Homology
BLAST of HG10016308 vs. NCBI nr
Match:
XP_038882615.1 (probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida])
HSP 1 Score: 1156.4 bits (2990), Expect = 0.0e+00
Identity = 559/587 (95.23%), Postives = 574/587 (97.79%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSVLSLNRALWT +FPSNRRGFSDLPESWKSM GLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVLSLNRALWTRNFPSNRRGFSDLPESWKSMMGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAKVYRDT SIVYGS SFTWEETY+RCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKVYRDTPSIVYGSMSFTWEETYDRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPM GA+LCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLE+GDSEP
Sbjct: 121 LHFAVPMVGAILCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLERGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPASLSSNVYEYESLIASGSC+FE+RKPKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCDFEVRKPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
PK IFE IALHNVTHMAGAPTVLNMIVNS VSDRRLLPNKVDVLTGG+PPPPQVLAKME
Sbjct: 301 LPKTIFENIALHNVTHMAGAPTVLNMIVNSQVSDRRLLPNKVDVLTGGSPPPPQVLAKME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWDGLP +ERSRI+ARQGVHHVGLEEVDVRDPVT
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPSNERSRIRARQGVHHVGLEEVDVRDPVT 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVPADGKT+GEIMFRGNTVMSGYFKNQKATEE+F+GGWFHSGDAAVKH DHYIEVKDR
Sbjct: 421 MASVPADGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHLDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCNVTAQQ 540
LKDVIISGGENISTVEVETVLFGHP+VLEAAVVARPDDHWGETPCAFVVLKEGCNVTAQQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFGHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNVTAQQ 540
Query: 541 LINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
LI+YCRDRLPHYMAPRSIIFQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 LIDYCRDRLPHYMAPRSIIFQDLPKTSTGKVQKFILRERAKAMGSLS 587
BLAST of HG10016308 vs. NCBI nr
Match:
XP_004134830.2 (probable acyl-activating enzyme 2 isoform X1 [Cucumis sativus])
HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 552/588 (93.88%), Postives = 574/588 (97.62%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSV+SLNRALWTW+FPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVVSLNRALWTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAK YRDTTSIVYGS SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPMAGAVLCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAI+LLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSCEFE+R+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
SPKAIFEKIALHN THMA APTVLNMI NSPVSDRRLLPNKVDVLTGG+PPPPQVLA+ME
Sbjct: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWD LP DE+SRI+ARQG+HHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVP DGKT+GEIMFRGNTVMSGYFKNQKATEE+F GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFMGGWFHSGDAAVKHPDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540
LKDVIISGGENISTVEVET+LF HP+VLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI+YCRDRLPHYMAPR+I+FQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588
BLAST of HG10016308 vs. NCBI nr
Match:
XP_008440901.1 (PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo])
HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 555/588 (94.39%), Postives = 573/588 (97.45%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSVLSLNRAL TW+FPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAK YRDTTSIVYGS SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPMAGAVLCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAIQLLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFE+R+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
SPKAIFE IALHN THMA APTVLNMIVNSPVSDRRLLPNKVDVLTGG+PPPPQV A+ME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWD LP DE+SRI+ARQGVHHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVP DGKT+GEIMFRGNTVMSGYFKNQKATEE+F+GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540
LKDVIISGGENISTVEVETVLF HP+VLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI+YCRDRLPHYMAPRSI+FQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588
BLAST of HG10016308 vs. NCBI nr
Match:
KAA0025615.1 (putative acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo var. makuwa] >TYK12490.1 putative acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo var. makuwa])
HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 554/588 (94.22%), Postives = 572/588 (97.28%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSVLSLNRAL TW+FPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAK YRDTTSIVYGS SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPMAGAVLCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAIQLLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFE+R+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGM SMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMRSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
SPKAIFE IALHN THMA APTVLNMIVNSPVSDRRLLPNKVDVLTGG+PPPPQV A+ME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWD LP DE+SRI+ARQGVHHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVP DGKT+GEIMFRGNTVMSGYFKNQKATEE+F+GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540
LKDVIISGGENISTVEVETVLF HP+VLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI+YCRDRLPHYMAPRSI+FQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588
BLAST of HG10016308 vs. NCBI nr
Match:
XP_022132791.1 (probable acyl-activating enzyme 1, peroxisomal isoform X1 [Momordica charantia])
HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 533/592 (90.03%), Postives = 563/592 (95.10%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALW--TW--DFPSNRRGFSDLPESWKSMTGLVRCPANDVPLS 60
MNRFLQ L RSV S NR LW W DFPS+RRGF+++P+SWKSM GLVRCPANDVPLS
Sbjct: 1 MNRFLQNQLLRSVCSFNRPLWNRNWNLDFPSSRRGFANVPDSWKSMAGLVRCPANDVPLS 60
Query: 61 PISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVP 120
P+SFLERTAKVYRDTTS+VYGS SFTWEETYNRCLKLASA++QLGIS GQVVATLAPNVP
Sbjct: 61 PLSFLERTAKVYRDTTSVVYGSVSFTWEETYNRCLKLASAISQLGISPGQVVATLAPNVP 120
Query: 121 AMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQG 180
AMYELHFAVPMAGAVLCTLNARHDSSMVS LLRHSEAKIIFVD+QLFEVACEAIQLL QG
Sbjct: 121 AMYELHFAVPMAGAVLCTLNARHDSSMVSALLRHSEAKIIFVDFQLFEVACEAIQLLAQG 180
Query: 181 DSEPPKLVLILDSEHGSSPAS-LSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSG 240
DSE PKLVLILD+EHG SPA+ LSSNV EYESLIASGSCEFE++KPKSEWDPISINYTSG
Sbjct: 181 DSESPKLVLILDAEHGLSPANPLSSNVCEYESLIASGSCEFEVQKPKSEWDPISINYTSG 240
Query: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNL 300
TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCL WGVAAQGGTN+
Sbjct: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLAWGVAAQGGTNI 300
Query: 301 CLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQV 360
CLRKVSPKAIFEKIAL NVTHMA APTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQ+
Sbjct: 301 CLRKVSPKAIFEKIALQNVTHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQI 360
Query: 361 LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDV 420
LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWD LP DER+RI+ARQGVHHVGL+EVDV
Sbjct: 361 LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDSLPSDERARIRARQGVHHVGLQEVDV 420
Query: 421 RDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYI 480
RDPVTMA VPADGKT+GEIMFRGNTVMSGYFKNQKATEE+FRGGWFHSGDAAVKHPDHYI
Sbjct: 421 RDPVTMAGVPADGKTMGEIMFRGNTVMSGYFKNQKATEEAFRGGWFHSGDAAVKHPDHYI 480
Query: 481 EVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN 540
EVKDRLKD++ISGGENISTVEVET+LFGHPAVLEAAVV RPD+HWGETPCAFV LKEGCN
Sbjct: 481 EVKDRLKDIVISGGENISTVEVETILFGHPAVLEAAVVGRPDNHWGETPCAFVTLKEGCN 540
Query: 541 VTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
+TAQQLI+YCRD LPHYMAPRSIIFQ+LPKTSTGK+QKFILR+RAKAMGSLS
Sbjct: 541 ITAQQLIDYCRDHLPHYMAPRSIIFQDLPKTSTGKIQKFILRDRAKAMGSLS 592
BLAST of HG10016308 vs. ExPASy Swiss-Prot
Match:
Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)
HSP 1 Score: 796.2 bits (2055), Expect = 2.5e-229
Identity = 392/602 (65.12%), Postives = 469/602 (77.91%), Query Frame = 0
Query: 3 RFLQT-HLFRSVLSLNRALWTWDFP----SNRRGFSD--LPESWKSMTGLVRCPANDVPL 62
RFL T FR + LW P SN GF D PESW+++ GL+R PAN PL
Sbjct: 2 RFLLTKRAFRIFNPRFQRLWLTSSPFSSTSNSGGFPDDSEPESWRTIEGLLRSPANFSPL 61
Query: 63 SPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNV 122
SPI+FLER+AKVYRD TS+V+GS TW +TY RCL+LASA+T LGIS G VVA LAPNV
Sbjct: 62 SPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRGDVVAALAPNV 121
Query: 123 PAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQ 182
PAM+ELHFAVPMAG +LC LN R D S +SVLL HSEAKI+FVD+QL E+A A+ LL +
Sbjct: 122 PAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLAK 181
Query: 183 GDSEPPKLVLIL----------DSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEW 242
D L L+L D + S+ AS S YEYE+L+ SG EFEI KP+ EW
Sbjct: 182 SDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCEW 241
Query: 243 DPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTW 302
DPISINYTSGTTS PKGVVYSHRGAYLNSLATV L M PVYLWT PMFHCNGWCL W
Sbjct: 242 DPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLVW 301
Query: 303 GVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVL 362
GVAAQGGTN+CLRKVSPK IF+ IA+H VTHM GAPTVLNMIVN V++ + LP++V+++
Sbjct: 302 GVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEIM 361
Query: 363 TGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGV 422
TGG+PP PQ+LAKMEE+GF + HLYGLTETYGPGT+C WKP WD L +ER++++ARQGV
Sbjct: 362 TGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQGV 421
Query: 423 HHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGD 482
H+GLE +DV+DP+TM +VP DG T+GE+MFRGNTVMSGYFK+ +AT ++F G WFHSGD
Sbjct: 422 QHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSGD 481
Query: 483 AAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPC 542
AVK+PD YIE+KDRLKDVIISGGENIS+VEVE VL H AVLEAAVVARPD HWG+TPC
Sbjct: 482 LAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTPC 541
Query: 543 AFVVLKEGCN-VTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMG 587
FV LKEG + + +++I +CRD LPHYMAP++I+F ++PKTSTGKVQK++LR++A MG
Sbjct: 542 GFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEMG 601
BLAST of HG10016308 vs. ExPASy Swiss-Prot
Match:
M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)
HSP 1 Score: 789.3 bits (2037), Expect = 3.1e-227
Identity = 382/555 (68.83%), Postives = 457/555 (82.34%), Query Frame = 0
Query: 38 SWKSMTGLVRCPANDVPLSPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAM 97
SWKSM GLV+C AN VPLSPI+FLER++K YRD TS+VYGS +TW +T++RCLKLASA+
Sbjct: 25 SWKSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASAL 84
Query: 98 -TQLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKII 157
T LGIS G VVAT + N+P +YELHFAVPMAG +LCTLNAR+DS+MVS LL HSEAK+I
Sbjct: 85 TTHLGISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLI 144
Query: 158 FVDYQLFEVACEAIQLLEQGDSEPPKLVLILDSEHGSSPASLSSNVYE-YESLIASGSCE 217
FV+ QL E A A+ LL Q D +PP LVL+ DSE S +S+ Y+ Y L+A+GS +
Sbjct: 145 FVEPQLLETARAALDLLAQKDIKPPTLVLLTDSE------SFTSSSYDHYNHLLANGSDD 204
Query: 218 FEIRKPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPM 277
FEIR+PK+EWDPISINYTSGTT+ PK VVYSHRGAYLNS+ATVLL GMG+ VYLW+ PM
Sbjct: 205 FEIRRPKNEWDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTTSVYLWSVPM 264
Query: 278 FHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDR 337
FHCNGWC WG AAQG TN+C+RKVSPKAIF+ I LH VTH APTVLNMIVNSP +
Sbjct: 265 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 324
Query: 338 RL-LPNKVDVLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPD 397
LP+KV+V+TGG+PPPP+V+A+MEEMGF++ H+YGLTET GP C KP WD L P+
Sbjct: 325 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPE 384
Query: 398 ERSRIQARQGVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEE 457
ER ++ARQG++H+ +EE+DVRDPVTM SV ADG TIGE+MFRGNTVMSGYFK+ KATEE
Sbjct: 385 ERYALKARQGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEE 444
Query: 458 SFRGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVA 517
+F GGWF SGD VKH D YI++KDR KDV+ISGGENISTVEVETVL+ H AVLEAAVVA
Sbjct: 445 AFEGGWFRSGDLGVKHEDGYIQLKDRKKDVVISGGENISTVEVETVLYSHEAVLEAAVVA 504
Query: 518 RPDDHWGETPCAFVVLKEGC--NVTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQ 577
RPD WGETPCAFV LKEG +V+A Q+I +CRDRLPHYMAP++++F+ELPKTSTGK+Q
Sbjct: 505 RPDKLWGETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQ 564
Query: 578 KFILRERAKAMGSLS 588
K+IL+E+A AMGSLS
Sbjct: 565 KYILKEKAMAMGSLS 573
BLAST of HG10016308 vs. ExPASy Swiss-Prot
Match:
M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)
HSP 1 Score: 786.2 bits (2029), Expect = 2.6e-226
Identity = 379/555 (68.29%), Postives = 458/555 (82.52%), Query Frame = 0
Query: 38 SWKSMTGLVRCPANDVPLSPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAM 97
SWKSM GLV+C AN VPLSPI+FLER++K YRD TS+VYGS +TW +T++RCLKLASA+
Sbjct: 25 SWKSMEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASAL 84
Query: 98 -TQLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKII 157
T GIS G VVAT + N+P +YELHFAVPMAG +LCTLNAR+DS+MVS LL HSEAK+I
Sbjct: 85 TTHFGISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLI 144
Query: 158 FVDYQLFEVACEAIQLLEQGDSEPPKLVLILDSEHGSSPASLSSNVYE-YESLIASGSCE 217
FV+ QL E A A+ LL Q D +PP LVL+ DSE S +S+ Y+ Y L+A+GS +
Sbjct: 145 FVEPQLLETARAALDLLAQKDIKPPTLVLLTDSE------SFTSSSYDHYNHLLANGSDD 204
Query: 218 FEIRKPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPM 277
FEIR+PK+E DPISINYTSGTT+ PK VVYSHRGAYLNS+ATVLL GMG+ VYLW+ PM
Sbjct: 205 FEIRRPKNECDPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTRSVYLWSVPM 264
Query: 278 FHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDR 337
FHCNGWC WG AAQG TN+C+RKVSPKAIF+ I LH VTH APTVLNMIVNSP +
Sbjct: 265 FHCNGWCFPWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNL 324
Query: 338 RL-LPNKVDVLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPD 397
LP+KV+V+TGG+PPPP+V+A+MEEMGF++ H+YGLTET+GP T C KP WD L P+
Sbjct: 325 HTPLPHKVEVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPE 384
Query: 398 ERSRIQARQGVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEE 457
ER ++ARQG++H+ +EE+DVRDPV+M SV ADG TIGE+MFRGNTVMSGYFK+ KATEE
Sbjct: 385 ERYALKARQGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEE 444
Query: 458 SFRGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVA 517
+F GGWF +GD VKH D YI++KDR KDV+ISGGEN+STVEVETVL+ H AVLEAAVVA
Sbjct: 445 AFEGGWFRTGDLGVKHEDGYIQLKDRKKDVVISGGENVSTVEVETVLYSHEAVLEAAVVA 504
Query: 518 RPDDHWGETPCAFVVLKEGCN--VTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQ 577
RPD WGETPCAFV LKEG + V+A Q+I +CRDRLPHYMAP++++F+ELPKTSTGK+Q
Sbjct: 505 RPDKLWGETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQ 564
Query: 578 KFILRERAKAMGSLS 588
K+IL+E+AKAMGSLS
Sbjct: 565 KYILKEKAKAMGSLS 573
BLAST of HG10016308 vs. ExPASy Swiss-Prot
Match:
F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)
HSP 1 Score: 756.1 bits (1951), Expect = 2.9e-217
Identity = 354/547 (64.72%), Postives = 439/547 (80.26%), Query Frame = 0
Query: 42 MTGLVRCPANDVPLSPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLG 101
M G ++ PAN VPL+PISFL+R+A VY D SIVYGS +TW +T +RC+++ASA++QLG
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62
Query: 102 ISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQ 161
IS+G VV+ LAPNVPAM ELHF VPMAGA+LCTLN RHDSS+V+VLLRHS K+IF D+Q
Sbjct: 63 ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122
Query: 162 LFEVACEAIQLLEQGDSEPPKLVLILDS-EHGSSPASLSSNVYEYESLIASGSCEFEIRK 221
++A A ++L + P LVLI + S S + EYE ++A G +FE+ +
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182
Query: 222 PKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNG 281
P E D IS+NYTSGTTS PKGVVYSHRGAYLNSLA VLL M S P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242
Query: 282 WCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPN 341
WCL WGV A GGTN+CLR V+ KAIF+ I+ H VTHM GAPT+LNMI+N+P S+++ LP
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302
Query: 342 KVDVLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQ 401
KV +TG APPP V+ KMEE+GF + H YGLTETYGPGT CTWKP WD LP +E+++++
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362
Query: 402 ARQGVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGW 461
ARQGV+H+GLEE+ V+DPVTM ++PADG T+GE++FRGNTVM+GY KN +AT+E+F+GGW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422
Query: 462 FHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHW 521
F SGD VKHPD YIE+KDR KD+IISGGENIS++EVE+ LF HP VLEAAVVARPD++W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482
Query: 522 GETPCAFVVLKEGCNVTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERA 581
GET CAFV LK+G +A++LI+YCRDRLPHYMAPRSI+F++LPKTSTGKVQKF+LR +A
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKA 542
Query: 582 KAMGSLS 588
KA+ SLS
Sbjct: 543 KALVSLS 549
BLAST of HG10016308 vs. ExPASy Swiss-Prot
Match:
Q8VZF1 (Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE7 PE=1 SV=1)
HSP 1 Score: 609.8 bits (1571), Expect = 3.3e-173
Identity = 291/557 (52.24%), Postives = 385/557 (69.12%), Query Frame = 0
Query: 39 WKSMTGLVRCPANDVPLSPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMT 98
W+ + L + PAN L+P+ FL+R A V+ S+++GS +TW +TY+RC +LASA+
Sbjct: 6 WRDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALA 65
Query: 99 QLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFV 158
I G VA +APN+PAMYE HF VPM GAVL +N R ++ V+ LL HS++ +I V
Sbjct: 66 DRSIGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMV 125
Query: 159 DYQLFEVACEAIQLLEQ-GDSEPPKLVLILDSEHGSSPAS----LSSNVYEYESLIASGS 218
D + F +A ++++L+E+ S + +LI+ +H +P S LS EYE +A+G
Sbjct: 126 DQEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGD 185
Query: 219 CEFEIRKPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTC 278
+ + P EW I++ YTSGTT+ PKGVV HRGAY+ +L+ L+ GM VYLWT
Sbjct: 186 PNYPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTL 245
Query: 279 PMFHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVS 338
PMFHCNGWC W +A GT++CLR+V+ K ++ IA + VTH AP VLN IVN+P
Sbjct: 246 PMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKE 305
Query: 339 DRRL-LPNKVDVLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLP 398
D L LP+ V V+T GA PPP VL M + GFR+ H YGL+ETYGP T C WKP WD LP
Sbjct: 306 DTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLP 365
Query: 399 PDERSRIQARQGVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKAT 458
P+ ++++ ARQGV + G+E++DV D T VPADGKT GEI+FRGN VM GY KN +A
Sbjct: 366 PETQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEAN 425
Query: 459 EESFRGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAV 518
+E+F GGWFHSGD AVKHPD+YIE+KDR KDVIISGGENIS+VEVE V++ HPAVLEA+V
Sbjct: 426 KETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASV 485
Query: 519 VARPDDHWGETPCAFVVLKEGC-----NVTAQQLINYCRDRLPHYMAPRSIIFQELPKTS 578
VARPD+ W E+PCAFV LK N AQ ++ +CR++LP Y P+S++F LPKT+
Sbjct: 486 VARPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTA 545
Query: 579 TGKVQKFILRERAKAMG 585
TGK+QK ILR +AK MG
Sbjct: 546 TGKIQKHILRTKAKEMG 562
BLAST of HG10016308 vs. ExPASy TrEMBL
Match:
A0A0A0KGV7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G509050 PE=4 SV=1)
HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 552/588 (93.88%), Postives = 574/588 (97.62%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSV+SLNRALWTW+FPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVVSLNRALWTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAK YRDTTSIVYGS SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKAYRDTTSIVYGSMSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPMAGAVLCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAI+LLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIRLLEQGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPAS+SSNVYEYESLIASGSCEFE+R+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASISSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
SPKAIFEKIALHN THMA APTVLNMI NSPVSDRRLLPNKVDVLTGG+PPPPQVLA+ME
Sbjct: 301 SPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLLPNKVDVLTGGSPPPPQVLAEME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWD LP DE+SRI+ARQG+HHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGIHHVGLQEVDVRDPKT 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVP DGKT+GEIMFRGNTVMSGYFKNQKATEE+F GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFMGGWFHSGDAAVKHPDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540
LKDVIISGGENISTVEVET+LF HP+VLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI+YCRDRLPHYMAPR+I+FQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588
BLAST of HG10016308 vs. ExPASy TrEMBL
Match:
A0A1S3B1R0 (probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo OX=3656 GN=LOC103485184 PE=4 SV=1)
HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 555/588 (94.39%), Postives = 573/588 (97.45%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSVLSLNRAL TW+FPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAK YRDTTSIVYGS SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPMAGAVLCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAIQLLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFE+R+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
SPKAIFE IALHN THMA APTVLNMIVNSPVSDRRLLPNKVDVLTGG+PPPPQV A+ME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWD LP DE+SRI+ARQGVHHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVP DGKT+GEIMFRGNTVMSGYFKNQKATEE+F+GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540
LKDVIISGGENISTVEVETVLF HP+VLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI+YCRDRLPHYMAPRSI+FQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588
BLAST of HG10016308 vs. ExPASy TrEMBL
Match:
A0A5A7SME5 (Putative acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00680 PE=4 SV=1)
HSP 1 Score: 1147.5 bits (2967), Expect = 0.0e+00
Identity = 554/588 (94.22%), Postives = 572/588 (97.28%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
MNRFL HLFRSVLSLNRAL TW+FPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF
Sbjct: 1 MNRFLHNHLFRSVLSLNRALSTWNFPSNRRGFSDLPESWKSMTGLVRCPANDVPLSPISF 60
Query: 61 LERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
LERTAK YRDTTSIVYGS SFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE
Sbjct: 61 LERTAKAYRDTTSIVYGSLSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMYE 120
Query: 121 LHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSEP 180
LHFAVPMAGAVLCTLN+RHDSSMVSVLLRHSEAKIIFVD+QLFEVACEAIQLLEQGDSEP
Sbjct: 121 LHFAVPMAGAVLCTLNSRHDSSMVSVLLRHSEAKIIFVDHQLFEVACEAIQLLEQGDSEP 180
Query: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSMP 240
PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFE+R+PKSEWDPISINYTSGTTSMP
Sbjct: 181 PKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEVRRPKSEWDPISINYTSGTTSMP 240
Query: 241 KGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRKV 300
KGVVYSHRGAYLNSLATVLLGGM SMPVYLWTCPMFHCNGWCLTWG+AAQGGTNLCLRKV
Sbjct: 241 KGVVYSHRGAYLNSLATVLLGGMRSMPVYLWTCPMFHCNGWCLTWGIAAQGGTNLCLRKV 300
Query: 301 SPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKME 360
SPKAIFE IALHN THMA APTVLNMIVNSPVSDRRLLPNKVDVLTGG+PPPPQV A+ME
Sbjct: 301 SPKAIFETIALHNATHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGSPPPPQVFAEME 360
Query: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPVT 420
EMGFRICHLYGLTETYGPGTYCTWKPMWD LP DE+SRI+ARQGVHHVGL+EVDVRDP T
Sbjct: 361 EMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSRIRARQGVHHVGLQEVDVRDPET 420
Query: 421 MASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKDR 480
MASVP DGKT+GEIMFRGNTVMSGYFKNQKATEE+F+GGWFHSGDAAVKHPDHYIEVKDR
Sbjct: 421 MASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKGGWFHSGDAAVKHPDHYIEVKDR 480
Query: 481 LKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN-VTAQ 540
LKDVIISGGENISTVEVETVLF HP+VLEAAVVARPDDHWGETPCAFVVLKEGCN VTAQ
Sbjct: 481 LKDVIISGGENISTVEVETVLFSHPSVLEAAVVARPDDHWGETPCAFVVLKEGCNTVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI+YCRDRLPHYMAPRSI+FQ+LPKTSTGKVQKFILRERAKAMGSLS
Sbjct: 541 QLIDYCRDRLPHYMAPRSIVFQDLPKTSTGKVQKFILRERAKAMGSLS 588
BLAST of HG10016308 vs. ExPASy TrEMBL
Match:
A0A6J1BTA3 (probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005564 PE=4 SV=1)
HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 533/592 (90.03%), Postives = 563/592 (95.10%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALW--TW--DFPSNRRGFSDLPESWKSMTGLVRCPANDVPLS 60
MNRFLQ L RSV S NR LW W DFPS+RRGF+++P+SWKSM GLVRCPANDVPLS
Sbjct: 1 MNRFLQNQLLRSVCSFNRPLWNRNWNLDFPSSRRGFANVPDSWKSMAGLVRCPANDVPLS 60
Query: 61 PISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVP 120
P+SFLERTAKVYRDTTS+VYGS SFTWEETYNRCLKLASA++QLGIS GQVVATLAPNVP
Sbjct: 61 PLSFLERTAKVYRDTTSVVYGSVSFTWEETYNRCLKLASAISQLGISPGQVVATLAPNVP 120
Query: 121 AMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQG 180
AMYELHFAVPMAGAVLCTLNARHDSSMVS LLRHSEAKIIFVD+QLFEVACEAIQLL QG
Sbjct: 121 AMYELHFAVPMAGAVLCTLNARHDSSMVSALLRHSEAKIIFVDFQLFEVACEAIQLLAQG 180
Query: 181 DSEPPKLVLILDSEHGSSPAS-LSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSG 240
DSE PKLVLILD+EHG SPA+ LSSNV EYESLIASGSCEFE++KPKSEWDPISINYTSG
Sbjct: 181 DSESPKLVLILDAEHGLSPANPLSSNVCEYESLIASGSCEFEVQKPKSEWDPISINYTSG 240
Query: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNL 300
TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCL WGVAAQGGTN+
Sbjct: 241 TTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLAWGVAAQGGTNI 300
Query: 301 CLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQV 360
CLRKVSPKAIFEKIAL NVTHMA APTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQ+
Sbjct: 301 CLRKVSPKAIFEKIALQNVTHMAAAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQI 360
Query: 361 LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDV 420
LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWD LP DER+RI+ARQGVHHVGL+EVDV
Sbjct: 361 LAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDSLPSDERARIRARQGVHHVGLQEVDV 420
Query: 421 RDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYI 480
RDPVTMA VPADGKT+GEIMFRGNTVMSGYFKNQKATEE+FRGGWFHSGDAAVKHPDHYI
Sbjct: 421 RDPVTMAGVPADGKTMGEIMFRGNTVMSGYFKNQKATEEAFRGGWFHSGDAAVKHPDHYI 480
Query: 481 EVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCN 540
EVKDRLKD++ISGGENISTVEVET+LFGHPAVLEAAVV RPD+HWGETPCAFV LKEGCN
Sbjct: 481 EVKDRLKDIVISGGENISTVEVETILFGHPAVLEAAVVGRPDNHWGETPCAFVTLKEGCN 540
Query: 541 VTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
+TAQQLI+YCRD LPHYMAPRSIIFQ+LPKTSTGK+QKFILR+RAKAMGSLS
Sbjct: 541 ITAQQLIDYCRDHLPHYMAPRSIIFQDLPKTSTGKIQKFILRDRAKAMGSLS 592
BLAST of HG10016308 vs. ExPASy TrEMBL
Match:
A0A6J1KUK0 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111497331 PE=4 SV=1)
HSP 1 Score: 1056.2 bits (2730), Expect = 5.0e-305
Identity = 505/588 (85.88%), Postives = 538/588 (91.50%), Query Frame = 0
Query: 1 MNRFLQTHLFRSVLSLNRALWTWDFPSN-RRGFSDLPESWKSMTGLVRCPANDVPLSPIS 60
MNRF++ FRSV SLNRA+W WDFPS RGFSDL +SWKS GLVRCPANDVPLSP++
Sbjct: 1 MNRFIRNRSFRSVFSLNRAVWKWDFPSTICRGFSDLSDSWKSTAGLVRCPANDVPLSPVT 60
Query: 61 FLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNVPAMY 120
FLERTAKVYRDT S+VYGS SFTWEETYNRCL+LASAMTQLGIS GQVVATLAPN+PAMY
Sbjct: 61 FLERTAKVYRDTASVVYGSVSFTWEETYNRCLRLASAMTQLGISPGQVVATLAPNIPAMY 120
Query: 121 ELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGDSE 180
ELHFA PMAGAVLCTLN RHDSSM+SVLLRHSEAKIIFVD QLFEVA EAIQLL QGDS+
Sbjct: 121 ELHFATPMAGAVLCTLNTRHDSSMLSVLLRHSEAKIIFVDTQLFEVANEAIQLLRQGDSQ 180
Query: 181 PPKLVLILDSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGTTSM 240
PPKLVLI DSE+ SSP SLSSN YEYE+LIASGSCEFE+R P+SEWDPISINYTSGTTSM
Sbjct: 181 PPKLVLIHDSEYASSPTSLSSNAYEYETLIASGSCEFEVRNPRSEWDPISINYTSGTTSM 240
Query: 241 PKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLCLRK 300
PKGVVYSHRGAYLNSLATVLL GMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTN+CLRK
Sbjct: 241 PKGVVYSHRGAYLNSLATVLLAGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNICLRK 300
Query: 301 VSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVLAKM 360
VSPKAIFE+IALH VTHMA APTVLNMIVNSP DRR LPN V VLTGG+PPPP V K+
Sbjct: 301 VSPKAIFEQIALHKVTHMAAAPTVLNMIVNSPECDRRPLPNTVVVLTGGSPPPPHVFTKV 360
Query: 361 EEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVRDPV 420
EEMGF+ICHLYGLTETYGPGTYCTWKPMWD LP DERSRI+ RQGV HVGL +V+V DP
Sbjct: 361 EEMGFQICHLYGLTETYGPGTYCTWKPMWDCLPCDERSRIRVRQGVRHVGLHDVNVIDPK 420
Query: 421 TMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIEVKD 480
TM VPADGKT+GEIMFRGNTVMSGYFKN+KATEE+F+GGWFHSGDA VKHP+HYIEVKD
Sbjct: 421 TMVGVPADGKTVGEIMFRGNTVMSGYFKNKKATEEAFKGGWFHSGDAGVKHPNHYIEVKD 480
Query: 481 RLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCNVTAQ 540
RLKDVIISGGENIS+VEVE VLFGHPA+LEAAVV RPDDHWGETPCAFV LKEGCNVTAQ
Sbjct: 481 RLKDVIISGGENISSVEVEAVLFGHPAILEAAVVGRPDDHWGETPCAFVALKEGCNVTAQ 540
Query: 541 QLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
QLI YCR RLPHYMAPRSI+FQ+LPKTSTGKVQKFILR+RAKAMGSLS
Sbjct: 541 QLIEYCRHRLPHYMAPRSIVFQDLPKTSTGKVQKFILRDRAKAMGSLS 588
BLAST of HG10016308 vs. TAIR 10
Match:
AT2G17650.1 (AMP-dependent synthetase and ligase family protein )
HSP 1 Score: 796.2 bits (2055), Expect = 1.8e-230
Identity = 392/602 (65.12%), Postives = 469/602 (77.91%), Query Frame = 0
Query: 3 RFLQT-HLFRSVLSLNRALWTWDFP----SNRRGFSD--LPESWKSMTGLVRCPANDVPL 62
RFL T FR + LW P SN GF D PESW+++ GL+R PAN PL
Sbjct: 2 RFLLTKRAFRIFNPRFQRLWLTSSPFSSTSNSGGFPDDSEPESWRTIEGLLRSPANFSPL 61
Query: 63 SPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLGISSGQVVATLAPNV 122
SPI+FLER+AKVYRD TS+V+GS TW +TY RCL+LASA+T LGIS G VVA LAPNV
Sbjct: 62 SPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALTNLGISRGDVVAALAPNV 121
Query: 123 PAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQ 182
PAM+ELHFAVPMAG +LC LN R D S +SVLL HSEAKI+FVD+QL E+A A+ LL +
Sbjct: 122 PAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIAHGALDLLAK 181
Query: 183 GDSEPPKLVLIL----------DSEHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEW 242
D L L+L D + S+ AS S YEYE+L+ SG EFEI KP+ EW
Sbjct: 182 SDKTRKSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGDSEFEIIKPRCEW 241
Query: 243 DPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTW 302
DPISINYTSGTTS PKGVVYSHRGAYLNSLATV L M PVYLWT PMFHCNGWCL W
Sbjct: 242 DPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQMSVYPVYLWTVPMFHCNGWCLVW 301
Query: 303 GVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVL 362
GVAAQGGTN+CLRKVSPK IF+ IA+H VTHM GAPTVLNMIVN V++ + LP++V+++
Sbjct: 302 GVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIVNYTVTEHKPLPHRVEIM 361
Query: 363 TGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGV 422
TGG+PP PQ+LAKMEE+GF + HLYGLTETYGPGT+C WKP WD L +ER++++ARQGV
Sbjct: 362 TGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPEWDSLSLEERTKLKARQGV 421
Query: 423 HHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGD 482
H+GLE +DV+DP+TM +VP DG T+GE+MFRGNTVMSGYFK+ +AT ++F G WFHSGD
Sbjct: 422 QHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFKDIEATRKAFEGDWFHSGD 481
Query: 483 AAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPC 542
AVK+PD YIE+KDRLKDVIISGGENIS+VEVE VL H AVLEAAVVARPD HWG+TPC
Sbjct: 482 LAVKYPDGYIEIKDRLKDVIISGGENISSVEVERVLCSHQAVLEAAVVARPDHHWGQTPC 541
Query: 543 AFVVLKEGCN-VTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMG 587
FV LKEG + + +++I +CRD LPHYMAP++I+F ++PKTSTGKVQK++LR++A MG
Sbjct: 542 GFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPKTSTGKVQKYLLRKKADEMG 601
BLAST of HG10016308 vs. TAIR 10
Match:
AT1G20560.1 (acyl activating enzyme 1 )
HSP 1 Score: 756.1 bits (1951), Expect = 2.0e-218
Identity = 354/547 (64.72%), Postives = 439/547 (80.26%), Query Frame = 0
Query: 42 MTGLVRCPANDVPLSPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMTQLG 101
M G ++ PAN VPL+PISFL+R+A VY D SIVYGS +TW +T +RC+++ASA++QLG
Sbjct: 3 MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62
Query: 102 ISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQ 161
IS+G VV+ LAPNVPAM ELHF VPMAGA+LCTLN RHDSS+V+VLLRHS K+IF D+Q
Sbjct: 63 ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122
Query: 162 LFEVACEAIQLLEQGDSEPPKLVLILDS-EHGSSPASLSSNVYEYESLIASGSCEFEIRK 221
++A A ++L + P LVLI + S S + EYE ++A G +FE+ +
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182
Query: 222 PKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNG 281
P E D IS+NYTSGTTS PKGVVYSHRGAYLNSLA VLL M S P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242
Query: 282 WCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPN 341
WCL WGV A GGTN+CLR V+ KAIF+ I+ H VTHM GAPT+LNMI+N+P S+++ LP
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302
Query: 342 KVDVLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQ 401
KV +TG APPP V+ KMEE+GF + H YGLTETYGPGT CTWKP WD LP +E+++++
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362
Query: 402 ARQGVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGW 461
ARQGV+H+GLEE+ V+DPVTM ++PADG T+GE++FRGNTVM+GY KN +AT+E+F+GGW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422
Query: 462 FHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHW 521
F SGD VKHPD YIE+KDR KD+IISGGENIS++EVE+ LF HP VLEAAVVARPD++W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482
Query: 522 GETPCAFVVLKEGCNVTAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERA 581
GET CAFV LK+G +A++LI+YCRDRLPHYMAPRSI+F++LPKTSTGKVQKF+LR +A
Sbjct: 483 GETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKA 542
Query: 582 KAMGSLS 588
KA+ SLS
Sbjct: 543 KALVSLS 549
BLAST of HG10016308 vs. TAIR 10
Match:
AT1G20560.2 (acyl activating enzyme 1 )
HSP 1 Score: 657.5 bits (1695), Expect = 9.9e-189
Identity = 307/471 (65.18%), Postives = 377/471 (80.04%), Query Frame = 0
Query: 118 MYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVACEAIQLLEQGD 177
M ELHF VPMAGA+LCTLN RHDSS+V+VLLRHS K+IF D+Q ++A A ++L
Sbjct: 1 MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60
Query: 178 SEPPKLVLILDS-EHGSSPASLSSNVYEYESLIASGSCEFEIRKPKSEWDPISINYTSGT 237
+ P LVLI + S S + EYE ++A G +FE+ +P E D IS+NYTSGT
Sbjct: 61 DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGT 120
Query: 238 TSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCLTWGVAAQGGTNLC 297
TS PKGVVYSHRGAYLNSLA VLL M S P YLWT PMFHCNGWCL WGV A GGTN+C
Sbjct: 121 TSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGGTNIC 180
Query: 298 LRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVDVLTGGAPPPPQVL 357
LR V+ KAIF+ I+ H VTHM GAPT+LNMI+N+P S+++ LP KV +TG APPP V+
Sbjct: 181 LRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAHVI 240
Query: 358 AKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQGVHHVGLEEVDVR 417
KMEE+GF + H YGLTETYGPGT CTWKP WD LP +E+++++ARQGV+H+GLEE+ V+
Sbjct: 241 FKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQVK 300
Query: 418 DPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHSGDAAVKHPDHYIE 477
DPVTM ++PADG T+GE++FRGNTVM+GY KN +AT+E+F+GGWF SGD VKHPD YIE
Sbjct: 301 DPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGYIE 360
Query: 478 VKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGETPCAFVVLKEGCNV 537
+KDR KD+IISGGENIS++EVE+ LF HP VLEAAVVARPD++WGET CAFV LK+G
Sbjct: 361 LKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDGSKA 420
Query: 538 TAQQLINYCRDRLPHYMAPRSIIFQELPKTSTGKVQKFILRERAKAMGSLS 588
+A++LI+YCRDRLPHYMAPRSI+F++LPKTSTGKVQKF+LR +AKA+ SLS
Sbjct: 421 SAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKALVSLS 471
BLAST of HG10016308 vs. TAIR 10
Match:
AT3G16910.1 (acyl-activating enzyme 7 )
HSP 1 Score: 609.8 bits (1571), Expect = 2.4e-174
Identity = 291/557 (52.24%), Postives = 385/557 (69.12%), Query Frame = 0
Query: 39 WKSMTGLVRCPANDVPLSPISFLERTAKVYRDTTSIVYGSTSFTWEETYNRCLKLASAMT 98
W+ + L + PAN L+P+ FL+R A V+ S+++GS +TW +TY+RC +LASA+
Sbjct: 6 WRDIDDLPKIPANYTALTPLWFLDRAAVVHPTRKSVIHGSREYTWRQTYDRCRRLASALA 65
Query: 99 QLGISSGQVVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFV 158
I G VA +APN+PAMYE HF VPM GAVL +N R ++ V+ LL HS++ +I V
Sbjct: 66 DRSIGPGSTVAIIAPNIPAMYEAHFGVPMCGAVLNCVNIRLNAPTVAFLLSHSQSSVIMV 125
Query: 159 DYQLFEVACEAIQLLEQ-GDSEPPKLVLILDSEHGSSPAS----LSSNVYEYESLIASGS 218
D + F +A ++++L+E+ S + +LI+ +H +P S LS EYE +A+G
Sbjct: 126 DQEFFTLAEDSLRLMEEKAGSSFKRPLLIVIGDHTCAPESLNRALSKGAIEYEDFLATGD 185
Query: 219 CEFEIRKPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTC 278
+ + P EW I++ YTSGTT+ PKGVV HRGAY+ +L+ L+ GM VYLWT
Sbjct: 186 PNYPWQPPADEWQSIALGYTSGTTASPKGVVLHHRGAYIMALSNPLIWGMQDGAVYLWTL 245
Query: 279 PMFHCNGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVS 338
PMFHCNGWC W +A GT++CLR+V+ K ++ IA + VTH AP VLN IVN+P
Sbjct: 246 PMFHCNGWCFPWSLAVLSGTSICLRQVTAKEVYSMIAKYKVTHFCAAPVVLNAIVNAPKE 305
Query: 339 DRRL-LPNKVDVLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLP 398
D L LP+ V V+T GA PPP VL M + GFR+ H YGL+ETYGP T C WKP WD LP
Sbjct: 306 DTILPLPHTVHVMTAGAAPPPSVLFSMNQKGFRVAHTYGLSETYGPSTVCAWKPEWDSLP 365
Query: 399 PDERSRIQARQGVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKAT 458
P+ ++++ ARQGV + G+E++DV D T VPADGKT GEI+FRGN VM GY KN +A
Sbjct: 366 PETQAKLNARQGVRYTGMEQLDVIDTQTGKPVPADGKTAGEIVFRGNMVMKGYLKNPEAN 425
Query: 459 EESFRGGWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAV 518
+E+F GGWFHSGD AVKHPD+YIE+KDR KDVIISGGENIS+VEVE V++ HPAVLEA+V
Sbjct: 426 KETFAGGWFHSGDIAVKHPDNYIEIKDRSKDVIISGGENISSVEVENVVYHHPAVLEASV 485
Query: 519 VARPDDHWGETPCAFVVLKEGC-----NVTAQQLINYCRDRLPHYMAPRSIIFQELPKTS 578
VARPD+ W E+PCAFV LK N AQ ++ +CR++LP Y P+S++F LPKT+
Sbjct: 486 VARPDERWQESPCAFVTLKSDYEKHDQNKLAQDIMKFCREKLPAYWVPKSVVFGPLPKTA 545
Query: 579 TGKVQKFILRERAKAMG 585
TGK+QK ILR +AK MG
Sbjct: 546 TGKIQKHILRTKAKEMG 562
BLAST of HG10016308 vs. TAIR 10
Match:
AT5G16370.1 (acyl activating enzyme 5 )
HSP 1 Score: 574.7 bits (1480), Expect = 8.4e-164
Identity = 287/545 (52.66%), Postives = 376/545 (68.99%), Query Frame = 0
Query: 48 CPANDVPLSPISFLERTAKVYRDTTSIVYGS-TSFTWEETYNRCLKLASAMTQLGISSGQ 107
C AN PL+PI FLER A VY D TSIVYGS T +TW ET RCL++AS+++ +GI
Sbjct: 7 CAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSIGIGRSD 66
Query: 108 VVATLAPNVPAMYELHFAVPMAGAVLCTLNARHDSSMVSVLLRHSEAKIIFVDYQLFEVA 167
VV+ L+PN PAMYEL FAVPM+GA+L +N R D+ VSVLLRH +K++FVD ++A
Sbjct: 67 VVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDVFSVDLA 126
Query: 168 CEAIQLLEQGDSEPPKLVLILDSEHGSSPASLSSNV---YEYESLIASGSCEFEIRKPKS 227
EAI ++ ++PP LV I D E A ++ Y Y+ LI G +F+ +P+S
Sbjct: 127 VEAISMM---TTDPPILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFKWIRPES 186
Query: 228 EWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHCNGWCL 287
EWDP+ +NYTSGTTS PKGVV+ HRG ++ S+ +++ + PVYLWT P+FH NGW
Sbjct: 187 EWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFHANGWSY 246
Query: 288 TWGVAAQGGTNLCLRKVSPKAIFEKIALHNVTHMAGAPTVLNMIVNSPVSDRRLLPNKVD 347
WG+AA GGTN+CLRK I+ I H VTHM GAP VLNM+ S ++ + L V+
Sbjct: 247 PWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNML--SATNEFQPLNRPVN 306
Query: 348 VLTGGAPPPPQVLAKMEEMGFRICHLYGLTETYGPGTYCTWKPMWDGLPPDERSRIQARQ 407
+LT GAPPP VL + E +GF I H YGLTET G C WKP W+ LP +R+R++ARQ
Sbjct: 307 ILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRARLKARQ 366
Query: 408 GVHHVGLEEVDVRDPVTMASVPADGKTIGEIMFRGNTVMSGYFKNQKATEESFRGGWFHS 467
GV VG E+DV DP + SV +G+T+GEI+ RG+++M GY K+ TE++ + GWF++
Sbjct: 367 GVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALKNGWFYT 426
Query: 468 GDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETVLFGHPAVLEAAVVARPDDHWGET 527
GD V H D Y+E+KDR KD+II+GGEN+S+VEVETVL+ +PAV E AVVARPD WGET
Sbjct: 427 GDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPDVFWGET 486
Query: 528 PCAFVVLKEGC--NVTAQQLINYCRDRLPHYMAPRSIIF-QELPKTSTGKVQKFILRERA 586
PCAFV LK G T ++I YCR ++P YM P+++ F ELPKTSTGKV KF+LRE A
Sbjct: 487 PCAFVSLKSGLTQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKVMKFVLREIA 546
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038882615.1 | 0.0e+00 | 95.23 | probable acyl-activating enzyme 1, peroxisomal [Benincasa hispida] | [more] |
| XP_004134830.2 | 0.0e+00 | 93.88 | probable acyl-activating enzyme 2 isoform X1 [Cucumis sativus] | [more] |
| XP_008440901.1 | 0.0e+00 | 94.39 | PREDICTED: probable acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis me... | [more] |
| KAA0025615.1 | 0.0e+00 | 94.22 | putative acyl-activating enzyme 1, peroxisomal isoform X1 [Cucumis melo var. mak... | [more] |
| XP_022132791.1 | 0.0e+00 | 90.03 | probable acyl-activating enzyme 1, peroxisomal isoform X1 [Momordica charantia] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9SEY5 | 2.5e-229 | 65.12 | Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1 | [more] |
| M4IRL4 | 3.1e-227 | 68.83 | Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1 | [more] |
| M4IS92 | 2.6e-226 | 68.29 | Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1 | [more] |
| F4HUK6 | 2.9e-217 | 64.72 | Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1 | [more] |
| Q8VZF1 | 3.3e-173 | 52.24 | Acetate/butyrate--CoA ligase AAE7, peroxisomal OS=Arabidopsis thaliana OX=3702 G... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A0A0KGV7 | 0.0e+00 | 93.88 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G509050 PE=4 SV=1 | [more] |
| A0A1S3B1R0 | 0.0e+00 | 94.39 | probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo OX=365... | [more] |
| A0A5A7SME5 | 0.0e+00 | 94.22 | Putative acyl-activating enzyme 1, peroxisomal isoform X1 OS=Cucumis melo var. m... | [more] |
| A0A6J1BTA3 | 0.0e+00 | 90.03 | probable acyl-activating enzyme 1, peroxisomal isoform X1 OS=Momordica charantia... | [more] |
| A0A6J1KUK0 | 5.0e-305 | 85.88 | probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LO... | [more] |
