HG10016377 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016377
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationChr03: 4615986 .. 4620011 (-)
RNA-Seq ExpressionHG10016377
SyntenyHG10016377
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAATTAGTCATCAGCATCTCAAACTTGAAGACCCTTTTCTTCATAATTACAAGCCCTCAGAGCTACGGATCGCTTCTGAGTTTCTCACCACTTGGCTTCCGTTTCTTTCGAGAGATCTCTGCGAAGACTGCACCAAATTGCTCTCGAATCGTATCCGCGCCCTCGATCCAGGTATTCTTTTGTTTTTTTTTTATTCGTTCTCGATGAATTATAGCACGAGGGGCGTTAGAGAGAATAACCCATCTAATTTTCTAGGTTCTTTAAAATAATTGGGTGTACGAGGGGAAAATATTAAAAAAATTTGAAGAGTTGGGGAATTGATTTTATTAAAGTAGAGTTCTTGAGATTTCTTGATGTGTTGTGTTGCTAAAAAGAGTGAGGCGTCTGCTGTGTTTGATAATTTACATGATCTGTTCCTTTGGTTTGCAAGACCTCGATGCGTCCCCCGTTTAATCATTAGGATTATCTGATGAAATTGTTGAACTTGACAATGTTGTAGCTGTCTGTTCCGACGAGAATTTAAGTTCTGCATCACCAGTGGAAGACATGCATGAGAGTAATGGGAATCAGGATGATGCTTGTGATGCAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACAAATTCATTAGGGAGTTGGAAGGACGGCATAACTGAAGGGAATGAAGCTGATGGAGTACCTGAAACTTCTTCTAGTGAACTGTCCTCTAAATTAAATTCCACCAAGACCTCAGGGCCTCGAATGTCGTGGGCTGATATGACTCAGGAAGATGAACTAGAAGAAGAGGAAGATGAGTACGAATTAGAAAAAAGATTAGTCAGTATTAATGAGTCCACAAGGAAATTAACAATATCGAAGGTTATTGAGAAGCCACAGCTGTCTAGAGAGCAGAGAGAGTACATCAGATTCATGAATGTTGGACGGAAGAAAGATTTTATTTGTCTGGAAAGGTTTAAAGGGAAAGTAGTAAATATTCTTGAAGGACTCGAGCTTCATACTGGTATTTTTAGTGCTGCAGAACAGAAGAGAATAGTTGATCATGTTTATGCACTTCAGGAAATGGGCAAGAAGGGAGAATTAAGAGGTAATTATTCCCGTTTGCATCTTGATTTCCTTTTATCTTCTACTGAATGCTGAAATAGTAGTTCATGTGTAACATAGGGACTCGTCCAATTCCTGATTAGTTTTTTTTTGCTTAGTATGTTATGTCTGATGTACCTGGGGCCTGGGCTCATCTAATATTTTGCATCAGTATAAGTACAGTCTAGTATCTATAAAGCTGTTAGCCAATGAAAAGTGCAACATAAGGACCCGTCCTATTTCCATTTAGTCTTTTTTGTAGTAGAGTGTAGGGTAATAAGCTAATATCATGTTGGATAAAACAAAGGGTTCCAATATCTTATAGTCCAGGTTCTAAATGCTAATGCTACTCTCTAATTAACCACACCACAGATTGACTCATTCCAATATAAAATACTTTATTTATAAACTACTGGAGGTTTAAGTTTGTAACTCTTATTTCGATTCCCCCACCTCACATGTTGTAAAAAAAAATATATTGGGGGTTTGTTTTTTGCTAGTACCTCTCTTGGATTTATATCTTTAAATCCATTCATTCATCTTGGTTGTTAACCTTCTTATGGGTGCTGGATCTTTTTGCTACCAATATAACAAGTTTCTTGACAATTTAGGGGAAACGACTGAGAATTGTGACAATAGCTAACCGGTTGCTGCTTTTAGTTTTGTTAAGTGGAATTAGAACAACTCTGTTAGGCCTCCAATTACTAAAATCTATAGGAGGGGTAAGTTGGGTAATCACCCCAGACAGGGGAGGAGCAGGAAGTGTTTGTTAGTTGACGACTGTCAGAGGGGGGGGGGGGGGGGGGGTGTATTTTAGCTTTTGTTGAGCAAGGTAGTGAGTATTCTGTATTTTTGGCAATAAAATAGTAGTTCTTGTGGGAGAGATTACCAACCCTCTCGAATAGGCTGGGTGGTTCTTGTTATTTTCCCTTATAATCCTGTTCTCAGAAGTTAGTAATACAATATAGTAAGGAGGTACCCGTACAAATTCTTCCTGCCAAATTACGAAGCAAATTTGAACACATATTCATTTTCTCTGCTCTCTTTGGTTGATTGTATGTCAAATTCACAGACTTCATTGTTTATGGTGCACATTGTTTAATCTGTGTATGGATGTAGTCCAGAGAGAGCAATATTCGTTAACTGATAAAAAAAATTGTCAAATACGGTGGCTATATCCTTGAAGATGTGATGGTTGCACTCGAACAAAAGAAAGACTTTGATGAAGCAATTCCAAAGGACTTACTTTTTCTTGCTAAAAGAGTGATCAATGAGGATCCATTGAAGTAAAGTCATTAGCTTCCCCATTAGAGCTAGGTGCCAATTAAATGCCAATAGGAAATGATTTCAAAATAAAGCAGCATAAGGACAAGTAATGAAGAAATCTAGCAAGAATTCATTGTCCTTTTGACGAAGTACTCACCAGTTTGGTGAGGAACCAATTCAGGGTCCCTTATCTTTTTCTTTTTTTTCCTTTTTTTTTTCTTGTTTGGAACATCAACAGTGCAAATGCTTCTATGACTAAGTTCCCACAAGAAAATTTCTGATTTTCTTTGGTACCCTTCCACAAACGAGAGTGTGTATTGCTGTATGTTGGTGTGATGTTGAGGATGTGATTCTTTGAGTAAGGGATTTTGATGTGAAGAGTCCATTTTTATCCAATGGCCATCTCCAACTTTTTTTATTTTTCTGTTCTGTACAAACATGACAATGGCTTTCTTCCCCTTGTTTTTTGTTTTTTTGTTTTTTTTTCTTCTTTTGATTGAAAACATGGCGATGACTTGAATGCAACACCACATTCACCATTTTACATTTTTTTGCAGAACGAACATTTTCAGCTCCTAAAAAGTGGATGAAGGGAAAGGGGCGTGTAACTATGCAGTTTGGGTGCTGTTACAATTATGCACCTGTGAGTCTATCTGATTGCTAAGTTGGTGTGGTTCTTTTGAATTGCATTTAATTTTTCACTTTTGAGGATCAACCAGTTGTGATGCTCGGCTAGTATCCTTAATTAGAATATCATTTTTAATGAAGGTAAATCATTCTAATGACAGGATAAAAATGGCAATCCTCCTGGAATTCTTCGAAGTGAAATTGTGGATCCAATACCTTCTCTCTTTAAGGTGATCATTAGAAGGTTGGTGAAGTGGCATGTGCTTCCTCCAACATGTGTTCCAGATAGTTGCATTGTGAACATCTACGATGAAGGGGACTGTATTCCTCCTCATATTGACAACCACGATTTTGTTCGACCATTTTGCACTGTGTCATTTCTCAGTGAATGCAATATTGTTTTTGGATCAAACCTTTCTATTGTAGGACCGGGTGAATTTTCTGGGCCAATTGCAATCCCTCTGCCTGTGGGGTATGTATATGATAATGATATGCAGTCGACTATGTTGACTTTTGTGGTTAATTACCCTTTTCCATCTCTGCAGACTGTCAATTGCATTAGATTTTCAATCTTACTGTTATTGATTTTCTCGCAGGTCCGTACTTGTATTAAATGGAAATGGAGCTGATGTTGCTAAGCATTGTGTACCTGCAGTCCCTACAAAGAGGTATAATTCAGGAAGGTCATTTGTTTACGGTTCTATTTAGCCACGATCTTTACTTGCTAGTTATTCAACCTAACTTGCCTCTTATCTGGTCTATTTAGGATATCAATTACATTTAGAAGAATTGATGAATCCAAAAGGCCAATTGAGTATGCTTCAGAACCGGATTTGCAGGGAATTCAACCATTGCCTTACGAAGCAAGTGAAAATGATGTACCAACTTCTCCTGTATCATCAGAAAGGGAAATAAGGAGGCAGCCATTTAGGAGGGGTAGTCATATGAGAACCAGAGGATCTGGAAACAGAAGCGACACTCGATTTGATCCACGCAATCCAGGTAGAGCTCGACATAATTCAGCAGATAGGAGAAGCAGAGAGTAA

mRNA sequence

ATGGCAATTAGTCATCAGCATCTCAAACTTGAAGACCCTTTTCTTCATAATTACAAGCCCTCAGAGCTACGGATCGCTTCTGAGTTTCTCACCACTTGGCTTCCGTTTCTTTCGAGAGATCTCTGCGAAGACTGCACCAAATTGCTCTCGAATCGTATCCGCGCCCTCGATCCAGGATTATCTGATGAAATTGTTGAACTTGACAATGTTGTAGCTGTCTGTTCCGACGAGAATTTAAGTTCTGCATCACCAGTGGAAGACATGCATGAGAGTAATGGGAATCAGGATGATGCTTGTGATGCAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACAAATTCATTAGGGAGTTGGAAGGACGGCATAACTGAAGGGAATGAAGCTGATGGAGTACCTGAAACTTCTTCTAGTGAACTGTCCTCTAAATTAAATTCCACCAAGACCTCAGGGCCTCGAATGTCGTGGGCTGATATGACTCAGGAAGATGAACTAGAAGAAGAGGAAGATGAGTACGAATTAGAAAAAAGATTAGTCAGTATTAATGAGTCCACAAGGAAATTAACAATATCGAAGGTTATTGAGAAGCCACAGCTGTCTAGAGAGCAGAGAGAGTACATCAGATTCATGAATGTTGGACGGAAGAAAGATTTTATTTGTCTGGAAAGGTTTAAAGGGAAAGTAGTAAATATTCTTGAAGGACTCGAGCTTCATACTGGTATTTTTAGTGCTGCAGAACAGAAGAGAATAGTTGATCATGTTTATGCACTTCAGGAAATGGGCAAGAAGGGAGAATTAAGAGAACGAACATTTTCAGCTCCTAAAAAGTGGATGAAGGGAAAGGGGCGTGTAACTATGCAGTTTGGGTGCTGTTACAATTATGCACCTGATAAAAATGGCAATCCTCCTGGAATTCTTCGAAGTGAAATTGTGGATCCAATACCTTCTCTCTTTAAGGTGATCATTAGAAGGTTGGTGAAGTGGCATGTGCTTCCTCCAACATGTGTTCCAGATAGTTGCATTGTGAACATCTACGATGAAGGGGACTGTATTCCTCCTCATATTGACAACCACGATTTTGTTCGACCATTTTGCACTGTGTCATTTCTCAGTGAATGCAATATTGTTTTTGGATCAAACCTTTCTATTGTAGGACCGGGTGAATTTTCTGGGCCAATTGCAATCCCTCTGCCTGTGGGGTCCGTACTTGTATTAAATGGAAATGGAGCTGATGTTGCTAAGCATTGTGTACCTGCAGTCCCTACAAAGAGGATATCAATTACATTTAGAAGAATTGATGAATCCAAAAGGCCAATTGAGTATGCTTCAGAACCGGATTTGCAGGGAATTCAACCATTGCCTTACGAAGCAAGTGAAAATGATGTACCAACTTCTCCTGTATCATCAGAAAGGGAAATAAGGAGGCAGCCATTTAGGAGGGGTAGTCATATGAGAACCAGAGGATCTGGAAACAGAAGCGACACTCGATTTGATCCACGCAATCCAGGTAGAGCTCGACATAATTCAGCAGATAGGAGAAGCAGAGAGTAA

Coding sequence (CDS)

ATGGCAATTAGTCATCAGCATCTCAAACTTGAAGACCCTTTTCTTCATAATTACAAGCCCTCAGAGCTACGGATCGCTTCTGAGTTTCTCACCACTTGGCTTCCGTTTCTTTCGAGAGATCTCTGCGAAGACTGCACCAAATTGCTCTCGAATCGTATCCGCGCCCTCGATCCAGGATTATCTGATGAAATTGTTGAACTTGACAATGTTGTAGCTGTCTGTTCCGACGAGAATTTAAGTTCTGCATCACCAGTGGAAGACATGCATGAGAGTAATGGGAATCAGGATGATGCTTGTGATGCAAATTCTCTTGGAAGTTGGAAAGATGAAGCGGAAACAAATTCATTAGGGAGTTGGAAGGACGGCATAACTGAAGGGAATGAAGCTGATGGAGTACCTGAAACTTCTTCTAGTGAACTGTCCTCTAAATTAAATTCCACCAAGACCTCAGGGCCTCGAATGTCGTGGGCTGATATGACTCAGGAAGATGAACTAGAAGAAGAGGAAGATGAGTACGAATTAGAAAAAAGATTAGTCAGTATTAATGAGTCCACAAGGAAATTAACAATATCGAAGGTTATTGAGAAGCCACAGCTGTCTAGAGAGCAGAGAGAGTACATCAGATTCATGAATGTTGGACGGAAGAAAGATTTTATTTGTCTGGAAAGGTTTAAAGGGAAAGTAGTAAATATTCTTGAAGGACTCGAGCTTCATACTGGTATTTTTAGTGCTGCAGAACAGAAGAGAATAGTTGATCATGTTTATGCACTTCAGGAAATGGGCAAGAAGGGAGAATTAAGAGAACGAACATTTTCAGCTCCTAAAAAGTGGATGAAGGGAAAGGGGCGTGTAACTATGCAGTTTGGGTGCTGTTACAATTATGCACCTGATAAAAATGGCAATCCTCCTGGAATTCTTCGAAGTGAAATTGTGGATCCAATACCTTCTCTCTTTAAGGTGATCATTAGAAGGTTGGTGAAGTGGCATGTGCTTCCTCCAACATGTGTTCCAGATAGTTGCATTGTGAACATCTACGATGAAGGGGACTGTATTCCTCCTCATATTGACAACCACGATTTTGTTCGACCATTTTGCACTGTGTCATTTCTCAGTGAATGCAATATTGTTTTTGGATCAAACCTTTCTATTGTAGGACCGGGTGAATTTTCTGGGCCAATTGCAATCCCTCTGCCTGTGGGGTCCGTACTTGTATTAAATGGAAATGGAGCTGATGTTGCTAAGCATTGTGTACCTGCAGTCCCTACAAAGAGGATATCAATTACATTTAGAAGAATTGATGAATCCAAAAGGCCAATTGAGTATGCTTCAGAACCGGATTTGCAGGGAATTCAACCATTGCCTTACGAAGCAAGTGAAAATGATGTACCAACTTCTCCTGTATCATCAGAAAGGGAAATAAGGAGGCAGCCATTTAGGAGGGGTAGTCATATGAGAACCAGAGGATCTGGAAACAGAAGCGACACTCGATTTGATCCACGCAATCCAGGTAGAGCTCGACATAATTCAGCAGATAGGAGAAGCAGAGAGTAA

Protein sequence

MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGLSDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVSINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE
Homology
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BLAST of HG10016377 vs. NCBI nr
Match: XP_038882153.1 (RNA demethylase ALKBH9B [Benincasa hispida])

HSP 1 Score: 953.7 bits (2464), Expect = 6.3e-274
Identity = 480/516 (93.02%), Postives = 489/516 (94.77%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC DCTKLLS+RIRALDP  
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRDCTKLLSDRIRALDP-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      AV S EN  +A PVEDMHESNGNQDDACDANSLGSWKDE ETNSLGSWK
Sbjct: 61  -----------AVRSGENFGAAPPVEDMHESNGNQDDACDANSLGSWKDEGETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVS 180
           DGI EGNEAD VPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEE+DEYE EKRLVS
Sbjct: 121 DGINEGNEADVVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEDDEYESEKRLVS 180

Query: 181 INESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTG 240
           INESTRKLTISKVIEKP LSREQRE+IRFMNVGRKKDFICLERFKGK+VNILEGLELHTG
Sbjct: 181 INESTRKLTISKVIEKPTLSREQREHIRFMNVGRKKDFICLERFKGKLVNILEGLELHTG 240

Query: 241 IFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG 300
           IFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDKNG
Sbjct: 241 IFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKNG 300

Query: 301 NPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF 360
           NPPGILRSEIVDPIPSLFKVIIRRLV+WHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF
Sbjct: 301 NPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF 360

Query: 361 VRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV 420
           VRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV
Sbjct: 361 VRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV 420

Query: 421 PTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRR 480
           PTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRR
Sbjct: 421 PTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRR 480

Query: 481 GSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           G HMR RGSGNRSDTRFDPRNPGR RH+SADRR+RE
Sbjct: 481 GGHMRNRGSGNRSDTRFDPRNPGRGRHSSADRRTRE 503

[Close]
BLAST of HG10016377 vs. NCBI nr
Match: KAG7034097.1 (RNA demethylase ALKBH5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 944.9 bits (2441), Expect = 2.9e-271
Identity = 474/517 (91.68%), Postives = 495/517 (95.74%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDPGL
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDPGL 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
           SD I ELDNV A   DEN SS+ PVEDMHESNGNQ+D CDANSLGSWKDEAETNSLGSWK
Sbjct: 61  SDGIFELDNVAAARFDENSSSSPPVEDMHESNGNQNDGCDANSLGSWKDEAETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDEYELEKRLV 180
           DGI EGNEADGVPETSSS  SSK+ STKTSGPRMSWADMTQEDEL EEEEDEYE EKR+V
Sbjct: 121 DGINEGNEADGVPETSSSVPSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVV 180

Query: 181 SINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHT 240
           S+NESTRKLTISKVIEKP+LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELHT
Sbjct: 181 SVNESTRKLTISKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHT 240

Query: 241 GIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKN 300
           GIFSAAEQKRIVDHVY+LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDKN
Sbjct: 241 GIFSAAEQKRIVDHVYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKN 300

Query: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHD 360
           GNPPGILRSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIYDEGDCIPPHID+HD
Sbjct: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSHD 360

Query: 361 FVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420
           FVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA
Sbjct: 361 FVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420

Query: 421 VPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFR 480
           VPTKRISITFRR+D SK PIEYA EPDLQGIQPLPYEASEN+VP SPVSSEREIRRQPFR
Sbjct: 421 VPTKRISITFRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPRSPVSSEREIRRQPFR 480

Query: 481 RGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RGSHMRTRGSGNR+DTRFD RNPGR RH+SADRRSRE
Sbjct: 481 RGSHMRTRGSGNRTDTRFDSRNPGRGRHSSADRRSRE 517

[Close]
BLAST of HG10016377 vs. NCBI nr
Match: XP_023544551.1 (uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544552.1 uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544553.1 uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 918.7 bits (2373), Expect = 2.2e-263
Identity = 464/517 (89.75%), Postives = 486/517 (94.00%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDP  
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  +DEN SS  PVE MHESNGNQ+DACDANSLGSWKDEAETNSLGSWK
Sbjct: 61  -----------AARTDENSSSTPPVEVMHESNGNQNDACDANSLGSWKDEAETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDEYELEKRLV 180
           DGI EGNEADGVPETSSS  SSK+ STKTSGPRMSWADMTQEDEL EEEEDEYE EKR+V
Sbjct: 121 DGINEGNEADGVPETSSSIPSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVV 180

Query: 181 SINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHT 240
           S+NESTRKLTISKVIEKP+LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELHT
Sbjct: 181 SVNESTRKLTISKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHT 240

Query: 241 GIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKN 300
           GIFSAAEQKRIVDH+Y+LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDKN
Sbjct: 241 GIFSAAEQKRIVDHIYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKN 300

Query: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHD 360
           GNPPGILRSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIYDEGDCIPPHID+HD
Sbjct: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSHD 360

Query: 361 FVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420
           FVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA
Sbjct: 361 FVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420

Query: 421 VPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFR 480
           VPTKRISITFRR+D SK PIEYA EPDLQGIQPLPYEASEN+VPTSPVSSEREIRRQPFR
Sbjct: 421 VPTKRISITFRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFR 480

Query: 481 RGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RGSHMRTRGSGNR+DTRFD RNPGR RH+SADRRSRE
Sbjct: 481 RGSHMRTRGSGNRTDTRFDSRNPGRGRHSSADRRSRE 504

[Close]
BLAST of HG10016377 vs. NCBI nr
Match: XP_022979042.1 (RNA demethylase ALKBH5 [Cucurbita maxima] >XP_022979043.1 RNA demethylase ALKBH5 [Cucurbita maxima] >XP_022979044.1 RNA demethylase ALKBH5 [Cucurbita maxima] >XP_022979045.1 RNA demethylase ALKBH5 [Cucurbita maxima])

HSP 1 Score: 909.8 bits (2350), Expect = 1.0e-260
Identity = 462/518 (89.19%), Postives = 483/518 (93.24%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDP  
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  +DEN SS  PVE MHESNGNQ+DACDANSLGSWKDEAETNSLGSWK
Sbjct: 61  -----------ASRTDENSSSTPPVEVMHESNGNQNDACDANSLGSWKDEAETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEE--DEYELEKRL 180
           DGI EGNEADGVPETSSS  S+K+ S KTSGPRMSWADMTQEDELEEEE  DEYE EKR+
Sbjct: 121 DGINEGNEADGVPETSSSVPSTKVVSIKTSGPRMSWADMTQEDELEEEEEDDEYESEKRV 180

Query: 181 VSINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELH 240
           VS+NESTRKLTISKVIEKP+LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELH
Sbjct: 181 VSVNESTRKLTISKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELH 240

Query: 241 TGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDK 300
           TGIFSAAEQKRIVDHVY LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDK
Sbjct: 241 TGIFSAAEQKRIVDHVYLLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDK 300

Query: 301 NGNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNH 360
           NGNPPGILRSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIYDEGDCIPPHID+H
Sbjct: 301 NGNPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSH 360

Query: 361 DFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVP 420
           DFVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVP
Sbjct: 361 DFVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVP 420

Query: 421 AVPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPF 480
           AVPTKRISITFRR+DESK PIEYA EPDLQGIQPLPYEASEN+VPTSPVSSEREIRRQ F
Sbjct: 421 AVPTKRISITFRRMDESKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQSF 480

Query: 481 RRGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RRGSHMRTRGSGNR+DTRFD RN GR RH+SADRRSRE
Sbjct: 481 RRGSHMRTRGSGNRTDTRFDSRNLGRGRHSSADRRSRE 505

[Close]
BLAST of HG10016377 vs. NCBI nr
Match: XP_022950629.1 (uncharacterized protein LOC111453671 isoform X1 [Cucurbita moschata])

HSP 1 Score: 907.9 bits (2345), Expect = 4.0e-260
Identity = 460/517 (88.97%), Postives = 483/517 (93.42%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDP  
Sbjct: 40  MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP-- 99

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  +DEN SS  PVE MHESNGNQ+DACDANSLGSWKDEAETNSL SWK
Sbjct: 100 -----------AARTDENSSSTPPVEVMHESNGNQNDACDANSLGSWKDEAETNSLESWK 159

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDEYELEKRLV 180
           DGI EGNEADGVPETSSS  SSK+ STKTSGPRMSWADMTQEDEL EEEEDEYE EKR+V
Sbjct: 160 DGINEGNEADGVPETSSSVQSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVV 219

Query: 181 SINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHT 240
           S+NESTRKLTISKVIEK +LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELHT
Sbjct: 220 SVNESTRKLTISKVIEKAKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHT 279

Query: 241 GIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKN 300
           GIFSAAEQKRIVDHVY+LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDKN
Sbjct: 280 GIFSAAEQKRIVDHVYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKN 339

Query: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHD 360
           GNPPGI+RSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIY+EGDCIPPHID+HD
Sbjct: 340 GNPPGIIRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYNEGDCIPPHIDSHD 399

Query: 361 FVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420
           FVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA
Sbjct: 400 FVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 459

Query: 421 VPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFR 480
           VPTKRISITFRR+D SK PIEYA EPDLQGIQPLPYEASEN+VPTSPVSSEREIRRQPFR
Sbjct: 460 VPTKRISITFRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFR 519

Query: 481 RGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RGSHMRTRGSGNR+DTRFD RN GR RH+SADRRSRE
Sbjct: 520 RGSHMRTRGSGNRTDTRFDSRNSGRGRHSSADRRSRE 543

[Close]
BLAST of HG10016377 vs. ExPASy Swiss-Prot
Match: Q9SL49 (RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 2.2e-160
Identity = 307/510 (60.20%), Postives = 371/510 (72.75%), Query Frame = 0 

Query: 12  DPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGLSDEIVELDNVV 71
           DPFL  Y+PSEL+IASEFLT WLPFLS+DLC+DC  LLSNRIR+LDP         +N  
Sbjct: 4   DPFLRQYQPSELKIASEFLTNWLPFLSKDLCKDCNHLLSNRIRSLDPAHCS-----NNTD 63

Query: 72  AVCSDENLSSASPVEDM--HESNGNQDDACDANSLGSWKDEAETNSLGSWKDGITEGNEA 131
            V  +    S S VE+M    ++ N DD  D  S     ++ + +SLGSWK     G+E 
Sbjct: 64  KVDGECKTGSCSVVENMGSERASNNVDDNYDEKSENG--EDCDNHSLGSWK-----GSEI 123

Query: 132 --DGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVSINESTRK 191
                PE  SS L S+    +T+ PRM WADM QEDE +EEE+E E E+      +S+RK
Sbjct: 124 VFGSFPEDFSSVLQSRPAVVETASPRMRWADMAQEDEFDEEEEEEEEER------DSSRK 183

Query: 192 ---LTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTGIFSA 251
               +  K  EKP+LSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA
Sbjct: 184 GFDASSMKTPEKPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSA 243

Query: 252 AEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNGNPPG 311
            EQKRIVD VY LQE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPG
Sbjct: 244 VEQKRIVDQVYQLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPG 303

Query: 312 ILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPF 371
           IL+ E VDP+P LFKVIIR+L+KWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF+RPF
Sbjct: 304 ILQREEVDPLPHLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPF 363

Query: 372 CTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKR 431
           CT+SFLSEC+I+FGSNL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKR
Sbjct: 364 CTISFLSECDILFGSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKR 423

Query: 432 ISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGSHM 491
           ISITFR++DESKRP+ +  EPDLQGI+PLP + + +        S     R     G++ 
Sbjct: 424 ISITFRKMDESKRPVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQ 483

Query: 492 R---TRGSGNRSDTRFDPRNPGR-ARHNSA 511
           R    RG GN  D+R    NP R + HN +
Sbjct: 484 RGHGRRGGGNGYDSR-GYYNPERSSEHNDS 487

[Close]
BLAST of HG10016377 vs. ExPASy Swiss-Prot
Match: Q9ZT92 (RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 3.4e-28
Identity = 74/219 (33.79%), Postives = 116/219 (52.97%), Query Frame = 0 

Query: 216 KDFICLERFKGKVVNILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPK 275
           K F   E+ KG  VN+++GL+L+  +    E  +++D V  L+E G  G+L   +F    
Sbjct: 223 KGFQAKEQVKGHTVNVVKGLKLYEELLKEDEISKLLDFVAELREAGINGKLAGESFILFN 282

Query: 276 KWMKGKGRVTMQFGCCY--NYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPP 335
           K +KG  R  +Q G     +   D+N N      S  ++PIP L + +I   V W ++P 
Sbjct: 283 KQIKGNKRELIQLGVPIFGHVKADENSNDTN--NSVNIEPIPPLLESVIDHFVTWRLIPE 342

Query: 336 TCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPI 395
              P+ C++N ++EG+   P +      +P  T+  LSE  + +G  LS    G F GP+
Sbjct: 343 YKRPNGCVINFFEEGEYSQPFLKPPHLEQPISTL-VLSESTMAYGRILSSDNEGNFRGPL 402

Query: 396 AIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRI 433
            + L  GS+LV+ GN AD+A+H +     KR+SITF RI
Sbjct: 403 TLSLKQGSLLVMRGNSADMARHVMCPSQNKRVSITFFRI 438

[Close]
BLAST of HG10016377 vs. ExPASy Swiss-Prot
Match: Q66JG8 (RNA demethylase ALKBH5 OS=Xenopus tropicalis OX=8364 GN=alkbh5 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.0e-12
Identity = 67/208 (32.21%), Postives = 98/208 (47.12%), Query Frame = 0 

Query: 241 IFSAAEQKRIVDHVYALQEMGKKGELRERTFS-AP--KKWMKGKGRVTMQFGCCYNYAPD 300
           +FS  E  RI   +  +    +KG  RE T   AP   K+  G+G         Y Y   
Sbjct: 60  LFSPDECARIEAKIDEVVSRAEKGLYREHTVDRAPLRNKYFFGEG---------YTYGAQ 119

Query: 301 KNGNPPG---ILRSEIVDPIPS-LFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPP 360
                PG   +     VD IP+ + +++IRRLV+  V+P   V +S ++N Y  G CI  
Sbjct: 120 LQRRGPGQERLYPKGEVDEIPAWVNELVIRRLVEHRVIPEGFV-NSAVINDYQPGGCIVS 179

Query: 361 HIDN-HDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPI-AIPLPVGSVLVLNGNGAD 420
           H+D  H F RP  +VSF S+  + FG       P   S P+  +P+  GSV VL+G  AD
Sbjct: 180 HVDPIHIFERPIVSVSFFSDSALCFGCKFQF-KPIRVSEPVFFLPVQRGSVTVLSGYAAD 239

Query: 421 VAKHCV-PAVPTKRISITFRRIDESKRP 439
              HC+ P    +R ++   R   ++ P
Sbjct: 240 EITHCIRPQDIKERRAVVILRKTRTEAP 256

[Close]
BLAST of HG10016377 vs. ExPASy TrEMBL
Match: A0A6J1IMQ3 (RNA demethylase ALKBH5 OS=Cucurbita maxima OX=3661 GN=LOC111478802 PE=4 SV=1)

HSP 1 Score: 909.8 bits (2350), Expect = 5.1e-261
Identity = 462/518 (89.19%), Postives = 483/518 (93.24%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDP  
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  +DEN SS  PVE MHESNGNQ+DACDANSLGSWKDEAETNSLGSWK
Sbjct: 61  -----------ASRTDENSSSTPPVEVMHESNGNQNDACDANSLGSWKDEAETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEE--DEYELEKRL 180
           DGI EGNEADGVPETSSS  S+K+ S KTSGPRMSWADMTQEDELEEEE  DEYE EKR+
Sbjct: 121 DGINEGNEADGVPETSSSVPSTKVVSIKTSGPRMSWADMTQEDELEEEEEDDEYESEKRV 180

Query: 181 VSINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELH 240
           VS+NESTRKLTISKVIEKP+LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELH
Sbjct: 181 VSVNESTRKLTISKVIEKPKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELH 240

Query: 241 TGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDK 300
           TGIFSAAEQKRIVDHVY LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDK
Sbjct: 241 TGIFSAAEQKRIVDHVYLLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDK 300

Query: 301 NGNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNH 360
           NGNPPGILRSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIYDEGDCIPPHID+H
Sbjct: 301 NGNPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYDEGDCIPPHIDSH 360

Query: 361 DFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVP 420
           DFVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVP
Sbjct: 361 DFVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVP 420

Query: 421 AVPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPF 480
           AVPTKRISITFRR+DESK PIEYA EPDLQGIQPLPYEASEN+VPTSPVSSEREIRRQ F
Sbjct: 421 AVPTKRISITFRRMDESKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQSF 480

Query: 481 RRGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RRGSHMRTRGSGNR+DTRFD RN GR RH+SADRRSRE
Sbjct: 481 RRGSHMRTRGSGNRTDTRFDSRNLGRGRHSSADRRSRE 505

[Close]
BLAST of HG10016377 vs. ExPASy TrEMBL
Match: A0A6J1GFD0 (uncharacterized protein LOC111453671 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453671 PE=4 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.9e-260
Identity = 460/517 (88.97%), Postives = 483/517 (93.42%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDP  
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  +DEN SS  PVE MHESNGNQ+DACDANSLGSWKDEAETNSL SWK
Sbjct: 61  -----------AARTDENSSSTPPVEVMHESNGNQNDACDANSLGSWKDEAETNSLESWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDEYELEKRLV 180
           DGI EGNEADGVPETSSS  SSK+ STKTSGPRMSWADMTQEDEL EEEEDEYE EKR+V
Sbjct: 121 DGINEGNEADGVPETSSSVQSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVV 180

Query: 181 SINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHT 240
           S+NESTRKLTISKVIEK +LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELHT
Sbjct: 181 SVNESTRKLTISKVIEKAKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHT 240

Query: 241 GIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKN 300
           GIFSAAEQKRIVDHVY+LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDKN
Sbjct: 241 GIFSAAEQKRIVDHVYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKN 300

Query: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHD 360
           GNPPGI+RSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIY+EGDCIPPHID+HD
Sbjct: 301 GNPPGIIRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYNEGDCIPPHIDSHD 360

Query: 361 FVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420
           FVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA
Sbjct: 361 FVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420

Query: 421 VPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFR 480
           VPTKRISITFRR+D SK PIEYA EPDLQGIQPLPYEASEN+VPTSPVSSEREIRRQPFR
Sbjct: 421 VPTKRISITFRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFR 480

Query: 481 RGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RGSHMRTRGSGNR+DTRFD RN GR RH+SADRRSRE
Sbjct: 481 RGSHMRTRGSGNRTDTRFDSRNSGRGRHSSADRRSRE 504

[Close]
BLAST of HG10016377 vs. ExPASy TrEMBL
Match: A0A6J1GGD7 (uncharacterized protein LOC111453671 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453671 PE=4 SV=1)

HSP 1 Score: 907.9 bits (2345), Expect = 1.9e-260
Identity = 460/517 (88.97%), Postives = 483/517 (93.42%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLC +CTKLLS+RIRALDP  
Sbjct: 40  MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCRNCTKLLSDRIRALDP-- 99

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  +DEN SS  PVE MHESNGNQ+DACDANSLGSWKDEAETNSL SWK
Sbjct: 100 -----------AARTDENSSSTPPVEVMHESNGNQNDACDANSLGSWKDEAETNSLESWK 159

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDEL-EEEEDEYELEKRLV 180
           DGI EGNEADGVPETSSS  SSK+ STKTSGPRMSWADMTQEDEL EEEEDEYE EKR+V
Sbjct: 160 DGINEGNEADGVPETSSSVQSSKVVSTKTSGPRMSWADMTQEDELEEEEEDEYESEKRVV 219

Query: 181 SINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHT 240
           S+NESTRKLTISKVIEK +LSREQRE+IRF+NVGRKKDFICLERFKGK+VNILEGLELHT
Sbjct: 220 SVNESTRKLTISKVIEKAKLSREQREHIRFINVGRKKDFICLERFKGKLVNILEGLELHT 279

Query: 241 GIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKN 300
           GIFSAAEQKRIVDHVY+LQEMGKKGELRERTFSAPKKWMKGKGRVT+QFGCCYNYAPDKN
Sbjct: 280 GIFSAAEQKRIVDHVYSLQEMGKKGELRERTFSAPKKWMKGKGRVTLQFGCCYNYAPDKN 339

Query: 301 GNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHD 360
           GNPPGI+RSEIVDPIPSLFKVIIRRLV+WHVLPPTCVP+SCIVNIY+EGDCIPPHID+HD
Sbjct: 340 GNPPGIIRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPNSCIVNIYNEGDCIPPHIDSHD 399

Query: 361 FVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 420
           FVRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA
Sbjct: 400 FVRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPA 459

Query: 421 VPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFR 480
           VPTKRISITFRR+D SK PIEYA EPDLQGIQPLPYEASEN+VPTSPVSSEREIRRQPFR
Sbjct: 460 VPTKRISITFRRMDGSKIPIEYAPEPDLQGIQPLPYEASENNVPTSPVSSEREIRRQPFR 519

Query: 481 RGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRRSRE 517
           RGSHMRTRGSGNR+DTRFD RN GR RH+SADRRSRE
Sbjct: 520 RGSHMRTRGSGNRTDTRFDSRNSGRGRHSSADRRSRE 543

[Close]
BLAST of HG10016377 vs. ExPASy TrEMBL
Match: A0A5A7SN55 (Putative alpha-ketoglutarate-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001380 PE=4 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.5e-260
Identity = 459/516 (88.95%), Postives = 478/516 (92.64%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MAISHQHLKLEDPFLHNYKPSEL+IASEFLTTWLPFLS+DLC  CTKLLS+RIR LDP  
Sbjct: 1   MAISHQHLKLEDPFLHNYKPSELKIASEFLTTWLPFLSKDLCGGCTKLLSDRIRTLDP-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  SDEN  SA PVEDMHESNGNQDDA DANSLGSWKDEAETNSLGSWK
Sbjct: 61  -----------ADRSDENSGSAPPVEDMHESNGNQDDAFDANSLGSWKDEAETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVS 180
           DG+  GNEADG PE SSS+L SKLNSTKTSGPRMSWADMTQEDELEEEEDEYE EKRLVS
Sbjct: 121 DGMNGGNEADGGPENSSSDLPSKLNSTKTSGPRMSWADMTQEDELEEEEDEYESEKRLVS 180

Query: 181 INESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTG 240
           +NESTRKLTISKVIE+P+LSREQRE+IRFMNVGRKKDFICLERFKGK+VNILEGLELHT 
Sbjct: 181 LNESTRKLTISKVIERPKLSREQREHIRFMNVGRKKDFICLERFKGKLVNILEGLELHTC 240

Query: 241 IFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG 300
           IFSAAEQ RIVDHVYALQEMGK+GELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG
Sbjct: 241 IFSAAEQTRIVDHVYALQEMGKRGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG 300

Query: 301 NPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF 360
           NPPGILRSEIVDPIPSLFKVIIRRLV+WHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF
Sbjct: 301 NPPGILRSEIVDPIPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF 360

Query: 361 VRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV 420
           VRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV
Sbjct: 361 VRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV 420

Query: 421 PTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRR 480
           PTKRISITFRRIDESKRPIEY+SEPDLQGIQPLPYEA  NDVP SPVSSEREIRRQPFR 
Sbjct: 421 PTKRISITFRRIDESKRPIEYSSEPDLQGIQPLPYEARVNDVPPSPVSSEREIRRQPFRG 480

Query: 481 GSHMRTRGSGNRSDTRFDPRNPGR-ARHNSADRRSR 516
           G HMRTRGSGNR D R+DP+NPGR ARH+SAD++SR
Sbjct: 481 GGHMRTRGSGNRGDPRYDPQNPGRGARHSSADKKSR 503

[Close]
BLAST of HG10016377 vs. ExPASy TrEMBL
Match: A0A0A0KKN4 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G505850 PE=4 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.2e-257
Identity = 457/517 (88.39%), Postives = 474/517 (91.68%), Query Frame = 0 

Query: 1   MAISHQHLKLEDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGL 60
           MA SHQHLKL+DPFLHNYKPSEL+IASEFLTTWLPFLS+DLC DCTKLLS+RIR LD   
Sbjct: 1   MAFSHQHLKLDDPFLHNYKPSELKIASEFLTTWLPFLSKDLCGDCTKLLSDRIRTLDR-- 60

Query: 61  SDEIVELDNVVAVCSDENLSSASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWK 120
                      A  SDEN  S   V+DMHESNGNQDDA DANSLGSWKDEAETNSLGSWK
Sbjct: 61  -----------AGRSDENSGSPPAVDDMHESNGNQDDAFDANSLGSWKDEAETNSLGSWK 120

Query: 121 DGITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVS 180
           DG+  GNEADG PETSSSEL SKLNSTKTSGPRMSWADMTQEDELEEEEDEYE EKRLVS
Sbjct: 121 DGMNAGNEADGGPETSSSELPSKLNSTKTSGPRMSWADMTQEDELEEEEDEYESEKRLVS 180

Query: 181 INESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTG 240
           +NESTRKLTISKVIE+P+LSREQRE+IRFMNVGRKKDFICLERFKGK+VNILEGLELHT 
Sbjct: 181 VNESTRKLTISKVIERPKLSREQREHIRFMNVGRKKDFICLERFKGKLVNILEGLELHTC 240

Query: 241 IFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG 300
           IFSAAEQ RIVDHVYALQEMGK+GELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG
Sbjct: 241 IFSAAEQTRIVDHVYALQEMGKRGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNG 300

Query: 301 NPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF 360
           NPPGILRSEIVDP+PSLFKVIIRRLV+WHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF
Sbjct: 301 NPPGILRSEIVDPLPSLFKVIIRRLVRWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF 360

Query: 361 VRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV 420
           VRPFCTVSFLSECNIVFG+NLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV
Sbjct: 361 VRPFCTVSFLSECNIVFGTNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAV 420

Query: 421 PTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRR 480
           PTKRISITFRRIDESKRPIEYA EPDLQGIQPLPY     DVPTSPVSSEREIRRQPFRR
Sbjct: 421 PTKRISITFRRIDESKRPIEYAPEPDLQGIQPLPY-----DVPTSPVSSEREIRRQPFRR 480

Query: 481 GSHMRTRGSGNRSDTRFDPRNPGR-ARHNSADRRSRE 517
           G HMRTRGSGNR DTR+D RNPGR A HNSADR+SRE
Sbjct: 481 GGHMRTRGSGNRGDTRYDSRNPGRGAHHNSADRKSRE 499

[Close]
BLAST of HG10016377 vs. TAIR 10
Match: AT2G17970.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 567.0 bits (1460), Expect = 1.5e-161
Identity = 307/510 (60.20%), Postives = 371/510 (72.75%), Query Frame = 0 

Query: 12  DPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGLSDEIVELDNVV 71
           DPFL  Y+PSEL+IASEFLT WLPFLS+DLC+DC  LLSNRIR+LDP         +N  
Sbjct: 4   DPFLRQYQPSELKIASEFLTNWLPFLSKDLCKDCNHLLSNRIRSLDPAHCS-----NNTD 63

Query: 72  AVCSDENLSSASPVEDM--HESNGNQDDACDANSLGSWKDEAETNSLGSWKDGITEGNEA 131
            V  +    S S VE+M    ++ N DD  D  S     ++ + +SLGSWK     G+E 
Sbjct: 64  KVDGECKTGSCSVVENMGSERASNNVDDNYDEKSENG--EDCDNHSLGSWK-----GSEI 123

Query: 132 --DGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVSINESTRK 191
                PE  SS L S+    +T+ PRM WADM QEDE +EEE+E E E+      +S+RK
Sbjct: 124 VFGSFPEDFSSVLQSRPAVVETASPRMRWADMAQEDEFDEEEEEEEEER------DSSRK 183

Query: 192 ---LTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTGIFSA 251
               +  K  EKP+LSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA
Sbjct: 184 GFDASSMKTPEKPKLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSA 243

Query: 252 AEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNGNPPG 311
            EQKRIVD VY LQE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPG
Sbjct: 244 VEQKRIVDQVYQLQEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPG 303

Query: 312 ILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPF 371
           IL+ E VDP+P LFKVIIR+L+KWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF+RPF
Sbjct: 304 ILQREEVDPLPHLFKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPF 363

Query: 372 CTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKR 431
           CT+SFLSEC+I+FGSNL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKR
Sbjct: 364 CTISFLSECDILFGSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKR 423

Query: 432 ISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGSHM 491
           ISITFR++DESKRP+ +  EPDLQGI+PLP + + +        S     R     G++ 
Sbjct: 424 ISITFRKMDESKRPVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQ 483

Query: 492 R---TRGSGNRSDTRFDPRNPGR-ARHNSA 511
           R    RG GN  D+R    NP R + HN +
Sbjct: 484 RGHGRRGGGNGYDSR-GYYNPERSSEHNDS 487

[Close]
BLAST of HG10016377 vs. TAIR 10
Match: AT4G36090.3 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 514.2 bits (1323), Expect = 1.2e-145
Identity = 292/511 (57.14%), Postives = 354/511 (69.28%), Query Frame = 0 

Query: 11  EDPFLHNYKPSELRIASEFLTTWLPFLSRDLCEDCTKLLSNRIRALDPGLSDEIVELDNV 70
           ED FL  Y+ SEL+IASEFLT WLPFLSRDLC DC  +LS+RIR+LDP            
Sbjct: 7   EDVFLAKYQSSELKIASEFLTNWLPFLSRDLCNDCAHVLSDRIRSLDP------------ 66

Query: 71  VAVCSDENL--SSASPVEDMH-ESNGNQDDACDANSLGSWKDE--AETNSLGSWKDG-IT 130
              CS+  +   S S V++M  E   N +     N + +  D     ++SL S K G   
Sbjct: 67  -EHCSNGEVKAGSGSMVDNMESEEVDNMESEEVDNKMDNIYDGKLCVSHSLDSLKAGAFV 126

Query: 131 EGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEED-EYELEKRLVSINE 190
            G+ +D V   S +  SS+   ++ +  +MSWADM +ED LEEE+  E EL    V ++ 
Sbjct: 127 FGSLSDSV---SHAMPSSQSTVSEAASAQMSWADMGEEDGLEEEDQKENELGSHGVDVSP 186

Query: 191 STRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTGIFS 250
           S       K  EK +LSRE+RE  RFMNV + K F C E+ +G+ VNILEGLELHTG+FS
Sbjct: 187 SVG--DSMKTPEKRKLSREERERYRFMNVKKMKVFSCYEKVRGRSVNILEGLELHTGVFS 246

Query: 251 AAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNGNPP 310
           A EQK+IVD VY LQE G++GELRERTF+AP KWM+GKGRVT+QFGCCYNYAPDK GNPP
Sbjct: 247 AVEQKKIVDFVYELQEKGRRGELRERTFTAPHKWMRGKGRVTIQFGCCYNYAPDKAGNPP 306

Query: 311 GILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRP 370
           GIL+   VDP+PS+FKVII+RLV WHVLPPTCVPDSCIVNIY+E DCIPPHIDNHDF+RP
Sbjct: 307 GILQRGDVDPMPSIFKVIIKRLVGWHVLPPTCVPDSCIVNIYEEDDCIPPHIDNHDFLRP 366

Query: 371 FCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTK 430
           FCTVSFLSECNI+FGSNL ++GPGEFSG  +IPLPVGSVLVL GNGADVAKHCVPAVPTK
Sbjct: 367 FCTVSFLSECNILFGSNLKVLGPGEFSGSYSIPLPVGSVLVLKGNGADVAKHCVPAVPTK 426

Query: 431 RISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPV-SSEREIRRQPFRRGS 490
           RISITFR++DESKRP+ +  EPDL+ I+PLPYE +    P   V SS R    Q     +
Sbjct: 427 RISITFRKMDESKRPVGFTPEPDLEEIKPLPYEHTTPSTPADAVISSSRSSIDQNGSNHN 486

Query: 491 HMRTRGSGNRSDTRFDPRNPGRARHNSADRR 514
           +    G G++     D  +  R R +S  RR
Sbjct: 487 NRTAHGGGSKYRRSRDYHSESRERSSSGQRR 499

[Close]
BLAST of HG10016377 vs. TAIR 10
Match: AT2G17970.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-139
Identity = 262/437 (59.95%), Postives = 320/437 (73.23%), Query Frame = 0 

Query: 81  SASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGITEGNEADGVPETSSSEL 140
           +++ V+D ++      + CD +SLGSWK        GS+             PE  SS L
Sbjct: 6   ASNNVDDNYDEKSENGEDCDNHSLGSWKGSEIV--FGSF-------------PEDFSSVL 65

Query: 141 SSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVSINESTRK---LTISKVIEKP 200
            S+    +T+ PRM WADM QEDE +EEE+E E E+      +S+RK    +  K  EKP
Sbjct: 66  QSRPAVVETASPRMRWADMAQEDEFDEEEEEEEEER------DSSRKGFDASSMKTPEKP 125

Query: 201 QLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTGIFSAAEQKRIVDHVYAL 260
           +LSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY L
Sbjct: 126 KLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVYQL 185

Query: 261 QEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNGNPPGILRSEIVDPIPSL 320
           QE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPGIL+ E VDP+P L
Sbjct: 186 QEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLPHL 245

Query: 321 FKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLSECNIVF 380
           FKVIIR+L+KWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF+RPFCT+SFLSEC+I+F
Sbjct: 246 FKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILF 305

Query: 381 GSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDESKR 440
           GSNL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR++DESKR
Sbjct: 306 GSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKR 365

Query: 441 PIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGSHMR---TRGSGNRSD 500
           P+ +  EPDLQGI+PLP + + +        S     R     G++ R    RG GN  D
Sbjct: 366 PVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNGYD 413

Query: 501 TRFDPRNPGR-ARHNSA 511
           +R    NP R + HN +
Sbjct: 426 SR-GYYNPERSSEHNDS 413

[Close]
BLAST of HG10016377 vs. TAIR 10
Match: AT2G17970.3 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.3e-139
Identity = 262/437 (59.95%), Postives = 320/437 (73.23%), Query Frame = 0 

Query: 81  SASPVEDMHESNGNQDDACDANSLGSWKDEAETNSLGSWKDGITEGNEADGVPETSSSEL 140
           +++ V+D ++      + CD +SLGSWK        GS+             PE  SS L
Sbjct: 6   ASNNVDDNYDEKSENGEDCDNHSLGSWKGSEIV--FGSF-------------PEDFSSVL 65

Query: 141 SSKLNSTKTSGPRMSWADMTQEDELEEEEDEYELEKRLVSINESTRK---LTISKVIEKP 200
            S+    +T+ PRM WADM QEDE +EEE+E E E+      +S+RK    +  K  EKP
Sbjct: 66  QSRPAVVETASPRMRWADMAQEDEFDEEEEEEEEER------DSSRKGFDASSMKTPEKP 125

Query: 201 QLSREQREYIRFMNVGRKKDFICLERFKGKVVNILEGLELHTGIFSAAEQKRIVDHVYAL 260
           +LSR+QRE +R +NV RKKDFICLER KGK+VN+L+GLELHTG+FSA EQKRIVD VY L
Sbjct: 126 KLSRDQRENLRLINVKRKKDFICLERVKGKIVNVLDGLELHTGVFSAVEQKRIVDQVYQL 185

Query: 261 QEMGKKGELRERTFSAPKKWMKGKGRVTMQFGCCYNYAPDKNGNPPGILRSEIVDPIPSL 320
           QE G++GEL++RTF+AP KWM+GKGR T+QFGCCYNYAPD+ GNPPGIL+ E VDP+P L
Sbjct: 186 QEKGRRGELKKRTFTAPHKWMRGKGRETIQFGCCYNYAPDRAGNPPGILQREEVDPLPHL 245

Query: 321 FKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFVRPFCTVSFLSECNIVF 380
           FKVIIR+L+KWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDF+RPFCT+SFLSEC+I+F
Sbjct: 246 FKVIIRKLIKWHVLPPTCVPDSCIVNIYDEGDCIPPHIDNHDFLRPFCTISFLSECDILF 305

Query: 381 GSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRRIDESKR 440
           GSNL + GPG+FSG  +IPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFR++DESKR
Sbjct: 306 GSNLKVEGPGDFSGSYSIPLPVGSVLVLNGNGADVAKHCVPAVPTKRISITFRKMDESKR 365

Query: 441 PIEYASEPDLQGIQPLPYEASENDVPTSPVSSEREIRRQPFRRGSHMR---TRGSGNRSD 500
           P+ +  EPDLQGI+PLP + + +        S     R     G++ R    RG GN  D
Sbjct: 366 PVWFTPEPDLQGIEPLPLDLNRS-------GSTSRFSRLNNHNGTNQRGHGRRGGGNGYD 413

Query: 501 TRFDPRNPGR-ARHNSA 511
           +R    NP R + HN +
Sbjct: 426 SR-GYYNPERSSEHNDS 413

[Close]
BLAST of HG10016377 vs. TAIR 10
Match: AT4G36090.2 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 458.4 bits (1178), Expect = 7.8e-129
Identity = 247/404 (61.14%), Postives = 297/404 (73.51%), Query Frame = 0 

Query: 113 TNSLGSWKDG-ITEGNEADGVPETSSSELSSKLNSTKTSGPRMSWADMTQEDELEEEED- 172
           ++SL S K G    G+ +D V   S +  SS+   ++ +  +MSWADM +ED LEEE+  
Sbjct: 33  SHSLDSLKAGAFVFGSLSDSV---SHAMPSSQSTVSEAASAQMSWADMGEEDGLEEEDQK 92

Query: 173 EYELEKRLVSINESTRKLTISKVIEKPQLSREQREYIRFMNVGRKKDFICLERFKGKVVN 232
           E EL    V ++ S       K  EK +LSRE+RE  RFMNV + K F C E+ +G+ VN
Sbjct: 93  ENELGSHGVDVSPSVG--DSMKTPEKRKLSREERERYRFMNVKKMKVFSCYEKVRGRSVN 152

Query: 233 ILEGLELHTGIFSAAEQKRIVDHVYALQEMGKKGELRERTFSAPKKWMKGKGRVTMQFGC 292
           ILEGLELHTG+FSA EQK+IVD VY LQE G++GELRERTF+AP KWM+GKGRVT+QFGC
Sbjct: 153 ILEGLELHTGVFSAVEQKKIVDFVYELQEKGRRGELRERTFTAPHKWMRGKGRVTIQFGC 212

Query: 293 CYNYAPDKNGNPPGILRSEIVDPIPSLFKVIIRRLVKWHVLPPTCVPDSCIVNIYDEGDC 352
           CYNYAPDK GNPPGIL+   VDP+PS+FKVII+RLV WHVLPPTCVPDSCIVNIY+E DC
Sbjct: 213 CYNYAPDKAGNPPGILQRGDVDPMPSIFKVIIKRLVGWHVLPPTCVPDSCIVNIYEEDDC 272

Query: 353 IPPHIDNHDFVRPFCTVSFLSECNIVFGSNLSIVGPGEFSGPIAIPLPVGSVLVLNGNGA 412
           IPPHIDNHDF+RPFCTVSFLSECNI+FGSNL ++GPGEFSG  +IPLPVGSVLVL GNGA
Sbjct: 273 IPPHIDNHDFLRPFCTVSFLSECNILFGSNLKVLGPGEFSGSYSIPLPVGSVLVLKGNGA 332

Query: 413 DVAKHCVPAVPTKRISITFRRIDESKRPIEYASEPDLQGIQPLPYEASENDVPTSPV-SS 472
           DVAKHCVPAVPTKRISITFR++DESKRP+ +  EPDL+ I+PLPYE +    P   V SS
Sbjct: 333 DVAKHCVPAVPTKRISITFRKMDESKRPVGFTPEPDLEEIKPLPYEHTTPSTPADAVISS 392

Query: 473 EREIRRQPFRRGSHMRTRGSGNRSDTRFDPRNPGRARHNSADRR 514
            R    Q     ++    G G++     D  +  R R +S  RR
Sbjct: 393 SRSSIDQNGSNHNNRTAHGGGSKYRRSRDYHSESRERSSSGQRR 431

The following BLAST results are available for this feature:
BLAST of HG10016377 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038882153.16.3e-27493.02RNA demethylase ALKBH9B [Benincasa hispida][more]
KAG7034097.12.9e-27191.68RNA demethylase ALKBH5 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023544551.12.2e-26389.75uncharacterized protein LOC111804096 [Cucurbita pepo subsp. pepo] >XP_023544552.... [more]
XP_022979042.11.0e-26089.19RNA demethylase ALKBH5 [Cucurbita maxima] >XP_022979043.1 RNA demethylase ALKBH5... [more]
XP_022950629.14.0e-26088.97uncharacterized protein LOC111453671 isoform X1 [Cucurbita moschata][more]
BLAST of HG10016377 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SL492.2e-16060.20RNA demethylase ALKBH9B OS=Arabidopsis thaliana OX=3702 GN=ALKBH9B PE=1 SV=1[more]
Q9ZT923.4e-2833.79RNA demethylase ALKBH10B OS=Arabidopsis thaliana OX=3702 GN=ALKBH10B PE=1 SV=1[more]
Q66JG82.0e-1232.21RNA demethylase ALKBH5 OS=Xenopus tropicalis OX=8364 GN=alkbh5 PE=2 SV=1[more]
BLAST of HG10016377 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1IMQ35.1e-26189.19RNA demethylase ALKBH5 OS=Cucurbita maxima OX=3661 GN=LOC111478802 PE=4 SV=1[more]
A0A6J1GFD01.9e-26088.97uncharacterized protein LOC111453671 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1GGD71.9e-26088.97uncharacterized protein LOC111453671 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A5A7SN552.5e-26088.95Putative alpha-ketoglutarate-dependent dioxygenase OS=Cucumis melo var. makuwa O... [more]
A0A0A0KKN41.2e-25788.39Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G... [more]
BLAST of HG10016377 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G17970.11.5e-16160.202-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G36090.31.2e-14557.14oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
AT2G17970.21.3e-13959.952-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT2G17970.31.3e-13959.952-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G36090.27.8e-12961.14oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 162..182
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 81..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 440..516
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 487..516
NoneNo IPR availablePANTHERPTHR31447:SF5RNA DEMETHYLASE ALKBH9Bcoord: 5..513
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 231..432
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 219..440
e-value: 3.5E-74
score: 251.1
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 235..430
e-value: 2.4E-14
score: 54.0
IPR044842RNA demethylase ALKBH9B/ALKBH10B-likePANTHERPTHR31447HYDROXYPROLINE-RICH GLYCOPROTEIN FAMILY PROTEIN-RELATEDcoord: 5..513
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 336..433
score: 10.080736

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016377.1HG10016377.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070988 demethylation
biological_process GO:0032259 methylation
biological_process GO:0006402 mRNA catabolic process
molecular_function GO:0032451 demethylase activity
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0003729 mRNA binding