HG10016681 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10016681 vs. NCBI nr
Match: XP_038881674.1 (subtilisin-like protease SBT1.7 [Benincasa hispida])

HSP 1 Score: 1484.5 bits (3842), Expect = 0.0e+00
Identity = 744/762 (97.64%), Postives = 751/762 (98.56%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60
           MANP+WIFLL CFFSVPSMAAAQ KKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE
Sbjct: 1   MANPLWIFLLLCFFSVPSMAAAQ-KKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120
           MIYAYNNVVHGFSTRLTAEEAQRLE+QPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP
Sbjct: 61  MIYAYNNVVHGFSTRLTAEEAQRLESQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120

Query: 121 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
           ESNSVSEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKALP 360

Query: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 480
           VLANT ANGEELVADSHLLPATAVGQKSGDI+RKYLISEPNPTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIMRKYLISEPNPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGL IDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLPIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAY+NGQKIQDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYRNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660
           P SALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720
           VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720

Query: 721 SYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           SYTVTFT   GSAAP SAEGFGRIEWSDGK VVGSPIAFSWT
Sbjct: 721 SYTVTFTTVTGSAAPPSAEGFGRIEWSDGKQVVGSPIAFSWT 761

BLAST of HG10016681 vs. NCBI nr
Match: XP_008440346.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo] >TYK12836.1 subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 732/762 (96.06%), Postives = 748/762 (98.16%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60
           MANPVW FLL C FSVPSMA   KKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120
           MIYAYNNVVHGFSTRLT EEAQRLEAQPGILAVVPEMRYELHTTR+PQFLGLDKNANLYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 121 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
           ESNSVSEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 480
           VLANT ANGEELVADSHLLPATAVGQKSGDIIRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660
           P SALNPGLVYDLTVDDYLNFLCALNYTPSQINSLAR+DFTCDSKKKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720
           VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFTGANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 721 SYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           SYTVTFT + GSAAP+SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTFT-SNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of HG10016681 vs. NCBI nr
Match: XP_004141887.1 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KGN48584.1 hypothetical protein Csa_004174 [Cucumis sativus])

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 725/762 (95.14%), Postives = 744/762 (97.64%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60
           MANPVW+FLL CFFSVPSMA    KKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSD+AE
Sbjct: 1   MANPVWMFLLLCFFSVPSMAVGD-KKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120
           MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEM YELHTTR+P+FLGLDKNANLYP
Sbjct: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMIYELHTTRSPEFLGLDKNANLYP 120

Query: 121 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
           ESNSVSEVIIGVLDTGI PESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKL+GARF
Sbjct: 121 ESNSVSEVIIGVLDTGISPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLVGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AAI+KAV+DNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAIDKAVDDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 480
           VLANT ANGEELVADSHLLPATAVGQKSGD IRKYL+S+P+PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDTIRKYLVSDPSPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660
           P SALNPGLVYDLTVDDYLNFLCALNYTPSQINSLAR+DFTCDSKKKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720
           VF+GVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESLSFTGAN+KK
Sbjct: 661 VFEGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKK 720

Query: 721 SYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           SYTVTFT    SAAP SAE FGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTFTTTTSSAAPTSAEAFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of HG10016681 vs. NCBI nr
Match: KAG6604148.1 (Subtilisin-like protease 1.7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1430.2 bits (3701), Expect = 0.0e+00
Identity = 714/764 (93.46%), Postives = 738/764 (96.60%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAA--AQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDT 60
           M  PVWIFLLFCFFS P MAA  A +KKTY+VHMAKYQMP++FEHHLHWYD+SL+SVSDT
Sbjct: 1   MEKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANL 120
           A+MIYAY  VVHGFSTRLTAEEAQRLEAQPG+LAVVPEMRYELHTTRTPQFLGLDKNA+L
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPES S SEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GD KNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 361 LPGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           LPGTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 480
           GMVLANT ANGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSF 660
           VDP SALNPGLVYDLTVDDYL+FLCALNYT SQINSLARRD+TCDS KKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANE 720
           AVVFDGVLGGG SGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           KKSYTVTFT A GS+AP SAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of HG10016681 vs. NCBI nr
Match: XP_023543760.1 (subtilisin-like protease SBT1.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1429.1 bits (3698), Expect = 0.0e+00
Identity = 712/763 (93.32%), Postives = 738/763 (96.72%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSM-AAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTA 60
           M  PVW+FLLFCFFS P M AAA +KKTY+VHMAKYQMP++FEHHLHWYD+SL+SVSDTA
Sbjct: 1   MEKPVWVFLLFCFFSGPFMAAAAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDTA 60

Query: 61  EMIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLY 120
           +MIYAY  VVHGFSTRLTAEEAQRLEAQPG+LAVVPEMRYELHTTRTPQFLGLDKNA+LY
Sbjct: 61  DMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADLY 120

Query: 121 PESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180
           PES S SEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR
Sbjct: 121 PESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGAR 180

Query: 181 FFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240
           FFS+GYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR
Sbjct: 181 FFSRGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 240

Query: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEK 300
           ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAMEK
Sbjct: 241 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAMEK 300

Query: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPL 360
           GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GD KNFSGVSLYRGK L
Sbjct: 301 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKSL 360

Query: 361 PGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 420
           PGTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIG
Sbjct: 361 PGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIG 420

Query: 421 MVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSP 480
           MVLANT ANGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPSP
Sbjct: 421 MVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPSP 480

Query: 481 VVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMS 540
           VVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSMS
Sbjct: 481 VVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSMS 540

Query: 541 CPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 600
           CPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV
Sbjct: 541 CPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 600

Query: 601 DPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFA 660
           DP SALNPGLVYDLTVDDYL+FLCALNYT SQINSLARRD+TCDS KKYSVN+LNYPSFA
Sbjct: 601 DPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSFA 660

Query: 661 VVFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEK 720
           VVFDGVLGGG SGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANEK
Sbjct: 661 VVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANEK 720

Query: 721 KSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           KSYTVTFT A GS+AP SAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KSYTVTFTTATGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 763

BLAST of HG10016681 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 545/756 (72.09%), Postives = 633/756 (83.73%), Query Frame = 0

Query: 8   FLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEMIYAYNN 67
           FLL C       +++  + TYIVHMAK QMP SF+ H +WYDSSLRS+SD+AE++Y Y N
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYEN 71

Query: 68  VVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKN-ANLYPESNSVS 127
            +HGFSTRLT EEA  L  QPG+++V+PE RYELHTTRTP FLGLD++ A+L+PE+ S S
Sbjct: 72  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 131

Query: 128 EVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 187
           +V++GVLDTG+WPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGARFF++GYE
Sbjct: 132 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 191

Query: 188 ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYK 247
           +T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RARVA YK
Sbjct: 192 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 251

Query: 248 VCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCS 307
           VCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+GIL+SCS
Sbjct: 252 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 311

Query: 308 AGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLPGTLLPF 367
           AGNAGPS  SLSN +PWITTVGAGTLDRDFPA   LG+ KNF+GVSL++G+ LP  LLPF
Sbjct: 312 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 371

Query: 368 IYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTP 427
           IYA NASN+ NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM+LANT 
Sbjct: 372 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 431

Query: 428 ANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSS 487
           ANGEELVAD+HLLPAT VG+K+GDIIR Y+ ++PNPT +I   GT +G++PSPVVAAFSS
Sbjct: 432 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSS 491

Query: 488 RGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGL 547
           RGPNSITP +LKPD+IAPGVNILA W+ + GP+GLA D RRV+FNIISGTSMSCPHVSGL
Sbjct: 492 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 551

Query: 548 AALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVDPASALN 607
           AAL+K  HP+WSPAAIRSALMTTAY  YK+G+ + DIATGKPSTPFDHGAGHV P +A N
Sbjct: 552 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATN 611

Query: 608 PGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAVVFDGVL 667
           PGL+YDLT +DYL FLCALNYT  QI S++RR++TCD  K YSV DLNYPSFAV  DGV 
Sbjct: 612 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV- 671

Query: 668 GGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKKSYTVTF 727
                     K+TRT+T+VG  GTY V ++SET  VKISVEP  L+F  ANEKKSYTVTF
Sbjct: 672 -------GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF 731

Query: 728 TAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           T    S+ P+ +  FG IEWSDGKHVVGSP+A SWT
Sbjct: 732 T--VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of HG10016681 vs. ExPASy Swiss-Prot
Match: Q9ZUF6 (Subtilisin-like protease SBT1.8 OS=Arabidopsis thaliana OX=3702 GN=SBT1.8 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 9.8e-234
Identity = 416/763 (54.52%), Postives = 541/763 (70.90%), Query Frame = 0

Query: 2   ANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEM 61
           ++ + I   F F  + + A    KKTYI+ +     PESF  H  WY S L S S    +
Sbjct: 8   SSSITIITTFLFLLLHTTA----KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SL 67

Query: 62  IYAYNNVVHGFSTRLTAEEAQR-LEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 121
           +Y Y    HGFS  L + EA   L +   IL +  +  Y LHTTRTP+FLGL+    ++ 
Sbjct: 68  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHD 127

Query: 122 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 181
             +S + VIIGVLDTG+WPES+SFDDT +  +PS WKGECESG++F +  CN+KLIGAR 
Sbjct: 128 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 187

Query: 182 FSKGYE-ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 241
           FSKG++ A+ G     +ES SPRD DGHGTHT+TTAAGS V NAS  GYA+GTARGMA R
Sbjct: 188 FSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATR 247

Query: 242 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEK 301
           ARVA YKVCW+ GCF SDILAA+++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+
Sbjct: 248 ARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMER 307

Query: 302 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPL 361
           G+ +SCSAGN+GP+  S++N +PW+ TVGAGTLDRDFPA+ +LG+ K  +GVSLY G  +
Sbjct: 308 GVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGM 367

Query: 362 PGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 421
               L  +Y  N  NS + NLC+ G+L    V GKIV CDRGVN RV+KGAVV+ AGG+G
Sbjct: 368 GTKPLELVY--NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLG 427

Query: 422 MVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSP 481
           M++ANT A+GEELVADSHLLPA AVG+K+GD++R+Y+ S+  PT  ++F+GT L ++PSP
Sbjct: 428 MIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSP 487

Query: 482 VVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMS 541
           VVAAFSSRGPN++TP++LKPD+I PGVNILAGWS ++GP+GL  D RR  FNI+SGTSMS
Sbjct: 488 VVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMS 547

Query: 542 CPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 601
           CPH+SGLA L+K AHP+WSP+AI+SALMTTAY        + D A    S P+ HG+GHV
Sbjct: 548 CPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHV 607

Query: 602 DPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFA 661
           DP  AL+PGLVYD++ ++Y+ FLC+L+YT   I ++ +R     SKK      LNYPSF+
Sbjct: 608 DPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFS 667

Query: 662 VVFDGVLGGGGSGSSVVKHTRTLTNVGSPGT-YKVSISSETKLVKISVEPESLSFTGANE 721
           V+F         G  VV++TR +TNVG+  + YKV+++     V ISV+P  LSF    E
Sbjct: 668 VLF--------GGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGE 727

Query: 722 KKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSW 762
           KK YTVTF +  G +    AE FG I WS+ +H V SP+AFSW
Sbjct: 728 KKRYTVTFVSKKGVSMTNKAE-FGSITWSNPQHEVRSPVAFSW 751

BLAST of HG10016681 vs. ExPASy Swiss-Prot
Match: Q9FLI4 (Subtilisin-like protease SBT1.3 OS=Arabidopsis thaliana OX=3702 GN=SBT1.3 PE=2 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 2.6e-226
Identity = 400/765 (52.29%), Postives = 522/765 (68.24%), Query Frame = 0

Query: 13  FFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVS---------DTAEMIY 72
           F    +      KKTY++HM K  MP  + +HL WY S + SV+         +   ++Y
Sbjct: 22  FLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILY 81

Query: 73  AYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDK--NANLYPE 132
            Y    HG + +LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++  +  ++ E
Sbjct: 82  TYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAE 141

Query: 133 SNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 192
             +  +V++GVLDTGIWPES+SF+DTG+ PVP++W+G CE+G  F   NCNRK++GAR F
Sbjct: 142 RVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVF 201

Query: 193 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 252
            +GYEA  G IDE  E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +AR
Sbjct: 202 YRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKAR 261

Query: 253 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 312
           VAAYKVCW GGCFSSDIL+A+++AV D V VLS+SLGGGVS Y +DS++   F AME G+
Sbjct: 262 VAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGV 321

Query: 313 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPL-- 372
            +SCSAGN GP P SL+N SPWITTVGA T+DRDFPA V +G  + F GVSLY+G+ +  
Sbjct: 322 FVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLP 381

Query: 373 PGTLLPFIY-AANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 432
                P +Y   NAS+    + C+ G L    VAGKIV CDRGV PRVQKG VVK AGGI
Sbjct: 382 KNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGI 441

Query: 433 GMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 492
           GMVL NT  NGEELVADSH+LPA AVG+K G +I++Y ++    T ++   GT++GI+PS
Sbjct: 442 GMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPS 501

Query: 493 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 552
           PVVAAFSSRGPN ++ ++LKPD++APGVNILA W+  + PS L+ D RRV FNI+SGTSM
Sbjct: 502 PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSM 561

Query: 553 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 612
           SCPHVSG+AALIK  HPDWSPAAI+SALMTTAY      + + D +   PS+P+DHGAGH
Sbjct: 562 SCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGH 621

Query: 613 VDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARR-DFTCDSKKKYSVNDLNYPS 672
           +DP  A +PGLVYD+   +Y  FLC  + +PSQ+    +  + TC      +  +LNYP+
Sbjct: 622 IDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPA 681

Query: 673 FAVVFDGVLGGGGSGSSVVKHTRTLTNVGSP-GTYKVSISSETKLVKISVEPESLSFTGA 732
            + +F        +    +   RT+TNVG    +YKVS+ S  K   ++V+P++L+FT  
Sbjct: 682 ISALFP-----ENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSK 741

Query: 733 NEKKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSW 762
           ++K SYTVTF        P     FG + W    H V SP+  +W
Sbjct: 742 HQKLSYTVTFRTRFRMKRPE----FGGLVWKSTTHKVRSPVIITW 776

BLAST of HG10016681 vs. ExPASy Swiss-Prot
Match: Q9LVJ1 (Subtilisin-like protease SBT1.4 OS=Arabidopsis thaliana OX=3702 GN=SBT1.4 PE=2 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.9e-224
Identity = 412/768 (53.65%), Postives = 525/768 (68.36%), Query Frame = 0

Query: 9   LLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDT---AEMIYAY 68
           LL CFFS PS +++   ++YIVH+ +   P  F  H +W+ S LRS+  +   A ++Y+Y
Sbjct: 15  LLLCFFS-PSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSY 74

Query: 69  NNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYPESNSV 128
           +  VHGFS RL+  +   L   P +++V+P+   E+HTT TP FLG  +N+ L+  SN  
Sbjct: 75  SRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYG 134

Query: 129 SEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGY 188
            +VI+GVLDTGIWPE  SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY
Sbjct: 135 EDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGY 194

Query: 189 EATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVA 248
                   +  +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+A
Sbjct: 195 LTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIA 254

Query: 249 AYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGVSDYYKDSVATGAFAAMEKGI 308
           AYK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A GAF A   GI
Sbjct: 255 AYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGI 314

Query: 309 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLPG 368
           ++SCSAGN+GP+P + +N +PWI TVGA T+DR+F A    GD K F+G SLY G+ LP 
Sbjct: 315 VVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPD 374

Query: 369 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 428
           + L  +Y+ +  +     LC  G L    V GKIV CDRG N RV+KG+ VK AGG GM+
Sbjct: 375 SQLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMI 434

Query: 429 LANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLG-IEPSPV 488
           LANT  +GEEL ADSHL+PAT VG K+GD IR Y+ +  +PT  I F GT +G   PSP 
Sbjct: 435 LANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPR 494

Query: 489 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 548
           VAAFSSRGPN +TP +LKPD+IAPGVNILAGW+  VGP+ L ID RRV FNIISGTSMSC
Sbjct: 495 VAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSC 554

Query: 549 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 608
           PHVSGLAAL++ AHPDWSPAAI+SAL+TTAY    +G+ I+D+ATGK S  F HGAGHVD
Sbjct: 555 PHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVD 614

Query: 609 PASALNPGLVYDLTVDDYLNFLCALNYT-PSQINSLARRDF--TCDSKKKYSVNDLNYPS 668
           P  ALNPGLVYD+ V +Y+ FLCA+ Y  P  +  L        C++ K  +  DLNYPS
Sbjct: 615 PNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPS 674

Query: 669 FAVVFDGVLGGGGSGSSVVKHTRTLTNVGS--PGTYKVSISSETKLVKISVEPESLSFTG 728
           F+VVF        S   VVK+ R + NVGS     Y+V + S    V+I V P  L+F+ 
Sbjct: 675 FSVVF-------ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSK 734

Query: 729 ANEKKSYTVTFTAAAGSAAPASAEG--FGRIEWSDGKHVVGSPIAFSW 762
                 Y VTF +        S  G  FG IEW+DG+HVV SP+A  W
Sbjct: 735 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of HG10016681 vs. ExPASy Swiss-Prot
Match: Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.5e-218
Identity = 398/772 (51.55%), Postives = 520/772 (67.36%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSV-SDTA 60
           MA   + F L    S  S A++    TYIVH+     P  F  H HWY SSL S+ S   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60

Query: 61  EMIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGL--DKNAN 120
            +I+ Y+ V HGFS RLT+++A +L   P +++V+PE    LHTTR+P+FLGL     A 
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120

Query: 121 LYPESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIG 180
           L  ES+  S+++IGV+DTG+WPE  SFDD GLGPVP  WKG+C +  +F  S CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180

Query: 181 ARFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMA 240
           ARFF  GYEAT G ++E+ E RSPRD DGHGTHTA+ +AG  V  AS  GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240

Query: 241 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAM 300
            +AR+AAYKVCW  GC+ SDILAA + AV D V+V+S+S+GG V  YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300

Query: 301 EKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK 360
           ++GI +S SAGN GP   +++N +PW+TTVGAGT+DRDFPA V LG+ K  SGVS+Y G 
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360

Query: 361 PL-PGTLLPFIYAAN--ASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 420
            L PG + P +Y  +    +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+ 
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420

Query: 421 AGGIGMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLI------SEPNPTVTILF 480
            GG+GM++AN   +GE LVAD H+LPAT+VG   GD IR+Y+       S  +PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480

Query: 481 EGTKLGIEPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRV 540
           +GT+LGI P+PVVA+FS+RGPN  TP++LKPD+IAPG+NILA W   +GPSG+  D+RR 
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540

Query: 541 DFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKP 600
           +FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT   +G+ + D +TG  
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600

Query: 601 STPFDHGAGHVDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKY 660
           S+  D+G+GHV P  A++PGLVYD+T  DY+NFLC  NYT + I ++ RR   CD  ++ 
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660

Query: 661 S-VNDLNYPSFAVVFDGVLGGGGSGSSVVKHTRTLTNVG-SPGTYKVSISSETKLVKISV 720
             V +LNYPSF+VVF       G         RT+TNVG S   Y++ I    +   ++V
Sbjct: 661 GHVGNLNYPSFSVVFQQY----GESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTV 720

Query: 721 EPESLSFTGANEKKSYTVTFTAAAGSAAP-ASAEGFGRIEWSDGKHVVGSPI 758
           EPE LSF    +K S+ V         +P A+    G I WSDGK  V SP+
Sbjct: 721 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767

BLAST of HG10016681 vs. ExPASy TrEMBL
Match: A0A5D3CR08 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004280 PE=3 SV=1)

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 732/762 (96.06%), Postives = 748/762 (98.16%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60
           MANPVW FLL C FSVPSMA   KKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120
           MIYAYNNVVHGFSTRLT EEAQRLEAQPGILAVVPEMRYELHTTR+PQFLGLDKNANLYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 121 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
           ESNSVSEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 480
           VLANT ANGEELVADSHLLPATAVGQKSGDIIRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660
           P SALNPGLVYDLTVDDYLNFLCALNYTPSQINSLAR+DFTCDSKKKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720
           VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFTGANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 721 SYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           SYTVTFT + GSAAP+SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTFT-SNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of HG10016681 vs. ExPASy TrEMBL
Match: A0A1S3B0Y2 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103484826 PE=3 SV=1)

HSP 1 Score: 1468.4 bits (3800), Expect = 0.0e+00
Identity = 732/762 (96.06%), Postives = 748/762 (98.16%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60
           MANPVW FLL C FSVPSMA   KKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSD+AE
Sbjct: 1   MANPVWTFLLLCLFSVPSMAVGDKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120
           MIYAYNNVVHGFSTRLT EEAQRLEAQPGILAVVPEMRYELHTTR+PQFLGLDKNANLYP
Sbjct: 61  MIYAYNNVVHGFSTRLTTEEAQRLEAQPGILAVVPEMRYELHTTRSPQFLGLDKNANLYP 120

Query: 121 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
           ESNSVSEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF
Sbjct: 121 ESNSVSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AA++KAVEDNVNV+SMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAMDKAVEDNVNVMSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 480
           VLANT ANGEELVADSHLLPATAVGQKSGDIIRKYL+S+P PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDIIRKYLVSDPKPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660
           P SALNPGLVYDLTVDDYLNFLCALNYTPSQINSLAR+DFTCDSKKKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720
           VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETK VKISVEPESLSFTGANEKK
Sbjct: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKSVKISVEPESLSFTGANEKK 720

Query: 721 SYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           SYTVTFT + GSAAP+SAEGFGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTFT-SNGSAAPSSAEGFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of HG10016681 vs. ExPASy TrEMBL
Match: A0A0A0KIG4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G493920 PE=3 SV=1)

HSP 1 Score: 1451.8 bits (3757), Expect = 0.0e+00
Identity = 725/762 (95.14%), Postives = 744/762 (97.64%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAE 60
           MANPVW+FLL CFFSVPSMA    KKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSD+AE
Sbjct: 1   MANPVWMFLLLCFFSVPSMAVGD-KKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDSAE 60

Query: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 120
           MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEM YELHTTR+P+FLGLDKNANLYP
Sbjct: 61  MIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMIYELHTTRSPEFLGLDKNANLYP 120

Query: 121 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 180
           ESNSVSEVIIGVLDTGI PESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKL+GARF
Sbjct: 121 ESNSVSEVIIGVLDTGISPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLVGARF 180

Query: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARA 240
           FSKGYEATLGPIDESKESRSPRDDDGHGTHTA+TAAGSVVENASLFGYASGTARGMAARA
Sbjct: 181 FSKGYEATLGPIDESKESRSPRDDDGHGTHTASTAAGSVVENASLFGYASGTARGMAARA 240

Query: 241 RVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300
           RVAAYKVCWAGGCFSSDI+AAI+KAV+DNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG
Sbjct: 241 RVAAYKVCWAGGCFSSDIVAAIDKAVDDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKG 300

Query: 301 ILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLP 360
           IL+SCSAGNAGPSP+SLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK LP
Sbjct: 301 ILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKSLP 360

Query: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGM 420
           GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGIGM
Sbjct: 361 GTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGIGM 420

Query: 421 VLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPV 480
           VLANT ANGEELVADSHLLPATAVGQKSGD IRKYL+S+P+PTVTILFEGTKLGIEPSPV
Sbjct: 421 VLANTAANGEELVADSHLLPATAVGQKSGDTIRKYLVSDPSPTVTILFEGTKLGIEPSPV 480

Query: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540
           VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC
Sbjct: 481 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 540

Query: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600
           PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD
Sbjct: 541 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 600

Query: 601 PASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAV 660
           P SALNPGLVYDLTVDDYLNFLCALNYTPSQINSLAR+DFTCDSKKKYSVNDLNYPSFAV
Sbjct: 601 PVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARKDFTCDSKKKYSVNDLNYPSFAV 660

Query: 661 VFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKK 720
           VF+GVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSI+SETK VKISVEPESLSFTGAN+KK
Sbjct: 661 VFEGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKK 720

Query: 721 SYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           SYTVTFT    SAAP SAE FGRIEWSDGKHVVGSPIAFSWT
Sbjct: 721 SYTVTFTTTTSSAAPTSAEAFGRIEWSDGKHVVGSPIAFSWT 761

BLAST of HG10016681 vs. ExPASy TrEMBL
Match: A0A6J1GEZ1 (subtilisin-like protease SBT1.7 OS=Cucurbita moschata OX=3662 GN=LOC111453585 PE=3 SV=1)

HSP 1 Score: 1427.5 bits (3694), Expect = 0.0e+00
Identity = 712/764 (93.19%), Postives = 737/764 (96.47%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAA--AQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDT 60
           M  PVWIFLLFCFFS P MAA  A +KKTY+VHMAKYQMP++FEHHLHWYD+SL+SVSDT
Sbjct: 1   MVKPVWIFLLFCFFSGPFMAAAVAAQKKTYVVHMAKYQMPQTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANL 120
           A+MIYAY  VVHGFSTRLTAEEAQRLEAQPG+LAVVPEMRYELHTTRTPQFLGLDKNA+L
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAQRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPES S SEVIIGVLDTG+WPESKSFDD+GLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDSGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GD KNFSGVSLYRGK 
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKS 360

Query: 361 LPGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           LPGTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LPGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 480
           GMVLANT ANGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGL ID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLPIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSF 660
           VDP SALNPGLVYDLTVDDYL+FLCALNYT SQINSLARRD+TCDS KKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANE 720
           AVVFDGVLGGG SGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           KKSYTVTFT   GS+AP SAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTTTGSSAPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of HG10016681 vs. ExPASy TrEMBL
Match: A0A6J1IIG3 (subtilisin-like protease SBT1.7 OS=Cucurbita maxima OX=3661 GN=LOC111477791 PE=3 SV=1)

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 712/764 (93.19%), Postives = 736/764 (96.34%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSM--AAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDT 60
           M  PV IFLLFCFFS P M  AAA +KKTY+VHMAKYQMP +FEHHLHWYD+SL+SVSDT
Sbjct: 1   MEKPVRIFLLFCFFSGPFMAAAAAAQKKTYVVHMAKYQMPLTFEHHLHWYDASLKSVSDT 60

Query: 61  AEMIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANL 120
           A+MIYAY  VVHGFSTRLTAEEA+RLEAQPG+LAVVPEMRYELHTTRTPQFLGLDKNA+L
Sbjct: 61  ADMIYAYKTVVHGFSTRLTAEEAKRLEAQPGVLAVVPEMRYELHTTRTPQFLGLDKNADL 120

Query: 121 YPESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180
           YPES S SEVIIGVLDTG+WPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA
Sbjct: 121 YPESTSTSEVIIGVLDTGVWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGA 180

Query: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240
           RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA
Sbjct: 181 RFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAA 240

Query: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAME 300
           RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGG+SDYYKDSVA+GAFAAME
Sbjct: 241 RARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGISDYYKDSVASGAFAAME 300

Query: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKP 360
           KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVS+GD KNFSGVSLYRGKP
Sbjct: 301 KGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSIGDGKNFSGVSLYRGKP 360

Query: 361 LPGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 420
           L GTLLPFIYAANASNS NGNLCMTGTLIPEKVAGK+VFCDRGVNPRVQKGAVVKAAGGI
Sbjct: 361 LSGTLLPFIYAANASNSANGNLCMTGTLIPEKVAGKVVFCDRGVNPRVQKGAVVKAAGGI 420

Query: 421 GMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 480
           GMVLANT ANGEELVADSHLLPA+AVGQKSGDIIRKYLIS+P PTVTILFEGTKLGIEPS
Sbjct: 421 GMVLANTAANGEELVADSHLLPASAVGQKSGDIIRKYLISDPKPTVTILFEGTKLGIEPS 480

Query: 481 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 540
           PVVAAFSSRGPNSITPQ+LKPDIIAPGVNILAGWSK+VGPSGLAID+RRVDFNIISGTSM
Sbjct: 481 PVVAAFSSRGPNSITPQVLKPDIIAPGVNILAGWSKAVGPSGLAIDERRVDFNIISGTSM 540

Query: 541 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600
           SCPHVSGLAALIKGAH DWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH
Sbjct: 541 SCPHVSGLAALIKGAHLDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 600

Query: 601 VDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSF 660
           VDP SALNPGLVYDLTVDDYL+FLCALNYT SQINSLARRD+TCDS KKYSVN+LNYPSF
Sbjct: 601 VDPVSALNPGLVYDLTVDDYLDFLCALNYTSSQINSLARRDYTCDSGKKYSVNNLNYPSF 660

Query: 661 AVVFDGVLGGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANE 720
           AVVFDGVLGGG SGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFT ANE
Sbjct: 661 AVVFDGVLGGGSSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTKANE 720

Query: 721 KKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           KKSYTVTFT A GS+ P SAEGFGRIEW+DGKHVVGSPIAFSWT
Sbjct: 721 KKSYTVTFTTATGSSVPPSAEGFGRIEWTDGKHVVGSPIAFSWT 764

BLAST of HG10016681 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 1114.0 bits (2880), Expect = 0.0e+00
Identity = 545/756 (72.09%), Postives = 633/756 (83.73%), Query Frame = 0

Query: 8   FLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEMIYAYNN 67
           FLL C       +++  + TYIVHMAK QMP SF+ H +WYDSSLRS+SD+AE++Y Y N
Sbjct: 12  FLLLCLGFCHVSSSSSDQGTYIVHMAKSQMPSSFDLHSNWYDSSLRSISDSAELLYTYEN 71

Query: 68  VVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKN-ANLYPESNSVS 127
            +HGFSTRLT EEA  L  QPG+++V+PE RYELHTTRTP FLGLD++ A+L+PE+ S S
Sbjct: 72  AIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYS 131

Query: 128 EVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGYE 187
           +V++GVLDTG+WPESKS+ D G GP+PSSWKG CE+GTNF+AS CNRKLIGARFF++GYE
Sbjct: 132 DVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYE 191

Query: 188 ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVAAYK 247
           +T+GPIDESKESRSPRDDDGHGTHT++TAAGSVVE ASL GYASGTARGMA RARVA YK
Sbjct: 192 STMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYK 251

Query: 248 VCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGILISCS 307
           VCW GGCFSSDILAAI+KA+ DNVNVLSMSLGGG+SDYY+D VA GAFAAME+GIL+SCS
Sbjct: 252 VCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCS 311

Query: 308 AGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLPGTLLPF 367
           AGNAGPS  SLSN +PWITTVGAGTLDRDFPA   LG+ KNF+GVSL++G+ LP  LLPF
Sbjct: 312 AGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPF 371

Query: 368 IYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMVLANTP 427
           IYA NASN+ NGNLCMTGTLIPEKV GKIV CDRG+N RVQKG VVKAAGG+GM+LANT 
Sbjct: 372 IYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTA 431

Query: 428 ANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSPVVAAFSS 487
           ANGEELVAD+HLLPAT VG+K+GDIIR Y+ ++PNPT +I   GT +G++PSPVVAAFSS
Sbjct: 432 ANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSS 491

Query: 488 RGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSCPHVSGL 547
           RGPNSITP +LKPD+IAPGVNILA W+ + GP+GLA D RRV+FNIISGTSMSCPHVSGL
Sbjct: 492 RGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGL 551

Query: 548 AALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVDPASALN 607
           AAL+K  HP+WSPAAIRSALMTTAY  YK+G+ + DIATGKPSTPFDHGAGHV P +A N
Sbjct: 552 AALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATN 611

Query: 608 PGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFAVVFDGVL 667
           PGL+YDLT +DYL FLCALNYT  QI S++RR++TCD  K YSV DLNYPSFAV  DGV 
Sbjct: 612 PGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGV- 671

Query: 668 GGGGSGSSVVKHTRTLTNVGSPGTYKVSISSETKLVKISVEPESLSFTGANEKKSYTVTF 727
                     K+TRT+T+VG  GTY V ++SET  VKISVEP  L+F  ANEKKSYTVTF
Sbjct: 672 -------GAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTF 731

Query: 728 TAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSWT 763
           T    S+ P+ +  FG IEWSDGKHVVGSP+A SWT
Sbjct: 732 T--VDSSKPSGSNSFGSIEWSDGKHVVGSPVAISWT 757

BLAST of HG10016681 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 811.2 bits (2094), Expect = 7.0e-235
Identity = 416/763 (54.52%), Postives = 541/763 (70.90%), Query Frame = 0

Query: 2   ANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDTAEM 61
           ++ + I   F F  + + A    KKTYI+ +     PESF  H  WY S L S S    +
Sbjct: 8   SSSITIITTFLFLLLHTTA----KKTYIIRVNHSDKPESFLTHHDWYTSQLNSES---SL 67

Query: 62  IYAYNNVVHGFSTRLTAEEAQR-LEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYP 121
           +Y Y    HGFS  L + EA   L +   IL +  +  Y LHTTRTP+FLGL+    ++ 
Sbjct: 68  LYTYTTSFHGFSAYLDSTEADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHD 127

Query: 122 ESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARF 181
             +S + VIIGVLDTG+WPES+SFDDT +  +PS WKGECESG++F +  CN+KLIGAR 
Sbjct: 128 LGSSSNGVIIGVLDTGVWPESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARS 187

Query: 182 FSKGYE-ATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAAR 241
           FSKG++ A+ G     +ES SPRD DGHGTHT+TTAAGS V NAS  GYA+GTARGMA R
Sbjct: 188 FSKGFQMASGGGFSSKRESVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATR 247

Query: 242 ARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEK 301
           ARVA YKVCW+ GCF SDILAA+++A+ D V+VLS+SLGGG + YY+D++A GAF+AME+
Sbjct: 248 ARVATYKVCWSTGCFGSDILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMER 307

Query: 302 GILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPL 361
           G+ +SCSAGN+GP+  S++N +PW+ TVGAGTLDRDFPA+ +LG+ K  +GVSLY G  +
Sbjct: 308 GVFVSCSAGNSGPTRASVANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGM 367

Query: 362 PGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIG 421
               L  +Y  N  NS + NLC+ G+L    V GKIV CDRGVN RV+KGAVV+ AGG+G
Sbjct: 368 GTKPLELVY--NKGNSSSSNLCLPGSLDSSIVRGKIVVCDRGVNARVEKGAVVRDAGGLG 427

Query: 422 MVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPSP 481
           M++ANT A+GEELVADSHLLPA AVG+K+GD++R+Y+ S+  PT  ++F+GT L ++PSP
Sbjct: 428 MIMANTAASGEELVADSHLLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSP 487

Query: 482 VVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMS 541
           VVAAFSSRGPN++TP++LKPD+I PGVNILAGWS ++GP+GL  D RR  FNI+SGTSMS
Sbjct: 488 VVAAFSSRGPNTVTPEILKPDVIGPGVNILAGWSDAIGPTGLDKDSRRTQFNIMSGTSMS 547

Query: 542 CPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHV 601
           CPH+SGLA L+K AHP+WSP+AI+SALMTTAY        + D A    S P+ HG+GHV
Sbjct: 548 CPHISGLAGLLKAAHPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHV 607

Query: 602 DPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKYSVNDLNYPSFA 661
           DP  AL+PGLVYD++ ++Y+ FLC+L+YT   I ++ +R     SKK      LNYPSF+
Sbjct: 608 DPQKALSPGLVYDISTEEYIRFLCSLDYTVDHIVAIVKRPSVNCSKKFSDPGQLNYPSFS 667

Query: 662 VVFDGVLGGGGSGSSVVKHTRTLTNVGSPGT-YKVSISSETKLVKISVEPESLSFTGANE 721
           V+F         G  VV++TR +TNVG+  + YKV+++     V ISV+P  LSF    E
Sbjct: 668 VLF--------GGKRVVRYTREVTNVGAASSVYKVTVNGAPS-VGISVKPSKLSFKSVGE 727

Query: 722 KKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSW 762
           KK YTVTF +  G +    AE FG I WS+ +H V SP+AFSW
Sbjct: 728 KKRYTVTFVSKKGVSMTNKAE-FGSITWSNPQHEVRSPVAFSW 751

BLAST of HG10016681 vs. TAIR 10
Match: AT5G51750.1 (subtilase 1.3 )

HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-227
Identity = 400/765 (52.29%), Postives = 522/765 (68.24%), Query Frame = 0

Query: 13  FFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVS---------DTAEMIY 72
           F    +      KKTY++HM K  MP  + +HL WY S + SV+         +   ++Y
Sbjct: 22  FLQAETTTQISTKKTYVIHMDKSAMPLPYTNHLQWYSSKINSVTQHKSQEEEGNNNRILY 81

Query: 73  AYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDK--NANLYPE 132
            Y    HG + +LT EEA+RLE + G++AV+PE RYELHTTR+P FLGL++  +  ++ E
Sbjct: 82  TYQTAFHGLAAQLTQEEAERLEEEDGVVAVIPETRYELHTTRSPTFLGLERQESERVWAE 141

Query: 133 SNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFF 192
             +  +V++GVLDTGIWPES+SF+DTG+ PVP++W+G CE+G  F   NCNRK++GAR F
Sbjct: 142 RVTDHDVVVGVLDTGIWPESESFNDTGMSPVPATWRGACETGKRFLKRNCNRKIVGARVF 201

Query: 193 SKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARAR 252
            +GYEA  G IDE  E +SPRD DGHGTHTA T AGS V+ A+LFG+A GTARGMA +AR
Sbjct: 202 YRGYEAATGKIDEELEYKSPRDRDGHGTHTAATVAGSPVKGANLFGFAYGTARGMAQKAR 261

Query: 253 VAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAMEKGI 312
           VAAYKVCW GGCFSSDIL+A+++AV D V VLS+SLGGGVS Y +DS++   F AME G+
Sbjct: 262 VAAYKVCWVGGCFSSDILSAVDQAVADGVQVLSISLGGGVSTYSRDSLSIATFGAMEMGV 321

Query: 313 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPL-- 372
            +SCSAGN GP P SL+N SPWITTVGA T+DRDFPA V +G  + F GVSLY+G+ +  
Sbjct: 322 FVSCSAGNGGPDPISLTNVSPWITTVGASTMDRDFPATVKIGTMRTFKGVSLYKGRTVLP 381

Query: 373 PGTLLPFIY-AANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGI 432
                P +Y   NAS+    + C+ G L    VAGKIV CDRGV PRVQKG VVK AGGI
Sbjct: 382 KNKQYPLVYLGRNASSPDPTSFCLDGALDRRHVAGKIVICDRGVTPRVQKGQVVKRAGGI 441

Query: 433 GMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLGIEPS 492
           GMVL NT  NGEELVADSH+LPA AVG+K G +I++Y ++    T ++   GT++GI+PS
Sbjct: 442 GMVLTNTATNGEELVADSHMLPAVAVGEKEGKLIKQYAMTSKKATASLEILGTRIGIKPS 501

Query: 493 PVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSM 552
           PVVAAFSSRGPN ++ ++LKPD++APGVNILA W+  + PS L+ D RRV FNI+SGTSM
Sbjct: 502 PVVAAFSSRGPNFLSLEILKPDLLAPGVNILAAWTGDMAPSSLSSDPRRVKFNILSGTSM 561

Query: 553 SCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGH 612
           SCPHVSG+AALIK  HPDWSPAAI+SALMTTAY      + + D +   PS+P+DHGAGH
Sbjct: 562 SCPHVSGVAALIKSRHPDWSPAAIKSALMTTAYVHDNMFKPLTDASGAAPSSPYDHGAGH 621

Query: 613 VDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARR-DFTCDSKKKYSVNDLNYPS 672
           +DP  A +PGLVYD+   +Y  FLC  + +PSQ+    +  + TC      +  +LNYP+
Sbjct: 622 IDPLRATDPGLVYDIGPQEYFEFLCTQDLSPSQLKVFTKHSNRTCKHTLAKNPGNLNYPA 681

Query: 673 FAVVFDGVLGGGGSGSSVVKHTRTLTNVGSP-GTYKVSISSETKLVKISVEPESLSFTGA 732
            + +F        +    +   RT+TNVG    +YKVS+ S  K   ++V+P++L+FT  
Sbjct: 682 ISALFP-----ENTHVKAMTLRRTVTNVGPHISSYKVSV-SPFKGASVTVQPKTLNFTSK 741

Query: 733 NEKKSYTVTFTAAAGSAAPASAEGFGRIEWSDGKHVVGSPIAFSW 762
           ++K SYTVTF        P     FG + W    H V SP+  +W
Sbjct: 742 HQKLSYTVTFRTRFRMKRPE----FGGLVWKSTTHKVRSPVIITW 776

BLAST of HG10016681 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 780.4 bits (2014), Expect = 1.3e-225
Identity = 412/768 (53.65%), Postives = 525/768 (68.36%), Query Frame = 0

Query: 9   LLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSVSDT---AEMIYAY 68
           LL CFFS PS +++   ++YIVH+ +   P  F  H +W+ S LRS+  +   A ++Y+Y
Sbjct: 15  LLLCFFS-PSSSSSDGLESYIVHVQRSHKPSLFSSHNNWHVSLLRSLPSSPQPATLLYSY 74

Query: 69  NNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGLDKNANLYPESNSV 128
           +  VHGFS RL+  +   L   P +++V+P+   E+HTT TP FLG  +N+ L+  SN  
Sbjct: 75  SRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYG 134

Query: 129 SEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIGARFFSKGY 188
            +VI+GVLDTGIWPE  SF D+GLGP+PS+WKGECE G +F AS+CNRKLIGAR F +GY
Sbjct: 135 EDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGY 194

Query: 189 EATLGPIDE--SKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMAARARVA 248
                   +  +KESRSPRD +GHGTHTA+TAAGSVV NASL+ YA GTA GMA++AR+A
Sbjct: 195 LTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIA 254

Query: 249 AYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLG--GGVSDYYKDSVATGAFAAMEKGI 308
           AYK+CW GGC+ SDILAA+++AV D V+V+S+S+G  G   +Y+ DS+A GAF A   GI
Sbjct: 255 AYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGI 314

Query: 309 LISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGKPLPG 368
           ++SCSAGN+GP+P + +N +PWI TVGA T+DR+F A    GD K F+G SLY G+ LP 
Sbjct: 315 VVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPD 374

Query: 369 TLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKAAGGIGMV 428
           + L  +Y+ +  +     LC  G L    V GKIV CDRG N RV+KG+ VK AGG GM+
Sbjct: 375 SQLSLVYSGDCGS----RLCYPGKLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMI 434

Query: 429 LANTPANGEELVADSHLLPATAVGQKSGDIIRKYLISEPNPTVTILFEGTKLG-IEPSPV 488
           LANT  +GEEL ADSHL+PAT VG K+GD IR Y+ +  +PT  I F GT +G   PSP 
Sbjct: 435 LANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPR 494

Query: 489 VAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRVDFNIISGTSMSC 548
           VAAFSSRGPN +TP +LKPD+IAPGVNILAGW+  VGP+ L ID RRV FNIISGTSMSC
Sbjct: 495 VAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSC 554

Query: 549 PHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKPSTPFDHGAGHVD 608
           PHVSGLAAL++ AHPDWSPAAI+SAL+TTAY    +G+ I+D+ATGK S  F HGAGHVD
Sbjct: 555 PHVSGLAALLRKAHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVD 614

Query: 609 PASALNPGLVYDLTVDDYLNFLCALNYT-PSQINSLARRDF--TCDSKKKYSVNDLNYPS 668
           P  ALNPGLVYD+ V +Y+ FLCA+ Y  P  +  L        C++ K  +  DLNYPS
Sbjct: 615 PNKALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPS 674

Query: 669 FAVVFDGVLGGGGSGSSVVKHTRTLTNVGS--PGTYKVSISSETKLVKISVEPESLSFTG 728
           F+VVF        S   VVK+ R + NVGS     Y+V + S    V+I V P  L+F+ 
Sbjct: 675 FSVVF-------ASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPAN-VEIDVSPSKLAFSK 734

Query: 729 ANEKKSYTVTFTAAAGSAAPASAEG--FGRIEWSDGKHVVGSPIAFSW 762
                 Y VTF +        S  G  FG IEW+DG+HVV SP+A  W
Sbjct: 735 EKSVLEYEVTFKSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQW 769

BLAST of HG10016681 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 760.8 bits (1963), Expect = 1.1e-219
Identity = 398/772 (51.55%), Postives = 520/772 (67.36%), Query Frame = 0

Query: 1   MANPVWIFLLFCFFSVPSMAAAQKKKTYIVHMAKYQMPESFEHHLHWYDSSLRSV-SDTA 60
           MA   + F L    S  S A++    TYIVH+     P  F  H HWY SSL S+ S   
Sbjct: 1   MAFFFYFFFLLTLSSPSSSASSSNSLTYIVHVDHEAKPSIFPTHFHWYTSSLASLTSSPP 60

Query: 61  EMIYAYNNVVHGFSTRLTAEEAQRLEAQPGILAVVPEMRYELHTTRTPQFLGL--DKNAN 120
            +I+ Y+ V HGFS RLT+++A +L   P +++V+PE    LHTTR+P+FLGL     A 
Sbjct: 61  SIIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAG 120

Query: 121 LYPESNSVSEVIIGVLDTGIWPESKSFDDTGLGPVPSSWKGECESGTNFSASNCNRKLIG 180
           L  ES+  S+++IGV+DTG+WPE  SFDD GLGPVP  WKG+C +  +F  S CNRKL+G
Sbjct: 121 LLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVG 180

Query: 181 ARFFSKGYEATLGPIDESKESRSPRDDDGHGTHTATTAAGSVVENASLFGYASGTARGMA 240
           ARFF  GYEAT G ++E+ E RSPRD DGHGTHTA+ +AG  V  AS  GYA G A GMA
Sbjct: 181 ARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMA 240

Query: 241 ARARVAAYKVCWAGGCFSSDILAAIEKAVEDNVNVLSMSLGGGVSDYYKDSVATGAFAAM 300
            +AR+AAYKVCW  GC+ SDILAA + AV D V+V+S+S+GG V  YY D++A GAF A+
Sbjct: 241 PKARLAAYKVCWNSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAI 300

Query: 301 EKGILISCSAGNAGPSPYSLSNTSPWITTVGAGTLDRDFPAYVSLGDAKNFSGVSLYRGK 360
           ++GI +S SAGN GP   +++N +PW+TTVGAGT+DRDFPA V LG+ K  SGVS+Y G 
Sbjct: 301 DRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGP 360

Query: 361 PL-PGTLLPFIYAAN--ASNSGNGNLCMTGTLIPEKVAGKIVFCDRGVNPRVQKGAVVKA 420
            L PG + P +Y  +    +  + +LC+ G+L P  V GKIV CDRG+N R  KG +V+ 
Sbjct: 361 GLDPGRMYPLVYGGSLLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSRATKGEIVRK 420

Query: 421 AGGIGMVLANTPANGEELVADSHLLPATAVGQKSGDIIRKYLI------SEPNPTVTILF 480
            GG+GM++AN   +GE LVAD H+LPAT+VG   GD IR+Y+       S  +PT TI+F
Sbjct: 421 NGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVF 480

Query: 481 EGTKLGIEPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSVGPSGLAIDDRRV 540
           +GT+LGI P+PVVA+FS+RGPN  TP++LKPD+IAPG+NILA W   +GPSG+  D+RR 
Sbjct: 481 KGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRT 540

Query: 541 DFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKNGQKIQDIATGKP 600
           +FNI+SGTSM+CPHVSGLAAL+K AHPDWSPAAIRSAL+TTAYT   +G+ + D +TG  
Sbjct: 541 EFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNT 600

Query: 601 STPFDHGAGHVDPASALNPGLVYDLTVDDYLNFLCALNYTPSQINSLARRDFTCDSKKKY 660
           S+  D+G+GHV P  A++PGLVYD+T  DY+NFLC  NYT + I ++ RR   CD  ++ 
Sbjct: 601 SSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRA 660

Query: 661 S-VNDLNYPSFAVVFDGVLGGGGSGSSVVKHTRTLTNVG-SPGTYKVSISSETKLVKISV 720
             V +LNYPSF+VVF       G         RT+TNVG S   Y++ I    +   ++V
Sbjct: 661 GHVGNLNYPSFSVVFQQY----GESKMSTHFIRTVTNVGDSDSVYEIKI-RPPRGTTVTV 720

Query: 721 EPESLSFTGANEKKSYTVTFTAAAGSAAP-ASAEGFGRIEWSDGKHVVGSPI 758
           EPE LSF    +K S+ V         +P A+    G I WSDGK  V SP+
Sbjct: 721 EPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPL 767