HG10016810 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016810
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionalkane hydroxylase MAH1-like
LocationChr03: 8287824 .. 8289362 (-)
RNA-Seq ExpressionHG10016810
SyntenyHG10016810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCCACAGATTTTTCAGCCATCCTTTTGGCCTTGATCATATCATTTCTTGTCATCATTTTCTCTCGAATTTGGAAGTGGAATTCCAATGGTGTGGTTATTCCTTGGAATTGGCCAATCTTAGGTATGATGCCCCATGCTGTTGTTCATGTCCATCGAATTCATGATCGTATAGCTGAATTGTTAAGTGAATCTGGTCCCAGTTTCTTCTTCAAAGGTGTTTGGTTTTCAAGTATGGACTTCTTTCTCACAGCTGACCCTTCAAATATCAACCATATTTTGAGTGTCCATTTCGAACGATATCCAAAAGGCCTTGATTTTAAGTACATTTTTGACATTTTGGGAGATGGGATTTTCAACTCTGATTCATATGTTTGGAAGGAACAACGCAAAATTGCTCATTCCTTGGTCAGCCATGACAGTTTTCTTCAATTCTTGGAGAAAATTACACTTAAGAAAGTGAAGGAAGGGTTGATTCCGGTGCTTGAAAGTGTTTGTGAAAATGGGTCGGTGTTGGATTTGCAAGATGTGTTTCAAAGATTCTCTTTTGATTCAACTTGTATGTTCGTCACTGGCTTTGACTTCCATAGTTTATCTCTTCAATTCCCTCAAGTTCCTTTCTCAAAAGCCGTGGATGAAGTTGAGGAAGTGATTATTCTTCGCCATATTATTCCTAAAATGTTGTGGGAATTTCAGAAGAAATTTCAGATTGGTCCAGCCAGGAGGATGAAAGAAGCTTGGAATGTCATAGATCAAACCATTGCAAAATTGATAGCCTCAAAAAGAGAGAGCTTGAAGAAGAAGAAGAAAGAAGAAGAAGAAGGAGTTGATTTGATCACATCATATATGAAAAACGATAAAGAAAATGATGATGATAAGGTTTTGAGAGACACTCTTTTGAATTTTATGGTTGCAGGTAGGGACACTTTAAGTTCAGCCTTCTCTTGGTTTTTCTTTTGTCTTTCAGAAAATCCAATCGTCGTAGAGAAAATCAGAGAAGAGCTCGAAACAACGATTCCACTGAATGAAGCTCGAGATCAATGGCGGATATTTTCGATTGAAGAGATAGATAAGTTAGTTTACTTCCATGGAGCTTTGTGTGAAACTCTTAGATTATATCCTCCAGTCCCAGCCAATCACAAGGTGGCCGTACAACATGATATCCTCCCAAGTGGTCATCACATAAATCCCAATACAAAGATATTGTTGCCTATATATGCATTAGGAAGAATGAGTGAAGTGTGGGGGAGAGATTGCATGGAGTACAAGCCAGAGAGATGGATTTCAGAAAATGGAAAGATCAAACATGTGCCATCTTACAAATTCTTGTCCTTCAATGCTGGGCCAAGAACTTGCTTGGGAAAACAAGTGGCTTTCACTGAGTTGAAAATCGTTTCAGCTGCCATTATTCATAACTATAATATTATTCAACAAACAGGACATCAGGTTATTCCAAATACATCCGTTATTCTTCATATGAAGCATGGATTCAAGGTCAAAGTTACAAAGAGATGGACTTTAAAGAAGTTAAATTAG

mRNA sequence

ATGGCTTCCACAGATTTTTCAGCCATCCTTTTGGCCTTGATCATATCATTTCTTGTCATCATTTTCTCTCGAATTTGGAAGTGGAATTCCAATGGTGTGGTTATTCCTTGGAATTGGCCAATCTTAGGTATGATGCCCCATGCTGTTGTTCATGTCCATCGAATTCATGATCGTATAGCTGAATTGTTAAGTGAATCTGGTCCCAGTTTCTTCTTCAAAGGTGTTTGGTTTTCAAGTATGGACTTCTTTCTCACAGCTGACCCTTCAAATATCAACCATATTTTGAGTGTCCATTTCGAACGATATCCAAAAGGCCTTGATTTTAAGTACATTTTTGACATTTTGGGAGATGGGATTTTCAACTCTGATTCATATGTTTGGAAGGAACAACGCAAAATTGCTCATTCCTTGGTCAGCCATGACAGTTTTCTTCAATTCTTGGAGAAAATTACACTTAAGAAAGTGAAGGAAGGGTTGATTCCGGTGCTTGAAAGTGTTTGTGAAAATGGGTCGGTGTTGGATTTGCAAGATGTGTTTCAAAGATTCTCTTTTGATTCAACTTGTATGTTCGTCACTGGCTTTGACTTCCATAGTTTATCTCTTCAATTCCCTCAAGTTCCTTTCTCAAAAGCCGTGGATGAAGTTGAGGAAGTGATTATTCTTCGCCATATTATTCCTAAAATGTTGTGGGAATTTCAGAAGAAATTTCAGATTGGTCCAGCCAGGAGGATGAAAGAAGCTTGGAATGTCATAGATCAAACCATTGCAAAATTGATAGCCTCAAAAAGAGAGAGCTTGAAGAAGAAGAAGAAAGAAGAAGAAGAAGGAGTTGATTTGATCACATCATATATGAAAAACGATAAAGAAAATGATGATGATAAGGTTTTGAGAGACACTCTTTTGAATTTTATGGTTGCAGAAAATCCAATCGTCGTAGAGAAAATCAGAGAAGAGCTCGAAACAACGATTCCACTGAATGAAGCTCGAGATCAATGGCGGATATTTTCGATTGAAGAGATAGATAAGTTAGTTTACTTCCATGGAGCTTTGTGTGAAACTCTTAGATTATATCCTCCAGTCCCAGCCAATCACAAGGTGGCCGTACAACATGATATCCTCCCAAGTGGTCATCACATAAATCCCAATACAAAGATATTGTTGCCTATATATGCATTAGGAAGAATGAGTGAAGTGTGGGGGAGAGATTGCATGGAGTACAAGCCAGAGAGATGGATTTCAGAAAATGGAAAGATCAAACATGTGCCATCTTACAAATTCTTGTCCTTCAATGCTGGGCCAAGAACTTGCTTGGGAAAACAAGTGGCTTTCACTGAGTTGAAAATCGTTTCAGCTGCCATTATTCATAACTATAATATTATTCAACAAACAGGACATCAGGTTATTCCAAATACATCCGTTATTCTTCATATGAAGCATGGATTCAAGGTCAAAGTTACAAAGAGATGGACTTTAAAGAAGTTAAATTAG

Coding sequence (CDS)

ATGGCTTCCACAGATTTTTCAGCCATCCTTTTGGCCTTGATCATATCATTTCTTGTCATCATTTTCTCTCGAATTTGGAAGTGGAATTCCAATGGTGTGGTTATTCCTTGGAATTGGCCAATCTTAGGTATGATGCCCCATGCTGTTGTTCATGTCCATCGAATTCATGATCGTATAGCTGAATTGTTAAGTGAATCTGGTCCCAGTTTCTTCTTCAAAGGTGTTTGGTTTTCAAGTATGGACTTCTTTCTCACAGCTGACCCTTCAAATATCAACCATATTTTGAGTGTCCATTTCGAACGATATCCAAAAGGCCTTGATTTTAAGTACATTTTTGACATTTTGGGAGATGGGATTTTCAACTCTGATTCATATGTTTGGAAGGAACAACGCAAAATTGCTCATTCCTTGGTCAGCCATGACAGTTTTCTTCAATTCTTGGAGAAAATTACACTTAAGAAAGTGAAGGAAGGGTTGATTCCGGTGCTTGAAAGTGTTTGTGAAAATGGGTCGGTGTTGGATTTGCAAGATGTGTTTCAAAGATTCTCTTTTGATTCAACTTGTATGTTCGTCACTGGCTTTGACTTCCATAGTTTATCTCTTCAATTCCCTCAAGTTCCTTTCTCAAAAGCCGTGGATGAAGTTGAGGAAGTGATTATTCTTCGCCATATTATTCCTAAAATGTTGTGGGAATTTCAGAAGAAATTTCAGATTGGTCCAGCCAGGAGGATGAAAGAAGCTTGGAATGTCATAGATCAAACCATTGCAAAATTGATAGCCTCAAAAAGAGAGAGCTTGAAGAAGAAGAAGAAAGAAGAAGAAGAAGGAGTTGATTTGATCACATCATATATGAAAAACGATAAAGAAAATGATGATGATAAGGTTTTGAGAGACACTCTTTTGAATTTTATGGTTGCAGAAAATCCAATCGTCGTAGAGAAAATCAGAGAAGAGCTCGAAACAACGATTCCACTGAATGAAGCTCGAGATCAATGGCGGATATTTTCGATTGAAGAGATAGATAAGTTAGTTTACTTCCATGGAGCTTTGTGTGAAACTCTTAGATTATATCCTCCAGTCCCAGCCAATCACAAGGTGGCCGTACAACATGATATCCTCCCAAGTGGTCATCACATAAATCCCAATACAAAGATATTGTTGCCTATATATGCATTAGGAAGAATGAGTGAAGTGTGGGGGAGAGATTGCATGGAGTACAAGCCAGAGAGATGGATTTCAGAAAATGGAAAGATCAAACATGTGCCATCTTACAAATTCTTGTCCTTCAATGCTGGGCCAAGAACTTGCTTGGGAAAACAAGTGGCTTTCACTGAGTTGAAAATCGTTTCAGCTGCCATTATTCATAACTATAATATTATTCAACAAACAGGACATCAGGTTATTCCAAATACATCCGTTATTCTTCATATGAAGCATGGATTCAAGGTCAAAGTTACAAAGAGATGGACTTTAAAGAAGTTAAATTAG

Protein sequence

MASTDFSAILLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRIAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGIFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVFQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIGPARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEEEGVDLITSYMKNDKENDDDKVLRDTLLNFMVAENPIVVEKIREELETTIPLNEARDQWRIFSIEEIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVWGRDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWTLKKLN
Homology
BLAST of HG10016810 vs. NCBI nr
Match: XP_004142037.3 (alkane hydroxylase MAH1 [Cucumis sativus])

HSP 1 Score: 778.1 bits (2008), Expect = 4.6e-221
Identity = 382/486 (78.60%), Postives = 425/486 (87.45%), Query Frame = 0

Query: 1   MASTDFSAIL-LALIISFLVIIFSRIWKWNSNGV-VIPWNWPILGMMPHAVVHVHRIHDR 60
           MAS D+SAI+ L  IISFL IIFSRIW+WNSNG+  +PWNWPILGM+PH + H HR+HDR
Sbjct: 1   MASIDYSAIIPLTFIISFLFIIFSRIWRWNSNGMETLPWNWPILGMIPHLIAHHHRVHDR 60

Query: 61  IAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDG 120
           IAE+L ES  +FFFKGVWFSS DF LTADPSN+NHILSVHFERYPKG DF YIFDILGDG
Sbjct: 61  IAEVLIESSQNFFFKGVWFSSTDFLLTADPSNVNHILSVHFERYPKGPDFNYIFDILGDG 120

Query: 121 IFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDV 180
           IFNSDS VWK QRK A SLV H+SF +FLEKITLKKVKEGL+PVL+S CENGSVLDLQD+
Sbjct: 121 IFNSDSDVWKNQRKTALSLVGHESFHKFLEKITLKKVKEGLVPVLQSACENGSVLDLQDL 180

Query: 181 FQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQI 240
           FQRFSFDSTC+FVTGFDFHSLSL+FPQVPFS+AVDE EEVI++RH+ PKMLW+F++KFQI
Sbjct: 181 FQRFSFDSTCLFVTGFDFHSLSLEFPQVPFSRAVDEAEEVILIRHVFPKMLWDFEEKFQI 240

Query: 241 GPARRMKEAWNVIDQTIAKLIASKRESLKKKKKE-EEEGVDLITSYMKNDKENDDDKVLR 300
           G A++MK+AW +IDQ IA+LIASKRESLKK  KE E+EG DLITSYMK+ KEN DDKVLR
Sbjct: 241 GQAKKMKQAWKIIDQVIAELIASKRESLKKNLKEKEDEGADLITSYMKDYKEN-DDKVLR 300

Query: 301 DTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIEEI 360
           DT+LNFMVA                 +NPIVVEKIREEL TT+P NEA DQWRIFSIEE+
Sbjct: 301 DTVLNFMVAGRDTLSAALSWFFFCLSKNPIVVEKIREELRTTVPTNEACDQWRIFSIEEV 360

Query: 361 DKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVWG 420
           DKLVYFHG+LCE+LRLYPPVP NHKVAVQ DILPSGHHI P TKILL IYALGRMS+VWG
Sbjct: 361 DKLVYFHGSLCESLRLYPPVPVNHKVAVQPDILPSGHHIKPKTKILLSIYALGRMSDVWG 420

Query: 421 RDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNII 467
           +DC+E+KPERWISENGKIKH PSYKFLSFNAGPRTCLGKQVAFTELKIV+AAIIHNYNII
Sbjct: 421 KDCLEFKPERWISENGKIKHFPSYKFLSFNAGPRTCLGKQVAFTELKIVAAAIIHNYNII 480

BLAST of HG10016810 vs. NCBI nr
Match: XP_038882620.1 (alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida])

HSP 1 Score: 769.2 bits (1985), Expect = 2.1e-218
Identity = 375/511 (73.39%), Postives = 429/511 (83.95%), Query Frame = 0

Query: 1   MASTDFSAILLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRIA 60
           MAS DFSAIL AL I FL IIFSRI +WN +GVV PWNWPI+GM P  V H++R HDR+ 
Sbjct: 1   MASIDFSAILFALFIPFLFIIFSRISRWNFHGVV-PWNWPIVGMTPTVVAHINRAHDRVT 60

Query: 61  ELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGIF 120
           E+L  +G +FFFKGVWFS MDF  T DPSNI+HILS +FERYPKG DFKYIF++LGDGIF
Sbjct: 61  EVLQAAGSTFFFKGVWFSGMDFLFTVDPSNIHHILSSNFERYPKGPDFKYIFEVLGDGIF 120

Query: 121 NSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVFQ 180
           NSDS VWK QRK AHSLV  ++FLQFLEKITLKKVKEGL+P+LESVCENGSVLDLQD+FQ
Sbjct: 121 NSDSDVWKNQRKTAHSLVHDENFLQFLEKITLKKVKEGLVPILESVCENGSVLDLQDLFQ 180

Query: 181 RFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIGP 240
           RFSFDSTCM VTGFD +SLSLQ P+VPFSKA+D+VEEVI LRH+ PK +WEF KK QIG 
Sbjct: 181 RFSFDSTCMLVTGFDLNSLSLQLPEVPFSKAMDDVEEVIFLRHVFPKKIWEFHKKLQIGQ 240

Query: 241 ARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEEE----GVDLITSYMKNDKENDDDKVL 300
            +R+K+AW +ID+TIAKLIASKR+S+K + KEE +    GVDLITSY+ N K  +DD+ L
Sbjct: 241 PKRLKQAWEIIDETIAKLIASKRKSMKNQMKEEADEQGIGVDLITSYITNTK--NDDEFL 300

Query: 301 RDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIEE 360
           RDT+LNFM+A                  NPIVV  IREEL+TTIP NE RDQ RIFSIEE
Sbjct: 301 RDTVLNFMIAGRDTLSSALSWFFFCLSNNPIVVANIREELKTTIPTNEVRDQLRIFSIEE 360

Query: 361 IDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVW 420
           +DKLVYFHG+LCE LRLYPPVP  HKV+VQHDILP+GHHINP TKIL+ +YALGRMS+VW
Sbjct: 361 VDKLVYFHGSLCEALRLYPPVPLQHKVSVQHDILPTGHHINPKTKILISLYALGRMSDVW 420

Query: 421 GRDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNI 480
           G+D ME+KPERWISENG+IKHVPSYKFL+FNAGPRTCLGKQVAFTELKIV+AAIIHNYNI
Sbjct: 421 GKDSMEFKPERWISENGRIKHVPSYKFLAFNAGPRTCLGKQVAFTELKIVAAAIIHNYNI 480

Query: 481 IQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           IQQ GH+V+PN S+ILHMKHGFKVKVTKRWT
Sbjct: 481 IQQAGHKVVPNASIILHMKHGFKVKVTKRWT 508

BLAST of HG10016810 vs. NCBI nr
Match: XP_011657793.1 (alkane hydroxylase MAH1 [Cucumis sativus] >KGN48432.1 hypothetical protein Csa_004344 [Cucumis sativus])

HSP 1 Score: 749.6 bits (1934), Expect = 1.7e-212
Identity = 365/513 (71.15%), Postives = 424/513 (82.65%), Query Frame = 0

Query: 1   MASTDFSAILLALIISFLV-IIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           MAS DFS+IL A II FLV II SRIW+W+ +G +IPWNWPI+GM P  + H+HR HDR+
Sbjct: 1   MASIDFSSILFAFIIPFLVFIILSRIWRWDFHG-IIPWNWPIVGMTPTVIAHIHRPHDRV 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E+L ++  +FFFKG+WFS MDF  T DPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVLQQTAFTFFFKGIWFSGMDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDS VWK QRK AH LV H +FLQFLEKITL KVK GL+P+L+SVCENGSVLDLQD+F
Sbjct: 121 FNSDSDVWKNQRKTAHYLVRHHNFLQFLEKITLNKVKTGLVPILDSVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD +SLSL+FP+VPFSKA+D+ EEVI +RH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLNSLSLEFPEVPFSKAMDDAEEVIFVRHFFPKTIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEE----EGVDLITSYMKNDKENDDDKV 300
             +R+K+AW +ID+TIAKLIASKR+SLK + KEE+    EGVDLITSY+ N +  +DD+ 
Sbjct: 241 QPKRLKQAWEIIDETIAKLIASKRKSLKNEMKEEDDNGKEGVDLITSYITNIE--NDDEF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
           LRDT+LNFM+A                  +P VVEKIREEL TTIP NEA DQ RIFSIE
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCLSNHPTVVEKIREELRTTIPTNEAYDQRRIFSIE 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E+D LVYFHG LCE LRLYPPVP  HKV++QHDILPSGHHI P TKIL  +YALGRMSEV
Sbjct: 361 EVDNLVYFHGTLCEALRLYPPVPLQHKVSIQHDILPSGHHIKPKTKILFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAFTELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQ GH+V+P+ S+ILHMKHGFKVKVTKRW+
Sbjct: 481 NIIQQAGHEVVPSASIILHMKHGFKVKVTKRWS 510

BLAST of HG10016810 vs. NCBI nr
Match: XP_008440138.1 (PREDICTED: alkane hydroxylase MAH1-like [Cucumis melo] >TYK12963.1 alkane hydroxylase MAH1-like [Cucumis melo var. makuwa])

HSP 1 Score: 748.8 bits (1932), Expect = 3.0e-212
Identity = 366/513 (71.35%), Postives = 422/513 (82.26%), Query Frame = 0

Query: 1   MASTDFSAILLALIISFLV-IIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           MAS DFS+IL A II+FL+ IIFSRIW+W+ +G +IPWNWPI+GM P  + H HR HDR+
Sbjct: 1   MASIDFSSILFAFIIAFLIFIIFSRIWRWDFHG-IIPWNWPIVGMTPTVIAHFHRPHDRV 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E+L ++  +FFFKG+WFS  DF  T DPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVLQQTAFTFFFKGIWFSGTDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDS VWK QRK AH LV H+SFLQFLEKITL KVK GL+PVL+SVCENGSVLDLQD+F
Sbjct: 121 FNSDSDVWKSQRKTAHHLVRHESFLQFLEKITLNKVKTGLVPVLDSVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD HSLSL+ P+VPFSKA+D+ EEVI LRH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLHSLSLESPEVPFSKAMDDAEEVIFLRHFFPKTIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLKKKKKEE----EEGVDLITSYMKNDKENDDDKV 300
             +R+K+AW +ID+TIAKLIASKR+SLK + KEE    +EGVDLITSY+ N +   DD+ 
Sbjct: 241 QPKRLKQAWEIIDETIAKLIASKRKSLKNQMKEEGGDDKEGVDLITSYITNIEY--DDEF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
           LRDT+LNFM+A                  NP VVEKIREEL T+IP NEA DQ RIFS+E
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCVSNNPTVVEKIREELRTSIPTNEAYDQRRIFSLE 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E+D LVY HG LCE LRLYPPVP  HKV+ QHDILPSGHHI P TKIL  +YALGRMSEV
Sbjct: 361 EVDNLVYLHGTLCEALRLYPPVPLQHKVSTQHDILPSGHHIKPKTKILFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAFTELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQTGH+V+P+ S+ILHMKHGFKVKVTKRW+
Sbjct: 481 NIIQQTGHEVVPSASIILHMKHGFKVKVTKRWS 510

BLAST of HG10016810 vs. NCBI nr
Match: XP_011658477.1 (alkane hydroxylase MAH1 [Cucumis sativus])

HSP 1 Score: 733.8 bits (1893), Expect = 9.8e-208
Identity = 367/513 (71.54%), Postives = 413/513 (80.51%), Query Frame = 0

Query: 1   MASTDFS-AILLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           M S +FS AILLA II FL IIF RIW+ N +G V PWNWPI+GM P  V H+HR HDRI
Sbjct: 1   MTSINFSAAILLASIILFLFIIFCRIWRRNFHGAV-PWNWPIVGMTPSVVTHIHRSHDRI 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E++ E G +FFFKGVWFS MDF LTADPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVIQEVGSTFFFKGVWFSGMDFLLTADPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDSY WK+QRK A SLV  + FLQFLEKITL KVK G++PVL+ VCENGSVLDLQD+F
Sbjct: 121 FNSDSYAWKDQRKTARSLVHDEKFLQFLEKITLNKVKTGIVPVLDGVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD  SLSL+ P VPFSKA+D+VEEVI LRH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLQSLSLESPAVPFSKAMDDVEEVIFLRHFFPKKIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLK----KKKKEEEEGVDLITSYMKNDKENDDDKV 300
              R+K+AW +ID+TIAKLIA KR SLK    K+  E+  GVDLI SYM N+  N DDK 
Sbjct: 241 QPMRLKQAWKIIDETIAKLIALKRGSLKNQVNKEGDEQGRGVDLIASYMINN-TNKDDKF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
            RDT+LNFM+A                 +NP VV+ IREEL+TTIP NEA DQ RIFS+E
Sbjct: 301 FRDTVLNFMIAGRDTLSSALSWFFFCLSKNPTVVKMIREELKTTIPSNEACDQLRIFSME 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E++KLVYFH  LCE LRLYPPVP  HKVA QHDILPSGHHI P TKI+  +YALGRMSEV
Sbjct: 361 EVNKLVYFHATLCEALRLYPPVPFQHKVATQHDILPSGHHIKPKTKIVFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAF ELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFIELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQTGHQV+PN S+ILHMKHGFKVKVTKRWT
Sbjct: 481 NIIQQTGHQVVPNASIILHMKHGFKVKVTKRWT 511

BLAST of HG10016810 vs. ExPASy Swiss-Prot
Match: Q9FVS9 (Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.0e-110
Identity = 207/498 (41.57%), Postives = 302/498 (60.64%), Query Frame = 0

Query: 15  ISFLVIIFSRIW-KWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRIAELLSESGPSFFFK 74
           I FLV +F+  + +    G  I  NWP L M+P  +  + RI+D   E+L  +  +F+FK
Sbjct: 11  IFFLVCLFTYFFLQKKPQGQPILKNWPFLRMLPGMLHQIPRIYDWTVEVLEATNLTFYFK 70

Query: 75  GVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGIFNSDSYVWKEQRKI 134
           G W S  D   TADP NI+HILS +F  YPKG +FK IFD+LG+GI   D  +W+E RK 
Sbjct: 71  GPWLSGTDMLFTADPRNIHHILSSNFGNYPKGPEFKKIFDVLGEGILTVDFELWEEMRKS 130

Query: 135 AHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVFQRFSFDSTCMFVTG 194
            H+L  +  F++        K+KEGL+P L++  +   +++LQDVFQRF FD++ + +TG
Sbjct: 131 NHALFHNQDFIELSVSSNKSKLKEGLVPFLDNAAQKNIIIELQDVFQRFMFDTSSILMTG 190

Query: 195 FDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIGPARRMKEAWNVIDQ 254
           +D  SLS++  +V F +A D  EE I  RH  P +LW  Q    IG  R+M+ A   +++
Sbjct: 191 YDPMSLSIEMLEVEFGEAADIGEEAIYYRHFKPVILWRLQNWIGIGLERKMRTALATVNR 250

Query: 255 TIAKLIASKRESLKKKKKEEEEGVDLITSYMKNDKEN------DDDKVLRDTLLNFMVA- 314
             AK+I+S+R+    + K E    D +T YM  D         + DK +RD + + ++A 
Sbjct: 251 MFAKIISSRRKEEISRAKTEPYSKDALTYYMNVDTSKYKLLKPNKDKFIRDVIFSLVLAG 310

Query: 315 ----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIEEIDKLVYFHGAL 374
                           ++P V+ K+R E+ T             F  E+++KLVY H AL
Sbjct: 311 RDTTSSVLTWFFWLLSKHPQVMAKLRHEINTK------------FDNEDLEKLVYLHAAL 370

Query: 375 CETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVWGRDCMEYKPER 434
            E++RLYPP+P NHK   + D+LPSGH ++ N+KI++ IYALGRM  VWG D +++KPER
Sbjct: 371 SESMRLYPPLPFNHKSPAKPDVLPSGHKVDANSKIVICIYALGRMRSVWGEDALDFKPER 430

Query: 435 WISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNIIQQTGHQVIPN 489
           WIS+NG ++H PSYKF++FN+GPRTCLGK +A  ++K+V+  II NY+     GH+V P 
Sbjct: 431 WISDNGGLRHEPSYKFMAFNSGPRTCLGKNLALLQMKMVALEIIRNYDFKVIEGHKVEPI 490

BLAST of HG10016810 vs. ExPASy Swiss-Prot
Match: A0A140IL90 (Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T1 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 8.9e-103
Identity = 199/510 (39.02%), Postives = 300/510 (58.82%), Query Frame = 0

Query: 1   MASTDFSAILLAL--IISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDR 60
           M S  +  IL+A+   + F +++ +R    +S+   +P+NWPI GM+P A++  ++ +D 
Sbjct: 10  MFSDHYPEILIAIACFLIFSLLLSAR----SSSEDSLPYNWPIFGMLP-AIISNNQFNDF 69

Query: 61  IAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDG 120
               L + G +F FKG W   MD+  T DPSNINH+ + +FE YPKG +   +FDI G+ 
Sbjct: 70  TTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNN 129

Query: 121 IFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDV 180
           IFN+D  +W + RK+A +++   ++          K+   LIP+L+S       +DLQDV
Sbjct: 130 IFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDV 189

Query: 181 FQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQI 240
           F RF+FD++C  V   D  SL+++FP VPFSKA D+  +  + RHI P+++W+ ++ F +
Sbjct: 190 FFRFTFDTSCFSVLAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNV 249

Query: 241 GPARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEEEGVDLITSYMKNDKENDDDKVLRD 300
           G  R +  AW VID  I   IA  +   K+K   +    D ++ YM N   + DDK LRD
Sbjct: 250 GSERTLAVAWKVIDSYIYDKIAELK--AKRKLVGKINSYDAVSFYMDNFNIH-DDKFLRD 309

Query: 301 TLLNFMVA-----------------ENPIVVEKIREELETTIPLNEAR---DQWRIFSIE 360
               +++A                 ENP V  KI  EL++ +  +  R   D + +F   
Sbjct: 310 NAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKFNDGFALFDSN 369

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
            I   +Y H  LCE LR+YPPVP   K A + D+LPSGH +    KIL   YA+ RM  +
Sbjct: 370 MIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGI 429

Query: 421 WGRDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYN 480
           WG DC+E+KPERWI+ NG +KH P+YKF +F+AGPR CLGK+++FT++K+V A II+N++
Sbjct: 430 WGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFH 489

Query: 481 IIQQTGHQVIPNTSVILHMKHGFKVKVTKR 489
           +    GH V  + S+++ MKHG  V+V KR
Sbjct: 490 LQMVKGHVVEQSNSILMDMKHGLMVQVRKR 510

BLAST of HG10016810 vs. ExPASy Swiss-Prot
Match: A0A140IL92 (Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T3 PE=2 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.5e-102
Identity = 196/500 (39.20%), Postives = 294/500 (58.80%), Query Frame = 0

Query: 9   ILLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRIAELLSESGP 68
           I +A  + F +++ +R    +S+   +P+NWPI GM+P A++   + +D     L + G 
Sbjct: 20  IAIACFLIFSLLLSAR----SSSEDSLPYNWPIFGMLP-AIISNSQFNDFTTARLRKMGW 79

Query: 69  SFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGIFNSDSYVWK 128
           +F FKG W   MD+  T DPSNINH+ + +FE YPKG +   +FDI G+ IFN+D  +W 
Sbjct: 80  TFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNNIFNADGDLWH 139

Query: 129 EQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVFQRFSFDSTC 188
           + RK+A +++   ++          K+   LIP+L+S       +DLQDVF RF+FD++C
Sbjct: 140 DHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAASKRKPVDLQDVFFRFTFDTSC 199

Query: 189 MFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIGPARRMKEAW 248
             V   D  SL+++FP VPFSKA D+  +  + RHI P+++W+ ++ F +G  R +  AW
Sbjct: 200 FSVLAADPESLTMEFPAVPFSKAADQALDAALTRHITPRLIWKLKRFFNVGSERTLAVAW 259

Query: 249 NVIDQTIAKLIASKRESLKKKKKEEEEGVDLITSYMKNDKENDDDKVLRDTLLNFMVA-- 308
            VID  I   IA  +   K+K   +    D ++ YM N   + DDK LRD    +++A  
Sbjct: 260 KVIDSYIYDKIAELK--AKRKLVGKINSYDAVSFYMDNFNIH-DDKFLRDNAFTYLLAQR 319

Query: 309 ---------------ENPIVVEKIREELETTIPLNEAR---DQWRIFSIEEIDKLVYFHG 368
                          ENP V  KI  EL++ +  +  R   D + +F    I   +Y H 
Sbjct: 320 NTQSLTMAWLFYALFENPKVELKILSELKSIVDESSERRFNDGFALFDSNMIQSAIYLHA 379

Query: 369 ALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVWGRDCMEYKP 428
            LCE LR+YPPVP   K A + D+LPSGH +    KIL   YA+ RM  +WG DC+E+KP
Sbjct: 380 TLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGIWGDDCLEFKP 439

Query: 429 ERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNIIQQTGHQVI 488
           ERWI+ NG +KH P+YKF +F+AGPR CLGK+++FT++K+V A II+N+++    GH V 
Sbjct: 440 ERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFHLQMVKGHVVE 499

BLAST of HG10016810 vs. ExPASy Swiss-Prot
Match: A0A140IL91 (Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 3.4e-102
Identity = 199/510 (39.02%), Postives = 300/510 (58.82%), Query Frame = 0

Query: 1   MASTDFSAILLAL--IISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDR 60
           M S  +  IL+A+   + F +++ +R    +S+   +P+NWPI GM+P A++  ++ +D 
Sbjct: 10  MFSDHYPEILIAIACFLIFSLLLSAR----SSSEDSLPYNWPIFGMLP-AIISNNQFNDF 69

Query: 61  IAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDG 120
               L + G +F FKG W   MD+  T DPSNINH+ + +FE YPKG +   +FDI G+ 
Sbjct: 70  TTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNN 129

Query: 121 IFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDV 180
           IFN+D  +W + RK+A +++   ++          K+   LIP+L+S       +DLQDV
Sbjct: 130 IFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRKPVDLQDV 189

Query: 181 FQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQI 240
             RF+FD++C  V   D  SL+++FP VPFSKA D+  +  + RHI P+++W+ ++ F +
Sbjct: 190 LLRFTFDTSCFSVLAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNV 249

Query: 241 GPARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEEEGVDLITSYMKNDKENDDDKVLRD 300
           G  R +  AW VID  I   IA  +   K+K   +    D ++ YM N   + DDK LRD
Sbjct: 250 GSERTLAVAWKVIDSYIYDKIAELK--AKRKLVGKINSYDAVSFYMDNFNIH-DDKFLRD 309

Query: 301 TLLNFMVA-----------------ENPIVVEKIREELETTIPLNEAR---DQWRIFSIE 360
               +++A                 ENP V  KI  EL++ +  +  R   D + +F   
Sbjct: 310 NAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKFNDGFTLFDSN 369

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
            I   +Y H ALCE LR+YPPVP   K A + D+LPSGH +    KIL   YA+ RM  +
Sbjct: 370 MIQSAIYLHAALCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGI 429

Query: 421 WGRDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYN 480
           WG DC+E+KPERWI+ NG +KH P+YKF +F+AGPR CLGK+++FT++K+V A II+N++
Sbjct: 430 WGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFH 489

Query: 481 IIQQTGHQVIPNTSVILHMKHGFKVKVTKR 489
           +    GH V  + S+++ MKHG  V+V KR
Sbjct: 490 LQMVKGHVVEQSNSILMDMKHGLMVQVRKR 510

BLAST of HG10016810 vs. ExPASy Swiss-Prot
Match: A0A2H5AIZ9 (Noroxomaritidine synthase 2 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T2 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 9.9e-102
Identity = 198/510 (38.82%), Postives = 299/510 (58.63%), Query Frame = 0

Query: 1   MASTDFSAILLAL--IISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDR 60
           M S  +  IL+A+   + F +++ +R    +S+   +P+NWPI GM+P A++  ++ +D 
Sbjct: 10  MFSDHYPEILIAIACFLIFSLLLSAR----SSSKDSLPYNWPIFGMLP-AIISNNQFNDF 69

Query: 61  IAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDG 120
               L + G +F FKG W   MD+  T DPSNINH+ + +FE YPKG +   +FDI G+ 
Sbjct: 70  TTARLRKMGWTFIFKGPWLLDMDYIFTCDPSNINHMFNDNFENYPKG-ELGKVFDIFGNN 129

Query: 121 IFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDV 180
           IFN+D  +W + RK+A +++   ++          K+   LIP+L+S       +DLQDV
Sbjct: 130 IFNADGDLWHDHRKMAQTILWDGNYRTMQATFIRNKMDNALIPILDSAACKRKPVDLQDV 189

Query: 181 FQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQI 240
             RF+FD++C  V   D  SL+++FP VPFSKA D+  +  + RHI P+++W+ ++ F +
Sbjct: 190 LLRFTFDTSCFSVLAADPESLTMEFPPVPFSKAADQALDAALTRHITPRLIWKLKRFFNV 249

Query: 241 GPARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEEEGVDLITSYMKNDKENDDDKVLRD 300
           G  R +  AW VID  I   IA  +   K+K   +    D ++ YM N   + DDK LRD
Sbjct: 250 GSERTLAVAWKVIDSYIYDKIAELK--AKRKLVGKINSYDAVSFYMDNFNIH-DDKFLRD 309

Query: 301 TLLNFMVA-----------------ENPIVVEKIREELETTIPLNEAR---DQWRIFSIE 360
               +++A                 ENP V  KI  EL++ +  +  R   D + +F   
Sbjct: 310 NAFTYLLAQRNTQSLTMTWLFYALFENPKVELKILSELKSIVDESSERKFNDGFALFDSN 369

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
            I   +Y H  LCE LR+YPPVP   K A + D+LPSGH +    KIL   YA+ RM  +
Sbjct: 370 MIQSAIYLHATLCEALRIYPPVPFEIKDAHKADVLPSGHKVRAGEKILFSPYAMARMKGI 429

Query: 421 WGRDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYN 480
           WG DC+E+KPERWI+ NG +KH P+YKF +F+AGPR CLGK+++FT++K+V A II+N++
Sbjct: 430 WGDDCLEFKPERWITGNGTLKHEPAYKFFAFSAGPRICLGKELSFTQMKMVVATIIYNFH 489

Query: 481 IIQQTGHQVIPNTSVILHMKHGFKVKVTKR 489
           +    GH V  + S+++ MKHG  V+V KR
Sbjct: 490 LQMVKGHVVEQSNSILMDMKHGLMVQVRKR 510

BLAST of HG10016810 vs. ExPASy TrEMBL
Match: A0A0A0KI04 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487550 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 2.1e-235
Identity = 404/510 (79.22%), Postives = 448/510 (87.84%), Query Frame = 0

Query: 1   MASTDFSAIL-LALIISFLVIIFSRIWKWNSNGV-VIPWNWPILGMMPHAVVHVHRIHDR 60
           MAS D+SAI+ L  IISFL IIFSRIW+WNSNG+  +PWNWPILGM+PH + H HR+HDR
Sbjct: 1   MASIDYSAIIPLTFIISFLFIIFSRIWRWNSNGMETLPWNWPILGMIPHLIAHHHRVHDR 60

Query: 61  IAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDG 120
           IAE+L ES  +FFFKGVWFSS DF LTADPSN+NHILSVHFERYPKG DF YIFDILGDG
Sbjct: 61  IAEVLIESSQNFFFKGVWFSSTDFLLTADPSNVNHILSVHFERYPKGPDFNYIFDILGDG 120

Query: 121 IFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDV 180
           IFNSDS VWK QRK A SLV H+SF +FLEKITLKKVKEGL+PVL+S CENGSVLDLQD+
Sbjct: 121 IFNSDSDVWKNQRKTALSLVGHESFHKFLEKITLKKVKEGLVPVLQSACENGSVLDLQDL 180

Query: 181 FQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQI 240
           FQRFSFDSTC+FVTGFDFHSLSL+FPQVPFS+AVDE EEVI++RH+ PKMLW+F++KFQI
Sbjct: 181 FQRFSFDSTCLFVTGFDFHSLSLEFPQVPFSRAVDEAEEVILIRHVFPKMLWDFEEKFQI 240

Query: 241 GPARRMKEAWNVIDQTIAKLIASKRESLKKKKKE-EEEGVDLITSYMKNDKENDDDKVLR 300
           G A++MK+AW +IDQ IA+LIASKRESLKK  KE E+EG DLITSYMK+ KEN DDKVLR
Sbjct: 241 GQAKKMKQAWKIIDQVIAELIASKRESLKKNLKEKEDEGADLITSYMKDYKEN-DDKVLR 300

Query: 301 DTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIEEI 360
           DT+LNFMVA                 +NPIVVEKIREEL TT+P NEA DQWRIFSIEE+
Sbjct: 301 DTVLNFMVAGRDTLSAALSWFFFCLSKNPIVVEKIREELRTTVPTNEACDQWRIFSIEEV 360

Query: 361 DKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVWG 420
           DKLVYFHG+LCE+LRLYPPVP NHKVAVQ DILPSGHHI P TKILL IYALGRMS+VWG
Sbjct: 361 DKLVYFHGSLCESLRLYPPVPVNHKVAVQPDILPSGHHIKPKTKILLSIYALGRMSDVWG 420

Query: 421 RDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNII 480
           +DC+E+KPERWISENGKIKH PSYKFLSFNAGPRTCLGKQVAFTELKIV+AAIIHNYNII
Sbjct: 421 KDCLEFKPERWISENGKIKHFPSYKFLSFNAGPRTCLGKQVAFTELKIVAAAIIHNYNII 480

Query: 481 QQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           QQTGHQVIP TSV+LHMKHGFKVKVTKRWT
Sbjct: 481 QQTGHQVIPKTSVVLHMKHGFKVKVTKRWT 509

BLAST of HG10016810 vs. ExPASy TrEMBL
Match: A0A0A0KJ78 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 8.4e-213
Identity = 365/513 (71.15%), Postives = 424/513 (82.65%), Query Frame = 0

Query: 1   MASTDFSAILLALIISFLV-IIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           MAS DFS+IL A II FLV II SRIW+W+ +G +IPWNWPI+GM P  + H+HR HDR+
Sbjct: 1   MASIDFSSILFAFIIPFLVFIILSRIWRWDFHG-IIPWNWPIVGMTPTVIAHIHRPHDRV 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E+L ++  +FFFKG+WFS MDF  T DPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVLQQTAFTFFFKGIWFSGMDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDS VWK QRK AH LV H +FLQFLEKITL KVK GL+P+L+SVCENGSVLDLQD+F
Sbjct: 121 FNSDSDVWKNQRKTAHYLVRHHNFLQFLEKITLNKVKTGLVPILDSVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD +SLSL+FP+VPFSKA+D+ EEVI +RH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLNSLSLEFPEVPFSKAMDDAEEVIFVRHFFPKTIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEE----EGVDLITSYMKNDKENDDDKV 300
             +R+K+AW +ID+TIAKLIASKR+SLK + KEE+    EGVDLITSY+ N +  +DD+ 
Sbjct: 241 QPKRLKQAWEIIDETIAKLIASKRKSLKNEMKEEDDNGKEGVDLITSYITNIE--NDDEF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
           LRDT+LNFM+A                  +P VVEKIREEL TTIP NEA DQ RIFSIE
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCLSNHPTVVEKIREELRTTIPTNEAYDQRRIFSIE 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E+D LVYFHG LCE LRLYPPVP  HKV++QHDILPSGHHI P TKIL  +YALGRMSEV
Sbjct: 361 EVDNLVYFHGTLCEALRLYPPVPLQHKVSIQHDILPSGHHIKPKTKILFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAFTELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQ GH+V+P+ S+ILHMKHGFKVKVTKRW+
Sbjct: 481 NIIQQAGHEVVPSASIILHMKHGFKVKVTKRWS 510

BLAST of HG10016810 vs. ExPASy TrEMBL
Match: A0A5D3CM15 (Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005590 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.4e-212
Identity = 366/513 (71.35%), Postives = 422/513 (82.26%), Query Frame = 0

Query: 1   MASTDFSAILLALIISFLV-IIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           MAS DFS+IL A II+FL+ IIFSRIW+W+ +G +IPWNWPI+GM P  + H HR HDR+
Sbjct: 1   MASIDFSSILFAFIIAFLIFIIFSRIWRWDFHG-IIPWNWPIVGMTPTVIAHFHRPHDRV 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E+L ++  +FFFKG+WFS  DF  T DPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVLQQTAFTFFFKGIWFSGTDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDS VWK QRK AH LV H+SFLQFLEKITL KVK GL+PVL+SVCENGSVLDLQD+F
Sbjct: 121 FNSDSDVWKSQRKTAHHLVRHESFLQFLEKITLNKVKTGLVPVLDSVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD HSLSL+ P+VPFSKA+D+ EEVI LRH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLHSLSLESPEVPFSKAMDDAEEVIFLRHFFPKTIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLKKKKKEE----EEGVDLITSYMKNDKENDDDKV 300
             +R+K+AW +ID+TIAKLIASKR+SLK + KEE    +EGVDLITSY+ N +   DD+ 
Sbjct: 241 QPKRLKQAWEIIDETIAKLIASKRKSLKNQMKEEGGDDKEGVDLITSYITNIEY--DDEF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
           LRDT+LNFM+A                  NP VVEKIREEL T+IP NEA DQ RIFS+E
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCVSNNPTVVEKIREELRTSIPTNEAYDQRRIFSLE 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E+D LVY HG LCE LRLYPPVP  HKV+ QHDILPSGHHI P TKIL  +YALGRMSEV
Sbjct: 361 EVDNLVYLHGTLCEALRLYPPVPLQHKVSTQHDILPSGHHIKPKTKILFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAFTELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQTGH+V+P+ S+ILHMKHGFKVKVTKRW+
Sbjct: 481 NIIQQTGHEVVPSASIILHMKHGFKVKVTKRWS 510

BLAST of HG10016810 vs. ExPASy TrEMBL
Match: A0A1S3B0F5 (alkane hydroxylase MAH1-like OS=Cucumis melo OX=3656 GN=LOC103484695 PE=3 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.4e-212
Identity = 366/513 (71.35%), Postives = 422/513 (82.26%), Query Frame = 0

Query: 1   MASTDFSAILLALIISFLV-IIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           MAS DFS+IL A II+FL+ IIFSRIW+W+ +G +IPWNWPI+GM P  + H HR HDR+
Sbjct: 1   MASIDFSSILFAFIIAFLIFIIFSRIWRWDFHG-IIPWNWPIVGMTPTVIAHFHRPHDRV 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E+L ++  +FFFKG+WFS  DF  T DPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVLQQTAFTFFFKGIWFSGTDFLFTVDPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDS VWK QRK AH LV H+SFLQFLEKITL KVK GL+PVL+SVCENGSVLDLQD+F
Sbjct: 121 FNSDSDVWKSQRKTAHHLVRHESFLQFLEKITLNKVKTGLVPVLDSVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD HSLSL+ P+VPFSKA+D+ EEVI LRH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLHSLSLESPEVPFSKAMDDAEEVIFLRHFFPKTIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLKKKKKEE----EEGVDLITSYMKNDKENDDDKV 300
             +R+K+AW +ID+TIAKLIASKR+SLK + KEE    +EGVDLITSY+ N +   DD+ 
Sbjct: 241 QPKRLKQAWEIIDETIAKLIASKRKSLKNQMKEEGGDDKEGVDLITSYITNIEY--DDEF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
           LRDT+LNFM+A                  NP VVEKIREEL T+IP NEA DQ RIFS+E
Sbjct: 301 LRDTVLNFMIAGRDTLSSALSWFFFCVSNNPTVVEKIREELRTSIPTNEAYDQRRIFSLE 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E+D LVY HG LCE LRLYPPVP  HKV+ QHDILPSGHHI P TKIL  +YALGRMSEV
Sbjct: 361 EVDNLVYLHGTLCEALRLYPPVPLQHKVSTQHDILPSGHHIKPKTKILFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAFTELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFTELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQTGH+V+P+ S+ILHMKHGFKVKVTKRW+
Sbjct: 481 NIIQQTGHEVVPSASIILHMKHGFKVKVTKRWS 510

BLAST of HG10016810 vs. ExPASy TrEMBL
Match: A0A0A0KFY4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487540 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 4.8e-208
Identity = 367/513 (71.54%), Postives = 413/513 (80.51%), Query Frame = 0

Query: 1   MASTDFS-AILLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRI 60
           M S +FS AILLA II FL IIF RIW+ N +G V PWNWPI+GM P  V H+HR HDRI
Sbjct: 1   MTSINFSAAILLASIILFLFIIFCRIWRRNFHGAV-PWNWPIVGMTPSVVTHIHRSHDRI 60

Query: 61  AELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGI 120
            E++ E G +FFFKGVWFS MDF LTADPSNI+HILS +FERYPKG DFKYIF++LGDGI
Sbjct: 61  TEVIQEVGSTFFFKGVWFSGMDFLLTADPSNIHHILSANFERYPKGPDFKYIFEVLGDGI 120

Query: 121 FNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVF 180
           FNSDSY WK+QRK A SLV  + FLQFLEKITL KVK G++PVL+ VCENGSVLDLQD+F
Sbjct: 121 FNSDSYAWKDQRKTARSLVHDEKFLQFLEKITLNKVKTGIVPVLDGVCENGSVLDLQDLF 180

Query: 181 QRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIG 240
           QRFSFDSTCM VTGFD  SLSL+ P VPFSKA+D+VEEVI LRH  PK +WEFQKK QIG
Sbjct: 181 QRFSFDSTCMMVTGFDLQSLSLESPAVPFSKAMDDVEEVIFLRHFFPKKIWEFQKKLQIG 240

Query: 241 PARRMKEAWNVIDQTIAKLIASKRESLK----KKKKEEEEGVDLITSYMKNDKENDDDKV 300
              R+K+AW +ID+TIAKLIA KR SLK    K+  E+  GVDLI SYM N+  N DDK 
Sbjct: 241 QPMRLKQAWKIIDETIAKLIALKRGSLKNQVNKEGDEQGRGVDLIASYMINN-TNKDDKF 300

Query: 301 LRDTLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIE 360
            RDT+LNFM+A                 +NP VV+ IREEL+TTIP NEA DQ RIFS+E
Sbjct: 301 FRDTVLNFMIAGRDTLSSALSWFFFCLSKNPTVVKMIREELKTTIPSNEACDQLRIFSME 360

Query: 361 EIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEV 420
           E++KLVYFH  LCE LRLYPPVP  HKVA QHDILPSGHHI P TKI+  +YALGRMSEV
Sbjct: 361 EVNKLVYFHATLCEALRLYPPVPFQHKVATQHDILPSGHHIKPKTKIVFSLYALGRMSEV 420

Query: 421 WGRDCMEYKPERWI-SENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNY 480
           WG+DC+E+KPERWI SENGKIKHVPSYKFL+FNAGPRTCLGK VAF ELKIV+AAIIHNY
Sbjct: 421 WGKDCLEFKPERWINSENGKIKHVPSYKFLAFNAGPRTCLGKHVAFIELKIVAAAIIHNY 480

Query: 481 NIIQQTGHQVIPNTSVILHMKHGFKVKVTKRWT 491
           NIIQQTGHQV+PN S+ILHMKHGFKVKVTKRWT
Sbjct: 481 NIIQQTGHQVVPNASIILHMKHGFKVKVTKRWT 511

BLAST of HG10016810 vs. TAIR 10
Match: AT2G23180.1 (cytochrome P450, family 96, subfamily A, polypeptide 1 )

HSP 1 Score: 449.5 bits (1155), Expect = 3.5e-126
Identity = 223/482 (46.27%), Postives = 314/482 (65.15%), Query Frame = 0

Query: 38  NWPILGMMPHAVVHVHRIHDRIAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSV 97
           NWP LGM+P  +V + R++D + ELL  S  ++ FKG  F  +D  +T DP+NI+HI+S 
Sbjct: 36  NWPFLGMLPGLLVEIPRVYDFVTELLEASNLTYPFKGPCFGGLDMLITVDPANIHHIMSS 95

Query: 98  HFERYPKGLDFKYIFDILGDGIFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKE 157
           +F  YPKG +FK IFD+LGDGIFN+DS +WK+ RK A S+++H  F +F  +  + K+++
Sbjct: 96  NFANYPKGTEFKKIFDVLGDGIFNADSELWKDLRKSAQSMMTHQDFQRFTLRTIMSKLEK 155

Query: 158 GLIPVLESVCENGSVLDLQDVFQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEE 217
           GL+P+L+ V E   V+DLQDVFQRF+FD++ +  TG D   LS + PQ+ F++A+DE EE
Sbjct: 156 GLVPLLDYVAEKKQVVDLQDVFQRFTFDTSFVLATGVDPGCLSTEMPQIEFARALDEAEE 215

Query: 218 VIILRHIIPKMLWEFQKKFQIGPARRMKEAWNVIDQTIAKLIASKRESLKKKK-KEEEEG 277
            I  RH+ P+++W+ Q+    G   +MK+A +  D+  +K IASKR+ +       +   
Sbjct: 216 AIFFRHVKPEIVWKMQRFIGFGDELKMKKAHSTFDRVCSKCIASKRDEITNGVINIDSSS 275

Query: 278 VDLITSYMKNDK----------ENDDDKVLRDTLLNFMVA-----------------ENP 337
            DL+  YM  D              DDK LRD +L+FM+A                 +NP
Sbjct: 276 KDLLMCYMNVDTICHTTKYKLLNPSDDKFLRDMILSFMLAGRDTTSSALTWFFWLLSKNP 335

Query: 338 IVVEKIREELETTIPLNEARDQWRIFSIEEIDKLVYFHGALCETLRLYPPVPANHKVAVQ 397
             + KIR+E+ T   L+   + +  F+ +E++KLVY HGALCE LRLYPPVP  HK   +
Sbjct: 336 KAITKIRQEINT--QLSPRTNDFDSFNAQELNKLVYVHGALCEALRLYPPVPFQHKSPTK 395

Query: 398 HDILPSGHHINPNTKILLPIYALGRMSEVWGRDCMEYKPERWISENGKIKHVPSYKFLSF 457
            D+LPSGH ++ ++KI+  +Y+LGRM  VWG D  E+KPERWISE+G++ HVPS+KFLSF
Sbjct: 396 SDVLPSGHRVDASSKIVFCLYSLGRMKSVWGEDASEFKPERWISESGRLIHVPSFKFLSF 455

Query: 458 NAGPRTCLGKQVAFTELKIVSAAIIHNYNIIQQTGHQVIPNTSVILHMKHGFKVKVTKRW 492
           NAGPRTCLGK+VA T++K V+  II NY I    GH++ P  S+ILHMKHG KV VTKR 
Sbjct: 456 NAGPRTCLGKEVAMTQMKTVAVKIIQNYEIKVVEGHKIEPVPSIILHMKHGLKVTVTKRS 515

BLAST of HG10016810 vs. TAIR 10
Match: AT4G39480.1 (cytochrome P450, family 96, subfamily A, polypeptide 9 )

HSP 1 Score: 434.1 bits (1115), Expect = 1.5e-121
Identity = 216/480 (45.00%), Postives = 312/480 (65.00%), Query Frame = 0

Query: 38  NWPILGMMPHAVVHVHRIHDRIAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSV 97
           NWP+LGM+P  +  + R++D I E+L +   ++ F G +   +D   T DP+NI+HI+S 
Sbjct: 35  NWPLLGMLPGLLTVLPRVYDFITEVLEDGNLNYLFIGPFLGGIDMLFTVDPANIHHIMSS 94

Query: 98  HFERYPKGLDFKYIFDILGDGIFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKE 157
           +F  YPKG +FK +FD+LGDGIFN+DS +WK+ RK + S+++H  F +F    +L K+++
Sbjct: 95  NFANYPKGTEFKKLFDVLGDGIFNADSDLWKDLRKSSQSMMNHPDFQRFSLATSLSKLEK 154

Query: 158 GLIPVLESVCENGSVLDLQDVFQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEE 217
           GL+P+L+ V +   V+DLQDVFQRF+FD+T +  TG+D   LS++ P++ F++A+D+ EE
Sbjct: 155 GLVPLLDHVAKEKLVVDLQDVFQRFTFDTTFVLATGYDPGCLSVEMPEIEFARALDDAEE 214

Query: 218 VIILRHIIPKMLWEFQKKFQIGPARRMKEAWNVIDQTIAKLIASKRESLKKKKKEEEEGV 277
            I  RH  P+++W+ Q+   +G   ++K A  + D+  +K IASKR+ +  +  +     
Sbjct: 215 AIFYRHFKPEVVWKMQRLIGVGAELKLKRAHAIFDRVCSKCIASKRDEI-SQGIDSSSSK 274

Query: 278 DLITSYMKNDKEN------DDDKVLRDTLLNFMVA-----------------ENPIVVEK 337
           DL+ S +  D          DD+ LRDT+L+FM+A                  N   + K
Sbjct: 275 DLLMSSINVDTTKYKLLNPSDDRFLRDTILSFMLAGRDTTGSALTWFFWLLCNNQEAMTK 334

Query: 338 IREELETTI-PLNEARD-----QWRIFSIEEIDKLVYFHGALCETLRLYPPVPANHKVAV 397
           IR+E+ T + P N+  D         F+ +E+ KLVY HGA+CE LRLYPPVP NHK   
Sbjct: 335 IRQEINTNLFPRNKTDDGSVSYDSDSFNPQEVKKLVYLHGAVCEALRLYPPVPFNHKSPA 394

Query: 398 QHDILPSGHHINPNTKILLPIYALGRMSEVWGRDCMEYKPERWISENGKIKHVPSYKFLS 457
           + D+LPSGH +  N++IL  +Y+LGRM  VWG D ME+KPERWISE+G+  H PSYKFLS
Sbjct: 395 KPDVLPSGHKVKANSRILFCLYSLGRMKSVWGEDAMEFKPERWISESGRSVHEPSYKFLS 454

Query: 458 FNAGPRTCLGKQVAFTELKIVSAAIIHNYNIIQQTGHQVIPNTSVILHMKHGFKVKVTKR 489
           FNAGPRTCLGK+VA T++K V+  II NY+I    GH++ P  SVILHMKHG KV V+KR
Sbjct: 455 FNAGPRTCLGKEVAMTQMKTVAVKIIQNYDINVVEGHKIKPAPSVILHMKHGLKVTVSKR 513

BLAST of HG10016810 vs. TAIR 10
Match: AT2G21910.1 (cytochrome P450, family 96, subfamily A, polypeptide 5 )

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-121
Identity = 222/506 (43.87%), Postives = 314/506 (62.06%), Query Frame = 0

Query: 10  LLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRIAELLSESGPS 69
           L+ + I+ LV  F        +  + P NWP+LGM+P  +V +HRI+D +AE+L  S  +
Sbjct: 6   LVEVFIALLVFFFFHFLIHKKSHQITPRNWPVLGMLPGVLVMLHRINDYVAEILEVSNLT 65

Query: 70  FFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGIFNSDSYVWKE 129
           F FKG WFS M+  +TADPSNI H+ S +F  Y KG +FK +FD LG+GIF +DS +W++
Sbjct: 66  FAFKGPWFSGMNMLITADPSNIQHVFSSNFSNYDKGPEFKEMFDFLGNGIFTADSKLWED 125

Query: 130 QRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVFQRFSFDSTCM 189
            RK A  ++SH  F  F  +    K+K GL+PVL+   E  +V DLQDVFQR +FD T  
Sbjct: 126 MRKSALVVLSHQGFQSFSLRTITCKIKNGLVPVLDHFAEANTVFDLQDVFQRLAFDVTLT 185

Query: 190 FVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIGPARRMKEAWN 249
            VTG D  SLS++ P+  ++KA+D+ EEV++ RH+ P +LW+ Q    +G  ++MKEA  
Sbjct: 186 LVTGCDSSSLSIEMPKNEYAKAMDDAEEVVVYRHVKPVVLWKLQNWIGLGEEKKMKEANA 245

Query: 250 VIDQTIAKLIASKRESLKKKKKE---EEEGVDLITSYMKNDKEN------DDDKVLRDTL 309
             D++ AK I++KRE +         E    DL++ YM  D         +DD  L+D +
Sbjct: 246 AFDRSCAKYISAKREEIISHHSNIGGEAHAEDLLSVYMNLDISKYELLNPNDDNFLKDII 305

Query: 310 LNFMVA-----------------ENPIVVEKIREELETTIPLNEARDQWRIFSIEEIDKL 369
            +FM+A                 +NP  V KIR+E+ T +P             ++++K+
Sbjct: 306 KSFMLAGRDAIATTLTWFFWLLSKNPEAVTKIRQEINTNLP-----GSGMSLDADKLNKM 365

Query: 370 VYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGRMSEVWGRDC 429
           VY HGALCE+LRLY P+P   K  ++ D+LPSGH ++ N KIL  +YALGRM  VWG+D 
Sbjct: 366 VYLHGALCESLRLYAPIPFERKTPIKQDVLPSGHMVDKNWKILFSVYALGRMRSVWGQDA 425

Query: 430 MEYKPERWISE-NGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAIIHNYNIIQQ 489
            E+KPERWISE NG +KH PS+KF  FN+GPR CLGK ++F ++K V+  II NY+I   
Sbjct: 426 SEFKPERWISERNGGLKHEPSFKFFVFNSGPRNCLGKNLSFLQMKTVAVEIIRNYDIKVV 485

BLAST of HG10016810 vs. TAIR 10
Match: AT4G39490.1 (cytochrome P450, family 96, subfamily A, polypeptide 10 )

HSP 1 Score: 432.6 bits (1111), Expect = 4.4e-121
Identity = 223/514 (43.39%), Postives = 320/514 (62.26%), Query Frame = 0

Query: 6   FSAILLALIISFLVIIFSRIWKWNSNGVVIPWNWPILGMMPHAVVHVHRIHDRIAELLSE 65
           F ++    I  F  ++F           + P NWP  GM+P  +V +HR++D I E+L  
Sbjct: 3   FISLFEISIAFFCFLLFRHFLINKKTHRLCPTNWPFFGMIPGLLVEIHRVYDFITEILEV 62

Query: 66  SGPSFFFKGVWFSSMDFFLTADPSNINHILSVHFERYPKGLDFKYIFDILGDGIFNSDSY 125
           +  ++   G  F+++D  +T DP+NI+HI+S +F  YPKG +FK +FDILGDGIFN+DS 
Sbjct: 63  TNLTYPCTGPCFANLDMLVTVDPANIHHIMSSNFANYPKGPEFKKLFDILGDGIFNADSE 122

Query: 126 VWKEQRKIAHSLVSHDSFLQFLEKITLKKVKEGLIPVLESVCENGSVLDLQDVFQRFSFD 185
           +WK+ RK A S++ +  F +F    +LKK+++GL+P+L+ V +    +DLQD+FQRF+FD
Sbjct: 123 LWKDLRKSAQSMMMNPEFQKFSLATSLKKLEKGLVPLLDHVAKEKLAVDLQDMFQRFTFD 182

Query: 186 STCMFVTGFDFHSLSLQFPQVPFSKAVDEVEEVIILRHIIPKMLWEFQKKFQIGPARRMK 245
           +T +  TG+D   LS++ P+V F++A+D+ EE I  RH+ P++ W  Q    +G  ++M 
Sbjct: 183 TTFVLATGYDPGCLSVEMPEVEFARALDDAEEAIFFRHVKPEIFWRLQGLLGLGDEKKMT 242

Query: 246 EAWNVIDQTIAKLIASKRESLKK-KKKEEEEGVDLITSYMKNDKEN------DDDKVLRD 305
           +A + +D+  +K IA KR+ + +     +    DL+TSYM  D          D++ LRD
Sbjct: 243 KARSTLDRVCSKYIAIKRDEVSRGTNNVDSHSKDLLTSYMNLDTTKYKLLNPSDERFLRD 302

Query: 306 TLLNFMVA-----------------ENPIVVEKIREELETTIPLNEARD-------QWRI 365
           T+L FM+A                 +NP V+ KIR+E+ T +      D           
Sbjct: 303 TILTFMLAGRDTTGSGLTWFFWLLIKNPEVIAKIRQEINTALFQRSKVDDDASNNNDSDS 362

Query: 366 FSIEEIDKLVYFHGALCETLRLYPPVPANHKVAVQHDILPSGHHINPNTKILLPIYALGR 425
           FS +E+ KLVY HGA+CE+LRLYPPVP  HK   + D+LPSGH ++ N+KIL  +Y+LGR
Sbjct: 363 FSPQELKKLVYLHGAICESLRLYPPVPFQHKSPTKPDVLPSGHKVDANSKILFCLYSLGR 422

Query: 426 MSEVWGRDCMEYKPERWISENGKIKHVPSYKFLSFNAGPRTCLGKQVAFTELKIVSAAII 485
           M  VWG D +E+KPERWISE+G   H PSYKFLSFNAGPRTCLGK+VA  ++K V+  II
Sbjct: 423 MKSVWGEDALEFKPERWISESGNSVHEPSYKFLSFNAGPRTCLGKEVAMMQMKSVAVKII 482

Query: 486 HNYNIIQQTGHQVIPNTSVILHMKHGFKVKVTKR 489
            NY +    G Q+ P  SVILHMKHG KV VTKR
Sbjct: 483 QNYEMKIVEGQQIEPAPSVILHMKHGLKVTVTKR 516

BLAST of HG10016810 vs. TAIR 10
Match: AT1G65340.1 (cytochrome P450, family 96, subfamily A, polypeptide 3 )

HSP 1 Score: 417.2 bits (1071), Expect = 1.9e-116
Identity = 203/476 (42.65%), Postives = 300/476 (63.03%), Query Frame = 0

Query: 38  NWPILGMMPHAVVHVHRIHDRIAELLSESGPSFFFKGVWFSSMDFFLTADPSNINHILSV 97
           NWP LGM+P  ++ V RI+D I E+L  +  +F FKG   S MD  LT DP NI++ILS 
Sbjct: 36  NWPALGMLPGLLLQVPRIYDWITEVLEATDMTFCFKGPCLSGMDILLTVDPVNIHYILSS 95

Query: 98  HFERYPKGLDFKYIFDILGDGIFNSDSYVWKEQRKIAHSLVSHDSFLQFLEKITLKKVKE 157
           +F  YPKG++FK IF+++GD IFN DS +W++ R  +H++ S+  F  F    +++K+++
Sbjct: 96  NFANYPKGMEFKKIFEVVGDSIFNVDSGLWEDMRNSSHAIFSNQDFQMFWVSTSVRKLRQ 155

Query: 158 GLIPVLESVCENGSVLDLQDVFQRFSFDSTCMFVTGFDFHSLSLQFPQVPFSKAVDEVEE 217
           GL+P+LE+  +   ++DLQD+FQRF FD++ + +TG+D   LS++ P+V F  AVD V +
Sbjct: 156 GLVPILENAADKNILVDLQDLFQRFLFDTSLILMTGYDPKCLSVEMPKVEFGDAVDGVSD 215

Query: 218 VIILRHIIPKMLWEFQKKFQIGPARRMKEAWNVIDQTIAKLIASKRESLKK---KKKEEE 277
            +  RH+ P  LW  Q    +G  +R+K    V DQ + K+I +KRE +           
Sbjct: 216 GVFYRHVKPVFLWRLQYLIGVGVEKRLKRGLAVFDQLLEKIITAKREEINSHGTHHPSRG 275

Query: 278 EGVDLITSYMKNDK------ENDDDKVLRDTLLNFMVA-----------------ENPIV 337
           E +D++T YM  D       E  DD+ ++DT+L F++A                 +NP  
Sbjct: 276 EAIDVLTYYMTMDTTKYKYLEPSDDRFIKDTILGFLIAARDTTSSALTWFFWLMSKNPEA 335

Query: 338 VEKIREELETTIPLNEARDQWRIFSIEEIDKLVYFHGALCETLRLYPPVPANHKVAVQHD 397
           + KIR+E+   +P          F   +++KLVY HGA+CETLRLYPPVP NHK   + D
Sbjct: 336 INKIRQEVNKKMPR---------FDPADLEKLVYLHGAVCETLRLYPPVPFNHKSPAKPD 395

Query: 398 ILPSGHHINPNTKILLPIYALGRMSEVWGRDCMEYKPERWISENGKIKHVPSYKFLSFNA 457
           +LPSGH ++   KI++ +YALGRM  VWG D  +++PERWIS++G++KH PSYKFL+FNA
Sbjct: 396 VLPSGHRVDEKWKIVISMYALGRMKSVWGDDAEDFRPERWISDSGRLKHEPSYKFLAFNA 455

Query: 458 GPRTCLGKQVAFTELKIVSAAIIHNYNIIQQTGHQVIPNTSVILHMKHGFKVKVTK 488
           GPR CLGK++ F ++K V+A II NY+I    GH+  P  SV+  M+HG KV +T+
Sbjct: 456 GPRACLGKKLTFLQMKTVAAEIIRNYDIKVVEGHKTEPVPSVLFRMQHGLKVNITR 502

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_004142037.34.6e-22178.60alkane hydroxylase MAH1 [Cucumis sativus][more]
XP_038882620.12.1e-21873.39alkane hydroxylase MAH1-like isoform X1 [Benincasa hispida][more]
XP_011657793.11.7e-21271.15alkane hydroxylase MAH1 [Cucumis sativus] >KGN48432.1 hypothetical protein Csa_0... [more]
XP_008440138.13.0e-21271.35PREDICTED: alkane hydroxylase MAH1-like [Cucumis melo] >TYK12963.1 alkane hydrox... [more]
XP_011658477.19.8e-20871.54alkane hydroxylase MAH1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q9FVS92.0e-11041.57Alkane hydroxylase MAH1 OS=Arabidopsis thaliana OX=3702 GN=CYP96A15 PE=2 SV=1[more]
A0A140IL908.9e-10339.02Noroxomaritidine synthase OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222 G... [more]
A0A140IL921.5e-10239.20Noroxomaritidine synthase 3 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A140IL913.4e-10239.02Noroxomaritidine synthase 2 OS=Narcissus aff. pseudonarcissus MK-2014 OX=1540222... [more]
A0A2H5AIZ99.9e-10238.82Noroxomaritidine synthase 2 OS=Narcissus pseudonarcissus OX=39639 GN=Cyp96T2 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KI042.1e-23579.22Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487550 PE=3 SV=1[more]
A0A0A0KJ788.4e-21371.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487530 PE=3 SV=1[more]
A0A5D3CM151.4e-21271.35Alkane hydroxylase MAH1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3B0F51.4e-21271.35alkane hydroxylase MAH1-like OS=Cucumis melo OX=3656 GN=LOC103484695 PE=3 SV=1[more]
A0A0A0KFY44.8e-20871.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G487540 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G23180.13.5e-12646.27cytochrome P450, family 96, subfamily A, polypeptide 1 [more]
AT4G39480.11.5e-12145.00cytochrome P450, family 96, subfamily A, polypeptide 9 [more]
AT2G21910.13.3e-12143.87cytochrome P450, family 96, subfamily A, polypeptide 5 [more]
AT4G39490.14.4e-12143.39cytochrome P450, family 96, subfamily A, polypeptide 10 [more]
AT1G65340.11.9e-11642.65cytochrome P450, family 96, subfamily A, polypeptide 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 255..275
NoneNo IPR availablePANTHERPTHR24296:SF1CYTOCHROME P450, FAMILY 96, SUBFAMILY A, POLYPEPTIDE 10coord: 20..487
NoneNo IPR availablePANTHERPTHR24296CYTOCHROME P450coord: 20..487
IPR001128Cytochrome P450PRINTSPR00385P450coord: 2..19
score: 22.2
coord: 427..436
score: 50.56
coord: 348..359
score: 38.96
coord: 436..447
score: 42.12
IPR001128Cytochrome P450PFAMPF00067p450coord: 305..484
e-value: 1.1E-33
score: 116.7
coord: 38..306
e-value: 7.8E-18
score: 64.5
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 347..365
score: 24.34
coord: 426..436
score: 47.07
coord: 436..459
score: 26.64
coord: 298..324
score: 21.07
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 18..492
e-value: 8.4E-84
score: 283.8
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 25..488
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 429..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016810.1HG10016810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen