HG10016352 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016352
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
Descriptionnitrate regulatory gene2 protein isoform X2
LocationChr03: 4385496 .. 4388290 (-)
RNA-Seq ExpressionHG10016352
SyntenyHG10016352
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTGCTGTTACTCGAGACTAGAAAGAGAAGAAACTGTGTCGAGGTGCAAAGCAAGAAAGAGGTACATGAAACAGTTGGTGAAGGCGAGGCAAGCTTTTTCTGCTTCTCACAGCTTGTACATTCGTTCTCTTCGTGGCACCGGCGCCGCTCTCCGGCAGTTTTCCAATGCAGAGACCTACATTCGCCGCCCTCAGCAGCAGCAGCAGCAGCAGAACTACCACCACCACCTCCCGCCGGTGCTTCCATCTCCAACCCCACGAACGCCGCCGCCTCCTCCGCCTCCTCATATCCCCATGAGTCCTAGTTCCGACACCTGGACATCGATCACGGCCTCCCCGGCACTACCTCCGCCGCCGCCGCCGCCACCGTCATCCACTTGGGATTTCTGGGACCCTTTTGTGCCGTCGACATCTCGCACTGTGACTGAGGAAGAATGGGAGGCTACCACCATTGCATCGGAGGCTATAGTCACTGTAACAGGGGCGGCTAGCTCAGCACCGCCGCCGTCAGTGGTGAGTGGTTTTTCAAAGGACACTTCAAGCACTGAGCTTGCTATGGTAGTGTCGAGGAACACCAAAGATCTGGTGGAGATTATTAAGGAGCTTGATGAGTACTTTCTCAAAGCTGCTGATGCTGGTGCTCAGCTCTCCTTGCTTTTGGAAGTCCCCACTTTTTCTCCTCAGAATAAAGGAGGTAAATCTAATATCAAAGAAGTAGAAAATCAATCAAATAACAGTTTTCTCTTCCAATTTCTCTAATATCATTCAACTCACATTCCTCAAATCACAAAGTACCCTAATGAGTTCCATATCATGTCGCTAGCAGGGCAGGCTTACAGTAATGGGTGGAGCTTGAGCCCTTCATTGAGGGTGTGGGGTTCAAACACAAAACCAAATGATGCATTTGGTAAGTTAAACGGAGAAATAACAGCAACAAATATGGGAAATGGGTGTGGTGGAAATAGCAGCAGCCACTGTTCAACAGTGGAGAAGCTGTACGCTTGGGAGAAAAAACTGTACCAAGAGGTCAAGGTAATGCTTTCACTTTCGCATATCTCAATCAAATCTCTAGTCACATTGGTGGAATAATATGACATTTTCGTACTTTAAATAAAGTCTTCAATTTGAAATGATTCTTTTCACCTTTTTTTTTTCCTTTTCTTCTACTTAATGGGGTCCAACATAACCAAACAAAGATGAAACTTTAGCATTTCTCAATGCATTTGAAGGCATTGAAGAACGGAGTTGTGGAATTCTATGTACTAAAGTTTGTGAAATTATGTTGAGTGGCTTTTTTAGACTGCCGAGGCCACAAGGATAGAGCATGAGAAGAAGGTGGAACAGTTGAGGAAGCTAGAGCTGAAGAGGGCTGACTATGTAAAGACTGAGAAGACTAAGAAAGAAGTTGAAAAGCTGGAGTCACAGATGATGGTTGCTTCTCAGGCCATTGAAACCACATCTGCTGAAATCATCAAGCTAAGAGAAATTGAACTCTATCCACAACTCCTTGAGCTTGTCAAAGGGTTAGTCTCTTTCATACATCTATTTTTGCCTCAACAGAAATAAGAAATCACTAATCATTTGACTGTATTTTGATGATTGCTTGATATTTAAGCTGTAAAGTCCATAGCTTCTGTGTTTACCAGTATTTTATACTACCCTGTGTACAGGGAAGAACTCAGAACAATTCAAATTGGCTGAGGATTTTAAAAAGCCATTATTTTCTATTTCACTATCTAATTGGGATATGATTGATTTCAATTGGTTGACCTAGACAACCAGGGAGAGTCCATCATTTTTGGCATGATTTCATTACCTTCAACTATCGGTCTTTTATTCTGTGGATATTTTCACTGTTCACTTGTACAGTTTTGCTACCTTCTGGTATCTCAGGAAGCCTAAATGGAAAATACTAAGCATATTTTGGATATCTTCATGGAAATAATAGTAATGCTGTCTTCACTTGGAAGTATTCTATTCTAGACACTAAGATTTTCCCTGGTTTCTACAGATTGATGTGCATGTGGAGAAGTATGTACGAGTCTCATCAGGTCCAAACACACATTGTTGAACAGCTCAAATATCTAAATATCATACCATCTGCCGAGCCCACATCCGAGATCCACCGCCAATCAACTCTCCAGCTTGAGCTTGAAGTCCAGCAATGGCATGCCTCTTTTTGCAATCTAGTGAAAGCTCAACGGGATTATGTCCAGTCTCTTACAGGCTGGCTACGGCTTTGCCTTTTCCAGATAAGTGGAAACCCATTGTTAAGAACAGGTCAAGATTCTAGAATTTACTCACTGTGTGAAGAATGGAACCTCGCAGTTGATAGGATTCCAGACAAAGTGGCTTCTGAAGGAATCAAGAGCTTCTTAACCGTAGTTCATGCCATTGTTGTTCAACAAGCTGAGGAATATAGGCAAAAGAAGAAGGCAGATTCTGCCTCAAAAGAGCTTGACAAGAGAGCTTCTGAGCTAAGATCACTAGAAAGCAAATATGTCTCACATTCAATGCGAGAATGCTCGGCATCAACAAGAGGGAGAGACCCAGTTCAGGAGAAGCAAAACAAGGTGGAGAATTTAAGAACAAAAGCTCGAGACGAGAAACAAAAGCATGAAAACTTAGTCAGTGTGACAAGGTCAATGACGATAAATAACTTGCAGATGAGTTTTCCCCATGTTTTCCAGGCAATGGTTGGGTTTTCCAGTGTATGTATGCACGCATACGAGGCAGTTTATAATCAAACTAAAAATACTGACCAGCATGAGGTGAAGAGATTACTACCTTAA

mRNA sequence

ATGGGTTGCTGTTACTCGAGACTAGAAAGAGAAGAAACTGTGTCGAGGTGCAAAGCAAGAAAGAGGTACATGAAACAGTTGGTGAAGGCGAGGCAAGCTTTTTCTGCTTCTCACAGCTTGTACATTCGTTCTCTTCGTGGCACCGGCGCCGCTCTCCGGCAGTTTTCCAATGCAGAGACCTACATTCGCCGCCCTCAGCAGCAGCAGCAGCAGCAGAACTACCACCACCACCTCCCGCCGGTGCTTCCATCTCCAACCCCACGAACGCCGCCGCCTCCTCCGCCTCCTCATATCCCCATGAGTCCTAGTTCCGACACCTGGACATCGATCACGGCCTCCCCGGCACTACCTCCGCCGCCGCCGCCGCCACCGTCATCCACTTGGGATTTCTGGGACCCTTTTGTGCCGTCGACATCTCGCACTGTGACTGAGGAAGAATGGGAGGCTACCACCATTGCATCGGAGGCTATAGTCACTGTAACAGGGGCGGCTAGCTCAGCACCGCCGCCGTCAGTGGTGAGTGGTTTTTCAAAGGACACTTCAAGCACTGAGCTTGCTATGGTAGTGTCGAGGAACACCAAAGATCTGGTGGAGATTATTAAGGAGCTTGATGAGTACTTTCTCAAAGCTGCTGATGCTGGTGCTCAGCTCTCCTTGCTTTTGGAAGTCCCCACTTTTTCTCCTCAGAATAAAGGAGGGCAGGCTTACAGTAATGGGTGGAGCTTGAGCCCTTCATTGAGGGTGTGGGGTTCAAACACAAAACCAAATGATGCATTTGGTAAGTTAAACGGAGAAATAACAGCAACAAATATGGGAAATGGGTGTGGTGGAAATAGCAGCAGCCACTGTTCAACAGTGGAGAAGCTGTACGCTTGGGAGAAAAAACTGTACCAAGAGGTCAAGACTGCCGAGGCCACAAGGATAGAGCATGAGAAGAAGGTGGAACAGTTGAGGAAGCTAGAGCTGAAGAGGGCTGACTATGTAAAGACTGAGAAGACTAAGAAAGAAGTTGAAAAGCTGGAGTCACAGATGATGGTTGCTTCTCAGGCCATTGAAACCACATCTGCTGAAATCATCAAGCTAAGAGAAATTGAACTCTATCCACAACTCCTTGAGCTTGTCAAAGGATTGATGTGCATGTGGAGAAGTATGTACGAGTCTCATCAGGTCCAAACACACATTGTTGAACAGCTCAAATATCTAAATATCATACCATCTGCCGAGCCCACATCCGAGATCCACCGCCAATCAACTCTCCAGCTTGAGCTTGAAGTCCAGCAATGGCATGCCTCTTTTTGCAATCTAGTGAAAGCTCAACGGGATTATGTCCAGTCTCTTACAGGCTGGCTACGGCTTTGCCTTTTCCAGATAAGTGGAAACCCATTGTTAAGAACAGGTCAAGATTCTAGAATTTACTCACTGTGTGAAGAATGGAACCTCGCAGTTGATAGGATTCCAGACAAAGTGGCTTCTGAAGGAATCAAGAGCTTCTTAACCGTAGTTCATGCCATTGTTGTTCAACAAGCTGAGGAATATAGGCAAAAGAAGAAGGCAGATTCTGCCTCAAAAGAGCTTGACAAGAGAGCTTCTGAGCTAAGATCACTAGAAAGCAAATATGTCTCACATTCAATGCGAGAATGCTCGGCATCAACAAGAGGGAGAGACCCAGTTCAGGAGAAGCAAAACAAGGTGGAGAATTTAAGAACAAAAGCTCGAGACGAGAAACAAAAGCATGAAAACTTAGTCAGTGTGACAAGGTCAATGACGATAAATAACTTGCAGATGAGTTTTCCCCATGTTTTCCAGGCAATGGTTGGGTTTTCCAGTGTATGTATGCACGCATACGAGGCAGTTTATAATCAAACTAAAAATACTGACCAGCATGAGGTGAAGAGATTACTACCTTAA

Coding sequence (CDS)

ATGGGTTGCTGTTACTCGAGACTAGAAAGAGAAGAAACTGTGTCGAGGTGCAAAGCAAGAAAGAGGTACATGAAACAGTTGGTGAAGGCGAGGCAAGCTTTTTCTGCTTCTCACAGCTTGTACATTCGTTCTCTTCGTGGCACCGGCGCCGCTCTCCGGCAGTTTTCCAATGCAGAGACCTACATTCGCCGCCCTCAGCAGCAGCAGCAGCAGCAGAACTACCACCACCACCTCCCGCCGGTGCTTCCATCTCCAACCCCACGAACGCCGCCGCCTCCTCCGCCTCCTCATATCCCCATGAGTCCTAGTTCCGACACCTGGACATCGATCACGGCCTCCCCGGCACTACCTCCGCCGCCGCCGCCGCCACCGTCATCCACTTGGGATTTCTGGGACCCTTTTGTGCCGTCGACATCTCGCACTGTGACTGAGGAAGAATGGGAGGCTACCACCATTGCATCGGAGGCTATAGTCACTGTAACAGGGGCGGCTAGCTCAGCACCGCCGCCGTCAGTGGTGAGTGGTTTTTCAAAGGACACTTCAAGCACTGAGCTTGCTATGGTAGTGTCGAGGAACACCAAAGATCTGGTGGAGATTATTAAGGAGCTTGATGAGTACTTTCTCAAAGCTGCTGATGCTGGTGCTCAGCTCTCCTTGCTTTTGGAAGTCCCCACTTTTTCTCCTCAGAATAAAGGAGGGCAGGCTTACAGTAATGGGTGGAGCTTGAGCCCTTCATTGAGGGTGTGGGGTTCAAACACAAAACCAAATGATGCATTTGGTAAGTTAAACGGAGAAATAACAGCAACAAATATGGGAAATGGGTGTGGTGGAAATAGCAGCAGCCACTGTTCAACAGTGGAGAAGCTGTACGCTTGGGAGAAAAAACTGTACCAAGAGGTCAAGACTGCCGAGGCCACAAGGATAGAGCATGAGAAGAAGGTGGAACAGTTGAGGAAGCTAGAGCTGAAGAGGGCTGACTATGTAAAGACTGAGAAGACTAAGAAAGAAGTTGAAAAGCTGGAGTCACAGATGATGGTTGCTTCTCAGGCCATTGAAACCACATCTGCTGAAATCATCAAGCTAAGAGAAATTGAACTCTATCCACAACTCCTTGAGCTTGTCAAAGGATTGATGTGCATGTGGAGAAGTATGTACGAGTCTCATCAGGTCCAAACACACATTGTTGAACAGCTCAAATATCTAAATATCATACCATCTGCCGAGCCCACATCCGAGATCCACCGCCAATCAACTCTCCAGCTTGAGCTTGAAGTCCAGCAATGGCATGCCTCTTTTTGCAATCTAGTGAAAGCTCAACGGGATTATGTCCAGTCTCTTACAGGCTGGCTACGGCTTTGCCTTTTCCAGATAAGTGGAAACCCATTGTTAAGAACAGGTCAAGATTCTAGAATTTACTCACTGTGTGAAGAATGGAACCTCGCAGTTGATAGGATTCCAGACAAAGTGGCTTCTGAAGGAATCAAGAGCTTCTTAACCGTAGTTCATGCCATTGTTGTTCAACAAGCTGAGGAATATAGGCAAAAGAAGAAGGCAGATTCTGCCTCAAAAGAGCTTGACAAGAGAGCTTCTGAGCTAAGATCACTAGAAAGCAAATATGTCTCACATTCAATGCGAGAATGCTCGGCATCAACAAGAGGGAGAGACCCAGTTCAGGAGAAGCAAAACAAGGTGGAGAATTTAAGAACAAAAGCTCGAGACGAGAAACAAAAGCATGAAAACTTAGTCAGTGTGACAAGGTCAATGACGATAAATAACTTGCAGATGAGTTTTCCCCATGTTTTCCAGGCAATGGTTGGGTTTTCCAGTGTATGTATGCACGCATACGAGGCAGTTTATAATCAAACTAAAAATACTGACCAGCATGAGGTGAAGAGATTACTACCTTAA

Protein sequence

MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAETYIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPPPPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDTSSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGWSLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYVSHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP
Homology
BLAST of HG10016352 vs. NCBI nr
Match: XP_038880957.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 1179.1 bits (3049), Expect = 0.0e+00
Identity = 619/635 (97.48%), Postives = 624/635 (98.27%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRP  QQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRP--QQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS QNKGGQ Y+NGW
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQNKGGQGYNNGW 240

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SLSPSL VWGSNTK NDAFGKLNGEIT TNMGNGC GNSSSHCSTVEKLYAWEKKLYQEV
Sbjct: 241 SLSPSLWVWGSNTKSNDAFGKLNGEITPTNMGNGCAGNSSSHCSTVEKLYAWEKKLYQEV 300

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI+K
Sbjct: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIVK 360

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LREIELYPQLLE VKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ
Sbjct: 361 LREIELYPQLLEFVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL
Sbjct: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKEL+KRA ELRSLESKYV
Sbjct: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELEKRAIELRSLESKYV 540

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKV+NLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 541 SHSMRECSASTRGRDPVQEKQNKVDNLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 FQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 633

BLAST of HG10016352 vs. NCBI nr
Match: XP_038880956.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 619/636 (97.33%), Postives = 624/636 (98.11%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRP  QQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRP--QQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKG-GQAYSNG 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS QNKG GQ Y+NG
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQNKGAGQGYNNG 240

Query: 241 WSLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQE 300
           WSLSPSL VWGSNTK NDAFGKLNGEIT TNMGNGC GNSSSHCSTVEKLYAWEKKLYQE
Sbjct: 241 WSLSPSLWVWGSNTKSNDAFGKLNGEITPTNMGNGCAGNSSSHCSTVEKLYAWEKKLYQE 300

Query: 301 VKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEII 360
           VKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI+
Sbjct: 301 VKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIV 360

Query: 361 KLREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTL 420
           KLREIELYPQLLE VKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTL
Sbjct: 361 KLREIELYPQLLEFVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTL 420

Query: 421 QLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWN 480
           QLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWN
Sbjct: 421 QLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWN 480

Query: 481 LAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKY 540
           LAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKEL+KRA ELRSLESKY
Sbjct: 481 LAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELEKRAIELRSLESKY 540

Query: 541 VSHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPH 600
           VSHSMRECSASTRGRDPVQEKQNKV+NLRTKARDEKQKHENLVSVTRSMTINNLQMSFPH
Sbjct: 541 VSHSMRECSASTRGRDPVQEKQNKVDNLRTKARDEKQKHENLVSVTRSMTINNLQMSFPH 600

Query: 601 VFQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           VFQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 VFQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 634

BLAST of HG10016352 vs. NCBI nr
Match: XP_038880958.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X3 [Benincasa hispida])

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 615/636 (96.70%), Postives = 621/636 (97.64%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRP     QQ+YHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRP-----QQHYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKG-GQAYSNG 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS QNKG GQ Y+NG
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQNKGAGQGYNNG 240

Query: 241 WSLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQE 300
           WSLSPSL VWGSNTK NDAFGKLNGEIT TNMGNGC GNSSSHCSTVEKLYAWEKKLYQE
Sbjct: 241 WSLSPSLWVWGSNTKSNDAFGKLNGEITPTNMGNGCAGNSSSHCSTVEKLYAWEKKLYQE 300

Query: 301 VKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEII 360
           VKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI+
Sbjct: 301 VKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIV 360

Query: 361 KLREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTL 420
           KLREIELYPQLLE VKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTL
Sbjct: 361 KLREIELYPQLLEFVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTL 420

Query: 421 QLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWN 480
           QLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWN
Sbjct: 421 QLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWN 480

Query: 481 LAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKY 540
           LAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKEL+KRA ELRSLESKY
Sbjct: 481 LAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELEKRAIELRSLESKY 540

Query: 541 VSHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPH 600
           VSHSMRECSASTRGRDPVQEKQNKV+NLRTKARDEKQKHENLVSVTRSMTINNLQMSFPH
Sbjct: 541 VSHSMRECSASTRGRDPVQEKQNKVDNLRTKARDEKQKHENLVSVTRSMTINNLQMSFPH 600

Query: 601 VFQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           VFQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 VFQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 631

BLAST of HG10016352 vs. NCBI nr
Match: XP_011658056.1 (protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X2 [Cucumis sativus])

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 610/635 (96.06%), Postives = 616/635 (97.01%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRPQ  Q     HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRPQPHQ-----HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS Q KGGQ Y+NGW
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQKKGGQVYNNGW 240

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SL      WGSNTKPNDAFGKLNGE+TATNMGNG GGNS SHCSTVEKLYAWEKKLYQEV
Sbjct: 241 SL------WGSNTKPNDAFGKLNGELTATNMGNGYGGNSRSHCSTVEKLYAWEKKLYQEV 300

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK
Sbjct: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ
Sbjct: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL
Sbjct: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRA+ELRSLESKYV
Sbjct: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRATELRSLESKYV 540

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKV+NLRTKA+DEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 541 SHSMRECSASTRGRDPVQEKQNKVDNLRTKAQDEKQKHENLVSVTRSMTINNLQMSFPHV 600

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 FQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 624

BLAST of HG10016352 vs. NCBI nr
Match: KAA0025658.1 (Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-containing protein [Cucumis melo var. makuwa] >TYK12531.1 Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 610/635 (96.06%), Postives = 615/635 (96.85%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 69  MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 128

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRPQ  Q     HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 129 YIRRPQPHQ-----HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 188

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 189 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 248

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS Q KGGQ Y+NGW
Sbjct: 249 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQKKGGQVYNNGW 308

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SL      WGSNTKPNDAFGKLNGEI ATNMGNG GGNS SHCSTVEKLYAWEKKLYQEV
Sbjct: 309 SL------WGSNTKPNDAFGKLNGEIIATNMGNGYGGNSGSHCSTVEKLYAWEKKLYQEV 368

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK
Sbjct: 369 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 428

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ
Sbjct: 429 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 488

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL
Sbjct: 489 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 548

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRA+ELRSLESKYV
Sbjct: 549 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRATELRSLESKYV 608

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKV+NLRTKA+DEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 609 SHSMRECSASTRGRDPVQEKQNKVDNLRTKAQDEKQKHENLVSVTRSMTINNLQMSFPHV 668

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 669 FQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 692

BLAST of HG10016352 vs. ExPASy Swiss-Prot
Match: A0A178VBJ0 (Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=APSR1 PE=2 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 2.8e-202
Identity = 413/667 (61.92%), Postives = 497/667 (74.51%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCC SR++ +E VSRCKARKRY+K LVKARQ  S SH+LY+RSLR  G++L  FS+ ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSD--TWTSITAS----- 120
            +           + HH P   PSP+P  PPPP PP  P+SP S+  TWT+ T S     
Sbjct: 61  PL-----------HLHHNP---PSPSPPPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPP 120

Query: 121 PALPPPPPPPPSSTWDFWDPFVPSTSRTVTEEEWE---------ATTIASEAIVTVTGAA 180
           P  PPPPPPPPSSTWDFWDPF+P    + +EEEWE         AT   S+A VT     
Sbjct: 121 PPPPPPPPPPPSSTWDFWDPFIPPPPSS-SEEEWEEETTTATRTATGTGSDAAVTTAPTT 180

Query: 181 SSAPPPSVVSGFSKDTSST-----ELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSL 240
           ++    SVVSGFSKDT +T     ELA+VVSRN KDL+EIIKE+DEYFLKAAD+GA LS 
Sbjct: 181 ATPQASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSS 240

Query: 241 LLEVPT----FSPQNKGGQAYSNG---WSLSPSLRVWGSNTKPNDAFGKLNGEITATNMG 300
           LLE+ T    FS  +K G+ YS+     +L+P+   W     P+    KL+      N G
Sbjct: 241 LLEISTSITDFSGHSKSGKMYSSSNYECNLNPT-SFWTRGFAPS----KLS---EYRNAG 300

Query: 301 NGCGGNS--SSHCSTVEKLYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADYVKT 360
              GGN    SH STV++LYAWEKKLYQEVK AE+ +++HEKKVEQ+R+LE+KRA+YVKT
Sbjct: 301 GVIGGNCIVGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKT 360

Query: 361 EKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYESHQV 420
           EK KK+VEKLESQ+ V+SQAI++ S EIIKLRE ELYPQL+ELVKGLMCMWRSMYESHQV
Sbjct: 361 EKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQV 420

Query: 421 QTHIVEQLKYLNIIPSAEPTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSLTGWL 480
           QTHIV+QLKYLN IPS EPTSE+HRQSTLQLELEVQQWH SFCNLVKAQRDY+QSLTGWL
Sbjct: 421 QTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWL 480

Query: 481 RLCLFQISGNPLLRTGQDSRIYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVVQQAE 540
           RL LFQ S NPL+R+  +S+IYS CEEW+LA+DRIPDKVASEGIKSFLT VH IV QQA+
Sbjct: 481 RLSLFQFSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQAD 540

Query: 541 EYRQKKKADSASKELDKRASELRSLESKYVSHSMRECSASTRGRDPVQEKQNKVENLRTK 600
           E++QKK+ +S  K+ +K+++ LR+LESKY  +S+ E    +R ++PV EK+ KVE L+ K
Sbjct: 541 EHKQKKRTESMLKDFEKKSASLRALESKYSPYSVPE----SRKKNPVIEKRVKVEMLKGK 600

Query: 601 ARDEKQKHENLVSVTRSMTINNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKN--TDQH 636
           A +EK KHE  VSVTR+MT+NNLQM FPHVFQAMVGFSSVCM A+E+VYNQ K+   DQ 
Sbjct: 601 AEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQE 640

BLAST of HG10016352 vs. ExPASy Swiss-Prot
Match: Q9AQW1 (Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL2 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 8.5e-82
Identity = 273/778 (35.09%), Postives = 373/778 (47.94%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGC  S++E+E+TV RCK R+R+MK+ V +RQ  +++H+ Y+RSLR T AAL +F+    
Sbjct: 1   MGCTASKVEQEDTVRRCKERRRHMKEAVASRQQLASAHADYLRSLRLTAAALSRFA---- 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVL---------PSPTPRTP--------------------- 120
                 Q        HH  PVL         P+PTP  P                     
Sbjct: 61  ------QGHPSLAVSHHTAPVLLTTAAPALAPTPTPPPPSSTASSSLPPPTPLLPKHQQA 120

Query: 121 PPPPPP---HIPMSPSS---------------DTWTSITASPA-----LPPPPPPPPSST 180
           PPPPPP   H P  P +                   S  ASPA      P    P  SS 
Sbjct: 121 PPPPPPTQSHQPPPPVAVRAPRGGPRRLKVPHILSDSSVASPARSSFRKPVVGTPSSSSA 180

Query: 181 WDFWDPFVPSTS-------------------RTVTEEE---------------------- 240
           WD W+ F P +                    R + EEE                      
Sbjct: 181 WD-WENFYPPSPPDSEFFDRRKADLEEANRLRELEEEEKARGYLHPHHLKEEDEVDDDDD 240

Query: 241 ----------WE------ATTIASEAIV---------------------TVTGAASSAPP 300
                     WE      A+T  SE                        T     ++AP 
Sbjct: 241 EREEEMHCGGWEDDDDHYASTTTSETRSEEGEMGNRSECGFAARSEYGGTAPSEYAAAPL 300

Query: 301 P----------SVVSGFSKDTSSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSL 360
           P                S  T++ E+ MV+   T  L EI+  ++EYF+KAA+AG  +S 
Sbjct: 301 PLPLRRRDERSEAGDSSSTVTAAAEMRMVIRHRT--LAEIVAAIEEYFVKAAEAGNGVSE 360

Query: 361 LLEVPTFSPQNKGGQ----AYSNGWSLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGC 420
           LLE           Q     Y +   LS     W S  KP  A  +   +  A  M +  
Sbjct: 361 LLEASRAQLDRNFRQLKKTVYHSNSLLSSLSSTWTS--KPPLAV-RYKLDTNALEMESME 420

Query: 421 GGNSSSHCSTVEKLYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKK 480
           G    SH ST+E+L AWEKKLYQEVK  E+ +IEHEKK+  L+ LE +  D  K +KTK 
Sbjct: 421 G---KSHGSTLERLLAWEKKLYQEVKARESVKIEHEKKLSTLQSLEYRGRDSTKLDKTKA 480

Query: 481 EVEKLESQMMVASQAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYESHQVQTHIV 540
            + KL+S ++V SQA  TTS+ I+++R+ EL PQL+EL   L+ MWRSM   H++Q  IV
Sbjct: 481 SINKLQSLIIVTSQAATTTSSAIVRVRDNELAPQLVELCFALLSMWRSMNHFHEIQNEIV 540

Query: 541 EQLKYLNIIPSAEPTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLF 600
           +Q++ L     AE TS++HR +T  LE  V  WH++F  L+K QRDY+++L GWL+L LF
Sbjct: 541 QQVRGLVDNSMAESTSDLHRLATRDLEAAVSAWHSNFNRLIKYQRDYIRALYGWLKLTLF 600

Query: 601 QISGN--PLLRTGQDSR-IYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEY 619
           Q+  N      T   SR + + C+EW  A+DR+PD  ASE IKSF+ VVH I  +QAEE 
Sbjct: 601 QVDSNIPQEAYTSLISRELTTFCDEWKQALDRLPDASASEAIKSFVNVVHVIYTKQAEEM 660

BLAST of HG10016352 vs. ExPASy Swiss-Prot
Match: Q93YU8 (Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 3.1e-76
Identity = 263/799 (32.92%), Postives = 384/799 (48.06%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAE- 60
           MGC  S+L+ E+ V RCK R+R MK+ V AR   +A+H+ Y RSLR TG+AL  F++ E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---------TYIRRPQQQQQQQNYHHHLPP-VLPSPTPRTPPPP---------------- 120
                     ++  P     +Q+    +PP   PSP P +  PP                
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 ----------PPPHIP----------------------MSPSSDTWTSITASPALPP--- 180
                     P P +P                      + PS+   ++ +A+P+      
Sbjct: 121 TSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSVW 180

Query: 181 ------PPPPPPS---------------------------STWDFWDP------------ 240
                 PP PP S                           S +DF+D             
Sbjct: 181 NWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFESMR 240

Query: 241 -FVPSTSRTVTEE----EWE-----ATTIASEAI-----------VTVTGAASS------ 300
             V   + T  EE    EWE     +TT +S+A            ++  G  S       
Sbjct: 241 NQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTVR 300

Query: 301 -----------APPPSVVSG-----FSKDTSST-------------ELAMVVSRNTKDLV 360
                      +P P V  G     + K   +T             ++ MVV    +DL 
Sbjct: 301 SNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRH--RDLK 360

Query: 361 EIIKELDEYFLKAADAGAQLSLLLEV------PTFSPQNKGGQAYSNGWSLSPSLRVWGS 420
           EII  + E F KAA +G Q+S +LE+       +FS   K      +   LS     W S
Sbjct: 361 EIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKK--TVIHSSSLLSNLSSTWTS 420

Query: 421 NTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEVKTAEATRIEHE 480
             KP  A  K   + TA +  N    +S S CST+++L AWEKKLY+E+K  E  +IEHE
Sbjct: 421 --KPPLAV-KYRIDTTALDQPN----SSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHE 480

Query: 481 KKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLL 540
           KK+ QL+  E K  D  K +KTK  + +L+S ++V SQA+ TTS  II+LR+ +L PQL+
Sbjct: 481 KKLSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLV 540

Query: 541 ELVKGLMCMWRSMYESHQVQTHIVEQLK-YLNIIPSAEPTSEIHRQSTLQLELEVQQWHA 600
           EL  G M MW+SM++ H+ Q  IVEQ++  +N     E TSE+HRQ+T  LE  V  WH+
Sbjct: 541 ELCHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHS 600

Query: 601 SFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDS-RIYSLCEEWNLAVDRIPDKV 619
           SF +L+K QRD++ S+  W +L L  +         ++    Y+ C+EW LA+DRIPD V
Sbjct: 601 SFSSLIKFQRDFIHSVHAWFKLTLLPVCQEDAANHHKEPLDAYAFCDEWKLALDRIPDTV 660

BLAST of HG10016352 vs. ExPASy TrEMBL
Match: A0A0A0KMI6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507110 PE=4 SV=1)

HSP 1 Score: 1154.0 bits (2984), Expect = 0.0e+00
Identity = 610/635 (96.06%), Postives = 616/635 (97.01%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRPQ  Q     HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRPQPHQ-----HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS Q KGGQ Y+NGW
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQKKGGQVYNNGW 240

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SL      WGSNTKPNDAFGKLNGE+TATNMGNG GGNS SHCSTVEKLYAWEKKLYQEV
Sbjct: 241 SL------WGSNTKPNDAFGKLNGELTATNMGNGYGGNSRSHCSTVEKLYAWEKKLYQEV 300

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK
Sbjct: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ
Sbjct: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL
Sbjct: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRA+ELRSLESKYV
Sbjct: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRATELRSLESKYV 540

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKV+NLRTKA+DEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 541 SHSMRECSASTRGRDPVQEKQNKVDNLRTKAQDEKQKHENLVSVTRSMTINNLQMSFPHV 600

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 FQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 624

BLAST of HG10016352 vs. ExPASy TrEMBL
Match: A0A5A7SHM1 (Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G001120 PE=4 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 610/635 (96.06%), Postives = 615/635 (96.85%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 69  MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 128

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRPQ  Q     HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 129 YIRRPQPHQ-----HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 188

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 189 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 248

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS Q KGGQ Y+NGW
Sbjct: 249 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQKKGGQVYNNGW 308

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SL      WGSNTKPNDAFGKLNGEI ATNMGNG GGNS SHCSTVEKLYAWEKKLYQEV
Sbjct: 309 SL------WGSNTKPNDAFGKLNGEIIATNMGNGYGGNSGSHCSTVEKLYAWEKKLYQEV 368

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK
Sbjct: 369 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 428

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ
Sbjct: 429 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 488

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL
Sbjct: 489 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 548

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRA+ELRSLESKYV
Sbjct: 549 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRATELRSLESKYV 608

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKV+NLRTKA+DEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 609 SHSMRECSASTRGRDPVQEKQNKVDNLRTKAQDEKQKHENLVSVTRSMTINNLQMSFPHV 668

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 669 FQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 692

BLAST of HG10016352 vs. ExPASy TrEMBL
Match: A0A1S3B2U3 (uncharacterized protein LOC103485147 OS=Cucumis melo OX=3656 GN=LOC103485147 PE=4 SV=1)

HSP 1 Score: 1152.9 bits (2981), Expect = 0.0e+00
Identity = 610/635 (96.06%), Postives = 615/635 (96.85%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRPQ  Q     HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRPQPHQ-----HHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFS Q KGGQ Y+NGW
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSSQKKGGQVYNNGW 240

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SL      WGSNTKPNDAFGKLNGEI ATNMGNG GGNS SHCSTVEKLYAWEKKLYQEV
Sbjct: 241 SL------WGSNTKPNDAFGKLNGEIIATNMGNGYGGNSGSHCSTVEKLYAWEKKLYQEV 300

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK
Sbjct: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ
Sbjct: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL
Sbjct: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRA+ELRSLESKYV
Sbjct: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRATELRSLESKYV 540

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKV+NLRTKA+DEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 541 SHSMRECSASTRGRDPVQEKQNKVDNLRTKAQDEKQKHENLVSVTRSMTINNLQMSFPHV 600

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 FQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 624

BLAST of HG10016352 vs. ExPASy TrEMBL
Match: A0A6J1GFM9 (nitrate regulatory gene2 protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453500 PE=4 SV=1)

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 593/637 (93.09%), Postives = 611/637 (95.92%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSA+HSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSATHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQ--NYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPP 120
           YIRRPQQQQQQQ  ++HHHLPPVLPSPTPRTPPPP P  +PMSPSSDTWTSITASPALPP
Sbjct: 61  YIRRPQQQQQQQQHHHHHHLPPVLPSPTPRTPPPPLP--VPMSPSSDTWTSITASPALPP 120

Query: 121 PPPPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSK 180
           PPPPPPSSTWDFWDPFVPS+SRTVTEEEWEATT ASEA+VTVTGAASSAPPPSVVSGFSK
Sbjct: 121 PPPPPPSSTWDFWDPFVPSSSRTVTEEEWEATTTASEAMVTVTGAASSAPPPSVVSGFSK 180

Query: 181 DTSSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSN 240
           DTSSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLS LLEVPTFS Q KGGQ Y+N
Sbjct: 181 DTSSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSSLLEVPTFSSQKKGGQVYNN 240

Query: 241 GWSLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQ 300
           GWSLSP+LR+WGSNT P +AFGKLNGEIT  NMGNGC GNS SHCSTVEKLYAWEKKLYQ
Sbjct: 241 GWSLSPTLRMWGSNTTPPNAFGKLNGEITTPNMGNGCAGNSGSHCSTVEKLYAWEKKLYQ 300

Query: 301 EVKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI 360
           EVK AEA R EHEKK+EQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI
Sbjct: 301 EVKNAEAIRTEHEKKLEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI 360

Query: 361 IKLREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQST 420
           +KLRE ELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQST
Sbjct: 361 VKLRETELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQST 420

Query: 421 LQLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEW 480
           LQLELEVQQWHASFCNLVKAQRDYVQSLT WLRLCL QISG PLLRTGQDSRIYSLCEEW
Sbjct: 421 LQLELEVQQWHASFCNLVKAQRDYVQSLTAWLRLCLLQISGTPLLRTGQDSRIYSLCEEW 480

Query: 481 NLAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESK 540
           NLAVDRIPD+VASEGIKSFLTVV AIVVQQAEE++QKKKADSASKEL+KRA++LRSLESK
Sbjct: 481 NLAVDRIPDRVASEGIKSFLTVVQAIVVQQAEEHKQKKKADSASKELEKRATQLRSLESK 540

Query: 541 YVSHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFP 600
           YVSHSMRECSASTRGRDPVQEKQNKVENLR KARDEKQKHENLVSVTRSMTINNLQMSFP
Sbjct: 541 YVSHSMRECSASTRGRDPVQEKQNKVENLRAKARDEKQKHENLVSVTRSMTINNLQMSFP 600

Query: 601 HVFQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           HVFQAMVGFSSVCMHAYEAVYNQTKN DQHEVKRLLP
Sbjct: 601 HVFQAMVGFSSVCMHAYEAVYNQTKNPDQHEVKRLLP 635

BLAST of HG10016352 vs. ExPASy TrEMBL
Match: A0A6J1IND0 (nitrate regulatory gene2 protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477994 PE=4 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 592/635 (93.23%), Postives = 609/635 (95.91%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSA+HSLYIRSLRGTGAALRQFSNAET
Sbjct: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSATHSLYIRSLRGTGAALRQFSNAET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSDTWTSITASPALPPPP 120
           YIRRP  QQQQQ++HHHLPPVLPSPTPRTPPPP P  IPMSPSSDTWTSITASPALPPPP
Sbjct: 61  YIRRP--QQQQQHHHHHLPPVLPSPTPRTPPPPLP--IPMSPSSDTWTSITASPALPPPP 120

Query: 121 PPPPSSTWDFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTGAASSAPPPSVVSGFSKDT 180
           PPPPSSTWDFWDPFVPS+SRTVTEEEWEATT ASEA+VTVTGAASSAPPPSVVSGFSKDT
Sbjct: 121 PPPPSSTWDFWDPFVPSSSRTVTEEEWEATTTASEAMVTVTGAASSAPPPSVVSGFSKDT 180

Query: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLEVPTFSPQNKGGQAYSNGW 240
           SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLS LLEVPTFS Q KGGQ Y+NGW
Sbjct: 181 SSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSSLLEVPTFSSQKKGGQVYNNGW 240

Query: 241 SLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEV 300
           SLSP+LR+WGSNT P +AFGKLNGEIT  NMGNGC GNS SHCSTVEKLYAWEKKLYQEV
Sbjct: 241 SLSPTLRMWGSNTTPPNAFGKLNGEITTPNMGNGCAGNSGSHCSTVEKLYAWEKKLYQEV 300

Query: 301 KTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIK 360
           K AEA RIEHEKK+EQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEI+K
Sbjct: 301 KNAEAIRIEHEKKLEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIVK 360

Query: 361 LREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAEPTSEIHRQSTLQ 420
           LRE ELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSA+PTSEIHRQSTLQ
Sbjct: 361 LRETELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSADPTSEIHRQSTLQ 420

Query: 421 LELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNL 480
           LELEVQQWHASFCNLVKAQRDYVQSLT WLRLCL QISG PLLRTGQDSRIYSLCEEWNL
Sbjct: 421 LELEVQQWHASFCNLVKAQRDYVQSLTAWLRLCLLQISGTPLLRTGQDSRIYSLCEEWNL 480

Query: 481 AVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKRASELRSLESKYV 540
           AVDRIPDKVASEGIKSFLTVV AIVVQQAEE++QKKKADSASKEL+KRA++LRSLESKYV
Sbjct: 481 AVDRIPDKVASEGIKSFLTVVQAIVVQQAEEHKQKKKADSASKELEKRATQLRSLESKYV 540

Query: 541 SHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600
           SHSMRECSASTRGRDPVQEKQNKVENLR KARDEKQKHENLVSVTRSMTINNLQMSFPHV
Sbjct: 541 SHSMRECSASTRGRDPVQEKQNKVENLRAKARDEKQKHENLVSVTRSMTINNLQMSFPHV 600

Query: 601 FQAMVGFSSVCMHAYEAVYNQTKNTDQHEVKRLLP 636
           FQAMVGFSSVCMHAYEAVYNQTKN D HEVKRLLP
Sbjct: 601 FQAMVGFSSVCMHAYEAVYNQTKNPDLHEVKRLLP 631

BLAST of HG10016352 vs. TAIR 10
Match: AT3G51290.2 (Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) )

HSP 1 Score: 696.4 bits (1796), Expect = 2.1e-200
Identity = 405/665 (60.90%), Postives = 493/665 (74.14%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCC SR++ +E VSRCKARKRY+K LVKARQ  S SH+LY+RSLR  G++L  FS+ ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSD--TWTSITAS----- 120
            +           + HH P   PSP+P  PPPP PP  P+SP S+  TWT+ T S     
Sbjct: 61  PL-----------HLHHNP---PSPSPPPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPP 120

Query: 121 PALPPPPPPPPSSTWDFWDPFVPSTSRTVTEEEWE---------ATTIASEAIVTVTGAA 180
           P  PPPPPPPPSSTWDFWDPF+P    + +EEEWE         AT   S+A VT     
Sbjct: 121 PPPPPPPPPPPSSTWDFWDPFIPPPPSS-SEEEWEEETTTATRTATGTGSDAAVTTAPTT 180

Query: 181 SSAPPPSVVSGFSKDTSST-----ELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSL 240
           ++    SVVSGFSKDT +T     ELA+VVSRN KDL+EIIKE+DEYFLKAAD+GA LS 
Sbjct: 181 ATPQASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSS 240

Query: 241 LLEVPT----FSPQNKGGQAYSNG---WSLSPSLRVWGSNTKPNDAFGKLNGEITATNMG 300
           LLE+ T    FS  +K G+ YS+     +L+P+   W     P+    KL+      N G
Sbjct: 241 LLEISTSITDFSGHSKSGKMYSSSNYECNLNPT-SFWTRGFAPS----KLS---EYRNAG 300

Query: 301 NGCGGNS--SSHCSTVEKLYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADYVKT 360
              GGN    SH STV++LYAWEKKLYQEVK AE+ +++HEKKVEQ+R+LE+KRA+YVKT
Sbjct: 301 GVIGGNCIVGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKT 360

Query: 361 EKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYESHQV 420
           EK KK+VEKLESQ+ V+SQAI++ S EIIKLRE ELYPQL+ELVKGLMCMWRSMYESHQV
Sbjct: 361 EKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKGLMCMWRSMYESHQV 420

Query: 421 QTHIVEQLKYLNIIPSAEPTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSLTGWL 480
           QTHIV+QLKYLN IPS EPTSE+HRQSTLQLELEVQQWH SFCNLVKAQRDY+QSLTGWL
Sbjct: 421 QTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWL 480

Query: 481 RLCLFQISGNPLLRTGQDSRIYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVVQQAE 540
           RL LFQ S NPL+R+  +S+IYS CEEW+LA+DRIPDKVASEGIKSFLT VH IV QQA+
Sbjct: 481 RLSLFQFSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQAD 540

Query: 541 EYRQKKKADSASKELDKRASELRSLESKYVSHSMRECSASTRGRDPVQEKQNKVENLRTK 600
           E++QKK+ +S  K+ +K+++ LR+LESKY  +S+ E    +R ++PV EK+ KVE L+ K
Sbjct: 541 EHKQKKRTESMLKDFEKKSASLRALESKYSPYSVPE----SRKKNPVIEKRVKVEMLKGK 600

Query: 601 ARDEKQKHENLVSVTRSMTINNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKNTDQHEV 636
           A +EK KHE  VSVTR+MT+NNLQM FPHVFQAMVGFSSVCM A+E+VYNQ K+  + + 
Sbjct: 601 AEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQE 638

BLAST of HG10016352 vs. TAIR 10
Match: AT3G51290.1 (Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) )

HSP 1 Score: 685.6 bits (1768), Expect = 3.7e-197
Identity = 407/667 (61.02%), Postives = 491/667 (73.61%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGCC SR++ +E VSRCKARKRY+K LVKARQ  S SH+LY+RSLR  G++L  FS+ ET
Sbjct: 1   MGCCQSRIDSKEIVSRCKARKRYLKHLVKARQTLSVSHALYLRSLRAVGSSLVHFSSKET 60

Query: 61  YIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPPPHIPMSPSSD--TWTSITAS----- 120
            +           + HH P   PSP+P  PPPP PP  P+SP S+  TWT+ T S     
Sbjct: 61  PL-----------HLHHNP---PSPSPPPPPPPRPPPPPLSPGSETTTWTTTTTSSVLPP 120

Query: 121 PALPPPPPPPPSSTWDFWDPFVPSTSRTVTEEEWE---------ATTIASEAIVTVTGAA 180
           P  PPPPPPPPSSTWDFWDPF+P    + +EEEWE         AT   S+A VT     
Sbjct: 121 PPPPPPPPPPPSSTWDFWDPFIPPPPSS-SEEEWEEETTTATRTATGTGSDAAVTTAPTT 180

Query: 181 SSAPPPSVVSGFSKDTSST-----ELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSL 240
           ++    SVVSGFSKDT +T     ELA+VVSRN KDL+EIIKE+DEYFLKAAD+GA LS 
Sbjct: 181 ATPQASSVVSGFSKDTMTTTTTGSELAVVVSRNGKDLMEIIKEVDEYFLKAADSGAPLSS 240

Query: 241 LLEVPT----FSPQNKGGQAYSNG---WSLSPSLRVWGSNTKPNDAFGKLNGEITATNMG 300
           LLE+ T    FS  +K G+ YS+     +L+P+   W     P+    KL+      N G
Sbjct: 241 LLEISTSITDFSGHSKSGKMYSSSNYECNLNPT-SFWTRGFAPS----KLS---EYRNAG 300

Query: 301 NGCGGNS--SSHCSTVEKLYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADYVKT 360
              GGN    SH STV++LYAWEKKLYQEVK AE+ +++HEKKVEQ+R+LE+KRA+YVKT
Sbjct: 301 GVIGGNCIVGSHSSTVDRLYAWEKKLYQEVKYAESIKMDHEKKVEQVRRLEMKRAEYVKT 360

Query: 361 EKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYESHQV 420
           EK KK+VEKLESQ+ V+SQAI++ S EIIKLRE ELYPQL+ELVKG      SMYESHQV
Sbjct: 361 EKAKKDVEKLESQLSVSSQAIQSASNEIIKLRETELYPQLVELVKG------SMYESHQV 420

Query: 421 QTHIVEQLKYLNIIPSAEPTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSLTGWL 480
           QTHIV+QLKYLN IPS EPTSE+HRQSTLQLELEVQQWH SFCNLVKAQRDY+QSLTGWL
Sbjct: 421 QTHIVQQLKYLNTIPSTEPTSELHRQSTLQLELEVQQWHHSFCNLVKAQRDYIQSLTGWL 480

Query: 481 RLCLFQISGNPLLRTGQDSRIYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVVQQAE 540
           RL LFQ S NPL+R+  +S+IYS CEEW+LA+DRIPDKVASEGIKSFLT VH IV QQA+
Sbjct: 481 RLSLFQFSKNPLVRSSYESKIYSFCEEWHLAIDRIPDKVASEGIKSFLTAVHGIVAQQAD 540

Query: 541 EYRQKKKADSASKELDKRASELRSLESKYVSHSMRECSASTRGRDPVQEKQNKVENLRTK 600
           E++QKK+ +S  K+ +K+++ LR+LESKY  +S+ E    +R ++PV EK+ KVE L+ K
Sbjct: 541 EHKQKKRTESMLKDFEKKSASLRALESKYSPYSVPE----SRKKNPVIEKRVKVEMLKGK 600

Query: 601 ARDEKQKHENLVSVTRSMTINNLQMSFPHVFQAMVGFSSVCMHAYEAVYNQTKN--TDQH 636
           A +EK KHE  VSVTR+MT+NNLQM FPHVFQAMVGFSSVCM A+E+VYNQ K+   DQ 
Sbjct: 601 AEEEKSKHEKSVSVTRAMTLNNLQMGFPHVFQAMVGFSSVCMQAFESVYNQAKSIGEDQE 634

BLAST of HG10016352 vs. TAIR 10
Match: AT1G02110.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 305.8 bits (782), Expect = 7.9e-83
Identity = 256/726 (35.26%), Postives = 371/726 (51.10%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAE- 60
           MGC  S+L+ E+ V RCK R+R MK  V AR   +A+HS Y RSLR TG+AL  F+  E 
Sbjct: 1   MGCTASKLDSEDAVRRCKERRRLMKDAVYARHHLAAAHSDYCRSLRLTGSALSSFAAGEP 60

Query: 61  ---------TYIRRPQQQQQQQNYHHHLPPVLPSPTPRTPPPPPP---PHI--PMSPSSD 120
                     ++R    Q   +    H P   P P    P P  P   PHI    SPSS 
Sbjct: 61  LSVSENTPAVFLRPSSSQDAPRVPSSHSPEPPPPPIRSKPKPTRPRRLPHILSDSSPSSS 120

Query: 121 TWTSI----------TASPA-----------LPPPPPP---------------PPS---- 180
             TS           + SP+            PP PP                PPS    
Sbjct: 121 PATSFYPTAHQNSTYSRSPSQASSVWNWENFYPPSPPDSEYFERKARQNHKHRPPSDYDA 180

Query: 181 ----------------------STW-DFWDPFVPSTSRTVTEEEWEATTIASEAIVTVTG 240
                                 S W D  D F  ++S   ++ + E  T  S + +    
Sbjct: 181 ETERSDHDYCHSRRDAAEEVHCSEWGDDHDRFTATSS---SDGDGEVETHVSRSGIEEEP 240

Query: 241 AASSAPPPSVVSGFSKDTSSTEL--AMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLL 300
                  P+        T+S++     +V R+ K+L EI+  + +YF KAA AG Q+S +
Sbjct: 241 VKQPHQDPNGKEHSDHVTTSSDCYKTKLVVRH-KNLKEILDAVQDYFDKAASAGDQVSAM 300

Query: 301 LEV------PTFSPQNK----GGQAYSN---GWSLSPSLRVWGSNTKPNDAFGKLNGEIT 360
           LE+       +FS   K        +SN    W+  P L V                ++ 
Sbjct: 301 LEIGRAELDRSFSKLRKTVYHSSSVFSNLSASWTSKPPLAV--------------KYKLD 360

Query: 361 ATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADY 420
           A+ + +  GG   S CST+++L AWEKKLY++VK  E  +IEHEKK+  L+  E K  D 
Sbjct: 361 ASTLNDEQGG-LKSLCSTLDRLLAWEKKLYEDVKAREGVKIEHEKKLSALQSQEYKGGDE 420

Query: 421 VKTEKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYES 480
            K +KTK  + +L+S ++V+S+A+ TTS  I++LR+ +L PQL+EL  GLM MW+SM+E 
Sbjct: 421 SKLDKTKTSITRLQSLIIVSSEAVLTTSNAILRLRDTDLVPQLVELCHGLMYMWKSMHEY 480

Query: 481 HQVQTHIVEQLK-YLNIIPSAEPTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSL 540
           H++Q +IV+Q++  +N     E TSE+HRQ T  LE  V  WH+SFC ++K QR+++ SL
Sbjct: 481 HEIQNNIVQQVRGLINQTERGESTSEVHRQVTRDLESAVSLWHSSFCRIIKFQREFICSL 540

Query: 541 TGWLRLCLFQISGNPLLRTGQDSRIYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVV 600
             W +L L  +S     +   DS  ++LCEEW  +++R+PD VASE IKSF+ VVH I +
Sbjct: 541 HAWFKLSLVPLSNGDPKKQRPDS--FALCEEWKQSLERVPDTVASEAIKSFVNVVHVISI 600

Query: 601 QQAEEYRQKKKADSASKELDKRASELRSLESKYVSHSMRECSASTRG----------RDP 623
           +QAEE + KK+ +SA KEL+K+AS LRS+E KY        + ST G          RDP
Sbjct: 601 KQAEEVKMKKRTESAGKELEKKASSLRSIERKYYQ------AYSTVGIGPGPEVLDSRDP 660

BLAST of HG10016352 vs. TAIR 10
Match: AT3G60320.1 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 287.7 bits (735), Expect = 2.2e-77
Identity = 263/799 (32.92%), Postives = 384/799 (48.06%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAE- 60
           MGC  S+L+ E+ V RCK R+R MK+ V AR   +A+H+ Y RSLR TG+AL  F++ E 
Sbjct: 1   MGCAASKLDNEDAVRRCKDRRRLMKEAVYARHHLAAAHADYCRSLRITGSALSSFASGEP 60

Query: 61  ---------TYIRRPQQQQQQQNYHHHLPP-VLPSPTPRTPPPP---------------- 120
                     ++  P     +Q+    +PP   PSP P +  PP                
Sbjct: 61  LSVSDQTPAVFLHTPPPPLSEQSPAKFVPPRFSPSPAPSSVYPPSTSPSVASSKQPSVMS 120

Query: 121 ----------PPPHIP----------------------MSPSSDTWTSITASPALPP--- 180
                     P P +P                      + PS+   ++ +A+P+      
Sbjct: 121 TSSNRRRKQQPKPRLPHILSESSPSSSPRSERSNFMPNLYPSAYQNSTYSATPSHASSVW 180

Query: 181 ------PPPPPPS---------------------------STWDFWDP------------ 240
                 PP PP S                           S +DF+D             
Sbjct: 181 NWENFYPPSPPDSEFFNRKAQEKKHNSDNRFNDEDTETVRSEYDFFDTRKQKQKQFESMR 240

Query: 241 -FVPSTSRTVTEE----EWE-----ATTIASEAI-----------VTVTGAASS------ 300
             V   + T  EE    EWE     +TT +S+A            ++  G  S       
Sbjct: 241 NQVEEETETEREEVQCSEWEDHDHYSTTSSSDAAEEEEEDDDRESISEVGTRSEFGSTVR 300

Query: 301 -----------APPPSVVSG-----FSKDTSST-------------ELAMVVSRNTKDLV 360
                      +P P V  G     + K   +T             ++ MVV    +DL 
Sbjct: 301 SNSMRRHHQQPSPMPQVYGGAEQSKYDKADDATISSGSYRGGGDIADMKMVVRH--RDLK 360

Query: 361 EIIKELDEYFLKAADAGAQLSLLLEV------PTFSPQNKGGQAYSNGWSLSPSLRVWGS 420
           EII  + E F KAA +G Q+S +LE+       +FS   K      +   LS     W S
Sbjct: 361 EIIDAIKENFDKAAASGEQVSQMLELGRAELDRSFSQLKK--TVIHSSSLLSNLSSTWTS 420

Query: 421 NTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEKLYAWEKKLYQEVKTAEATRIEHE 480
             KP  A  K   + TA +  N    +S S CST+++L AWEKKLY+E+K  E  +IEHE
Sbjct: 421 --KPPLAV-KYRIDTTALDQPN----SSKSLCSTLDRLLAWEKKLYEEIKAREGFKIEHE 480

Query: 481 KKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVASQAIETTSAEIIKLREIELYPQLL 540
           KK+ QL+  E K  D  K +KTK  + +L+S ++V SQA+ TTS  II+LR+ +L PQL+
Sbjct: 481 KKLSQLQSQEYKGEDEAKLDKTKASITRLQSLIIVTSQAVTTTSTAIIRLRDTDLVPQLV 540

Query: 541 ELVKGLMCMWRSMYESHQVQTHIVEQLK-YLNIIPSAEPTSEIHRQSTLQLELEVQQWHA 600
           EL  G M MW+SM++ H+ Q  IVEQ++  +N     E TSE+HRQ+T  LE  V  WH+
Sbjct: 541 ELCHGFMYMWKSMHQYHETQNSIVEQVRGLINRSGKGESTSELHRQATRDLESAVSSWHS 600

Query: 601 SFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQDS-RIYSLCEEWNLAVDRIPDKV 619
           SF +L+K QRD++ S+  W +L L  +         ++    Y+ C+EW LA+DRIPD V
Sbjct: 601 SFSSLIKFQRDFIHSVHAWFKLTLLPVCQEDAANHHKEPLDAYAFCDEWKLALDRIPDTV 660

BLAST of HG10016352 vs. TAIR 10
Match: AT2G34670.2 (Protein of unknown function (DUF630 and DUF632) )

HSP 1 Score: 281.2 bits (718), Expect = 2.1e-75
Identity = 235/708 (33.19%), Postives = 347/708 (49.01%), Query Frame = 0

Query: 1   MGCCYSRLEREETVSRCKARKRYMKQLVKARQAFSASHSLYIRSLRGTGAALRQFSNAET 60
           MGC  SR++ EE V  C+ RKR MK+L+  R  F+ +   Y+R+LR TG  LRQF+ +ET
Sbjct: 1   MGCAASRIDNEEKVLVCRQRKRLMKKLLGFRGEFADAQLAYLRALRNTGVTLRQFTESET 60

Query: 61  YIRRPQQQQQQQNYHHHLP-PVLPSPTPRTPPPPPPPHIPMSPSSDTWTSIT-------- 120
                  + +  +Y   LP P  P PT    PPPPPP  P   + +T   +         
Sbjct: 61  L------ELENTSYGLSLPLPPSPPPTLPPSPPPPPPFSPDLRNPETSHDLADEEEEGEN 120

Query: 121 -----ASPALPPPPPPPPSSTWDFWDPFV-------PSTSRTVT--------------EE 180
                 S A PPPP P   ++W+ W+PF        P+    VT              EE
Sbjct: 121 DGGNDGSGAAPPPPLP---NSWNIWNPFESLELHSHPNGDNVVTQVELKKKQQIQQAEEE 180

Query: 181 EWEAT------------------TIASEAIVTVTGAASSAP-------------PPSVVS 240
           +W  T                   ++   I  V+G     P               S+ S
Sbjct: 181 DWAETKSQFEEEDEQQEAGGTCLDLSVHQIEAVSGCNMKKPRRLKFKLGEVMDGNSSMTS 240

Query: 241 GFSKDTSSTELAMVVSRNTKDLVEIIKELDEYFLKAADAGAQLSLLLE------VPTFSP 300
              KD   T +     R T  L  II+ELD+YFLKA+    +++++++      V  F  
Sbjct: 241 CSGKDLEKTHVTDCRIRRT--LEGIIRELDDYFLKASGCEKEIAVIVDINSRDTVDPFRY 300

Query: 301 QNKGGQAYSNGWSLSPSLRVWGSNTKPNDAFGKLNGEITATNMGNGCGGNSSSHCSTVEK 360
           Q    +  S+    S     W S +       +L  + T +     C     +HCST+EK
Sbjct: 301 QETRRKRSSSAKVFSALSWSWSSKSL------QLGKDATTSGTVEPC--RPGAHCSTLEK 360

Query: 361 LYAWEKKLYQEVKTAEATRIEHEKKVEQLRKLELKRADYVKTEKTKKEVEKLESQMMVAS 420
           LY  EKKLYQ V+  E  ++EHE+K   L+K + +  D  K EK +  +E LE+++    
Sbjct: 361 LYTAEKKLYQLVRNKEIAKVEHERKSALLQKQDGETYDLSKMEKARLSLESLETEIQRLE 420

Query: 421 QAIETTSAEIIKLREIELYPQLLELVKGLMCMWRSMYESHQVQTHIVEQLKYLNIIPSAE 480
            +I TT + ++ L   ELYPQL+ L  GL  MW++M + HQVQ HI +QL +L   PS +
Sbjct: 421 DSITTTRSCLLNLINDELYPQLVALTSGLAQMWKTMLKCHQVQIHISQQLNHLPDYPSID 480

Query: 481 PTSEIHRQSTLQLELEVQQWHASFCNLVKAQRDYVQSLTGWLRLCLFQISGNPLLRTGQD 540
            +SE  RQ+  +LE EV  W+ SFC LV +QR+YV++L  W++L   ++S     R+   
Sbjct: 481 LSSEYKRQAVNELETEVTCWYNSFCKLVNSQREYVKTLCTWIQL-TDRLSNEDNQRSSLP 540

Query: 541 SRIYSLCEEWNLAVDRIPDKVASEGIKSFLTVVHAIVVQQAEEYRQKKKADSASKELDKR 600
                LC+EW L  +++PDKV SE IKSFL  + +I+ QQAEEY  ++K +   + L+K 
Sbjct: 541 VAARKLCKEWQLVFEKLPDKVTSEAIKSFLMSIKSIIHQQAEEYNLRRKCNKLERRLEKE 600

Query: 601 ASEL----RSLESKYVSHSMRECSASTRGRDPVQEKQNKVENLRTKARDEKQKHENLVSV 629
              L    R LE           S S   + P+  KQ K+E LR +   EK K+ N V V
Sbjct: 601 LISLAEIERRLEGILAMEEEEVSSTSLGSKHPLSIKQAKIEALRKRVDIEKTKYLNSVEV 660

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038880957.10.0e+0097.48protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X2 [Benincasa hispida][more]
XP_038880956.10.0e+0097.33protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X1 [Benincasa hispida][more]
XP_038880958.10.0e+0096.70protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X3 [Benincasa hispida][more]
XP_011658056.10.0e+0096.06protein ALTERED PHOSPHATE STARVATION RESPONSE 1 isoform X2 [Cucumis sativus][more]
KAA0025658.10.0e+0096.06Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-con... [more]
Match NameE-valueIdentityDescription
A0A178VBJ02.8e-20261.92Protein ALTERED PHOSPHATE STARVATION RESPONSE 1 OS=Arabidopsis thaliana OX=3702 ... [more]
Q9AQW18.5e-8235.09Protein ROLLING AND ERECT LEAF 2 OS=Oryza sativa subsp. japonica OX=39947 GN=REL... [more]
Q93YU83.1e-7632.92Nitrate regulatory gene2 protein OS=Arabidopsis thaliana OX=3702 GN=NRG2 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A0A0KMI60.0e+0096.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G507110 PE=4 SV=1[more]
A0A5A7SHM10.0e+0096.06Ras domain-containing protein/DUF632 domain-containing protein/DUF630 domain-con... [more]
A0A1S3B2U30.0e+0096.06uncharacterized protein LOC103485147 OS=Cucumis melo OX=3656 GN=LOC103485147 PE=... [more]
A0A6J1GFM90.0e+0093.09nitrate regulatory gene2 protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IND00.0e+0093.23nitrate regulatory gene2 protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT3G51290.22.1e-20060.90Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) [more]
AT3G51290.13.7e-19761.02Protein of unknown function (DUF630) ;Protein of unknown function (DUF632) [more]
AT1G02110.17.9e-8335.26Protein of unknown function (DUF630 and DUF632) [more]
AT3G60320.12.2e-7732.92Protein of unknown function (DUF630 and DUF632) [more]
AT2G34670.22.1e-7533.19Protein of unknown function (DUF630 and DUF632) [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 330..357
NoneNo IPR availableCOILSCoilCoilcoord: 518..538
NoneNo IPR availableCOILSCoilCoilcoord: 300..323
NoneNo IPR availableCOILSCoilCoilcoord: 557..584
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 80..101
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 113..129
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..132
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 63..77
NoneNo IPR availablePANTHERPTHR21450UNCHARACTERIZEDcoord: 1..623
NoneNo IPR availablePANTHERPTHR21450:SF23SERINE HYDROXYMETHYLTRANSFERASE, PUTATIVE (DUF632)-RELATEDcoord: 1..623
IPR006868Domain of unknown function DUF630PFAMPF04783DUF630coord: 1..59
e-value: 3.5E-22
score: 78.3
IPR006867Domain of unknown function DUF632PFAMPF04782DUF632coord: 196..502
e-value: 2.9E-103
score: 345.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016352.1HG10016352.1mRNA