HG10017387 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10017387
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionDNA-directed RNA polymerase subunit alpha
LocationChr03: 13933029 .. 13934507 (-)
RNA-Seq ExpressionHG10017387
SyntenyHG10017387
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAACCCATACCAAGAATCGGTTCACGTAGAAATGGACGTATTAGCTCACGTAAAAGTACGCGTAGAATACCAAAGGGCGTTATTCATGTTCAAGCAAGTTTCAACAATACAATTGTGACTGTTACAGATGTACGGGGTCGGGTAATTTCTTGGTCCTCCGCCGGTACGTGTGGATTCAAGGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCAGGAAATGCTATTCGGGCAGTAGTGGATCAAGGTATGCAACGAGCCGAAGTTATGATAAAGGGCCCTGGTCTCGGAAGAGATGCAGCATTAAGAGCTATTCGTAGAAGCGGTATACTATTAAGTTTCATACGGGATGTAACCCCTATGCCACATAATGGCTGTAGGCCCCCTAAAAAACGACGTGTGTAAAAATAAACATTGAAGAGAAATTTCAAGAGAAATAAATAATTCAATGATTTGATCAAAAAATATTACTATGGTTCGAGAGAAAATAAGAGTATCGACTCGGACACTAAAGTGGAAGTGTGTTGAATCAAGAGCAGACAGTAAGCGTCTTTATTATGGACGCTTTATTCTGTCTCCACTTATGAAGGGGCTACTATAGGCATTGCGATGCGAAAAGCTTTACTTGGAGAAATAGAGGGAACATGTATCACACGTGCAAAATCTGAAAAAATCCCACATGAATACTCTACCATAGTCGGTATTCAAGAATCAGTACATGAAATTTTAATGAATTTGAAAGAAATTGTATTGAGAAGTAATCTGTATGGAACTCGTGATGCGTCTATTTGTGTCAAGGGTCCTGGATGCGTAACTGCTCAAGACATCATCTTACCACCTTCGGTAGAAATCGTTGATAATACACAGCATATAGCTAACCTAACGGAGCCAATTAATTTGTGTATTGAATTAAAGATCGAGAGGAATCGCGGATATCGTATACAAACGCCAAATAATTTTCAAGACGCAAGTTATCCTATAGATGCTATATTCATGCCTGTTCGAAATGCGAATCATAGTATTCATTCTTATGTAAATGGGAATGAAAAACAAGAGATACTTTTTCTCGAAATATGGACCAATGGAAGTTTAACTCCTAAAGAAGCACTTCATGAAGCCTCTCGGAATTTGATTGATTTATTTATTCCTTTTTTACATGCGGAAGAAGAAAAAGAAAACTTCCATTTAAAAAACAATAAGCAGAAAGTTACTTTACCCCTTTTTACTTTTCATGAGAAATTGGCTAAACTAAGGAAAAAGAAAAAAGAAATAGCATTAAAATATATTTTTATTGACCAATCAGAATTGCCTCCTAGGATCTATAATTGTCTCAAAAGGTCCAATATACATACATTATTTGACCTTTTGAATAATAGTCCAGACGAACTTATGAAAATAAAACATTTTCGCATAGAAGACGTAAAACATATATTAGACATTCTCGAAATGGAAAAGAATTTTCCATAA

mRNA sequence

ATGGCAAAACCCATACCAAGAATCGGTTCACGTAGAAATGGACGTATTAGCTCACGTAAAAGTACGCGTAGAATACCAAAGGGCGTTATTCATGTTCAAGCAAGTTTCAACAATACAATTGTGACTGTTACAGATGTACGGGGTCGGGTAATTTCTTGGTCCTCCGCCGGTACGTGTGGATTCAAGGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCAGGAAATGCTATTCGGGCAGTAGTGGATCAAGGTATGCAACGAGCCGAAGTTATGATAAAGGGCCCTGGTCTCGGAAGAGATGCAGCATTAAGAGCTATTCGTAGAAGCGGGGCTACTATAGGCATTGCGATGCGAAAAGCTTTACTTGGAGAAATAGAGGGAACATGTATCACACGTGCAAAATCTGAAAAAATCCCACATGAATACTCTACCATAGTCGGTATTCAAGAATCAGTACATGAAATTTTAATGAATTTGAAAGAAATTGTATTGAGAAGTAATCTGTATGGAACTCGTGATGCGTCTATTTGTGTCAAGGGTCCTGGATGCGTAACTGCTCAAGACATCATCTTACCACCTTCGGTAGAAATCGTTGATAATACACAGCATATAGCTAACCTAACGGAGCCAATTAATTTGTGTATTGAATTAAAGATCGAGAGGAATCGCGGATATCGTATACAAACGCCAAATAATTTTCAAGACGCAAGTTATCCTATAGATGCTATATTCATGCCTGTTCGAAATGCGAATCATAGTATTCATTCTTATGTAAATGGGAATGAAAAACAAGAGATACTTTTTCTCGAAATATGGACCAATGGAAGTTTAACTCCTAAAGAAGCACTTCATGAAGCCTCTCGGAATTTGATTGATTTATTTATTCCTTTTTTACATGCGGAAGAAGAAAAAGAAAACTTCCATTTAAAAAACAATAAGCAGAAAGTTACTTTACCCCTTTTTACTTTTCATGAGAAATTGGCTAAACTAAGGAAAAAGAAAAAAGAAATAGCATTAAAATATATTTTTATTGACCAATCAGAATTGCCTCCTAGGATCTATAATTGTCTCAAAAGGTCCAATATACATACATTATTTGACCTTTTGAATAATAGTCCAGACGAACTTATGAAAATAAAACATTTTCGCATAGAAGACGTAAAACATATATTAGACATTCTCGAAATGGAAAAGAATTTTCCATAA

Coding sequence (CDS)

ATGGCAAAACCCATACCAAGAATCGGTTCACGTAGAAATGGACGTATTAGCTCACGTAAAAGTACGCGTAGAATACCAAAGGGCGTTATTCATGTTCAAGCAAGTTTCAACAATACAATTGTGACTGTTACAGATGTACGGGGTCGGGTAATTTCTTGGTCCTCCGCCGGTACGTGTGGATTCAAGGGTACAAGAAGAGGGACACCATTTGCCGCTCAAACCGCAGCAGGAAATGCTATTCGGGCAGTAGTGGATCAAGGTATGCAACGAGCCGAAGTTATGATAAAGGGCCCTGGTCTCGGAAGAGATGCAGCATTAAGAGCTATTCGTAGAAGCGGGGCTACTATAGGCATTGCGATGCGAAAAGCTTTACTTGGAGAAATAGAGGGAACATGTATCACACGTGCAAAATCTGAAAAAATCCCACATGAATACTCTACCATAGTCGGTATTCAAGAATCAGTACATGAAATTTTAATGAATTTGAAAGAAATTGTATTGAGAAGTAATCTGTATGGAACTCGTGATGCGTCTATTTGTGTCAAGGGTCCTGGATGCGTAACTGCTCAAGACATCATCTTACCACCTTCGGTAGAAATCGTTGATAATACACAGCATATAGCTAACCTAACGGAGCCAATTAATTTGTGTATTGAATTAAAGATCGAGAGGAATCGCGGATATCGTATACAAACGCCAAATAATTTTCAAGACGCAAGTTATCCTATAGATGCTATATTCATGCCTGTTCGAAATGCGAATCATAGTATTCATTCTTATGTAAATGGGAATGAAAAACAAGAGATACTTTTTCTCGAAATATGGACCAATGGAAGTTTAACTCCTAAAGAAGCACTTCATGAAGCCTCTCGGAATTTGATTGATTTATTTATTCCTTTTTTACATGCGGAAGAAGAAAAAGAAAACTTCCATTTAAAAAACAATAAGCAGAAAGTTACTTTACCCCTTTTTACTTTTCATGAGAAATTGGCTAAACTAAGGAAAAAGAAAAAAGAAATAGCATTAAAATATATTTTTATTGACCAATCAGAATTGCCTCCTAGGATCTATAATTGTCTCAAAAGGTCCAATATACATACATTATTTGACCTTTTGAATAATAGTCCAGACGAACTTATGAAAATAAAACATTTTCGCATAGAAGACGTAAAACATATATTAGACATTCTCGAAATGGAAAAGAATTTTCCATAA

Protein sequence

MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCGFKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGATIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEMEKNFP
Homology
BLAST of HG10017387 vs. NCBI nr
Match: PRQ15682.1 (putative DNA-directed RNA polymerase [Rosa chinensis])

HSP 1 Score: 691.0 bits (1782), Expect = 6.0e-195
Identity = 361/465 (77.63%), Postives = 382/465 (82.15%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAKPIP+IGSRRN RI SRK+ RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKPIPKIGSRRNRRIGSRKNARRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA------ 120
           FKGTRRGTPFAAQTAAGNAIR VVDQGMQRAEVMIKGPGLGRDAALRAIRRSG       
Sbjct: 61  FKGTRRGTPFAAQTAAGNAIRTVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFIR 120

Query: 121 --------------------------------------------------------TIGI 180
                                                                   TIGI
Sbjct: 121 DVTPMPHNGCRPPKKRRKVTVSTRTLQWKCVESRADSKRLYYGRFILAPLMKGQADTIGI 180

Query: 181 AMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDAS 240
           AMR+ALLGEIEGTCITRA+SEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTR+AS
Sbjct: 181 AMRRALLGEIEGTCITRARSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRNAS 240

Query: 241 ICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQD 300
           ICVKGPG VTAQDIILPPSVEIVDNTQHIANLTEPINLCI+L+IERNRGYRI+TPNNFQD
Sbjct: 241 ICVKGPGYVTAQDIILPPSVEIVDNTQHIANLTEPINLCIQLQIERNRGYRIKTPNNFQD 300

Query: 301 ASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFI 360
            SYPIDA+FMPVRNANHSIHSYVNGNEKQEILF+EIWTNGSLTPKEALHEASRNLIDLFI
Sbjct: 301 GSYPIDAVFMPVRNANHSIHSYVNGNEKQEILFIEIWTNGSLTPKEALHEASRNLIDLFI 360

Query: 361 PFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYN 404
           PFLHAEE  ENFH +NN+ KVTLPLFTFH+K A  RK K EI LKYIFIDQSELPPR+YN
Sbjct: 361 PFLHAEE--ENFHFENNQHKVTLPLFTFHDKFANPRKSKTEITLKYIFIDQSELPPRVYN 420

BLAST of HG10017387 vs. NCBI nr
Match: KAF4397623.1 (hypothetical protein G4B88_027363 [Cannabis sativa])

HSP 1 Score: 673.7 bits (1737), Expect = 9.9e-190
Identity = 355/465 (76.34%), Postives = 377/465 (81.08%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAK IPR GSR+N    SRK+ RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKAIPRSGSRKNRHTGSRKNARRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA------ 120
           FKGTRRGTPFAAQTAAGNAIR VVDQGMQ+AEVMIKGPGLGRDAALRAIRRSG       
Sbjct: 61  FKGTRRGTPFAAQTAAGNAIRTVVDQGMQQAEVMIKGPGLGRDAALRAIRRSGILLSFIR 120

Query: 121 --------------------------------------------------------TIGI 180
                                                                   TIGI
Sbjct: 121 DVTPMPHNGCRPPKKRQKVIVSTRPLQWKCVESRADSKRLYYGRFILAPLMRGQADTIGI 180

Query: 181 AMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDAS 240
            MR+ALLGEIEGTCITRAKS+KIPHEYS +VGIQESVHEILMNLKEIVLRSNLYGTR+AS
Sbjct: 181 VMRRALLGEIEGTCITRAKSDKIPHEYSNLVGIQESVHEILMNLKEIVLRSNLYGTREAS 240

Query: 241 ICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQD 300
           ICVKGPG VTAQDIILP SVEIVDNTQHIANLTEPINLCIEL+IERNRGYRI+TPNNFQ+
Sbjct: 241 ICVKGPGYVTAQDIILPSSVEIVDNTQHIANLTEPINLCIELQIERNRGYRIKTPNNFQN 300

Query: 301 ASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFI 360
            SYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDL I
Sbjct: 301 GSYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLLI 360

Query: 361 PFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYN 404
           PFLHAEE  ENFHL+NN+ K TLPLF FH ++AK RK KKEIALKYIFIDQSELPPR+YN
Sbjct: 361 PFLHAEE--ENFHLENNQHKGTLPLFDFHGRVAKPRKTKKEIALKYIFIDQSELPPRVYN 420

BLAST of HG10017387 vs. NCBI nr
Match: TXG68058.1 (hypothetical protein EZV62_009333 [Acer yangbiense])

HSP 1 Score: 667.9 bits (1722), Expect = 5.4e-188
Identity = 348/431 (80.74%), Postives = 367/431 (85.15%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAK IPR GSRR GR+ SRKS RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKSIPRSGSRRTGRVGSRKSARRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA------ 120
           F+GTRRGTPFAAQTAAGNAIRAV DQGMQRAEVMIKGPGLGRDAALRAIRRSG       
Sbjct: 61  FRGTRRGTPFAAQTAAGNAIRAVTDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFWK 120

Query: 121 ---------------------------TIGIAMRKALLGEIEGTCITRAKSEKIPHEYST 180
                                      TIGIAMR+ALLGEIEGTCITRAKSEKIPHEYST
Sbjct: 121 CVESRADSKRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKSEKIPHEYST 180

Query: 181 IVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHI 240
           IVGIQESVHEILMNLKEIVLRSNLYGTRDA ICVKGPG VTAQDI+LPPSVEIVDNTQHI
Sbjct: 181 IVGIQESVHEILMNLKEIVLRSNLYGTRDALICVKGPGYVTAQDILLPPSVEIVDNTQHI 240

Query: 241 ANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQ 300
           ANLTEP + CI L+IERNRGY I+TPNNFQD SYPIDAIFMPVRNANHSIHSY NGNEKQ
Sbjct: 241 ANLTEPTDFCIGLQIERNRGYNIKTPNNFQDGSYPIDAIFMPVRNANHSIHSYGNGNEKQ 300

Query: 301 EILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFH 360
           EILFLEIWTNG LTPKEALHEASRNLIDLFIPFL + E  EN H +NN+ KV+LP  TFH
Sbjct: 301 EILFLEIWTNGGLTPKEALHEASRNLIDLFIPFLQSAE--ENLHFENNQYKVSLPYLTFH 360

Query: 361 EKLAKLRKKKKEIALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRI 399
           +++AKL+KKKKEIA K IFIDQSEL PRIYNCLK+SNIHTLFDLLNNS ++LMKI+HFRI
Sbjct: 361 DRVAKLKKKKKEIAWKSIFIDQSELSPRIYNCLKKSNIHTLFDLLNNSQEDLMKIEHFRI 420

BLAST of HG10017387 vs. NCBI nr
Match: KAF1876904.1 (hypothetical protein Lal_00033201 [Lupinus albus])

HSP 1 Score: 657.9 bits (1696), Expect = 5.6e-185
Identity = 341/405 (84.20%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAKP P++GSR+NGRI SRK  R+IPKGVIHVQASFNNTIVTVTDV GRVISWSSAGTCG
Sbjct: 1   MAKPTPKMGSRKNGRIGSRKHARKIPKGVIHVQASFNNTIVTVTDVGGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA--TIGI 120
           FKGTRRGTPFAAQTAA NAI  V DQGMQRAEVMIKGPGLGRDAALRAIRRSG   TIGI
Sbjct: 61  FKGTRRGTPFAAQTAAANAILTVADQGMQRAEVMIKGPGLGRDAALRAIRRSGQADTIGI 120

Query: 121 AMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDAS 180
           AMR+ LLGEIEGTCITRAKSEKIPHEYST+VGIQESVHEILMNLKEIVLRSNLYGTRDAS
Sbjct: 121 AMRRVLLGEIEGTCITRAKSEKIPHEYSTLVGIQESVHEILMNLKEIVLRSNLYGTRDAS 180

Query: 181 ICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQD 240
           I VKGPG VTAQDI+LPPSVEIVDNTQHIANLTEPIN  I L+I RNRGYRI+T   FQD
Sbjct: 181 IFVKGPGYVTAQDILLPPSVEIVDNTQHIANLTEPINFYIGLQIARNRGYRIKTLTKFQD 240

Query: 241 ASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFI 300
            SYPIDA+FM VRN NHSIHSYVNGNEKQEILFLEIWTNGSLTPKEAL EASRNLIDLFI
Sbjct: 241 RSYPIDAVFMSVRNVNHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALFEASRNLIDLFI 300

Query: 301 PFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYN 360
           PFLHAEEE  NF  +NN+ KV LP FTF +   K RK KKEIALK IFIDQ EL PRIYN
Sbjct: 301 PFLHAEEENLNF--ENNQHKVNLPPFTFDDLFEKRRKNKKEIALKSIFIDQFELSPRIYN 360

Query: 361 CLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEMEKNF 404
           CLKRSNIHTLFDLLNNS ++LMK++HFR+EDVK ILDILE+EK+F
Sbjct: 361 CLKRSNIHTLFDLLNNSQEDLMKMEHFRVEDVKDILDILEIEKHF 403

BLAST of HG10017387 vs. NCBI nr
Match: AES88253.2 (DNA-directed RNA polymerase subunit alpha [Medicago truncatula])

HSP 1 Score: 653.3 bits (1684), Expect = 1.4e-183
Identity = 344/443 (77.65%), Postives = 372/443 (83.97%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAK IP+IGSR+NGRI SRK  R+IPKGVI+VQASFNNTIVTVTDVRGRVISWSSAG+CG
Sbjct: 1   MAKSIPKIGSRKNGRIGSRKHPRKIPKGVIYVQASFNNTIVTVTDVRGRVISWSSAGSCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRS-------- 120
           FKGTRRGTPFAAQTAA NAIR VVDQGMQRA V+IKGPGLGRDAALRAI RS        
Sbjct: 61  FKGTRRGTPFAAQTAAANAIRTVVDQGMQRAVVIIKGPGLGRDAALRAIARSEKVKVSAR 120

Query: 121 ------------------------------GATIGIAMRKALLGEIEGTCITRAKSEKIP 180
                                           TIGIAMR+ LLGEIEGTCITRAKSEKIP
Sbjct: 121 TLQWKCVESRVDSKRLYYGRFILSPLRKGQADTIGIAMRRILLGEIEGTCITRAKSEKIP 180

Query: 181 HEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVD 240
           HEYSTIVGIQES+HEILMNLKEIVL+SNLYGTRDASIC KGPG VTAQDIILPPSVEIVD
Sbjct: 181 HEYSTIVGIQESIHEILMNLKEIVLKSNLYGTRDASICFKGPGYVTAQDIILPPSVEIVD 240

Query: 241 NTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVN 300
           NTQHIANLTEPINLCIEL+IER RGYRI+T NN QD SY IDA+FMPVRNANHSIHSYVN
Sbjct: 241 NTQHIANLTEPINLCIELQIERKRGYRIKTLNNIQDGSYTIDAVFMPVRNANHSIHSYVN 300

Query: 301 GNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLP 360
           GNEKQEILFLEIWTNGSLTPKEAL+EASRNLIDLFIPFLHAEEE  NF  +NN+ KVTLP
Sbjct: 301 GNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEENLNF--ENNQHKVTLP 360

Query: 361 LFTFHEKLA--KLRKKKKEIALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELM 404
           LFTFH++L   KLRK KKEIALK IFIDQ EL PRIYNCLK+SNIHTL +LLN S ++LM
Sbjct: 361 LFTFHDRLLKDKLRKNKKEIALKSIFIDQLELSPRIYNCLKKSNIHTLLELLNKSQEDLM 420

BLAST of HG10017387 vs. ExPASy Swiss-Prot
Match: Q4VZK3 (DNA-directed RNA polymerase subunit alpha OS=Cucumis sativus OX=3659 GN=rpoA PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 2.4e-162
Identity = 286/303 (94.39%), Postives = 290/303 (95.71%), Query Frame = 0

Query: 101 GRDAALRAIRRSGATIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILM 160
           GR      ++  G TIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILM
Sbjct: 30  GRFILSPLMKGQGDTIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILM 89

Query: 161 NLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIEL 220
           NLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANL EPINLCIEL
Sbjct: 90  NLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLMEPINLCIEL 149

Query: 221 KIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSL 280
           KIERNRGY IQTPNNFQDASYP+DAIFMPVRN NHSIHSYVNGNEKQEILFLEIWTNGSL
Sbjct: 150 KIERNRGYHIQTPNNFQDASYPMDAIFMPVRNVNHSIHSYVNGNEKQEILFLEIWTNGSL 209

Query: 281 TPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEI 340
           TPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNK+KVTLPLFTFHEKLAKLRKKKKEI
Sbjct: 210 TPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKKKVTLPLFTFHEKLAKLRKKKKEI 269

Query: 341 ALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEME 400
           ALKYIFIDQSELPPRIYNCLKR NIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEME
Sbjct: 270 ALKYIFIDQSELPPRIYNCLKRCNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEME 329

Query: 401 KNF 404
           KNF
Sbjct: 330 KNF 332

BLAST of HG10017387 vs. ExPASy Swiss-Prot
Match: Q49KW6 (DNA-directed RNA polymerase subunit alpha OS=Eucalyptus globulus subsp. globulus OX=71271 GN=rpoA PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-140
Identity = 251/289 (86.85%), Postives = 267/289 (92.39%), Query Frame = 0

Query: 115 TIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT 174
           TIGIAMR+ALLGEIEGTCITRAK EKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT
Sbjct: 44  TIGIAMRRALLGEIEGTCITRAKFEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT 103

Query: 175 RDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPN 234
           R+ASICVKGPGCVTAQDIILP SVEIVDNTQ+IANLTEPINLCI L+IERNRGY I+TP 
Sbjct: 104 RNASICVKGPGCVTAQDIILPTSVEIVDNTQYIANLTEPINLCIGLQIERNRGYHIKTPK 163

Query: 235 NFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLI 294
           NFQD SYPIDA+FMPVRNANHSIHSY NGNEKQEILFLEIWTNGSLTPKEALHEASRNLI
Sbjct: 164 NFQDGSYPIDAVFMPVRNANHSIHSYGNGNEKQEILFLEIWTNGSLTPKEALHEASRNLI 223

Query: 295 DLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 354
           DLFIPFLHAEE  EN HL+NN+ KVTLP FTFH++LAKLRK KKEIALK IFIDQ ELPP
Sbjct: 224 DLFIPFLHAEE--ENLHLENNQYKVTLPFFTFHDRLAKLRKTKKEIALKSIFIDQFELPP 283

Query: 355 RIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEMEKNF 404
           R YNCLKR NIHTLFDLLN S ++LMK+++FRIEDVK ILD LE+EK F
Sbjct: 284 RTYNCLKRYNIHTLFDLLNKSQEDLMKMEYFRIEDVKQILDSLEIEKAF 330

BLAST of HG10017387 vs. ExPASy Swiss-Prot
Match: Q2PMQ3 (DNA-directed RNA polymerase subunit alpha OS=Glycine max OX=3847 GN=rpoA PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-140
Identity = 250/291 (85.91%), Postives = 271/291 (93.13%), Query Frame = 0

Query: 115 TIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT 174
           TIGIAMR+ LLGEIEGTCITR KSEKIPHEYSTI+GI+ESVHEILMNLKEIVLRSNLYGT
Sbjct: 44  TIGIAMRRVLLGEIEGTCITRVKSEKIPHEYSTIIGIEESVHEILMNLKEIVLRSNLYGT 103

Query: 175 RDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPN 234
           RDASIC KGPG VTAQDIILPPSVEIVDNTQHIAN+TEP+NLCIELKIERNRGYRI+T  
Sbjct: 104 RDASICFKGPGYVTAQDIILPPSVEIVDNTQHIANVTEPVNLCIELKIERNRGYRIKTLK 163

Query: 235 NFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLI 294
           NFQD SYPIDA FMPVRN NHSIHSYVNGNEKQEILFLEIWTNGSLTPKEAL+EASRNLI
Sbjct: 164 NFQDGSYPIDATFMPVRNVNHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALYEASRNLI 223

Query: 295 DLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLA--KLRKKKKEIALKYIFIDQSEL 354
           DLFIPFLHAEE  +NF+L+NN+ KVTLPLFTFH+ LA  KLRKKKKEIALK IFIDQ EL
Sbjct: 224 DLFIPFLHAEE--DNFNLENNQHKVTLPLFTFHDILAKEKLRKKKKEIALKSIFIDQLEL 283

Query: 355 PPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEMEKNF 404
           PPRIYNCLKRSNIHTL +LLNNS ++L+KI+HFR+EDVK+ILD LE+EK+F
Sbjct: 284 PPRIYNCLKRSNIHTLLELLNNSQEDLLKIEHFRVEDVKYILDFLEIEKHF 332

BLAST of HG10017387 vs. ExPASy Swiss-Prot
Match: Q09WY5 (DNA-directed RNA polymerase subunit alpha OS=Morus indica OX=248361 GN=rpoA PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 2.0e-140
Identity = 252/299 (84.28%), Postives = 269/299 (89.97%), Query Frame = 0

Query: 101 GRDAALRAIRRSGATIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILM 160
           GR      +R    TIGIAMR+ALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILM
Sbjct: 30  GRFLLAPLMRGQADTIGIAMRRALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILM 89

Query: 161 NLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIEL 220
           NLKEIVLRSNLYGTRDASICVKGPG VTAQDIILPPSVEIVDNTQHIANLTEPI LCIEL
Sbjct: 90  NLKEIVLRSNLYGTRDASICVKGPGYVTAQDIILPPSVEIVDNTQHIANLTEPITLCIEL 149

Query: 221 KIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSL 280
           +IERNRGYRI+TPNNFQ+ SYPIDA+FMPVRNANHSIHSYVNGNE QEILFLEIWTNGSL
Sbjct: 150 QIERNRGYRIKTPNNFQNGSYPIDAVFMPVRNANHSIHSYVNGNENQEILFLEIWTNGSL 209

Query: 281 TPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEI 340
           TPKEALHEASRNLIDLFIPF HAEE     HL+NN+ K TLPLF FH +LAK RK KKEI
Sbjct: 210 TPKEALHEASRNLIDLFIPFFHAEE-----HLENNQHKGTLPLFNFHGRLAKPRKTKKEI 269

Query: 341 ALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEM 400
           ALKYI+IDQSELPPR+YNCLKRSNI+T  +LLNNS +ELMKI+ FRIEDVKHILD+LE+
Sbjct: 270 ALKYIYIDQSELPPRVYNCLKRSNINTFLELLNNSQEELMKIQDFRIEDVKHILDVLEI 323

BLAST of HG10017387 vs. ExPASy Swiss-Prot
Match: Q9MTJ3 (DNA-directed RNA polymerase subunit alpha OS=Oenothera elata subsp. hookeri OX=85636 GN=rpoA PE=3 SV=2)

HSP 1 Score: 486.5 bits (1251), Expect = 2.9e-136
Identity = 245/289 (84.78%), Postives = 261/289 (90.31%), Query Frame = 0

Query: 115 TIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT 174
           TIGIAMR+ALLGEIEGTCITRAK EKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT
Sbjct: 44  TIGIAMRRALLGEIEGTCITRAKFEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT 103

Query: 175 RDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPN 234
            +ASICVKGPGCVTAQDI+LPPSVEI+DNTQHIA+L EPI+LCI L+IERNRGY I++PN
Sbjct: 104 CNASICVKGPGCVTAQDILLPPSVEIIDNTQHIASLMEPIHLCIGLQIERNRGYHIKSPN 163

Query: 235 NFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLI 294
           NFQD SYPIDA+FMPVRNANHSIHSY N NEKQEILFLEIWTNGSLTPKEALHEASRNLI
Sbjct: 164 NFQDGSYPIDAVFMPVRNANHSIHSYGNQNEKQEILFLEIWTNGSLTPKEALHEASRNLI 223

Query: 295 DLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPP 354
           DLF+PFLHAEE  EN HL  NK  VT PLFTFH+KLAKLRK K EIALK IFIDQ ELPP
Sbjct: 224 DLFLPFLHAEE--ENLHLGKNKYNVTFPLFTFHKKLAKLRKPKNEIALKSIFIDQLELPP 283

Query: 355 RIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEMEKNF 404
           RIYN LK S IHTL DLLN S ++LMK+KHFRIEDVK ILDILE+EK F
Sbjct: 284 RIYNSLKGSKIHTLSDLLNKSQEDLMKMKHFRIEDVKQILDILEIEKAF 330

BLAST of HG10017387 vs. ExPASy TrEMBL
Match: A0A2P6P177 (Multifunctional fusion protein OS=Rosa chinensis OX=74649 GN=rps11 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 2.9e-195
Identity = 361/465 (77.63%), Postives = 382/465 (82.15%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAKPIP+IGSRRN RI SRK+ RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKPIPKIGSRRNRRIGSRKNARRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA------ 120
           FKGTRRGTPFAAQTAAGNAIR VVDQGMQRAEVMIKGPGLGRDAALRAIRRSG       
Sbjct: 61  FKGTRRGTPFAAQTAAGNAIRTVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFIR 120

Query: 121 --------------------------------------------------------TIGI 180
                                                                   TIGI
Sbjct: 121 DVTPMPHNGCRPPKKRRKVTVSTRTLQWKCVESRADSKRLYYGRFILAPLMKGQADTIGI 180

Query: 181 AMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDAS 240
           AMR+ALLGEIEGTCITRA+SEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTR+AS
Sbjct: 181 AMRRALLGEIEGTCITRARSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRNAS 240

Query: 241 ICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQD 300
           ICVKGPG VTAQDIILPPSVEIVDNTQHIANLTEPINLCI+L+IERNRGYRI+TPNNFQD
Sbjct: 241 ICVKGPGYVTAQDIILPPSVEIVDNTQHIANLTEPINLCIQLQIERNRGYRIKTPNNFQD 300

Query: 301 ASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFI 360
            SYPIDA+FMPVRNANHSIHSYVNGNEKQEILF+EIWTNGSLTPKEALHEASRNLIDLFI
Sbjct: 301 GSYPIDAVFMPVRNANHSIHSYVNGNEKQEILFIEIWTNGSLTPKEALHEASRNLIDLFI 360

Query: 361 PFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYN 404
           PFLHAEE  ENFH +NN+ KVTLPLFTFH+K A  RK K EI LKYIFIDQSELPPR+YN
Sbjct: 361 PFLHAEE--ENFHFENNQHKVTLPLFTFHDKFANPRKSKTEITLKYIFIDQSELPPRVYN 420

BLAST of HG10017387 vs. ExPASy TrEMBL
Match: A0A7J6HQR3 (Plastid-encoded RNA polymerase subunit alpha OS=Cannabis sativa OX=3483 GN=G4B88_027363 PE=3 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.8e-190
Identity = 355/465 (76.34%), Postives = 377/465 (81.08%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAK IPR GSR+N    SRK+ RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKAIPRSGSRKNRHTGSRKNARRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA------ 120
           FKGTRRGTPFAAQTAAGNAIR VVDQGMQ+AEVMIKGPGLGRDAALRAIRRSG       
Sbjct: 61  FKGTRRGTPFAAQTAAGNAIRTVVDQGMQQAEVMIKGPGLGRDAALRAIRRSGILLSFIR 120

Query: 121 --------------------------------------------------------TIGI 180
                                                                   TIGI
Sbjct: 121 DVTPMPHNGCRPPKKRQKVIVSTRPLQWKCVESRADSKRLYYGRFILAPLMRGQADTIGI 180

Query: 181 AMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDAS 240
            MR+ALLGEIEGTCITRAKS+KIPHEYS +VGIQESVHEILMNLKEIVLRSNLYGTR+AS
Sbjct: 181 VMRRALLGEIEGTCITRAKSDKIPHEYSNLVGIQESVHEILMNLKEIVLRSNLYGTREAS 240

Query: 241 ICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQD 300
           ICVKGPG VTAQDIILP SVEIVDNTQHIANLTEPINLCIEL+IERNRGYRI+TPNNFQ+
Sbjct: 241 ICVKGPGYVTAQDIILPSSVEIVDNTQHIANLTEPINLCIELQIERNRGYRIKTPNNFQN 300

Query: 301 ASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFI 360
            SYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDL I
Sbjct: 301 GSYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLLI 360

Query: 361 PFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYN 404
           PFLHAEE  ENFHL+NN+ K TLPLF FH ++AK RK KKEIALKYIFIDQSELPPR+YN
Sbjct: 361 PFLHAEE--ENFHLENNQHKGTLPLFDFHGRVAKPRKTKKEIALKYIFIDQSELPPRVYN 420

BLAST of HG10017387 vs. ExPASy TrEMBL
Match: A0A5C7IG80 (Plastid-encoded RNA polymerase subunit alpha OS=Acer yangbiense OX=1000413 GN=EZV62_009333 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.6e-188
Identity = 348/431 (80.74%), Postives = 367/431 (85.15%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAK IPR GSRR GR+ SRKS RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKSIPRSGSRRTGRVGSRKSARRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA------ 120
           F+GTRRGTPFAAQTAAGNAIRAV DQGMQRAEVMIKGPGLGRDAALRAIRRSG       
Sbjct: 61  FRGTRRGTPFAAQTAAGNAIRAVTDQGMQRAEVMIKGPGLGRDAALRAIRRSGILLSFWK 120

Query: 121 ---------------------------TIGIAMRKALLGEIEGTCITRAKSEKIPHEYST 180
                                      TIGIAMR+ALLGEIEGTCITRAKSEKIPHEYST
Sbjct: 121 CVESRADSKRLYYGRFILSPLMKGQADTIGIAMRRALLGEIEGTCITRAKSEKIPHEYST 180

Query: 181 IVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVDNTQHI 240
           IVGIQESVHEILMNLKEIVLRSNLYGTRDA ICVKGPG VTAQDI+LPPSVEIVDNTQHI
Sbjct: 181 IVGIQESVHEILMNLKEIVLRSNLYGTRDALICVKGPGYVTAQDILLPPSVEIVDNTQHI 240

Query: 241 ANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQ 300
           ANLTEP + CI L+IERNRGY I+TPNNFQD SYPIDAIFMPVRNANHSIHSY NGNEKQ
Sbjct: 241 ANLTEPTDFCIGLQIERNRGYNIKTPNNFQDGSYPIDAIFMPVRNANHSIHSYGNGNEKQ 300

Query: 301 EILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFH 360
           EILFLEIWTNG LTPKEALHEASRNLIDLFIPFL + E  EN H +NN+ KV+LP  TFH
Sbjct: 301 EILFLEIWTNGGLTPKEALHEASRNLIDLFIPFLQSAE--ENLHFENNQYKVSLPYLTFH 360

Query: 361 EKLAKLRKKKKEIALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRI 399
           +++AKL+KKKKEIA K IFIDQSEL PRIYNCLK+SNIHTLFDLLNNS ++LMKI+HFRI
Sbjct: 361 DRVAKLKKKKKEIAWKSIFIDQSELSPRIYNCLKKSNIHTLFDLLNNSQEDLMKIEHFRI 420

BLAST of HG10017387 vs. ExPASy TrEMBL
Match: A0A6A5MQ34 (Plastid-encoded RNA polymerase subunit alpha OS=Lupinus albus OX=3870 GN=Lal_00033201 PE=3 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.7e-185
Identity = 341/405 (84.20%), Postives = 361/405 (89.14%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAKP P++GSR+NGRI SRK  R+IPKGVIHVQASFNNTIVTVTDV GRVISWSSAGTCG
Sbjct: 1   MAKPTPKMGSRKNGRIGSRKHARKIPKGVIHVQASFNNTIVTVTDVGGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSGA--TIGI 120
           FKGTRRGTPFAAQTAA NAI  V DQGMQRAEVMIKGPGLGRDAALRAIRRSG   TIGI
Sbjct: 61  FKGTRRGTPFAAQTAAANAILTVADQGMQRAEVMIKGPGLGRDAALRAIRRSGQADTIGI 120

Query: 121 AMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDAS 180
           AMR+ LLGEIEGTCITRAKSEKIPHEYST+VGIQESVHEILMNLKEIVLRSNLYGTRDAS
Sbjct: 121 AMRRVLLGEIEGTCITRAKSEKIPHEYSTLVGIQESVHEILMNLKEIVLRSNLYGTRDAS 180

Query: 181 ICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQD 240
           I VKGPG VTAQDI+LPPSVEIVDNTQHIANLTEPIN  I L+I RNRGYRI+T   FQD
Sbjct: 181 IFVKGPGYVTAQDILLPPSVEIVDNTQHIANLTEPINFYIGLQIARNRGYRIKTLTKFQD 240

Query: 241 ASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFI 300
            SYPIDA+FM VRN NHSIHSYVNGNEKQEILFLEIWTNGSLTPKEAL EASRNLIDLFI
Sbjct: 241 RSYPIDAVFMSVRNVNHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALFEASRNLIDLFI 300

Query: 301 PFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKKKEIALKYIFIDQSELPPRIYN 360
           PFLHAEEE  NF  +NN+ KV LP FTF +   K RK KKEIALK IFIDQ EL PRIYN
Sbjct: 301 PFLHAEEENLNF--ENNQHKVNLPPFTFDDLFEKRRKNKKEIALKSIFIDQFELSPRIYN 360

Query: 361 CLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILEMEKNF 404
           CLKRSNIHTLFDLLNNS ++LMK++HFR+EDVK ILDILE+EK+F
Sbjct: 361 CLKRSNIHTLFDLLNNSQEDLMKMEHFRVEDVKDILDILEIEKHF 403

BLAST of HG10017387 vs. ExPASy TrEMBL
Match: G7JH57 (Plastid-encoded RNA polymerase subunit alpha OS=Medicago truncatula OX=3880 GN=MTR_4g051380 PE=3 SV=2)

HSP 1 Score: 653.3 bits (1684), Expect = 6.7e-184
Identity = 344/443 (77.65%), Postives = 372/443 (83.97%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAK IP+IGSR+NGRI SRK  R+IPKGVI+VQASFNNTIVTVTDVRGRVISWSSAG+CG
Sbjct: 1   MAKSIPKIGSRKNGRIGSRKHPRKIPKGVIYVQASFNNTIVTVTDVRGRVISWSSAGSCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRS-------- 120
           FKGTRRGTPFAAQTAA NAIR VVDQGMQRA V+IKGPGLGRDAALRAI RS        
Sbjct: 61  FKGTRRGTPFAAQTAAANAIRTVVDQGMQRAVVIIKGPGLGRDAALRAIARSEKVKVSAR 120

Query: 121 ------------------------------GATIGIAMRKALLGEIEGTCITRAKSEKIP 180
                                           TIGIAMR+ LLGEIEGTCITRAKSEKIP
Sbjct: 121 TLQWKCVESRVDSKRLYYGRFILSPLRKGQADTIGIAMRRILLGEIEGTCITRAKSEKIP 180

Query: 181 HEYSTIVGIQESVHEILMNLKEIVLRSNLYGTRDASICVKGPGCVTAQDIILPPSVEIVD 240
           HEYSTIVGIQES+HEILMNLKEIVL+SNLYGTRDASIC KGPG VTAQDIILPPSVEIVD
Sbjct: 181 HEYSTIVGIQESIHEILMNLKEIVLKSNLYGTRDASICFKGPGYVTAQDIILPPSVEIVD 240

Query: 241 NTQHIANLTEPINLCIELKIERNRGYRIQTPNNFQDASYPIDAIFMPVRNANHSIHSYVN 300
           NTQHIANLTEPINLCIEL+IER RGYRI+T NN QD SY IDA+FMPVRNANHSIHSYVN
Sbjct: 241 NTQHIANLTEPINLCIELQIERKRGYRIKTLNNIQDGSYTIDAVFMPVRNANHSIHSYVN 300

Query: 301 GNEKQEILFLEIWTNGSLTPKEALHEASRNLIDLFIPFLHAEEEKENFHLKNNKQKVTLP 360
           GNEKQEILFLEIWTNGSLTPKEAL+EASRNLIDLFIPFLHAEEE  NF  +NN+ KVTLP
Sbjct: 301 GNEKQEILFLEIWTNGSLTPKEALYEASRNLIDLFIPFLHAEEENLNF--ENNQHKVTLP 360

Query: 361 LFTFHEKLA--KLRKKKKEIALKYIFIDQSELPPRIYNCLKRSNIHTLFDLLNNSPDELM 404
           LFTFH++L   KLRK KKEIALK IFIDQ EL PRIYNCLK+SNIHTL +LLN S ++LM
Sbjct: 361 LFTFHDRLLKDKLRKNKKEIALKSIFIDQLELSPRIYNCLKKSNIHTLLELLNKSQEDLM 420

BLAST of HG10017387 vs. TAIR 10
Match: ATCG00740.1 (RNA polymerase subunit alpha )

HSP 1 Score: 462.2 bits (1188), Expect = 4.2e-130
Identity = 227/286 (79.37%), Postives = 255/286 (89.16%), Query Frame = 0

Query: 115 TIGIAMRKALLGEIEGTCITRAKSEKIPHEYSTIVGIQESVHEILMNLKEIVLRSNLYGT 174
           TIGIAMR+ALLGEIEGTCITRAKSE IPH+YS I GIQESVHEILMNL EIVLRSNLYGT
Sbjct: 44  TIGIAMRRALLGEIEGTCITRAKSENIPHDYSNIAGIQESVHEILMNLNEIVLRSNLYGT 103

Query: 175 RDASICVKGPGCVTAQDIILPPSVEIVDNTQHIANLTEPINLCIELKIERNRGYRIQTPN 234
           R+A ICV+GPG +TA+DIILPP+VEI+DNTQHIA LTEPI+LCIELKIERNRGY ++  N
Sbjct: 104 RNALICVQGPGYITARDIILPPAVEIIDNTQHIATLTEPIDLCIELKIERNRGYSLKMSN 163

Query: 235 NFQDASYPIDAIFMPVRNANHSIHSYVNGNEKQEILFLEIWTNGSLTPKEALHEASRNLI 294
           NF+D SYPIDA+FMPV NANHSIHSY NGNEKQEILFLEIWTNGSLTPKEALH+ASRNLI
Sbjct: 164 NFEDRSYPIDAVFMPVENANHSIHSYGNGNEKQEILFLEIWTNGSLTPKEALHQASRNLI 223

Query: 295 DLFIPFLHAEEEKENFHLKNNKQKVTLPLFTFHEKLAKLRKKK--KEIALKYIFIDQSEL 354
           +LFIPFLH EE  E F+L+NN+ +VTLP F FH +L  LRKKK  KE+A +YIFIDQ EL
Sbjct: 224 NLFIPFLHVEE--ETFYLENNQHQVTLPFFPFHNRLVNLRKKKKTKELAFQYIFIDQLEL 283

Query: 355 PPRIYNCLKRSNIHTLFDLLNNSPDELMKIKHFRIEDVKHILDILE 399
           PPRIYNCLK+SNIHTL DLLNNS ++L+KI+HF +EDVK ILDILE
Sbjct: 284 PPRIYNCLKKSNIHTLLDLLNNSQEDLIKIEHFHVEDVKKILDILE 327

BLAST of HG10017387 vs. TAIR 10
Match: ATCG00750.1 (ribosomal protein S11 )

HSP 1 Score: 199.1 bits (505), Expect = 6.6e-51
Identity = 104/113 (92.04%), Postives = 107/113 (94.69%), Query Frame = 0

Query: 1   MAKPIPRIGSRRNGRISSRKSTRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60
           MAKPI RIGSR+N R  SRK+ RRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG
Sbjct: 1   MAKPILRIGSRKNTRSGSRKNVRRIPKGVIHVQASFNNTIVTVTDVRGRVISWSSAGTCG 60

Query: 61  FKGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVMIKGPGLGRDAALRAIRRSG 114
           F+GTRRGTPFAAQTAAGNAIRAVVDQGMQRAEV IKGPGLGRDAALRAIRRSG
Sbjct: 61  FRGTRRGTPFAAQTAAGNAIRAVVDQGMQRAEVRIKGPGLGRDAALRAIRRSG 113

BLAST of HG10017387 vs. TAIR 10
Match: AT3G52580.1 (Ribosomal protein S11 family protein )

HSP 1 Score: 51.2 bits (121), Expect = 2.2e-06
Identity = 37/99 (37.37%), Postives = 50/99 (50.51%), Query Frame = 0

Query: 28  GVIHVQASFNNTIVTVTDVRGRVISWSSAGTCGFKGTR-RGTPFAAQTAAGNAIRAVVDQ 87
           GV+HV ASFN+T + VTD+ GR       G    K  R   +P+AA  AA +  +   + 
Sbjct: 28  GVVHVFASFNDTFIHVTDLSGRETLVRITGGMKVKADRDESSPYAAMLAAQDVAQRCKEL 87

Query: 88  GMQRAEVMI--------KGPGLGRDAALRAIRRSGATIG 118
           G+    V +        K PG G  +ALRA+ RSG  IG
Sbjct: 88  GITAIHVKLRATGGNKTKTPGPGAQSALRALARSGMKIG 126

BLAST of HG10017387 vs. TAIR 10
Match: AT2G36160.1 (Ribosomal protein S11 family protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.9e-06
Identity = 36/99 (36.36%), Postives = 50/99 (50.51%), Query Frame = 0

Query: 28  GVIHVQASFNNTIVTVTDVRGRVISWSSAGTCGFKGTR-RGTPFAAQTAAGNAIRAVVDQ 87
           GV+H+ ASFN+T + VTD+ GR       G    K  R   +P+AA  AA +  +   + 
Sbjct: 28  GVVHIFASFNDTFIHVTDLSGRETLVRITGGMKVKADRDESSPYAAMLAAQDVAQRCKEL 87

Query: 88  GMQRAEVMI--------KGPGLGRDAALRAIRRSGATIG 118
           G+    V +        K PG G  +ALRA+ RSG  IG
Sbjct: 88  GITAMHVKLRATGGNKTKTPGPGAQSALRALARSGMKIG 126

BLAST of HG10017387 vs. TAIR 10
Match: AT3G11510.1 (Ribosomal protein S11 family protein )

HSP 1 Score: 50.8 bits (120), Expect = 2.9e-06
Identity = 36/99 (36.36%), Postives = 50/99 (50.51%), Query Frame = 0

Query: 28  GVIHVQASFNNTIVTVTDVRGRVISWSSAGTCGFKGTR-RGTPFAAQTAAGNAIRAVVDQ 87
           GV+H+ ASFN+T + VTD+ GR       G    K  R   +P+AA  AA +  +   + 
Sbjct: 28  GVVHIFASFNDTFIHVTDLSGRETLVRITGGMKVKADRDESSPYAAMLAAQDVAQRCKEL 87

Query: 88  GMQRAEVMI--------KGPGLGRDAALRAIRRSGATIG 118
           G+    V +        K PG G  +ALRA+ RSG  IG
Sbjct: 88  GITAMHVKLRATGGNKTKTPGPGAQSALRALARSGMKIG 126

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
PRQ15682.16.0e-19577.63putative DNA-directed RNA polymerase [Rosa chinensis][more]
KAF4397623.19.9e-19076.34hypothetical protein G4B88_027363 [Cannabis sativa][more]
TXG68058.15.4e-18880.74hypothetical protein EZV62_009333 [Acer yangbiense][more]
KAF1876904.15.6e-18584.20hypothetical protein Lal_00033201 [Lupinus albus][more]
AES88253.21.4e-18377.65DNA-directed RNA polymerase subunit alpha [Medicago truncatula][more]
Match NameE-valueIdentityDescription
Q4VZK32.4e-16294.39DNA-directed RNA polymerase subunit alpha OS=Cucumis sativus OX=3659 GN=rpoA PE=... [more]
Q49KW61.1e-14086.85DNA-directed RNA polymerase subunit alpha OS=Eucalyptus globulus subsp. globulus... [more]
Q2PMQ31.1e-14085.91DNA-directed RNA polymerase subunit alpha OS=Glycine max OX=3847 GN=rpoA PE=3 SV... [more]
Q09WY52.0e-14084.28DNA-directed RNA polymerase subunit alpha OS=Morus indica OX=248361 GN=rpoA PE=3... [more]
Q9MTJ32.9e-13684.78DNA-directed RNA polymerase subunit alpha OS=Oenothera elata subsp. hookeri OX=8... [more]
Match NameE-valueIdentityDescription
A0A2P6P1772.9e-19577.63Multifunctional fusion protein OS=Rosa chinensis OX=74649 GN=rps11 PE=3 SV=1[more]
A0A7J6HQR34.8e-19076.34Plastid-encoded RNA polymerase subunit alpha OS=Cannabis sativa OX=3483 GN=G4B88... [more]
A0A5C7IG802.6e-18880.74Plastid-encoded RNA polymerase subunit alpha OS=Acer yangbiense OX=1000413 GN=EZ... [more]
A0A6A5MQ342.7e-18584.20Plastid-encoded RNA polymerase subunit alpha OS=Lupinus albus OX=3870 GN=Lal_000... [more]
G7JH576.7e-18477.65Plastid-encoded RNA polymerase subunit alpha OS=Medicago truncatula OX=3880 GN=M... [more]
Match NameE-valueIdentityDescription
ATCG00740.14.2e-13079.37RNA polymerase subunit alpha [more]
ATCG00750.16.6e-5192.04ribosomal protein S11 [more]
AT3G52580.12.2e-0637.37Ribosomal protein S11 family protein [more]
AT2G36160.12.9e-0636.36Ribosomal protein S11 family protein [more]
AT3G11510.12.9e-0636.36Ribosomal protein S11 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011263DNA-directed RNA polymerase, RpoA/D/Rpb3-typeSMARTSM00662rpoldneu2coord: 107..303
e-value: 5.4E-14
score: 62.5
IPR011263DNA-directed RNA polymerase, RpoA/D/Rpb3-typePFAMPF01193RNA_pol_Lcoord: 109..296
e-value: 3.3E-10
score: 40.7
IPR036643DNA-directed RNA polymerase, insert domain superfamilyGENE3D2.170.120.12coord: 130..247
e-value: 2.7E-35
score: 122.9
IPR036643DNA-directed RNA polymerase, insert domain superfamilySUPERFAMILY56553Insert subdomain of RNA polymerase alpha subunitcoord: 130..248
IPR011773DNA-directed RNA polymerase, alpha subunitTIGRFAMTIGR02027TIGR02027coord: 113..398
e-value: 1.2E-76
score: 255.7
IPR011773DNA-directed RNA polymerase, alpha subunitPANTHERPTHR32108DNA-DIRECTED RNA POLYMERASE SUBUNIT ALPHAcoord: 89..402
IPR001971Ribosomal protein S11PFAMPF00411Ribosomal_S11coord: 28..118
e-value: 1.1E-35
score: 122.4
IPR001971Ribosomal protein S11HAMAPMF_01310Ribosomal_S11coord: 11..140
score: 23.232132
IPR036967Ribosomal protein S11 superfamilyGENE3D3.30.420.80Ribosomal protein S11coord: 10..122
e-value: 9.4E-33
score: 115.2
NoneNo IPR availableGENE3D1.10.150.20coord: 339..402
e-value: 1.3E-12
score: 49.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableCDDcd06928RNAP_alpha_NTDcoord: 113..300
e-value: 8.39267E-88
score: 263.561
NoneNo IPR availableSUPERFAMILY53137Translational machinery componentscoord: 24..117
NoneNo IPR availableSUPERFAMILY47789C-terminal domain of RNA polymerase alpha subunitcoord: 341..398
IPR011262DNA-directed RNA polymerase, insert domainPFAMPF01000RNA_pol_A_baccoord: 137..229
e-value: 7.8E-16
score: 58.4
IPR011260RNA polymerase, alpha subunit, C-terminalPFAMPF03118RNA_pol_A_CTDcoord: 336..398
e-value: 2.6E-13
score: 49.5
IPR036603RNA polymerase, RBP11-like subunitSUPERFAMILY55257RBP11-like subunits of RNA polymerasecoord: 111..302

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10017387.1HG10017387.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
biological_process GO:0006412 translation
cellular_component GO:0009507 chloroplast
cellular_component GO:0015934 large ribosomal subunit
cellular_component GO:0005840 ribosome
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0019843 rRNA binding
molecular_function GO:0003735 structural constituent of ribosome