HG10017976 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10017976
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	Cystatin domain-containing protein
Location	Chr03: 28469031 .. 28469360 (-)
RNA-Seq Expression	HG10017976
Synteny	HG10017976

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCGTGGAAGAACAATTTCCCCCTTTAAAACCTATTAAGGATATCAATGATCCGCAAGTGCAAGCATTAGGAGGGTTGGCGGTGGAAGACCATAACAAGAAAACAGGGGACAATTTGAATTTCGTTCGTGTGGTCAATGGTTTGAGTGGAAAAGTTATCCTGCCTGACTTTAAGGCAGCAATATCATATGAGCTTGTGGTGGAGGCTCAAACCACTGACAAAATTAATTGGACATATGTGGCCAAGGTGTTAGAAGTTATCGGAGGTTGCCTAATAAGGCTTGACTTCATATCTTTTGAACATGTTCTCCCATACAACAAGCCCTAA

mRNA sequence

ATGAGCGTGGAAGAACAATTTCCCCCTTTAAAACCTATTAAGGATATCAATGATCCGCAAGTGCAAGCATTAGGAGGGTTGGCGGTGGAAGACCATAACAAGAAAACAGGGGACAATTTGAATTTCGTTCGTGTGGTCAATGGTTTGAGTGGAAAAGTTATCCTGCCTGACTTTAAGGCAGCAATATCATATGAGCTTGTGGTGGAGGCTCAAACCACTGACAAAATTAATTGGACATATGTGGCCAAGGTGTTAGAAGTTATCGGAGGTTGCCTAATAAGGCTTGACTTCATATCTTTTGAACATGTTCTCCCATACAACAAGCCCTAA

Coding sequence (CDS)

ATGAGCGTGGAAGAACAATTTCCCCCTTTAAAACCTATTAAGGATATCAATGATCCGCAAGTGCAAGCATTAGGAGGGTTGGCGGTGGAAGACCATAACAAGAAAACAGGGGACAATTTGAATTTCGTTCGTGTGGTCAATGGTTTGAGTGGAAAAGTTATCCTGCCTGACTTTAAGGCAGCAATATCATATGAGCTTGTGGTGGAGGCTCAAACCACTGACAAAATTAATTGGACATATGTGGCCAAGGTGTTAGAAGTTATCGGAGGTTGCCTAATAAGGCTTGACTTCATATCTTTTGAACATGTTCTCCCATACAACAAGCCCTAA

Protein sequence

MSVEEQFPPLKPIKDINDPQVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPDFKAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLIRLDFISFEHVLPYNKP

Homology

[Close]

BLAST of HG10017976 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 119.4 bits (298), Expect = 1.9e-23
Identity = 68/111 (61.26%), Postives = 80/111 (72.07%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDPQVQALGGLAVED-HNKKTGDNLNFVRVVNGL-SGKVILPDF 60
           MS+EE    LKPI DINDP VQALG LAVE+ ++K +G+ L FVRVVNGL S K I P F
Sbjct: 1   MSIEESRHRLKPIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGF 60

Query: 61  KAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLIRLDFISFEHVLPYNKP 110
              I Y LV+EA+T ++INWTY  K+ EV GG LIR  F+SFE VLPY KP
Sbjct: 61  TEVILYHLVLEAKTNEEINWTYATKLEEVSGG-LIRHVFLSFEPVLPYYKP 110

[Close]

BLAST of HG10017976 vs. NCBI nr
Match: XP_031739698.1 (uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetical protein Csa_012480 [Cucumis sativus])

HSP 1 Score: 99.0 bits (245), Expect = 2.7e-17
Identity = 59/97 (60.82%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDP-QVQA-LGGLAVEDHNKKTGDNLNFVRVVNGL-SGKVILP- 60
           MSVEE +   KPI+DIND  QVQ+ +G LAVEDHNKKTGDNL  V VVNGL SG  + P 
Sbjct: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69

Query: 61  DFKAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLI 94
                I Y L+VEA+T + INWTYVAK+LE+  GC I
Sbjct: 70  STHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 106

[Close]

BLAST of HG10017976 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 97.8 bits (242), Expect = 6.0e-17
Identity = 55/103 (53.40%), Postives = 64/103 (62.14%), Query Frame = 0


Query: 9   PLKPIKDINDPQVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILP-DFKAAISYELV 68
           P +PIKDINDPQVQ +G L V+DHNKKTG+NL FVRVVNGL    + P        YELV
Sbjct: 6   PFEPIKDINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNGLFAGFVDPFSPLGGTVYELV 65

Query: 69  VEAQTTDKINWTYVAKV--LEVIGGCLIRLDFISFEHVLPYNK 109
           +EA    KINWTY  KV     I    +  +F SFE VL Y +
Sbjct: 66  LEAINAGKINWTYKVKVSASTTIRPLGVVYNFQSFEPVLQYEE 108

[Close]

BLAST of HG10017976 vs. NCBI nr
Match: TYK05428.1 (Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 97.8 bits (242), Expect = 6.0e-17
Identity = 56/97 (57.73%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 1  MSVEEQFPPLKPIKDINDP-QVQA-LGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPD- 60
          MSVEE +   KPI++IND  QVQ+ +G LAVEDHNKKTG+NL FV VVNGL  +V +   
Sbjct: 1  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 61 -FKAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLI 94
               I Y L+VEA+T + INWTYVAK+LE+  GC I
Sbjct: 61 CTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 97

[Close]

BLAST of HG10017976 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 89.7 bits (221), Expect = 1.6e-14
Identity = 56/111 (50.45%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDP-QVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILP-DF 60
           MS+   F   KPI+DIND  +VQ LG L V+DHNKKTG+NL FVRVVNGL+     P   
Sbjct: 1   MSIMSAF---KPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISP 60

Query: 61  KAAISYELVVEAQTTDKINWTYVAKVLE---VIGGCLIRLDFISFEHVLPY 107
              + YELV+EA TT KINWTY A+V +   +     +   F SF+ VL Y
Sbjct: 61  LGGVLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

[Close]

BLAST of HG10017976 vs. ExPASy Swiss-Prot
Match: Q10J94 (Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0429000 PE=2 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 1.9e-05
Identity = 30/75 (40.00%), Postives = 38/75 (50.67%), Query Frame = 0


Query: 12  PIKDINDPQVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPDFKAAISYELVVEAQ 71
           PI D+ DP +Q LGG AVE H   + D L F RV +G    V      + ++Y LVV A 
Sbjct: 37  PITDVGDPHIQELGGWAVERHASLSSDGLRFRRVTSGEQQVV------SGMNYRLVVSAS 96

Query: 72  TTDKINWTYVAKVLE 87
                  +YVA V E
Sbjct: 97  DPAGATASYVAVVYE 105

[Close]

BLAST of HG10017976 vs. ExPASy Swiss-Prot
Match: Q84WT8 (Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 1.6e-04
Identity = 27/76 (35.53%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 11  KPIKDINDPQVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPDFKAAISYELVVEA 70
           KPIK+++DP V A+   A+E+HNK++ + L FV+VV G +  V      +   Y+L + A
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV------SGTKYDLKIAA 92

Query: 71  QTTDKINWTYVAKVLE 87
           +        Y A V+E
Sbjct: 93  KDGGGKIKNYEAVVVE 102

[Close]

BLAST of HG10017976 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.3e-25
Identity = 67/111 (60.36%), Postives = 78/111 (70.27%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDPQVQALGGLAVEDHNK-KTGDNLNFVRVVNGL-SGKVILPDF 60
           MS+EE    LKPI DINDP VQ +G LAVEDH+    G+ L FVRVVNGL S K I P F
Sbjct: 1   MSIEEIHHRLKPIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGF 60

Query: 61  KAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLIRLDFISFEHVLPYNKP 110
              I Y LV+EA+T ++INWTY  K+ +V  GCLIR  F+SFE VLPYNKP
Sbjct: 61  TEVILYHLVLEAKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPYNKP 111

[Close]

BLAST of HG10017976 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 9.4e-24
Identity = 68/111 (61.26%), Postives = 80/111 (72.07%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDPQVQALGGLAVED-HNKKTGDNLNFVRVVNGL-SGKVILPDF 60
           MS+EE    LKPI DINDP VQALG LAVE+ ++K +G+ L FVRVVNGL S K I P F
Sbjct: 1   MSIEESRHRLKPIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGF 60

Query: 61  KAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLIRLDFISFEHVLPYNKP 110
              I Y LV+EA+T ++INWTY  K+ EV GG LIR  F+SFE VLPY KP
Sbjct: 61  TEVILYHLVLEAKTNEEINWTYATKLEEVSGG-LIRHVFLSFEPVLPYYKP 110

[Close]

BLAST of HG10017976 vs. ExPASy TrEMBL
Match: A0A0A0L3W1 (Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE=4 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 2.1e-23
Identity = 68/113 (60.18%), Postives = 80/113 (70.80%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDP-QVQA-LGGLAVEDHNKKTGDNLNFVRVVNGL-SGKVILP- 60
           MSVEE +   KPI+DIND  QVQ+ +G LAVEDHNKKTGDNL  V VVNGL SG  + P 
Sbjct: 10  MSVEEAYHTSKPIEDINDDLQVQSIIGQLAVEDHNKKTGDNLELVDVVNGLRSGIFVQPG 69

Query: 61  DFKAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLIRLDFISFEHVLPYNKP 110
                I Y L+VEA+T + INWTYVAK+LE+  GC IR +F SFE +LPY  P
Sbjct: 70  STHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRIRYEFKSFEPLLPYYNP 122

[Close]

BLAST of HG10017976 vs. ExPASy TrEMBL
Match: A0A5D3C2L1 (Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001230 PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.9e-17
Identity = 56/97 (57.73%), Postives = 69/97 (71.13%), Query Frame = 0


Query: 1  MSVEEQFPPLKPIKDINDP-QVQA-LGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPD- 60
          MSVEE +   KPI++IND  QVQ+ +G LAVEDHNKKTG+NL FV VVNGL  +V +   
Sbjct: 1  MSVEEAYHTSKPIENINDDLQVQSIIGKLAVEDHNKKTGENLEFVDVVNGLRSEVFVQPG 60

Query: 61 -FKAAISYELVVEAQTTDKINWTYVAKVLEVIGGCLI 94
               I Y L+VEA+T + INWTYVAK+LE+  GC I
Sbjct: 61 CTHEGILYHLLVEAKTIEGINWTYVAKLLELYVGCRI 97

[Close]

BLAST of HG10017976 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 8.0e-15
Identity = 56/111 (50.45%), Postives = 69/111 (62.16%), Query Frame = 0


Query: 1   MSVEEQFPPLKPIKDINDP-QVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILP-DF 60
           MS+   F   KPI+DIND  +VQ LG L V+DHNKKTG+NL FVRVVNGL+     P   
Sbjct: 1   MSIMSAF---KPIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISP 60

Query: 61  KAAISYELVVEAQTTDKINWTYVAKVLE---VIGGCLIRLDFISFEHVLPY 107
              + YELV+EA TT KINWTY A+V +   +     +   F SF+ VL Y
Sbjct: 61  LGGVLYELVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

[Close]

BLAST of HG10017976 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.1e-05
Identity = 27/76 (35.53%), Postives = 43/76 (56.58%), Query Frame = 0


Query: 11  KPIKDINDPQVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPDFKAAISYELVVEA 70
           KPIK+++DP V A+   A+E+HNK++ + L FV+VV G +  V      +   Y+L + A
Sbjct: 33  KPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEGTTQVV------SGTKYDLKIAA 92

Query: 71  QTTDKINWTYVAKVLE 87
           +        Y A V+E
Sbjct: 93  KDGGGKIKNYEAVVVE 102

[Close]

BLAST of HG10017976 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.3e-04
Identity = 25/73 (34.25%), Postives = 39/73 (53.42%), Query Frame = 0


Query: 12 PIKDINDPQVQALGGLAVEDHNKKTGDNLNFVRVVNGLSGKVILPDFKAAISYELVVEAQ 71
          PI ++ DPQV  +G  AV ++NK++   L F  VV+G +  V      +  +Y L V A 
Sbjct: 33 PISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSGETQVV------SGTNYRLKVAAN 92

Query: 72 TTDKINWTYVAKV 85
            D ++  Y+A V
Sbjct: 93 DGDGVSKNYLAIV 99

The following BLAST results are available for this feature:

BLAST of HG10017976 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN54823.1	1.9e-23	61.26	hypothetical protein Csa_012607 [Cucumis sativus]	[more]
XP_031739698.1	2.7e-17	60.82	uncharacterized protein LOC116403239 [Cucumis sativus] >KAE8649718.1 hypothetica...	[more]
KAE8649716.1	6.0e-17	53.40	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
TYK05428.1	6.0e-17	57.73	Proteinase inhibitor I25, cystatin [Cucumis melo var. makuwa]	[more]
XP_008442277.1	1.6e-14	50.45	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]

BLAST of HG10017976 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10J94	1.9e-05	40.00	Cysteine proteinase inhibitor 8 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03...	[more]
Q84WT8	1.6e-04	35.53	Cysteine proteinase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=CYS4 PE=3 SV=...	[more]

BLAST of HG10017976 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L191	1.3e-25	60.36	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]
A0A0A0KZ98	9.4e-24	61.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]
A0A0A0L3W1	2.1e-23	60.18	Proteinase inhibitor I25, cystatin OS=Cucumis sativus OX=3659 GN=Csa_4G508490 PE...	[more]
A0A5D3C2L1	2.9e-17	57.73	Proteinase inhibitor I25, cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A5A7TJK0	8.0e-15	50.45	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]

BLAST of HG10017976 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16500.1	1.1e-05	35.53	Cystatin/monellin superfamily protein	[more]
AT5G47550.1	1.3e-04	34.25	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 13..87 e-value: 2.8E-12 score: 46.8
None	No IPR available	GENE3D	3.10.450.10		coord: 9..104 e-value: 6.3E-14 score: 53.8
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 10..104
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 12..87
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 10..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10017976.1	HG10017976.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity