGCTGGAATAATGTATGACATGGAAGCTTTAAAGCAGCTGAAGGAGAAGGGGTTAGACAGATCGGCGGTGAAGGAATTGACTTGCATTGGAAAGCAATGTTACAGGAAATCGTTTACATTAATGGCGATTGTTACTTTTGTTGGAGCTATGGTTTCGCTGGTTTTAGTGATGAGAACAAGGGAGTTTTACAAAGGGGATATTTACAAGAAGTTCAGAGACGACGAAGTGAAGGAGGAGACCGTTACTGCTCGGGAATCCAAGTGA

Coding sequence (CDS)

ATGGGTACAACGACCGGAGCCGACGGCGCCCACGGCTGCGCAGCAATCCGATTCCCCTTGCAGGTGATTAAAGGGCGGTGGTTTACCGTTTTCGCTACATTTCTGATCATGGCCGGCGCCGGTGCCACGTATCTGTTCGGTGTCTACTCCAAACAGATCAAAAGGACGCTTGGATATGACCAAACCACTTTGAATCTGATGGGTTTTTCTAAAGATCTGGGTGCTAACGTCGGTGTGCTGTCCGGTTTAGTGGCGGAGGTGATGCCGACTTGGTTCGTTCTCCTCCTTGGCTCCGCCTTGAACTTCACTGGATACTTCATGATTTGGCTTGCGGTTACCGGCAGAATCGCAAAGCCTCAAGTCTGGCAGATGTGTCTTTACATTTGCGTTGGAGCGAATTCCCAGAATTTCGCTAATACTGGCGCTTTGGTTACTTGTATCCTGAATTTCCCCGAGAGCCGTGGGGCTATGATGGGGCTTTTGAAGGGATTCACAGGGCTGAGCGGCGCCATTCTCACACAGATTTATTTGGCCGTCTATGGCGATGATGCCACGTCGTTGATCCTTTTAATTGGGTGGCTCCCGGCGGCGCTTTCCGTCCTTTTTGTTTTCACGATTCGGAGGCTCAGAGTAGAGAGACAACCGAACGAGAAGAGGGTTTTTTACCATTTCCTTTATGTCTCAATTGGGTTAGCGCTTTTCATCATGATTATGAATATTGTTCAGAAGAGAGTGGAATTCAACCATACGGCTTATGCTATAAGTGCTACTGTGATTTGCGTATTTCTTTTCCTTCCTCTGTTTATTGTGATCCGAGAAGAGCTTCATCTCTGGAATGCAAAGAAACTCCCCCCAATGCCTAACGAAAACCCACAATCGCAATCTCAACCAAAATCAATTGATGAACCCAAAATCATCACAGAAGCATCAAAAGAAATCAAGGAAATCGCAAACCCACCACCAGCGTCCTGTTTTTCCAACATTCATCACAAGCCAGCCAGAGGAGACGATTACACAATCCTCCAAGCACTTCTCAGCATCGATATGTTGGTTCTGTTCATCGCAACATTCTGTGGGCTAGGCACAAGCTTAACCGCAGTAGATAATTTGGGGCAAATCGGTGAATCACTCGGCTACCCATTAAAAACAGTAAGCTCATTCGTTTCATTAGTAAGCATTTGGAATTACTTCGGCAGAGTATTCTCTGGATTCGTCTCTGAAACCCTTCTGGCAAAATTCAAATTCCCAAGACCTTTAATGATGACATTAGTCCTTCTTCTCTCTTGCATTGGCCAACTCTTAATCGCTTTCCCAGTGCCAGGCTCCGTCTACATAGCCTCCATCATCATCGGATTCTCGTTCGGCGCACAGCTTCCCCTGCTTTTCGCCATAATTTCAGAGCTATTCGGACTGAAATATTTCTCCACTCTGTTCAATTTTGGGCAAATTGCAAGCCCGTTAGGATCGTATATTCTGAATGTGAAAGTGGCTGGAATAATGTATGACATGGAAGCTTTAAAGCAGCTGAAGGAGAAGGGGTTAGACAGATCGGCGGTGAAGGAATTGACTTGCATTGGAAAGCAATGTTACAGGAAATCGTTTACATTAATGGCGATTGTTACTTTTGTTGGAGCTATGGTTTCGCTGGTTTTAGTGATGAGAACAAGGGAGTTTTACAAAGGGGATATTTACAAGAAGTTCAGAGACGACGAAGTGAAGGAGGAGACCGTTACTGCTCGGGAATCCAAGTGA

Protein sequence

MGTTTGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKRVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEPKIITEASKEIKEIANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEETVTARESK
Homology
BLAST of HG10018250 vs. NCBI nr
Match: XP_038895725.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1043.5 bits (2697), Expect = 6.8e-301
Identity = 542/578 (93.77%), Postives = 558/578 (96.54%), Query Frame = 0

Query: 1   MGTTTGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYD 60
           MGTTTGADGAHGCAA+RF LQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIK TLGYD
Sbjct: 1   MGTTTGADGAHGCAALRFTLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKATLGYD 60

Query: 61  QTTLNLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQ 120
           QTTLNLMGFSKDLGANVGVLSGLVAEV PTWFVLLLGSALNF GYFMIWLAVTGRIAKPQ
Sbjct: 61  QTTLNLMGFSKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFAGYFMIWLAVTGRIAKPQ 120

Query: 121 VWQMCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVY 180
           VWQMCLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGA+LTQIYLAVY
Sbjct: 121 VWQMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAVLTQIYLAVY 180

Query: 181 GDDATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNI 240
           GDDAT+LILLIGWLPAALSV+FVFTIRRLR ERQPNEKRVFYHFLYVSIGLALFIMIMNI
Sbjct: 181 GDDATALILLIGWLPAALSVVFVFTIRRLRAERQPNEKRVFYHFLYVSIGLALFIMIMNI 240

Query: 241 VQKRVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKK--LPPMPNENP--QSQSQ 300
           VQK+VEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKK   PP+PNENP  QSQSQ
Sbjct: 241 VQKKVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKTPTPPIPNENPQSQSQSQ 300

Query: 301 PKSIDEPKIITEASKEIKEIANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIAT 360
           PK +DE KIITE S +IKEI NPPP SCFSNI HKPARGDDYTILQALLS+DMLVLFIAT
Sbjct: 301 PKPVDESKIITEESNQIKEIPNPPPDSCFSNICHKPARGDDYTILQALLSVDMLVLFIAT 360

Query: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFP 420
           FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFP
Sbjct: 361 FCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFP 420

Query: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFST 480
           RPLMMTLVLLLSCIGQLLIAFPVPGSVY+AS+IIGFSFGAQLPLLFAIISELFGLKYFST
Sbjct: 421 RPLMMTLVLLLSCIGQLLIAFPVPGSVYLASVIIGFSFGAQLPLLFAIISELFGLKYFST 480

Query: 481 LFNFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTL 540
           LFN GQIASPLGSYILNVKVAG++YDMEALKQLKEKGLDRSAVKELTCIGKQC+RKSFTL
Sbjct: 481 LFNCGQIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELTCIGKQCFRKSFTL 540

Query: 541 MAIVTFVGAMVSLVLVMRTREFYKGDIYKKFR-DDEVK 574
           MAIVTFVGAMVSLVLVMRTREFY+GDIYKKFR +DEVK
Sbjct: 541 MAIVTFVGAMVSLVLVMRTREFYRGDIYKKFRGEDEVK 578

BLAST of HG10018250 vs. NCBI nr
Match: XP_004147280.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN64786.1 hypothetical protein Csa_014287 [Cucumis sativus])

HSP 1 Score: 968.8 bits (2503), Expect = 2.1e-278
Identity = 504/590 (85.42%), Postives = 538/590 (91.19%), Query Frame = 0

Query: 7   ADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNL 66
           +D  H C  ++FPLQVI GRWFTVFATFLIMAGAGATYLFGVYSKQIK  LGYDQTTLNL
Sbjct: 9   SDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68

Query: 67  MGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCL 126
           MGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAV+GRIAKP+VWQMCL
Sbjct: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128

Query: 127 YICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATS 186
           YICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IY AVY DDAT+
Sbjct: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188

Query: 187 LILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKRVE 246
           LILLIGWLPAA+SV+FVFTIRRLR ERQPNEKRVFYHFLY+SIGLA+FIMIMNIVQK+V+
Sbjct: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248

Query: 247 FNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEPKII 306
           FNHTAYA SATVICVFLFLPL +VIREEL +WN KK   +P E+P    QPK IDEPKII
Sbjct: 249 FNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESP----QPKPIDEPKII 308

Query: 307 TEASKEIKEI-----ANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLG 366
           TE SK+I EI     A PPP SCFSNI  KP RGDDYTILQALLSIDM VLF+ATFCGLG
Sbjct: 309 TEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLG 368

Query: 367 TSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMM 426
           TSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGR+F+GFVSE+LLA+FKFPRPLMM
Sbjct: 369 TSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMM 428

Query: 427 TLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFG 486
           TLVLLLSC+GQLLIAFPVPGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFN G
Sbjct: 429 TLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCG 488

Query: 487 QIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVT 546
           QIASPLGSYILNVKVAG++YDMEALKQLKEKGLDRSAVKEL C+GKQCYRKSF++ AIVT
Sbjct: 489 QIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVT 548

Query: 547 FVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEE--------TVTARESK 584
           FVGAMVSLVLVMRTREFYKGDIYKKFR++E+K+E         VTARESK
Sbjct: 549 FVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 594

BLAST of HG10018250 vs. NCBI nr
Match: XP_008465591.1 (PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0051859.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK21380.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 964.1 bits (2491), Expect = 5.3e-277
Identity = 497/577 (86.14%), Postives = 531/577 (92.03%), Query Frame = 0

Query: 5   TGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTL 64
           T +D +H    ++FPLQVI GRWFTVFATFLIMAGAGATYLFGVYSKQIK  LGYDQTTL
Sbjct: 7   TSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTL 66

Query: 65  NLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQM 124
           NLMGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAVTGRIAKP+VWQM
Sbjct: 67  NLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQM 126

Query: 125 CLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDA 184
           CLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IY AVY DDA
Sbjct: 127 CLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDA 186

Query: 185 TSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKR 244
           T+LILLIGWLPAA+SV+FVFTIRRL+ ERQPNEKRVFYHFLY+SIGLA+FIMIMNIVQK+
Sbjct: 187 TALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKK 246

Query: 245 VEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEPK 304
           VEFNHTAY +SATVIC+FLFLPL IVIREEL LWNA K PP+P ENP    QPK IDEPK
Sbjct: 247 VEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENP----QPKPIDEPK 306

Query: 305 IITEASKEIKEIAN------PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 364
           IITE S +I +I N      PP  +CFSNI  KP RGDDYTILQALLSIDM VLFIATFC
Sbjct: 307 IITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIATFC 366

Query: 365 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 424
           GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGR+F+GFVSE+LLA++KFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFPRP 426

Query: 425 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 484
           LMMTLVLLLSCIGQLLIAFPVPGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 485 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 544
           N GQIASPLGSYILNVKVAG++YD+EALKQLKEKGLDRSAVKELTCIGK+CYRKSFT+  
Sbjct: 487 NCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTITT 546

Query: 545 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEE 576
           IVTF GAMVSL+LVMRTREFYKGDIYKKFR++EVK+E
Sbjct: 547 IVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKE 579

BLAST of HG10018250 vs. NCBI nr
Match: XP_023519125.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 497/578 (85.99%), Postives = 531/578 (91.87%), Query Frame = 0

Query: 3   TTTGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQT 62
           T  GAD   G   ++F L V+KGRWFTVFATFLIMAGAGATYLFGVYSKQIK TLGYDQT
Sbjct: 7   TNRGADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQT 66

Query: 63  TLNLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVW 122
           TLNLMGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAVTGRIAKP+VW
Sbjct: 67  TLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVW 126

Query: 123 QMCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGD 182
           QMCLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IYLAVYG+
Sbjct: 127 QMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGE 186

Query: 183 DATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQ 242
           DAT+LILLI WLPAALSV+F+F IR+L+ ERQPNE RVFYHFLY+SIGLA+FIM+MNI+Q
Sbjct: 187 DATALILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQ 246

Query: 243 KRVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDE 302
           K+VEFNHTAYA+SATVIC  LFLPLFIVIREELH+WN+K  PP+P ENP    +PK I E
Sbjct: 247 KKVEFNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENP---IEPK-IXE 306

Query: 303 PKIITEASKEIKEIAN----PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 362
           PKI  E SKEIK   +    PPP SCFS+I  KPARGDDYTILQALLSIDM+VLFIATFC
Sbjct: 307 PKISIEESKEIKNNPDPKPYPPPPSCFSSICSKPARGDDYTILQALLSIDMMVLFIATFC 366

Query: 363 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 422
           GLGTSLTAVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 426

Query: 423 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 482
           LMMTLVLLLSCIGQLLIAFP+PGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 483 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 542
           N GQIASPLGSYILNVKVAG+MYD EALKQLKE G+DR+AVKELTCIGK+CYRKSFTLMA
Sbjct: 487 NCGQIASPLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMA 546

Query: 543 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEET 577
           IVTFVGA+VSLVLVMRTREFY+GDIYK+FR +EVK+ET
Sbjct: 547 IVTFVGALVSLVLVMRTREFYRGDIYKRFR-EEVKQET 579

BLAST of HG10018250 vs. NCBI nr
Match: XP_023519124.1 (protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 949.1 bits (2452), Expect = 1.8e-272
Identity = 494/578 (85.47%), Postives = 527/578 (91.18%), Query Frame = 0

Query: 3   TTTGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQT 62
           T  GAD   G   ++F L V+KGRWFTVFATFLIMAGAGATYLFGVYSKQIK TLGYDQT
Sbjct: 7   TNRGADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQT 66

Query: 63  TLNLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVW 122
           TLNLMGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAVTGRIAKP+VW
Sbjct: 67  TLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVW 126

Query: 123 QMCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGD 182
           QMCLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IYLAVYG+
Sbjct: 127 QMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGE 186

Query: 183 DATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQ 242
           DAT+LILLI WLPAALSV+F+F IR+L+ ERQPNE RVFYHFLY+SIGLA+FIM+MNI+Q
Sbjct: 187 DATALILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQ 246

Query: 243 KRVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDE 302
           K+VEFNHTAYA+SATVIC  LFLPLFIVIREELH+WN+K  PP+P ENP          E
Sbjct: 247 KKVEFNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENP---------IE 306

Query: 303 PKIITEASKEIKEIAN----PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 362
           PKI  E SKEIK   +    PPP SCFS+I  KPARGDDYTILQALLSIDM+VLFIATFC
Sbjct: 307 PKISIEESKEIKNNPDPKPYPPPPSCFSSICSKPARGDDYTILQALLSIDMMVLFIATFC 366

Query: 363 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 422
           GLGTSLTAVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 426

Query: 423 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 482
           LMMTLVLLLSCIGQLLIAFP+PGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 483 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 542
           N GQIASPLGSYILNVKVAG+MYD EALKQLKE G+DR+AVKELTCIGK+CYRKSFTLMA
Sbjct: 487 NCGQIASPLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKRCYRKSFTLMA 546

Query: 543 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEET 577
           IVTFVGA+VSLVLVMRTREFY+GDIYK+FR +EVK+ET
Sbjct: 547 IVTFVGALVSLVLVMRTREFYRGDIYKRFR-EEVKQET 574

BLAST of HG10018250 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-29
Identity = 143/552 (25.91%), Postives = 243/552 (44.02%), Query Frame = 0

Query: 19  PLQVIKGRW--FTVF-ATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGA 78
           PL+ + GRW  +TV  A   I A  G  + F  YS  +K  LG  Q  LN +  + DLG 
Sbjct: 33  PLRTMFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGK 92

Query: 79  NVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCLYICVGAN-S 138
             G  SG+     P   VL   +A+ F GY + WL +T  I  P  + +    C+ A  S
Sbjct: 93  AFGWSSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP--YSLVFLCCLLAGLS 152

Query: 139 QNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATSLILLIGWL 198
             + NT   + CI +FP +R   + L   F G+S A+ +  + A+    +   +LL   +
Sbjct: 153 ICWFNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLV 212

Query: 199 PAALSVLFVF-TIRRLRVERQP--NEKRVFYHFLYVSIGLALFIMIMNIVQKRVEFNHTA 258
           P  +S   ++  + +  ++  P  + +R   H   +   LA+      ++      +   
Sbjct: 213 PLVVSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARL 272

Query: 259 YAISATVICVF-LFLPLFIVIREE-LHLWNAKKLPPMPNENPQSQSQPKSIDEPKIITEA 318
             I A V+ VF L  PL +  R+  L + NA+        +  S     +IDE K    +
Sbjct: 273 NFIGAVVLLVFPLCAPLLVYARDYFLPVINARL------NHESSGYVMLNIDELKNQKTS 332

Query: 319 SKEIKEIANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLGTSLTAVDN 378
                   +   A   + +      GD+++    +  ++  + +IA FCG    L   +N
Sbjct: 333 VSSKTGYEHMGTAKEGNTVR----LGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNN 392

Query: 379 LGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSC 438
           LGQI +SLG   +  ++ V++ S +++FGR+ S    + +  +F+  R     + LL + 
Sbjct: 393 LGQIAQSLG---QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTP 452

Query: 439 IGQLLIAF--PVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPL 498
           I   L+A       ++  A+ +IG S G       +I S+LFG        N      P+
Sbjct: 453 IAFFLLAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPI 512

Query: 499 GSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVTFVGAMV 558
           GS +L   +A  +Y+  A   +     D      + CIG+ CY K+F     ++ +G + 
Sbjct: 513 GS-LLYGYIAASIYEANASPDITPIVSD-----SIVCIGRDCYFKTFVFWGCLSILGVVS 562

Query: 559 SLVLVMRTREFY 560
           SL L +RT+  Y
Sbjct: 573 SLSLYIRTKPVY 562

BLAST of HG10018250 vs. ExPASy TrEMBL
Match: A0A0A0LXR5 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G097655 PE=4 SV=1)

HSP 1 Score: 968.8 bits (2503), Expect = 1.0e-278
Identity = 504/590 (85.42%), Postives = 538/590 (91.19%), Query Frame = 0

Query: 7   ADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNL 66
           +D  H C  ++FPLQVI GRWFTVFATFLIMAGAGATYLFGVYSKQIK  LGYDQTTLNL
Sbjct: 9   SDAGHSCRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTLNL 68

Query: 67  MGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCL 126
           MGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAV+GRIAKP+VWQMCL
Sbjct: 69  MGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVSGRIAKPKVWQMCL 128

Query: 127 YICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATS 186
           YICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IY AVY DDAT+
Sbjct: 129 YICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDATA 188

Query: 187 LILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKRVE 246
           LILLIGWLPAA+SV+FVFTIRRLR ERQPNEKRVFYHFLY+SIGLA+FIMIMNIVQK+V+
Sbjct: 189 LILLIGWLPAAISVVFVFTIRRLRSERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKKVQ 248

Query: 247 FNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEPKII 306
           FNHTAYA SATVICVFLFLPL +VIREEL +WN KK   +P E+P    QPK IDEPKII
Sbjct: 249 FNHTAYASSATVICVFLFLPLLVVIREELRIWNTKKSTSVPIESP----QPKPIDEPKII 308

Query: 307 TEASKEIKEI-----ANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLG 366
           TE SK+I EI     A PPP SCFSNI  KP RGDDYTILQALLSIDM VLF+ATFCGLG
Sbjct: 309 TEESKQITEIQKQNLATPPPESCFSNICQKPPRGDDYTILQALLSIDMFVLFVATFCGLG 368

Query: 367 TSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMM 426
           TSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGR+F+GFVSE+LLA+FKFPRPLMM
Sbjct: 369 TSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARFKFPRPLMM 428

Query: 427 TLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFG 486
           TLVLLLSC+GQLLIAFPVPGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLFN G
Sbjct: 429 TLVLLLSCVGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLFNCG 488

Query: 487 QIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVT 546
           QIASPLGSYILNVKVAG++YDMEALKQLKEKGLDRSAVKEL C+GKQCYRKSF++ AIVT
Sbjct: 489 QIASPLGSYILNVKVAGMLYDMEALKQLKEKGLDRSAVKELICMGKQCYRKSFSITAIVT 548

Query: 547 FVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEE--------TVTARESK 584
           FVGAMVSLVLVMRTREFYKGDIYKKFR++E+K+E         VTARESK
Sbjct: 549 FVGAMVSLVLVMRTREFYKGDIYKKFREEEMKKEEMVINGGGEVTARESK 594

BLAST of HG10018250 vs. ExPASy TrEMBL
Match: A0A5A7UED5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold609G00250 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.6e-277
Identity = 497/577 (86.14%), Postives = 531/577 (92.03%), Query Frame = 0

Query: 5   TGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTL 64
           T +D +H    ++FPLQVI GRWFTVFATFLIMAGAGATYLFGVYSKQIK  LGYDQTTL
Sbjct: 7   TSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTL 66

Query: 65  NLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQM 124
           NLMGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAVTGRIAKP+VWQM
Sbjct: 67  NLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQM 126

Query: 125 CLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDA 184
           CLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IY AVY DDA
Sbjct: 127 CLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDA 186

Query: 185 TSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKR 244
           T+LILLIGWLPAA+SV+FVFTIRRL+ ERQPNEKRVFYHFLY+SIGLA+FIMIMNIVQK+
Sbjct: 187 TALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKK 246

Query: 245 VEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEPK 304
           VEFNHTAY +SATVIC+FLFLPL IVIREEL LWNA K PP+P ENP    QPK IDEPK
Sbjct: 247 VEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENP----QPKPIDEPK 306

Query: 305 IITEASKEIKEIAN------PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 364
           IITE S +I +I N      PP  +CFSNI  KP RGDDYTILQALLSIDM VLFIATFC
Sbjct: 307 IITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIATFC 366

Query: 365 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 424
           GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGR+F+GFVSE+LLA++KFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFPRP 426

Query: 425 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 484
           LMMTLVLLLSCIGQLLIAFPVPGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 485 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 544
           N GQIASPLGSYILNVKVAG++YD+EALKQLKEKGLDRSAVKELTCIGK+CYRKSFT+  
Sbjct: 487 NCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTITT 546

Query: 545 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEE 576
           IVTF GAMVSL+LVMRTREFYKGDIYKKFR++EVK+E
Sbjct: 547 IVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKE 579

BLAST of HG10018250 vs. ExPASy TrEMBL
Match: A0A1S3CP80 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103503227 PE=4 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.6e-277
Identity = 497/577 (86.14%), Postives = 531/577 (92.03%), Query Frame = 0

Query: 5   TGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTL 64
           T +D +H    ++FPLQVI GRWFTVFATFLIMAGAGATYLFGVYSKQIK  LGYDQTTL
Sbjct: 7   TSSDASHPRRPLQFPLQVITGRWFTVFATFLIMAGAGATYLFGVYSKQIKSALGYDQTTL 66

Query: 65  NLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQM 124
           NLMGF KDLGANVGVLSGLVAEV PTWFVLLLGSALNFTGYFMIWLAVTGRIAKP+VWQM
Sbjct: 67  NLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPKVWQM 126

Query: 125 CLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDA 184
           CLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IY AVY DDA
Sbjct: 127 CLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYRAVYADDA 186

Query: 185 TSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKR 244
           T+LILLIGWLPAA+SV+FVFTIRRL+ ERQPNEKRVFYHFLY+SIGLA+FIMIMNIVQK+
Sbjct: 187 TALILLIGWLPAAISVVFVFTIRRLKAERQPNEKRVFYHFLYISIGLAVFIMIMNIVQKK 246

Query: 245 VEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEPK 304
           VEFNHTAY +SATVIC+FLFLPL IVIREEL LWNA K PP+P ENP    QPK IDEPK
Sbjct: 247 VEFNHTAYTLSATVICIFLFLPLLIVIREELRLWNANKSPPIPIENP----QPKPIDEPK 306

Query: 305 IITEASKEIKEIAN------PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 364
           IITE S +I +I N      PP  +CFSNI  KP RGDDYTILQALLSIDM VLFIATFC
Sbjct: 307 IITEESNQITKIQNQSPPAPPPEVACFSNICRKPPRGDDYTILQALLSIDMFVLFIATFC 366

Query: 365 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 424
           GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGR+F+GFVSE+LLA++KFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRIFAGFVSESLLARYKFPRP 426

Query: 425 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 484
           LMMTLVLLLSCIGQLLIAFPVPGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPVPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 485 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 544
           N GQIASPLGSYILNVKVAG++YD+EALKQLKEKGLDRSAVKELTCIGK+CYRKSFT+  
Sbjct: 487 NCGQIASPLGSYILNVKVAGMLYDIEALKQLKEKGLDRSAVKELTCIGKECYRKSFTITT 546

Query: 545 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEE 576
           IVTF GAMVSL+LVMRTREFYKGDIYKKFR++EVK+E
Sbjct: 547 IVTFAGAMVSLILVMRTREFYKGDIYKKFREEEVKKE 579

BLAST of HG10018250 vs. ExPASy TrEMBL
Match: A0A6J1HPV2 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464958 PE=4 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.9e-272
Identity = 493/578 (85.29%), Postives = 526/578 (91.00%), Query Frame = 0

Query: 3   TTTGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQT 62
           T  G D A G   ++F L V+KGRWFTVFATFLIMAGAGATYLFGVYSKQIK TLGYDQT
Sbjct: 7   TNRGPDAARGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQT 66

Query: 63  TLNLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVW 122
           TLNLMGF KDLGANVGVLSGLVAEV PTWFVLLLGS LNFTGYFMIWLAVTGRI KP+VW
Sbjct: 67  TLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRITKPKVW 126

Query: 123 QMCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGD 182
           QMCLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILT+IYLAVYG+
Sbjct: 127 QMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTEIYLAVYGE 186

Query: 183 DATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQ 242
           DAT+LILLI WLPAALSV+F+F IR+L+ ERQPNE RVFYHFLY+SIGLA+FIM+MNI+Q
Sbjct: 187 DATALILLIAWLPAALSVVFIFNIRKLKAERQPNETRVFYHFLYISIGLAIFIMLMNIIQ 246

Query: 243 KRVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDE 302
           K+VEFNHTAYA+SATVIC  LFLPLFIVIREELH+WN+K  PP+P ENP          E
Sbjct: 247 KKVEFNHTAYALSATVICALLFLPLFIVIREELHIWNSKINPPIPIENP---------IE 306

Query: 303 PKIITEASKEIKEIAN----PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 362
           PKI  E SKEIK   +    PPP SCFS+I +KPARGDDYTILQALLSIDM+VLFIATFC
Sbjct: 307 PKISIEESKEIKNNPDPKPYPPPPSCFSSICNKPARGDDYTILQALLSIDMMVLFIATFC 366

Query: 363 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 422
           GLGTSLTAVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 426

Query: 423 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 482
           LMMTLVLLLSCIGQLLIAFP+PGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPIPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 483 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 542
           N GQIASPLGSYILNVKVAG+MYD EALKQLKE G+DR+AVKELTCIGKQCYRKSFTLMA
Sbjct: 487 NCGQIASPLGSYILNVKVAGMMYDAEALKQLKEMGMDRAAVKELTCIGKQCYRKSFTLMA 546

Query: 543 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEET 577
           IVTFVGA+VSLVLVMRTREFY+GDIYK+FR +EVK+ET
Sbjct: 547 IVTFVGALVSLVLVMRTREFYRGDIYKRFR-EEVKQET 574

BLAST of HG10018250 vs. ExPASy TrEMBL
Match: A0A6J1I026 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469244 PE=4 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.5e-272
Identity = 493/578 (85.29%), Postives = 525/578 (90.83%), Query Frame = 0

Query: 3   TTTGADGAHGCAAIRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQT 62
           T  GAD   G   ++F L V+KGRWFTVFATFLIMAGAGATYLFGVYSKQIK TLGYDQT
Sbjct: 7   TNRGADAGRGGGLLQFALHVLKGRWFTVFATFLIMAGAGATYLFGVYSKQIKSTLGYDQT 66

Query: 63  TLNLMGFSKDLGANVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVW 122
           TLNLMGF KDLGANVGVLSGLVAEV PTWFVLLLGS LNFTGYFMIWLAVTGRIAKP+VW
Sbjct: 67  TLNLMGFFKDLGANVGVLSGLVAEVTPTWFVLLLGSVLNFTGYFMIWLAVTGRIAKPKVW 126

Query: 123 QMCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGD 182
           QMCLYICVGANSQNFANTGALVTCI NFPESRGAMMGLLKGFTGLSGAILTQIYLAVYG+
Sbjct: 127 QMCLYICVGANSQNFANTGALVTCIQNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGE 186

Query: 183 DATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQ 242
           DAT+LILLI WLPAALSV+F+F IR+L+ ER+PNE RVFYHFLYVSIGLA+FIM+MNI+Q
Sbjct: 187 DATALILLIAWLPAALSVVFIFNIRKLKAERRPNETRVFYHFLYVSIGLAIFIMLMNIIQ 246

Query: 243 KRVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDE 302
           K VEFNHTAYA+SATVIC  LFLPLFIVIREEL +WN+K  PP+P ENP          E
Sbjct: 247 KEVEFNHTAYALSATVICALLFLPLFIVIREELQIWNSKINPPIPIENP---------TE 306

Query: 303 PKIITEASKEIKEIAN----PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFC 362
           PKI T+ SKEIK   +    PPP SCFS+I  KPARGDDYTILQALLSIDM+VLFIATFC
Sbjct: 307 PKISTDESKEIKNNPDPKPYPPPPSCFSSICDKPARGDDYTILQALLSIDMMVLFIATFC 366

Query: 363 GLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 422
           GLGTSLTAVDNLGQIGESLGYPLKTV SFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP
Sbjct: 367 GLGTSLTAVDNLGQIGESLGYPLKTVKSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRP 426

Query: 423 LMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLF 482
           LMMTLVLLLSCIGQLLIAFP+PGSVYIAS+IIGFSFGAQLPLLFAIISELFGLKYFSTLF
Sbjct: 427 LMMTLVLLLSCIGQLLIAFPIPGSVYIASVIIGFSFGAQLPLLFAIISELFGLKYFSTLF 486

Query: 483 NFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMA 542
           N GQIASPLGSYILNVKVAG+MYD EALKQLKE G+DR+AVKELTCIGK+CYRKSFTLMA
Sbjct: 487 NCGQIASPLGSYILNVKVAGMMYDAEALKQLKEMGIDRAAVKELTCIGKRCYRKSFTLMA 546

Query: 543 IVTFVGAMVSLVLVMRTREFYKGDIYKKFRDDEVKEET 577
           IVTFVGA+VSLVLVMRTREFY+GDIYK+FR +EVKEET
Sbjct: 547 IVTFVGALVSLVLVMRTREFYRGDIYKRFR-EEVKEET 574

BLAST of HG10018250 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 762.7 bits (1968), Expect = 2.2e-220
Identity = 392/569 (68.89%), Postives = 456/569 (80.14%), Query Frame = 0

Query: 16  IRFPLQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGA 75
           +RF +    GRWF VFA+FLIMA AGATYLFG YSK IK TLGYDQTTLNL+GF KDLGA
Sbjct: 7   VRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGA 66

Query: 76  NVGVLSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCLYICVGANSQ 135
           NVGVLSGL+AEV PTWFVL +GSA+NF GYFMIWL VTG++AKP+VWQMCLYIC+GANSQ
Sbjct: 67  NVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQ 126

Query: 136 NFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATSLILLIGWLP 195
           NFANTGALVTC+ NFPESRG M+GLLKG+ GLSGAI TQ+Y A+YG D+ SLILLI WLP
Sbjct: 127 NFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLP 186

Query: 196 AALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKRVEFNHTAYAIS 255
           AA+S++FV+ IR  +V RQ NE  VFY FLY+SI LALF+M MNI +K+V F+  AYA S
Sbjct: 187 AAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 246

Query: 256 ATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSID-EPKIITEASKEIK 315
           AT+ C  LF+PL + +++EL +WN  KLP       + +   K +D +     + + E K
Sbjct: 247 ATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEK 306

Query: 316 EIANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLGTSLTAVDNLGQIG 375
           E       SCFS +   P RG+DYTILQALLS DM++LF+ATFCGLG+SLTAVDNLGQIG
Sbjct: 307 E-----TKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIG 366

Query: 376 ESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQLL 435
           ESLGYP  TVSSFVSLVSIWNYFGRVFSGFVSE LLAK+K PRPLMMTLVLLLSC G LL
Sbjct: 367 ESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLL 426

Query: 436 IAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYILNV 495
           IAFPVPGSVYIASI++GFSFGAQLPLLFAIISELFGLKY+STLFN GQ+ASPLGSYILNV
Sbjct: 427 IAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNV 486

Query: 496 KVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVTFVGAMVSLVLVMR 555
           +V G++YD EALKQL  +GL R  VK+LTC+G QCY+  F ++A VTF GA+VSL L +R
Sbjct: 487 RVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIR 546

Query: 556 TREFYKGDIYKKFRDDEVKEETVTARESK 584
           TREFYKGDIYKKFR+    E  +     K
Sbjct: 547 TREFYKGDIYKKFRESPESESELVPDSRK 570

BLAST of HG10018250 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 672.2 bits (1733), Expect = 3.9e-193
Identity = 343/563 (60.92%), Postives = 433/563 (76.91%), Query Frame = 0

Query: 20  LQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGANVGV 79
           +Q++ GRWF  F + LIM+ AGATY+FG+YS  IK TLGYDQTTLNL+ F KDLGANVGV
Sbjct: 15  IQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGV 74

Query: 80  LSGLVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCLYICVGANSQNFAN 139
           L+GL+ EV P WF+LL+G+ LNF GYFMIWLAVT RI+KPQVW MCLYICVGANSQ+FAN
Sbjct: 75  LAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFAN 134

Query: 140 TGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATSLILLIGWLPAALS 199
           TG+LVTC+ NFPESRG ++G+LKG+ GLSGAI+TQ+Y A YG+D   LIL+IGWLPA +S
Sbjct: 135 TGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVS 194

Query: 200 VLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQKRVEFNHTAYAISATVI 259
             F+ TIR ++V+RQ NE +VFY+FLY+S+GLA F+M++ I+ K   F  + +  SA V+
Sbjct: 195 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 254

Query: 260 CVFLFLPLFIVIREELHLWNAKKL---PPMP----NENPQSQSQPKSIDEPKIITEASKE 319
            V L LP+ +VI EE  LW  K++    P P     E P+  S     D+     E SKE
Sbjct: 255 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDD----GEESKE 314

Query: 320 IKEIANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLGTSLTAVDNLGQ 379
           + E    P  SC++ + + P RGDDYTILQAL S+DML+LF+AT CG+G +LTA+DNLGQ
Sbjct: 315 VVEKVKTP--SCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQ 374

Query: 380 IGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTLVLLLSCIGQ 439
           IG SLGYP ++VS+FVSLVSIWNY+GRV SG VSE  L K+KFPRPLM+T+VLLLSC G 
Sbjct: 375 IGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGH 434

Query: 440 LLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFGQIASPLGSYIL 499
           LLIAF VPG +Y+AS+IIGF FGAQ PLLFAIISE+FGLKY+STL+NFG +ASP+GSY+L
Sbjct: 435 LLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLL 494

Query: 500 NVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVTFVGAMVSLVLV 559
           NV+VAG +YD+EA KQ K  G  R   ++L CIG  C++ SF ++A VT  G +VS+VLV
Sbjct: 495 NVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLV 554

Query: 560 MRTREFYKGDIYKKFRDDEVKEE 576
           +RT++FYK DIYKKFR+  +  E
Sbjct: 555 IRTKKFYKSDIYKKFREKALAAE 571

BLAST of HG10018250 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 320.1 bits (819), Expect = 3.7e-87
Identity = 199/575 (34.61%), Postives = 311/575 (54.09%), Query Frame = 0

Query: 23  IKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGANVGVLSG 82
           I  RW    A   I + AG  YLFG  S  IK +L Y+Q  L+ +G +KDLG +VG ++G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 83  LVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCLYICVGANSQNFANTGA 142
            ++E++P W  LL+G+  N  GY  +WL VTGR     +W MC+ I VG N + + NTGA
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 143 LVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATSLILLIGWLPAALSVLF 202
           LV+ + NFP+SRG ++G+LKGF GL GAI++QIY  ++  +  SLIL++   PA + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVCL 192

Query: 203 VFTIRRLRVERQ--PNEKRVFYHFLYVSIGLALFIMIMNIVQKRVEFNHTAYAISATVIC 262
           +F IR +   +Q  P +   F     V + LA ++M + ++Q  V  +H    +   V+ 
Sbjct: 193 MFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVLF 252

Query: 263 VFLFLPLFIVI--------REELHLWNAKKLPPMPNENPQSQS-----------QPKSID 322
           V L +P+ + I         E         +P   ++ P  Q+           +PK +D
Sbjct: 253 VILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDVD 312

Query: 323 EPKIITEASKEIKEIAN--------PPPASCFSNIHHKPARGDDYTILQALLSIDMLVLF 382
               +  AS+  K IA+            +   N    P RG+D+T+ QAL+  D  ++F
Sbjct: 313 ----LLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIF 372

Query: 383 IATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKF 442
            +   G G+ LT +DNLGQ+ +SLGY    V   VS++SIWN+ GR+  G+ SE ++  +
Sbjct: 373 FSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRDY 432

Query: 443 KFPRPLMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKY 502
            +PRP+ M +  L+  +G +  A+  PG++YI +++IG  +GA   ++ A  SELFGLK 
Sbjct: 433 AYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKK 492

Query: 503 FSTLFNFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKS 562
           F  L+NF  +A+P GS + +  +A  +YD EA +Q      D      L C G  C+  +
Sbjct: 493 FGALYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPD--DALRCNGSICFFLT 552

Query: 563 FTLMAIVTFVGAMVSLVLVMRTREFYKGDIYKKFR 569
             +M+    +  M+S++LV RT+  Y   +Y K R
Sbjct: 553 SLIMSGFCIIACMLSMILVRRTKSVYT-HLYGKTR 578

BLAST of HG10018250 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 317.0 bits (811), Expect = 3.2e-86
Identity = 202/578 (34.95%), Postives = 318/578 (55.02%), Query Frame = 0

Query: 23  IKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGANVGVLSG 82
           I  RW    A   I + AG  YLFG  S  IK +L Y+Q  L+ +G +KDLG +VG L+G
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVGFLAG 72

Query: 83  LVAEVMPTWFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQMCLYICVGANSQNFANTGA 142
            ++E++P W  LL+GS  N  GY  +WL VTGR     +W MC+ I VG N + + NT A
Sbjct: 73  TLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYFNTAA 132

Query: 143 LVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDDATSLILLIGWLPAALSVLF 202
           LV+ + NFP+SRG ++G+LKGF GL GAIL+Q+Y  ++  D  SLI ++   P+ + V  
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHSSDRASLIFMVAVAPSVVVVPL 192

Query: 203 VFTIRRLRVERQ--PNEKRVFYHFLYVSIGLALFIMIMNIVQKRVEFNHTAYAISATVIC 262
           +F IR +   RQ   ++   F     V I LA ++M + +V+  ++ +H+       V+ 
Sbjct: 193 MFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAFTVVLF 252

Query: 263 VFLFLPLFIVIR--------------EELHLWNAKKLPP----MPNENPQ------SQSQ 322
             L +P+FI I               EE  L + +   P     P+  P+         +
Sbjct: 253 AILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSEVEDEK 312

Query: 323 PKSIDEPKIITEASKEIKEI-ANPPPASCFSNIHHK----PARGDDYTILQALLSIDMLV 382
           PK +D    + E  K I ++ A    A+    +  K    P RG+D+T+ QAL+  D  +
Sbjct: 313 PKEVDLLPAV-ERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKADFWL 372

Query: 383 LFIATFCGLGTSLTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLA 442
           +F +   G G+ LT +DNLGQ+ +SLGY    V  FVS++SIWN+ GR+  G+ SE ++ 
Sbjct: 373 IFFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--FVSMISIWNFLGRIGGGYFSELIVR 432

Query: 443 KFKFPRPLMMTLVLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGL 502
            + +PRP+ + +  L+  +G +  A+  PG+++I +++IG  +GA   ++ A  SELFGL
Sbjct: 433 DYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASELFGL 492

Query: 503 KYFSTLFNFGQIASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYR 562
           K F  L+NF  +A+P GS + +  +A  +YD EA +Q +    +   V  L C G  CY 
Sbjct: 493 KKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDV--LRCRGSICYF 552

Query: 563 KSFTLMAIVTFVGAMVSLVLVMRTREFYKGDIYKKFRD 570
            +  +M+    + A +S++LV RT+  Y  ++Y K R+
Sbjct: 553 LTSLIMSGFCLIAAALSMILVQRTKPVYT-NLYGKTRN 584

BLAST of HG10018250 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 311.2 bits (796), Expect = 1.7e-84
Identity = 193/563 (34.28%), Postives = 310/563 (55.06%), Query Frame = 0

Query: 20  LQVIKGRWFTVFATFLIMAGAGATYLFGVYSKQIKRTLGYDQTTLNLMGFSKDLGANVGV 79
           +++++ +W  + A+  I   +GA+Y FG+YS  +K T  YDQ+TL+ +   KD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 80  LSGLVAEVMPT----------------WFVLLLGSALNFTGYFMIWLAVTGRIAKPQVWQ 139
            SGL+     +                W VL +G+   F GYF+IW +VTG I KP V  
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 140 MCLYICVGANSQNFANTGALVTCILNFPESRGAMMGLLKGFTGLSGAILTQIYLAVYGDD 199
           MCL++ + A SQ F NT  +V+ + NF +  G  +G++KGF GLSGAIL Q+Y  +   D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 200 ATSLILLIGWLPAALSVLFVFTIRRLRVERQPNEKRVFYHFLYVSIGLALFIMIMNIVQK 259
             S ILL+   P  LS+L V  + R+      ++K+       VS+ +A ++MI+ I++ 
Sbjct: 181 PASFILLLAVTPTVLSLL-VMPLVRIYETSVADDKKHLNGLSAVSLIIAAYLMIIIILKN 240

Query: 260 RVEFNHTAYAISATVICVFLFLPLFIVIREELHLWNAKKLPPMPNENPQSQSQPKSIDEP 319
              F  +++A   T++C+ + L L ++I         +K   +P++     S PK+    
Sbjct: 241 --TFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEK--TVPHDYSPLISSPKATTSG 300

Query: 320 KIITEASKEIKEIANPPPASCFSNIHHKPARGDDYTILQALLSIDMLVLFIATFCGLGTS 379
              +E   ++                 +    ++  +LQA+  +   +LF+A  CG+G+ 
Sbjct: 301 NQSSEGDSKV-----------------EAGLSENLNLLQAMKKLSFWLLFLAMICGMGSG 360

Query: 380 LTAVDNLGQIGESLGYPLKTVSSFVSLVSIWNYFGRVFSGFVSETLLAKFKFPRPLMMTL 439
           L+ ++N+ QIGESL Y    ++S VSL SIWN+ GR  +G+ S+ LL K  +PRPL+M  
Sbjct: 361 LSTINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAA 420

Query: 440 VLLLSCIGQLLIAFPVPGSVYIASIIIGFSFGAQLPLLFAIISELFGLKYFSTLFNFGQI 499
            L    IG L+IA    G++Y+ S+I+G  +G+Q  L+  I SELFG+++  T+FN   +
Sbjct: 421 TLGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISV 480

Query: 500 ASPLGSYILNVKVAGIMYDMEALKQLKEKGLDRSAVKELTCIGKQCYRKSFTLMAIVTFV 559
           ASP+GSYI +V++ G +YD  A            + +  TC G  C+R SF +MA V F 
Sbjct: 481 ASPIGSYIFSVRLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFF 529

Query: 560 GAMVSLVLVMRTREFYKGDIYKK 567
           G +V++VL  RT+  Y+  + K+
Sbjct: 541 GFLVAIVLFFRTKTLYRQILVKR 529

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895725.16.8e-30193.77protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida][more]
XP_004147280.12.1e-27885.42protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis sativus] >KGN64786.1 hypothetical pr... [more]
XP_008465591.15.3e-27786.14PREDICTED: protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo] >KAA0051859.1 ... [more]
XP_023519125.11.2e-27385.99protein NUCLEAR FUSION DEFECTIVE 4-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023519124.11.8e-27285.47protein NUCLEAR FUSION DEFECTIVE 4-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
F4I9E17.7e-2925.91Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A0A0LXR51.0e-27885.42Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G09765... [more]
A0A5A7UED52.6e-27786.14Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CP802.6e-27786.14protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo OX=3656 GN=LOC103503227 ... [more]
A0A6J1HPV21.9e-27285.29protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I0265.5e-27285.29protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
Match NameE-valueIdentityDescription
AT2G28120.12.2e-22068.89Major facilitator superfamily protein [more]
AT2G39210.13.9e-19360.92Major facilitator superfamily protein [more]
AT5G14120.13.7e-8734.61Major facilitator superfamily protein [more]
AT3G01930.23.2e-8634.95Major facilitator superfamily protein [more]
AT1G74780.11.7e-8434.28Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 344..560
e-value: 1.3E-10
score: 43.0
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 26..557
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 26..271
e-value: 1.1E-90
score: 303.4
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 11..575
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 11..575
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 25..545
e-value: 1.92048E-109
score: 331.138

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10018250.1HG10018250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006817 phosphate ion transport
biological_process GO:0050896 response to stimulus
cellular_component GO:0016021 integral component of membrane