HG10016797 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10016797
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	E3 ubiquitin-protein ligase RNF185
Location	Chr03: 8166033 .. 8166719 (-)
RNA-Seq Expression	HG10016797
Synteny	HG10016797

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGAATGAGTTTGGTGAATCAACAAGTGGTCCACATCAAAACCCATCTTGGTCCACTAATAGTGACACTACTAACAATGATACTGGTGATTTTGAATGCAATATTTGCTTTGAGTTAGCTCAAGATCCAATTATCACTCTTTGTGGTCATCTCTTTTGCTGGCCTTGTTTATATAGATGGCTGCATCATCACTCACAATGTCAAGAATGTCCTGTTTGTAAAGCTCTTATTCAGGAGGAAAAATTGGTTCCCCTTTATGGTAGGGGTAAAATTCCATCTGATCCAAGATTAAATAGATATCCTGGTATTGATATTCCAAGTCGTCCTGCTGGGCAAAGACCTCAAACAGCACCACCCCCTGTTCCTAATGACTTTGCAAACTATGGATTTGGATTCACTGGTGGCTTTATGCCAATGGCATCAGCAAGGATTGGCAACTTCACTTTAGCTACTGCTTTTGGTGGTTTGATCCCATCTTTATTCAACATCCAGTTTCATGGATTTCCTGATGCTACTGTTTATGGAACTACTTCTGGTTACCCTTATGCATTCAACACCTTCCATGGAGGTCATGGTCACCATTTTCCGCAACCGACGACTCGAGGTCAGCATGCTGATAACGTACTGAAAAACCTCCTTTTACTCGTTGGTGTCTTTGTAATTCTTGCCTTGCTCTGGTGGTAA

mRNA sequence

ATGGCGAATGAGTTTGGTGAATCAACAAGTGGTCCACATCAAAACCCATCTTGGTCCACTAATAGTGACACTACTAACAATGATACTGGTGATTTTGAATGCAATATTTGCTTTGAGTTAGCTCAAGATCCAATTATCACTCTTTGTGGTCATCTCTTTTGCTGGCCTTGTTTATATAGATGGCTGCATCATCACTCACAATGTCAAGAATGTCCTGTTTGTAAAGCTCTTATTCAGGAGGAAAAATTGGTTCCCCTTTATGGTAGGGGTAAAATTCCATCTGATCCAAGATTAAATAGATATCCTGGTATTGATATTCCAAGTCGTCCTGCTGGGCAAAGACCTCAAACAGCACCACCCCCTGTTCCTAATGACTTTGCAAACTATGGATTTGGATTCACTGGTGGCTTTATGCCAATGGCATCAGCAAGGATTGGCAACTTCACTTTAGCTACTGCTTTTGGTGGTTTGATCCCATCTTTATTCAACATCCAGTTTCATGGATTTCCTGATGCTACTGTTTATGGAACTACTTCTGGTTACCCTTATGCATTCAACACCTTCCATGGAGGTCATGGTCACCATTTTCCGCAACCGACGACTCGAGGTCAGCATGCTGATAACGTACTGAAAAACCTCCTTTTACTCGTTGGTGTCTTTGTAATTCTTGCCTTGCTCTGGTGGTAA

Coding sequence (CDS)

ATGGCGAATGAGTTTGGTGAATCAACAAGTGGTCCACATCAAAACCCATCTTGGTCCACTAATAGTGACACTACTAACAATGATACTGGTGATTTTGAATGCAATATTTGCTTTGAGTTAGCTCAAGATCCAATTATCACTCTTTGTGGTCATCTCTTTTGCTGGCCTTGTTTATATAGATGGCTGCATCATCACTCACAATGTCAAGAATGTCCTGTTTGTAAAGCTCTTATTCAGGAGGAAAAATTGGTTCCCCTTTATGGTAGGGGTAAAATTCCATCTGATCCAAGATTAAATAGATATCCTGGTATTGATATTCCAAGTCGTCCTGCTGGGCAAAGACCTCAAACAGCACCACCCCCTGTTCCTAATGACTTTGCAAACTATGGATTTGGATTCACTGGTGGCTTTATGCCAATGGCATCAGCAAGGATTGGCAACTTCACTTTAGCTACTGCTTTTGGTGGTTTGATCCCATCTTTATTCAACATCCAGTTTCATGGATTTCCTGATGCTACTGTTTATGGAACTACTTCTGGTTACCCTTATGCATTCAACACCTTCCATGGAGGTCATGGTCACCATTTTCCGCAACCGACGACTCGAGGTCAGCATGCTGATAACGTACTGAAAAACCTCCTTTTACTCGTTGGTGTCTTTGTAATTCTTGCCTTGCTCTGGTGGTAA

Protein sequence

MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPPPVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSGYPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW

Homology

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BLAST of HG10016797 vs. NCBI nr
Match: XP_038881107.1 (E3 ubiquitin-protein ligase RNF185-like [Benincasa hispida])

HSP 1 Score: 479.6 bits (1233), Expect = 1.5e-131
Identity = 222/228 (97.37%), Postives = 225/228 (98.68%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PHQN SWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNSSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVPNDF NYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFPNYGFGFTGGFMPMATARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP+TRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of HG10016797 vs. NCBI nr
Match: XP_008440158.1 (PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo] >XP_008440160.1 PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo])

HSP 1 Score: 478.4 bits (1230), Expect = 3.4e-131
Identity = 223/228 (97.81%), Postives = 226/228 (99.12%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PH+NPS STNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP+TRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of HG10016797 vs. NCBI nr
Match: TYK12950.1 (E3 ubiquitin-protein ligase RNF185 [Cucumis melo var. makuwa])

HSP 1 Score: 478.0 bits (1229), Expect = 4.5e-131
Identity = 222/228 (97.37%), Postives = 226/228 (99.12%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PH+NPS STNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGKIPSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP+TRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

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BLAST of HG10016797 vs. NCBI nr
Match: XP_022949606.1 (E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022978947.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita maxima] >XP_023543222.1 E3 ubiquitin-protein ligase RNF185-like [Cucurbita pepo subsp. pepo] >KAG7034387.1 RING finger protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 469.5 bits (1207), Expect = 1.6e-128
Identity = 216/228 (94.74%), Postives = 221/228 (96.93%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PHQNPSWS+NSD TNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGK PSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P  NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP +RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

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BLAST of HG10016797 vs. NCBI nr
Match: KAG6604228.1 (E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 469.5 bits (1207), Expect = 1.6e-128
Identity = 216/228 (94.74%), Postives = 221/228 (96.93%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PHQNPSWS+NSD TNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 59  MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 118

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGK PSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 119 WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 178

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P  NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 179 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 238

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP +RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 239 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 286

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BLAST of HG10016797 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 1.7e-27
Identity = 82/245 (33.47%), Postives = 116/245 (47.35%), Query Frame = 0


Query: 9   TSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHHHSQC 68
           T  P + P+ S+N D    ++  FECNIC + A+D +++LCGHLFCWPCL +WL      
Sbjct: 5   TKAPSEEPTSSSNKD----ESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWLDTRPNN 64

Query: 69  QECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPPPVPNDFAN 128
           Q CPVCK+ I   K+VP+YGRG   SDPR        +P RP GQR +    P P  FA 
Sbjct: 65  QVCPVCKSAIDGNKVVPIYGRGGDSSDPRKK------VPPRPKGQRSE----PPPQSFAG 124

Query: 129 YGFGFTGGFMPMASARIG-NFTLATAFGGLIPSLFNIQF---------------HGFPDA 188
           + +G  GG M      +  +F + T   GL P +F + F               +G   A
Sbjct: 125 FNWGGDGGMMGGGGPNVHFSFGIGTV-NGLFPLMFMLPFIQGIFPLSFVASFFGNGNQGA 184

Query: 189 TVYGTTSG---------YPYAFNTFHGGHGH-HFPQPTTRGQHADNVLKNLLLLVGVFVI 228
              G  +G         + +     HG  GH     P +R    +  L N+   +G F++
Sbjct: 185 AAAGGGNGGGNDGNDGTHAHTHGHTHGPRGHGESAAPGSRMAQEEEYLSNIFKYIGFFML 234

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BLAST of HG10016797 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.0e-25
Identity = 77/177 (43.50%), Postives = 93/177 (52.54%), Query Frame = 0


Query: 2   ANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYRW 61
           A+    ST G   + S +   D TN D   FECNIC + A+D +I+LCGHLFCWPCL++W
Sbjct: 10  ASTKSSSTGGTSGSSSSNGAGDNTNQD-NTFECNICLDTAKDAVISLCGHLFCWPCLHQW 69

Query: 62  LHHHSQCQECPVCKALIQEEKLVPLYGRGKI-PSDPRLNRYPGIDIPSRPAGQRPQTAPP 121
           L      Q CPVCKA I  +K++PLYGRG     DPR         P RP GQRP+   P
Sbjct: 70  LETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQRPE---P 129

Query: 122 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGT 178
                F  +GFG  GGF    S  IG F       G+  + FNI   G P   V GT
Sbjct: 130 ENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAVPGT 167

[Close]

BLAST of HG10016797 vs. ExPASy Swiss-Prot
Match: Q5RFK9 (E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.7e-24
Identity = 76/183 (41.53%), Postives = 97/183 (53.01%), Query Frame = 0


Query: 1   MANEFGESTSGPHQN----PSWSTN-SDTTNNDTGDFECNICFELAQDPIITLCGHLFCW 60
           MA++   ++S P  +    PS S+N +  +      FECNIC + A+D +I+LCGHLFCW
Sbjct: 1   MASKGPSASSSPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCW 60

Query: 61  PCLYRWLHHHSQCQECPVCKALIQEEKLVPLYGRGKI-PSDPRLNRYPGIDIPSRPAGQR 120
           PCL++WL      Q CPVCKA I  +K++PLYGRG     DPR         P RP GQR
Sbjct: 61  PCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQR 120

Query: 121 PQTAPPPVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATV 178
           P+   P     F  +GFG  GGF    S  IG F       G+  + FNI   G P   V
Sbjct: 121 PE---PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAV 165

[Close]

BLAST of HG10016797 vs. ExPASy Swiss-Prot
Match: Q96GF1 (E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 3.8e-24
Identity = 75/183 (40.98%), Postives = 97/183 (53.01%), Query Frame = 0


Query: 1   MANEFGESTSGPHQN----PSWSTN-SDTTNNDTGDFECNICFELAQDPIITLCGHLFCW 60
           MA++   +++ P  +    PS S+N +  +      FECNIC + A+D +I+LCGHLFCW
Sbjct: 1   MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCW 60

Query: 61  PCLYRWLHHHSQCQECPVCKALIQEEKLVPLYGRGKI-PSDPRLNRYPGIDIPSRPAGQR 120
           PCL++WL      Q CPVCKA I  +K++PLYGRG     DPR         P RP GQR
Sbjct: 61  PCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQR 120

Query: 121 PQTAPPPVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATV 178
           P+   P     F  +GFG  GGF    S  IG F       G+  + FNI   G P   V
Sbjct: 121 PE---PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAV 165

[Close]

BLAST of HG10016797 vs. ExPASy Swiss-Prot
Match: Q91YT2 (E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.0e-24
Identity = 76/179 (42.46%), Postives = 93/179 (51.96%), Query Frame = 0


Query: 6   GESTSGPHQN-----PSWSTN-SDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLY 65
           G S S   +N     PS S+N +  +      FECNIC + A+D +I+LCGHLFCWPCL+
Sbjct: 5   GPSASASTENSNAGGPSGSSNGTGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLH 64

Query: 66  RWLHHHSQCQECPVCKALIQEEKLVPLYGRGKI-PSDPRLNRYPGIDIPSRPAGQRPQTA 125
           +WL      Q CPVCKA I  +K++PLYGRG     DPR         P RP GQRP+  
Sbjct: 65  QWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREK------TPPRPQGQRPE-- 124

Query: 126 PPPVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGT 178
            P     F  +GFG  GGF    S  IG F       G+  + FNI   G P   V GT
Sbjct: 125 -PENRGGFQGFGFG-DGGF--QMSFGIGAFPF-----GIFATAFNIN-DGRPPPAVPGT 165

[Close]

BLAST of HG10016797 vs. ExPASy TrEMBL
Match: A0A1S3B021 (E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo OX=3656 GN=LOC103484707 PE=4 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 1.7e-131
Identity = 223/228 (97.81%), Postives = 226/228 (99.12%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PH+NPS STNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP+TRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

[Close]

BLAST of HG10016797 vs. ExPASy TrEMBL
Match: A0A5D3CNC7 (E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G005460 PE=4 SV=1)

HSP 1 Score: 478.0 bits (1229), Expect = 2.2e-131
Identity = 222/228 (97.37%), Postives = 226/228 (99.12%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PH+NPS STNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSMPHRNPSCSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGKIPSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKIPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP+TRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 228

[Close]

BLAST of HG10016797 vs. ExPASy TrEMBL
Match: A0A6J1IMG5 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111478748 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 7.7e-129
Identity = 216/228 (94.74%), Postives = 221/228 (96.93%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PHQNPSWS+NSD TNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGK PSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P  NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP +RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

[Close]

BLAST of HG10016797 vs. ExPASy TrEMBL
Match: A0A6J1GCG3 (E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC111452948 PE=4 SV=1)

HSP 1 Score: 469.5 bits (1207), Expect = 7.7e-129
Identity = 216/228 (94.74%), Postives = 221/228 (96.93%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60
           MANEFGESTS PHQNPSWS+NSD TNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR
Sbjct: 1   MANEFGESTSVPHQNPSWSSNSDNTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYR 60

Query: 61  WLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPP 120
           WLHHHSQCQECPVCKAL+QEEKLVPLYGRGK PSDPRLNRYPGIDIP+RPAGQRPQTAPP
Sbjct: 61  WLHHHSQCQECPVCKALVQEEKLVPLYGRGKAPSDPRLNRYPGIDIPNRPAGQRPQTAPP 120

Query: 121 PVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180
           P  NDFANYGFGFTGGFMPMA+ARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG
Sbjct: 121 PAANDFANYGFGFTGGFMPMAAARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 180

Query: 181 YPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           YPYAFNTFHGGHGHHFPQP +RGQHADNVLKNL LLVGVFVILALLWW
Sbjct: 181 YPYAFNTFHGGHGHHFPQPPSRGQHADNVLKNLFLLVGVFVILALLWW 228

[Close]

BLAST of HG10016797 vs. ExPASy TrEMBL
Match: A0A0A0KG01 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G487690 PE=4 SV=1)

HSP 1 Score: 466.5 bits (1199), Expect = 6.5e-128
Identity = 219/229 (95.63%), Postives = 223/229 (97.38%), Query Frame = 0


Query: 1   MANEFGESTSGPHQNPSWSTNSDTT-NNDTGDFECNICFELAQDPIITLCGHLFCWPCLY 60
           MANEFGESTS PHQNPS STNSDTT NNDTGDFECNICFELAQDPIITLCGHLFCWPCLY
Sbjct: 1   MANEFGESTSMPHQNPSCSTNSDTTNNNDTGDFECNICFELAQDPIITLCGHLFCWPCLY 60

Query: 61  RWLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAP 120
           RWLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLN YPG+DIP+RPAGQRPQTAP
Sbjct: 61  RWLHHHSQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNTYPGLDIPNRPAGQRPQTAP 120

Query: 121 PPVPNDFANYGFGFTGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTS 180
           PPVPN+F NYGFGF GGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTS
Sbjct: 121 PPVPNNFPNYGFGFAGGFMPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTS 180

Query: 181 GYPYAFNTFHGGHGHHFPQPTTRGQHADNVLKNLLLLVGVFVILALLWW 229
           GYPYAFNTFHGGHGHHFPQP+TRGQHADNVLKNLLLLVGVFVILALLWW
Sbjct: 181 GYPYAFNTFHGGHGHHFPQPSTRGQHADNVLKNLLLLVGVFVILALLWW 229

[Close]

BLAST of HG10016797 vs. TAIR 10
Match: AT2G23780.1 (RING/U-box superfamily protein )

HSP 1 Score: 273.1 bits (697), Expect = 2.0e-73
Identity = 138/230 (60.00%), Postives = 170/230 (73.91%), Query Frame = 0


Query: 6   GESTSGPHQNPSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHHH 65
           GES++    + S+S +++ TN+  GDFECNICFELAQDPI+TLCGHLFCWPCLYRWLHHH
Sbjct: 4   GESST----STSYSDSNNDTNDQGGDFECNICFELAQDPIVTLCGHLFCWPCLYRWLHHH 63

Query: 66  SQCQECPVCKALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQT-APPPVP- 125
           S  QECPVCKA++Q++KLVPLYGRGK  +DPR  RYPG+ IP+RP GQRP+T APPP P 
Sbjct: 64  SHSQECPVCKAVVQDDKLVPLYGRGKNQTDPRSKRYPGLRIPNRPTGQRPETAAPPPQPE 123

Query: 126 --NDFANYGFGFTGGFMP-MASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYGTTSG 185
             ++F NYG G  GG MP MA+ R GNF++   FGGL+PSLFN QFHGF DAT+YG+T G
Sbjct: 124 AASNFFNYGIGLMGGIMPMMATTRFGNFSM--GFGGLLPSLFNFQFHGFHDATLYGSTPG 183

Query: 186 YPYA--FNTFHGGHGHHFPQPTTR-GQHADNVLKNLLLLVGVFVILALLW 228
           YPY    N F G       +P  R G  +D  LKN+L  VG+ V++ L+W
Sbjct: 184 YPYGGYHNGFRGVPPRGQERPMARGGNQSDAFLKNILFFVGICVVIFLIW 227

[Close]

BLAST of HG10016797 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 268.5 bits (685), Expect = 5.0e-72
Identity = 134/219 (61.19%), Postives = 169/219 (77.17%), Query Frame = 0


Query: 16  PSWSTNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHHHSQCQECPVCK 75
           PS S+ +DT NND+ +FECNIC +LAQDPI+TLCGHLFCWPCLY+WLH HSQ ++CPVCK
Sbjct: 5   PSCSSGNDTNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKDCPVCK 64

Query: 76  ALIQEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPPPVPN-DFA-NYGF-G 135
           A+I+E++LVPLYGRGK  +DPR    PG+++P+RP+GQRP+TA PP PN  FA ++GF G
Sbjct: 65  AVIEEDRLVPLYGRGKSSADPRSKSIPGLEVPNRPSGQRPETAQPPDPNHGFAHHHGFGG 124

Query: 136 FTGGF-MPMASARIGNFTLATAFGGLIPSLFNIQFHGFPDATVYG--TTSGYPYAF-NTF 195
           F GGF  PMASAR GN TL+ AFGGLIPSLFN+ FHGFPDA +YG   + G+P+ F N F
Sbjct: 125 FMGGFAAPMASARFGNVTLSAAFGGLIPSLFNLHFHGFPDAAMYGAAASGGFPHGFSNPF 184

Query: 196 HGGHG--HHFPQPTTRGQHADNVLKNLLLLVGVFVILAL 226
           HGGH   H + + T R    D+ L+ LLL+V V V+ +L
Sbjct: 185 HGGHSHMHSYQRHTGRQGQQDHHLRILLLIVFVVVVFSL 223

[Close]

BLAST of HG10016797 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 141.7 bits (356), Expect = 7.1e-34
Identity = 67/136 (49.26%), Postives = 92/136 (67.65%), Query Frame = 0


Query: 19  STNSDTTNNDTGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHHHSQCQECPVCKALI 78
           +T ++  ++ + +F CNIC ELA++PI+TLCGHLFCWPCLY+WLH+HS+   CPVCKAL+
Sbjct: 4   NTITNEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSNHCPVCKALV 63

Query: 79  QEEKLVPLYGRGKIPSDPRLNRYPGIDIPSRPAGQRPQTAPPPVPNDFANYGFGFTGGFM 138
           +E+ LVPLYG GK  SDPR     G+ +P+RPA  R +TA P +  +  ++G  F GG  
Sbjct: 64  KEDTLVPLYGMGKPSSDPRSKLNSGVTVPNRPAATRTETARPRL--EQRHHGSSFFGGHS 123

Query: 139 PMAS----ARIGNFTL 151
             A+     R  NF L
Sbjct: 124 SFAAMPTGLRFSNFLL 137

[Close]

BLAST of HG10016797 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 98.6 bits (244), Expect = 6.9e-21
Identity = 47/105 (44.76%), Postives = 64/105 (60.95%), Query Frame = 0


Query: 23  DTTNNDT-GDFECNICFELAQDPIITLCGHLFCWPCLYRWLH-------------HHSQC 82
           DTT  D+ GDF+CNIC +  +DP++TLCGHLFCWPC+++W +             H  + 
Sbjct: 9   DTTLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREP 68

Query: 83  QECPVCKALIQEEKLVPLYGRG-KIPSDPRLNRYPGIDIPSRPAG 113
            +CPVCK+ + E  LVP+YGRG K P         G ++PSRP G
Sbjct: 69  PKCPVCKSDVSEATLVPIYGRGQKAPQS-------GSNVPSRPTG 106

[Close]

BLAST of HG10016797 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 96.7 bits (239), Expect = 2.6e-20
Identity = 47/96 (48.96%), Postives = 57/96 (59.38%), Query Frame = 0


Query: 27  NDTGDFECNICFELAQDPIITLCGHLFCWPCLYRWLHHH-------SQCQECPVCKALIQ 86
           N++G F+CNIC + A DP++TLCGHLFCWPC+Y+WLH              CPVCK+ I 
Sbjct: 37  NESGCFDCNICLDTAHDPVVTLCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNIT 96

Query: 87  EEKLVPLYGRG-KIPSD---PRLNRYPGIDIPSRPA 112
              LVPLYGRG   PS     +       DIP RPA
Sbjct: 97  ITSLVPLYGRGMSSPSSTFGSKKQDALSTDIPRRPA 132

The following BLAST results are available for this feature:

BLAST of HG10016797 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881107.1	1.5e-131	97.37	E3 ubiquitin-protein ligase RNF185-like [Benincasa hispida]	[more]
XP_008440158.1	3.4e-131	97.81	PREDICTED: E3 ubiquitin-protein ligase RNF185 [Cucumis melo] >XP_008440160.1 PRE...	[more]
TYK12950.1	4.5e-131	97.37	E3 ubiquitin-protein ligase RNF185 [Cucumis melo var. makuwa]	[more]
XP_022949606.1	1.6e-128	94.74	E3 ubiquitin-protein ligase RNF185-like [Cucurbita moschata] >XP_022978947.1 E3 ...	[more]
KAG6604228.1	1.6e-128	94.74	E3 ubiquitin ligase rnf-5, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of HG10016797 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q09463	1.7e-27	33.47	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]
Q5ZIR9	2.0e-25	43.50	E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1	[more]
Q5RFK9	1.7e-24	41.53	E3 ubiquitin-protein ligase RNF185 OS=Pongo abelii OX=9601 GN=RNF185 PE=2 SV=1	[more]
Q96GF1	3.8e-24	40.98	E3 ubiquitin-protein ligase RNF185 OS=Homo sapiens OX=9606 GN=RNF185 PE=1 SV=1	[more]
Q91YT2	5.0e-24	42.46	E3 ubiquitin-protein ligase RNF185 OS=Mus musculus OX=10090 GN=Rnf185 PE=2 SV=1	[more]

BLAST of HG10016797 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3B021	1.7e-131	97.81	E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo OX=3656 GN=LOC103484707 PE=4 ...	[more]
A0A5D3CNC7	2.2e-131	97.37	E3 ubiquitin-protein ligase RNF185 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1IMG5	7.7e-129	94.74	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita maxima OX=3661 GN=LOC111478...	[more]
A0A6J1GCG3	7.7e-129	94.74	E3 ubiquitin-protein ligase RNF185-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A0A0KG01	6.5e-128	95.63	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G487690 P...	[more]

BLAST of HG10016797 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23780.1	2.0e-73	60.00	RING/U-box superfamily protein	[more]
AT1G19310.1	5.0e-72	61.19	RING/U-box superfamily protein	[more]
AT1G74990.1	7.1e-34	49.26	RING/U-box superfamily protein	[more]
AT4G28270.1	6.9e-21	44.76	RING membrane-anchor 2	[more]
AT4G27470.1	2.6e-20	48.96	RING membrane-anchor 3	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 34..74 e-value: 9.6E-7 score: 38.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 34..75 score: 13.212175
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 15..91 e-value: 1.0E-17 score: 65.7
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 32..79 e-value: 5.2E-8 score: 32.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 94..120
None	No IPR available	PANTHER	PTHR12313:SF47	RING ZINC FINGER PROTEIN-RELATED	coord: 2..227
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 32..76 e-value: 4.15806E-30 score: 103.923
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 30..87
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 2..227
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 49..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10016797.1	HG10016797.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity