HG10016516 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview


Name	HG10016516
Type	gene
Organism	Lagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description	L-ascorbate oxidase
Location	Chr03: 5584640 .. 5586644 (-)
RNA-Seq Expression	HG10016516
Synteny	HG10016516

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCGTTGTTACAAAGTAGACAATACCAGTTCATTCTTCTCTGCTACTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGACCTATAAATGGGAAGTGAAGTCCGAATTGAAGTCCCCTGACTGCTTTAAAAAACTTACCATGACCATTAATGGCCGATCTCCCGGACCCACAATCTACGCCCACCAAGGCGACACCGTCATCGTTGAGCTCAAGAACAGCTTGAGCACCGAAAACGTCGCCATCCATTGGCATGGAATCCGACAGGTTCGCCAAATTTCATCTCTCTCCAATTACTTAGATGATTTTGTATAATTTTTCTTTTCCTGAAGTTTTTGTACTTTGTGTAATATAATTGAAGATTGGAACACCTTGGAGCGATGGGACAGAAGGTGTCACTCAATGTCCAATTTTACCTGGTGAAACCTTCAGATATCAGTTCGTTGTAGACAGGGTAAGAGCTGGACCCACTGGTCTTGTCTTGTGCCTCTCAGGAACTCCATTATTTTGGGTTTGACCTTTATGAGTTTTGAAATCTGCAGCCAGGGACTTATCTGTACCATGCCCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGCTTCCAGAAGGACAGTCAGAGCCTTTTTCCTACGATCATGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCCATGAACACGCCGCTGGATTGGCTTCCCCTGGCAATGGCTTCACCTGGGTGGGAGAGCCTGATTCACTTTTGATACAAGGAAGAGGGAGATTCAATTGCTCTGTCTTGGGATCTTCCAGCTTAGCCTCTGCTACTTGTAATTCATCCAGCCCAGAATGTGTTCTTCATCCATTAACGGTCGTACCAGGCAAAACTTACCGATTGAGGGTCTCTAGTCTTACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGTAATTACAAAATCATCTACTTAATTTCATTTCCTTCCTTGAAATTCTTCTGAAATGGCTTAATCTCTCTGTTTCGGAAACAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTCCAAGTGAACAACTTGTTCATATACTCAGGAGAAACATATTCCGTGTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGACAAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTATTACCCAAATCACCAGAGTAAACTCCCTCCGACAACGCCTCCGGCAGCACCTGCTTGGAACGATGTTGCACCTCGATTGGCTCAAAGCCACGCCATCAAAGCCCGGCAAGGGTTTATCCACGCGCCGCCCAAAGCCGCCGACAAAGTGATCGTGCTTCTCAACACCCAAAACACTGTGAATGGTCGCCGCCGGTGGTCGTTAAATAACGTTTCCTTTAACATGCCCCACACCCCTTACCTTATTGCTCTGAAGCACAATCTTCTCCATACCTTCAGCCAGGAAAAACCACCCACCGGATACGATTTCAAGAACTACGACATTTTCAATCCAGCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATATTCCGGCTGGACTTCAATTCAACTGTGGACATCATTCTGCAGAACGCCAACATGATGAGCCCAAACAACAGTGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTGTTGGGCTACGGGGAAGGAAAATTCGATATCTATAAAGACCCAAGCAAATACAATTTGGAAAATCCGATCATGAAGAACACAGTGCCGTTGCACCCTTATGGATGGACGGCCTTGAGATTTGTGGCTGATAACCCTGGGGTTTGGGCTTTCCACTGCCACATCGACGCCCATTTCTTTATGGGAATGGGAGTTGTCTTTGCAGAAGGGATTGACAGGGTTCAGAAATTGCCTACTTCCATTATGGGGTGCGGCGAGAGCAAGAGATTCTTAAGGCCTTAA

mRNA sequence

ATGGCTTCGTTGTTACAAAGTAGACAATACCAGTTCATTCTTCTCTGCTACTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGACCTATAAATGGGAAGTGAAGTCCGAATTGAAGTCCCCTGACTGCTTTAAAAAACTTACCATGACCATTAATGGCCGATCTCCCGGACCCACAATCTACGCCCACCAAGGCGACACCGTCATCGTTGAGCTCAAGAACAGCTTGAGCACCGAAAACGTCGCCATCCATTGGCATGGAATCCGACAGATTGGAACACCTTGGAGCGATGGGACAGAAGGTGTCACTCAATGTCCAATTTTACCTGGTGAAACCTTCAGATATCAGTTCGTTGTAGACAGGCCAGGGACTTATCTGTACCATGCCCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGCTTCCAGAAGGACAGTCAGAGCCTTTTTCCTACGATCATGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCCATGAACACGCCGCTGGATTGGCTTCCCCTGGCAATGGCTTCACCTGGGTGGGAGAGCCTGATTCACTTTTGATACAAGGAAGAGGGAGATTCAATTGCTCTGTCTTGGGATCTTCCAGCTTAGCCTCTGCTACTTGTAATTCATCCAGCCCAGAATGTGTTCTTCATCCATTAACGGTCGTACCAGGCAAAACTTACCGATTGAGGGTCTCTAGTCTTACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTCCAAGTGAACAACTTGTTCATATACTCAGGAGAAACATATTCCGTGTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGACAAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTATTACCCAAATCACCAGAGTAAACTCCCTCCGACAACGCCTCCGGCAGCACCTGCTTGGAACGATGTTGCACCTCGATTGGCTCAAAGCCACGCCATCAAAGCCCGGCAAGGGTTTATCCACGCGCCGCCCAAAGCCGCCGACAAAGTGATCGTGCTTCTCAACACCCAAAACACTGTGAATGGTCGCCGCCGGTGGTCGTTAAATAACGTTTCCTTTAACATGCCCCACACCCCTTACCTTATTGCTCTGAAGCACAATCTTCTCCATACCTTCAGCCAGGAAAAACCACCCACCGGATACGATTTCAAGAACTACGACATTTTCAATCCAGCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATATTCCGGCTGGACTTCAATTCAACTGTGGACATCATTCTGCAGAACGCCAACATGATGAGCCCAAACAACAGTGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTGTTGGGCTACGGGGAAGGAAAATTCGATATCTATAAAGACCCAAGCAAATACAATTTGGAAAATCCGATCATGAAGAACACAGTGCCGTTGCACCCTTATGGATGGACGGCCTTGAGATTTGTGGCTGATAACCCTGGGGTTTGGGCTTTCCACTGCCACATCGACGCCCATTTCTTTATGGGAATGGGAGTTGTCTTTGCAGAAGGGATTGACAGGGTTCAGAAATTGCCTACTTCCATTATGGGGTGCGGCGAGAGCAAGAGATTCTTAAGGCCTTAA

Coding sequence (CDS)

ATGGCTTCGTTGTTACAAAGTAGACAATACCAGTTCATTCTTCTCTGCTACTTCTTGTTCTTATTACAATCTCCAACTGCAGAATCAAGAACTCTGACCTATAAATGGGAAGTGAAGTCCGAATTGAAGTCCCCTGACTGCTTTAAAAAACTTACCATGACCATTAATGGCCGATCTCCCGGACCCACAATCTACGCCCACCAAGGCGACACCGTCATCGTTGAGCTCAAGAACAGCTTGAGCACCGAAAACGTCGCCATCCATTGGCATGGAATCCGACAGATTGGAACACCTTGGAGCGATGGGACAGAAGGTGTCACTCAATGTCCAATTTTACCTGGTGAAACCTTCAGATATCAGTTCGTTGTAGACAGGCCAGGGACTTATCTGTACCATGCCCATTACGGTATGCAAAGAGAAGATGGTCTCTATGGATCCATTGTGGTTTGGCTTCCAGAAGGACAGTCAGAGCCTTTTTCCTACGATCATGATCGTAATCTCATCCTCACAGATTGGTATCATAAAAGCAGCCATGAACACGCCGCTGGATTGGCTTCCCCTGGCAATGGCTTCACCTGGGTGGGAGAGCCTGATTCACTTTTGATACAAGGAAGAGGGAGATTCAATTGCTCTGTCTTGGGATCTTCCAGCTTAGCCTCTGCTACTTGTAATTCATCCAGCCCAGAATGTGTTCTTCATCCATTAACGGTCGTACCAGGCAAAACTTACCGATTGAGGGTCTCTAGTCTTACTTCTCTTTCAGCTCTCAGTTTCCAAATTGAGGGTCACACCATGACTGTGGTTGAAGCTGATGGGCATTACGTTGAGCCATTCCAAGTGAACAACTTGTTCATATACTCAGGAGAAACATATTCCGTGTTGATAAAAGCCGATCAAGACCCTTCTAGAAACTACTGGATCACAACCAATGTGGTGAGCCGACAAAGAACCACCCCCCCTGGTTTAGCCATTTTCAATTATTACCCAAATCACCAGAGTAAACTCCCTCCGACAACGCCTCCGGCAGCACCTGCTTGGAACGATGTTGCACCTCGATTGGCTCAAAGCCACGCCATCAAAGCCCGGCAAGGGTTTATCCACGCGCCGCCCAAAGCCGCCGACAAAGTGATCGTGCTTCTCAACACCCAAAACACTGTGAATGGTCGCCGCCGGTGGTCGTTAAATAACGTTTCCTTTAACATGCCCCACACCCCTTACCTTATTGCTCTGAAGCACAATCTTCTCCATACCTTCAGCCAGGAAAAACCACCCACCGGATACGATTTCAAGAACTACGACATTTTCAATCCAGCGCCCAACCCTGAAGCGACGGTGAGCGACGCCATATTCCGGCTGGACTTCAATTCAACTGTGGACATCATTCTGCAGAACGCCAACATGATGAGCCCAAACAACAGTGAAACGCATCCTTGGCATTTGCACGGCCACGATTTCTGGGTGTTGGGCTACGGGGAAGGAAAATTCGATATCTATAAAGACCCAAGCAAATACAATTTGGAAAATCCGATCATGAAGAACACAGTGCCGTTGCACCCTTATGGATGGACGGCCTTGAGATTTGTGGCTGATAACCCTGGGGTTTGGGCTTTCCACTGCCACATCGACGCCCATTTCTTTATGGGAATGGGAGTTGTCTTTGCAGAAGGGATTGACAGGGTTCAGAAATTGCCTACTTCCATTATGGGGTGCGGCGAGAGCAAGAGATTCTTAAGGCCTTAA

Protein sequence

MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP

Homology

[Close]

BLAST of HG10016516 vs. NCBI nr
Match: XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])

HSP 1 Score: 1172.1 bits (3031), Expect = 0.0e+00
Identity = 552/579 (95.34%), Postives = 567/579 (97.93%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MASLLQSRQY  ILLC+ LFL+QSP AESRTL YKWEVKSELKSPDCFKKLTMTINGRSP
Sbjct: 18  MASLLQSRQYLLILLCFLLFLIQSPIAESRTLNYKWEVKSELKSPDCFKKLTMTINGRSP 77

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS STENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+YQ
Sbjct: 78  GPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQ 137

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPFSYD+DRNLILTDWYHK+SHEH
Sbjct: 138 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKTSHEH 197

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           AAGLA+PG+GFTWVGEPDSLLIQGRGRFNCSVLGSS+  SATCNSS+PEC+L+P+TVVPG
Sbjct: 198 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSST--SATCNSSNPECILYPITVVPG 257

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 258 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 317

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR RTTPPGLAIFNYYPNHQ KLPPTTPPA PAWNDVAPRLAQSHAIK
Sbjct: 318 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQRKLPPTTPPAPPAWNDVAPRLAQSHAIK 377

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLH FSQ
Sbjct: 378 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHAFSQ 437

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
           +KPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL
Sbjct: 438 DKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 497

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFDIYKDPSK+NLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH
Sbjct: 498 HGHDFWVLGYGEGKFDIYKDPSKFNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 557

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP
Sbjct: 558 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 594

[Close]

BLAST of HG10016516 vs. NCBI nr
Match: XP_008440636.1 (PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase [Cucumis melo var. makuwa] >TYK12674.1 L-ascorbate oxidase [Cucumis melo var. makuwa])

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 546/579 (94.30%), Postives = 558/579 (96.37%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MASLLQ +QY  +LL  FLFLLQS TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           AAGLA+PG+GFTWVGEPDSLLIQGRGRFNCSVLGSS   S TCNSSS ECVL+PLTVVPG
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLYPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR RTTPPGLAIFNYYPNHQSK PPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
           EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNANMMSPNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFDIY DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of HG10016516 vs. NCBI nr
Match: XP_004143482.1 (L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_003073 [Cucumis sativus])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 541/579 (93.44%), Postives = 554/579 (95.68%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MAS LQS +Y  ILL +  FLLQS TAESRTL YKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           A GLA+PG+GF WVGEPDSLLIQGRGRFNCS+LGSS   S TCNSSS ECVLHPLTVVPG
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSS---SGTCNSSSTECVLHPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR RTTPPGLAIFNYYPNHQS+LPPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
           EKPPTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNANMMSPNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFD+YKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of HG10016516 vs. NCBI nr
Match: KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 522/579 (90.16%), Postives = 550/579 (94.99%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           M SLLQSRQY  ILLC+F+FL +S TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQSRQYLLILLCFFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPF+YDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           +AGLA+ G+ FTWVGEPDSLLIQGRGRFNCS+LG    ASATCNSSSP+CVL+P+TVVPG
Sbjct: 181 SAGLAAAGSAFTWVGEPDSLLIQGRGRFNCSLLG----ASATCNSSSPDCVLYPITVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           +TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+
Sbjct: 241 RTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQN 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR  TTP GLAIFNYYPNHQ K PPTTPPA PAWN VAPRLAQSHAIK
Sbjct: 301 PSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
            KPPTGYDFKNY+IF P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHL
Sbjct: 421 AKPPTGYDFKNYNIFVPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI++VQKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 575

[Close]

BLAST of HG10016516 vs. NCBI nr
Match: XP_022949916.1 (L-ascorbate oxidase-like [Cucurbita moschata] >XP_022949917.1 L-ascorbate oxidase-like [Cucurbita moschata] >XP_022949918.1 L-ascorbate oxidase-like [Cucurbita moschata] >XP_022949919.1 L-ascorbate oxidase-like [Cucurbita moschata] >XP_022949920.1 L-ascorbate oxidase-like [Cucurbita moschata] >XP_022949921.1 L-ascorbate oxidase-like [Cucurbita moschata])

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 521/579 (89.98%), Postives = 542/579 (93.61%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MA LLQS QY  IL C+F+FL  S  AESRTL YKWEVK E++SPDCFKKLT+TING SP
Sbjct: 1   MAPLLQSTQYLLILFCFFVFLSASLVAESRTLFYKWEVKYEIRSPDCFKKLTITINGGSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGD VIVELKNSL++ENVA+HWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDNVIVELKNSLASENVAVHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDR GTYLYHAHYGMQR+DGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRAGTYLYHAHYGMQRQDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           A GLA+P  GF WVGEPDSLLIQGRGRF CS+LGSS LASATCNSSSP+CV +PLTVVPG
Sbjct: 181 ATGLATPDPGFQWVGEPDSLLIQGRGRFKCSLLGSSGLASATCNSSSPQCVAYPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIE HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIESHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYW TTNVVSR RTTPPGLAIFNYYPNH+ KLPPTTPPA PAWNDVAPRLAQSHAIK
Sbjct: 301 PSRNYWATTNVVSRPRTTPPGLAIFNYYPNHRRKLPPTTPPAGPAWNDVAPRLAQSHAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPK ADKVIVLLNTQNTV GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKIADKVIVLLNTQNTVKGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
            KPPTGYDFKNYDIFNPAPN EAT SDAIFRL+F STVDIILQNANMMSPN+S+THPWHL
Sbjct: 421 AKPPTGYDFKNYDIFNPAPNQEATESDAIFRLEFKSTVDIILQNANMMSPNSSDTHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFDIY+DPSKYNLENPIMKNTV LHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYRDPSKYNLENPIMKNTVALHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGID+V  LPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIDQVGNLPTSIMGCGESKRFLRP 579

[Close]

BLAST of HG10016516 vs. ExPASy Swiss-Prot
Match: P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)

HSP 1 Score: 617.8 bits (1592), Expect = 1.2e-175
Identity = 290/541 (53.60%), Postives = 369/541 (68.21%), Query Frame = 0


Query: 34  YKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENVAIHWHGIR 93
           YKW+V+    SPDC + + M ING  PGPTI A+ GD V+VEL N L TE V IHWHGI 
Sbjct: 41  YKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAGDIVVVELTNKLHTEGVVIHWHGIL 100

Query: 94  QIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPE 153
           Q GTPW+DGT  ++QC I PGETF Y+FVVD+ GTY YH H GMQR  GLYGS++V  PE
Sbjct: 101 QRGTPWADGTASISQCAINPGETFTYRFVVDKAGTYFYHGHLGMQRSAGLYGSLIVDPPE 160

Query: 154 GQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVL 213
           G+SEPF YD + NL+L+DW+H+S H+   GL+S      W+GEP S+LI G+G+F+CS+ 
Sbjct: 161 GRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--KPMRWIGEPQSILINGKGQFDCSIA 220

Query: 214 GSSSLASATCNSSSPE-CVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADG 273
              +     C  S  E C    L V P KTYR+R++S T+L++L+F I  H + VVEADG
Sbjct: 221 AKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADG 280

Query: 274 HYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQ 333
           +YV+PF  +++ IYSGE+YSVLI  DQ+P  NYW++  V +R   TPPGL + NY PN  
Sbjct: 281 NYVQPFVTSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSA 340

Query: 334 SKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRW 393
           SKLP + PP  P W D       +  I A  G    PP   ++ + LLNTQN +NG  +W
Sbjct: 341 SKLPISPPPETPHWEDFDRSKNFTFRIFAAMG-SPKPPVRYNRRLFLLNTQNRINGFMKW 400

Query: 394 SLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRL 453
           ++NNVS  +P TPYL A+K  L   F+Q  PP  +   NYDI NP PNPE T  + +++ 
Sbjct: 401 AINNVSLALPPTPYLAAMKMRLNTAFNQNPPPETFPL-NYDINNPPPNPETTTGNGVYKF 460

Query: 454 DFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMK 513
           +   TVD+ILQNANM++PN SE HPWHLHGHDFWVLGYGEGKF   +D  K NL+NP ++
Sbjct: 461 NMGETVDVILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLR 520

Query: 514 NTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGE 573
           NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVFAEG+  V  +P   + CG 
Sbjct: 521 NTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGS 577

[Close]

BLAST of HG10016516 vs. ExPASy Swiss-Prot
Match: P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)

HSP 1 Score: 611.3 bits (1575), Expect = 1.1e-173
Identity = 294/581 (50.60%), Postives = 394/581 (67.81%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYF-LFLLQSPTAE-SRTLTYKWEVKSELKSPDCFKKLTMTINGR 60
           M  + ++R+  F++L +F L L    ++E S+   YKWEV+    +PDC + + M ING+
Sbjct: 1   MLQMGKAREPNFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQ 60

Query: 61  SPGPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFR 120
            PGPTI A+ GDTV+VEL N L TE V IHWHGI Q GTPW+DGT  ++QC I PGETF 
Sbjct: 61  FPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFF 120

Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSH 180
           Y F VD PGT+ YH H GMQR  GLYGS++V  P+G+ EPF YD + NL+L+DW+H+S H
Sbjct: 121 YNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIH 180

Query: 181 EHAAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLG--SSSLASATCNSSSPECVLHPLT 240
           +   GL+S      W+GEP ++L+ GRG+F+CS+     S+L       S P C  +   
Sbjct: 181 KQEVGLSS--KPIRWIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEP-CAPYIFH 240

Query: 241 VVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIK 300
           V+P KTYR+R++S T+L+AL+F I  H + VVEADG+YV+PF  +++ IYSGE+YSVLI 
Sbjct: 241 VMPKKTYRIRIASTTALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLIT 300

Query: 301 ADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQS 360
            DQ+PS NYW++     R   TPPGL + NY PN  SKLP + PP  PAW+D       +
Sbjct: 301 TDQNPSENYWVSVGTRGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFT 360

Query: 361 HAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLH 420
           + I A  G    PP  +++ I LLNTQN +NG  +W++N+VS  +P TPYL A+K NLLH
Sbjct: 361 YRITAAMG-SPKPPVKSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLLH 420

Query: 421 TFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETH 480
            F Q  PP  +  ++YDI  P  N +  + + +++      VD+ILQNANMM  N SE H
Sbjct: 421 AFDQNPPPEVFP-EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIH 480

Query: 481 PWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWA 540
           PWHLHGHDFWVLGYG+GKF   ++ S  NL+NP ++NTV + PYGWTA+RFVADNPGVWA
Sbjct: 481 PWHLHGHDFWVLGYGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWA 540

Query: 541 FHCHIDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFL 578
           FHCHI+ H  MGMGVVFAEG+++V ++PT  + CG + + L
Sbjct: 541 FHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGGTAKSL 575

[Close]

BLAST of HG10016516 vs. ExPASy Swiss-Prot
Match: P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 5.6e-173
Identity = 282/545 (51.74%), Postives = 373/545 (68.44%), Query Frame = 0


Query: 34  YKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENVAIHWHGIR 93
           YKWEV+    +P+C + + M ING+ PGPTI A+ GD+V+VEL N L TE V IHWHGI 
Sbjct: 6   YKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIHWHGIL 65

Query: 94  QIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPE 153
           Q GTPW+DGT  ++QC I PGETF Y F VD PGT+ YH H GMQR  GLYGS++V  P+
Sbjct: 66  QRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQ 125

Query: 154 GQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVL 213
           G+ EPF YD + NL+L+DW+H+S H+   GL+S      W+GEP ++L+ GRG+F+CS+ 
Sbjct: 126 GKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQTILLNGRGQFDCSIA 185

Query: 214 GSSSLASATCN-SSSPECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTVVEADG 273
                    C    S  C  +   V P KTYR+R++S T+L+AL+F I  H + VVEADG
Sbjct: 186 AKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADG 245

Query: 274 HYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQ 333
           +YV+PF  +++ IYSGE+YSVLI  DQ+PS NYW++    +R   TPPGL + NY PN  
Sbjct: 246 NYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSV 305

Query: 334 SKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQNTVNGRRRW 393
           SKLP + PP  PAW+D       ++ I A  G    PP   ++ I LLNTQN +NG  +W
Sbjct: 306 SKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVINGYVKW 365

Query: 394 SLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSDAIFRL 453
           ++N+VS  +P TPYL A+K+NLLH F Q  PP  +  ++YDI  P  N +  + + +++ 
Sbjct: 366 AINDVSLALPPTPYLGAMKYNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGNGVYQF 425

Query: 454 DFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKYNLENPIMK 513
                VD+ILQNANMM  N SETHPWHLHGHDFWVLGYG+GKF   ++ S  NL+NP ++
Sbjct: 426 KIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSLNLKNPPLR 485

Query: 514 NTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGE 573
           NTV + PYGWTA+RFVADNPGVWAFHCHI+ H  MGMGVVFAEG+++V ++PT  + CG 
Sbjct: 486 NTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKALACGG 545

Query: 574 SKRFL 578
           + + L
Sbjct: 546 TAKSL 545

[Close]

BLAST of HG10016516 vs. ExPASy Swiss-Prot
Match: M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 6.2e-172
Identity = 285/554 (51.44%), Postives = 374/554 (67.51%), Query Frame = 0


Query: 26  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENV 85
           TA +    Y WEV+ +   PDC + + M +NG+ PGPTI+A  GDT++V L N L+TE +
Sbjct: 15  TASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGL 74

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ+G+PW+DG  GVTQC I PGETF Y F VD+PGT+ YH HYGMQR  GLYG
Sbjct: 75  VIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYG 134

Query: 146 SIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGR 205
           S+++ + +G+ EP  YD + NL+L+DW+H+       GL+S      W+GE  S+LI GR
Sbjct: 135 SLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSS--RPMRWIGEAQSILINGR 194

Query: 206 GRFNCSVLGS-SSLASATCN-SSSPECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGH 265
           G+FNCS+    SS +  TC      +C    L V P KTYR+R++S T+L++L+F ++GH
Sbjct: 195 GQFNCSLAAQFSSTSLPTCTFKEGDQCAPQRLHVEPNKTYRIRLASSTALASLNFAVQGH 254

Query: 266 TMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGLA 325
            + VVEADG+Y+ PF  +++ IYSGETYSVL+  DQDPS+NY+IT  V  R+  TPP L 
Sbjct: 255 KLVVVEADGNYITPFTTDDIDIYSGETYSVLLTTDQDPSQNYYITAGVRGRKPNTPPALT 314

Query: 326 IFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQ 385
           + NY     S+LP + PP  P WND       S  I A  G   +PP+  D+ ++LLNTQ
Sbjct: 315 VLNYVTAPSSQLPTSPPPETPRWNDFDRSKNFSKKIFAAMG-SPSPPETFDERLILLNTQ 374

Query: 386 NTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEA 445
           N + G  +W++NNVS  +P TPYL ++K+NL   F++  PP  Y   +YDI  P  N  A
Sbjct: 375 NLIEGFTKWAINNVSLAVPGTPYLGSVKYNLRTGFNRSSPPKDYPV-DYDIMTPPRNRNA 434

Query: 446 TVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSK 505
              +      FN TVD+ILQNAN ++ N SE HPWHLHGHDFWVLGYGEGKF    D   
Sbjct: 435 KQGNVSCVFPFNVTVDVILQNANGLNANASEIHPWHLHGHDFWVLGYGEGKFKPGVDEKT 494

Query: 506 YNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKL 565
           YNL+NP ++NTV L+PYGWTALRFV DNPGVW FHCHI+ H  MGMGVVFAEG++R+ K+
Sbjct: 495 YNLKNPPLRNTVALYPYGWTALRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKV 554

Query: 566 PTSIMGCGESKRFL 578
           P   +GCG +K+FL
Sbjct: 555 PDEALGCGLTKQFL 564

[Close]

BLAST of HG10016516 vs. ExPASy Swiss-Prot
Match: Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 3.4e-170
Identity = 296/576 (51.39%), Postives = 383/576 (66.49%), Query Frame = 0


Query: 12  FILLCYFLFLLQSPTA--ESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQG 71
           F LL   L  L S  +   ++T  +KW+V+    SPD  + + M ING+ PGPTI A  G
Sbjct: 9   FFLLPLILLELSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKAG 68

Query: 72  DTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTY 131
           DTV V L N L TE V IHWHGIRQIGTPW+DGT  ++QC I PGETF Y+F VD+ GTY
Sbjct: 69  DTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGTY 128

Query: 132 LYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGN 191
            YH HYGMQR  GLYGS++V + EG+ EPF YD + NL+L+DW+HK SHE    L+S  N
Sbjct: 129 FYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS--N 188

Query: 192 GFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHP--------LTVVPGK 251
              W+GEP +LL+ GRG++NC      SLA+       P+C L          L V P K
Sbjct: 189 PLRWIGEPQTLLLNGRGQYNC------SLAARFSKPPLPQCKLRGGEQYAPQILRVRPNK 248

Query: 252 TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDP 311
            YRLRV+S T+L +LS  I GH M VVEADG+YV+PF V ++ IYSGE+YSVL K DQDP
Sbjct: 249 IYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDP 308

Query: 312 SRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKA 371
           ++NYWI+ NV  R+  TP GL + NY PN  SK P   PP AP WND     + S+ I A
Sbjct: 309 TKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFA 368

Query: 372 RQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQE 431
             G    PP+   + I+LLNTQN ++G  +W++NNVS  +P   YL ++++  ++ F  +
Sbjct: 369 LMGSPKPPPQ-NHRRIILLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYG-INAFDTK 428

Query: 432 KPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLH 491
            PP  +  K+YD+   APN  +T  + ++ L FN+T+DIILQNAN ++ + SE HPWHLH
Sbjct: 429 PPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLH 488

Query: 492 GHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHI 551
           GHDFWVLGYGEGKF   KD  K+NL+NP ++NT  + P+GWTALRFV DNPGVWAFHCHI
Sbjct: 489 GHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHI 548

Query: 552 DAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFL 578
           + H  MGMGV+FAEG+  V+K+P   + CG + + L
Sbjct: 549 EPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKML 572

[Close]

BLAST of HG10016516 vs. ExPASy TrEMBL
Match: A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 546/579 (94.30%), Postives = 558/579 (96.37%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MASLLQ +QY  +LL  FLFLLQS TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           AAGLA+PG+GFTWVGEPDSLLIQGRGRFNCSVLGSS   S TCNSSS ECVL+PLTVVPG
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLYPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR RTTPPGLAIFNYYPNHQSK PPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
           EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNANMMSPNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFDIY DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of HG10016516 vs. ExPASy TrEMBL
Match: A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)

HSP 1 Score: 1149.0 bits (2971), Expect = 0.0e+00
Identity = 546/579 (94.30%), Postives = 558/579 (96.37%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MASLLQ +QY  +LL  FLFLLQS TAESRTL YKWEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MASLLQRKQYFLLLLSSFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYDHDRNLILTDWYHKSS+EH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSNEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           AAGLA+PG+GFTWVGEPDSLLIQGRGRFNCSVLGSS   S TCNSSS ECVL+PLTVVPG
Sbjct: 181 AAGLATPGSGFTWVGEPDSLLIQGRGRFNCSVLGSS---SGTCNSSSTECVLYPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIEGH+MTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHSMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR RTTPPGLAIFNYYPNHQSK PPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
           EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVD+ILQNANMMSPNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDLILQNANMMSPNNSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFDIY DPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYNDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of HG10016516 vs. ExPASy TrEMBL
Match: A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 541/579 (93.44%), Postives = 554/579 (95.68%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MAS LQS +Y  ILL +  FLLQS TAESRTL YKWEVKSELKSPDCFKK+T+TINGRSP
Sbjct: 1   MASPLQSTRYLLILLSFVFFLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVW+PEGQ+EPFSYD DRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSHEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           A GLA+PG+GF WVGEPDSLLIQGRGRFNCS+LGSS   S TCNSSS ECVLHPLTVVPG
Sbjct: 181 ATGLATPGSGFNWVGEPDSLLIQGRGRFNCSILGSS---SGTCNSSSTECVLHPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR RTTPPGLAIFNYYPNHQS+LPPTTPPA PAWNDV PRLAQS AIK
Sbjct: 301 PSRNYWITTNVVSRSRTTPPGLAIFNYYPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHTPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
           EKPPTGYDFKNYDIFNPAPNPEATVSDAI+RLDFNSTVDIILQNANMMSPNNSETHPWHL
Sbjct: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFD+YKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDLYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI+RV KLPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIERVGKLPTSIMGCGESKRFLRP 576

[Close]

BLAST of HG10016516 vs. ExPASy TrEMBL
Match: A0A6J1GE53 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1)

HSP 1 Score: 1108.6 bits (2866), Expect = 0.0e+00
Identity = 521/579 (89.98%), Postives = 542/579 (93.61%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           MA LLQS QY  IL C+F+FL  S  AESRTL YKWEVK E++SPDCFKKLT+TING SP
Sbjct: 1   MAPLLQSTQYLLILFCFFVFLSASLVAESRTLFYKWEVKYEIRSPDCFKKLTITINGGSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGD VIVELKNSL++ENVA+HWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDNVIVELKNSLASENVAVHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDR GTYLYHAHYGMQR+DGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRAGTYLYHAHYGMQRQDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           A GLA+P  GF WVGEPDSLLIQGRGRF CS+LGSS LASATCNSSSP+CV +PLTVVPG
Sbjct: 181 ATGLATPDPGFQWVGEPDSLLIQGRGRFKCSLLGSSGLASATCNSSSPQCVAYPLTVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           KTYRLRVSSLTSLSALSFQIE HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD
Sbjct: 241 KTYRLRVSSLTSLSALSFQIESHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYW TTNVVSR RTTPPGLAIFNYYPNH+ KLPPTTPPA PAWNDVAPRLAQSHAIK
Sbjct: 301 PSRNYWATTNVVSRPRTTPPGLAIFNYYPNHRRKLPPTTPPAGPAWNDVAPRLAQSHAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPK ADKVIVLLNTQNTV GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ
Sbjct: 361 ARQGFIHPPPKIADKVIVLLNTQNTVKGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
            KPPTGYDFKNYDIFNPAPN EAT SDAIFRL+F STVDIILQNANMMSPN+S+THPWHL
Sbjct: 421 AKPPTGYDFKNYDIFNPAPNQEATESDAIFRLEFKSTVDIILQNANMMSPNSSDTHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFDIY+DPSKYNLENPIMKNTV LHPYGWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDIYRDPSKYNLENPIMKNTVALHPYGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGID+V  LPTSIMGCGESKRFLRP
Sbjct: 541 IDAHFFMGMGVVFEEGIDQVGNLPTSIMGCGESKRFLRP 579

[Close]

BLAST of HG10016516 vs. ExPASy TrEMBL
Match: A0A6J1KU77 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1)

HSP 1 Score: 1104.7 bits (2856), Expect = 0.0e+00
Identity = 520/579 (89.81%), Postives = 549/579 (94.82%), Query Frame = 0


Query: 1   MASLLQSRQYQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSP 60
           M SLLQ RQY  ILLC+F+FL +S TAESRTL Y+WEVKSELKSPDCFKKLT+TINGRSP
Sbjct: 1   MGSLLQRRQYLLILLCFFVFLWESRTAESRTLYYRWEVKSELKSPDCFKKLTLTINGRSP 60

Query: 61  GPTIYAHQGDTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQ 120
           GPTIYAHQGDTVIVELKNS  TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETF+YQ
Sbjct: 61  GPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFKYQ 120

Query: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEH 180
           FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQ+EPF+YDHDRNLILTDWYHKSSHEH
Sbjct: 121 FVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSHEH 180

Query: 181 AAGLASPGNGFTWVGEPDSLLIQGRGRFNCSVLGSSSLASATCNSSSPECVLHPLTVVPG 240
           +AGLA+PG+ F WVGEPDSLLIQGRGRFNCS LG    ASATCNSSSP+CVL+P+TVVPG
Sbjct: 181 SAGLAAPGSAFNWVGEPDSLLIQGRGRFNCS-LG----ASATCNSSSPDCVLYPITVVPG 240

Query: 241 KTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQD 300
           +TYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQV+NLFIYSGETYSVLIKADQ+
Sbjct: 241 RTYRLRVSSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVSNLFIYSGETYSVLIKADQN 300

Query: 301 PSRNYWITTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIK 360
           PSRNYWITTNVVSR  TTP GLAIFNYYPNHQ K PPTTPPA PAWN VAPRLAQSHAIK
Sbjct: 301 PSRNYWITTNVVSRNSTTPLGLAIFNYYPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIK 360

Query: 361 ARQGFIHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQ 420
           ARQGFIH PPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALK+NLLHTFSQ
Sbjct: 361 ARQGFIHPPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQ 420

Query: 421 EKPPTGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHL 480
            KPPTGYDFKNY+IF+P PNPEATVSDAI+RLDFNSTVDIILQNANMM+ N SETHPWHL
Sbjct: 421 AKPPTGYDFKNYNIFDPPPNPEATVSDAIYRLDFNSTVDIILQNANMMALNTSETHPWHL 480

Query: 481 HGHDFWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCH 540
           HGHDFWVLGYGEGKFD++KDPSKYNL NPIMKNTVPLHP+GWTALRFVADNPGVWAFHCH
Sbjct: 481 HGHDFWVLGYGEGKFDMFKDPSKYNLVNPIMKNTVPLHPFGWTALRFVADNPGVWAFHCH 540

Query: 541 IDAHFFMGMGVVFAEGIDRVQKLPTSIMGCGESKRFLRP 580
           IDAHFFMGMGVVF EGI++VQKLPTSIMGCGESKRF+RP
Sbjct: 541 IDAHFFMGMGVVFEEGIEKVQKLPTSIMGCGESKRFIRP 574

[Close]

BLAST of HG10016516 vs. TAIR 10
Match: AT4G39830.1 (Cupredoxin superfamily protein )

HSP 1 Score: 808.1 bits (2086), Expect = 4.5e-234
Identity = 382/571 (66.90%), Postives = 445/571 (77.93%), Query Frame = 0


Query: 10  YQFILLCYFLFLLQSPTAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQG 69
           +  ++LC+      S   + +   +KWEVK E KSPDCF+KL +TING+ PGPTI A QG
Sbjct: 14  FNLMVLCFIALFFSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQG 73

Query: 70  DTVIVELKNSLSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTY 129
           DT++VELKNS  TENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY
Sbjct: 74  DTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTY 133

Query: 130 LYHAHYGMQREDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGN 189
           +YH+HYGMQRE GL G I V  P  + EPF+YD+DRN +LTDWYHKS  E A GLAS   
Sbjct: 134 MYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS--I 193

Query: 190 GFTWVGEPDSLLIQGRGRFNCS--VLGSSSLASATCNSSSPECVLHPLTVVPGKTYRLRV 249
            F WVGEP SL+IQGRGRFNCS  +    SL S  CN S+ +C    LTV+PGKTYRLR+
Sbjct: 194 PFKWVGEPQSLMIQGRGRFNCSNNLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRLRI 253

Query: 250 SSLTSLSALSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWI 309
            SLT+LSALSFQIEGH +TVVEADGHYVEPF V NLF+YSGETYSVL+KADQ+P RNYWI
Sbjct: 254 GSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNYWI 313

Query: 310 TTNVVSRQRTTPPGLAIFNYYPNHQSKLPPTTPPA--APAWNDVAPRLAQSHAIKARQGF 369
           T+++VSR  TTPP  A+ NYYPNH  + PPT+  +   P WND   RLAQS AIKAR+GF
Sbjct: 314 TSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARRGF 373

Query: 370 IHAPPKAADKVIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFS-QEKPP 429
           IHA P+ +DKVIVLLNTQN VNG RRWS+NNVS++ P TPYLIALK NL + F  +   P
Sbjct: 374 IHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQNLTNAFDWRFTAP 433

Query: 430 TGYDFKNYDIFNPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHD 489
             YD +NYDIF    N  AT SD I+RL FNSTVD+ILQNAN M+ NNSETHPWHLHGHD
Sbjct: 434 ENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHPWHLHGHD 493

Query: 490 FWVLGYGEGKFDIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAH 549
           FWVLGYGEGKF+  +DP +YN  +PI KNTV + P+GWTALRF ADNPGVW+FHCHI++H
Sbjct: 494 FWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSFHCHIESH 553

Query: 550 FFMGMGVVFAEGIDRVQKLPTSIMGCGESKR 576
           FFMGMG+VF  GID+V  LP+SIMGCG++KR
Sbjct: 554 FFMGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]

BLAST of HG10016516 vs. TAIR 10
Match: AT5G21105.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 591.7 bits (1524), Expect = 6.6e-169
Identity = 280/555 (50.45%), Postives = 372/555 (67.03%), Query Frame = 0


Query: 26  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENV 85
           TA +    Y WEV+ +  SPDC +   MT+NG  PGPTI A  GDT++V L N L+TE +
Sbjct: 32  TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGL 91

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GLYG
Sbjct: 92  VIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYG 151

Query: 146 SIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGR 205
           S++V + +G+SE   YD + NL+L+DW+H++      GL+S      W+GE  S+LI GR
Sbjct: 152 SLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILINGR 211

Query: 206 GRFNCSVLG--SSSLASATCN-SSSPECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS+    S++ +   C      +C    L V P KTYR+R+SS T+L++L+  ++G
Sbjct: 212 GQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQG 271

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGL 325
           H + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R+  T   L
Sbjct: 272 HKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQAL 331

Query: 326 AIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNT 385
            I NY     SKLP + PP  P W+D       S  I +  G   +PPK   K ++LLNT
Sbjct: 332 TILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNT 391

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P P 
Sbjct: 392 QNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPN 451

Query: 446 ATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPS 505
            T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D  
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQK 565
            YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVFAEG++R+ K
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGK 571

Query: 566 LPTSIMGCGESKRFL 578
           +P   +GCG +K+FL
Sbjct: 572 VPDEALGCGLTKQFL 582

[Close]

BLAST of HG10016516 vs. TAIR 10
Match: AT5G21100.1 (Plant L-ascorbate oxidase )

HSP 1 Score: 565.5 bits (1456), Expect = 5.1e-161
Identity = 271/557 (48.65%), Postives = 358/557 (64.27%), Query Frame = 0


Query: 26  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENV 85
           +A +  +   WEV+ +   PDC + + M ING+ PGPTI A  GDTVI+ + N LSTE V
Sbjct: 18  SASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDTVIIHVVNKLSTEGV 77

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ GTPW+DG  GVTQCPI PGETF Y+F+VD+ GT+ YH HYGMQR  GLYG
Sbjct: 78  VIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFYHGHYGMQRSSGLYG 137

Query: 146 SIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGR 205
            ++V  P+   E   YD + NL+L+DW+H+S   HA  LA       W+GEP SLLI GR
Sbjct: 138 MLIVRSPK---ERLIYDGEFNLLLSDWWHQSI--HAQELALSSRPMRWIGEPQSLLINGR 197

Query: 206 GRFNCS---VLGSSSLASATCNSSSPECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS                  + +C    L V P + YRLR++S T+L++L+  ++G
Sbjct: 198 GQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQG 257

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGL 325
           H + VVEADG+YV PF VN++ +YSGETYSVL+K +  PS+ YWI+  V  R+  TP  L
Sbjct: 258 HQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQAL 317

Query: 326 AIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNT 385
            + NY    +S+ P   PP  P WND     + S  I A +G+   P K+ D+ ++LLNT
Sbjct: 318 TVINYVDATESR-PSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQ-LILLNT 377

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN      +WS+NNVS ++P TPYL ++++ L   +  + P       NYDI  P PNP 
Sbjct: 378 QNLYEDYTKWSINNVSLSVPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPN 437

Query: 446 ATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPS 505
            T    I+   F   VD+ILQNAN++    SE HPWH+HGHDFWVLGYGEGKF    D  
Sbjct: 438 TTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEK 497

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQK 565
            +NL+NP ++NTV L+P+GWTA+RFV DNPGVW FHCHI+ H  MGMGVVF EG+DR+ K
Sbjct: 498 TFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGK 557

Query: 566 --LPTSIMGCGESKRFL 578
             +P   +GCG ++++L
Sbjct: 558 MEIPDEALGCGLTRKWL 567

[Close]

BLAST of HG10016516 vs. TAIR 10
Match: AT5G21105.3 (Plant L-ascorbate oxidase )

HSP 1 Score: 526.9 bits (1356), Expect = 2.0e-149
Identity = 253/511 (49.51%), Postives = 337/511 (65.95%), Query Frame = 0


Query: 26  TAESRTLTYKWEVKSELKSPDCFKKLTMTINGRSPGPTIYAHQGDTVIVELKNSLSTENV 85
           TA +    Y WEV+ +  SPDC +   MT+NG  PGPTI A  GDT++V L N L+TE +
Sbjct: 32  TASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNLTNKLTTEGL 91

Query: 86  AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFRYQFVVDRPGTYLYHAHYGMQREDGLYG 145
            IHWHGIRQ G+PW+DG  GVTQC I PGETF Y F V++PGT+ YH HYGMQR  GLYG
Sbjct: 92  VIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYGMQRSAGLYG 151

Query: 146 SIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSHEHAAGLASPGNGFTWVGEPDSLLIQGR 205
           S++V + +G+SE   YD + NL+L+DW+H++      GL+S      W+GE  S+LI GR
Sbjct: 152 SLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGEAQSILINGR 211

Query: 206 GRFNCSVLG--SSSLASATCN-SSSPECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEG 265
           G+FNCS+    S++ +   C      +C    L V P KTYR+R+SS T+L++L+  ++G
Sbjct: 212 GQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQG 271

Query: 266 HTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQRTTPPGL 325
           H + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R+  T   L
Sbjct: 272 HKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQAL 331

Query: 326 AIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNT 385
            I NY     SKLP + PP  P W+D       S  I +  G   +PPK   K ++LLNT
Sbjct: 332 TILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNT 391

Query: 386 QNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPE 445
           QN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI NP P P 
Sbjct: 392 QNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPN 451

Query: 446 ATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPS 505
            T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF    D  
Sbjct: 452 TTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEK 511

Query: 506 KYNLENPIMKNTVPLHPYGWTALRFVADNPG 534
            YNL+NP ++NT  L+PYGWTA+RFV DNPG
Sbjct: 512 TYNLKNPPLRNTAILYPYGWTAIRFVTDNPG 538

[Close]

BLAST of HG10016516 vs. TAIR 10
Match: AT5G21105.2 (Plant L-ascorbate oxidase )

HSP 1 Score: 390.2 bits (1001), Expect = 2.9e-108
Identity = 190/382 (49.74%), Postives = 255/382 (66.75%), Query Frame = 0


Query: 199 SLLIQGRGRFNCSVLG--SSSLASATCN-SSSPECVLHPLTVVPGKTYRLRVSSLTSLSA 258
           S+LI GRG+FNCS+    S++ +   C      +C    L V P KTYR+R+SS T+L++
Sbjct: 12  SILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNKTYRIRLSSTTALAS 71

Query: 259 LSFQIEGHTMTVVEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRQ 318
           L+  ++GH + VVEADG+Y+ PF  +++ IYSGE+YSVL+  DQDPS+NY+I+  V  R+
Sbjct: 72  LNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDPSQNYYISVGVRGRK 131

Query: 319 RTTPPGLAIFNYYPNHQSKLPPTTPPAAPAWNDVAPRLAQSHAIKARQGFIHAPPKAADK 378
             T   L I NY     SKLP + PP  P W+D       S  I +  G   +PPK   K
Sbjct: 132 PNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFSAMG-SPSPPKKYRK 191

Query: 379 VIVLLNTQNTVNGRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIF 438
            ++LLNTQN ++G  +W++NNVS   P TPYL ++K+NL   F+++ PP  Y   +YDI 
Sbjct: 192 RLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIM 251

Query: 439 NPAPNPEATVSDAIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKF 498
           NP P P  T  + I+   FN TVD+I+QNAN++    SE HPWHLHGHDFWVLGYG+GKF
Sbjct: 252 NPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIHPWHLHGHDFWVLGYGDGKF 311

Query: 499 DIYKDPSKYNLENPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAE 558
               D   YNL+NP ++NT  L+PYGWTA+RFV DNPGVW FHCHI+ H  MGMGVVFAE
Sbjct: 312 KPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAE 371

Query: 559 GIDRVQKLPTSIMGCGESKRFL 578
           G++R+ K+P   +GCG +K+FL
Sbjct: 372 GLNRIGKVPDEALGCGLTKQFL 391

The following BLAST results are available for this feature:

BLAST of HG10016516 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038881120.1	0.0e+00	95.34	L-ascorbate oxidase [Benincasa hispida]	[more]
XP_008440636.1	0.0e+00	94.30	PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase ...	[more]
XP_004143482.1	0.0e+00	93.44	L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_00307...	[more]
KAG6594863.1	0.0e+00	90.16	L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826...	[more]
XP_022949916.1	0.0e+00	89.98	L-ascorbate oxidase-like [Cucurbita moschata] >XP_022949917.1 L-ascorbate oxidas...	[more]

BLAST of HG10016516 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P14133	1.2e-175	53.60	L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1	[more]
P24792	1.1e-173	50.60	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2	[more]
P37064	5.6e-173	51.74	L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1	[more]
M4DUF2	6.2e-172	51.44	L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1	[more]
Q40588	3.4e-170	51.39	L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1	[more]

BLAST of HG10016516 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SYW4	0.0e+00	94.30	L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G...	[more]
A0A1S3B151	0.0e+00	94.30	L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1	[more]
A0A0A0KK62	0.0e+00	93.44	L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1	[more]
A0A6J1GE53	0.0e+00	89.98	L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1	[more]
A0A6J1KU77	0.0e+00	89.81	L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=LOC111497239 PE=3 SV=1	[more]

BLAST of HG10016516 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G39830.1	4.5e-234	66.90	Cupredoxin superfamily protein	[more]
AT5G21105.1	6.6e-169	50.45	Plant L-ascorbate oxidase	[more]
AT5G21100.1	5.1e-161	48.65	Plant L-ascorbate oxidase	[more]
AT5G21105.3	2.0e-149	49.51	Plant L-ascorbate oxidase	[more]
AT5G21105.2	2.9e-108	49.74	Plant L-ascorbate oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011707	Multicopper oxidase, N-termianl	PFAM	PF07732	Cu-oxidase_3	coord: 41..150 e-value: 1.1E-38 score: 131.8
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 167..329 e-value: 3.7E-47 score: 160.4
IPR011706	Multicopper oxidase, C-terminal	PFAM	PF07731	Cu-oxidase_2	coord: 433..556 e-value: 1.2E-37 score: 128.7
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 159..365 e-value: 4.5E-63 score: 215.0
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 20..152 e-value: 1.1E-47 score: 164.0
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 367..577 e-value: 6.9E-70 score: 237.0
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 19..178
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 370..572
IPR008972	Cupredoxin	SUPERFAMILY	49503	Cupredoxins	coord: 158..350
IPR017760	L-ascorbate oxidase, plants	TIGRFAM	TIGR03388	TIGR03388	coord: 33..572 e-value: 6.2E-284 score: 940.6
None	No IPR available	PANTHER	PTHR11709:SF237	CUPREDOXIN SUPERFAMILY PROTEIN	coord: 10..574
IPR045087	Multicopper oxidase	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 10..574
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 538..549
IPR033138	Multicopper oxidases, conserved site	PROSITE	PS00079	MULTICOPPER_OXIDASE1	coord: 533..553
IPR034267	Ascorbate oxidase, third cupredoxin domain	CDD	cd13893	CuRO_3_AAO	coord: 373..564 e-value: 2.61207E-84 score: 257.734

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
HG10016516.1	HG10016516.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900367	positive regulation of defense response to insect
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0008447	L-ascorbate oxidase activity
molecular_function	GO:0016491	oxidoreductase activity