HG10017330 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10017330
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCASP-like protein
LocationChr03: 13155517 .. 13156829 (+)
RNA-Seq ExpressionHG10017330
SyntenyHG10017330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAACCCACAAGACAATAATGCCCCTAAATCAATCACGTCGGCTCCTCCTGCTCCCGACGTCGAAACAAGGCCGAGAATACCGCCGATTGGAGTAGAAGCAGCTGCAATAGTAAAGAAAATGAAGAGAGAAGACATGTTGAAGAAATTGTCTCTAGGTTTGAGATGGTCGAGTTTTGTATTCTCGTTTATTTCTTTTGTTGTCATGGTCTCCAATCGACATGGACAGGAGATTAATTTTGAAGATTATGAAGAATACAGGTATTGATATATAAACATATTTAATTTCTATTAAATTTAAGCTCTTTGAAATAAATTGTCAAGTCCACATATATGATAGAAGTGTTAAAAATATGAATTTATAATTAAAGTAACCGTAGGCTATCAACTTATCTAAATTTTTTAATTCGGTAGTGATTAATATATTTTTAGTTAAAAAAATAGCGAGAGTAAGAATTCAAGCACATCTTGATCTGACTTTTACCACAAATATATAATATCTTAATGAGTTAAGTTGTCACTTCGCTAATGATAAAATGGTTTGAAATGAACAGTTATTTGTTGGCAATTACAATAATCTCAAGTGTTTATACGGCCTACCAAGGAATTCGAGAAGTTATCCAATTTGTTACTAAAAAATCTACAATTCCTCAACTAGCTTTCGAAATCATCAACTTTGCTGTGGATCAGGTACAACTTACACTTTTTTTAGTACCACAATTGTAGGGATAAAAAATCGAACCTCCAACCTTATGAAATGAAATGCATACCAATTATTACTGACGTAAACTCAAATTGACATTTTTCTTTATTTAGTTTTCTCTGTTGGACTCCTTAAATATTAGGTTAAATATCAACATATTATATCTCTAAATTTTGTGAGTTGTATGAGTTAAAAACTCAAACTAATAATTGTATCTATCAATTGAAACTCTAAATGAAATCACCTAAATTAAATAAAATCATTCTTCTTCAAAAGTCTCATTAGAATAGCAATGAAAAAGCATCACGTAATTCTTTTTAAATAAGTTTGAGAAAAATATAGATATTTTTTTATTCTCTAATTCTCGTGCAACATTCTAACGGATTTCTGCGAAATTACAGATTTTGGCCTATTTATTGATATCAGCAGCATCAGCAGCTACTCCATTGACGAACAGAGGAAGAAAAGCTTACGAGGGATACTCAAATATAATGACATTTTTAGATATGGCGGCTGCTTCCATTAGCATGGCTTTCTTGGCCTTTTTTACCACAACAATCTCTCTTCTCATTTCTGGATACAAACTCTCTGCTCACTTTTTTCACTAA

mRNA sequence

ATGTCAAACCCACAAGACAATAATGCCCCTAAATCAATCACGTCGGCTCCTCCTGCTCCCGACGTCGAAACAAGGCCGAGAATACCGCCGATTGGAGTAGAAGCAGCTGCAATAGTAAAGAAAATGAAGAGAGAAGACATGTTGAAGAAATTGTCTCTAGGTTTGAGATGGTCGAGTTTTGTATTCTCGTTTATTTCTTTTGTTGTCATGGTCTCCAATCGACATGGACAGGAGATTAATTTTGAAGATTATGAAGAATACAGTTATTTGTTGGCAATTACAATAATCTCAAGTGTTTATACGGCCTACCAAGGAATTCGAGAAGTTATCCAATTTGTTACTAAAAAATCTACAATTCCTCAACTAGCTTTCGAAATCATCAACTTTGCTGTGGATCAGATTTTGGCCTATTTATTGATATCAGCAGCATCAGCAGCTACTCCATTGACGAACAGAGGAAGAAAAGCTTACGAGGGATACTCAAATATAATGACATTTTTAGATATGGCGGCTGCTTCCATTAGCATGGCTTTCTTGGCCTTTTTTACCACAACAATCTCTCTTCTCATTTCTGGATACAAACTCTCTGCTCACTTTTTTCACTAA

Coding sequence (CDS)

ATGTCAAACCCACAAGACAATAATGCCCCTAAATCAATCACGTCGGCTCCTCCTGCTCCCGACGTCGAAACAAGGCCGAGAATACCGCCGATTGGAGTAGAAGCAGCTGCAATAGTAAAGAAAATGAAGAGAGAAGACATGTTGAAGAAATTGTCTCTAGGTTTGAGATGGTCGAGTTTTGTATTCTCGTTTATTTCTTTTGTTGTCATGGTCTCCAATCGACATGGACAGGAGATTAATTTTGAAGATTATGAAGAATACAGTTATTTGTTGGCAATTACAATAATCTCAAGTGTTTATACGGCCTACCAAGGAATTCGAGAAGTTATCCAATTTGTTACTAAAAAATCTACAATTCCTCAACTAGCTTTCGAAATCATCAACTTTGCTGTGGATCAGATTTTGGCCTATTTATTGATATCAGCAGCATCAGCAGCTACTCCATTGACGAACAGAGGAAGAAAAGCTTACGAGGGATACTCAAATATAATGACATTTTTAGATATGGCGGCTGCTTCCATTAGCATGGCTTTCTTGGCCTTTTTTACCACAACAATCTCTCTTCTCATTTCTGGATACAAACTCTCTGCTCACTTTTTTCACTAA

Protein sequence

MSNPQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLAFFTTTISLLISGYKLSAHFFH
Homology
BLAST of HG10017330 vs. NCBI nr
Match: XP_038882342.1 (CASP-like protein 4B1 [Benincasa hispida])

HSP 1 Score: 317.4 bits (812), Expect = 8.9e-83
Identity = 166/200 (83.00%), Postives = 181/200 (90.50%), Query Frame = 0

Query: 1   MSNPQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSF 60
           MSNP+DNNAP++ITSAP APDVETR  +PP+G EAAAIVK+MKRED+LKKLSL LRWSSF
Sbjct: 1   MSNPRDNNAPETITSAPLAPDVETRSTVPPVGTEAAAIVKRMKREDILKKLSLSLRWSSF 60

Query: 61  VFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIP 120
           VFS I+FVVMVSNRHG+EINFEDYEEYSY+LAI IISSVYTAYQGIREVIQFVT+K T P
Sbjct: 61  VFSLIAFVVMVSNRHGEEINFEDYEEYSYVLAIAIISSVYTAYQGIREVIQFVTRKYTSP 120

Query: 121 QLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLA 180
            LAF II+F  DQ+LAYLLISAASAA PLTNR RK YEGYSN+MTFLDMAAASISM FLA
Sbjct: 121 HLAFAIIDFVADQVLAYLLISAASAAVPLTNRARKVYEGYSNVMTFLDMAAASISMTFLA 180

Query: 181 FFTTTISLLISGYKLSAHFF 201
           FFT  +SLLISGYKLSAHFF
Sbjct: 181 FFTLALSLLISGYKLSAHFF 200

BLAST of HG10017330 vs. NCBI nr
Match: XP_011654376.1 (CASP-like protein 4B1 [Cucumis sativus] >KGN54424.1 hypothetical protein Csa_021561 [Cucumis sativus])

HSP 1 Score: 262.7 bits (670), Expect = 2.6e-66
Identity = 147/201 (73.13%), Postives = 166/201 (82.59%), Query Frame = 0

Query: 1   MSN-PQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSS 60
           MSN PQ  N  +SITS P AP+VETRP+IP I ++AA +VKKMKRE+ML  LSL LRWSS
Sbjct: 1   MSNDPQVINVSESITSVPSAPNVETRPKIPSIEIDAAGVVKKMKRENMLSILSLSLRWSS 60

Query: 61  FVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTI 120
           FVFSF++FVVM+SNRHG+ INFE+YEEYSYLLAI IIS+VYTAYQGIREVIQFVTK  T 
Sbjct: 61  FVFSFLAFVVMLSNRHGEGINFEEYEEYSYLLAIAIISTVYTAYQGIREVIQFVTKIYTF 120

Query: 121 PQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKA-YEGYSNIMTFLDMAAASISMAF 180
           PQ  F II+F  DQ+LAYLLISAASAA PLTNRGRK   +G++ I  F DMAAASISMAF
Sbjct: 121 PQPTFAIIDFVADQVLAYLLISAASAAVPLTNRGRKLDDQGFTIITKFFDMAAASISMAF 180

Query: 181 LAFFTTTISLLISGYKLSAHF 200
           LA+FT  ISLLISGYK S HF
Sbjct: 181 LAYFTFAISLLISGYKFSTHF 201

BLAST of HG10017330 vs. NCBI nr
Match: XP_008464244.1 (PREDICTED: CASP-like protein 4B1 [Cucumis melo])

HSP 1 Score: 243.8 bits (621), Expect = 1.3e-60
Identity = 141/197 (71.57%), Postives = 158/197 (80.20%), Query Frame = 0

Query: 7   NNAP-KSITSAPPAPDVETRPRIPPIGVEA-AAIVKKMKREDMLKKLSLGLRWSSFVFSF 66
           N+ P +S TS P  P+VET    PPI +E+  A VKKMKRE+MLK LSL LRW SFVFSF
Sbjct: 3   NDVPAESTTSVPSTPNVET----PPIQIESTTATVKKMKRENMLKILSLSLRWLSFVFSF 62

Query: 67  ISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIPQLAF 126
           ++FVVM SNRHG+  NFED+EEYSYLLAI IIS VYTAYQGIREVIQFVTKK T PQ AF
Sbjct: 63  VAFVVMASNRHGERFNFEDFEEYSYLLAIAIISVVYTAYQGIREVIQFVTKKDTFPQPAF 122

Query: 127 EIINFAVDQILAYLLISAASAATPLTNRGRKAYE-GYSNIMTFLDMAAASISMAFLAFFT 186
            II+F  DQ+LAYLLISAASAA P TNRGRK  +  ++ I  F DMAAASISMAFLAFFT
Sbjct: 123 VIIDFVADQVLAYLLISAASAAVPSTNRGRKTDDLVFTIIKKFFDMAAASISMAFLAFFT 182

Query: 187 TTISLLISGYKLSAHFF 201
           + +SLLISGYKLSAHFF
Sbjct: 183 SALSLLISGYKLSAHFF 195

BLAST of HG10017330 vs. NCBI nr
Match: KAG6595570.1 (CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 219.9 bits (559), Expect = 1.9e-53
Identity = 133/220 (60.45%), Postives = 160/220 (72.73%), Query Frame = 0

Query: 1   MSNPQDNNAP---KSITSAPP---------APDVE---TRPRI-----PPIGVEAAAIVK 60
           MSNP + +     ++I+SAPP         +PDVE   T P +     P IGVE AAI+K
Sbjct: 1   MSNPNERSFTEILETISSAPPPLDVEKKSTSPDVEKKNTSPGVEKKSTPTIGVETAAIIK 60

Query: 61  KMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVY 120
           + KREDMLKK SL LRW  F+FSF++FV+M SNRHG+ +NFEDYEEYSYLLAI IISSVY
Sbjct: 61  RKKREDMLKKSSLVLRWLCFIFSFLAFVLMASNRHGRGLNFEDYEEYSYLLAIAIISSVY 120

Query: 121 TAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGY 180
           + YQGIREVIQ V +K T  +  F  I+F  DQILAYLLISAASAA P TN  R+  +  
Sbjct: 121 SVYQGIREVIQLVNRKPTFSRPVFATIDFMGDQILAYLLISAASAAVPRTNYARQNSDN- 180

Query: 181 SNIMTFLDMAAASISMAFLAFFTTTISLLISGYKLSAHFF 201
           S +M F+DMA+ASISMAFLAFFT  +SLLISGY+L+AHFF
Sbjct: 181 SMLMKFVDMASASISMAFLAFFTLALSLLISGYQLAAHFF 219

BLAST of HG10017330 vs. NCBI nr
Match: KAG7027551.1 (CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 217.6 bits (553), Expect = 9.6e-53
Identity = 132/220 (60.00%), Postives = 159/220 (72.27%), Query Frame = 0

Query: 1   MSNPQDNNAP---KSITSAPP---------APDVE---TRPRI-----PPIGVEAAAIVK 60
           MSNP + +     ++I+SAPP         +PDVE   T P +     P IGVE AAI+K
Sbjct: 1   MSNPNERSFTEILETISSAPPPLDVEKKSTSPDVEKKNTSPGVEKKSTPTIGVETAAIIK 60

Query: 61  KMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVY 120
           + KREDMLKK SL LRW  F+FSF++FV+  SNRHG+ +NFEDYEEYSYLLAI IISSVY
Sbjct: 61  RKKREDMLKKSSLVLRWLCFIFSFLAFVLTASNRHGRGLNFEDYEEYSYLLAIAIISSVY 120

Query: 121 TAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGY 180
           + YQGIREVIQ V +K T  +  F  I+F  DQILAYLLISAASAA P TN  R+  +  
Sbjct: 121 SVYQGIREVIQLVNRKPTFSRPVFATIDFMGDQILAYLLISAASAAVPRTNYARQNSDN- 180

Query: 181 SNIMTFLDMAAASISMAFLAFFTTTISLLISGYKLSAHFF 201
           S +M F+DMA+ASISMAFLAFFT  +SLLISGY+L+AHFF
Sbjct: 181 SMLMKFVDMASASISMAFLAFFTLALSLLISGYQLAAHFF 219

BLAST of HG10017330 vs. ExPASy Swiss-Prot
Match: D7LBN4 (CASP-like protein 4B1 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT_321547 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.8e-30
Identity = 90/200 (45.00%), Postives = 123/200 (61.50%), Query Frame = 0

Query: 1   MSNPQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSF 60
           M+NP D   P  +T    A +  + P  P  G   + I ++ KRED++KK S   R    
Sbjct: 1   MTNP-DKQKPVEVTDVETAAEKTSEP-TPASG--TSTITQRWKREDLIKKASPITRGICL 60

Query: 61  VFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIP 120
           +FS ++F++MVSN+HG   NF +YEEY Y+LAI+IIS++YTA+Q         +K+    
Sbjct: 61  LFSLLAFLIMVSNKHGYGRNFNEYEEYRYVLAISIISTLYTAWQ----TFAHFSKREFFD 120

Query: 121 QLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLA 180
           +    +++F+ DQI+AYLLISAAS+A PLTNR R   EG  NI  F D AA++ISMA  A
Sbjct: 121 RRTSTLVDFSGDQIVAYLLISAASSAIPLTNRFR---EGQDNI--FTDSAASAISMAIFA 180

Query: 181 FFTTTISLLISGYKLSAHFF 201
           F    +S L SGYKLS H F
Sbjct: 181 FVALALSALFSGYKLSTHSF 187

BLAST of HG10017330 vs. ExPASy Swiss-Prot
Match: Q8LE26 (CASP-like protein 4B1 OS=Arabidopsis thaliana OX=3702 GN=At2g38480 PE=2 SV=2)

HSP 1 Score: 133.3 bits (334), Expect = 3.1e-30
Identity = 92/200 (46.00%), Postives = 122/200 (61.00%), Query Frame = 0

Query: 1   MSNPQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSF 60
           M+NP DN  P   T    A +  + P  P  G   + I ++ KRED++KK S   R    
Sbjct: 1   MTNP-DNMKPVEATDVESAAEKTSEP-TPASG--TSTITQRWKREDLIKKASPITRGICL 60

Query: 61  VFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIP 120
           +FS I+F++MVSN+HG   NF DYEEY Y+LAI+IIS++YTA+Q         +K+    
Sbjct: 61  LFSLIAFLIMVSNKHGYGRNFNDYEEYRYVLAISIISTLYTAWQ----TFAHFSKREIFD 120

Query: 121 QLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLA 180
           +    +++F+ DQI+AYLLISAAS+A PLTN  R   EG  NI  F D AA++ISMA  A
Sbjct: 121 RRTSILVDFSGDQIVAYLLISAASSAIPLTNIFR---EGQDNI--FTDSAASAISMAIFA 180

Query: 181 FFTTTISLLISGYKLSAHFF 201
           F    +S L SGYKLS H F
Sbjct: 181 FIALALSALFSGYKLSTHSF 187

BLAST of HG10017330 vs. ExPASy Swiss-Prot
Match: Q10MR5 (CASP-like protein 4B2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0298300 PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.9e-27
Identity = 79/196 (40.31%), Postives = 115/196 (58.67%), Query Frame = 0

Query: 15  SAPPAPDVE----TRPRIPPIGVEAAA----------IVKKMKREDMLKKLSLGLRWSSF 74
           +A P PDVE    +     P    AAA          +V + +REDML K  L L  ++ 
Sbjct: 11  AAKPPPDVEKPDYSSQNGAPNSAAAAAGGGGGGVVDSVVARWRREDMLDKSPLALHAAAA 70

Query: 75  VFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIP 134
            F+F++ V++ SN+HG  + F+ Y+EY YLLAI  ++  Y+  Q +R  ++       +P
Sbjct: 71  AFAFVALVLVASNQHGDWMEFDRYQEYRYLLAIAALAFAYSLAQALRHALRMRRGVDPVP 130

Query: 135 QLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLA 194
             +  +++FA DQ++AYLL+SA SAATP+TNR R A      I  F D  AA+ISMAFLA
Sbjct: 131 TASGRLLDFASDQVVAYLLMSALSAATPITNRMRSAV-----INRFTDTTAAAISMAFLA 190

Query: 195 FFTTTISLLISGYKLS 197
           F +  +S ++SGYKLS
Sbjct: 191 FVSLALSAIVSGYKLS 201

BLAST of HG10017330 vs. ExPASy Swiss-Prot
Match: F2D276 (CASP-like protein 4B2 OS=Hordeum vulgare subsp. vulgare OX=112509 PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.0e-25
Identity = 68/164 (41.46%), Postives = 99/164 (60.37%), Query Frame = 0

Query: 37  AIVKKMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITII 96
           ++V + +REDML K  L L  ++  F+F++ V++ SN+HG  + F+ Y+EY YLLAI  +
Sbjct: 44  SVVARWRREDMLDKCPLALHAAAAAFAFVALVLVASNQHGDWMQFDRYQEYMYLLAIAAL 103

Query: 97  SSVYTAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKA 156
           +  Y+  Q +R   +       IP  +  + +F  DQ++AYLL+SA SAA P+TNR R A
Sbjct: 104 AFAYSLAQALRHAHRMRGGADPIPAPSARLFDFIADQVVAYLLMSALSAAIPITNRMRTA 163

Query: 157 YEGYSNIMTFLDMAAASISMAFLAFFTTTISLLISGYKLSAHFF 201
                 I  F D  AA+ISMAFLAF    +S  +SGYKLS   +
Sbjct: 164 V-----INNFTDATAAAISMAFLAFVALALSATVSGYKLSRQMY 202

BLAST of HG10017330 vs. ExPASy Swiss-Prot
Match: Q5W6M3 (CASP-like protein 4B4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0344400 PE=3 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.8e-25
Identity = 78/204 (38.24%), Postives = 120/204 (58.82%), Query Frame = 0

Query: 8   NAPKSITSAPPAPDVETRPRIPPIGVE---------------AAAIVKKMKREDMLKKLS 67
           +A  + +S  PA DVE         V+                +A+V++ +R+D+L+K  
Sbjct: 2   SAAVAASSGAPAADVEKGAAAADANVDGGGAPAAAAASGEGVVSAVVRRWRRQDLLEKSG 61

Query: 68  LGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQF 127
             LR +++ FS ++FVVM +N HG    FE YEEY Y++AI +++ +YT  Q +R  ++ 
Sbjct: 62  SALRVAAWAFSLLAFVVMGANDHGDWRQFEHYEEYRYVVAIGVLAFIYTTLQLVRHGVRL 121

Query: 128 VTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAA 187
              +    ++A  +++FA DQ+ AYLL+SA SAA P+TNR R   EG  N+  F D +AA
Sbjct: 122 TGGQDLQGKVAV-LVDFAGDQVTAYLLMSAVSAAIPITNRMR---EGADNV--FTDSSAA 181

Query: 188 SISMAFLAFFTTTISLLISGYKLS 197
           SISMAF AF    +S L+SG+KL+
Sbjct: 182 SISMAFFAFLCLALSALVSGFKLA 199

BLAST of HG10017330 vs. ExPASy TrEMBL
Match: A0A0A0L029 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_4G314510 PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 1.3e-66
Identity = 147/201 (73.13%), Postives = 166/201 (82.59%), Query Frame = 0

Query: 1   MSN-PQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSS 60
           MSN PQ  N  +SITS P AP+VETRP+IP I ++AA +VKKMKRE+ML  LSL LRWSS
Sbjct: 1   MSNDPQVINVSESITSVPSAPNVETRPKIPSIEIDAAGVVKKMKRENMLSILSLSLRWSS 60

Query: 61  FVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTI 120
           FVFSF++FVVM+SNRHG+ INFE+YEEYSYLLAI IIS+VYTAYQGIREVIQFVTK  T 
Sbjct: 61  FVFSFLAFVVMLSNRHGEGINFEEYEEYSYLLAIAIISTVYTAYQGIREVIQFVTKIYTF 120

Query: 121 PQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKA-YEGYSNIMTFLDMAAASISMAF 180
           PQ  F II+F  DQ+LAYLLISAASAA PLTNRGRK   +G++ I  F DMAAASISMAF
Sbjct: 121 PQPTFAIIDFVADQVLAYLLISAASAAVPLTNRGRKLDDQGFTIITKFFDMAAASISMAF 180

Query: 181 LAFFTTTISLLISGYKLSAHF 200
           LA+FT  ISLLISGYK S HF
Sbjct: 181 LAYFTFAISLLISGYKFSTHF 201

BLAST of HG10017330 vs. ExPASy TrEMBL
Match: A0A1S3CLH8 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103502174 PE=3 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 6.1e-61
Identity = 141/197 (71.57%), Postives = 158/197 (80.20%), Query Frame = 0

Query: 7   NNAP-KSITSAPPAPDVETRPRIPPIGVEA-AAIVKKMKREDMLKKLSLGLRWSSFVFSF 66
           N+ P +S TS P  P+VET    PPI +E+  A VKKMKRE+MLK LSL LRW SFVFSF
Sbjct: 3   NDVPAESTTSVPSTPNVET----PPIQIESTTATVKKMKRENMLKILSLSLRWLSFVFSF 62

Query: 67  ISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIPQLAF 126
           ++FVVM SNRHG+  NFED+EEYSYLLAI IIS VYTAYQGIREVIQFVTKK T PQ AF
Sbjct: 63  VAFVVMASNRHGERFNFEDFEEYSYLLAIAIISVVYTAYQGIREVIQFVTKKDTFPQPAF 122

Query: 127 EIINFAVDQILAYLLISAASAATPLTNRGRKAYE-GYSNIMTFLDMAAASISMAFLAFFT 186
            II+F  DQ+LAYLLISAASAA P TNRGRK  +  ++ I  F DMAAASISMAFLAFFT
Sbjct: 123 VIIDFVADQVLAYLLISAASAAVPSTNRGRKTDDLVFTIIKKFFDMAAASISMAFLAFFT 182

Query: 187 TTISLLISGYKLSAHFF 201
           + +SLLISGYKLSAHFF
Sbjct: 183 SALSLLISGYKLSAHFF 195

BLAST of HG10017330 vs. ExPASy TrEMBL
Match: A0A6J1EB75 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111432533 PE=3 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 3.0e-52
Identity = 130/221 (58.82%), Postives = 159/221 (71.95%), Query Frame = 0

Query: 1   MSNPQDNNAP---KSITSAPP----------APDVE---TRPRI-----PPIGVEAAAIV 60
           MSNP + +     ++I+SAPP          +PDVE   T P +     P IGVE AAI+
Sbjct: 1   MSNPNERSFTEILETISSAPPPPLDVEKKSTSPDVEKKNTSPDVEKKSTPTIGVETAAII 60

Query: 61  KKMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSV 120
           K+ KRED LKK SL LRW  F+FSF++FV+M SNRHG+ +NFEDYEEYSYL+AI IISSV
Sbjct: 61  KRKKREDTLKKSSLVLRWLCFIFSFLAFVLMASNRHGRGLNFEDYEEYSYLVAIAIISSV 120

Query: 121 YTAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEG 180
           Y+ YQGIREVIQ V +K T  +  F  I+F  DQILAYLLISAASAA P TN  R+  + 
Sbjct: 121 YSVYQGIREVIQLVNRKPTFSRPVFATIDFIGDQILAYLLISAASAAVPRTNYARQNSDN 180

Query: 181 YSNIMTFLDMAAASISMAFLAFFTTTISLLISGYKLSAHFF 201
            S +M F+DMA+ASISMAFLAFFT  +S+LISGY+L+AHFF
Sbjct: 181 -SMLMKFVDMASASISMAFLAFFTLALSVLISGYQLAAHFF 220

BLAST of HG10017330 vs. ExPASy TrEMBL
Match: A0A6J1HPJ6 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111466168 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 5.2e-52
Identity = 128/213 (60.09%), Postives = 150/213 (70.42%), Query Frame = 0

Query: 1   MSNPQDNNAPK-----SITSAPPAPDVETRPRIP--------PIGVEAAAIVKKMKREDM 60
           MSNP + +  +     S T  PP PDVE +   P         IGVE AAI+K+ KRED 
Sbjct: 1   MSNPNEKSFTEILETISSTPPPPPPDVEKKSTSPDVEKKSTSTIGVETAAIIKRKKREDT 60

Query: 61  LKKLSLGLRWSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIR 120
           LKK  L LRW  F+FSF++FV+M SNRHG+ +NFEDYEEYSYLLAI IISSVY+ YQGIR
Sbjct: 61  LKKSYLVLRWLCFIFSFLAFVLMASNRHGRGLNFEDYEEYSYLLAIAIISSVYSVYQGIR 120

Query: 121 EVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFL 180
           EVIQ V +K T  +  F II+F  DQILAYLLISAASAA P TN  R      S +M F+
Sbjct: 121 EVIQLVNRKPTFSRPVFVIIDFIGDQILAYLLISAASAAVPRTNYARH-NSNNSMLMKFV 180

Query: 181 DMAAASISMAFLAFFTTTISLLISGYKLSAHFF 201
           DMA+ASISMAFLAFFT  +S+LISGY+L AHFF
Sbjct: 181 DMASASISMAFLAFFTLALSVLISGYQLFAHFF 212

BLAST of HG10017330 vs. ExPASy TrEMBL
Match: A0A7J7P4J1 (CASP-like protein OS=Kingdonia uniflora OX=39325 GN=GIB67_020270 PE=3 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.7e-34
Identity = 97/200 (48.50%), Postives = 131/200 (65.50%), Query Frame = 0

Query: 1   MSNPQDNNAPKSITSAPPA----PDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLR 60
           M+  +D  A K + S PPA    P V+   + P  GV  ++IV++ +RED+LKK SL LR
Sbjct: 1   MTTTEDPKATKPVLSPPPATPVVPPVDVERQAPAFGV--SSIVRRWRREDLLKKGSLVLR 60

Query: 61  WSSFVFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKK 120
               +FS +SF++M SNRHG   NF+ YEEY YLL I +++++YTA Q IR++ QF + K
Sbjct: 61  AVGLLFSVLSFIIMASNRHGDWKNFDKYEEYRYLLCIALLATIYTAAQVIRQMHQFSSGK 120

Query: 121 STIPQLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISM 180
           +   Q    +++F  DQ++AYLLISAAS A PLTNR R   EG  NI  F D  AASISM
Sbjct: 121 AMFSQHTSSLVDFFGDQVVAYLLISAASTAVPLTNRMR---EGSDNI--FTDSTAASISM 180

Query: 181 AFLAFFTTTISLLISGYKLS 197
           AF AF T  +S +ISG+KL+
Sbjct: 181 AFFAFVTLALSAMISGFKLA 193

BLAST of HG10017330 vs. TAIR 10
Match: AT2G38480.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 133.3 bits (334), Expect = 2.2e-31
Identity = 92/200 (46.00%), Postives = 122/200 (61.00%), Query Frame = 0

Query: 1   MSNPQDNNAPKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSF 60
           M+NP DN  P   T    A +  + P  P  G   + I ++ KRED++KK S   R    
Sbjct: 1   MTNP-DNMKPVEATDVESAAEKTSEP-TPASG--TSTITQRWKREDLIKKASPITRGICL 60

Query: 61  VFSFISFVVMVSNRHGQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIP 120
           +FS I+F++MVSN+HG   NF DYEEY Y+LAI+IIS++YTA+Q         +K+    
Sbjct: 61  LFSLIAFLIMVSNKHGYGRNFNDYEEYRYVLAISIISTLYTAWQ----TFAHFSKREIFD 120

Query: 121 QLAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLA 180
           +    +++F+ DQI+AYLLISAAS+A PLTN  R   EG  NI  F D AA++ISMA  A
Sbjct: 121 RRTSILVDFSGDQIVAYLLISAASSAIPLTNIFR---EGQDNI--FTDSAASAISMAIFA 180

Query: 181 FFTTTISLLISGYKLSAHFF 201
           F    +S L SGYKLS H F
Sbjct: 181 FIALALSALFSGYKLSTHSF 187

BLAST of HG10017330 vs. TAIR 10
Match: AT2G36330.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 87.4 bits (215), Expect = 1.4e-17
Identity = 57/167 (34.13%), Postives = 97/167 (58.08%), Query Frame = 0

Query: 36  AAIVKKMKREDMLKKLSLGLRWSSFVFSFISFVVMVSN--RHGQEINFEDYEEYSYLLAI 95
           +AI+++ +RE+++K  +LG R S  V + ISF +M ++  +     +F+ Y+EY + L++
Sbjct: 120 SAILRRSRREEVVKFSALGFRLSEVVLALISFSIMAADKTKGWSGDSFDRYKEYRFCLSV 179

Query: 96  TIISSVYTAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLISAASAATP----- 155
            +++ VY+++Q        V +K  I      +  F +DQ+LAYLL+SA++AA       
Sbjct: 180 NVVAFVYSSFQACDLAYHLVKEKHLISHHLRPLFEFIIDQVLAYLLMSASTAAVTRVDDW 239

Query: 156 LTNRGRKAYEGYSNIMTFLDMAAASISMAFLAFFTTTISLLISGYKL 196
           ++N G+           F +MA+ASI+M+FLAF     S LISGY L
Sbjct: 240 VSNWGKD---------EFTEMASASIAMSFLAFLAFAFSSLISGYNL 277

BLAST of HG10017330 vs. TAIR 10
Match: AT5G62820.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 74.7 bits (182), Expect = 9.4e-14
Identity = 58/185 (31.35%), Postives = 101/185 (54.59%), Query Frame = 0

Query: 24  TRPRIPPIGVEAA--AIVKKMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNR--HGQEI 83
           TR    PI  E +    V + + +D++   +LG R +  +   ISF +M +++       
Sbjct: 120 TRKAGAPINGEESTRTTVNRARGDDLVSLTALGFRITEVILCVISFSIMAADKTQGWSGD 179

Query: 84  NFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIPQLAF-EIINFAVDQILAYL 143
           +++ Y+EY Y LA+ +I+ VY+A++   +   ++ K+S +    F ++  F++DQ+LAYL
Sbjct: 180 SYDRYKEYRYCLAVNVIAFVYSAFEAC-DAACYIAKESYMINCGFHDLFVFSMDQLLAYL 239

Query: 144 LISAASAATP-----LTNRGRKAYEGYSNIMTFLDMAAASISMAFLAFFTTTISLLISGY 199
           L+SA+S A       ++N G+           F  MA ASI+++FLAF    +S LIS Y
Sbjct: 240 LMSASSCAATRVDDWVSNWGKD---------EFTQMATASIAVSFLAFGAFAVSALISSY 294

BLAST of HG10017330 vs. TAIR 10
Match: AT5G40300.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 61.6 bits (148), Expect = 8.2e-10
Identity = 50/177 (28.25%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 23  ETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSFVFSFISFVVMVSNRH--GQEIN 82
           E+R ++ P    +++  ++ K   +++K  LG R  +FV   +SF VMVS+R       +
Sbjct: 94  ESRRQLRPSFSSSSSTPRESKWASLIRKALLGFRVIAFVSCLVSFSVMVSDRDKGWAHDS 153

Query: 83  FEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIPQLAFEIINFAVDQILAYLLI 142
           F +Y+E+ + LA  +I  VY+ +     V    T            + F +DQ+LAYLL 
Sbjct: 154 FYNYKEFRFCLAANVIGFVYSGFMICDLVYLLSTSIRRSRHNLRHFLEFGLDQMLAYLLA 213

Query: 143 SAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLAFFTTTISLLISGYKLSA 198
           SA+++A+   +     ++       F D+A AS+++++++F       L SGY L A
Sbjct: 214 SASTSASIRVD----DWQSNWGADKFPDLARASVALSYVSFVAFAFCSLASGYALCA 266

BLAST of HG10017330 vs. TAIR 10
Match: AT4G11655.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 57.8 bits (138), Expect = 1.2e-08
Identity = 55/198 (27.78%), Postives = 88/198 (44.44%), Query Frame = 0

Query: 10  PKSITSAPPAPDVETRPRIPPIGVEAAAIVKKMKREDMLKKLSLGLRWSSFVFSFISFVV 69
           P S  S   +P  +  P +    V  +A  +   R   +  + L LR+ + V  F+S + 
Sbjct: 15  PSSEASVTASPVSQQTPSLFAYSVTPSA-SRFSSRRASVHVIGLVLRFITMVLCFVSALS 74

Query: 70  MV------SNRH--GQEINFEDYEEYSYLLAITIISSVYTAYQGIREVIQFVTKKSTIPQ 129
           +       S RH      +F  Y E  Y   + +I  VYT+ Q  + V     +   I +
Sbjct: 75  LAVNVQRPSKRHLTQNSSSFASYPELLYCFGVAVIGFVYTSLQTFKGVCDITHRGVLISE 134

Query: 130 LAFEIINFAVDQILAYLLISAASAATPLTNRGRKAYEGYSNIMTFLDMAAASISMAFLAF 189
              + I+F  DQ++ YLL+S++S A          +     I T  + +  S+SM+F AF
Sbjct: 135 PLSDYISFIFDQVICYLLVSSSSVAIAWIQ-----HINEDAIKTLRNNSIVSVSMSFSAF 194

Query: 190 FTTTISLLISGYKLSAHF 200
              T+S L+SGYKL   F
Sbjct: 195 LVLTLSGLLSGYKLCKRF 206

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882342.18.9e-8383.00CASP-like protein 4B1 [Benincasa hispida][more]
XP_011654376.12.6e-6673.13CASP-like protein 4B1 [Cucumis sativus] >KGN54424.1 hypothetical protein Csa_021... [more]
XP_008464244.11.3e-6071.57PREDICTED: CASP-like protein 4B1 [Cucumis melo][more]
KAG6595570.11.9e-5360.45CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7027551.19.6e-5360.00CASP-like protein 4B1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
D7LBN41.8e-3045.00CASP-like protein 4B1 OS=Arabidopsis lyrata subsp. lyrata OX=81972 GN=ARALYDRAFT... [more]
Q8LE263.1e-3046.00CASP-like protein 4B1 OS=Arabidopsis thaliana OX=3702 GN=At2g38480 PE=2 SV=2[more]
Q10MR51.9e-2740.31CASP-like protein 4B2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0298300 P... [more]
F2D2761.0e-2541.46CASP-like protein 4B2 OS=Hordeum vulgare subsp. vulgare OX=112509 PE=2 SV=1[more]
Q5W6M31.8e-2538.24CASP-like protein 4B4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0344400 P... [more]
Match NameE-valueIdentityDescription
A0A0A0L0291.3e-6673.13CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_4G314510 PE=3 SV=1[more]
A0A1S3CLH86.1e-6171.57CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103502174 PE=3 SV=1[more]
A0A6J1EB753.0e-5258.82CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111432533 PE=3 SV=1[more]
A0A6J1HPJ65.2e-5260.09CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111466168 PE=3 SV=1[more]
A0A7J7P4J11.7e-3448.50CASP-like protein OS=Kingdonia uniflora OX=39325 GN=GIB67_020270 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G38480.12.2e-3146.00Uncharacterised protein family (UPF0497) [more]
AT2G36330.11.4e-1734.13Uncharacterised protein family (UPF0497) [more]
AT5G62820.19.4e-1431.35Uncharacterised protein family (UPF0497) [more]
AT5G40300.18.2e-1028.25Uncharacterised protein family (UPF0497) [more]
AT4G11655.11.2e-0827.78Uncharacterised protein family (UPF0497) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006702Casparian strip membrane protein domainPFAMPF04535DUF588coord: 47..182
e-value: 1.3E-28
score: 99.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..27
NoneNo IPR availablePANTHERPTHR11615:SF144CASP-LIKE PROTEIN 4B1coord: 20..199
NoneNo IPR availablePANTHERPTHR11615NITRATE, FORMATE, IRON DEHYDROGENASEcoord: 20..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10017330.1HG10017330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding