HG10016919 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10016919
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	gibberellin 20 oxidase 2-like
Location	Chr03: 9322303 .. 9324432 (-)
RNA-Seq Expression	HG10016919
Synteny	HG10016919

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATCCACCGGCACGACGGCGGCCGGAATTCTCCCGACGATGTTGGACAACAAGAAGAATCTCCCAAGGGAATTTGTGTGGCCACGTGGGGAGTTGGCAGCGGGAAGTGCGGAGGAGCGAGAGGAGCTGAAAGAGCCGTTGATTGACTTGGGGGGATTCCGGCGAGGGGAGGCGGCGGCGACGGCGGAAGCGGCGGCGTTGGTGCGAATGGCGTGTAGGAAGCACGGGCTGTTTCAGGTGACGAATCATGGAGTGGCGGCGGAGCTGATAAAGGCGGCGTATGAAGAAGGAGAAGCGATATTCAAGATGCCATTAGTGAAGAAAATTAGCGTGGGGAAAAAGCCAGGGAGAGTTTCGGGATATTCAGGAGCTCATGCAGAGAGATTCTCGTCAAAGCTTCCATGGAAAGAGACGTTTTCTTTTGAGTATTCAACTGATGATTCTCAACGCCTTCATGTTCTTCATTATTTTAAATCCTTGTTCGGACGCGATTTCGAGAATACTGGGTCAGTCTTCTCATTAACTATTCTTTTTTTTAGATCGAAATTTTGAGTCACTAACACGTACTATATGTCAGTTGATTTTTTATCGTGATGTTTAATAGATTTTTAAATTTTATCATAGTACTTTACGGTCAGTTCATCTTGTTTGTTAAAGATGTTAGTACGTACGTAATAAGTACACATGTACTAAACTATATGTCGTACTTTTTTTTTCGTTATCGTGTCGTGGTGTTTAATAGATTTTTAAACATTCGTAATTCTTTATGGTTAGTTTCTTAAAAATGCTAGTATATAATAAATGCACAAGTACTAAACTAATGAAGTTGGCACTATATACTTGTTAAATAGACAATTGAGAGTTTATGTCTCATTTGATAATTATTTTTTTTTTTTGTTTTAAAAATTTTGTTTGTTTTTTTTTTTTTTTTTTTTTTTGCCATCTTAATTAAGAAAACATTTAAGTTTATACCTGAATTCCAGAAAAACAAAACCATGTTTTTAAAAACTATATACATTTCTTTCGATTTTCAAAATTTTATTTGCTTTTTTTACAATATTTTTGAAAAGTAGTATATTTATAAGCTAAACTTTTTTTTTTTAAAAAAAACATTTATTAATTAATAAGATTTTGAAGACTTATTAAAAACTTGTGAAAGTACATGAAAAATTAAAAATGAAAAATAGAGTTTAGAAGAAAAATACTTAGAGAGTGTGTTTGGACGAGGAGTAGAGTTGTTTAGTTGTGGGCCCACAAAGTAAGAAGTTAATAAAGCAGAAAATTAATGTTTTTTAGTATTTTGGGACCTACAAACTTCTTGAGCCAAACAAGGAGTGATATTAACAACTCTCCTTCCGCAACTTTAATTCTTTGGACAAAACACCTTCTTAGATGCTATTGCTAGTGGGCTACAACCATTTTTTTTTTTTTTTTTTTTGGATAAAGCTACAATCATTTTTAACCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGATTGTCACGTTGTACTATGTGATTTAGCTACAAAAGCTGGATTGCATGGTTGCAGTTCAACTAGCAGCCAAATATTACTCTACTATTTTAGAACCAATATTAATTAATTGAATTTAATTGAAAATGAAGGTATAATTGATGGTTTTGTGTTTGGTGTTTTTGATATTATGAAGATGGGTATACCAAAGATACTGTGAGGAAATGACAAGAACAGCACTAATGATAATGGAGCTTCTAGCAATAAGTTTGGGAGTAGAGAGATGTCATTACAGGAAGTTCTTTGAAGATGGAAGATCAATAATGAGATGCAATTACTATCCACCATGTGAAAATGCAGGGCTGACCTTAGGTACAGGCCCTCATTGTGACCCAACTTCACTCACAATTCTCCATCAAGATCAAGTTGGAGGCCTTGAGGTTTTTGCTAACAATAGATGGCTCTCTGTCAAACCTAGACCAGATGCTTTGGTTATTAACATTGGAGACACTTTTATGGTACATAACTTTCACTCTTACCTTCTTCCTATTATTGTTATTCTATTCTATATGTTTCAAAACGACACGACGGAGGCCCAATTGGGATCGTCGGTGTTTCAAAAGACGTTAAGAGTCTGTTTGAAATGA

mRNA sequence

ATGGAATCCACCGGCACGACGGCGGCCGGAATTCTCCCGACGATGTTGGACAACAAGAAGAATCTCCCAAGGGAATTTGTGTGGCCACGTGGGGAGTTGGCAGCGGGAAGTGCGGAGGAGCGAGAGGAGCTGAAAGAGCCGTTGATTGACTTGGGGGGATTCCGGCGAGGGGAGGCGGCGGCGACGGCGGAAGCGGCGGCGTTGGTGCGAATGGCGTGTAGGAAGCACGGGCTGTTTCAGGTGACGAATCATGGAGTGGCGGCGGAGCTGATAAAGGCGGCGTATGAAGAAGGAGAAGCGATATTCAAGATGCCATTAGTGAAGAAAATTAGCGTGGGGAAAAAGCCAGGGAGAGTTTCGGGATATTCAGGAGCTCATGCAGAGAGATTCTCGTCAAAGCTTCCATGGAAAGAGACGTTTTCTTTTGAGTATTCAACTGATGATTCTCAACGCCTTCATGTTCTTCATTATTTTAAATCCTTGTTCGGACGCGATTTCGAGAATACTGGATGGGTATACCAAAGATACTGTGAGGAAATGACAAGAACAGCACTAATGATAATGGAGCTTCTAGCAATAAGTTTGGGAGTAGAGAGATGTCATTACAGGAAGTTCTTTGAAGATGGAAGATCAATAATGAGATGCAATTACTATCCACCATGTGAAAATGCAGGGCTGACCTTAGGTACAGGCCCTCATTGTGACCCAACTTCACTCACAATTCTCCATCAAGATCAAGTTGGAGGCCTTGAGGTTTTTGCTAACAATAGATGGCTCTCTGTCAAACCTAGACCAGATGCTTTGGTTATTAACATTGGAGACACTTTTATGGTACATAACTTTCACTCTTACCTTCTTCCTATTATTGTTATTCTATTCTATATGTTTCAAAACGACACGACGGAGGCCCAATTGGGATCGTCGGTGTTTCAAAAGACGTTAAGAGTCTGTTTGAAATGA

Coding sequence (CDS)

ATGGAATCCACCGGCACGACGGCGGCCGGAATTCTCCCGACGATGTTGGACAACAAGAAGAATCTCCCAAGGGAATTTGTGTGGCCACGTGGGGAGTTGGCAGCGGGAAGTGCGGAGGAGCGAGAGGAGCTGAAAGAGCCGTTGATTGACTTGGGGGGATTCCGGCGAGGGGAGGCGGCGGCGACGGCGGAAGCGGCGGCGTTGGTGCGAATGGCGTGTAGGAAGCACGGGCTGTTTCAGGTGACGAATCATGGAGTGGCGGCGGAGCTGATAAAGGCGGCGTATGAAGAAGGAGAAGCGATATTCAAGATGCCATTAGTGAAGAAAATTAGCGTGGGGAAAAAGCCAGGGAGAGTTTCGGGATATTCAGGAGCTCATGCAGAGAGATTCTCGTCAAAGCTTCCATGGAAAGAGACGTTTTCTTTTGAGTATTCAACTGATGATTCTCAACGCCTTCATGTTCTTCATTATTTTAAATCCTTGTTCGGACGCGATTTCGAGAATACTGGATGGGTATACCAAAGATACTGTGAGGAAATGACAAGAACAGCACTAATGATAATGGAGCTTCTAGCAATAAGTTTGGGAGTAGAGAGATGTCATTACAGGAAGTTCTTTGAAGATGGAAGATCAATAATGAGATGCAATTACTATCCACCATGTGAAAATGCAGGGCTGACCTTAGGTACAGGCCCTCATTGTGACCCAACTTCACTCACAATTCTCCATCAAGATCAAGTTGGAGGCCTTGAGGTTTTTGCTAACAATAGATGGCTCTCTGTCAAACCTAGACCAGATGCTTTGGTTATTAACATTGGAGACACTTTTATGGTACATAACTTTCACTCTTACCTTCTTCCTATTATTGTTATTCTATTCTATATGTTTCAAAACGACACGACGGAGGCCCAATTGGGATCGTCGGTGTTTCAAAAGACGTTAAGAGTCTGTTTGAAATGA

Protein sequence

MESTGTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSYLLPIIVILFYMFQNDTTEAQLGSSVFQKTLRVCLK

Homology

[Close]

BLAST of HG10016919 vs. NCBI nr
Match: XP_038882991.1 (gibberellin 20 oxidase 1-D-like [Benincasa hispida])

HSP 1 Score: 549.3 bits (1414), Expect = 2.2e-152
Identity = 265/284 (93.31%), Postives = 273/284 (96.13%), Query Frame = 0


Query: 1   MESTGTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAA 60
           MESTGTTA  ILPTMLDN+KNLP EF+WPRGELA G+AEEREELKEPLIDLGGFRRGEAA
Sbjct: 1   MESTGTTAGVILPTMLDNQKNLPMEFIWPRGELATGNAEEREELKEPLIDLGGFRRGEAA 60

Query: 61  ATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS 120
           ATAEAAA+VRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS
Sbjct: 61  ATAEAAAMVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS 120

Query: 121 GYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEM 180
           GYSGAHAERFSSKLPWKETFSFEY +DDSQRLHVLHYFKSLFG DFENTGWVYQRYCEEM
Sbjct: 121 GYSGAHAERFSSKLPWKETFSFEYCSDDSQRLHVLHYFKSLFGCDFENTGWVYQRYCEEM 180

Query: 181 TRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT 240
           TRTALMIMELLAISLG+ER HYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT
Sbjct: 181 TRTALMIMELLAISLGIERFHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT 240

Query: 241 ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMALSNGAY 284

[Close]

BLAST of HG10016919 vs. NCBI nr
Match: XP_004143402.2 (gibberellin 20 oxidase 2 [Cucumis sativus] >QRH15959.1 LH2, partial [Cucumis sativus] >CCD28480.1 GA 20-oxidase [Cucumis sativus])

HSP 1 Score: 502.7 bits (1293), Expect = 2.4e-138
Identity = 249/287 (86.76%), Postives = 257/287 (89.55%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EER ELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEERGELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VRMAC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYC 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDSQ LHVLH+FKSLFG DFENTGWVYQRYC
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYC 180

Query: 181 EEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPT 240
           EEMTRTALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDPT
Sbjct: 181 EEMTRTALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPT 240

Query: 241 SLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           SLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 SLTILHQDQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAY 284

[Close]

BLAST of HG10016919 vs. NCBI nr
Match: KAE8647437.1 (hypothetical protein Csa_002924 [Cucumis sativus])

HSP 1 Score: 502.7 bits (1293), Expect = 2.4e-138
Identity = 249/287 (86.76%), Postives = 257/287 (89.55%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EER ELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEERGELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VRMAC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYC 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDSQ LHVLH+FKSLFG DFENTGWVYQRYC
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYC 180

Query: 181 EEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPT 240
           EEMTRTALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDPT
Sbjct: 181 EEMTRTALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPT 240

Query: 241 SLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           SLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 SLTILHQDQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAY 284

[Close]

BLAST of HG10016919 vs. NCBI nr
Match: XP_008462644.1 (PREDICTED: gibberellin 20 oxidase 2-like [Cucumis melo])

HSP 1 Score: 498.8 bits (1283), Expect = 3.4e-137
Identity = 249/288 (86.46%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EEREELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEEREELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VR+AC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRVACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDS-QRLHVLHYFKSLFGRDFENTGWVYQRY 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDS Q LHVLHYFKSLFG DFENTGWVYQRY
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSDQPLHVLHYFKSLFGCDFENTGWVYQRY 180

Query: 181 CEEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDP 240
           CEEMTR ALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDP
Sbjct: 181 CEEMTRIALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDP 240

Query: 241 TSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           TSLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 TSLTILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAY 285

[Close]

BLAST of HG10016919 vs. NCBI nr
Match: KAA0025201.1 (gibberellin 20 oxidase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 498.8 bits (1283), Expect = 3.4e-137
Identity = 249/288 (86.46%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EEREELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEEREELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VR+AC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRVACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDS-QRLHVLHYFKSLFGRDFENTGWVYQRY 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDS Q LHVLHYFKSLFG DFENTGWVYQRY
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSDQPLHVLHYFKSLFGCDFENTGWVYQRY 180

Query: 181 CEEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDP 240
           CEEMTR ALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDP
Sbjct: 181 CEEMTRIALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDP 240

Query: 241 TSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           TSLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 TSLTILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAY 285

[Close]

BLAST of HG10016919 vs. ExPASy Swiss-Prot
Match: O04706 (Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-74
Identity = 138/263 (52.47%), Postives = 171/263 (65.02%), Query Frame = 0


Query: 15  MLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMACR 74
           +L  + ++P +F+WP GE  + + +  EEL  PLID+GG   G+  ATAE   LV  AC 
Sbjct: 10  VLSGRADIPSQFIWPEGE--SPTPDAAEELHVPLIDIGGMLSGDPRATAEVTRLVGEACE 69

Query: 75  KHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSKL 134
           +HG FQV NHG+ AEL+  A+   +A F MPL +K    ++PG   GY+ +   RF+SKL
Sbjct: 70  RHGFFQVVNHGIDAELLADAHRCVDAFFTMPLPEKQRALRRPGESCGYASSFTGRFASKL 129

Query: 135 PWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLAIS 194
           PWKET SF     D     V+ Y  +  G D    G VY RYC EM+R +L IME+L  S
Sbjct: 130 PWKETLSFRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGES 189

Query: 195 LGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFA 254
           LGV R HYR+FFE   SIMR NYYPPC+    TLGTGPHCDPTSLTILHQD VGGL+V  
Sbjct: 190 LGVGRAHYRRFFEGNDSIMRLNYYPPCQRPMETLGTGPHCDPTSLTILHQDNVGGLQVHT 249

Query: 255 NNRWLSVKPRPDALVINIGDTFM 278
             RW S++PR DA V+NIGDTFM
Sbjct: 250 EGRWRSIRPRADAFVVNIGDTFM 268

[Close]

BLAST of HG10016919 vs. ExPASy Swiss-Prot
Match: P0C5H5 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-74
Identity = 144/270 (53.33%), Postives = 173/270 (64.07%), Query Frame = 0


Query: 8   AAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAA 67
           AA +    ++ K  +P  FVWP G+    SA    EL  P++D+G  R G+A     AAA
Sbjct: 30  AAAVCDLRMEPK--IPEPFVWPNGDARPASA---AELDMPVVDVGVLRDGDAEGLRRAAA 89

Query: 68  LVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHA 127
            V  AC  HG FQV+ HGV A L +AA +     F++PL +K    + PG VSGY+ AHA
Sbjct: 90  QVAAACATHGFFQVSEHGVDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHA 149

Query: 128 ERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMI 187
           +RF+SKLPWKET SF +  D +    V  YF S  G DF   G VYQ+YCEEM   +L I
Sbjct: 150 DRFASKLPWKETLSFGFH-DRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTI 209

Query: 188 MELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQV 247
           MELL +SLGVER +YR+FF D  SIMRCNYYPPC     TLGTGPHCDPT+LTIL QD V
Sbjct: 210 MELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDV 269

Query: 248 GGLEVFANNRWLSVKPRPDALVINIGDTFM 278
           GGLEV  +  W  V P P A+VINIGDTFM
Sbjct: 270 GGLEVLVDGEWRPVSPVPGAMVINIGDTFM 293

[Close]

BLAST of HG10016919 vs. ExPASy Swiss-Prot
Match: Q0JH50 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA20OX2 PE=2 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.9e-74
Identity = 144/270 (53.33%), Postives = 173/270 (64.07%), Query Frame = 0


Query: 8   AAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAA 67
           AA +    ++ K  +P  FVWP G+    SA    EL  P++D+G  R G+A     AAA
Sbjct: 30  AAAVCDLRMEPK--IPEPFVWPNGDARPASA---AELDMPVVDVGVLRDGDAEGLRRAAA 89

Query: 68  LVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHA 127
            V  AC  HG FQV+ HGV A L +AA +     F++PL +K    + PG VSGY+ AHA
Sbjct: 90  QVAAACATHGFFQVSEHGVDAALARAALDGASDFFRLPLAEKRRARRVPGTVSGYTSAHA 149

Query: 128 ERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMI 187
           +RF+SKLPWKET SF +  D +    V  YF S  G DF   G VYQ+YCEEM   +L I
Sbjct: 150 DRFASKLPWKETLSFGFH-DRAAAPVVADYFSSTLGPDFAPMGRVYQKYCEEMKELSLTI 209

Query: 188 MELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQV 247
           MELL +SLGVER +YR+FF D  SIMRCNYYPPC     TLGTGPHCDPT+LTIL QD V
Sbjct: 210 MELLELSLGVERGYYREFFADSSSIMRCNYYPPCPEPERTLGTGPHCDPTALTILLQDDV 269

Query: 248 GGLEVFANNRWLSVKPRPDALVINIGDTFM 278
           GGLEV  +  W  V P P A+VINIGDTFM
Sbjct: 270 GGLEVLVDGEWRPVSPVPGAMVINIGDTFM 293

[Close]

BLAST of HG10016919 vs. ExPASy Swiss-Prot
Match: O04707 (Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 9.6e-74
Identity = 137/263 (52.09%), Postives = 171/263 (65.02%), Query Frame = 0


Query: 15  MLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMACR 74
           +L  + ++P +F+WP GE  + + +  EEL  PLI++GG   G+AAA AE   LV  AC 
Sbjct: 10  VLSGRSDIPSQFIWPEGE--SPTPDAAEELHVPLINIGGMLSGDAAAAAEVTRLVGEACE 69

Query: 75  KHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSKL 134
           +HG FQV NHG+ AEL+  A+   +  F MPL +K    ++PG   GY+ +   RF+SKL
Sbjct: 70  RHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRRPGESCGYASSFTGRFASKL 129

Query: 135 PWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLAIS 194
           PWKET SF     D     V+ Y  +  G D    G VY RYC EM+R +L IME+L  S
Sbjct: 130 PWKETLSFRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGES 189

Query: 195 LGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFA 254
           LGV R HYR+FFE   SIMR NYYPPC+    TLGTGPHCDPTSLTILHQD VGGL+V  
Sbjct: 190 LGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHT 249

Query: 255 NNRWLSVKPRPDALVINIGDTFM 278
             RW S++PR DA V+NIGDTFM
Sbjct: 250 EGRWRSIRPRADAFVVNIGDTFM 268

[Close]

BLAST of HG10016919 vs. ExPASy Swiss-Prot
Match: O04705 (Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.8e-73
Identity = 137/263 (52.09%), Postives = 169/263 (64.26%), Query Frame = 0


Query: 15  MLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMACR 74
           +L  + ++P +F+WP GE  + + +  EEL  PLID+GG   G+ AA AE   LV  AC 
Sbjct: 10  VLSGRADIPSQFIWPEGE--SPTPDAAEELHVPLIDIGGMLSGDPAAAAEVTRLVGEACE 69

Query: 75  KHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSKL 134
           +HG FQV NHG+ AEL+  A+   +  F MPL +K    + PG   GY+ +   RF+SKL
Sbjct: 70  RHGFFQVVNHGIDAELLADAHRCVDNFFTMPLPEKQRALRHPGESCGYASSFTGRFASKL 129

Query: 135 PWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLAIS 194
           PWKET SF     D     V+ Y  +  G D    G VY RYC EM+R +L IME+L  S
Sbjct: 130 PWKETLSFRSCPSDPAL--VVDYIVATLGEDHRRLGEVYARYCSEMSRLSLEIMEVLGES 189

Query: 195 LGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFA 254
           LGV R HYR+FFE   SIMR NYYPPC+    TLGTGPHCDPTSLTILHQD VGGL+V  
Sbjct: 190 LGVGRAHYRRFFEGNDSIMRLNYYPPCQRPLETLGTGPHCDPTSLTILHQDNVGGLQVHT 249

Query: 255 NNRWLSVKPRPDALVINIGDTFM 278
             RW S++PR DA V+NIGDTFM
Sbjct: 250 EGRWRSIRPRADAFVVNIGDTFM 268

[Close]

BLAST of HG10016919 vs. ExPASy TrEMBL
Match: S0BDZ8 (GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox2 PE=2 SV=1)

HSP 1 Score: 502.7 bits (1293), Expect = 1.2e-138
Identity = 249/287 (86.76%), Postives = 257/287 (89.55%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EER ELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEERGELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VRMAC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRMACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYC 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDSQ LHVLH+FKSLFG DFENTGWVYQRYC
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSQPLHVLHHFKSLFGCDFENTGWVYQRYC 180

Query: 181 EEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPT 240
           EEMTRTALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDPT
Sbjct: 181 EEMTRTALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDPT 240

Query: 241 SLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           SLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 SLTILHQDQVGGLEVFANNAWLSVKPRPDALVINIGDTFMALSNGAY 284

[Close]

BLAST of HG10016919 vs. ExPASy TrEMBL
Match: A0A5A7SLS2 (Gibberellin 20 oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G00470 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.7e-137
Identity = 249/288 (86.46%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EEREELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEEREELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VR+AC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRVACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDS-QRLHVLHYFKSLFGRDFENTGWVYQRY 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDS Q LHVLHYFKSLFG DFENTGWVYQRY
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSDQPLHVLHYFKSLFGCDFENTGWVYQRY 180

Query: 181 CEEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDP 240
           CEEMTR ALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDP
Sbjct: 181 CEEMTRIALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDP 240

Query: 241 TSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           TSLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 TSLTILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAY 285

[Close]

BLAST of HG10016919 vs. ExPASy TrEMBL
Match: A0A1S3CJ08 (gibberellin 20 oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103500953 PE=3 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.7e-137
Identity = 249/288 (86.46%), Postives = 257/288 (89.24%), Query Frame = 0


Query: 1   MEST---GTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRG 60
           MEST   G  A  ILPTM+D KK LPREFVWPRGELA    EEREELKEPLIDLGGFRRG
Sbjct: 1   MESTTTVGRVAGIILPTMVDKKKKLPREFVWPRGELA---GEEREELKEPLIDLGGFRRG 60

Query: 61  EAAATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPG 120
           E  ATAEAAA+VR+AC KHG+FQVTNHGV  ELIKAAYEEGE IFKMPLVKKISVGKKPG
Sbjct: 61  EEEATAEAAAMVRVACMKHGVFQVTNHGVEEELIKAAYEEGEGIFKMPLVKKISVGKKPG 120

Query: 121 RVSGYSGAHAERFSSKLPWKETFSFEYSTDDS-QRLHVLHYFKSLFGRDFENTGWVYQRY 180
           RVSGYSGAHA+RFSSKLPWKETFSFEYS DDS Q LHVLHYFKSLFG DFENTGWVYQRY
Sbjct: 121 RVSGYSGAHADRFSSKLPWKETFSFEYSNDDSDQPLHVLHYFKSLFGCDFENTGWVYQRY 180

Query: 181 CEEMTRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDP 240
           CEEMTR ALMIMELLAISLGVER HYRKFFEDG+SIMRCNYYPPCENA LTLGTGPHCDP
Sbjct: 181 CEEMTRIALMIMELLAISLGVERYHYRKFFEDGKSIMRCNYYPPCENASLTLGTGPHCDP 240

Query: 241 TSLTILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           TSLTILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 TSLTILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAY 285

[Close]

BLAST of HG10016919 vs. ExPASy TrEMBL
Match: A0A6J1EG31 (gibberellin 20 oxidase 1-D-like OS=Cucurbita moschata OX=3662 GN=LOC111433062 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.9e-126
Identity = 227/284 (79.93%), Postives = 247/284 (86.97%), Query Frame = 0


Query: 1   MESTGTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAA 60
           M+STGT  A I PT+L N  NLP EFVWP  +LAA   ++ EEL+EPLIDL GFRRG+AA
Sbjct: 1   MDSTGT--AVIPPTLLHN-GNLPTEFVWPHADLAA-IVDDPEELQEPLIDLAGFRRGDAA 60

Query: 61  ATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS 120
           ATA+AAA++R+ACR HGLFQVTNHGV  +LI+AAYE+ E IFKMPL KK SVGKK GRV 
Sbjct: 61  ATADAAAMIRVACRNHGLFQVTNHGVDPDLIRAAYEQVEMIFKMPLGKKKSVGKKAGRVC 120

Query: 121 GYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEM 180
           GYSGAHA+RFSSKLPWKETFSFEY +DDSQRLHVLHYFKS  G+DFE TGWVYQ+YCEEM
Sbjct: 121 GYSGAHADRFSSKLPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEM 180

Query: 181 TRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT 240
           TRT LMIMELLAISLGVER HYRKFFEDGRSIMRCNYYPPCENA LT GTGPHCDPTSLT
Sbjct: 181 TRTGLMIMELLAISLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLT 240

Query: 241 ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           ILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 ILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAY 280

[Close]

BLAST of HG10016919 vs. ExPASy TrEMBL
Match: A0A6J1ITP8 (gibberellin 20 oxidase 1-D-like OS=Cucurbita maxima OX=3661 GN=LOC111478538 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 3.8e-126
Identity = 224/284 (78.87%), Postives = 245/284 (86.27%), Query Frame = 0


Query: 1   MESTGTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAA 60
           M+STGT A   +P  L +  NLP EFVWP  +LAA   ++ EEL+EPLIDL GFRRG+AA
Sbjct: 1   MDSTGTAA---IPPTLFHNGNLPTEFVWPHADLAA-IVDDPEELQEPLIDLAGFRRGDAA 60

Query: 61  ATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS 120
           ATA+AAA++R+ACR HGLFQVTNHGV  +LI+AAYE+ E IFKMPL KK SVGKK GRV 
Sbjct: 61  ATADAAAMIRVACRNHGLFQVTNHGVDPDLIRAAYEQVETIFKMPLGKKKSVGKKAGRVC 120

Query: 121 GYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEM 180
           GYSGAHA+RFSSKLPWKETFSFEY +DDSQRLHVLHYFKS  G+DFE TGWVYQ+YCEEM
Sbjct: 121 GYSGAHADRFSSKLPWKETFSFEYCSDDSQRLHVLHYFKSFLGQDFEKTGWVYQKYCEEM 180

Query: 181 TRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT 240
           TRT LMIMELLAISLGVER HYRKFFEDGRSIMRCNYYPPCENA LT GTGPHCDPTSLT
Sbjct: 181 TRTGLMIMELLAISLGVERLHYRKFFEDGRSIMRCNYYPPCENAALTFGTGPHCDPTSLT 240

Query: 241 ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           ILHQDQVGGLEVFANN WLSVKPRPDALVINIGDTFM  +  +Y
Sbjct: 241 ILHQDQVGGLEVFANNTWLSVKPRPDALVINIGDTFMALSNGAY 280

[Close]

BLAST of HG10016919 vs. TAIR 10
Match: AT4G25420.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 4.9e-73
Identity = 135/265 (50.94%), Postives = 174/265 (65.66%), Query Frame = 0


Query: 13  PTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMA 72
           P+ML+ + N+P +F+WP  E  + +     EL  PLIDL      + ++T +A+ L+  A
Sbjct: 31  PSMLNLQANIPNQFIWPDDEKPSINV---LELDVPLIDLQNL-LSDPSSTLDASRLISEA 90

Query: 73  CRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSS 132
           C+KHG F V NHG++ ELI  A+E     F MPL +K  V +K G   GY+ +   RFS+
Sbjct: 91  CKKHGFFLVVNHGISEELISDAHEYTSRFFDMPLSEKQRVLRKSGESVGYASSFTGRFST 150

Query: 133 KLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLA 192
           KLPWKET SF +  D S+   V  YF    G  F+  G VYQ YCE M+  +L IMELL 
Sbjct: 151 KLPWKETLSFRFCDDMSRSKSVQDYFCDALGHGFQPFGKVYQEYCEAMSSLSLKIMELLG 210

Query: 193 ISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEV 252
           +SLGV+R ++R+FFE+  SIMR NYYPPC    LTLGTGPHCDPTSLTILHQD V GL+V
Sbjct: 211 LSLGVKRDYFREFFEENDSIMRLNYYPPCIKPDLTLGTGPHCDPTSLTILHQDHVNGLQV 270

Query: 253 FANNRWLSVKPRPDALVINIGDTFM 278
           F  N+W S++P P A V+NIGDTFM
Sbjct: 271 FVENQWRSIRPNPKAFVVNIGDTFM 291

[Close]

BLAST of HG10016919 vs. TAIR 10
Match: AT5G51810.1 (gibberellin 20 oxidase 2 )

HSP 1 Score: 269.2 bits (687), Expect = 4.1e-72
Identity = 134/277 (48.38%), Postives = 175/277 (63.18%), Query Frame = 0


Query: 1   MESTGTTAAGILPTMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAA 60
           +E   T+     P++L+ +  +P +F+WP  E     + +  EL  P IDL         
Sbjct: 21  LEKDQTSPLIFNPSLLNLQSQIPNQFIWPDEE---KPSIDIPELNVPFIDL-----SSQD 80

Query: 61  ATAEAAALVRMACRKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVS 120
           +T EA  ++  AC KHG F V NHGV+  LI  A+   E+ F MPL  K    +KPG   
Sbjct: 81  STLEAPRVIAEACTKHGFFLVVNHGVSESLIADAHRLMESFFDMPLAGKQKAQRKPGESC 140

Query: 121 GYSGAHAERFSSKLPWKETFSFEYSTDDSQRLHVLHYFKSLFGRDFENTGWVYQRYCEEM 180
           GY+ +   RFS+KLPWKET SF++S D+S    V  YF    G++FE  G VYQ YCE M
Sbjct: 141 GYASSFTGRFSTKLPWKETLSFQFSNDNSGSRTVQDYFSDTLGQEFEQFGKVYQDYCEAM 200

Query: 181 TRTALMIMELLAISLGVERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLT 240
           +  +L IMELL +SLGV R ++R FFE+  SIMR N+YPPC+   LTLGTGPHCDP+SLT
Sbjct: 201 SSLSLKIMELLGLSLGVNRDYFRGFFEENDSIMRLNHYPPCQTPDLTLGTGPHCDPSSLT 260

Query: 241 ILHQDQVGGLEVFANNRWLSVKPRPDALVINIGDTFM 278
           ILHQD V GL+VF +N+W S++P P A V+NIGDTFM
Sbjct: 261 ILHQDHVNGLQVFVDNQWQSIRPNPKAFVVNIGDTFM 289

[Close]

BLAST of HG10016919 vs. TAIR 10
Match: AT5G07200.1 (gibberellin 20-oxidase 3 )

HSP 1 Score: 260.8 bits (665), Expect = 1.5e-69
Identity = 130/260 (50.00%), Postives = 170/260 (65.38%), Query Frame = 0


Query: 21  NLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMACRKHGLFQ 80
           ++P++FVWP  E     + + + L+ PLIDL GF  G++   +EA  LV  A  KHG F 
Sbjct: 36  HIPQQFVWPDHE---KPSTDVQPLQVPLIDLAGFLSGDSCLASEATRLVSKAATKHGFFL 95

Query: 81  VTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSKLPWKETF 140
           +TNHGV   L+  AY   ++ FK P  +K    +K G  SGY+ +   RFSSKLPWKET 
Sbjct: 96  ITNHGVDESLLSRAYLHMDSFFKAPACEKQKAQRKWGESSGYASSFVGRFSSKLPWKETL 155

Query: 141 SFEYSTDDSQRLH---VLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLAISLGV 200
           SF++S +  +++H   V  +     G  +E+ G VYQ Y E M   +L IMELL +SLGV
Sbjct: 156 SFKFSPE--EKIHSQTVKDFVSKKMGDGYEDFGKVYQEYAEAMNTLSLKIMELLGMSLGV 215

Query: 201 ERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFANNR 260
           ER ++++FFED  SI R NYYP C+   L LGTGPHCDPTSLTILHQDQVGGL+VF +N+
Sbjct: 216 ERRYFKEFFEDSDSIFRLNYYPQCKQPELALGTGPHCDPTSLTILHQDQVGGLQVFVDNK 275

Query: 261 WLSVKPRPDALVINIGDTFM 278
           W S+ P P A V+NIGDTFM
Sbjct: 276 WQSIPPNPHAFVVNIGDTFM 290

[Close]

BLAST of HG10016919 vs. TAIR 10
Match: AT1G60980.1 (gibberellin 20-oxidase 4 )

HSP 1 Score: 248.1 bits (632), Expect = 9.8e-66
Identity = 124/260 (47.69%), Postives = 164/260 (63.08%), Query Frame = 0


Query: 21  NLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMACRKHGLFQ 80
           ++P+EFVWP  E  + +      L+ P+IDL GF   +    +EA  LV  A +KHG F 
Sbjct: 35  HIPQEFVWPDHEKPSKNV---PILQVPVIDLAGFLSNDPLLVSEAERLVSEAAKKHGFFL 94

Query: 81  VTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSKLPWKETF 140
           VTNHGV   L+  A++  +  FK P  +K+   +K G  +GY+ +   RF   LPWKET 
Sbjct: 95  VTNHGVDERLLSTAHKLMDTFFKSPNYEKLKAQRKVGETTGYASSFVGRFKENLPWKETL 154

Query: 141 SFEYS-TDDSQRLH--VLHYFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLAISLGV 200
           SF +S T+ S+     V +Y     G  +++ G VYQ Y E M+  +L IMELL +SLG+
Sbjct: 155 SFSFSPTEKSENYSQTVKNYISKTMGDGYKDFGSVYQEYAETMSNLSLKIMELLGMSLGI 214

Query: 201 ERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLEVFANNR 260
           +R H+R+FFED  SI R NYYP C+   L LGTGPHCDPTSLTIL QDQV GL+VF +N+
Sbjct: 215 KREHFREFFEDNESIFRLNYYPKCKQPDLVLGTGPHCDPTSLTILQQDQVSGLQVFVDNQ 274

Query: 261 WLSVKPRPDALVINIGDTFM 278
           W S+ P P ALV+NIGDT M
Sbjct: 275 WQSIPPIPQALVVNIGDTLM 291

[Close]

BLAST of HG10016919 vs. TAIR 10
Match: AT1G44090.1 (gibberellin 20-oxidase 5 )

HSP 1 Score: 233.4 bits (594), Expect = 2.5e-61
Identity = 117/273 (42.86%), Postives = 162/273 (59.34%), Query Frame = 0


Query: 14  TMLDNKKNLPREFVWPRGELAAGSAEEREELKEPLIDLGGFRRGEAAATAEAAALVRMAC 73
           ++L ++ N+P EF WP  ++A        +L  P+IDL GF  G  A T  AA  V+ AC
Sbjct: 35  SLLQSQPNVPAEFFWPEKDVAPSEG----DLDLPIIDLSGFLNGNEAETQLAAKAVKKAC 94

Query: 74  RKHGLFQVTNHGVAAELIKAAYEEGEAIFKMPLVKKISVGKKPGRVSGYSGAHAERFSSK 133
             HG F V NHG  + L + A E     F +   +K+   + PG +SGY+  H++RFSS 
Sbjct: 95  MAHGTFLVVNHGFKSGLAEKALEISSLFFGLSKDEKLRAYRIPGNISGYTAGHSQRFSSN 154

Query: 134 LPWKETFSFEYSTDDSQRLHVLH-YFKSLFGRDFENTGWVYQRYCEEMTRTALMIMELLA 193
           LPW ET +  +        HV+  +  S  G   +  G V+Q +C+ M    + +MELL 
Sbjct: 155 LPWNETLTLAFKKGPP---HVVEDFLTSRLGNHRQEIGQVFQEFCDAMNGLVMDLMELLG 214

Query: 194 ISLGV-ERCHYRKFFEDGRSIMRCNYYPPCENAGLTLGTGPHCDPTSLTILHQDQVGGLE 253
           IS+G+ +R +YR+FFEDG  I RCNYYPPC+     LG GPH DPT++T+L QD V GLE
Sbjct: 215 ISMGLKDRTYYRRFFEDGSGIFRCNYYPPCKQPEKALGVGPHNDPTAITVLLQDDVVGLE 274

Query: 254 VFANNRWLSVKPRPDALVINIGDTFMVHNFHSY 285
           VFA   W +V+PRP ALV+N+GDTFM  +  +Y
Sbjct: 275 VFAAGSWQTVRPRPGALVVNVGDTFMALSNGNY 300

The following BLAST results are available for this feature:

BLAST of HG10016919 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038882991.1	2.2e-152	93.31	gibberellin 20 oxidase 1-D-like [Benincasa hispida]	[more]
XP_004143402.2	2.4e-138	86.76	gibberellin 20 oxidase 2 [Cucumis sativus] >QRH15959.1 LH2, partial [Cucumis sat...	[more]
KAE8647437.1	2.4e-138	86.76	hypothetical protein Csa_002924 [Cucumis sativus]	[more]
XP_008462644.1	3.4e-137	86.46	PREDICTED: gibberellin 20 oxidase 2-like [Cucumis melo]	[more]
KAA0025201.1	3.4e-137	86.46	gibberellin 20 oxidase 2-like [Cucumis melo var. makuwa]	[more]

BLAST of HG10016919 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04706	1.9e-74	52.47	Gibberellin 20 oxidase 1-B OS=Triticum aestivum OX=4565 GN=GA20ox1B PE=2 SV=1	[more]
P0C5H5	1.9e-74	53.33	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV...	[more]
Q0JH50	1.9e-74	53.33	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=GA20OX2 PE=...	[more]
O04707	9.6e-74	52.09	Gibberellin 20 oxidase 1-A OS=Triticum aestivum OX=4565 GN=GA20ox1A PE=2 SV=1	[more]
O04705	2.8e-73	52.09	Gibberellin 20 oxidase 1-D OS=Triticum aestivum OX=4565 GN=GA20ox1D PE=1 SV=1	[more]

BLAST of HG10016919 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
S0BDZ8	1.2e-138	86.76	GA 20-oxidase OS=Cucumis sativus OX=3659 GN=GA20ox2 PE=2 SV=1	[more]
A0A5A7SLS2	1.7e-137	86.46	Gibberellin 20 oxidase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc...	[more]
A0A1S3CJ08	1.7e-137	86.46	gibberellin 20 oxidase 2-like OS=Cucumis melo OX=3656 GN=LOC103500953 PE=3 SV=1	[more]
A0A6J1EG31	2.9e-126	79.93	gibberellin 20 oxidase 1-D-like OS=Cucurbita moschata OX=3662 GN=LOC111433062 PE...	[more]
A0A6J1ITP8	3.8e-126	78.87	gibberellin 20 oxidase 1-D-like OS=Cucurbita maxima OX=3661 GN=LOC111478538 PE=3...	[more]

BLAST of HG10016919 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25420.1	4.9e-73	50.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51810.1	4.1e-72	48.38	gibberellin 20 oxidase 2	[more]
AT5G07200.1	1.5e-69	50.00	gibberellin 20-oxidase 3	[more]
AT1G60980.1	9.8e-66	47.69	gibberellin 20-oxidase 4	[more]
AT1G44090.1	2.5e-61	42.86	gibberellin 20-oxidase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 233..254 score: 36.36 coord: 69..86 score: 45.45
None	No IPR available	PANTHER	PTHR47990:SF110	GIBBERELLIN 20 OXIDASE 1-B-LIKE	coord: 15..278
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 15..278
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 12..278
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 211..278 e-value: 1.2E-17 score: 64.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 7..286 e-value: 2.3E-64 score: 220.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 47..149 e-value: 1.4E-21 score: 77.4
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 209..319 score: 9.281432

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10016919.1	HG10016919.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009908	flower development
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0009416	response to light stimulus
biological_process	GO:0009826	unidimensional cell growth
molecular_function	GO:0045544	gibberellin 20-oxidase activity
molecular_function	GO:0046872	metal ion binding