HG10017336 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10017336
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	RNase H domain-containing protein
Location	Chr03: 13230375 .. 13230776 (+)
RNA-Seq Expression	HG10017336
Synteny	HG10017336

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGGTTATGGTGGGGTTATTAGTACTCCAACTGGGTGCTTGGTGGGTGCTATGCATGGTTTCAAGAATATATCTCTGAGTCCGTTAGGAGCTAAAGCTTTGACTATTTACGAGGGCCTTCGTTTGGCCATTAGAATGGAGTTACCGCATATTTTGGTATTATCTGATTCTTTAATTGTTATTCAGATTCTTAGAGGAGAGGTAAAGAACCGAGTGGAAGTGGCAACTTATGTGGCTGACATCCAGGATATGACGGTTCACTGCAGATCTATCACATTTCAGCATGTGCCTCGTTCCTTTAATATTGTGGCGCATGATTTGGCTAGAACGGAAAGCTTGGAGAATTGTGCTTTGTGGTGTGATGATTTCTCTTGGGAAATTGTCAAAAAAACACAGTGA

mRNA sequence

ATGGTGGGTTATGGTGGGGTTATTAGTACTCCAACTGGGTGCTTGGTGGGTGCTATGCATGGTTTCAAGAATATATCTCTGAGTCCGTTAGGAGCTAAAGCTTTGACTATTTACGAGGGCCTTCGTTTGGCCATTAGAATGGAGTTACCGCATATTTTGGTATTATCTGATTCTTTAATTGTTATTCAGATTCTTAGAGGAGAGGTAAAGAACCGAGTGGAAGTGGCAACTTATGTGGCTGACATCCAGGATATGACGGTTCACTGCAGATCTATCACATTTCAGCATGTGCCTCGTTCCTTTAATATTGTGGCGCATGATTTGGCTAGAACGGAAAGCTTGGAGAATTGTGCTTTGTGGTGTGATGATTTCTCTTGGGAAATTGTCAAAAAAACACAGTGA

Coding sequence (CDS)

ATGGTGGGTTATGGTGGGGTTATTAGTACTCCAACTGGGTGCTTGGTGGGTGCTATGCATGGTTTCAAGAATATATCTCTGAGTCCGTTAGGAGCTAAAGCTTTGACTATTTACGAGGGCCTTCGTTTGGCCATTAGAATGGAGTTACCGCATATTTTGGTATTATCTGATTCTTTAATTGTTATTCAGATTCTTAGAGGAGAGGTAAAGAACCGAGTGGAAGTGGCAACTTATGTGGCTGACATCCAGGATATGACGGTTCACTGCAGATCTATCACATTTCAGCATGTGCCTCGTTCCTTTAATATTGTGGCGCATGATTTGGCTAGAACGGAAAGCTTGGAGAATTGTGCTTTGTGGTGTGATGATTTCTCTTGGGAAATTGTCAAAAAAACACAGTGA

Protein sequence

MVGYGGVISTPTGCLVGAMHGFKNISLSPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTVHCRSITFQHVPRSFNIVAHDLARTESLENCALWCDDFSWEIVKKTQ

Homology

[Close]

BLAST of HG10017336 vs. NCBI nr
Match: TXG67779.1 (hypothetical protein EZV62_009054 [Acer yangbiense])

HSP 1 Score: 63.9 bits (154), Expect = 1.2e-06
Identity = 36/94 (38.30%), Postives = 55/94 (58.51%), Query Frame = 0


Query: 32  AKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTVHCRS 91
           A+A  IYEG+ LA    L  + + SDSL V+++  GE+  R +V   + DIQ +    R 
Sbjct: 187 AEAKAIYEGILLATTSGLQSLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 246

Query: 92  ITFQHVPRSFNIVAHDLARTE-SLENCALWCDDF 125
           I+  ++PRS N+VAH++AR    LEN  +  D +
Sbjct: 247 ISISYIPRSCNMVAHEVARRAIGLENSVIMYDPY 280

[Close]

BLAST of HG10017336 vs. NCBI nr
Match: TXG71894.1 (hypothetical protein EZV62_000473 [Acer yangbiense])

HSP 1 Score: 60.8 bits (146), Expect = 1.0e-05
Identity = 36/94 (38.30%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 32  AKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTVHCRS 91
           A+A  IYEGL LA    L  + + SDSL V+++  GE+  R +V   + DIQ +    R 
Sbjct: 290 AEAKVIYEGLLLATSSGLQPLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 349

Query: 92  ITFQHVPRSFNIVAHDLARTE-SLENCALWCDDF 125
            +  ++PRS N VAH++AR    LEN  +  D +
Sbjct: 350 TSISYIPRSCNRVAHEVARRAIGLENSVIMYDPY 383

[Close]

BLAST of HG10017336 vs. NCBI nr
Match: MBA0801636.1 (hypothetical protein [Gossypium harknessii])

HSP 1 Score: 59.7 bits (143), Expect = 2.2e-05
Identity = 32/88 (36.36%), Postives = 53/88 (60.23%), Query Frame = 0


Query: 28  SPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTV 87
           S   A+ +   + +++ I  + P I++  DSL +I+  + + +NR +V  Y+ DIQ  T 
Sbjct: 22  SAFAAEVIACRKAVQIGIEKQWPTIIIEVDSLAIIKKCKNKSQNRSQVGVYIHDIQQRTG 81

Query: 88  HCRSITFQHVPRSFNIVAHDLARTESLE 116
             R+I F+H PRS N +AH LA TE+L+
Sbjct: 82  RSRNIVFKHTPRSANALAHILA-TETLK 108

[Close]

BLAST of HG10017336 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 59.7 bits (143), Expect = 2.2e-05
Identity = 39/107 (36.45%), Postives = 55/107 (51.40%), Query Frame = 0


Query: 4    YGGVISTPTGCLVGAMHGFKNISLSPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVIQ 63
            +G +I   +  LV A        LSPL A+   I EGL+ A      H+ V SDSL+ IQ
Sbjct: 1317 FGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQ 1376

Query: 64   ILRGEVKNRVEVATYVADIQDMTVHCRSITFQHVPRSFNIVAHDLAR 111
            ++R E+  R +   +V +IQ +T     I+F H  R  N  AH LA+
Sbjct: 1377 LIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAK 1423

[Close]

BLAST of HG10017336 vs. NCBI nr
Match: XP_022158489.1 (uncharacterized protein LOC111024968 [Momordica charantia])

HSP 1 Score: 58.9 bits (141), Expect = 3.8e-05
Identity = 45/123 (36.59%), Postives = 65/123 (52.85%), Query Frame = 0


Query: 3   GYGGVISTPTGCLVGAMHGFKNISLSPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVI 62
           G G ++      +VGAM  F  +  +PL AK L I EGL LA R+ +  ++V +DSL  +
Sbjct: 89  GVGVLLREGNAEIVGAMVDFHMVK-TPLLAKILAIREGLSLATRLGVHRVVVETDSLEAL 148

Query: 63  QILRGEVKNRVEVATYVADIQDMTVHCRSITFQHVPRSFNIVAHDLAR-TESLENCALWC 122
            ++  +   + E  ++V DI+      + I FQHV R  N VA+ L R T SL    LW 
Sbjct: 149 NLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFRESNAVAYHLGRETISLRCGFLWR 208

Query: 123 DDF 125
            DF
Sbjct: 209 LDF 210

[Close]

BLAST of HG10017336 vs. ExPASy TrEMBL
Match: A0A5C7IFK3 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_009054 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 5.7e-07
Identity = 36/94 (38.30%), Postives = 55/94 (58.51%), Query Frame = 0


Query: 32  AKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTVHCRS 91
           A+A  IYEG+ LA    L  + + SDSL V+++  GE+  R +V   + DIQ +    R 
Sbjct: 187 AEAKAIYEGILLATTSGLQSLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 246

Query: 92  ITFQHVPRSFNIVAHDLARTE-SLENCALWCDDF 125
           I+  ++PRS N+VAH++AR    LEN  +  D +
Sbjct: 247 ISISYIPRSCNMVAHEVARRAIGLENSVIMYDPY 280

[Close]

BLAST of HG10017336 vs. ExPASy TrEMBL
Match: A0A5C7IST2 (RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 4.8e-06
Identity = 36/94 (38.30%), Postives = 53/94 (56.38%), Query Frame = 0


Query: 32  AKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTVHCRS 91
           A+A  IYEGL LA    L  + + SDSL V+++  GE+  R +V   + DIQ +    R 
Sbjct: 290 AEAKVIYEGLLLATSSGLQPLSIESDSLNVVRLCNGEISTRNDVFNVICDIQFLLSRERK 349

Query: 92  ITFQHVPRSFNIVAHDLARTE-SLENCALWCDDF 125
            +  ++PRS N VAH++AR    LEN  +  D +
Sbjct: 350 TSISYIPRSCNRVAHEVARRAIGLENSVIMYDPY 383

[Close]

BLAST of HG10017336 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-05
Identity = 39/107 (36.45%), Postives = 55/107 (51.40%), Query Frame = 0


Query: 4    YGGVISTPTGCLVGAMHGFKNISLSPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVIQ 63
            +G +I   +  LV A        LSPL A+   I EGL+ A      H+ V SDSL+ IQ
Sbjct: 1317 FGCIIRDSSCSLVAATSIRVPFPLSPLLAEIRCILEGLKFAAASNFTHLEVESDSLLAIQ 1376

Query: 64   ILRGEVKNRVEVATYVADIQDMTVHCRSITFQHVPRSFNIVAHDLAR 111
            ++R E+  R +   +V +IQ +T     I+F H  R  N  AH LA+
Sbjct: 1377 LIRNEIHTRGDEQNWVMEIQALTCCFAFISFSHSSRQCNRAAHGLAK 1423

[Close]

BLAST of HG10017336 vs. ExPASy TrEMBL
Match: A0A7J9GVN4 (RNase H domain-containing protein OS=Gossypium harknessii OX=34285 GN=Gohar_011989 PE=4 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.1e-05
Identity = 32/88 (36.36%), Postives = 53/88 (60.23%), Query Frame = 0


Query: 28  SPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTV 87
           S   A+ +   + +++ I  + P I++  DSL +I+  + + +NR +V  Y+ DIQ  T 
Sbjct: 22  SAFAAEVIACRKAVQIGIEKQWPTIIIEVDSLAIIKKCKNKSQNRSQVGVYIHDIQQRTG 81

Query: 88  HCRSITFQHVPRSFNIVAHDLARTESLE 116
             R+I F+H PRS N +AH LA TE+L+
Sbjct: 82  RSRNIVFKHTPRSANALAHILA-TETLK 108

[Close]

BLAST of HG10017336 vs. ExPASy TrEMBL
Match: A0A6J1DZK3 (uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024968 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.8e-05
Identity = 45/123 (36.59%), Postives = 65/123 (52.85%), Query Frame = 0


Query: 3   GYGGVISTPTGCLVGAMHGFKNISLSPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVI 62
           G G ++      +VGAM  F  +  +PL AK L I EGL LA R+ +  ++V +DSL  +
Sbjct: 89  GVGVLLREGNAEIVGAMVDFHMVK-TPLLAKILAIREGLSLATRLGVHRVVVETDSLEAL 148

Query: 63  QILRGEVKNRVEVATYVADIQDMTVHCRSITFQHVPRSFNIVAHDLAR-TESLENCALWC 122
            ++  +   + E  ++V DI+      + I FQHV R  N VA+ L R T SL    LW 
Sbjct: 149 NLIGDKSPWKGEAVSWVEDIRAFARDFQEICFQHVFRESNAVAYHLGRETISLRCGFLWR 208

Query: 123 DDF 125
            DF
Sbjct: 209 LDF 210

[Close]

BLAST of HG10017336 vs. TAIR 10
Match: AT1G10000.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 6.9e-05
Identity = 28/83 (33.73%), Postives = 45/83 (54.22%), Query Frame = 0


Query: 28  SPLGAKALTIYEGLRLAIRMELPHILVLSDSLIVIQILRGEVKNRVEVATYVADIQDMTV 87
           SPL A+A  I   +  A+++E   +LVLSDS  ++  L   V    E+   + +I+ +  
Sbjct: 203 SPLAAEAWAIKSAMLHALQLERSDLLVLSDSKSIVDALNSNVSLN-EIFGLLVEIRSIRN 262

Query: 88  HCRSITFQHVPRSFNIVAHDLAR 111
             RSI+FQ +PR  N +A   A+
Sbjct: 263 RFRSISFQFIPRLVNSIADAAAK 284

The following BLAST results are available for this feature:

BLAST of HG10017336 vs. NCBI nr


Match Name	E-value	Identity	Description
TXG67779.1	1.2e-06	38.30	hypothetical protein EZV62_009054 [Acer yangbiense]	[more]
TXG71894.1	1.0e-05	38.30	hypothetical protein EZV62_000473 [Acer yangbiense]	[more]
MBA0801636.1	2.2e-05	36.36	hypothetical protein [Gossypium harknessii]	[more]
XP_022158377.1	2.2e-05	36.45	uncharacterized protein LOC111024874 [Momordica charantia]	[more]
XP_022158489.1	3.8e-05	36.59	uncharacterized protein LOC111024968 [Momordica charantia]	[more]

BLAST of HG10017336 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of HG10017336 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5C7IFK3	5.7e-07	38.30	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_009054 ...	[more]
A0A5C7IST2	4.8e-06	38.30	RNase H domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_000473 ...	[more]
A0A6J1DX30	1.1e-05	36.45	uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]
A0A7J9GVN4	1.1e-05	36.36	RNase H domain-containing protein OS=Gossypium harknessii OX=34285 GN=Gohar_0119...	[more]
A0A6J1DZK3	1.8e-05	36.59	uncharacterized protein LOC111024968 OS=Momordica charantia OX=3673 GN=LOC111024...	[more]

BLAST of HG10017336 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10000.1	6.9e-05	33.73	Ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 3..110 e-value: 1.8E-19 score: 69.8
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 1..113 e-value: 1.0E-11 score: 47.0
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 2..125
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 2..125
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 2..110 e-value: 7.67988E-16 score: 66.5688
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 2..110

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10017336.1	HG10017336.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity