HG10016552 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10016552 vs. NCBI nr
Match: XP_038882187.1 (RNA cytosine C(5)-methyltransferase NSUN2-like [Benincasa hispida])

HSP 1 Score: 1571.6 bits (4068), Expect = 0.0e+00
Identity = 792/849 (93.29%), Postives = 816/849 (96.11%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWEPFESQSPAF 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+ DPNSANDNKD  GNGNGNPWEPFESQSPAF
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRSDPNSANDNKDGNGNGNGNPWEPFESQSPAF 60

Query: 61  NEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTE 120
           NEYYKEQGIV+ EEW+ FIEVLRKPLPAAFRINSSGQFC+EIRAQL+NDF KSLQAEVTE
Sbjct: 61  NEYYKEQGIVSEEEWNTFIEVLRKPLPAAFRINSSGQFCDEIRAQLQNDFMKSLQAEVTE 120

Query: 121 GDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180
           GDEA+AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP
Sbjct: 121 GDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240
           PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIH+SSKPGDLPDGLVVANDLDVQRCNLLIHQ
Sbjct: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHKSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDG 300
           TKRMCTANLIVTNHEAQHFPGCRAHT+FF+ASAAGSEVKP NTQLTFDRVLCDVPCSGDG
Sbjct: 241 TKRMCTANLIVTNHEAQHFPGCRAHTSFFNASAAGSEVKPHNTQLTFDRVLCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL
Sbjct: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360

Query: 361 LRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMFPSG 420
           LR+SGGSLELVDVSNELPQLV RPGLKKWKVRDRG W ASYKE +EGRQSVAIPSMFPSG
Sbjct: 361 LRKSGGSLELVDVSNELPQLVCRPGLKKWKVRDRGVWLASYKEIIEGRQSVAIPSMFPSG 420

Query: 421 RGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQ 480
           RGRKNQAD+NHNSEL GNHLDGSIGSS DVSE  MDPV EFDEVCEFPIERC+RIVPHDQ
Sbjct: 421 RGRKNQADNNHNSELGGNHLDGSIGSSVDVSEPIMDPVLEFDEVCEFPIERCIRIVPHDQ 480

Query: 481 NSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDESPSE 540
           NSGAFFIAVLRKVAPLPAA+EKQNSKSA+DRSRCKLPQTIADGESV+VDAAVGMDESPSE
Sbjct: 481 NSGAFFIAVLRKVAPLPAAVEKQNSKSADDRSRCKLPQTIADGESVNVDAAVGMDESPSE 540

Query: 541 VGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVD-NEV 600
           VGID NNQ D NSADNKPACE PVQDIKDADGLDT  VDG  KQFSEADL AKLVD NEV
Sbjct: 541 VGIDPNNQEDFNSADNKPACELPVQDIKDADGLDTNAVDGRGKQFSEADLAAKLVDNNEV 600

Query: 601 EENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVINSI 660
           +ENNLEV AIKDNSEESKQPDD EMDPKKSSVKRKLQIQGKW+GVDPVVLFKDETVI+SI
Sbjct: 601 DENNLEVTAIKDNSEESKQPDDGEMDPKKSSVKRKLQIQGKWKGVDPVVLFKDETVIDSI 660

Query: 661 KTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLKMF 720
           KTFYGINESFPLIGHLVTRNSDTNHVKRIYYIS S KDVLELNF+VGQQLKITSIGLKMF
Sbjct: 661 KTFYGINESFPLIGHLVTRNSDTNHVKRIYYISNSVKDVLELNFAVGQQLKITSIGLKMF 720

Query: 721 ERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAF 780
           ERQSSREG+SAPC FRISSEGLPVILP+ITKQILTMSPVDFKHLLQYKSIKYADFVDAAF
Sbjct: 721 ERQSSREGSSAPCSFRISSEGLPVILPHITKQILTMSPVDFKHLLQYKSIKYADFVDAAF 780

Query: 781 GEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELLER 840
           GEKASNLMLGCCVIVLGTGSNTSSDVIKVD+STIAIGCWKGRASLSVMVTAIDCQELL R
Sbjct: 781 GEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRASLSVMVTAIDCQELLGR 840

Query: 841 LSNRLENDL 848
           LS RLEN+L
Sbjct: 841 LSKRLENNL 849

BLAST of HG10016552 vs. NCBI nr
Match: XP_011657964.1 (RNA cytosine C(5)-methyltransferase NSUN2 [Cucumis sativus] >KGN48733.1 hypothetical protein Csa_003095 [Cucumis sativus])

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 766/849 (90.22%), Postives = 794/849 (93.52%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDN-KDAKGNGNGNPWEPFESQSPA 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSANDN KD  GNGNG PWEPFESQSPA
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANDNDKDGNGNGNGKPWEPFESQSPA 60

Query: 61  FNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVT 120
           FNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAEVT
Sbjct: 61  FNEYYKEQFIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAEVT 120

Query: 121 EGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 180
           EGDEA AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV
Sbjct: 121 EGDEAVAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 180

Query: 181 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIH 240
           PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLLIH
Sbjct: 181 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLLIH 240

Query: 241 QTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGD 300
           QTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASA+GSEVKP NTQLTFDRVLCDVPCSGD
Sbjct: 241 QTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASASGSEVKPHNTQLTFDRVLCDVPCSGD 300

Query: 301 GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 360
           GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE
Sbjct: 301 GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 360

Query: 361 LLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMFPS 420
           LLRRSGGSLEL+DVSNELPQLVRRPGLKKW+VRDRG W ASYKE +EGRQSVAIPSMFPS
Sbjct: 361 LLRRSGGSLELIDVSNELPQLVRRPGLKKWRVRDRGVWLASYKEIIEGRQSVAIPSMFPS 420

Query: 421 GRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHD 480
           GRGRKNQAD+N NSEL  NHLDGSIGSS DVSE  MDPV  FDEVCEFPIERCMRIVPHD
Sbjct: 421 GRGRKNQADNNDNSELGVNHLDGSIGSSVDVSEPIMDPVDGFDEVCEFPIERCMRIVPHD 480

Query: 481 QNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDESPS 540
           QNSGAFFIAVLRK+APLPA  EKQ SKS+NDRSRCKLPQTIADGESV+VDAAVGMDES S
Sbjct: 481 QNSGAFFIAVLRKIAPLPATFEKQTSKSSNDRSRCKLPQTIADGESVEVDAAVGMDESSS 540

Query: 541 EVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDNEV 600
           E GI SNNQG+ +SAD KPACE PV D KDADGLDT          +  D EAKLVDNEV
Sbjct: 541 EAGIISNNQGEPSSAD-KPACELPVHDNKDADGLDT----------NAGDSEAKLVDNEV 600

Query: 601 EENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVINSI 660
           +ENNLEVAAIK  SEESKQPDD E+DP KSS KRKLQIQGKW+GVDPVVLF DETVI+S+
Sbjct: 601 DENNLEVAAIKIYSEESKQPDDGEVDPTKSSAKRKLQIQGKWKGVDPVVLFNDETVIDSV 660

Query: 661 KTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLKMF 720
           KTFYGI+ESFPL+GHLVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLKMF
Sbjct: 661 KTFYGIDESFPLVGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMF 720

Query: 721 ERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAF 780
           ERQSSREG+SAPCLFRISSEGLPVILPYITKQILT+SPVDFKHLLQYKS+KYADFVD+AF
Sbjct: 721 ERQSSREGSSAPCLFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSVKYADFVDSAF 780

Query: 781 GEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELLER 840
           GEKASNLMLGCCV+VLGTG+NTSSD IKVD STIAIGCWKGRASLSVMVTAIDCQELLER
Sbjct: 781 GEKASNLMLGCCVVVLGTGTNTSSDDIKVDSSTIAIGCWKGRASLSVMVTAIDCQELLER 838

Query: 841 LSNRLENDL 848
           LSNRLENDL
Sbjct: 841 LSNRLENDL 838

BLAST of HG10016552 vs. NCBI nr
Match: XP_008440576.1 (PREDICTED: tRNA (cytosine(34)-C(5))-methyltransferase [Cucumis melo] >KAA0036318.1 tRNA (cytosine(34)-C(5))-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 1495.3 bits (3870), Expect = 0.0e+00
Identity = 759/851 (89.19%), Postives = 793/851 (93.18%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNK---DAKGNGNGNPWEPFESQS 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSAN N    +  GNGNGNPWEPFESQS
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANHNDKDGNGNGNGNGNPWEPFESQS 60

Query: 61  PAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAE 120
           PAFNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAE
Sbjct: 61  PAFNEYYKEQSIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAE 120

Query: 121 VTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180
           VTE DEA+AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS
Sbjct: 121 VTESDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180

Query: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLL 240
           MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLL
Sbjct: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLL 240

Query: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCS 300
           IHQTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASAAGSEVKP NTQLTFDRVLCDVPCS
Sbjct: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASAAGSEVKPHNTQLTFDRVLCDVPCS 300

Query: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360
           GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV
Sbjct: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360

Query: 361 AELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMF 420
           AELLR+SGGSLEL+DVSNELPQLVRRPGLKKW+V DRG W ASYKE +EGRQSVAIPSMF
Sbjct: 361 AELLRKSGGSLELIDVSNELPQLVRRPGLKKWRVLDRGVWLASYKEIIEGRQSVAIPSMF 420

Query: 421 PSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVP 480
           PSGRGRKNQAD+N NSEL GNHLDGSIGSS DVSE T+DPV  FDEVCEFPIERCMRIVP
Sbjct: 421 PSGRGRKNQADNNDNSELGGNHLDGSIGSSVDVSEPTVDPVDGFDEVCEFPIERCMRIVP 480

Query: 481 HDQNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           HDQNSGAFFIAVLRK+APLPA  EKQ SKSANDRSRCKLPQTIADGE+VDVDAAVGMDES
Sbjct: 481 HDQNSGAFFIAVLRKIAPLPATFEKQTSKSANDRSRCKLPQTIADGENVDVDAAVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           P+E GI SNNQG+S+SAD KPACE P+QD KDA+GL+T          +  D EAKLVDN
Sbjct: 541 PTEAGIISNNQGESSSAD-KPACELPIQDNKDAEGLNT----------NAGDSEAKLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV+E     AAIK  SEESKQPDD E+DPKKS+ KRKLQIQGKW+GVDPVVLFKDETVI+
Sbjct: 601 EVDEK----AAIKIYSEESKQPDDGEVDPKKSAAKRKLQIQGKWKGVDPVVLFKDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGI+ESFPL+G LVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLK
Sbjct: 661 SIKTFYGIDESFPLVGQLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+SAPC FRISSEGLPVILPYITKQILT+SPVDFKHLLQYKSIKY+DFVD+
Sbjct: 721 MFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYSDFVDS 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTG+NTSSDVIKVD STIAIGCWKGRASLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGTNTSSDVIKVDSSTIAIGCWKGRASLSVMVTAIDCQELL 836

Query: 841 ERLSNRLENDL 848
           ERLSNRLEN+L
Sbjct: 841 ERLSNRLENNL 836

BLAST of HG10016552 vs. NCBI nr
Match: TYK12712.1 (tRNA (cytosine(34)-C(5))-methyltransferase [Cucumis melo var. makuwa])

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 758/851 (89.07%), Postives = 792/851 (93.07%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNK---DAKGNGNGNPWEPFESQS 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSAN N    +  GNGNGNPWEPFESQS
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANHNDKDGNGNGNGNGNPWEPFESQS 60

Query: 61  PAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAE 120
           PAFNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAE
Sbjct: 61  PAFNEYYKEQSIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAE 120

Query: 121 VTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180
           VTE DEA+AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS
Sbjct: 121 VTESDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180

Query: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLL 240
           MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLL
Sbjct: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLL 240

Query: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCS 300
           IHQTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASAAGSEVKP NTQLTFDRVLCDVPCS
Sbjct: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASAAGSEVKPHNTQLTFDRVLCDVPCS 300

Query: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360
           GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV
Sbjct: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360

Query: 361 AELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMF 420
           AELLR+SGGSLEL+DVSNELPQLVRRPGL KW+V DRG W ASYKE +EGRQSVAIPSMF
Sbjct: 361 AELLRKSGGSLELIDVSNELPQLVRRPGLNKWRVLDRGVWLASYKEIIEGRQSVAIPSMF 420

Query: 421 PSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVP 480
           PSGRGRKNQAD+N NSEL GNHLDGSIGSS DVSE T+DPV  FDEVCEFPIERCMRIVP
Sbjct: 421 PSGRGRKNQADNNDNSELGGNHLDGSIGSSVDVSEPTVDPVDGFDEVCEFPIERCMRIVP 480

Query: 481 HDQNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           HDQNSGAFFIAVLRK+APLPA  EKQ SKSANDRSRCKLPQTIADGE+VDVDAAVGMDES
Sbjct: 481 HDQNSGAFFIAVLRKIAPLPATFEKQTSKSANDRSRCKLPQTIADGENVDVDAAVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           P+E GI SNNQG+S+SAD KPACE P+QD KDA+GL+T          +  D EAKLVDN
Sbjct: 541 PTEAGIISNNQGESSSAD-KPACELPIQDNKDAEGLNT----------NAGDSEAKLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV+E     AAIK  SEESKQPDD E+DPKKS+ KRKLQIQGKW+GVDPVVLFKDETVI+
Sbjct: 601 EVDEK----AAIKIYSEESKQPDDGEVDPKKSAAKRKLQIQGKWKGVDPVVLFKDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGI+ESFPL+G LVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLK
Sbjct: 661 SIKTFYGIDESFPLVGQLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+SAPC FRISSEGLPVILPYITKQILT+SPVDFKHLLQYKSIKY+DFVD+
Sbjct: 721 MFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYSDFVDS 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTG+NTSSDVIKVD STIAIGCWKGRASLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGTNTSSDVIKVDSSTIAIGCWKGRASLSVMVTAIDCQELL 836

Query: 841 ERLSNRLENDL 848
           ERLSNRLEN+L
Sbjct: 841 ERLSNRLENNL 836

BLAST of HG10016552 vs. NCBI nr
Match: KAG6604044.1 (RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034210.1 tRNA (cytosine(34)-C(5))-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 749/851 (88.01%), Postives = 789/851 (92.71%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWEPFESQSPAF 60
           MGGKGRGGRARTQR HFR++RENVWKR KPD NSAND KD    GN N W PF+SQS AF
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKD----GNSNHWGPFQSQSHAF 60

Query: 61  NEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTE 120
           NEYYKEQGIVT+EEWD FIEVLRK LPAAFRINSSGQFC EIRAQL+NDF KSLQ+EVT+
Sbjct: 61  NEYYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTD 120

Query: 121 GDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180
           GDEA AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP
Sbjct: 121 GDEAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240
           PLFLDVH NHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQ
Sbjct: 181 PLFLDVHRNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDG 300
           TKRMCT+NLIVTNHEAQHFPGCRAHTNFF+ASAAG E+   NTQLTFDRVLCDVPCSGDG
Sbjct: 241 TKRMCTSNLIVTNHEAQHFPGCRAHTNFFNASAAGIELNHHNTQLTFDRVLCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRKAPDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL
Sbjct: 301 TLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360

Query: 361 LRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKEV-EGRQSVAIPSMFPSG 420
           LR+S GSLELVDVSNELPQLVRRPGLKKWKVRDRG W ASYK+V + RQSVAIPSMFPSG
Sbjct: 361 LRKSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSG 420

Query: 421 RGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQ 480
           RGRKNQAD +HNSELRGNHLDGSIGSS DVSE TMDPVHEFDEVCEFPIERCMRIVPHDQ
Sbjct: 421 RGRKNQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQ 480

Query: 481 NSGAFFIAVLRKVAPLPAAIEK---QNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           NSGAFFIAVLRKVAPLPAA EK   QN K  ND+SRCKLPQTI +G +VDV A+VGMDES
Sbjct: 481 NSGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           PSEVGIDSNNQGD NSADNKPACE PVQDI+  +GLD+   DG+DKQFSEADLE  LVDN
Sbjct: 541 PSEVGIDSNNQGDFNSADNKPACEFPVQDIQGTEGLDSNAADGIDKQFSEADLEVNLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV  NNLE+ AI+DN EESKQPDD EMDPKKS+VKRKLQIQGKW+GVDPVVLFKDETVI+
Sbjct: 601 EVSVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKS KDVLELNF+VGQQLKITSIGLK
Sbjct: 661 SIKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFAVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+SAPC FRI+SEGLPVILPYITKQILTMSPVDFKHL+Q+KSIKYADF DA
Sbjct: 721 MFERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADA 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVD+STIAIGCWKGR+SLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELL 840

Query: 841 ERLSNRLENDL 848
           ERLSNRL ND+
Sbjct: 841 ERLSNRLGNDV 847

BLAST of HG10016552 vs. ExPASy Swiss-Prot
Match: Q1HFZ0 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Mus musculus OX=10090 GN=Nsun2 PE=1 SV=2)

HSP 1 Score: 411.8 bits (1057), Expect = 1.9e-113
Identity = 277/805 (34.41%), Postives = 410/805 (50.93%), Query Frame = 0

Query: 30  PDPNSANDNKDAKGNGNGNPWE----PFESQSPAFNEYYKEQGIVTSEEWDAFIEVLRKP 89
           P P    D  D         WE        ++  F  YY+E  IV   EWD F+E LR+P
Sbjct: 14  PQPEGEEDASDGGRKRGQAGWEGGYPEIVKENKLFEHYYQELKIVPEGEWDQFMESLREP 73

Query: 90  LPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTEGDEADAIRPLPWYPDNLAWHSNFSR 149
           LPA  RI       +EI   L+N + K L+    +G + +  +PL WYP+ LAWH+N SR
Sbjct: 74  LPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDGQKVEVPQPLSWYPEELAWHTNLSR 133

Query: 150 MQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQ 209
             LRK+  L +FH+FL  E E GNI+RQEAVSM+PPL L+V P+H +LDMCAAPGSKT Q
Sbjct: 134 KILRKSPLLAKFHQFLVSETESGNISRQEAVSMIPPLLLNVEPHHKILDMCAAPGSKTTQ 193

Query: 210 LLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMCTANLIVTNHEAQHFPGCRAH 269
           L+E++H +      P+G V+AND+D +RC LL+HQ KR+ +  ++V NH+A   P     
Sbjct: 194 LIEMLH-ADMSVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLTV- 253

Query: 270 TNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQV 329
                      +V      L +DR+LCDVPCSGDGT+RK  D+W+KW +     LHGLQ+
Sbjct: 254 -----------DVDGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQL 313

Query: 330 QIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRRSGGSLELVDVSNELPQLVRRPG 389
           +I  RGA  L  GGRMVYSTCS+NPVE+EAV+A LL +S G+LEL DVS ELP L   PG
Sbjct: 314 RIATRGAEQLAEGGRMVYSTCSLNPVEDEAVIAALLEKSEGALELADVSAELPGLKWMPG 373

Query: 390 LKKWKVRDR-GEWSASYKEV-EGRQSVAIPSMFPSGRGRKNQADSNHNSELRGNHLDGSI 449
           + +WKV  R G+W A + EV +GR +   P+MFP     K QA                 
Sbjct: 374 VSQWKVMTRDGQWFADWHEVPQGRHTQIRPTMFPPTDLEKLQA----------------- 433

Query: 450 GSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFFIAVLRKVAPLPAAIEKQN 509
                                   +ERC+RI+PH QN+G FF+AVL K AP+P    K+ 
Sbjct: 434 ----------------------MHLERCLRILPHHQNTGGFFVAVLVKKAPMP--WNKRQ 493

Query: 510 SKSANDRSRCKLPQTIADGESVDVDAAVGMDESPSEVGIDSNNQGDSNSADNKPACEPPV 569
            K  N  +  + P+                    S V     N  D              
Sbjct: 494 PKVQNKSAEAREPRV------------------SSHVAATEGNPSD-------------- 553

Query: 570 QDIKDADGLDTKEVDGMDKQFSEADLEAKLVDNEVEENNLEVAAIKDNSEESKQPDDREM 629
                                 +++LE++++       + E A   +N+E +++ D    
Sbjct: 554 ----------------------QSELESQMITG---AGDSETAHNTENTESNEKKDGVCG 613

Query: 630 DPKKSSVKRKLQIQGKWRGVDPVVLF-KDETVINSIKTFYGINESFPLIGHLVTRNSDTN 689
            P      +K+++ G     DP V   +D+ +   I+ FY ++ SFP + +L+TR ++  
Sbjct: 614 PPP----SKKMKLFGFKE--DPFVFIPEDDPLFPPIEKFYALDPSFPRM-NLLTRTTE-G 673

Query: 690 HVKRIYYISKSGKDVLELNFSVGQQLKITSIGLKMFERQSSREGTSAPCLFRISSEGLPV 749
             +++Y +SK  ++VL  N    +++K+ + G+K++ R +S  G    C FR++ EG+  
Sbjct: 674 KKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWCRNNS--GEEFDCAFRLAQEGIYT 689

Query: 750 ILPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNLMLGCCVIVLGTGSNTSS 809
           + P+I  +I+T+S  D K LL  ++  +      A+ +   +L  G  V+     S  + 
Sbjct: 734 LYPFINSRIITVSMEDVKTLLTQENPFFRKLSSEAYSQ-VKDLAKGSVVLKYEPDS-ANP 689

Query: 810 DVIKVDKSTIAIGCWKGRASLSVMV 828
           D +   +  I +  W+G+AS+   V
Sbjct: 794 DTL---QCPIVLCGWRGKASIRTFV 689

BLAST of HG10016552 vs. ExPASy Swiss-Prot
Match: Q5ZLV4 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Gallus gallus OX=9031 GN=NSUN2 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.6e-112
Identity = 278/824 (33.74%), Postives = 411/824 (49.88%), Query Frame = 0

Query: 7   GGRARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWEPFESQSPAFNEYYKE 66
           G RAR +R+  +       +R +          D  G   G P      ++  F  YY+E
Sbjct: 2   GRRARDRRRQLQPQ-----QRRERSGGGGGGGDDQAGWAGGYP--EIVKENELFERYYRE 61

Query: 67  QGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTEGDEADA 126
           Q IV   EWDAF+  LR+PLPA  RI        EI   L+  + + LQ    +G + + 
Sbjct: 62  QRIVPDGEWDAFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEM 121

Query: 127 IRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDV 186
            + L WYP+ LAWH+N SR  LRK+  LERFH+FL  E E GNI+RQEAVSM+PPL L+V
Sbjct: 122 PQALSWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNV 181

Query: 187 HPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMCT 246
           +P+H +LDMCAAPGSKT QL+E++H        P G V+AND+D +RC LL+HQ KR+ +
Sbjct: 182 NPDHKILDMCAAPGSKTAQLIEMLHADMNV-PFPKGFVIANDVDNKRCYLLVHQAKRLNS 241

Query: 247 ANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDGTLRKAP 306
             ++V NH+A   P  +             +V      L +DR+LCDVPCSGDGT+RK  
Sbjct: 242 PCIMVVNHDASSIPNLQV------------DVDGRKETLFYDRILCDVPCSGDGTMRKNI 301

Query: 307 DIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRRSGG 366
           D+W+KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NP+ENEAV+A LL +S G
Sbjct: 302 DVWKKWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQG 361

Query: 367 SLELVDVSNELPQLVRRPGLKKWKVRDR-GEWSASYKEVEGRQSVAIPSMFPSGRGRKNQ 426
           +LEL DVS+ELP L R PG+ KWKV  + G+W   +K+V                     
Sbjct: 362 ALELADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDV--------------------- 421

Query: 427 ADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFF 486
             SN  +++R                 TM P+ E +++    +ERC+RI+PH QN+G FF
Sbjct: 422 -PSNRQTQIR----------------PTMFPIKEEEKLKAMNLERCIRILPHHQNTGGFF 481

Query: 487 IAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDESPSEVGIDSN 546
           +AVL K +P+P             +   KLP    D E                  + + 
Sbjct: 482 VAVLIKKSPMP-------WNKRQPKVHQKLPGKTEDTE------------------VTAT 541

Query: 547 NQGD-SNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDNEVEENNLE 606
           N GD S  A  KP           A+  + K+V   + Q S+ +   K V        ++
Sbjct: 542 NAGDGSEDATEKPTL---------AEDEEPKKV--QELQNSDTEQSKKGVCGPPPSKKMK 601

Query: 607 VAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDP-VVLFKDETVINSIKTFYG 666
           +   K+                                 DP V L +D+ +   I+ FY 
Sbjct: 602 LFGFKE---------------------------------DPFVFLPEDDPLFLPIQKFYA 661

Query: 667 INESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLKMFERQSS 726
           ++ SFP + +L+TR  +    +++Y +SK  ++VL  N    +++K+ + G+K++ R S 
Sbjct: 662 LDPSFPKM-NLLTRTQE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWSRNS- 686

Query: 727 REGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKAS 786
            +G    C FR++ EG+  + P+I  +I+ +   D K LL  ++   + F  +    K  
Sbjct: 722 -DGEQFGCAFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQENPFLSKF-SSETQRKVK 686

Query: 787 NLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMV 828
           ++ +G  V+          D+    +  I +  W+G+ SL   V
Sbjct: 782 DMAMGSIVLKYEPDPEKPDDL----QCPIVLCGWQGKTSLRAFV 686

BLAST of HG10016552 vs. ExPASy Swiss-Prot
Match: Q08J23 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Homo sapiens OX=9606 GN=NSUN2 PE=1 SV=2)

HSP 1 Score: 404.8 bits (1039), Expect = 2.3e-111
Identity = 282/837 (33.69%), Postives = 415/837 (49.58%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWE----PFESQ 60
           MG + RG R + Q++                P  A D  +  G      WE        +
Sbjct: 1   MGRRSRGRRLQQQQR----------------PEDAEDGAEGGGKRGEAGWEGGYPEIVKE 60

Query: 61  SPAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQA 120
           +  F  YY+E  IV   EW  F++ LR+PLPA  RI       +EI   L+N + K L+ 
Sbjct: 61  NKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELED 120

Query: 121 EVTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAV 180
              +G + +  +PL WYP+ LAWH+N SR  LRK+  LE+FH+FL  E E GNI+RQEAV
Sbjct: 121 LEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAV 180

Query: 181 SMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNL 240
           SM+PPL L+V P+H +LDMCAAPGSKT QL+E++H        P+G V+AND+D +RC L
Sbjct: 181 SMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNV-PFPEGFVIANDVDNKRCYL 240

Query: 241 LIHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPC 300
           L+HQ KR+ +  ++V NH+A   P  +             +V      L +DR+LCDVPC
Sbjct: 241 LVHQAKRLSSPCIMVVNHDASSIPRLQI------------DVDGRKEILFYDRILCDVPC 300

Query: 301 SGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAV 360
           SGDGT+RK  D+W+KW +     LHGLQ++I  RGA  L  GGRMVYSTCS+NP+E+EAV
Sbjct: 301 SGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAV 360

Query: 361 VAELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDR-GEWSASYKEV-EGRQSVAIPS 420
           +A LL +S G+LEL DVSNELP L   PG+ +WKV  + G+W   +  V   R +   P+
Sbjct: 361 IASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPT 420

Query: 421 MFPSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRI 480
           MFP     K QA                                         +ERC+RI
Sbjct: 421 MFPPKDPEKLQA---------------------------------------MHLERCLRI 480

Query: 481 VPHDQNSGAFFIAVLRKVAPLP--AAIEKQNSKSANDRSRCKL-PQTIADGESVDVDAAV 540
           +PH QN+G FF+AVL K + +P      K   KSA  R   +L P  + +G+  D     
Sbjct: 481 LPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKL- 540

Query: 541 GMDESPSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEA 600
              ESPS  G      GD+                                         
Sbjct: 541 ---ESPSFTG-----TGDT----------------------------------------- 600

Query: 601 KLVDNEVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLF-K 660
                       E+A   ++ E +    D    P  S   +K+++ G     DP V   +
Sbjct: 601 ------------EIAHATEDLENNGSKKDGVCGPPPS---KKMKLFGFKE--DPFVFIPE 660

Query: 661 DETVINSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKI 720
           D+ +   I+ FY ++ SFP + +L+TR ++    +++Y +SK  ++VL  N    +++K+
Sbjct: 661 DDPLFPPIEKFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKV 690

Query: 721 TSIGLKMFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKY 780
            + G+K++ R +S  G    C FR++ EG+  + P+I  +I+T+S  D K LL  ++  +
Sbjct: 721 INTGIKVWCRNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFF 690

Query: 781 ADFVDAAFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMV 828
                  + + A +L  G  V+     S  + D +   +  I +  W+G+AS+   V
Sbjct: 781 RKLSSETYSQ-AKDLAKGSIVLKYEPDS-ANPDAL---QCPIVLCGWRGKASIRTFV 690

BLAST of HG10016552 vs. ExPASy Swiss-Prot
Match: Q28E61 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus tropicalis OX=8364 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 3.2e-108
Identity = 258/779 (33.12%), Postives = 399/779 (51.22%), Query Frame = 0

Query: 56  QSPAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQ 115
           ++  F  YY+E  IV   EWD F+  LR+PLPA  RI       +EI   L+  + K LQ
Sbjct: 46  ENKLFEHYYQELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQ 105

Query: 116 AEVTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEA 175
               +G + +A +PL WYP+ LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEA
Sbjct: 106 DIEVDGQKIEAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEA 165

Query: 176 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCN 235
           VSM+PP+ L V P+H +LDMCAAPGSKT Q++E++H        P+G V+AND+D +RC 
Sbjct: 166 VSMIPPVLLKVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCY 225

Query: 236 LLIHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVP 295
           LL+HQ KR+ +  ++V NH+A   P      N         EV      L +DR+LCDVP
Sbjct: 226 LLVHQAKRLNSPCIMVVNHDASSIPRLLVENN------GSREV------LYYDRILCDVP 285

Query: 296 CSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEA 355
           CSGDGTLRK  D+W+KW +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EA
Sbjct: 286 CSGDGTLRKNIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEA 345

Query: 356 VVAELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDR-GEWSASYKEVEGRQSVAIPS 415
           V+A LL +S GSLEL DV++E+P L   PG+ +WKV  + G W   ++++          
Sbjct: 346 VIASLLDKSEGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDI---------- 405

Query: 416 MFPSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRI 475
             P+ R          ++++R                 TM P  + +++    + RCMRI
Sbjct: 406 --PTSR----------HTQIR----------------PTMFPPKDEEKLKSMNLNRCMRI 465

Query: 476 VPHDQNSGAFFIAVLRKVAPLP-----AAIEKQNSKSANDRSRCKLPQTIADGESVDVDA 535
           +PH QN+G FF+AVL K AP+P       ++++   SA D S    P+ +        + 
Sbjct: 466 LPHHQNTGGFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELV----KAVTEN 525

Query: 536 AVGMDESPSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADL 595
           + GM + P+   +D+ N                                           
Sbjct: 526 SAGMADEPA---VDTENG------------------------------------------ 585

Query: 596 EAKLVDNEVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDP-VVL 655
           E K   N+ + +  ++      S++ K    +E                     DP V +
Sbjct: 586 ETKPCTNQSDSSKTDIVCCPPPSKKMKLFGFKE---------------------DPFVFV 645

Query: 656 FKDETVINSIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQL 715
            +D+ + + I+TFY ++ SFP   +L+TR  +    +++Y +SK  ++VL  N    +++
Sbjct: 646 SEDDPIFDPIQTFYALDPSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKM 691

Query: 716 KITSIGLKMFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSI 775
           K+ + G+K+  R  + +G    C +R++ EG+  + P+I  +I+T+S  D K LL  ++ 
Sbjct: 706 KVINTGIKVLCR--NNDGEQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENP 691

Query: 776 KYADFVDAAFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMV 828
             + F      ++A+N  +G  V+           +    +  I +  W+G+ S+   V
Sbjct: 766 FLSKF-SKETQKQANNFDMGSIVLKYEPDPQEPETL----QCPIVLCGWRGKTSIRSFV 691

BLAST of HG10016552 vs. ExPASy Swiss-Prot
Match: Q4V7N2 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus laevis OX=8355 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.6e-107
Identity = 266/822 (32.36%), Postives = 414/822 (50.36%), Query Frame = 0

Query: 18  RDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWE----PFESQSPAFNEYYKEQGIVTSE 77
           R NR N  +R +    +  + + A+       WE        ++  F  YY+E  IV   
Sbjct: 3   RKNRRNRQRRTEQRSPAEEERRKAREQA---AWEGGYPEIIKENKLFEHYYQELKIVPDG 62

Query: 78  EWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTEGDEADAIRPLPWY 137
           EWD F++ LR+PLPA  RI       +EI   L+  + K L     +G + +A +PL WY
Sbjct: 63  EWDKFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDIEVDGQKIEAPQPLSWY 122

Query: 138 PDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNHYVL 197
           P+ LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+PP+ L+V P+H +L
Sbjct: 123 PEELAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMIPPVLLNVQPHHKIL 182

Query: 198 DMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTKRMCTANLIVTN 257
           DMCAAPGSKT Q++E++H        P+G V+AND+D +RC LL+HQ KR+ +  ++V N
Sbjct: 183 DMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVN 242

Query: 258 HEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDGTLRKAPDIWRKWN 317
           H+A   P      N         EV      L +DR+LCDVPCSGDGT+RK  D+W+KW 
Sbjct: 243 HDASSIPRLLIENN------GSREV------LYYDRILCDVPCSGDGTMRKNIDVWKKWT 302

Query: 318 SGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRRSGGSLELVDV 377
           +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+  LL +S GSLEL DV
Sbjct: 303 TLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVSLLDKSEGSLELADV 362

Query: 378 SNELPQLVRRPGLKKWKVRDR-GEWSASYKEVEGRQSVAIPSMFPSGRGRKNQADSNHNS 437
           ++ELP L   PG+ +W+V  + G+W   +++V            P+ R          ++
Sbjct: 363 ASELPGLKWMPGITQWRVMTKEGQWFEKWEDV------------PTSR----------HT 422

Query: 438 ELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFFIAVLRKV 497
           ++R                 TM P+ + +++    + RCMRI+PH QN+G FF+AVL K 
Sbjct: 423 QIR----------------PTMFPLKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKK 482

Query: 498 APLP-----AAIEKQNSKSANDRSRC-KLPQTIADGESVDVDAAVGMDESPSEVGIDSNN 557
           AP+P       ++++   S  D S   ++ + +AD  ++  + AV  +   ++      N
Sbjct: 483 APMPWNKRQPKLQRRPPVSVCDASVAPEIVKAVADISAIADEPAVDAENGETK---PCTN 542

Query: 558 QGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDNEVEENNLEVA 617
           Q  S+  D+   C PP + +K                                     + 
Sbjct: 543 QSGSSKTDS-VCCPPPSKKMK-------------------------------------LF 602

Query: 618 AIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDP-VVLFKDETVINSIKTFYGIN 677
             K+                                 DP V L +D+ +   I+ FY ++
Sbjct: 603 GFKE---------------------------------DPFVFLSEDDPIFEPIQKFYALD 662

Query: 678 ESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLKMFERQSSRE 737
            SFP   +L+TR  +    +++Y +SK  ++VL  N    +++K+ + G+K+  R  + +
Sbjct: 663 PSFPK-KNLLTRTQE-GKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKVLCR--NND 684

Query: 738 GTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAFGEKASNL 797
           G    C +R++ EG+  + P+I  +ILT+S  D K LL  ++   + F      ++A+NL
Sbjct: 723 GEQYGCAYRLAQEGIYSLYPFINARILTVSVEDIKVLLTQENPFLSKF-SKETQKQANNL 684

Query: 798 MLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMV 828
            +G  V+           +    +  I +  W+G+ S+   V
Sbjct: 783 DMGSIVLKYEPDPQQPETL----QCPIVLCGWRGKTSIRSFV 684

BLAST of HG10016552 vs. ExPASy TrEMBL
Match: A0A0A0KGQ7 (SAM_MT_RSMB_NOP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G499770 PE=3 SV=1)

HSP 1 Score: 1511.5 bits (3912), Expect = 0.0e+00
Identity = 766/849 (90.22%), Postives = 794/849 (93.52%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDN-KDAKGNGNGNPWEPFESQSPA 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSANDN KD  GNGNG PWEPFESQSPA
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANDNDKDGNGNGNGKPWEPFESQSPA 60

Query: 61  FNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVT 120
           FNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAEVT
Sbjct: 61  FNEYYKEQFIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAEVT 120

Query: 121 EGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 180
           EGDEA AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV
Sbjct: 121 EGDEAVAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMV 180

Query: 181 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIH 240
           PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLLIH
Sbjct: 181 PPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLLIH 240

Query: 241 QTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGD 300
           QTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASA+GSEVKP NTQLTFDRVLCDVPCSGD
Sbjct: 241 QTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASASGSEVKPHNTQLTFDRVLCDVPCSGD 300

Query: 301 GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 360
           GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE
Sbjct: 301 GTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAE 360

Query: 361 LLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMFPS 420
           LLRRSGGSLEL+DVSNELPQLVRRPGLKKW+VRDRG W ASYKE +EGRQSVAIPSMFPS
Sbjct: 361 LLRRSGGSLELIDVSNELPQLVRRPGLKKWRVRDRGVWLASYKEIIEGRQSVAIPSMFPS 420

Query: 421 GRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHD 480
           GRGRKNQAD+N NSEL  NHLDGSIGSS DVSE  MDPV  FDEVCEFPIERCMRIVPHD
Sbjct: 421 GRGRKNQADNNDNSELGVNHLDGSIGSSVDVSEPIMDPVDGFDEVCEFPIERCMRIVPHD 480

Query: 481 QNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDESPS 540
           QNSGAFFIAVLRK+APLPA  EKQ SKS+NDRSRCKLPQTIADGESV+VDAAVGMDES S
Sbjct: 481 QNSGAFFIAVLRKIAPLPATFEKQTSKSSNDRSRCKLPQTIADGESVEVDAAVGMDESSS 540

Query: 541 EVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDNEV 600
           E GI SNNQG+ +SAD KPACE PV D KDADGLDT          +  D EAKLVDNEV
Sbjct: 541 EAGIISNNQGEPSSAD-KPACELPVHDNKDADGLDT----------NAGDSEAKLVDNEV 600

Query: 601 EENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVINSI 660
           +ENNLEVAAIK  SEESKQPDD E+DP KSS KRKLQIQGKW+GVDPVVLF DETVI+S+
Sbjct: 601 DENNLEVAAIKIYSEESKQPDDGEVDPTKSSAKRKLQIQGKWKGVDPVVLFNDETVIDSV 660

Query: 661 KTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLKMF 720
           KTFYGI+ESFPL+GHLVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLKMF
Sbjct: 661 KTFYGIDESFPLVGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKMF 720

Query: 721 ERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDAAF 780
           ERQSSREG+SAPCLFRISSEGLPVILPYITKQILT+SPVDFKHLLQYKS+KYADFVD+AF
Sbjct: 721 ERQSSREGSSAPCLFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSVKYADFVDSAF 780

Query: 781 GEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELLER 840
           GEKASNLMLGCCV+VLGTG+NTSSD IKVD STIAIGCWKGRASLSVMVTAIDCQELLER
Sbjct: 781 GEKASNLMLGCCVVVLGTGTNTSSDDIKVDSSTIAIGCWKGRASLSVMVTAIDCQELLER 838

Query: 841 LSNRLENDL 848
           LSNRLENDL
Sbjct: 841 LSNRLENDL 838

BLAST of HG10016552 vs. ExPASy TrEMBL
Match: A0A5A7T0G4 (tRNA (Cytosine(34)-C(5))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G001170 PE=3 SV=1)

HSP 1 Score: 1495.3 bits (3870), Expect = 0.0e+00
Identity = 759/851 (89.19%), Postives = 793/851 (93.18%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNK---DAKGNGNGNPWEPFESQS 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSAN N    +  GNGNGNPWEPFESQS
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANHNDKDGNGNGNGNGNPWEPFESQS 60

Query: 61  PAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAE 120
           PAFNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAE
Sbjct: 61  PAFNEYYKEQSIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAE 120

Query: 121 VTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180
           VTE DEA+AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS
Sbjct: 121 VTESDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180

Query: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLL 240
           MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLL
Sbjct: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLL 240

Query: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCS 300
           IHQTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASAAGSEVKP NTQLTFDRVLCDVPCS
Sbjct: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASAAGSEVKPHNTQLTFDRVLCDVPCS 300

Query: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360
           GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV
Sbjct: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360

Query: 361 AELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMF 420
           AELLR+SGGSLEL+DVSNELPQLVRRPGLKKW+V DRG W ASYKE +EGRQSVAIPSMF
Sbjct: 361 AELLRKSGGSLELIDVSNELPQLVRRPGLKKWRVLDRGVWLASYKEIIEGRQSVAIPSMF 420

Query: 421 PSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVP 480
           PSGRGRKNQAD+N NSEL GNHLDGSIGSS DVSE T+DPV  FDEVCEFPIERCMRIVP
Sbjct: 421 PSGRGRKNQADNNDNSELGGNHLDGSIGSSVDVSEPTVDPVDGFDEVCEFPIERCMRIVP 480

Query: 481 HDQNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           HDQNSGAFFIAVLRK+APLPA  EKQ SKSANDRSRCKLPQTIADGE+VDVDAAVGMDES
Sbjct: 481 HDQNSGAFFIAVLRKIAPLPATFEKQTSKSANDRSRCKLPQTIADGENVDVDAAVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           P+E GI SNNQG+S+SAD KPACE P+QD KDA+GL+T          +  D EAKLVDN
Sbjct: 541 PTEAGIISNNQGESSSAD-KPACELPIQDNKDAEGLNT----------NAGDSEAKLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV+E     AAIK  SEESKQPDD E+DPKKS+ KRKLQIQGKW+GVDPVVLFKDETVI+
Sbjct: 601 EVDEK----AAIKIYSEESKQPDDGEVDPKKSAAKRKLQIQGKWKGVDPVVLFKDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGI+ESFPL+G LVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLK
Sbjct: 661 SIKTFYGIDESFPLVGQLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+SAPC FRISSEGLPVILPYITKQILT+SPVDFKHLLQYKSIKY+DFVD+
Sbjct: 721 MFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYSDFVDS 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTG+NTSSDVIKVD STIAIGCWKGRASLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGTNTSSDVIKVDSSTIAIGCWKGRASLSVMVTAIDCQELL 836

Query: 841 ERLSNRLENDL 848
           ERLSNRLEN+L
Sbjct: 841 ERLSNRLENNL 836

BLAST of HG10016552 vs. ExPASy TrEMBL
Match: A0A1S3B222 (tRNA (Cytosine(34)-C(5))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103484954 PE=3 SV=1)

HSP 1 Score: 1495.3 bits (3870), Expect = 0.0e+00
Identity = 759/851 (89.19%), Postives = 793/851 (93.18%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNK---DAKGNGNGNPWEPFESQS 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSAN N    +  GNGNGNPWEPFESQS
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANHNDKDGNGNGNGNGNPWEPFESQS 60

Query: 61  PAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAE 120
           PAFNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAE
Sbjct: 61  PAFNEYYKEQSIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAE 120

Query: 121 VTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180
           VTE DEA+AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS
Sbjct: 121 VTESDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180

Query: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLL 240
           MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLL
Sbjct: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLL 240

Query: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCS 300
           IHQTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASAAGSEVKP NTQLTFDRVLCDVPCS
Sbjct: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASAAGSEVKPHNTQLTFDRVLCDVPCS 300

Query: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360
           GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV
Sbjct: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360

Query: 361 AELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMF 420
           AELLR+SGGSLEL+DVSNELPQLVRRPGLKKW+V DRG W ASYKE +EGRQSVAIPSMF
Sbjct: 361 AELLRKSGGSLELIDVSNELPQLVRRPGLKKWRVLDRGVWLASYKEIIEGRQSVAIPSMF 420

Query: 421 PSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVP 480
           PSGRGRKNQAD+N NSEL GNHLDGSIGSS DVSE T+DPV  FDEVCEFPIERCMRIVP
Sbjct: 421 PSGRGRKNQADNNDNSELGGNHLDGSIGSSVDVSEPTVDPVDGFDEVCEFPIERCMRIVP 480

Query: 481 HDQNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           HDQNSGAFFIAVLRK+APLPA  EKQ SKSANDRSRCKLPQTIADGE+VDVDAAVGMDES
Sbjct: 481 HDQNSGAFFIAVLRKIAPLPATFEKQTSKSANDRSRCKLPQTIADGENVDVDAAVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           P+E GI SNNQG+S+SAD KPACE P+QD KDA+GL+T          +  D EAKLVDN
Sbjct: 541 PTEAGIISNNQGESSSAD-KPACELPIQDNKDAEGLNT----------NAGDSEAKLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV+E     AAIK  SEESKQPDD E+DPKKS+ KRKLQIQGKW+GVDPVVLFKDETVI+
Sbjct: 601 EVDEK----AAIKIYSEESKQPDDGEVDPKKSAAKRKLQIQGKWKGVDPVVLFKDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGI+ESFPL+G LVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLK
Sbjct: 661 SIKTFYGIDESFPLVGQLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+SAPC FRISSEGLPVILPYITKQILT+SPVDFKHLLQYKSIKY+DFVD+
Sbjct: 721 MFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYSDFVDS 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTG+NTSSDVIKVD STIAIGCWKGRASLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGTNTSSDVIKVDSSTIAIGCWKGRASLSVMVTAIDCQELL 836

Query: 841 ERLSNRLENDL 848
           ERLSNRLEN+L
Sbjct: 841 ERLSNRLENNL 836

BLAST of HG10016552 vs. ExPASy TrEMBL
Match: A0A5D3CMZ3 (tRNA (Cytosine(34)-C(5))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003000 PE=3 SV=1)

HSP 1 Score: 1493.4 bits (3865), Expect = 0.0e+00
Identity = 758/851 (89.07%), Postives = 792/851 (93.07%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNK---DAKGNGNGNPWEPFESQS 60
           MGGKGRGGRARTQRKHFRDNRENVWKRP+PDPNSAN N    +  GNGNGNPWEPFESQS
Sbjct: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPRPDPNSANHNDKDGNGNGNGNGNPWEPFESQS 60

Query: 61  PAFNEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAE 120
           PAFNEYYKEQ IVT+EEWD FIEVLRKPLPAAFRINSSGQFCEEIRAQL+NDF KSLQAE
Sbjct: 61  PAFNEYYKEQSIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCEEIRAQLQNDFMKSLQAE 120

Query: 121 VTEGDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180
           VTE DEA+AIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS
Sbjct: 121 VTESDEAEAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVS 180

Query: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLL 240
           MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPG LPDGLVVANDLDVQRCNLL
Sbjct: 181 MVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGSLPDGLVVANDLDVQRCNLL 240

Query: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCS 300
           IHQTKRMCTANLIVTNHEAQHFPGCRAHTN F+ASAAGSEVKP NTQLTFDRVLCDVPCS
Sbjct: 241 IHQTKRMCTANLIVTNHEAQHFPGCRAHTNLFNASAAGSEVKPHNTQLTFDRVLCDVPCS 300

Query: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360
           GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV
Sbjct: 301 GDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVV 360

Query: 361 AELLRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKE-VEGRQSVAIPSMF 420
           AELLR+SGGSLEL+DVSNELPQLVRRPGL KW+V DRG W ASYKE +EGRQSVAIPSMF
Sbjct: 361 AELLRKSGGSLELIDVSNELPQLVRRPGLNKWRVLDRGVWLASYKEIIEGRQSVAIPSMF 420

Query: 421 PSGRGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVP 480
           PSGRGRKNQAD+N NSEL GNHLDGSIGSS DVSE T+DPV  FDEVCEFPIERCMRIVP
Sbjct: 421 PSGRGRKNQADNNDNSELGGNHLDGSIGSSVDVSEPTVDPVDGFDEVCEFPIERCMRIVP 480

Query: 481 HDQNSGAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           HDQNSGAFFIAVLRK+APLPA  EKQ SKSANDRSRCKLPQTIADGE+VDVDAAVGMDES
Sbjct: 481 HDQNSGAFFIAVLRKIAPLPATFEKQTSKSANDRSRCKLPQTIADGENVDVDAAVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           P+E GI SNNQG+S+SAD KPACE P+QD KDA+GL+T          +  D EAKLVDN
Sbjct: 541 PTEAGIISNNQGESSSAD-KPACELPIQDNKDAEGLNT----------NAGDSEAKLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV+E     AAIK  SEESKQPDD E+DPKKS+ KRKLQIQGKW+GVDPVVLFKDETVI+
Sbjct: 601 EVDEK----AAIKIYSEESKQPDDGEVDPKKSAAKRKLQIQGKWKGVDPVVLFKDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGI+ESFPL+G LVTRNSDTNHVKRIYYISKS KDVLELNFSVGQQLKITSIGLK
Sbjct: 661 SIKTFYGIDESFPLVGQLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+SAPC FRISSEGLPVILPYITKQILT+SPVDFKHLLQYKSIKY+DFVD+
Sbjct: 721 MFERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLQYKSIKYSDFVDS 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTG+NTSSDVIKVD STIAIGCWKGRASLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGTNTSSDVIKVDSSTIAIGCWKGRASLSVMVTAIDCQELL 836

Query: 841 ERLSNRLENDL 848
           ERLSNRLEN+L
Sbjct: 841 ERLSNRLENNL 836

BLAST of HG10016552 vs. ExPASy TrEMBL
Match: A0A6J1GFP9 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1485.3 bits (3844), Expect = 0.0e+00
Identity = 747/851 (87.78%), Postives = 788/851 (92.60%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWEPFESQSPAF 60
           MGGKGRGGRARTQR HFR++RENVWKR KPD NSAND KD    GN N W PF+SQS AF
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKD----GNSNHWGPFQSQSHAF 60

Query: 61  NEYYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTE 120
           NEYYKEQGIVT+ EWD FIEVLRK LPAAFRINSSGQFC EIRAQL+NDF KSLQ+EVT+
Sbjct: 61  NEYYKEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTD 120

Query: 121 GDEADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180
           GDEA AIRPL WYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP
Sbjct: 121 GDEAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVP 180

Query: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQ 240
           PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLP GLVVAND DVQRCNLLIHQ
Sbjct: 181 PLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQ 240

Query: 241 TKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDG 300
           TKRMCT+NLIVTNHEAQHFPGCRAHTN F+ASAAGSE+ P NTQLTFDRVLCDVPCSGDG
Sbjct: 241 TKRMCTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDG 300

Query: 301 TLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360
           TLRKAPDIWR+WNSG+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL
Sbjct: 301 TLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAEL 360

Query: 361 LRRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKEV-EGRQSVAIPSMFPSG 420
           LR+S GSLELVDVSNELPQLVRRPGLKKWKVRDRG W ASYK+V + RQSVAIPSMFPSG
Sbjct: 361 LRKSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSG 420

Query: 421 RGRKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQ 480
           RGRKNQAD +HNSELRGNHLDGSIGSS DVSE TMDPVHEFDEVCEFPIERCMRIVPHDQ
Sbjct: 421 RGRKNQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQ 480

Query: 481 NSGAFFIAVLRKVAPLPAAIEK---QNSKSANDRSRCKLPQTIADGESVDVDAAVGMDES 540
           NSGAFFIAVLRKVAPLPAA EK   QN K  ND+SRCKLPQTI +G +VDV A+VGMDES
Sbjct: 481 NSGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDES 540

Query: 541 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 600
           PSEVG+DSNNQGD NSADNKPACE PVQDI+  +GLD+   DG+DKQFSEADLE  LVDN
Sbjct: 541 PSEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDN 600

Query: 601 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 660
           EV  NNLE+ AI+DN EESKQPDD EMDPKKS+VKRKLQIQGKW+GVDPVVLF+DETVI+
Sbjct: 601 EVSVNNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVID 660

Query: 661 SIKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 720
           SIKTFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKS KDVLELNF VGQQLKITSIGLK
Sbjct: 661 SIKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLK 720

Query: 721 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 780
           MFERQSSREG+ APC FRI+SEGLPVILPYITKQILTMSPVDFKHL+Q+KSIKYADF DA
Sbjct: 721 MFERQSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADA 780

Query: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 840
           AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVD+STIAIGCWKGR+SLSVMVTAIDCQELL
Sbjct: 781 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELL 840

Query: 841 ERLSNRLENDL 848
           ERLSNRL ND+
Sbjct: 841 ERLSNRLGNDV 847

BLAST of HG10016552 vs. TAIR 10
Match: AT2G22400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 924.1 bits (2387), Expect = 8.2e-269
Identity = 498/847 (58.80%), Postives = 608/847 (71.78%), Query Frame = 0

Query: 5   GRGG--RARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWEPFESQSPAFNE 64
           GRGG  R RTQRK F+++RENVWKRPK D  ++ D  D         WEP  + +P F E
Sbjct: 2   GRGGRHRGRTQRKDFKESRENVWKRPKSD--ASVDGSDNAVPEQKPTWEPIVTVNPNFEE 61

Query: 65  YYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTEGD 124
           YYK+QGIV +EEWD F+E+LRKPLPAAFR+NS+GQFC+EI + LENDF KSLQAE  EG 
Sbjct: 62  YYKKQGIVKAEEWDLFMEILRKPLPAAFRVNSNGQFCDEIISILENDFMKSLQAEAIEGG 121

Query: 125 EADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 184
           E +AI+PLPWYP NLAWHSNFSR ++RKNQ LERFHEFLKLENE+GNITRQEAVSMVPPL
Sbjct: 122 ELEAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLENEVGNITRQEAVSMVPPL 181

Query: 185 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 244
           FLDVHP+H+VLDMCAAPGSKTFQLLEIIH++S+PG LP+GLVVAND+D +R NLLIHQTK
Sbjct: 182 FLDVHPDHFVLDMCAAPGSKTFQLLEIIHEASEPGSLPNGLVVANDVDFKRSNLLIHQTK 241

Query: 245 RMCTANLIVTNHEAQHFPGCRAHTNFFSASAAG-SEVKPLNTQLTFDRVLCDVPCSGDGT 304
           RMCT+NLIVTNHE Q FPGCR   N   AS  G SE  P+N QL FDRVLCDVPCSGDGT
Sbjct: 242 RMCTSNLIVTNHEGQQFPGCR--LNKSRASEKGISENMPIN-QLAFDRVLCDVPCSGDGT 301

Query: 305 LRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELL 364
           LRKAPDIWRKWNSGMGNGLH LQ+ + MRG SLLKVGG+M+YSTCSMNPVE+EAVVAE+L
Sbjct: 302 LRKAPDIWRKWNSGMGNGLHSLQIILAMRGLSLLKVGGKMIYSTCSMNPVEDEAVVAEIL 361

Query: 365 RRSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKEV-EGRQSVAIPSMFPSGR 424
           RR G S+EL+DVS++LP+L+RRPGLK WKVRD+G W  SYK+V + R+   + SMFPSG+
Sbjct: 362 RRCGDSVELLDVSDKLPELIRRPGLKAWKVRDKGGWFTSYKDVPQNRRGGVLVSMFPSGK 421

Query: 425 GRKNQADS---NHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPH 484
             K+  ++   N N ++ G   DG      D+S     P  + +EV + P+ERCMRI+PH
Sbjct: 422 YLKDSTETTEKNENGDVNGCE-DGL--KETDISVVDATPEEQAEEVSDLPLERCMRIIPH 481

Query: 485 DQNSGAFFIAVLRKVAPLPAAIEKQNSK-SANDRSRCKLPQTIADGESVDVDAAVGMDES 544
           DQN+GAFFIAVL+K +PLP   EK N+K ++  +S     ++ +    V VDA V     
Sbjct: 482 DQNTGAFFIAVLQKKSPLPEFQEKPNTKRNSTAKSTDSTEKSPSKESVVTVDAGV----- 541

Query: 545 PSEVGIDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDN 604
           P E  ++   + DSN              I+  D L+                E K+ + 
Sbjct: 542 PDESAVEKVIEADSN--------------IEKNDSLEP---------------EKKITEG 601

Query: 605 EVEENNLEVAAIKDNSEESKQPDDREMDPKKSSVKRKLQIQGKWRGVDPVVLFKDETVIN 664
           E                     +D+E +   +  KRK+ +QGKW+G DPVV  KDETVIN
Sbjct: 602 E------------------SITEDKEANSSNAGGKRKVPMQGKWKGFDPVVFVKDETVIN 661

Query: 665 SIKTFYGI-NESFPLIGHLVTRNSDTNHVKRIYYISKSGKDVLELNFSVGQQLKITSIGL 724
            IK FYGI +ESFPL GHLV RN+DT+ VKRIYY+SKS K+VL+LNF+VGQQLKI S+GL
Sbjct: 662 GIKEFYGIKDESFPLHGHLVARNTDTSSVKRIYYVSKSVKEVLQLNFAVGQQLKIASVGL 721

Query: 725 KMFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVD 784
           KMFERQS++EG+S  C FRISSEGLPVILPYITKQ+L     DFK LLQ KSIK+ DFV+
Sbjct: 722 KMFERQSAKEGSSTLCPFRISSEGLPVILPYITKQVLYTPMADFKLLLQDKSIKFHDFVN 781

Query: 785 AAFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQEL 843
               +KA++L++G CV++L  G       +K D STIAI CW+G+ASL+VM T  DCQEL
Sbjct: 782 PQLAQKATDLVMGSCVVILSDGEVP----VKADASTIAISCWRGKASLAVMATVADCQEL 784

BLAST of HG10016552 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 822.0 bits (2122), Expect = 4.4e-238
Identity = 458/846 (54.14%), Postives = 576/846 (68.09%), Query Frame = 0

Query: 3   GKGRGGRARTQRKHFRDNRENVWKRPKPDPNSANDNKDAKGNGNGNPWEPFESQSPAFNE 62
           G+GR  R  T +++  +N          D N     K      + N  +P  S +  F E
Sbjct: 2   GRGRRHRGSTSKRNQIENSN--------DDNVRKPLKSEASFDSNNLQKPICS-NLHFEE 61

Query: 63  YYKEQGIVTSEEWDAFIEVLRKPLPAAFRINSSGQFCEEIRAQLENDFTKSLQAEVTEGD 122
           YYKEQ IV ++EWD+F+E LRKPLPAAFR+ S+GQFC+EIR +LENDF  SLQAE  E  
Sbjct: 62  YYKEQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIESG 121

Query: 123 EADAIRPLPWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 182
           E +AI+PLPWYP NLAWHSNFSR ++RKNQ LERFHEFLKLE E GN+TRQE+VSMVPPL
Sbjct: 122 ELEAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVPPL 181

Query: 183 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCNLLIHQTK 242
           FLDVHP+H+VLDMCAAPGSKTFQLLEIIH+SS+PG LP+G+VVAND+D +R NLLIHQTK
Sbjct: 182 FLDVHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQTK 241

Query: 243 RMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVPCSGDGTL 302
           R CT NL+VTN+E QHFP C        AS       P++ QL FDRVLCDVPCSGDGTL
Sbjct: 242 RTCTTNLMVTNNEGQHFPSCNTKRTLSVASETNPH--PID-QLLFDRVLCDVPCSGDGTL 301

Query: 303 RKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 362
           RKAPDIWR+WNSG GNGLH LQV + MRG SLLKVGGRMVYSTCSMNP+E+EAVVAE+LR
Sbjct: 302 RKAPDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILR 361

Query: 363 RSGGSLELVDVSNELPQLVRRPGLKKWKVRDRGEWSASYKEV-EGRQSVAIPSMFPSGRG 422
           R G S+ELVDVS++LP+L+RRPGL KWKV DRG W  SYK+V + ++   + SMFPSG+ 
Sbjct: 362 RCGCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSGKS 421

Query: 423 RKNQADSNHNSELRGNHLDGSIGSSADVSEQTMDPVHEFDEVCEFPIERCMRIVPHDQNS 482
            K+ +   ++ E             A +S          +EVC+ P+E CMRI+PHDQN+
Sbjct: 422 DKDSSGGKNSYE-----------EMASISSD-----ESAEEVCDLPLELCMRILPHDQNT 481

Query: 483 GAFFIAVLRKVAPLPAAIEKQNSKSANDRSRCKLPQTIADGESVDVDAAVGMDESPSEVG 542
           G FFIAVL KV+PLP   EK   K                                    
Sbjct: 482 GGFFIAVLHKVSPLPDFQEKVTQKR----------------------------------- 541

Query: 543 IDSNNQGDSNSADNKPACEPPVQDIKDADGLDTKEVDGMDKQFSEADLEAKLVDNEVEEN 602
            + + + + NS++   + E        +D + TK  +G +    E  LEA + +N  E  
Sbjct: 542 -NLSTRNNINSSEKSSSYEAV------SDTVVTKPEEGTE----EIVLEATVSENGFEPP 601

Query: 603 NLEVAAIKDNSEESKQPDDREMDPKKSSVKRKL-QIQGKWRGVDPVVLFKDETVINSIKT 662
            +E    +   E +++ DD E        KR++  +QGKW+G+ PVV  +D+T IN IKT
Sbjct: 602 EMESTWNEGIVELAQKLDDIE-------GKREVPSMQGKWKGLYPVVFLRDQTGINGIKT 661

Query: 663 FYGI-NESFPLIGHLVTRNSDTN---HVKRIYYISKSGKDVLELNFSVGQQLKITSIGLK 722
           FYGI +ESFPL GHLVTRNSD +   +VKRIYY+SK+ K+VLELNF+VG+ LKI+S+GLK
Sbjct: 662 FYGIKDESFPLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSVGLK 721

Query: 723 MFERQSSREGTSAPCLFRISSEGLPVILPYITKQILTMSPVDFKHLLQYKSIKYADFVDA 782
           MFE+QS++E  +  C FRI+SEGLPVILPY+TKQIL  +  DFK+LLQ+KSIK+ DF+  
Sbjct: 722 MFEKQSAKECEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDFIHP 766

Query: 783 AFGEKASNLMLGCCVIVLGTGSNTSSDVIKVDKSTIAIGCWKGRASLSVMVTAIDCQELL 842
             GEKA+ L LG CV+VL   S   S+  KV+ STIAIGCWKG+ASL+VMVT +DC +L+
Sbjct: 782 QIGEKAAELALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCHQLI 766

BLAST of HG10016552 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.9e-15
Identity = 59/195 (30.26%), Postives = 86/195 (44.10%), Query Frame = 0

Query: 173 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQ 232
           Q A S +P + L    N  ++D+ AAPG KT  +  ++  +        GL+ AN++ V 
Sbjct: 344 QGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNT--------GLIYANEMKVP 403

Query: 233 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLC 292
           R   L     RM   N IV N++                   G E+  +  Q T DRVL 
Sbjct: 404 RLKSLTANLHRMGVTNTIVCNYD-------------------GRELPKVLGQNTVDRVLL 463

Query: 293 DVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 352
           D PCSG G + K   +              LQ Q+ +    ++    K GG +VYSTCS+
Sbjct: 464 DAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSI 511

Query: 353 NPVENEAVVAELLRR 364
              ENEAV+   L++
Sbjct: 524 MVTENEAVIDYALKK 511

BLAST of HG10016552 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 5.5e-15
Identity = 59/194 (30.41%), Postives = 86/194 (44.33%), Query Frame = 0

Query: 173 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQ 232
           Q A S +P + L       V+DM AAPG KT  +  ++  +        G++ AN++ V 
Sbjct: 327 QSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNT--------GIIYANEMKVP 386

Query: 233 RCNLLIHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLC 292
           R   L     RM   N IV N++                   G E+  +  Q + DRVL 
Sbjct: 387 RLKSLSANLHRMGVTNTIVCNYD-------------------GRELTKVLGQSSVDRVLL 446

Query: 293 DVPCSGDGTLRKAPDIWRKWNSGMGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 352
           D PCSG G + K   +    ++        LQ Q+ +    L+    K GG +VYSTCS+
Sbjct: 447 DAPCSGTGVISKDESVKTSKSADDIKKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSV 493

Query: 353 NPVENEAVVAELLR 363
              ENEAV+   L+
Sbjct: 507 MIPENEAVIDYALK 493

BLAST of HG10016552 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 75.9 bits (185), Expect = 1.8e-13
Identity = 60/190 (31.58%), Postives = 85/190 (44.74%), Query Frame = 0

Query: 176 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPDGLVVANDLDVQRCN 235
           VS+V P      P   ++D CAAPG KT  +   +           G++ A D++  R  
Sbjct: 323 VSVVKP-----QPGERIMDACAAPGGKTLFMASCLK--------GQGMIYAMDVNEGRLR 382

Query: 236 LLIHQTKRMCTANLIVTNHEAQHFPGCRAHTNFFSASAAGSEVKPLNTQLTFDRVLCDVP 295
           +L    K      LI T H         A TN                ++ +D+VL D P
Sbjct: 383 ILGETAKSHQVDGLITTIHSDLR---VFAETN----------------EVQYDKVLLDAP 442

Query: 296 CSGDGTLRKAPDIWRKWNSGMGNGLH--GLQVQIGMRGASLLKVGGRMVYSTCSMNPVEN 355
           CSG G L K  D+  +WN  + + L    LQ ++    + L+K GG +VYSTCS++P EN
Sbjct: 443 CSGLGVLSKRADL--RWNRKLEDMLELTKLQDELLDSASKLVKHGGVLVYSTCSIDPEEN 478

Query: 356 EAVVAELLRR 364
           E  V   L R
Sbjct: 503 EGRVEAFLLR 478