HG10016326 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10016326 vs. NCBI nr
Match: XP_038880821.1 (thiol-disulfide oxidoreductase LTO1 [Benincasa hispida])

HSP 1 Score: 673.3 bits (1736), Expect = 1.2e-189
Identity = 346/379 (91.29%), Postives = 359/379 (94.72%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MACSATLFSLPLSFQ+PSTRQ+LSLFHHRYDSF HRKDSVRRR LVP+FCLSDG NQDTE
Sbjct: 1   MACSATLFSLPLSFQKPSTRQLLSLFHHRYDSFNHRKDSVRRRPLVPVFCLSDGANQDTE 60

Query: 61  PQPESTSAVSSSSSS--SSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPI 120
           PQ ESTSA SSSSS   SSSPSISS STYNLCAALGGIG VETAYLSYLKLT S AFCPI
Sbjct: 61  PQAESTSAASSSSSLSWSSSPSISSNSTYNLCAALGGIGLVETAYLSYLKLTGSTAFCPI 120

Query: 121 GGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGT 180
           GGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAIS QLA KKLPFGIDESDGRLVLLGT
Sbjct: 121 GGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISLQLAAKKLPFGIDESDGRLVLLGT 180

Query: 181 TTSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIII 240
           TTSMAAASAYFLYILNTKF GV+CSYCL SA+LSF+LFFATLKDFGLQESMRRLG QIII
Sbjct: 181 TTSMAAASAYFLYILNTKFSGVTCSYCLVSAVLSFSLFFATLKDFGLQESMRRLGSQIII 240

Query: 241 ASMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAF 300
           A +VFITLSTSYNGSLPISQSAAELDLPY+ETELTKPS+PLAI+LARHLQSIGAKMYGAF
Sbjct: 241 AGVVFITLSTSYNGSLPISQSAAELDLPYFETELTKPSSPLAISLARHLQSIGAKMYGAF 300

Query: 301 WCSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSG 360
           WCSHCVEQKEMFGREAAKLLDYVECFP+G+HKGT +EKACSEVGIEGFPTWVINGQVLSG
Sbjct: 301 WCSHCVEQKEMFGREAAKLLDYVECFPNGYHKGTMIEKACSEVGIEGFPTWVINGQVLSG 360

Query: 361 EKELSELAEISGFEPDGIS 378
           EKELSELAEISGFE DGIS
Sbjct: 361 EKELSELAEISGFEVDGIS 379

BLAST of HG10016326 vs. NCBI nr
Match: XP_008440888.1 (PREDICTED: thiol-disulfide oxidoreductase LTO1 [Cucumis melo])

HSP 1 Score: 631.3 bits (1627), Expect = 5.2e-177
Identity = 329/378 (87.04%), Postives = 348/378 (92.06%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MA  ATLFSL  SFQ PSTR++LS FHHRYDSFKHRK  VRRR L+P+  LSDGTNQDT 
Sbjct: 1   MASLATLFSLQFSFQTPSTRRLLSRFHHRYDSFKHRKYWVRRRPLLPVLSLSDGTNQDTR 60

Query: 61  PQPESTS-AVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPIG 120
           P+PESTS A SSSSSSSSSPSISS STY LCAALGGIGFVETAYLSYLKLTDSAAFCPIG
Sbjct: 61  PEPESTSTASSSSSSSSSSPSISSNSTYTLCAALGGIGFVETAYLSYLKLTDSAAFCPIG 120

Query: 121 GGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGTT 180
           GGSCD+VLNSDYAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDES GRLVLLGTT
Sbjct: 121 GGSCDDVLNSDYAAVFGVPLPLIGMVAYGLVGAISLQLAAKKLPFGIDESGGRLVLLGTT 180

Query: 181 TSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIIIA 240
           TSMAAASAYFLYILNT+F GV+CSYCL SA+LSF+LFFATLKDFGL ES RRLG QII+A
Sbjct: 181 TSMAAASAYFLYILNTQFSGVTCSYCLVSAVLSFSLFFATLKDFGLHESRRRLGSQIILA 240

Query: 241 SMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAFW 300
            +VF TLSTSY GSLPIS+SAAELDLPY+ETELTKPSTPLAI+LARHL SIGAKMYGAFW
Sbjct: 241 GIVFYTLSTSY-GSLPISRSAAELDLPYFETELTKPSTPLAISLARHLHSIGAKMYGAFW 300

Query: 301 CSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSGE 360
           CSHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EK CSEVGIEGFPTWVINGQVLSGE
Sbjct: 301 CSHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKICSEVGIEGFPTWVINGQVLSGE 360

Query: 361 KELSELAEISGFEPDGIS 378
           KELSELAEISGFE DGIS
Sbjct: 361 KELSELAEISGFEADGIS 377

BLAST of HG10016326 vs. NCBI nr
Match: XP_011658067.2 (thiol-disulfide oxidoreductase LTO1 [Cucumis sativus] >KGN49398.2 hypothetical protein Csa_003085 [Cucumis sativus])

HSP 1 Score: 627.9 bits (1618), Expect = 5.8e-176
Identity = 325/381 (85.30%), Postives = 347/381 (91.08%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MA  ATLFSL  SFQRPSTR+ LS FHHRYDSFKHRKD VRRR L+P+   SDGTNQDT+
Sbjct: 1   MASLATLFSLQFSFQRPSTRRFLSRFHHRYDSFKHRKDWVRRRTLLPVLSSSDGTNQDTQ 60

Query: 61  PQPES----TSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFC 120
           P+PES    +S+ SSSSSSSSSPSISS STY LCAALGGIGFVETAYLSYLKLTDSAAFC
Sbjct: 61  PEPESISTPSSSSSSSSSSSSSPSISSNSTYTLCAALGGIGFVETAYLSYLKLTDSAAFC 120

Query: 121 PIGGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLL 180
           PIGGG CDNVLNSDYAAVFGVPLP+IGMVAYGLVGA+S QLA KKLPFGIDES GRLVLL
Sbjct: 121 PIGGGGCDNVLNSDYAAVFGVPLPLIGMVAYGLVGAVSLQLAAKKLPFGIDESGGRLVLL 180

Query: 181 GTTTSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQI 240
           GTTTSMAAASAYFLYILNT+F GV+CSYCL SALLSF+LFFATLKDFGL ES RRL  QI
Sbjct: 181 GTTTSMAAASAYFLYILNTQFSGVTCSYCLVSALLSFSLFFATLKDFGLDESRRRLATQI 240

Query: 241 IIASMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYG 300
           I+A +VF TLSTSY GSLPIS+SAAELDLPY+ETELTKPSTPLAI+LA+HLQSIGAKMYG
Sbjct: 241 IMAGIVFFTLSTSY-GSLPISRSAAELDLPYFETELTKPSTPLAISLAKHLQSIGAKMYG 300

Query: 301 AFWCSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVL 360
           AFWCSHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EK CS+VGIEGFPTWVINGQVL
Sbjct: 301 AFWCSHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKVCSDVGIEGFPTWVINGQVL 360

Query: 361 SGEKELSELAEISGFEPDGIS 378
           SGEKELSELAEISGF  DGIS
Sbjct: 361 SGEKELSELAEISGFSADGIS 380

BLAST of HG10016326 vs. NCBI nr
Match: XP_023543882.1 (thiol-disulfide oxidoreductase LTO1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625.9 bits (1613), Expect = 2.2e-175
Identity = 320/377 (84.88%), Postives = 342/377 (90.72%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MACSATL  LP SF RP TRQ LS+FH RYDSFKH++D +RRR +VP+FCLS+GTNQDTE
Sbjct: 1   MACSATLPCLPCSFPRPCTRQNLSVFHQRYDSFKHQRDLIRRRLVVPVFCLSNGTNQDTE 60

Query: 61  PQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPIGG 120
           P+ EST      SS+SSSPSI S  T NLCAALGGIGFVETAYLSY+KLTDSAAFCPIGG
Sbjct: 61  PEAEST------SSASSSPSILSNLTDNLCAALGGIGFVETAYLSYVKLTDSAAFCPIGG 120

Query: 121 GSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGTTT 180
           GSCDNVLNSDYAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDESDGRLV+LG TT
Sbjct: 121 GSCDNVLNSDYAAVFGVPLPLIGMVAYGLVGAISLQLAAKKLPFGIDESDGRLVMLGATT 180

Query: 181 SMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIIIAS 240
           SMA ASAYFLYILNTKF GVSCSYCL SALLSF+LFFA LKDFGLQESMRRLG QI IAS
Sbjct: 181 SMATASAYFLYILNTKFSGVSCSYCLVSALLSFSLFFAVLKDFGLQESMRRLGSQIFIAS 240

Query: 241 MVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAFWC 300
           +VFITLSTSYNGSLP SQSAAELDLPY+ETELT  S  L+I+LARHL SIGAKMYGAFWC
Sbjct: 241 VVFITLSTSYNGSLPSSQSAAELDLPYFETELTNSSNALSISLARHLHSIGAKMYGAFWC 300

Query: 301 SHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSGEK 360
           SHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EKACSEVGIEGFPTWVINGQVLSGEK
Sbjct: 301 SHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKACSEVGIEGFPTWVINGQVLSGEK 360

Query: 361 ELSELAEISGFEPDGIS 378
           +LSELAEISGF+PDGIS
Sbjct: 361 DLSELAEISGFKPDGIS 371

BLAST of HG10016326 vs. NCBI nr
Match: KAG6603878.1 (Thiol-disulfide oxidoreductase LTO1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 623.6 bits (1607), Expect = 1.1e-174
Identity = 319/377 (84.62%), Postives = 341/377 (90.45%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MACSATL  LP SF RP TRQ LSLFH RYDSFKH++D +RRR +VP+FCLS+GTNQDTE
Sbjct: 31  MACSATLPCLPCSFPRPCTRQNLSLFHQRYDSFKHQRDLIRRRLVVPVFCLSNGTNQDTE 90

Query: 61  PQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPIGG 120
           P+ EST      SS+SSSPSI S  T NLCAALGGIGF+ETAYLSY+KLTDSAAFCPIGG
Sbjct: 91  PEAEST------SSASSSPSILSNLTDNLCAALGGIGFIETAYLSYVKLTDSAAFCPIGG 150

Query: 121 GSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGTTT 180
           GSCDNVLNSDYAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDESDGRLV+LG TT
Sbjct: 151 GSCDNVLNSDYAAVFGVPLPLIGMVAYGLVGAISLQLAAKKLPFGIDESDGRLVMLGATT 210

Query: 181 SMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIIIAS 240
           SMA ASAYFLYILNTKF GVSCSYCL SALLSF+LFFA LKDFGLQESMRRLG QI IAS
Sbjct: 211 SMATASAYFLYILNTKFSGVSCSYCLVSALLSFSLFFAVLKDFGLQESMRRLGSQIFIAS 270

Query: 241 MVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAFWC 300
           +VFITLSTSYNGSLP SQSAAELDLPY+ETELT  S  L+I+LARHL SIGAKMYGAFWC
Sbjct: 271 VVFITLSTSYNGSLPSSQSAAELDLPYFETELTNSSNALSISLARHLHSIGAKMYGAFWC 330

Query: 301 SHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSGEK 360
           SHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EKACSEVGIEGFPTWVINGQVLSGEK
Sbjct: 331 SHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKACSEVGIEGFPTWVINGQVLSGEK 390

Query: 361 ELSELAEISGFEPDGIS 378
           +LSELAEISGF+ DGIS
Sbjct: 391 DLSELAEISGFKADGIS 401

BLAST of HG10016326 vs. ExPASy Swiss-Prot
Match: Q8L540 (Thiol-disulfide oxidoreductase LTO1 OS=Arabidopsis thaliana OX=3702 GN=LTO1 PE=1 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 4.5e-107
Identity = 201/336 (59.82%), Postives = 252/336 (75.00%), Query Frame = 0

Query: 39  SVRRRHLVPIFCLSDGTNQDTEPQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGF 98
           S RR   +PI C S       +  P   S  SSSSSS+S  S S++STYN    +GGIG 
Sbjct: 35  SDRRFPAIPIKCSSSEPENGEDSAP---SLSSSSSSSTSEVSTSNSSTYNWYTGIGGIGM 94

Query: 99  VETAYLSYLKLTDSAAFCPIGGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLA 158
           ++TAYL+YLK+T S AFCPIGGG+C +VLNSDYA VFGVPLPVIG V YG+V A+S +L 
Sbjct: 95  LDTAYLTYLKVTGSDAFCPIGGGTCGDVLNSDYAVVFGVPLPVIGFVMYGVVTALSAELG 154

Query: 159 VKKLPFGIDESDGRLVLLGTTTSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFA 218
              LPFGI +S+GR  L G TT+MA+ASAYFLYIL+TK  G SC YCL SA LSF+LFF 
Sbjct: 155 EGNLPFGISKSNGRFALFGITTAMASASAYFLYILSTKLSGSSCLYCLVSAFLSFSLFFL 214

Query: 219 TLKDFGLQESMRRLGPQIIIASMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTP 278
           ++KD  LQE  + +G QI +A +V  +L+ SY+ + PI   + +++LPY+ TE++  S+P
Sbjct: 215 SVKDVKLQEIQQVVGLQICLAIIVVASLTASYSTAQPIPSRSGDIELPYFRTEISSSSSP 274

Query: 279 LAIALARHLQSIGAKMYGAFWCSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKAC 338
            AIALA+HL SIGAKMYGAFWCSHC+EQKEMFGREAAK L+YVECFP G+ KGTK+ KAC
Sbjct: 275 YAIALAKHLNSIGAKMYGAFWCSHCLEQKEMFGREAAKELNYVECFPDGYKKGTKILKAC 334

Query: 339 SEVGIEGFPTWVINGQVLSGEKELSELAEISGFEPD 375
           ++  IEGFPTW+IN +VLSGE EL+ELAE++GF  D
Sbjct: 335 ADAAIEGFPTWIINDKVLSGEIELAELAEMTGFSLD 367

BLAST of HG10016326 vs. ExPASy Swiss-Prot
Match: Q2JJF6 (Vitamin K epoxide reductase homolog OS=Synechococcus sp. (strain JA-2-3B'a(2-13)) OX=321332 GN=CYB_2278 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.2e-36
Identity = 97/284 (34.15%), Postives = 144/284 (50.70%), Query Frame = 0

Query: 89  LCAALGGIGFVETAYLSYLKLTDS-AAFCPIGGGSCDNVLNSDYAAVFGVPLPVIGMVAY 148
           + A L G+G + TAYL+Y KLT+  AAFC  G G CD VL+S +A   G+P   +G++  
Sbjct: 22  ILAILAGLGSLLTAYLTYTKLTEQPAAFC-TGDGGCDLVLSSRWAEFLGIPTAAVGLL-- 81

Query: 149 GLVGAISFQLAVKKLPFGIDESDGRLVLLGTTTSMAAASAYFLYILNTKFPGVSCSYCLA 208
           G +G ++  +    LP  + +      L G  ++M A   Y LY++        C YC  
Sbjct: 82  GFLGVLALAVLPDGLP--LVKRWRWPALFGLVSAMTAFEMYMLYLMVAVLRQF-CMYCTT 141

Query: 209 SALLSFTLFFATLKDFGLQESMRRLGPQIIIASMVFITLSTSYNGSLPISQSAAELDLPY 268
           + +L   L   T+      +  +     I++A +  +T    Y   +P            
Sbjct: 142 AIILVAGLGLVTVLGHRWLDGGKLAFSYILVAFLTLVTTIGVYANQVP------------ 201

Query: 269 YETELTKPSTPLAIALARHLQSIGAKMYGAFWCSHCVEQKEMFGREAAKLLDYVECFPSG 328
                  P +PLA+ LA HL+ IG  MYGA+WC HC +QKE+FG  A   + YVEC P+G
Sbjct: 202 -------PPSPLAVGLAAHLRQIGGTMYGAYWCPHCQDQKELFG-AAFDQVPYVECSPNG 261

Query: 329 FHKGTKMEKACSEVGIEGFPTWVINGQVLSGEKELSELAEISGF 372
              GT   + C+E GI  +PTW+ING+  +G + L  LA  SG+
Sbjct: 262 --PGTPQAQECTEAGITSYPTWIINGRTYTGVRSLEALAVASGY 277

BLAST of HG10016326 vs. ExPASy TrEMBL
Match: A0A1S3B271 (thiol-disulfide oxidoreductase LTO1 OS=Cucumis melo OX=3656 GN=LOC103485174 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.5e-177
Identity = 329/378 (87.04%), Postives = 348/378 (92.06%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MA  ATLFSL  SFQ PSTR++LS FHHRYDSFKHRK  VRRR L+P+  LSDGTNQDT 
Sbjct: 1   MASLATLFSLQFSFQTPSTRRLLSRFHHRYDSFKHRKYWVRRRPLLPVLSLSDGTNQDTR 60

Query: 61  PQPESTS-AVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPIG 120
           P+PESTS A SSSSSSSSSPSISS STY LCAALGGIGFVETAYLSYLKLTDSAAFCPIG
Sbjct: 61  PEPESTSTASSSSSSSSSSPSISSNSTYTLCAALGGIGFVETAYLSYLKLTDSAAFCPIG 120

Query: 121 GGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGTT 180
           GGSCD+VLNSDYAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDES GRLVLLGTT
Sbjct: 121 GGSCDDVLNSDYAAVFGVPLPLIGMVAYGLVGAISLQLAAKKLPFGIDESGGRLVLLGTT 180

Query: 181 TSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIIIA 240
           TSMAAASAYFLYILNT+F GV+CSYCL SA+LSF+LFFATLKDFGL ES RRLG QII+A
Sbjct: 181 TSMAAASAYFLYILNTQFSGVTCSYCLVSAVLSFSLFFATLKDFGLHESRRRLGSQIILA 240

Query: 241 SMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAFW 300
            +VF TLSTSY GSLPIS+SAAELDLPY+ETELTKPSTPLAI+LARHL SIGAKMYGAFW
Sbjct: 241 GIVFYTLSTSY-GSLPISRSAAELDLPYFETELTKPSTPLAISLARHLHSIGAKMYGAFW 300

Query: 301 CSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSGE 360
           CSHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EK CSEVGIEGFPTWVINGQVLSGE
Sbjct: 301 CSHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKICSEVGIEGFPTWVINGQVLSGE 360

Query: 361 KELSELAEISGFEPDGIS 378
           KELSELAEISGFE DGIS
Sbjct: 361 KELSELAEISGFEADGIS 377

BLAST of HG10016326 vs. ExPASy TrEMBL
Match: A0A0A0KMN0 (VKc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G507390 PE=3 SV=1)

HSP 1 Score: 626.3 bits (1614), Expect = 8.2e-176
Identity = 325/385 (84.42%), Postives = 347/385 (90.13%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MA  ATLFSL  SFQRPSTR+ LS FHHRYDSFKHRKD VRRR L+P+   SDGTNQDT+
Sbjct: 1   MASLATLFSLQFSFQRPSTRRFLSRFHHRYDSFKHRKDWVRRRTLLPVLSSSDGTNQDTQ 60

Query: 61  PQPE--------STSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDS 120
           P+PE        S+S+ SSSSSSSSSPSISS STY LCAALGGIGFVETAYLSYLKLTDS
Sbjct: 61  PEPESISTPSSSSSSSSSSSSSSSSSPSISSNSTYTLCAALGGIGFVETAYLSYLKLTDS 120

Query: 121 AAFCPIGGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGR 180
           AAFCPIGGG CDNVLNSDYAAVFGVPLP+IGMVAYGLVGA+S QLA KKLPFGIDES GR
Sbjct: 121 AAFCPIGGGGCDNVLNSDYAAVFGVPLPLIGMVAYGLVGAVSLQLAAKKLPFGIDESGGR 180

Query: 181 LVLLGTTTSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRL 240
           LVLLGTTTSMAAASAYFLYILNT+F GV+CSYCL SALLSF+LFFATLKDFGL ES RRL
Sbjct: 181 LVLLGTTTSMAAASAYFLYILNTQFSGVTCSYCLVSALLSFSLFFATLKDFGLDESRRRL 240

Query: 241 GPQIIIASMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGA 300
             QII+A +VF TLSTSY GSLPIS+SAAELDLPY+ETELTKPSTPLAI+LA+HLQSIGA
Sbjct: 241 ATQIIMAGIVFFTLSTSY-GSLPISRSAAELDLPYFETELTKPSTPLAISLAKHLQSIGA 300

Query: 301 KMYGAFWCSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVIN 360
           KMYGAFWCSHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EK CS+VGIEGFPTWVIN
Sbjct: 301 KMYGAFWCSHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKVCSDVGIEGFPTWVIN 360

Query: 361 GQVLSGEKELSELAEISGFEPDGIS 378
           GQVLSGEKELSELAEISGF  DGIS
Sbjct: 361 GQVLSGEKELSELAEISGFSADGIS 384

BLAST of HG10016326 vs. ExPASy TrEMBL
Match: A0A6J1IRN7 (thiol-disulfide oxidoreductase LTO1 OS=Cucurbita maxima OX=3661 GN=LOC111477850 PE=3 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 2.0e-174
Identity = 318/377 (84.35%), Postives = 341/377 (90.45%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MACSATL  LP SF RP TRQ LSLFH R DSFKH++D +RRR +VP+FCLS+GTNQDTE
Sbjct: 1   MACSATLPCLPCSFTRPCTRQNLSLFHQRCDSFKHQRDLIRRRLVVPVFCLSNGTNQDTE 60

Query: 61  PQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPIGG 120
           P+ EST     SS+SSSSPSI S  T NLCAALGG+GFVETAYLSY+KLTDSAAFCPIGG
Sbjct: 61  PEAEST-----SSASSSSPSILSNLTDNLCAALGGVGFVETAYLSYVKLTDSAAFCPIGG 120

Query: 121 GSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGTTT 180
           GSCDNVLNSDYAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDESDGRLV+ G TT
Sbjct: 121 GSCDNVLNSDYAAVFGVPLPLIGMVAYGLVGAISLQLAAKKLPFGIDESDGRLVMFGATT 180

Query: 181 SMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIIIAS 240
           SMA ASAYFLYILNTKF GV+CSYCL SALLSF+LFFA LKDFGLQESMRRLG QI IAS
Sbjct: 181 SMATASAYFLYILNTKFSGVTCSYCLVSALLSFSLFFAVLKDFGLQESMRRLGSQIFIAS 240

Query: 241 MVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAFWC 300
           +VFITLSTSYNGSLP SQSAAELDLPY+ETELT  S  L+I+LARHL SIGAKMYGAFWC
Sbjct: 241 VVFITLSTSYNGSLPSSQSAAELDLPYFETELTNSSNALSISLARHLHSIGAKMYGAFWC 300

Query: 301 SHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSGEK 360
           SHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EKACSEVGIEGFPTWVINGQVLSGEK
Sbjct: 301 SHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKACSEVGIEGFPTWVINGQVLSGEK 360

Query: 361 ELSELAEISGFEPDGIS 378
           +LSELAEISGF+PDGIS
Sbjct: 361 DLSELAEISGFKPDGIS 372

BLAST of HG10016326 vs. ExPASy TrEMBL
Match: A0A6J1GGF8 (thiol-disulfide oxidoreductase LTO1 OS=Cucurbita moschata OX=3662 GN=LOC111453687 PE=3 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 1.3e-173
Identity = 318/377 (84.35%), Postives = 340/377 (90.19%), Query Frame = 0

Query: 1   MACSATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQDTE 60
           MACSATL  LP SF RP +RQ LSLFH RYDSFKH++D +R R +VP+FCLS+GTNQDTE
Sbjct: 1   MACSATLPCLPCSFPRPCSRQNLSLFHQRYDSFKHQRDLIRGRLVVPVFCLSNGTNQDTE 60

Query: 61  PQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPIGG 120
           P+ EST      SS+SSSPSI S  T NLCAALGGIGFVETAYLSY+KLTDSAAFCPIGG
Sbjct: 61  PEAEST------SSASSSPSILSNLTDNLCAALGGIGFVETAYLSYVKLTDSAAFCPIGG 120

Query: 121 GSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGTTT 180
           GSCDNVLNSDYAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDESDGRLV+LG TT
Sbjct: 121 GSCDNVLNSDYAAVFGVPLPLIGMVAYGLVGAISLQLAEKKLPFGIDESDGRLVMLGATT 180

Query: 181 SMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIIIAS 240
           SMA ASAYFLYILNTKF GVSCSYCL SALLSF+LFFA LKDFGLQESMRRLG QI IAS
Sbjct: 181 SMATASAYFLYILNTKFSGVSCSYCLVSALLSFSLFFAVLKDFGLQESMRRLGSQIFIAS 240

Query: 241 MVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAFWC 300
           +VFITLSTSYNGSLP SQSAAELDLPY+ETELT  S  L+I+LARHL SIGAKMYGAFWC
Sbjct: 241 VVFITLSTSYNGSLPSSQSAAELDLPYFETELTNSSNALSISLARHLHSIGAKMYGAFWC 300

Query: 301 SHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSGEK 360
           SHCVEQKEMFGREAAKLLDYVECFP+G+HKGTK+EKACSEVGIEGFPTWVINGQVLSGEK
Sbjct: 301 SHCVEQKEMFGREAAKLLDYVECFPNGYHKGTKIEKACSEVGIEGFPTWVINGQVLSGEK 360

Query: 361 ELSELAEISGFEPDGIS 378
           +LSELAEISGF+ DGIS
Sbjct: 361 DLSELAEISGFKADGIS 371

BLAST of HG10016326 vs. ExPASy TrEMBL
Match: A0A6J1BV35 (thiol-disulfide oxidoreductase LTO1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005967 PE=3 SV=1)

HSP 1 Score: 602.4 bits (1552), Expect = 1.3e-168
Identity = 307/379 (81.00%), Postives = 340/379 (89.71%), Query Frame = 0

Query: 1   MAC--SATLFSLPLSFQRPSTRQVLSLFHHRYDSFKHRKDSVRRRHLVPIFCLSDGTNQD 60
           MAC  SA L SLPLSFQRPS RQ LSLFHHRYDSF + K  V+RR LVP+FCLS GTNQD
Sbjct: 1   MACSTSAALLSLPLSFQRPSPRQFLSLFHHRYDSFNYPKFWVQRRLLVPVFCLSGGTNQD 60

Query: 61  TEPQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGFVETAYLSYLKLTDSAAFCPI 120
           TEP+ ++T      SSSS SPSISS STYNLCAALGGIGFVETAYLSYLKL ++AAFCPI
Sbjct: 61  TEPEADTTPL---DSSSSFSPSISSNSTYNLCAALGGIGFVETAYLSYLKLANAAAFCPI 120

Query: 121 GGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLAVKKLPFGIDESDGRLVLLGT 180
           GGGSCD+VLNS+YAAVFGVPLP+IGMVAYGLVGAIS QLA KKLPFGIDESDGRLVLLGT
Sbjct: 121 GGGSCDDVLNSEYAAVFGVPLPLIGMVAYGLVGAISLQLAAKKLPFGIDESDGRLVLLGT 180

Query: 181 TTSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFATLKDFGLQESMRRLGPQIII 240
           TTSMAAASAYFLY+L++KF GVSCSYCL SALLS +LFFAT+K+FG QE  RR+G QI +
Sbjct: 181 TTSMAAASAYFLYVLSSKFSGVSCSYCLISALLSSSLFFATVKEFGFQEITRRVGFQIFV 240

Query: 241 ASMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTPLAIALARHLQSIGAKMYGAF 300
           AS+VF+TLS SYN S P+SQSAAELDLPYY+ E+ KPS+PLAI+LARHLQSIGAKMYGAF
Sbjct: 241 ASLVFVTLSASYNSSFPVSQSAAELDLPYYQVEIAKPSSPLAISLARHLQSIGAKMYGAF 300

Query: 301 WCSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKACSEVGIEGFPTWVINGQVLSG 360
           WCSHC+EQKEMFGREAAK+LDYVECFP+G+HKGTK+EKAC+E GIEGFP+WVINGQVLSG
Sbjct: 301 WCSHCLEQKEMFGREAAKILDYVECFPNGYHKGTKIEKACTEAGIEGFPSWVINGQVLSG 360

Query: 361 EKELSELAEISGFEPDGIS 378
           EKELSELAEISGFE DGIS
Sbjct: 361 EKELSELAEISGFELDGIS 376

BLAST of HG10016326 vs. TAIR 10
Match: AT4G35760.1 (NAD(P)H dehydrogenase (quinone)s )

HSP 1 Score: 389.4 bits (999), Expect = 3.2e-108
Identity = 201/336 (59.82%), Postives = 252/336 (75.00%), Query Frame = 0

Query: 39  SVRRRHLVPIFCLSDGTNQDTEPQPESTSAVSSSSSSSSSPSISSTSTYNLCAALGGIGF 98
           S RR   +PI C S       +  P   S  SSSSSS+S  S S++STYN    +GGIG 
Sbjct: 35  SDRRFPAIPIKCSSSEPENGEDSAP---SLSSSSSSSTSEVSTSNSSTYNWYTGIGGIGM 94

Query: 99  VETAYLSYLKLTDSAAFCPIGGGSCDNVLNSDYAAVFGVPLPVIGMVAYGLVGAISFQLA 158
           ++TAYL+YLK+T S AFCPIGGG+C +VLNSDYA VFGVPLPVIG V YG+V A+S +L 
Sbjct: 95  LDTAYLTYLKVTGSDAFCPIGGGTCGDVLNSDYAVVFGVPLPVIGFVMYGVVTALSAELG 154

Query: 159 VKKLPFGIDESDGRLVLLGTTTSMAAASAYFLYILNTKFPGVSCSYCLASALLSFTLFFA 218
              LPFGI +S+GR  L G TT+MA+ASAYFLYIL+TK  G SC YCL SA LSF+LFF 
Sbjct: 155 EGNLPFGISKSNGRFALFGITTAMASASAYFLYILSTKLSGSSCLYCLVSAFLSFSLFFL 214

Query: 219 TLKDFGLQESMRRLGPQIIIASMVFITLSTSYNGSLPISQSAAELDLPYYETELTKPSTP 278
           ++KD  LQE  + +G QI +A +V  +L+ SY+ + PI   + +++LPY+ TE++  S+P
Sbjct: 215 SVKDVKLQEIQQVVGLQICLAIIVVASLTASYSTAQPIPSRSGDIELPYFRTEISSSSSP 274

Query: 279 LAIALARHLQSIGAKMYGAFWCSHCVEQKEMFGREAAKLLDYVECFPSGFHKGTKMEKAC 338
            AIALA+HL SIGAKMYGAFWCSHC+EQKEMFGREAAK L+YVECFP G+ KGTK+ KAC
Sbjct: 275 YAIALAKHLNSIGAKMYGAFWCSHCLEQKEMFGREAAKELNYVECFPDGYKKGTKILKAC 334

Query: 339 SEVGIEGFPTWVINGQVLSGEKELSELAEISGFEPD 375
           ++  IEGFPTW+IN +VLSGE EL+ELAE++GF  D
Sbjct: 335 ADAAIEGFPTWIINDKVLSGEIELAELAEMTGFSLD 367