HG10016725 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10016725 vs. NCBI nr
Match: XP_038883122.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 716.5 bits (1848), Expect = 1.2e-202
Identity = 334/356 (93.82%), Postives = 345/356 (96.91%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEWVRGDEIGHGSFATINFATPS TY G+QFPPLMAVKSS  VCSASLKNEKQVLDLLGD
Sbjct: 1   MEWVRGDEIGHGSFATINFATPSKTYSGHQFPPLMAVKSSGVVCSASLKNEKQVLDLLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCSYENGEE+YNLFLEYASGGSLADRIQSRGGR+PEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGESCSYENGEEIYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGK RAETGEE GRFQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKKRAETGEEAGRFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESV DNEYESPCDIWAVGCAV+EMLTGKPAWNCR  SNIYSLLLKIGIGEELPEIPK
Sbjct: 181 MSPESVKDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNIYSLLLKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFED 300
           DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFV+DSGR+VPLT +DEPSTSPRCPFDFED
Sbjct: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVADSGRTVPLTAVDEPSTSPRCPFDFED 300

Query: 301 WASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           WASIHSQEADQRFEEESNCWLNSSCSWLS P+ERLLQLVDNGPV+WSVT++WVTVR
Sbjct: 301 WASIHSQEADQRFEEESNCWLNSSCSWLSSPRERLLQLVDNGPVDWSVTNNWVTVR 356

BLAST of HG10016725 vs. NCBI nr
Match: XP_008440288.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo] >TYK12881.1 mitogen-activated protein kinase kinase kinase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 652.1 bits (1681), Expect = 2.7e-183
Identity = 308/357 (86.27%), Postives = 327/357 (91.60%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+WVRG EIGHGSFATIN ATPS+   G++FPPLMAVKSS  VCS+SLKNEKQ+LDLLGD
Sbjct: 1   MDWVRGHEIGHGSFATINLATPSS---GDRFPPLMAVKSSELVCSSSLKNEKQILDLLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS ENGEELYNLFLEYA+GGSLADRIQS GGR+PEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNR ETGEE GRFQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPES+NDNEYESPCDIWAVGCAV+EM TGKPAWNCR  SN+++LL+KIGIGEELPEIPK
Sbjct: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFED 300
           DLSEEGKDFL KCLVKDP KRWTADMLLKHPFV+DSGR VPL  MDE STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSQEADQRFEEESNCWLNSSCSW-LSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           WASIHSQE D R EEESNCWLNSSCSW  S P+ERLLQLV+ G V+WSVTD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of HG10016725 vs. NCBI nr
Match: XP_011657865.1 (mitogen-activated protein kinase kinase kinase 17 [Cucumis sativus] >KGN48531.1 hypothetical protein Csa_004223 [Cucumis sativus])

HSP 1 Score: 636.0 bits (1639), Expect = 2.0e-178
Identity = 300/356 (84.27%), Postives = 323/356 (90.73%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+WVRGDEIG+GSFATIN AT  +   G++FPPLMAVKSS  VCSASLKNEKQVLDLLGD
Sbjct: 1   MDWVRGDEIGYGSFATINLATSCS---GDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFG+SCS ENGEELYNLFLEYASGGSLADRIQSRGGR+PEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNR  TGEE G+FQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESVNDNEYESPCDIWAVGCAV+EMLTGKPAWNCR  SN+++LL+KIGIGEELPEIPK
Sbjct: 181 MSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFED 300
           DLSEEGKDFL KCLVKDP KRWTADMLLKHPFV++SGR VPL  ++E STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESGRCVPLAGVEEVSTSPRCPFDFED 300

Query: 301 WASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           WASIHSQE+D R EEE+NCWLN   +W   P+ERLL+LV NG V WSVTD+WV VR
Sbjct: 301 WASIHSQESDPRNEEEANCWLN---NWSCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of HG10016725 vs. NCBI nr
Match: XP_023517683.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo] >XP_023522778.1 mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 627.5 bits (1617), Expect = 7.1e-176
Identity = 305/357 (85.43%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+W+RG EIG GSFATIN A PSNT    QFPPLMAVKSS AV SASLKNEK+VLDLLG 
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNTSTA-QFPPLMAVKSSPAVSSASLKNEKRVLDLLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS E G+ELYNLFLEYASGGSLADRIQS GG +PEFEVRR TRTILEG
Sbjct: 61  DCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIH KGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNRAETGEE G+FQLRGSPLY
Sbjct: 121 LRYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESVNDNEYESPCDIWAVGCAV+EMLTGKPAWN    SNIYSLLLKIGIGEELP+IPK
Sbjct: 181 MSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSG-RSVPLTTMDEPSTSPRCPFDFE 300
            +SEEGKDFLGKCLVKDPAKRWTA+MLL HPF++DSG  SVPLT  DEPSTSPRCPFDFE
Sbjct: 241 YISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFE 300

Query: 301 DWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           DWASIHSQE DQ+FEEESNCWL+SSC W   P ERLLQLVDNGPV+W +TDSWVTVR
Sbjct: 301 DWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of HG10016725 vs. NCBI nr
Match: KAG7027055.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 626.7 bits (1615), Expect = 1.2e-175
Identity = 304/357 (85.15%), Postives = 321/357 (89.92%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+W+RG EIG GSFATIN A PSNT    QFPPLMAVKSS AV SASLKNEK+VLDLLG 
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNT-SNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS E G+ELYNLFLEYASGGSLADRIQS GG +PEFEVRR TRTILEG
Sbjct: 61  DCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIH KGFVHCDVKPRNILVF DG+AKIADFGLSKKAGKNRAETGEE G+FQLRGSPLY
Sbjct: 121 LRYIHDKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESVNDNEYESPCDIWAVGCAV+EMLTGKPAWN    SNIYSLLLKIGIGEELP+IPK
Sbjct: 181 MSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFSMESNIYSLLLKIGIGEELPKIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSG-RSVPLTTMDEPSTSPRCPFDFE 300
            +SEEGKDFLGKCLVKDPAKRWTA+MLL HPF++DSG  SVPLT  DEPSTSPRCPFDFE
Sbjct: 241 YISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFE 300

Query: 301 DWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           DWASIHSQE DQ+FEEESNCWL+SSC W   P ERLLQLVDNGPV+W +TDSWVTVR
Sbjct: 301 DWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of HG10016725 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 9.3e-102
Identity = 192/364 (52.75%), Postives = 248/364 (68.13%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEWVRG+ IG G+F+T++ AT S       FP L+AVKS+ A  +ASL NEK VLD LG 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRN--SGDFPALIAVKSTDAYGAASLSNEKSVLDSLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGR-IPEFEVRRYTRTILE 120
           DCP+IIRC+GE  + ENGEE++NL LEYAS GSLA  ++  GG  +PE  VRR+T ++L 
Sbjct: 61  DCPEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 120

Query: 121 GLRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPL 180
           GLR+IH KGF HCD+K  NIL+F+DG  KIADFGL+ +   +     + V   ++RG+PL
Sbjct: 121 GLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSV---EIRGTPL 180

Query: 181 YMSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIP 240
           YM+PE VNDNEY S  D+WA+GCAV+EM +GK AW+ ++GS+  SLL++IG+G+ELP+IP
Sbjct: 181 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIP 240

Query: 241 KDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVS----DSGRS---VPLTTMDEPSTSP 300
           + LSEEGKDFL KC VKDPAKRWTA+MLL H FV+    D  R    V +   D+   SP
Sbjct: 241 EMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSP 300

Query: 301 RCPFDFEDWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSW 357
           +CPF+F+DW S         F  +SN   +S       P ERL  LV     +WSV  SW
Sbjct: 301 KCPFEFDDWDS---------FTLDSNPSFDS-------PVERLGSLVSGSIPDWSVGGSW 342

BLAST of HG10016725 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 8.8e-60
Identity = 135/362 (37.29%), Postives = 189/362 (52.21%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEW RG  +G GS AT+  A       G+    ++AVKSS    S  L+ E ++L  L  
Sbjct: 1   MEWTRGRILGRGSTATVYAA------AGHNSDEILAVKSSEVHRSEFLQREAKILSSLSS 60

Query: 61  DCPQIIRCFGESCSYE-NGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILE 120
             P +I   G     E NG  +YNL +EYA  G+L D     GGR+ E  V +YTR IL+
Sbjct: 61  --PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILK 120

Query: 121 GLRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPL 180
           GL YIH KG VHCDVK  N+++ + G+AKIADFG +K+         + V    + G+P 
Sbjct: 121 GLEYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKRV--------DPVFESPVMGTPA 180

Query: 181 YMSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAW-NCRQGSNIYSLLLKIGIGEELPEI 240
           +M+PE     +     DIWAVGC +IEM+TG P W       +  S+L ++G   E PE+
Sbjct: 181 FMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPEL 240

Query: 241 PKDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDF 300
           P  L+EE KDFL KCL ++  +RWTA  LL HPF++      P+      S SP    D 
Sbjct: 241 PCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQ 300

Query: 301 EDWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTD----SWVT 357
             W S+  +E ++  E + +       S   C  ER+ +L   G ++ +  D     W+ 
Sbjct: 301 TFWRSVEEEEEEETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIM 346

BLAST of HG10016725 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 1.6e-53
Identity = 138/368 (37.50%), Postives = 189/368 (51.36%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M W RG  +G GS AT++ AT     C ++    +AVKS+    S  L+ E ++L  L  
Sbjct: 1   MNWTRGKTLGRGSTATVSAAT-----C-HESGETLAVKSAEFHRSEFLQREAKILSSL-- 60

Query: 61  DCPQIIRCFGESCSYE----NGE-ELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTR 120
           + P +I   G   + E    NGE   Y+L +EYA  G+L D     GG I E  V +YTR
Sbjct: 61  NSPYVIGYRGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTR 120

Query: 121 TILEGLRYIH-GKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQL 180
            IL GL YIH  KG  HCD+K  N+LV ++G+AKIADFG +K       E         +
Sbjct: 121 QILLGLEYIHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITE--------PV 180

Query: 181 RGSPLYMSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEE 240
           RG+P +M+PE+          DIWAVGC VIEM+TG   W     ++  S+L ++G   E
Sbjct: 181 RGTPAFMAPEAARGERQGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGE 240

Query: 241 LPEIPKDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRC 300
           LPE+P  L+E+ KDFLGKCL K+  +RWTA  LL HPF+ +     P       + SP  
Sbjct: 241 LPELPCSLTEQAKDFLGKCLKKEATERWTASQLLNHPFLVN---KEPELVTGLVTNSPTS 300

Query: 301 PFDFEDWASIHSQEADQRFEEESNCWLNSSCSWLSCPK-ERLLQLVDNGPV----NWSVT 357
             D   W S+  + ++ R             SW  C + ER+  L   G V     W + 
Sbjct: 301 VTDQMFWRSVEEEVSEDR------------SSWWECHEDERIGVLSWIGHVVVESTWDLD 337

BLAST of HG10016725 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.2e-42
Identity = 98/280 (35.00%), Postives = 153/280 (54.64%), Query Frame = 0

Query: 3   WVRGDEIGHGSFATI--NFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 62
           W +G  +G G+F  +   F +     C  +   +++   ++  C   LK   Q ++LL  
Sbjct: 214 WKKGKFLGSGTFGQVYLGFNSEKGKMCAIKEVKVISDDQTSKEC---LKQLNQEINLLNQ 273

Query: 63  DC-PQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILE 122
            C P I++ +G   S    EE  +++LEY SGGS+   ++  G    E  ++ YTR IL 
Sbjct: 274 LCHPNIVQYYGSELS----EETLSVYLEYVSGGSIHKLLKDYGS-FTEPVIQNYTRQILA 333

Query: 123 GLRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPL 182
           GL Y+HG+  VH D+K  NILV  +G+ K+ADFG++K                  +GSP 
Sbjct: 334 GLAYLHGRNTVHRDIKGANILVDPNGEIKLADFGMAKHVTAFST-------MLSFKGSPY 393

Query: 183 YMSPESV-NDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEI 242
           +M+PE V + N Y    DIW++GC ++EM T KP W+  +G    + + KIG  ++ PEI
Sbjct: 394 WMAPEVVMSQNGYTHAVDIWSLGCTILEMATSKPPWSQFEG---VAAIFKIGNSKDTPEI 453

Query: 243 PKDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGR 279
           P  LS + K+F+  CL ++P  R TA  LL+HPF+ ++ R
Sbjct: 454 PDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFLRNTTR 475

BLAST of HG10016725 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.2e-42
Identity = 104/274 (37.96%), Postives = 145/274 (52.92%), Query Frame = 0

Query: 3   WVRGDEIGHGSFATI--NFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 62
           W +G  +G GSF  +   F + S   C  +   L +    +   +  L  E  VL  L  
Sbjct: 400 WKKGRLLGMGSFGHVYLGFNSESGEMCAMKEVTLCSDDPKSRESAQQLGQEISVLSRLRH 459

Query: 63  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 122
               I++ +G     E  ++   ++LEY SGGS+   +Q   G+  E  +R YT+ IL G
Sbjct: 460 Q--NIVQYYGS----ETVDDKLYIYLEYVSGGSIYKLLQEY-GQFGENAIRNYTQQILSG 519

Query: 123 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 182
           L Y+H K  VH D+K  NILV   G  K+ADFG++K           + G    +GSP +
Sbjct: 520 LAYLHAKNTVHRDIKGANILVDPHGRVKVADFGMAK-------HITAQSGPLSFKGSPYW 579

Query: 183 MSPESV-NDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIP 242
           M+PE + N N      DIW++GC V+EM T KP W+  +G      + KIG  +ELP+IP
Sbjct: 580 MAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEG---VPAMFKIGNSKELPDIP 639

Query: 243 KDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFV 274
             LSEEGKDF+ KCL ++PA R TA  LL H FV
Sbjct: 640 DHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

BLAST of HG10016725 vs. ExPASy TrEMBL
Match: A0A5D3CM36 (Mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004750 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.3e-183
Identity = 308/357 (86.27%), Postives = 327/357 (91.60%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+WVRG EIGHGSFATIN ATPS+   G++FPPLMAVKSS  VCS+SLKNEKQ+LDLLGD
Sbjct: 1   MDWVRGHEIGHGSFATINLATPSS---GDRFPPLMAVKSSELVCSSSLKNEKQILDLLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS ENGEELYNLFLEYA+GGSLADRIQS GGR+PEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNR ETGEE GRFQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPES+NDNEYESPCDIWAVGCAV+EM TGKPAWNCR  SN+++LL+KIGIGEELPEIPK
Sbjct: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFED 300
           DLSEEGKDFL KCLVKDP KRWTADMLLKHPFV+DSGR VPL  MDE STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSQEADQRFEEESNCWLNSSCSW-LSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           WASIHSQE D R EEESNCWLNSSCSW  S P+ERLLQLV+ G V+WSVTD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of HG10016725 vs. ExPASy TrEMBL
Match: A0A1S3B1I0 (mitogen-activated protein kinase kinase kinase 3-like OS=Cucumis melo OX=3656 GN=LOC103484781 PE=3 SV=1)

HSP 1 Score: 652.1 bits (1681), Expect = 1.3e-183
Identity = 308/357 (86.27%), Postives = 327/357 (91.60%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+WVRG EIGHGSFATIN ATPS+   G++FPPLMAVKSS  VCS+SLKNEKQ+LDLLGD
Sbjct: 1   MDWVRGHEIGHGSFATINLATPSS---GDRFPPLMAVKSSELVCSSSLKNEKQILDLLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS ENGEELYNLFLEYA+GGSLADRIQS GGR+PEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGESCSVENGEELYNLFLEYATGGSLADRIQSCGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNR ETGEE GRFQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVETGEETGRFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPES+NDNEYESPCDIWAVGCAV+EM TGKPAWNCR  SN+++LL+KIGIGEELPEIPK
Sbjct: 181 MSPESLNDNEYESPCDIWAVGCAVVEMFTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFED 300
           DLSEEGKDFL KCLVKDP KRWTADMLLKHPFV+DSGR VPL  MDE STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLRKCLVKDPVKRWTADMLLKHPFVADSGRCVPLGEMDEVSTSPRCPFDFED 300

Query: 301 WASIHSQEADQRFEEESNCWLNSSCSW-LSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           WASIHSQE D R EEESNCWLNSSCSW  S P+ERLLQLV+ G V+WSVTD+WV VR
Sbjct: 301 WASIHSQERDPRNEEESNCWLNSSCSWSSSSPRERLLQLVEKGAVDWSVTDNWVRVR 354

BLAST of HG10016725 vs. ExPASy TrEMBL
Match: A0A0A0KLC9 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490950 PE=3 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 9.7e-179
Identity = 300/356 (84.27%), Postives = 323/356 (90.73%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+WVRGDEIG+GSFATIN AT  +   G++FPPLMAVKSS  VCSASLKNEKQVLDLLGD
Sbjct: 1   MDWVRGDEIGYGSFATINLATSCS---GDRFPPLMAVKSSGLVCSASLKNEKQVLDLLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFG+SCS ENGEELYNLFLEYASGGSLADRIQSRGGR+PEFEVRRYTRTILEG
Sbjct: 61  DCPQIIRCFGDSCSVENGEELYNLFLEYASGGSLADRIQSRGGRLPEFEVRRYTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIHGKGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNR  TGEE G+FQLRGSPLY
Sbjct: 121 LRYIHGKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRVGTGEETGKFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESVNDNEYESPCDIWAVGCAV+EMLTGKPAWNCR  SN+++LL+KIGIGEELPEIPK
Sbjct: 181 MSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNCRPESNVFALLIKIGIGEELPEIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFED 300
           DLSEEGKDFL KCLVKDP KRWTADMLLKHPFV++SGR VPL  ++E STSPRCPFDFED
Sbjct: 241 DLSEEGKDFLKKCLVKDPLKRWTADMLLKHPFVAESGRCVPLAGVEEVSTSPRCPFDFED 300

Query: 301 WASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           WASIHSQE+D R EEE+NCWLN   +W   P+ERLL+LV NG V WSVTD+WV VR
Sbjct: 301 WASIHSQESDPRNEEEANCWLN---NWSCSPRERLLELVGNGAVEWSVTDNWVRVR 350

BLAST of HG10016725 vs. ExPASy TrEMBL
Match: A0A6J1HG32 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111463177 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 5.0e-175
Identity = 303/357 (84.87%), Postives = 320/357 (89.64%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           M+W+RG EIG GSFATIN A PSNT    QFPPLMAVKSS AV SASLKNEK+VLDLLG 
Sbjct: 1   MDWIRGHEIGCGSFATINLAMPSNT-SNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS E G+ELYNLFLEYASGGSLADRIQS GG +PEFEVRR TRTILEG
Sbjct: 61  DCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRLTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIH KGFVHCDVKPRNILVF DG+AKIADFGLSKKAGKNRAETGEE G+FQLRGSPLY
Sbjct: 121 LRYIHEKGFVHCDVKPRNILVFGDGEAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESVNDNEYESPCDIWAVGCAV+EMLTGKPAWN    SNIYSLLLKIGIGEELP+IPK
Sbjct: 181 MSPESVNDNEYESPCDIWAVGCAVVEMLTGKPAWNFPMESNIYSLLLKIGIGEELPKIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSG-RSVPLTTMDEPSTSPRCPFDFE 300
            +SEEGKDFLGKCLVKDPAKRWTA+MLL HPF++DSG  SVPLT  DEPSTSPRCPFDFE
Sbjct: 241 YISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKDEPSTSPRCPFDFE 300

Query: 301 DWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           DWASIHSQE DQ+FEEESNCWL+SSC W   P ERLLQLVD GPV+W +TDSWVTVR
Sbjct: 301 DWASIHSQEVDQKFEEESNCWLDSSCPWSCSPTERLLQLVDKGPVDWVITDSWVTVR 354

BLAST of HG10016725 vs. ExPASy TrEMBL
Match: A0A6J1KQ57 (mitogen-activated protein kinase kinase kinase 18-like OS=Cucurbita maxima OX=3661 GN=LOC111497247 PE=3 SV=1)

HSP 1 Score: 617.5 bits (1591), Expect = 3.6e-173
Identity = 301/357 (84.31%), Postives = 318/357 (89.08%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEW+RG EIG GSFATIN A PSNT    QFPPLMAVKSS AV SASLKNEK+VLDLLG 
Sbjct: 1   MEWIRGHEIGCGSFATINLAMPSNT-SNAQFPPLMAVKSSPAVSSASLKNEKRVLDLLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DCPQIIRCFGESCS E G+ELYNLFLEYASGGSLADRIQS GG +PEFEVRR+TRTILEG
Sbjct: 61  DCPQIIRCFGESCSVERGDELYNLFLEYASGGSLADRIQSHGGWLPEFEVRRFTRTILEG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           LRYIH KGFVHCDVKPRNILVF DGDAKIADFGLSKKAGKNRAETGEE G+FQLRGSPLY
Sbjct: 121 LRYIHEKGFVHCDVKPRNILVFGDGDAKIADFGLSKKAGKNRAETGEE-GKFQLRGSPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPK 240
           MSPESVND+EYESPCDIWAVGCAV+EMLTGKPAWN     NIYSLLLKIGIGEELP+IPK
Sbjct: 181 MSPESVNDDEYESPCDIWAVGCAVVEMLTGKPAWNFPVECNIYSLLLKIGIGEELPKIPK 240

Query: 241 DLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSG-RSVPLTTMDEPSTSPRCPFDFE 300
            +SEEGKDFLGKCLVKDPAKRWTA+MLL HPF++DSG  SVPLT   EP TSPRCPFDFE
Sbjct: 241 YISEEGKDFLGKCLVKDPAKRWTAEMLLNHPFLADSGSSSVPLTPKGEPLTSPRCPFDFE 300

Query: 301 DWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           DWASIHS E DQ+FEEESNCWL+SSC W   P ERLLQLVDNGPV+W +TDSWVTVR
Sbjct: 301 DWASIHSLEMDQKFEEESNCWLDSSCPWSCSPTERLLQLVDNGPVDWVITDSWVTVR 354

BLAST of HG10016725 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 388.3 bits (996), Expect = 6.8e-108
Identity = 195/366 (53.28%), Postives = 252/366 (68.85%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEW+RG+ IG+G+F+T++ AT SN   G +FPPLMAVKS+ +  +ASL NEK VLD LGD
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSG-EFPPLMAVKSADSYGAASLANEKSVLDNLGD 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGR-IPEFEVRRYTRTILE 120
           DC +I+RCFGE  + ENGEE++NLFLEYAS GSL   ++   G  +PE  VRR+T ++L 
Sbjct: 61  DCNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLR 120

Query: 121 GLRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPL 180
           GLR+IH  GF HCD+K  NIL+F DG  KIADFGL+K+ G     T    G  Q+RG+PL
Sbjct: 121 GLRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGD---LTALNYG-VQIRGTPL 180

Query: 181 YMSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIP 240
           YM+PESVNDNEY S  D+WA+GC V+EM +GK AW+ ++GSN  SLLL+IG+G+E+P IP
Sbjct: 181 YMAPESVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIP 240

Query: 241 KDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVS-DSGRSVPLTTMD--------EPST 300
           ++LSE+G+DFL KC VKDP KRWTA+MLL HPFV+ D    V +   D        + ST
Sbjct: 241 EELSEQGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVST 300

Query: 301 SPRCPFDFEDWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTD 357
           SPRCPF+F DW S+                 +S    +  P ER+  LV +   +WSVT+
Sbjct: 301 SPRCPFEFPDWVSV-----------------SSGSQTIDSPDERVASLVTDMIPDWSVTN 344

BLAST of HG10016725 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 381.7 bits (979), Expect = 6.4e-106
Identity = 195/359 (54.32%), Postives = 249/359 (69.36%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEW+R + IGHGSF+T++ AT S +     FP LMAVKSS  VCSA+L+NE+ VLD LG 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGS-SSKAFPSLMAVKSSGVVCSAALRNERDVLDDLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEG 120
           DC +I+RCFGE  + ENGEE+YNLFLEYASGGSLADRI+S G  +PEFEVRR+TR+I++G
Sbjct: 61  DCSEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKG 120

Query: 121 LRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLY 180
           L +IHG GF HCD+K  N+LVF DGD KI+DFGL+K+       +GE     ++RG+PLY
Sbjct: 121 LCHIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKR------RSGEVC--VEIRGTPLY 180

Query: 181 MSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQG--SNIYSLLLKIGIGEELPEI 240
           M+PESVN  E+ESP DIWA+GC+V+EM +GK AW    G  +N+ SLL++IG G+E+P I
Sbjct: 181 MAPESVNHGEFESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRI 240

Query: 241 PKDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEP-STSPRCPFD 300
           P +LSEEGKDF+ KC VK+ A+RWTA+MLL HPF++    S      DE  S SPR PFD
Sbjct: 241 PVELSEEGKDFVSKCFVKNAAERWTAEMLLDHPFLAVDDES---GEEDEACSVSPRNPFD 300

Query: 301 FEDWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSWVTVR 357
           F  W S+ S   D               S +  P+ER+  LV     +WSV+  WV VR
Sbjct: 301 FPGWNSVQSPVNDSVM----------FGSLVGSPEERISGLVSEKVPDWSVSCDWVNVR 336

BLAST of HG10016725 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 371.7 bits (953), Expect = 6.6e-103
Identity = 192/364 (52.75%), Postives = 248/364 (68.13%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEWVRG+ IG G+F+T++ AT S       FP L+AVKS+ A  +ASL NEK VLD LG 
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRN--SGDFPALIAVKSTDAYGAASLSNEKSVLDSLG- 60

Query: 61  DCPQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGR-IPEFEVRRYTRTILE 120
           DCP+IIRC+GE  + ENGEE++NL LEYAS GSLA  ++  GG  +PE  VRR+T ++L 
Sbjct: 61  DCPEIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLR 120

Query: 121 GLRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPL 180
           GLR+IH KGF HCD+K  NIL+F+DG  KIADFGL+ +   +     + V   ++RG+PL
Sbjct: 121 GLRHIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSV---EIRGTPL 180

Query: 181 YMSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIP 240
           YM+PE VNDNEY S  D+WA+GCAV+EM +GK AW+ ++GS+  SLL++IG+G+ELP+IP
Sbjct: 181 YMAPECVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIP 240

Query: 241 KDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVS----DSGRS---VPLTTMDEPSTSP 300
           + LSEEGKDFL KC VKDPAKRWTA+MLL H FV+    D  R    V +   D+   SP
Sbjct: 241 EMLSEEGKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSP 300

Query: 301 RCPFDFEDWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTDSW 357
           +CPF+F+DW S         F  +SN   +S       P ERL  LV     +WSV  SW
Sbjct: 301 KCPFEFDDWDS---------FTLDSNPSFDS-------PVERLGSLVSGSIPDWSVGGSW 342

BLAST of HG10016725 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 234.6 bits (597), Expect = 1.3e-61
Identity = 138/361 (38.23%), Postives = 201/361 (55.68%), Query Frame = 0

Query: 3   WVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGDDC 62
           W+RG  IG GS AT++      T  G+ F    AVKS+    SA L+ E+ +L  L    
Sbjct: 6   WIRGPIIGRGSTATVSLGI---TNSGDFF----AVKSAEFSSSAFLQREQSILSKLSS-- 65

Query: 63  PQIIRCFGESCSYENGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILEGLR 122
           P I++  G + + EN + +YNL +EY SGGSL D I++ GG++PE  +R YTR IL+GL 
Sbjct: 66  PYIVKYIGSNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLM 125

Query: 123 YIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPLYMS 182
           Y+H +G VHCDVK +N+++  +  AKI D G +K        T EE    +  G+P +MS
Sbjct: 126 YLHDQGIVHCDVKSQNVMIGGE-IAKIVDLGCAK--------TVEENENLEFSGTPAFMS 185

Query: 183 PESVNDNEYESPCDIWAVGCAVIEMLTGKPAWNCRQGSNIYSLLLKIGIGEELPEIPKDL 242
           PE     E   P D+WA+GC VIEM TG   W   + +++ + + KIG   E P IP  L
Sbjct: 186 PEVARGEEQSFPADVWALGCTVIEMATGSSPW--PELNDVVAAIYKIGFTGESPVIPVWL 245

Query: 243 SEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDFEDWA 302
           SE+G+DFL KCL KDP +RWT + LL+HPF+ +       T     S+SP    D   W 
Sbjct: 246 SEKGQDFLRKCLRKDPKQRWTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWD 305

Query: 303 SIHSQEADQRFEEESNCWLNSSCSWL----SCPKERLLQLV---DNGPVNWSVTDSWVTV 357
              +  +    E+  + + NS+ ++L    S P +R+ +L     +G  +W  T+ W+ V
Sbjct: 306 LCETSRSRFIKEDHEDPFANST-NFLWDDDSLPGDRIKKLAGDESSGEPDWE-TNGWIEV 344

BLAST of HG10016725 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 232.3 bits (591), Expect = 6.2e-61
Identity = 135/362 (37.29%), Postives = 189/362 (52.21%), Query Frame = 0

Query: 1   MEWVRGDEIGHGSFATINFATPSNTYCGNQFPPLMAVKSSAAVCSASLKNEKQVLDLLGD 60
           MEW RG  +G GS AT+  A       G+    ++AVKSS    S  L+ E ++L  L  
Sbjct: 1   MEWTRGRILGRGSTATVYAA------AGHNSDEILAVKSSEVHRSEFLQREAKILSSLSS 60

Query: 61  DCPQIIRCFGESCSYE-NGEELYNLFLEYASGGSLADRIQSRGGRIPEFEVRRYTRTILE 120
             P +I   G     E NG  +YNL +EYA  G+L D     GGR+ E  V +YTR IL+
Sbjct: 61  --PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILK 120

Query: 121 GLRYIHGKGFVHCDVKPRNILVFDDGDAKIADFGLSKKAGKNRAETGEEVGRFQLRGSPL 180
           GL YIH KG VHCDVK  N+++ + G+AKIADFG +K+         + V    + G+P 
Sbjct: 121 GLEYIHSKGIVHCDVKGSNVVISEKGEAKIADFGCAKRV--------DPVFESPVMGTPA 180

Query: 181 YMSPESVNDNEYESPCDIWAVGCAVIEMLTGKPAW-NCRQGSNIYSLLLKIGIGEELPEI 240
           +M+PE     +     DIWAVGC +IEM+TG P W       +  S+L ++G   E PE+
Sbjct: 181 FMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPEL 240

Query: 241 PKDLSEEGKDFLGKCLVKDPAKRWTADMLLKHPFVSDSGRSVPLTTMDEPSTSPRCPFDF 300
           P  L+EE KDFL KCL ++  +RWTA  LL HPF++      P+      S SP    D 
Sbjct: 241 PCLLAEEAKDFLEKCLKREANERWTATQLLNHPFLTTKPDIEPVLVPGLISNSPTSVTDQ 300

Query: 301 EDWASIHSQEADQRFEEESNCWLNSSCSWLSCPKERLLQLVDNGPVNWSVTD----SWVT 357
             W S+  +E ++  E + +       S   C  ER+ +L   G ++ +  D     W+ 
Sbjct: 301 TFWRSVEEEEEEETEEIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIM 346