HG10017032 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10017032
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionGlutamine synthetase
LocationChr03: 10424099 .. 10431238 (+)
RNA-Seq ExpressionHG10017032
SyntenyHG10017032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCGAGTATTTGTGAGAATGGAGTTGATATCGGAGGCGATGCCAACAATCTGGGAGATGTTGTGCAAAAGGATACCAAGCTTGTTCGGATTATTTGGGTTGATGCTTCAGGACTCCATAGATGTCGTGTGAGTTTTTTTTACAAAAAAAAACAAAATATGTTTGAAAAATTACTTTTTGATCTCTTGATTTTGACTTTTAGATCTAGTTTTTCAATTGGCCATTGGGTTTCAAAAGGTTACTTTTTTAGTTTTTATGTTTTGAGTTTGATTTCAATTTAGTCCACTAATTTTGAAATATTTATAATTTTATCTTTAAAATCTGTTTTTTTTTTTTCAATTTAGTCCACTAATTTTAAAATATTTATAATTTTATCTTTAATTTTTTTTTTTTTTAATTTGGCCCTTAGGTTTCAAGATTTAAACTTTTGACTTTGATTTTTTTTTTTTTACTAAATAATCACTTTCAATTTAAGAGAATTTTTAAAAATAGAAAAATAAGAAAAACTATTTACACAAAATAGCAAAATTTTTAGATAGTTGTGGTAGATGTTGATAGAAGTCTATCAGGGTCTATCAGTGATAGAAAATTAGAAATGATAAAACTCTATTGCTGATAGATGCTGATAAAAGTCTATCAATGTCTATCAGTGTTTTTTTTTGCTATTTTCTATAAATAGTTTAACATTTTTTCTATCTATGAAATATTCTCTTCAATTTATAGTATTAATGTCTATTAATTAATTATATGTGTGATGGAGGATCGAACCTCTGACTTTGAGGTTGATAATACAATTTATGTCAATTAAATAACTTAAATTATGCCATGTTGATAAGTAAAATTAATTTTCACTATTTTTTCGTCGTTATTAAAATAAATTTTAAATGTTCACTTCATAATTATTTTAAATTAATTAATAGACATTAACGCCAAAAACAAAGTGAGTAGTTAGTCAAAAATTAAATTTTAAAAGTGTAAATTAATCTAGAAACTTTGGGACCAAATTGAAACAAAACGCAAATCTCAAGGCTAACATTTTAATGTTTTAAAACCTACGACTAAATTGAAATCGAACTCAAAACTTAATGACTAAAAATATACCATTTTCAAATTTAGGGAGAAAATGAAAATGAAACCCGAAACATAGGGATTGTTCATTTATTTATTTATTACAGTACAAACATTATACTAGTTAGTTGTGCTATGCTTATATAAATTGGCAACTTGAAAAATACTTGAGTGCATAATAGGTTGCACTCATTATTTTGCAGTCATTATTTTGCAGTGTGCCTATCGTTCAATCATAGTTTGCCACTTGTCTTCTAAATATTTCAAAGTGTATTTCGTCTATAATTAGTTGAGGTGCAACTGCAAAGAAATGTGCACAACCTAAGATGCAAACAATAGTTATTGGACATACTATCTCGCACTAATTGAGATTGTACTTACATAATTATCATCTTTTGAGAATAATGTGAGGTCCCAATTTTAAGTAATTTCGTATGAGAGGAAAAATTATAGTATACTTAGACATAGGAATTAGGTTTTACGAGAATACAAAAAATTTCTCGAATATCTTAACATACACGTAAAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAAATATGAGGTAGGTTGAGAGAATCGAATCTCGAAACTCGAAGTCGATAGTACAAATTTTATTATAGTTGAATTATGCTCGTTTTGACTATTCTTTTCGTCGTTTATGTCAATGGGAACAAAGTTTCAAAATTGTATAATTATTGTTGTAATTTTTTTAATCTTTATTTGATTAATTTATGTTGTAATGATTTCAGATTATTCCCTCTATGCGGTTCAATGATGTGGTTAAAAAGAGTGGGATTGGTTTATCTGTTTCATGTATGGCAAGGCCTTCTTTTAGCGATATTCCAGCGGGGCGTAATCTTACTGGTGTTGGTGAGATGAGACTGCTACCAGATTTATCAACCAAACGGACGGTTCCTTGGTATAAACTTTCCACCACAATGTTTTAAAATTCTTCTTGTATTTTAGTATTTCAATGGTACTTACGTCGTCATCAAGATTATTTGATGAGGCTGCGACAATGCGTGGTCGGTTGTTTTTTATCATTGGATTATGATAGAGAGTTATCTTAAATTTATATCAAGTGATTATTTGACGTCAAACGATTATTGTTGTGTAGTAACATTACCGTTGAATAATTTGTCATTAACTTGGCCAACCTAATGATCTATTTTTGAATTTCTCGAATTGCTTGGTAGTGGGCATATAATTAGTCATACCATAAGATGAGGAACGTAGATATCAATTAAGTGTCTGTTTGATAGGAAACTTGAAAATAAGAGATTCAAGGAAAACAAATTTGTATTTCATGTTTTTAGATATGTATTTGGTAGAAGATTCATATATTATATTTAAATTTAAACAATTATTCAAAATGTGTTTGATAACACACTTATAAACTATAGAACTAACTGTAGTTATCTACTAACTATTAAGTTGAATATAAACTATGATTAATTAATTTATGAACATATTTGATGTTAAAGTTTGTTTATAATTATTATTTTGTAATAGCATATAATTTATAATACATTATATAATACATATTTTAATTAACTAAAATAATTAAATTTATAACCTGAAATACTATATTTAATAACCTTTGCAATTTTGTTTTTTTTTTATTTTATATAATTATGTATTTAAAAATTTCAAATTCAAAATATTAAAACAGTTAAAACATTCAATTTTATTTAAAATGTTTTCAAAATTTAGACGATTTGGATCATAGAACACTTACAATTTTAAAAAATAAATCCAAATTATCTATCAAATGCATATTCGATAAATTTAGTGAATTTGAAAACATAAAATAGAATTCAAATTGCCCACCAAACAAGTCCAAAATTTTTTTTAGTGTGAATTTGAAATAGTTAAAATCATTTTATCGATCATTCCAAATCAATTTAATATTTGATTTTACATTTTTTTTTATACTTGACTTTCATACCATTAAAATTAATTTTAAATTATTAAAAGTATATATTTCAAAGCAATTTTGAGCCTGACTGAAGAAATGTATTCCCTTGCTTTGTTGGACAGGAGGGAGCAAGAGGAAATGGTAATGGGTGACATGTATATTAGACCTGGTGAATCATGGGAATACTGTCCAAGGGAAGCCTTACGTAGGGTCTCTAGAATTCTGAAAAATGAATTTGACTTGGTATGGCAAATTAATTATATACTGTTCAATTATTATTATTATATTTTTTTTATCAATGCTTATTATTTATTAGATATTCAGATTATAATGTTCAAGCCTTTTTTTTTCTCAATACTTACATTAGATCTTCAAAATTATTTATTTACTCTGTTATATGTCTTGTGTTGTGTCTAGGAAATGAAAGCAGGCTTTGAAATCGAGTTTTTGCTATTAAAAAAGGCAGTCAAGTAAGGATATATATATATATATATTTTTTTTTTTGTTAAATGCTCTTCTTTTCTTTATGTTGACCTTCATTTATTTTTGATTTTTTCAAATGGTCAATAAGTTCTATCTCTATTTAAATTGTCATAGAGATGGAAAAGAAGATTGGGTGCCATTTGATTCATCATTCTATTGTTCTGCATCTTCATATGATGCTGTCTCTCCCTTTTTTCATGACGTAGTTGCTTCTTTGAACTCCTTGAATATTATTGTGGAACAGGTATGAATTATAGAATTAAGAAAAAGAAATAAACTTACCTAATATTTATTCAAGTTTTACTCTATTATTATTATTATTATTATTATTTTTTGGATTAGAGAGGCTTTTATAAATGAAAAAAAAATTAATAAAATGCTAAAAAAACAAAACATTGCTTTTACTTCTTTAATCCCTAGATGGGATGATAAGTTATAGATTTTTTTAAATGTATATAAATGCTAGATACTTATTATTATTATTGAAAAAAAAGAAATTATTTATGGGGGACTTTTCTTATCTTATATAGTTGCATGCAGAGGTTGGAAATGGTCAATTTGAGTTTGCTTTAGGGTATACTTCTTGTCTCAGTGCTGCTGACGAATTAATTTACACTCGTGAAGTGATTAAGGCTATTGCAAGAAAGCATGGATTGTTGGCAACATTTATACCCAAGTATGTTTTACAATTCAATCAAGTCAAATCGCCATTAGCTTCTTGAATAAAAATATAATAATAGCAGTTTTTGTCTTCGATTTGTTAACGCACCGTTGGATAATATTTTCGTTTCTTGTTTTTAGTTTAGGTACACCAAAGATAATAGGTATAATTAAATTTGATATTCTAAAAGAAAAATATTTTTATAGACTTATTTGCACCTATCTTTTTTCGTCTATTAGTTGTCTAATCATAATTTGTCACGTGATAAATTTTTTTTGAAAAATTATTATAAACAGAAAAAATATGAAACTATTTACAAACACAGAAAAATTTCACTGTCTATCATCGATAAACCACGATAGAATTCTATCACTTAAGCAATAGAAAGAGATAAATTTCTATCGTTCAAGCTATAGAATTCTATTACAGTCTATTGCTGATAGACATTAAAATTTTTCTACATTTGTAGATAGTTTGACTCATTTTTTTACATTTAAAAAGAGTAATACTTGGGTTCACTCACATAAAAAAATTATTAAAATATTTTAAAAAAGAAACAAGAAGAATTTGTCACATGTCAAATTATGATGAGACAATTTGGTGGGATGCACAAAGATAATGCACCTAAGTCTTTTACATTTAAAAATAGTCCATCTTTTTTCTTATTTTATAGTTTTTGTTATCGTGTAATTAAAACTGACTATAAAAAGACGAGTGTACATAAATTAGAATAGACAAAGCATTTCTATGTACTAAAAGAAAAATACTTACGTACATCTCGAACTGCAATGTTGTGATTTGTTATGATTGTTTAGGTATGCAATGGATGACATGGGTTCTGGTTCCCACGTGCATATCAGTCTGTGGCGGAATGGAAAAAATGTTCTGATGGCAGCTGACAAATCTTCTAAGCATGGGATGTCAACAATTGGTGAAGAGTTCATGGCTGGCGTTTTCAATCATATTACATCAATTTTGCCTTTTATAGCTCCAGTTCCCAACAGGTGAGAACTTAGAACACCACCAATTCCATATTTTCTTTTAATAATTCCATGATTAGAATTTATTAGGCTCATTGGCCAATCAATTATCTGCTACATTGTTTGATGATTCTTGTAGTTATGACCGTATACAACCCAATACATGGTCTGGAGCTTATCATTGTTGGGGTAAAGATAACAAAGAAGCTCCAATTAGAACAGCCAGTCCACCTGGAATTGGTGTTAGTTTAGTGAGTAATTTTGAGGTCAAATCATTTGACGGTTGTGCAAACCCATATTTGGGTCTAGCTGCTATCCTTTGTGCTGGATTAGATGGCCTTCGTAACCATCTTCAGTTGCCTGAACCTATCGGTAACTTTCTTGTTCAATCTATTTGTGTTACTATTTACTTATAATGTGTAGATGGAATATTCGTGACACAACTATTCAAAACTGAAATAAAGGTAATCTAACGCACAATGACACTTGTTGCTGATAATGTTATAAAAATTCAAAGCAAATCAGATAAAAACATAGAAAGAATTAAGTAACTAAGTAAGAACAAGAACAAACCACAAGTTTATTTGGTAATCCAATTTGGTGCAACTTCATCTATGTCTAGGGGGAGGCGTGTCTGGGAAAAATAACTTAACTGATTATATAATGTAAAGTGATTGCAAATGATGTACTTATCTGTAAATAAATGGTTATTATAGTGACACGTGTAGAGGTTAACTCCTTAAATGATCATCTAAGCTCCCCTTAGATGTAAAACTCCCTCACAATGAAATTTAGGCTCCCTCTCAAAATACCTTAGGCTCTCCTTAAAGTGTGAGAACTCCCTCTTACTAAGACTTTATATTTCCACCGTCAAATGAAGACCACTTCACTCGATGAACTTAGGCTCCTCCTAAGTTTAAAAATCTCACTCGATGAACGCAATGATAACATCTTGATCTATAGCAACACAATCTTTACAATGGAAAGATTGACACACTTGTGATTTCTTTCGACACCACGAGAATGACTCCAACATTCTCTTGGAACGGTCAACCCTAAACTGAATGTCGCAACTCCTTAATTAAGCACAGTGGAAAAAAGGCAAGGTGACAACCCTTAAACATATTCGACAACGCTAAGAAAAGGAAACAAAATCGGAAGAATAAATGGCTTAGGATTTTCTAGATAAAGAAAAAAAAATATTATGCAGAATCAGAAATTTAACTAACAACAATTGAAACAACATTCTATCTCTAACAAAAACAATAAGATTATTTTTGGCTTGAGTAATGATATGGATTGAAAAACATTACTGGTTATCTCCCTCAATATTTTAATAAAATACAATTCTCTTTTTATTGAATCTTTTGTTTTCAAATTTTTACTTTTAAATACCCTATTAAACAGACTTTAAGCTACCCCTATGTTTTTATTGGTGAAAATTGACAAATTATCTAGTCTCGATACACTGTCGTCTTTATCCTAACATTATCAATCTAGACATTACACTAATTTCTATATACTTTTGTTGTTGAATTTCCACTTTGGAAGATTCACATAATGTTTTATATGTGTAACCAAAGCACTCCAAATCCTTTGTCGAAAAGTTCTATCTTGTACTTACTAGAACTCGAATCATATGATAAACAGATACAAATCCTTTTGGCATGGGTTTAAAGCTCCAAAGATTGCCCCAATCGCTTTCTGATGCTTTAGAAGCTTTGGAAAAAAACAATACGTTCACAGATCTTATAGGTGAAAAGTTGTTGGTTGCCATAAAGACAATTCGCAAGGTATGTATTTAATTTAATTAACTTTTCATTAGTTTTGTTAAAGCATTTAATTCTTCCTCATACAACCTCAATATTATTCATTTTATTTCTAACTTTCAAAGATTGAGAGAGGTACAATCTATAAAACAATTTATCATTTGCAGGCAGAAGTGAAATATTACTCCGAGCATCAGGATGCATACAAGCAACTTATACAATACTATTAA

mRNA sequence

ATGGATTCGAGTATTTGTGAGAATGGAGTTGATATCGGAGGCGATGCCAACAATCTGGGAGATGTTGTGCAAAAGGATACCAAGCTTGTTCGGATTATTTGGGTTGATGCTTCAGGACTCCATAGATGTCGTATTATTCCCTCTATGCGGTTCAATGATGTGGTTAAAAAGAGTGGGATTGGTTTATCTGTTTCATGTATGGCAAGGCCTTCTTTTAGCGATATTCCAGCGGGGCGTAATCTTACTGGTGTTGGTGAGATGAGACTGCTACCAGATTTATCAACCAAACGGACGGTTCCTTGGAGGGAGCAAGAGGAAATGGTAATGGGTGACATGTATATTAGACCTGGTGAATCATGGGAATACTGTCCAAGGGAAGCCTTACGTAGGGTCTCTAGAATTCTGAAAAATGAATTTGACTTGGAAATGAAAGCAGGCTTTGAAATCGAGTTTTTGCTATTAAAAAAGGCAGTCAAAGATGGAAAAGAAGATTGGGTGCCATTTGATTCATCATTCTATTGTTCTGCATCTTCATATGATGCTGTCTCTCCCTTTTTTCATGACGTAGTTGCTTCTTTGAACTCCTTGAATATTATTGTGGAACAGTTGCATGCAGAGGTTGGAAATGGTCAATTTGAGTTTGCTTTAGGGTATACTTCTTGTCTCAGTGCTGCTGACGAATTAATTTACACTCGTGAAGTGATTAAGGCTATTGCAAGAAAGCATGGATTGTTGGCAACATTTATACCCAAGTATGCAATGGATGACATGGGTTCTGGTTCCCACGTGCATATCAGTCTGTGGCGGAATGGAAAAAATGTTCTGATGGCAGCTGACAAATCTTCTAAGCATGGGATGTCAACAATTGGTGAAGAGTTCATGGCTGGCGTTTTCAATCATATTACATCAATTTTGCCTTTTATAGCTCCAGTTCCCAACAGTTATGACCGTATACAACCCAATACATGGTCTGGAGCTTATCATTGTTGGGGTAAAGATAACAAAGAAGCTCCAATTAGAACAGCCAGTCCACCTGGAATTGGTGTTAGTTTAGTGAGTAATTTTGAGGTCAAATCATTTGACGGTTGTGCAAACCCATATTTGGGTCTAGCTGCTATCCTTTGTGCTGGATTAGATGGCCTTCGTAACCATCTTCAGTTGCCTGAACCTATCGATACAAATCCTTTTGGCATGGGTTTAAAGCTCCAAAGATTGCCCCAATCGCTTTCTGATGCTTTAGAAGCTTTGGAAAAAAACAATACGTTCACAGATCTTATAGGTGAAAAGTTGTTGGTTGCCATAAAGACAATTCGCAAGGCAGAAGTGAAATATTACTCCGAGCATCAGGATGCATACAAGCAACTTATACAATACTATTAA

Coding sequence (CDS)

ATGGATTCGAGTATTTGTGAGAATGGAGTTGATATCGGAGGCGATGCCAACAATCTGGGAGATGTTGTGCAAAAGGATACCAAGCTTGTTCGGATTATTTGGGTTGATGCTTCAGGACTCCATAGATGTCGTATTATTCCCTCTATGCGGTTCAATGATGTGGTTAAAAAGAGTGGGATTGGTTTATCTGTTTCATGTATGGCAAGGCCTTCTTTTAGCGATATTCCAGCGGGGCGTAATCTTACTGGTGTTGGTGAGATGAGACTGCTACCAGATTTATCAACCAAACGGACGGTTCCTTGGAGGGAGCAAGAGGAAATGGTAATGGGTGACATGTATATTAGACCTGGTGAATCATGGGAATACTGTCCAAGGGAAGCCTTACGTAGGGTCTCTAGAATTCTGAAAAATGAATTTGACTTGGAAATGAAAGCAGGCTTTGAAATCGAGTTTTTGCTATTAAAAAAGGCAGTCAAAGATGGAAAAGAAGATTGGGTGCCATTTGATTCATCATTCTATTGTTCTGCATCTTCATATGATGCTGTCTCTCCCTTTTTTCATGACGTAGTTGCTTCTTTGAACTCCTTGAATATTATTGTGGAACAGTTGCATGCAGAGGTTGGAAATGGTCAATTTGAGTTTGCTTTAGGGTATACTTCTTGTCTCAGTGCTGCTGACGAATTAATTTACACTCGTGAAGTGATTAAGGCTATTGCAAGAAAGCATGGATTGTTGGCAACATTTATACCCAAGTATGCAATGGATGACATGGGTTCTGGTTCCCACGTGCATATCAGTCTGTGGCGGAATGGAAAAAATGTTCTGATGGCAGCTGACAAATCTTCTAAGCATGGGATGTCAACAATTGGTGAAGAGTTCATGGCTGGCGTTTTCAATCATATTACATCAATTTTGCCTTTTATAGCTCCAGTTCCCAACAGTTATGACCGTATACAACCCAATACATGGTCTGGAGCTTATCATTGTTGGGGTAAAGATAACAAAGAAGCTCCAATTAGAACAGCCAGTCCACCTGGAATTGGTGTTAGTTTAGTGAGTAATTTTGAGGTCAAATCATTTGACGGTTGTGCAAACCCATATTTGGGTCTAGCTGCTATCCTTTGTGCTGGATTAGATGGCCTTCGTAACCATCTTCAGTTGCCTGAACCTATCGATACAAATCCTTTTGGCATGGGTTTAAAGCTCCAAAGATTGCCCCAATCGCTTTCTGATGCTTTAGAAGCTTTGGAAAAAAACAATACGTTCACAGATCTTATAGGTGAAAAGTTGTTGGTTGCCATAAAGACAATTCGCAAGGCAGAAGTGAAATATTACTCCGAGCATCAGGATGCATACAAGCAACTTATACAATACTATTAA

Protein sequence

MDSSICENGVDIGGDANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIKTIRKAEVKYYSEHQDAYKQLIQYY
Homology
BLAST of HG10017032 vs. NCBI nr
Match: TYK07337.1 (type-1 glutamine synthetase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 801.6 bits (2069), Expect = 3.6e-228
Identity = 387/459 (84.31%), Postives = 415/459 (90.41%), Query Frame = 0

Query: 1   MDSSICENGVDIGGDANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGI 60
           MD S CEN     G  + +  VV K+ KLVRIIWVDASG HRCR+IPS RFNDVVKK+GI
Sbjct: 1   MDLSNCEN-----GGVHEVKVVVSKEVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGI 60

Query: 61  GLSVSCMARPSFSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESW 120
           GLSV CMAR S +D+P G+NLTG GE+RLLPDLSTKRT+PW EQEEMVMGDMYI+PGE+W
Sbjct: 61  GLSVVCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTIPWMEQEEMVMGDMYIKPGEAW 120

Query: 121 EYCPREALRRVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYD 180
           EYCPREALRRVS ILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYD
Sbjct: 121 EYCPREALRRVSTILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYD 180

Query: 181 AVSPFFHDVVASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIAR 240
           A +PF HDVV SLNSLNI VEQLHAE G GQFEFALGYT+CL+AAD+L+YTREVIKA+AR
Sbjct: 181 AAAPFLHDVVDSLNSLNITVEQLHAEAGKGQFEFALGYTTCLTAADDLVYTREVIKAVAR 240

Query: 241 KHGLLATFIPKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNH 300
           KHGLLATFIPKYAMDD+GSG HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  H
Sbjct: 241 KHGLLATFIPKYAMDDVGSGCHVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYH 300

Query: 301 ITSILPFIAPVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSF 360
           I SILPFIAP+PNSYDRIQPNTW GAYHCWGKDN+EAPIRTASPPGIGV+ VSNFEVKSF
Sbjct: 301 IPSILPFIAPLPNSYDRIQPNTWCGAYHCWGKDNREAPIRTASPPGIGVTSVSNFEVKSF 360

Query: 361 DGCANPYLGLAAILCAGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNN 420
           DGCANPYLGLAAILCAGLDGLR HLQLP+PIDTNP  MGLK QRLPQSLS+ALE LEKNN
Sbjct: 361 DGCANPYLGLAAILCAGLDGLRYHLQLPQPIDTNPSYMGLKFQRLPQSLSEALEHLEKNN 420

Query: 421 TFTDLIGEKLLVAIKTIRKAEVKYYSEHQDAYKQLIQYY 460
           TFTDLIGE+LL AIK IRKAEVKYYS+HQDAYKQLIQ+Y
Sbjct: 421 TFTDLIGERLLAAIKEIRKAEVKYYSKHQDAYKQLIQHY 454

BLAST of HG10017032 vs. NCBI nr
Match: XP_008462507.1 (PREDICTED: type-1 glutamine synthetase 1-like [Cucumis melo])

HSP 1 Score: 793.5 bits (2048), Expect = 9.7e-226
Identity = 384/459 (83.66%), Postives = 413/459 (89.98%), Query Frame = 0

Query: 1   MDSSICENGVDIGGDANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGI 60
           MD S CEN     G  + +  VV K+ KLVRIIWVDASG HRCR+IPS RFNDVVKK+GI
Sbjct: 1   MDLSNCEN-----GGVHEVKVVVSKEVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGI 60

Query: 61  GLSVSCMARPSFSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESW 120
           GLSV CMAR S +D+P G+NLTG GE+RLLPDLSTKRTVPW EQEEMVMGDMYI+PGE+W
Sbjct: 61  GLSVVCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTVPWMEQEEMVMGDMYIKPGEAW 120

Query: 121 EYCPREALRRVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYD 180
           EYCPREALRRV  +LKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYD
Sbjct: 121 EYCPREALRRVFTVLKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYD 180

Query: 181 AVSPFFHDVVASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIAR 240
           A +PF HDVV SLNSLNI VEQLHAE G GQFEFALGYT+CL+AAD+L+YTREVIKA+AR
Sbjct: 181 AAAPFLHDVVDSLNSLNITVEQLHAEAGKGQFEFALGYTTCLTAADDLVYTREVIKAVAR 240

Query: 241 KHGLLATFIPKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNH 300
           KHGLLATFIPKYAMDD+GSG HVHISLW+NGKNVLMA DKSSKHGMSTIGEEFMAGV  H
Sbjct: 241 KHGLLATFIPKYAMDDVGSGCHVHISLWQNGKNVLMATDKSSKHGMSTIGEEFMAGVLYH 300

Query: 301 ITSILPFIAPVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSF 360
           I SILPFIAP+PNSYDRIQPNT+SGAYHCWGKDN+E PIRTAS PGIGV+LVSNFEVKSF
Sbjct: 301 IPSILPFIAPLPNSYDRIQPNTFSGAYHCWGKDNREVPIRTASSPGIGVTLVSNFEVKSF 360

Query: 361 DGCANPYLGLAAILCAGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNN 420
           DGCANPYLGLAAILCAGLDGLR HLQLP+PIDTNP  MGLK QRLPQSLS+ALE LEKNN
Sbjct: 361 DGCANPYLGLAAILCAGLDGLRYHLQLPQPIDTNPSYMGLKFQRLPQSLSEALEHLEKNN 420

Query: 421 TFTDLIGEKLLVAIKTIRKAEVKYYSEHQDAYKQLIQYY 460
           TFTDLIGE+LL AIK IRKAEVKYYS+HQDAYKQLIQ+Y
Sbjct: 421 TFTDLIGERLLAAIKEIRKAEVKYYSKHQDAYKQLIQHY 454

BLAST of HG10017032 vs. NCBI nr
Match: XP_011657676.1 (type-1 glutamine synthetase 1 [Cucumis sativus])

HSP 1 Score: 786.6 bits (2030), Expect = 1.2e-223
Identity = 375/438 (85.62%), Postives = 404/438 (92.24%), Query Frame = 0

Query: 22  VVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDIPAGRNL 81
           VV  + KLVRIIWVDASG HRCR+IPS RFNDVVKK+G+GLSV CMAR S++D+P G+NL
Sbjct: 17  VVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPIGKNL 76

Query: 82  TGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRILKNEFDL 141
           TG GE+RLLPDLSTKR VPW EQEEMVMGDMYIRPGE+WEYCPREALRR+S ILKNEFDL
Sbjct: 77  TGAGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPREALRRLSSILKNEFDL 136

Query: 142 EMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNSLNIIVE 201
           EMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYDA +PFFHDVV SLNSLNI VE
Sbjct: 137 EMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYDATAPFFHDVVDSLNSLNITVE 196

Query: 202 QLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGSGS 261
           QLHAE   GQFEFALGYT+CL+AADELIYTREVIKA+ARKHGLLATFIPKYAMDD+GSG 
Sbjct: 197 QLHAEAAKGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMDDVGSGC 256

Query: 262 HVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQPN 321
           HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  HI SILPFIAP+PNSYDRIQPN
Sbjct: 257 HVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSYDRIQPN 316

Query: 322 TWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLDGL 381
           TW GAYHCWGKDN+EAPIRTASPPGIGV+ VSNFEVKSFDG ANPYLGLA+ILCAGLDGL
Sbjct: 317 TWCGAYHCWGKDNREAPIRTASPPGIGVTSVSNFEVKSFDGLANPYLGLASILCAGLDGL 376

Query: 382 RNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIKTIRKAE 441
           RNH+QLP+PIDTNP  MGLK QRLPQSLS+ALEALE NNTFTDLIGE+LL AIK IRKAE
Sbjct: 377 RNHIQLPQPIDTNPSYMGLKFQRLPQSLSEALEALENNNTFTDLIGERLLAAIKEIRKAE 436

Query: 442 VKYYSEHQDAYKQLIQYY 460
           V+YY++HQDAYKQLI +Y
Sbjct: 437 VEYYTKHQDAYKQLIHHY 454

BLAST of HG10017032 vs. NCBI nr
Match: KGN48473.2 (hypothetical protein Csa_004298 [Cucumis sativus])

HSP 1 Score: 771.2 bits (1990), Expect = 5.2e-219
Identity = 370/444 (83.33%), Postives = 402/444 (90.54%), Query Frame = 0

Query: 16  ANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDI 75
           +NN  +     TK+VRIIWVD SG HRCR+IPS RFNDVVKK+G+GLSV CMAR S ++I
Sbjct: 4   SNNCENGGVNQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSHTEI 63

Query: 76  PAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRIL 135
           P G++LTGVGE+RLLPDLSTKR VPW EQEEMVMGDMYIRPGE+WEYCPRE LRR+S IL
Sbjct: 64  PIGKDLTGVGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPRETLRRLSSIL 123

Query: 136 KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNS 195
           KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS  SYDA +PFFHD+V  LNS
Sbjct: 124 KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSSYCSTFSYDAAAPFFHDIVDCLNS 183

Query: 196 LNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMD 255
           LNI VEQLHAE   GQFEFALGYT+CL+AADELIYTREVIKA+ARKHGLLATFIPKYAMD
Sbjct: 184 LNITVEQLHAEAAEGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMD 243

Query: 256 DMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSY 315
           D+GSG HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  HI SILPFIAP+PNSY
Sbjct: 244 DVGSGCHVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSY 303

Query: 316 DRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILC 375
           DRIQPNT  GAYHCWGKDN+EAPIRTASPPGIGV+LVSNFEVKSFDG ANPYLGLA+ILC
Sbjct: 304 DRIQPNTLCGAYHCWGKDNREAPIRTASPPGIGVNLVSNFEVKSFDGLANPYLGLASILC 363

Query: 376 AGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIK 435
           AGLDGLRNH+QLP+PIDTNP  MGLK QRLPQSLS+ALEALE NNTFTDLIGE+LL AIK
Sbjct: 364 AGLDGLRNHIQLPQPIDTNPSYMGLKFQRLPQSLSEALEALENNNTFTDLIGERLLAAIK 423

Query: 436 TIRKAEVKYYSEHQDAYKQLIQYY 460
            IRKAEV+YY++HQDAYKQLI +Y
Sbjct: 424 EIRKAEVEYYTKHQDAYKQLIHHY 447

BLAST of HG10017032 vs. NCBI nr
Match: XP_011657677.1 (type-1 glutamine synthetase 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 760.0 bits (1961), Expect = 1.2e-215
Identity = 366/444 (82.43%), Postives = 398/444 (89.64%), Query Frame = 0

Query: 16  ANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDI 75
           +NN  +     TK+VRIIWVD SG HRCR+IPS RFNDVVKK+G+GLSV CMAR S ++I
Sbjct: 4   SNNCENGGVNQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSHTEI 63

Query: 76  PAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRIL 135
           P G++LTGVGE+RLLPDLSTKR VPW EQEEMVMGDMYIRPGE+WEYCPRE LRR+S IL
Sbjct: 64  PIGKDLTGVGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPRETLRRLSSIL 123

Query: 136 KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNS 195
           KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS  SYDA +PFFHD+V  LNS
Sbjct: 124 KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSSYCSTFSYDAAAPFFHDIVDCLNS 183

Query: 196 LNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMD 255
           LNI VEQLHAE   GQFEFALGYT+CL+AADELIYTREVIKA+ARKHGLLATFIPKYAMD
Sbjct: 184 LNITVEQLHAEAAEGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMD 243

Query: 256 DMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSY 315
           D+GSG HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  HI SILPFIAP+PNSY
Sbjct: 244 DVGSGCHVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSY 303

Query: 316 DRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILC 375
           DRIQPNT  GAYHCWGKDN+EAPIRTASPPGIGV+LVSNFEVKSFDG ANPYLGLA+ILC
Sbjct: 304 DRIQPNTLCGAYHCWGKDNREAPIRTASPPGIGVNLVSNFEVKSFDGLANPYLGLASILC 363

Query: 376 AGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIK 435
           AGLDGLRNH+QLP+PIDTNP  M LK QRLPQSLS+ALEALE NN FT+LIGE+LL AIK
Sbjct: 364 AGLDGLRNHIQLPQPIDTNPSYMDLKFQRLPQSLSEALEALENNNMFTNLIGERLLTAIK 423

Query: 436 TIRKAEVKYYSEHQDAYKQLIQYY 460
            IRKAEV +YS+HQ+AYKQLI  Y
Sbjct: 424 EIRKAEVNHYSKHQEAYKQLIHRY 447

BLAST of HG10017032 vs. ExPASy Swiss-Prot
Match: Q86B00 (Type-1 glutamine synthetase 1 OS=Dictyostelium discoideum OX=44689 GN=glnA1 PE=3 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 5.6e-59
Identity = 144/440 (32.73%), Postives = 214/440 (48.64%), Query Frame = 0

Query: 28  KLVRIIWVDASGLHRCRIIP-SMRFNDVVKKSGIGLSVSCMARPSFSDIPAGRNL--TGV 87
           K +R+ W+D S   R + I      N   K   + ++  CM+   F D      L     
Sbjct: 53  KFIRVCWIDISNKIRTKAINIDWILNHEPKLIHVSITNVCMSLLCFEDSVTIEALKSENF 112

Query: 88  GEMRLLPDLSTKRTV-PWREQEEMVMGDMYIRPGES-----WEYCPREALRRVSRILKNE 147
           GE  L+P  +TK  + P+      + G+ +    ES     W  CPR +L+R    LKN+
Sbjct: 113 GEAFLIPITTTKLNILPYCPSHIQIFGEFFYLDNESKKLKPWLLCPRNSLQRAIDRLKNK 172

Query: 148 FDLEMKAGFEIEFLLLKK------AVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVAS 207
           F + +K  FE EF L+KK      +V          D   + +  S D        +  +
Sbjct: 173 FGISLKGSFEEEFYLIKKGDNNNSSVASLLNSIEKLDHGTFANYHSLDCYGDILEKITNA 232

Query: 208 LNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKY 267
           L    + +EQL +E G+GQFE  + YT  + A D  I  R+ I +IA  +G +ATFIPK 
Sbjct: 233 LEEQGLPIEQLLSESGSGQFEITIDYTDIMEACDRHIIVRQTINSIASYNGYIATFIPKP 292

Query: 268 AMDDMGSGSHVHISLW-RNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPV 327
               +GSG H H+SLW  N  N  +  D + + G+S + + F+ G+  H  S+       
Sbjct: 293 FDGLVGSGCHAHLSLWDTNDSN--LTPDANGECGLSLVNQFFIGGLLKHSKSLTALFNTT 352

Query: 328 PNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLA 387
           PNSY R++P  WSG    WG DNKE+ IR  S P       SNFE+K+ D  +NPYL +A
Sbjct: 353 PNSYKRLKPFYWSGCNVSWGLDNKESFIRIPSSPFSATDGCSNFEIKTIDHTSNPYLAMA 412

Query: 388 AILCAGLDGLRNHLQLPEPIDTNPFGMG-LKLQRLPQSLSDALEALEKNNTFTDLIGEKL 447
            I+ AG DG+ N +  P P  T+ F    L  Q +P +  DA+++L++N+   + IG  +
Sbjct: 413 GIIHAGFDGIENSIAPPPP--TSLFDQSVLNNQLIPSNFEDAIQSLKENHYLCENIGNDI 472

Query: 448 LVAIKTIRKAEVKYYSEHQD 451
             A   ++ AE K   E  D
Sbjct: 473 SNAFIHVKLAENKILKELSD 488

BLAST of HG10017032 vs. ExPASy Swiss-Prot
Match: P38094 (Protein fluG OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=fluG PE=1 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.7e-52
Identity = 146/451 (32.37%), Postives = 223/451 (49.45%), Query Frame = 0

Query: 30  VRIIW---VDASGLHRCRIIPSMRFNDVVKKS-GIGLSVSCMARPSFSDIPAGRNLTGVG 89
           V+ +W   +D +   R R+ P M F  +V+K   +G+S++        ++  G   +  G
Sbjct: 444 VKYVWTQFIDYTATVRVRMFPVMEFAKIVRKQRRLGISMATFWMLQDDEVVGG---STTG 503

Query: 90  EMRLLPDLST-KRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRILKNEFDLEMK 149
           +  L+PDLST    V    +   VM       GES E CPR  L  ++  LK+EF ++  
Sbjct: 504 QFYLIPDLSTLSPNVGIDSKSATVMTWWKSEQGESLEECPRTNLLNINNKLKDEFGIQAT 563

Query: 150 AGFEIEFLLLKKAV--KDGKEDWVPFDSSFYCSASSYDA--VSPFFHDVVASLNSLNIIV 209
            GFEIE + LK       G+EDW P  ++   S  + +   + P   ++  +L S+ I +
Sbjct: 564 CGFEIEVVFLKPTTDPSTGEEDWAPSVTNHSWSQMTRETRRMLPLLEEIAETLASIGIHL 623

Query: 210 EQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGSG 269
           +Q HAE   GQFEF L   + ++A D LI +R+VI  I  KHGL AT  P+      G+ 
Sbjct: 624 QQFHAESAPGQFEFILPPDNPVAAVDTLIKSRQVIANIVEKHGLRATLYPRPYPSAAGTA 683

Query: 270 SHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQP 329
           SH H+S+                   ST  E F+AGV  H  ++L F      SYDR++ 
Sbjct: 684 SHAHVSI-----------------SPSTKEESFLAGVLQHYPAVLAFTLSGDASYDRVKS 743

Query: 330 NTWSGA-YHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLD 389
             W+G+ +  WG  N+EAPIR  SP         ++E+KS DG AN YL +AA L AG  
Sbjct: 744 GIWAGSEWVTWGTQNREAPIRKISP--------GHWEIKSLDGLANMYLAMAAFLAAGYT 803

Query: 390 GLRNHLQLPE---PID------TNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKL 449
           G++ +L L     P D      +    +G+   +LP +L+ +L ALE +     L+GE L
Sbjct: 804 GVKENLPLTIKDCPYDAASLPESERAALGI-TTKLPNTLAKSLAALESDEILRSLLGENL 863

Query: 450 LVAIKTIRKAEVKYYS--EHQDAYKQLIQYY 460
           +     +++AE K  S  + +   K L++ Y
Sbjct: 864 VEDYIIVKRAESKKLSAMDEKARRKWLVERY 865

BLAST of HG10017032 vs. ExPASy Swiss-Prot
Match: O59648 (Glutamine synthetase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=glnA PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 9.9e-48
Identity = 118/365 (32.33%), Postives = 175/365 (47.95%), Query Frame = 0

Query: 86  EMRLLPDLSTKRTVPWREQEE---MVMGDMYIRPGESWEYCPREALRRVSRILKNEFDLE 145
           +M L PDL T   +PWR  E+    V+ D+Y   G+ +E  PR  L+RV      E   E
Sbjct: 72  DMMLKPDLKTLSVLPWRPTEKSVARVICDVYTTNGKPFEGDPRGCLKRVLAKFDEELGGE 131

Query: 146 MKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNSLNIIVEQ 205
              G E EF +LK   +D    WVP D + Y      D        +V +L +L   VE 
Sbjct: 132 FFVGPEPEFFILK---EDACGSWVPADDAGYFDLEPLDGGCDIRRKIVFALENLGFHVEA 191

Query: 206 LHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGSGSH 265
            H EV  GQ E    +   +  AD ++  +  IK +A + GL ATF+PK      GSG H
Sbjct: 192 SHHEVAEGQHEVDFKFADAVKTADSVVTFKTTIKTLAAQDGLKATFMPKPFFGINGSGMH 251

Query: 266 VHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQPNT 325
            H S+W NG+      D+S+++ +S     ++AG+ +H  SI+    P  NSY R+ P  
Sbjct: 252 CHQSIWLNGEPSFY--DESAQYQLSETCMSYVAGILDHAKSIVAVTNPTVNSYKRLVPGY 311

Query: 326 WSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLDGLR 385
            +     W   N+ A +R  +P G G    +  E ++ D   NPYL    +L AGLDG++
Sbjct: 312 EAPVNIAWANSNRSAIVRVPAPRGKG----TRIEFRAPDPACNPYLAFTVMLAAGLDGVK 371

Query: 386 NHLQLPEPIDTNPFGMGLK------LQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIKT 442
             L   EP++ N F M         ++ +P +L  AL+ LE N+   D +G+ +      
Sbjct: 372 RKLSAIEPVEKNIFAMSEAQKKAEGIESVPANLKAALDELENNSVLKDALGKHIFENFIE 427

BLAST of HG10017032 vs. ExPASy Swiss-Prot
Match: P21154 (Glutamine synthetase OS=Methanococcus voltae OX=2188 GN=glnA PE=3 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.7e-47
Identity = 120/369 (32.52%), Postives = 182/369 (49.32%), Query Frame = 0

Query: 86  EMRLLPDLSTKRTVPWREQEEMV---MGDMYIRPGESWEYCPREALRRVSRILKNEFDLE 145
           +M L PDLST   +PWR  E+ V   + D+Y + G+ +E  PR  L+RV    K EF  E
Sbjct: 73  DMVLKPDLSTLSVLPWRPSEKSVARIICDVYRKNGKPFEGDPRGCLKRVLAEFKEEFKGE 132

Query: 146 MKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNSLNIIVEQ 205
              G E EF +LK   ++GK  WVP D + Y      D  +    ++V +L +L   VE 
Sbjct: 133 YFVGPEPEFFILKN--ENGK--WVPGDDAGYFELEPLDEGNDLRRNIVFALENLGFHVEA 192

Query: 206 LHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGSGSH 265
            H EV  GQ E    Y + +  AD +I  +  IK +A++ G+LATF+PK      GSG H
Sbjct: 193 SHHEVAPGQHEVDFKYDNAVKTADSVITFKTTIKTLAKQSGVLATFMPKPFFGMNGSGMH 252

Query: 266 VHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQPNT 325
            + S+W +GK      D+++ H +S I   ++ G+  H  +++    P  NSY R+ P  
Sbjct: 253 CNQSIWLDGKPSFY--DENNAHQLSDICLSYIGGILEHTKALVSVTNPTVNSYKRLVPGY 312

Query: 326 WSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLDGLR 385
            +     W   N+ + IR  +  G G    +  E ++ D   NPYL    +L AGLDG+R
Sbjct: 313 EAPVNIAWANSNRTSIIRVPAARGKG----TRVEFRAPDPACNPYLAFTVMLAAGLDGVR 372

Query: 386 NHLQLPEPIDTNPFGMG------LKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIKT 445
             L  PEP++ N F M         +  +P +L DA+E L ++      +G+ +      
Sbjct: 373 RKLIAPEPVEKNIFAMSEAEKREANIDSVPSNLYDAIEELRQDKVLKKALGDHIFNKFIE 431

BLAST of HG10017032 vs. ExPASy Swiss-Prot
Match: Q60182 (Glutamine synthetase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=glnA PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.9e-47
Identity = 122/378 (32.28%), Postives = 182/378 (48.15%), Query Frame = 0

Query: 78  GRNLTGV-----GEMRLLPDLSTKRTVPWREQEE---MVMGDMYIRPGESWEYCPREALR 137
           G ++TG       +M L PDLST   +PWR +E+    V+ D+Y      +E  PR  L+
Sbjct: 64  GSSITGFVGIEESDMLLKPDLSTLSVLPWRPEEKSVARVICDVYKDEKTPFEGDPRSRLK 123

Query: 138 RVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDV 197
            +   LK E + E   G E EF LLK+        WVP D   Y      D       D+
Sbjct: 124 AILEELKKEMNGEYFVGPEPEFFLLKRD-PHNPHRWVPADDGGYFDVEPLDDAPDIRRDI 183

Query: 198 VASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFI 257
           V +L +L   VE  H EV  GQ E    + + L  AD +I  +  IK IA+KHGL ATF+
Sbjct: 184 VLALENLGFHVEASHHEVAPGQHEVDFKFDNALKTADSVITFKMTIKNIAKKHGLKATFM 243

Query: 258 PKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIA 317
           PK      G+G H H S+W NG+      +    +G+S     ++AG+ +H  +++    
Sbjct: 244 PKPFFGMNGNGMHCHQSVWFNGEPSFYDPE-GPYNGLSETCLSYIAGILSHAKALVAITN 303

Query: 318 PVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLG 377
           P  NSY R+ P   +     W   N+ A IR  +  G      +  E ++ D   NPYL 
Sbjct: 304 PTVNSYKRLVPGYEAPVNIAWANKNRSAIIRVPAARG----KATRIEFRAPDPTCNPYLA 363

Query: 378 LAAILCAGLDGLRNHLQLPEPIDTNPFGMG------LKLQRLPQSLSDALEALEKNNTFT 437
            A +L AGLDG++  +  PEP++ N F M       L ++ +P +L+ AL+ LE +    
Sbjct: 364 FACMLAAGLDGIKKKMTAPEPVERNIFKMSEEEKKQLGIESVPANLAAALDELECDEVLQ 423

Query: 438 DLIGEKLLVAIKTIRKAE 442
             +G+ +      I++AE
Sbjct: 424 KALGKHIYENYMEIKRAE 435

BLAST of HG10017032 vs. ExPASy TrEMBL
Match: A0A5D3C627 (Type-1 glutamine synthetase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00530 PE=3 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.7e-228
Identity = 387/459 (84.31%), Postives = 415/459 (90.41%), Query Frame = 0

Query: 1   MDSSICENGVDIGGDANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGI 60
           MD S CEN     G  + +  VV K+ KLVRIIWVDASG HRCR+IPS RFNDVVKK+GI
Sbjct: 1   MDLSNCEN-----GGVHEVKVVVSKEVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGI 60

Query: 61  GLSVSCMARPSFSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESW 120
           GLSV CMAR S +D+P G+NLTG GE+RLLPDLSTKRT+PW EQEEMVMGDMYI+PGE+W
Sbjct: 61  GLSVVCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTIPWMEQEEMVMGDMYIKPGEAW 120

Query: 121 EYCPREALRRVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYD 180
           EYCPREALRRVS ILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYD
Sbjct: 121 EYCPREALRRVSTILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYD 180

Query: 181 AVSPFFHDVVASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIAR 240
           A +PF HDVV SLNSLNI VEQLHAE G GQFEFALGYT+CL+AAD+L+YTREVIKA+AR
Sbjct: 181 AAAPFLHDVVDSLNSLNITVEQLHAEAGKGQFEFALGYTTCLTAADDLVYTREVIKAVAR 240

Query: 241 KHGLLATFIPKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNH 300
           KHGLLATFIPKYAMDD+GSG HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  H
Sbjct: 241 KHGLLATFIPKYAMDDVGSGCHVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYH 300

Query: 301 ITSILPFIAPVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSF 360
           I SILPFIAP+PNSYDRIQPNTW GAYHCWGKDN+EAPIRTASPPGIGV+ VSNFEVKSF
Sbjct: 301 IPSILPFIAPLPNSYDRIQPNTWCGAYHCWGKDNREAPIRTASPPGIGVTSVSNFEVKSF 360

Query: 361 DGCANPYLGLAAILCAGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNN 420
           DGCANPYLGLAAILCAGLDGLR HLQLP+PIDTNP  MGLK QRLPQSLS+ALE LEKNN
Sbjct: 361 DGCANPYLGLAAILCAGLDGLRYHLQLPQPIDTNPSYMGLKFQRLPQSLSEALEHLEKNN 420

Query: 421 TFTDLIGEKLLVAIKTIRKAEVKYYSEHQDAYKQLIQYY 460
           TFTDLIGE+LL AIK IRKAEVKYYS+HQDAYKQLIQ+Y
Sbjct: 421 TFTDLIGERLLAAIKEIRKAEVKYYSKHQDAYKQLIQHY 454

BLAST of HG10017032 vs. ExPASy TrEMBL
Match: A0A1S3CIM4 (type-1 glutamine synthetase 1-like OS=Cucumis melo OX=3656 GN=LOC103500842 PE=3 SV=1)

HSP 1 Score: 793.5 bits (2048), Expect = 4.7e-226
Identity = 384/459 (83.66%), Postives = 413/459 (89.98%), Query Frame = 0

Query: 1   MDSSICENGVDIGGDANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGI 60
           MD S CEN     G  + +  VV K+ KLVRIIWVDASG HRCR+IPS RFNDVVKK+GI
Sbjct: 1   MDLSNCEN-----GGVHEVKVVVSKEVKLVRIIWVDASGQHRCRVIPSTRFNDVVKKNGI 60

Query: 61  GLSVSCMARPSFSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESW 120
           GLSV CMAR S +D+P G+NLTG GE+RLLPDLSTKRTVPW EQEEMVMGDMYI+PGE+W
Sbjct: 61  GLSVVCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTVPWMEQEEMVMGDMYIKPGEAW 120

Query: 121 EYCPREALRRVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYD 180
           EYCPREALRRV  +LKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYD
Sbjct: 121 EYCPREALRRVFTVLKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYD 180

Query: 181 AVSPFFHDVVASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIAR 240
           A +PF HDVV SLNSLNI VEQLHAE G GQFEFALGYT+CL+AAD+L+YTREVIKA+AR
Sbjct: 181 AAAPFLHDVVDSLNSLNITVEQLHAEAGKGQFEFALGYTTCLTAADDLVYTREVIKAVAR 240

Query: 241 KHGLLATFIPKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNH 300
           KHGLLATFIPKYAMDD+GSG HVHISLW+NGKNVLMA DKSSKHGMSTIGEEFMAGV  H
Sbjct: 241 KHGLLATFIPKYAMDDVGSGCHVHISLWQNGKNVLMATDKSSKHGMSTIGEEFMAGVLYH 300

Query: 301 ITSILPFIAPVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSF 360
           I SILPFIAP+PNSYDRIQPNT+SGAYHCWGKDN+E PIRTAS PGIGV+LVSNFEVKSF
Sbjct: 301 IPSILPFIAPLPNSYDRIQPNTFSGAYHCWGKDNREVPIRTASSPGIGVTLVSNFEVKSF 360

Query: 361 DGCANPYLGLAAILCAGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNN 420
           DGCANPYLGLAAILCAGLDGLR HLQLP+PIDTNP  MGLK QRLPQSLS+ALE LEKNN
Sbjct: 361 DGCANPYLGLAAILCAGLDGLRYHLQLPQPIDTNPSYMGLKFQRLPQSLSEALEHLEKNN 420

Query: 421 TFTDLIGEKLLVAIKTIRKAEVKYYSEHQDAYKQLIQYY 460
           TFTDLIGE+LL AIK IRKAEVKYYS+HQDAYKQLIQ+Y
Sbjct: 421 TFTDLIGERLLAAIKEIRKAEVKYYSKHQDAYKQLIQHY 454

BLAST of HG10017032 vs. ExPASy TrEMBL
Match: A0A0A0KES4 (Gln-synt_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G448140 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 5.7e-224
Identity = 375/438 (85.62%), Postives = 404/438 (92.24%), Query Frame = 0

Query: 22  VVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDIPAGRNL 81
           VV  + KLVRIIWVDASG HRCR+IPS RFNDVVKK+G+GLSV CMAR S++D+P G+NL
Sbjct: 17  VVPNEVKLVRIIWVDASGHHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSYTDVPIGKNL 76

Query: 82  TGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRILKNEFDL 141
           TG GE+RLLPDLSTKR VPW EQEEMVMGDMYIRPGE+WEYCPREALRR+S ILKNEFDL
Sbjct: 77  TGAGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPREALRRLSSILKNEFDL 136

Query: 142 EMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNSLNIIVE 201
           EMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYDA +PFFHDVV SLNSLNI VE
Sbjct: 137 EMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYDATAPFFHDVVDSLNSLNITVE 196

Query: 202 QLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGSGS 261
           QLHAE   GQFEFALGYT+CL+AADELIYTREVIKA+ARKHGLLATFIPKYAMDD+GSG 
Sbjct: 197 QLHAEAAKGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMDDVGSGC 256

Query: 262 HVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQPN 321
           HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  HI SILPFIAP+PNSYDRIQPN
Sbjct: 257 HVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSYDRIQPN 316

Query: 322 TWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLDGL 381
           TW GAYHCWGKDN+EAPIRTASPPGIGV+ VSNFEVKSFDG ANPYLGLA+ILCAGLDGL
Sbjct: 317 TWCGAYHCWGKDNREAPIRTASPPGIGVTSVSNFEVKSFDGLANPYLGLASILCAGLDGL 376

Query: 382 RNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIKTIRKAE 441
           RNH+QLP+PIDTNP  MGLK QRLPQSLS+ALEALE NNTFTDLIGE+LL AIK IRKAE
Sbjct: 377 RNHIQLPQPIDTNPSYMGLKFQRLPQSLSEALEALENNNTFTDLIGERLLAAIKEIRKAE 436

Query: 442 VKYYSEHQDAYKQLIQYY 460
           V+YY++HQDAYKQLI +Y
Sbjct: 437 VEYYTKHQDAYKQLIHHY 454

BLAST of HG10017032 vs. ExPASy TrEMBL
Match: A0A0A0KKB4 (Gln-synt_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G448150 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 5.8e-216
Identity = 366/444 (82.43%), Postives = 398/444 (89.64%), Query Frame = 0

Query: 16  ANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDI 75
           +NN  +     TK+VRIIWVD SG HRCR+IPS RFNDVVKK+G+GLSV CMAR S ++I
Sbjct: 4   SNNCENGGVNQTKVVRIIWVDTSGQHRCRVIPSTRFNDVVKKNGVGLSVVCMARSSHTEI 63

Query: 76  PAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRIL 135
           P G++LTGVGE+RLLPDLSTKR VPW EQEEMVMGDMYIRPGE+WEYCPRE LRR+S IL
Sbjct: 64  PIGKDLTGVGEIRLLPDLSTKRIVPWMEQEEMVMGDMYIRPGEAWEYCPRETLRRLSSIL 123

Query: 136 KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNS 195
           KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS  SYDA +PFFHD+V  LNS
Sbjct: 124 KNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSSYCSTFSYDAAAPFFHDIVDCLNS 183

Query: 196 LNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMD 255
           LNI VEQLHAE   GQFEFALGYT+CL+AADELIYTREVIKA+ARKHGLLATFIPKYAMD
Sbjct: 184 LNITVEQLHAEAAEGQFEFALGYTTCLTAADELIYTREVIKAVARKHGLLATFIPKYAMD 243

Query: 256 DMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSY 315
           D+GSG HVHISLW+NGKNVLMAADKSSKHGMSTIGEEFMAGV  HI SILPFIAP+PNSY
Sbjct: 244 DVGSGCHVHISLWQNGKNVLMAADKSSKHGMSTIGEEFMAGVLYHIPSILPFIAPLPNSY 303

Query: 316 DRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILC 375
           DRIQPNT  GAYHCWGKDN+EAPIRTASPPGIGV+LVSNFEVKSFDG ANPYLGLA+ILC
Sbjct: 304 DRIQPNTLCGAYHCWGKDNREAPIRTASPPGIGVNLVSNFEVKSFDGLANPYLGLASILC 363

Query: 376 AGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIK 435
           AGLDGLRNH+QLP+PIDTNP  M LK QRLPQSLS+ALEALE NN FT+LIGE+LL AIK
Sbjct: 364 AGLDGLRNHIQLPQPIDTNPSYMDLKFQRLPQSLSEALEALENNNMFTNLIGERLLTAIK 423

Query: 436 TIRKAEVKYYSEHQDAYKQLIQYY 460
            IRKAEV +YS+HQ+AYKQLI  Y
Sbjct: 424 EIRKAEVNHYSKHQEAYKQLIHRY 447

BLAST of HG10017032 vs. ExPASy TrEMBL
Match: A0A5D3CAL1 (Type-1 glutamine synthetase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00660 PE=3 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 6.0e-213
Identity = 369/459 (80.39%), Postives = 398/459 (86.71%), Query Frame = 0

Query: 1   MDSSICENGVDIGGDANNLGDVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGI 60
           MD S CEN     G  + +  VV K+ KLV               IPS RFNDVVKK+G+
Sbjct: 1   MDLSNCEN-----GGVHEVKVVVPKEVKLV---------------IPSTRFNDVVKKNGV 60

Query: 61  GLSVSCMARPSFSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESW 120
           GLSV CMAR S +D+P G+NLTG GE+RLLPDLSTKRTVPW EQEEMVMGDMYI+PGE+W
Sbjct: 61  GLSVVCMARSSHTDVPIGKNLTGAGEIRLLPDLSTKRTVPWMEQEEMVMGDMYIKPGEAW 120

Query: 121 EYCPREALRRVSRILKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYD 180
           EYCPREALRRVS +LKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSS YCS SSYD
Sbjct: 121 EYCPREALRRVSTVLKNEFDLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSLYCSTSSYD 180

Query: 181 AVSPFFHDVVASLNSLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIAR 240
           A +P  HDVV SLNSLNI VEQLHAE+  GQFEFALGYT+CL+AAD+L+YTREVIKA+AR
Sbjct: 181 AAAPLLHDVVDSLNSLNITVEQLHAEIAKGQFEFALGYTTCLTAADDLVYTREVIKAVAR 240

Query: 241 KHGLLATFIPKYAMDDMGSGSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNH 300
           KHGLLATFIPKYAMDD+GSG HVHISLW+NGKNVLMA DKSSKHGMSTIGEEFMAGV  H
Sbjct: 241 KHGLLATFIPKYAMDDVGSGCHVHISLWQNGKNVLMATDKSSKHGMSTIGEEFMAGVLYH 300

Query: 301 ITSILPFIAPVPNSYDRIQPNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSF 360
           I SILPFIAP+PNSYDRIQPNT+SGAYHCWGKDN+E PIRTAS PGIGV+LVSNFEVKSF
Sbjct: 301 IPSILPFIAPLPNSYDRIQPNTFSGAYHCWGKDNREVPIRTASSPGIGVTLVSNFEVKSF 360

Query: 361 DGCANPYLGLAAILCAGLDGLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNN 420
           DGCANPYLGLAAILCAGLDGLR HLQLP+PIDTNP  MGLK QRLPQSLS+ALE LEKNN
Sbjct: 361 DGCANPYLGLAAILCAGLDGLRYHLQLPQPIDTNPSYMGLKFQRLPQSLSEALEHLEKNN 420

Query: 421 TFTDLIGEKLLVAIKTIRKAEVKYYSEHQDAYKQLIQYY 460
           TFTDLIGE+LL AIK IRKAEVKYYS+HQDAYKQLIQ Y
Sbjct: 421 TFTDLIGERLLAAIKEIRKAEVKYYSKHQDAYKQLIQRY 439

BLAST of HG10017032 vs. TAIR 10
Match: AT3G53180.1 (glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases )

HSP 1 Score: 589.3 bits (1518), Expect = 2.6e-168
Identity = 276/440 (62.73%), Postives = 348/440 (79.09%), Query Frame = 0

Query: 21  DVVQKDTKLVRIIWVDASGLHRCRIIPSMRFNDVVKKSGIGLSVSCMARPSFSDIPAGRN 80
           DV +  +  VRIIWVD SG  RCR + + RFN  VKK+G+GL+ + M   SF+D PA  +
Sbjct: 413 DVQEDSSSFVRIIWVDTSGQQRCRAVQAQRFNRSVKKNGVGLTFASMGMTSFTDGPAEES 472

Query: 81  -LTGVGEMRLLPDLSTKRTVPWREQEEMVMGDMYIRPGESWEYCPREALRRVSRILKNEF 140
            LTGVGE+RL+PDLSTK+T+PW +QE MV+ DM ++PGE+W YCPRE LRRV+++LK+EF
Sbjct: 473 KLTGVGEIRLVPDLSTKQTIPWTKQESMVLADMQLKPGEAWGYCPRETLRRVAKVLKDEF 532

Query: 141 DLEMKAGFEIEFLLLKKAVKDGKEDWVPFDSSFYCSASSYDAVSPFFHDVVASLNSLNII 200
           DL M AGFE EF LLK  V++GKE+++PFD   YC+ SS+DA SP FHD+V +L SLNI 
Sbjct: 533 DLVMNAGFENEFYLLKNVVREGKEEYMPFDFGPYCATSSFDAASPIFHDIVPALESLNIE 592

Query: 201 VEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAMDDMGS 260
           VEQ HAE G GQFE +LG+T    AAD L+YTREVI+++ARK GLLATF+PKY   D+GS
Sbjct: 593 VEQFHAESGKGQFEVSLGHTIASHAADNLVYTREVIRSVARKQGLLATFVPKYDYCDIGS 652

Query: 261 GSHVHISLWRNGKNVLMAADKSSKHGMSTIGEEFMAGVFNHITSILPFIAPVPNSYDRIQ 320
           GSHVH+SLW+NG+NV  A++ SS HG+S++GEEFMAGV  H+ SIL  IAP+PNSYDRIQ
Sbjct: 653 GSHVHLSLWKNGENVFPASNNSSSHGISSVGEEFMAGVLFHLPSILAIIAPLPNSYDRIQ 712

Query: 321 PNTWSGAYHCWGKDNKEAPIRTASPPGIGVSLVSNFEVKSFDGCANPYLGLAAILCAGLD 380
           PNTWSGA+ CWGK+N+EA +R ASPPG    LV+NFE+KSFDG ANP+LGLA I+ AG+D
Sbjct: 713 PNTWSGAFQCWGKENREAALRAASPPGTPDGLVTNFEIKSFDGSANPHLGLAVIMAAGID 772

Query: 381 GLRNHLQLPEPIDTNPFGMGLKLQRLPQSLSDALEALEKNNTFTDLIGEKLLVAIKTIRK 440
           GLR HLQLP PID NP  +   L RLP++LS+A+EAL+K+    DL+G+KLLVAIK +RK
Sbjct: 773 GLRRHLQLPTPIDINPADVAATLNRLPETLSEAVEALDKDKVLHDLLGQKLLVAIKGVRK 832

Query: 441 AEVKYYSEHQDAYKQLIQYY 460
           AEV+YYS++ DAYKQLI  Y
Sbjct: 833 AEVEYYSKNPDAYKQLIHRY 852

BLAST of HG10017032 vs. TAIR 10
Match: AT5G37600.1 (glutamine synthase clone R1 )

HSP 1 Score: 50.8 bits (120), Expect = 3.3e-06
Identity = 71/270 (26.30%), Postives = 107/270 (39.63%), Query Frame = 0

Query: 15  DANNLGDVVQKDTKLVRIIWVDASGL---HRCRIIPSMRFNDVVKKSGIGLSVSCMARPS 74
           D  NL      D  +   IWV  SG+    + R +P           G     S + + +
Sbjct: 6   DLINLNLSDSTDKIIAEYIWVGGSGMDMRSKARTLP-----------GPVTDPSQLPKWN 65

Query: 75  FSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEE-MVMGDMYIRPGESWEYCPREALRR 134
           +     G+      E+ L P    K   P+R     +VM D Y   GE     P      
Sbjct: 66  YDGSSTGQAPGEDSEVILYPQAIFKD--PFRRGNNILVMCDAYTPAGEP---IPTNKRHA 125

Query: 135 VSRILKNEFDLEMKA---GFEIEFLLLKKAVKDGKEDWVPF------DSSFYCSASSYDA 194
            +++  N  D+  +    G E E+ LL+K VK     W P          +YC      A
Sbjct: 126 AAKVFSNP-DVAAEVPWYGIEQEYTLLQKDVK-WPVGW-PIGGYPGPQGPYYCGIG---A 185

Query: 195 VSPFFHDVVASLNS----LNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKA 254
              F  DVV S         I +  ++ EV  GQ+EF +G    +SAADE+   R +++ 
Sbjct: 186 DKSFGRDVVDSHYKACLYAGINISGINGEVMPGQWEFQVGPAVGISAADEIWVARYILER 245

Query: 255 IARKHGLLATFIPKYAMDDM-GSGSHVHIS 267
           I    G++ +F PK    D  G+G+H + S
Sbjct: 246 ITEIAGVVVSFDPKPIPGDWNGAGAHCNYS 253

BLAST of HG10017032 vs. TAIR 10
Match: AT5G16570.1 (glutamine synthetase 1;4 )

HSP 1 Score: 45.8 bits (107), Expect = 1.1e-04
Identity = 66/268 (24.63%), Postives = 106/268 (39.55%), Query Frame = 0

Query: 15  DANNLGDVVQKDTKLVRIIWVDASGL---HRCRIIPSMRFNDVVKKSGIGLSVSCMARPS 74
           D  NL      D  +   IW+  SGL    + R +P           G     S + + +
Sbjct: 6   DLINLDLSDSTDQIIAEYIWIGGSGLDMRSKARTLP-----------GPVTDPSQLPKWN 65

Query: 75  FSDIPAGRNLTGVGEMRLLPDLSTKRTVPWREQEE-MVMGDMYIRPGESWEYCPREALRR 134
           +     G+      E+ + P    K   P+R     +VM D Y   GE     P      
Sbjct: 66  YDGSSTGQAPGDDSEVIIYPQAIFKD--PFRRGNNILVMCDAYTPAGEP---IPTNKRHA 125

Query: 135 VSRILKNEFDL--EMKAGFEIEFLLLKKAVKDGKEDWVPF-----DSSFYCSASSYDAVS 194
            ++I ++   +  E   G E E+ LL+K +K     W           +YC   +  A  
Sbjct: 126 AAKIFEDPSVVAEETWYGIEQEYTLLQKDIK-WPVGWPVGGFPGPQGPYYCGVGADKA-- 185

Query: 195 PFFHDVVASLNS----LNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIA 254
            F  D+V S         I V   + EV  GQ+EF +G T  ++AAD++   R +++ I 
Sbjct: 186 -FGRDIVDSHYKACLYAGINVSGTNGEVMPGQWEFQVGPTVGIAAADQVWVARYILERIT 245

Query: 255 RKHGLLATFIPKYAMDDM-GSGSHVHIS 267
              G++ +  PK    D  G+G+H + S
Sbjct: 246 ELAGVVLSLDPKPIPGDWNGAGAHTNYS 253

BLAST of HG10017032 vs. TAIR 10
Match: AT1G66200.1 (glutamine synthase clone F11 )

HSP 1 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 43/133 (32.33%), Postives = 66/133 (49.62%), Query Frame = 0

Query: 146 GFEIEFLLLKKAVKDGKEDWVPF------DSSFYCSASSYDAVSPFFHDVV-----ASLN 205
           G E E+ LL+K V +    W P          +YCS     A   F  D+V     ASL 
Sbjct: 127 GIEQEYTLLQKDV-NWPLGW-PIGGFPGPQGPYYCSIG---ADKSFGRDIVDAHYKASLY 186

Query: 206 SLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAM 265
           +  I +  ++ EV  GQ+EF +G +  +SAADE+   R +++ I    G++ +F PK   
Sbjct: 187 A-GINISGINGEVMPGQWEFQVGPSVGISAADEIWIARYILERITEIAGVVVSFDPKPIP 246

Query: 266 DDM-GSGSHVHIS 267
            D  G+G+H + S
Sbjct: 247 GDWNGAGAHTNYS 253

BLAST of HG10017032 vs. TAIR 10
Match: AT1G66200.3 (glutamine synthase clone F11 )

HSP 1 Score: 45.4 bits (106), Expect = 1.4e-04
Identity = 43/133 (32.33%), Postives = 66/133 (49.62%), Query Frame = 0

Query: 146 GFEIEFLLLKKAVKDGKEDWVPF------DSSFYCSASSYDAVSPFFHDVV-----ASLN 205
           G E E+ LL+K V +    W P          +YCS     A   F  D+V     ASL 
Sbjct: 136 GIEQEYTLLQKDV-NWPLGW-PIGGFPGPQGPYYCSIG---ADKSFGRDIVDAHYKASLY 195

Query: 206 SLNIIVEQLHAEVGNGQFEFALGYTSCLSAADELIYTREVIKAIARKHGLLATFIPKYAM 265
           +  I +  ++ EV  GQ+EF +G +  +SAADE+   R +++ I    G++ +F PK   
Sbjct: 196 A-GINISGINGEVMPGQWEFQVGPSVGISAADEIWIARYILERITEIAGVVVSFDPKPIP 255

Query: 266 DDM-GSGSHVHIS 267
            D  G+G+H + S
Sbjct: 256 GDWNGAGAHTNYS 262

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK07337.13.6e-22884.31type-1 glutamine synthetase 1-like [Cucumis melo var. makuwa][more]
XP_008462507.19.7e-22683.66PREDICTED: type-1 glutamine synthetase 1-like [Cucumis melo][more]
XP_011657676.11.2e-22385.62type-1 glutamine synthetase 1 [Cucumis sativus][more]
KGN48473.25.2e-21983.33hypothetical protein Csa_004298 [Cucumis sativus][more]
XP_011657677.11.2e-21582.43type-1 glutamine synthetase 1 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q86B005.6e-5932.73Type-1 glutamine synthetase 1 OS=Dictyostelium discoideum OX=44689 GN=glnA1 PE=3... [more]
P380941.7e-5232.37Protein fluG OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / ... [more]
O596489.9e-4832.33Glutamine synthetase OS=Methanococcus maripaludis (strain S2 / LL) OX=267377 GN=... [more]
P211541.7e-4732.52Glutamine synthetase OS=Methanococcus voltae OX=2188 GN=glnA PE=3 SV=1[more]
Q601822.9e-4732.28Glutamine synthetase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2... [more]
Match NameE-valueIdentityDescription
A0A5D3C6271.7e-22884.31Type-1 glutamine synthetase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CIM44.7e-22683.66type-1 glutamine synthetase 1-like OS=Cucumis melo OX=3656 GN=LOC103500842 PE=3 ... [more]
A0A0A0KES45.7e-22485.62Gln-synt_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G448140 ... [more]
A0A0A0KKB45.8e-21682.43Gln-synt_C domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G448150 ... [more]
A0A5D3CAL16.0e-21380.39Type-1 glutamine synthetase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
Match NameE-valueIdentityDescription
AT3G53180.12.6e-16862.73glutamate-ammonia ligases;catalytics;glutamate-ammonia ligases [more]
AT5G37600.13.3e-0626.30glutamine synthase clone R1 [more]
AT5G16570.11.1e-0424.63glutamine synthetase 1;4 [more]
AT1G66200.11.4e-0432.33glutamine synthase clone F11 [more]
AT1G66200.31.4e-0432.33glutamine synthase clone F11 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008146Glutamine synthetase, catalytic domainSMARTSM01230Gln_synt_C_2coord: 121..377
e-value: 2.5E-84
score: 296.2
IPR008146Glutamine synthetase, catalytic domainPFAMPF00120Gln-synt_Ccoord: 122..451
e-value: 1.9E-90
score: 303.4
IPR036651Glutamine synthetase, N-terminal domain superfamilyGENE3D3.10.20.70coord: 22..117
e-value: 1.4E-25
score: 91.4
IPR036651Glutamine synthetase, N-terminal domain superfamilySUPERFAMILY54368Glutamine synthetase, N-terminal domaincoord: 23..120
NoneNo IPR availableGENE3D3.30.590.10Glutamine synthetase/guanido kinase, catalytic domaincoord: 124..445
e-value: 1.6E-104
score: 351.6
NoneNo IPR availablePANTHERPTHR43785GAMMA-GLUTAMYLPUTRESCINE SYNTHETASEcoord: 25..455
IPR014746Glutamine synthetase/guanido kinase, catalytic domainSUPERFAMILY55931Glutamine synthetase/guanido kinasecoord: 121..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10017032.1HG10017032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006542 glutamine biosynthetic process
biological_process GO:0006807 nitrogen compound metabolic process
molecular_function GO:0004356 glutamate-ammonia ligase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0003824 catalytic activity