HG10016295 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10016295 vs. NCBI nr
Match: XP_038883514.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1531.2 bits (3963), Expect = 0.0e+00
Identity = 799/938 (85.18%), Postives = 828/938 (88.27%), Query Frame = 0

Query: 30  FLILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDS 89
           FL  + SSDNS+FSVLNPY DNSEGVARIGGRSLLSLPLK KSSTALIAALDGAIHLVDS
Sbjct: 22  FLGSSKSSDNSEFSVLNPYWDNSEGVARIGGRSLLSLPLKSKSSTALIAALDGAIHLVDS 81

Query: 90  NSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKL 149
           NS+KIIWSFSSG PIYSSYQA INHEHNQENASGVSRSFFFDCGDDWELYIHTE+GKMKL
Sbjct: 82  NSMKIIWSFSSGPPIYSSYQAIINHEHNQENASGVSRSFFFDCGDDWELYIHTEHGKMKL 141

Query: 150 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNY 209
           PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTG+LIRNHMSN LSSEL NEG G Y
Sbjct: 142 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGDLIRNHMSNSLSSELNNEGIGGY 201

Query: 210 KSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPD 269
           KSK NLDIKDLMQS VMN V PRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPD
Sbjct: 202 KSKKNLDIKDLMQSAVMNPVGPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPD 261

Query: 270 VENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRLSEAHNISNM 329
           VENPIEGIPW LQNNNSF VDFAMPLSCQSKALVFRERS FLSGPSGYKRLSEAHNI NM
Sbjct: 262 VENPIEGIPWILQNNNSFGVDFAMPLSCQSKALVFRERSHFLSGPSGYKRLSEAHNIDNM 321

Query: 330 SLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQKM 389
           SLVPASGS LPS+L V +HI+THPE FML GP  NIAS AV  MP    N+SSIIQ+QKM
Sbjct: 322 SLVPASGSLLPSQLNVAEHIDTHPEKFMLHGPV-NIASSAVGSMP---FNESSIIQEQKM 381

Query: 390 GTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKNKPRKS 449
           G   G VGLFFVFLITMLVGLM YGRTLAV+VKQ  LKEKS STSNSRV SSKKNKPRKS
Sbjct: 382 GARPGAVGLFFVFLITMLVGLMMYGRTLAVQVKQFFLKEKSSSTSNSRVISSKKNKPRKS 441

Query: 450 KRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATG 509
           KRS NS KRDVSISSEI D  MQSE +L NGFH+NNLTNIA SGR IGKLF+TNK+IATG
Sbjct: 442 KRSANSGKRDVSISSEIGDMFMQSEDNLNNGFHNNNLTNIASSGRWIGKLFITNKEIATG 501

Query: 510 SNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL 569
           SNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL
Sbjct: 502 SNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL 561

Query: 570 SLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRP 629
           SLERCTCSLDDLIQIYS+L MN M  L+R TG MDEYN+HLESIKD+MPNL LWKE GRP
Sbjct: 562 SLERCTCSLDDLIQIYSNLSMNYMPDLDRATGRMDEYNMHLESIKDSMPNLKLWKETGRP 621

Query: 630 SSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD 689
           +SVLLKLM             RD+VAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD
Sbjct: 622 TSVLLKLM-------------RDVVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD 681

Query: 690 MGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQ 749
           MGISKRLPANVSSLGHRAT                             GCGSSGWQAPEQ
Sbjct: 682 MGISKRLPANVSSLGHRAT-----------------------------GCGSSGWQAPEQ 741

Query: 750 LLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLI 809
           LLRGRQTRAID FSLGCVLFFCITGGRHPFGD LERDVNIVKNKMNLLLV+NIPEAVDLI
Sbjct: 742 LLRGRQTRAIDSFSLGCVLFFCITGGRHPFGDHLERDVNIVKNKMNLLLVDNIPEAVDLI 801

Query: 810 SRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAPLA 869
           S+LLNPNP LRPKASEVL+HPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAP A
Sbjct: 802 SQLLNPNPSLRPKASEVLRHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAPQA 861

Query: 870 LGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGF 929
           LGTEWD+K+E IFI NIGRYRRYKY SVRDLLRVMRNKLNHYRELP+EIQELIGSIPEGF
Sbjct: 862 LGTEWDEKMEPIFIANIGRYRRYKYYSVRDLLRVMRNKLNHYRELPEEIQELIGSIPEGF 913

Query: 930 DDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHVD 968
           DDYFT RFPRLLIEV+KVIS FCRQEECFQKYF+SHVD
Sbjct: 922 DDYFTRRFPRLLIEVYKVISWFCRQEECFQKYFKSHVD 913

BLAST of HG10016295 vs. NCBI nr
Match: XP_008440915.1 (PREDICTED: serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucumis melo])

HSP 1 Score: 1505.7 bits (3897), Expect = 0.0e+00
Identity = 779/940 (82.87%), Postives = 828/940 (88.09%), Query Frame = 0

Query: 30  FLILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDS 89
           FL+ + S +NS+FSVLNPYG NSEGV RIGGRSLLSLPLK KSSTALIAALDG IHLVDS
Sbjct: 19  FLVSSKSFENSEFSVLNPYGVNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGTIHLVDS 78

Query: 90  NSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKL 149
           NS+KIIWSFSSG PIYSSYQANINHEHNQENASGV  SFFFDCGDDWELYIHTE+GKMKL
Sbjct: 79  NSMKIIWSFSSGPPIYSSYQANINHEHNQENASGVGSSFFFDCGDDWELYIHTEHGKMKL 138

Query: 150 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNY 209
           PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVD VTGELIRNHMS  LSSEL NE  G+Y
Sbjct: 139 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGELIRNHMSKFLSSELSNEEPGSY 198

Query: 210 KSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPD 269
           KSK N+DIKDLMQS +MN VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPD
Sbjct: 199 KSKQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPD 258

Query: 270 VENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISN 329
           VENPIEGIPWTLQNNNSF +DFAMPLSCQSKALVFR+RS FLSGPSGYKRL SEAHN +N
Sbjct: 259 VENPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRSHFLSGPSGYKRLPSEAHNSNN 318

Query: 330 MSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQK 389
           +SLV ASGSFLPS+L V KHINT  E FML GP +N  S  VVP+PS+KIN+S+IIQ+QK
Sbjct: 319 ISLVSASGSFLPSQLTVGKHINTGSEKFMLTGPVNN-TSYRVVPLPSMKINESNIIQEQK 378

Query: 390 MGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPR 449
           MGTL G  GLFFVFL+TMLVGLMKYGRTLAV+VKQS LKE+ S  TSNSRV SSKKNKPR
Sbjct: 379 MGTLPGAFGLFFVFLLTMLVGLMKYGRTLAVKVKQSFLKEQSSLGTSNSRVISSKKNKPR 438

Query: 450 KSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIA 509
           KSK+S +S KR+VSISSEIED L+Q E +L NGFHDNNLTNIAGSGRR+GKL+VTNK+IA
Sbjct: 439 KSKKSGSSGKREVSISSEIEDMLLQREDNLNNGFHDNNLTNIAGSGRRVGKLWVTNKEIA 498

Query: 510 TGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFV 569
           TGSNGTIILEGIYEGRPVAVKRLVKTHHD+ SKEVQNLIVSDRHPNIVRWYGMDSDQDFV
Sbjct: 499 TGSNGTIILEGIYEGRPVAVKRLVKTHHDIGSKEVQNLIVSDRHPNIVRWYGMDSDQDFV 558

Query: 570 YLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKG 629
           YLSLERCTCSL DLI+IYS LPMNSMLGL++DTG MDEYN+HLESIK AMPNL LW E G
Sbjct: 559 YLSLERCTCSLYDLIKIYSDLPMNSMLGLDQDTGRMDEYNIHLESIKVAMPNLKLWNENG 618

Query: 630 RPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL 689
           RPSSVLLKLM             RD+VAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL
Sbjct: 619 RPSSVLLKLM-------------RDIVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL 678

Query: 690 SDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAP 749
           SDMGISKRLPANVSSLGHRAT                             GCGSSGWQAP
Sbjct: 679 SDMGISKRLPANVSSLGHRAT-----------------------------GCGSSGWQAP 738

Query: 750 EQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVD 809
           EQLL GRQTRAIDLFSLGCVLFFC+TGGRHPFGD LERDVNIV NKMNLLLV+NIPE VD
Sbjct: 739 EQLLHGRQTRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVD 798

Query: 810 LISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAP 869
           LIS+LLNPNPGLRPKASEVLQHPLFWS EMRLSFLRDTSDRIELEDRET+LLKALESTA 
Sbjct: 799 LISQLLNPNPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRETDLLKALESTAQ 858

Query: 870 LALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPE 929
           +ALG +W++KLE IFI NIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL+GSIP 
Sbjct: 859 IALGLKWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPV 915

Query: 930 GFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHVD 968
           GFDDYFT RFP+LLIEV+KVIS FCRQEECFQKYF+SHVD
Sbjct: 919 GFDDYFTRRFPKLLIEVYKVISCFCRQEECFQKYFKSHVD 915

BLAST of HG10016295 vs. NCBI nr
Match: XP_004134998.1 (serine/threonine-protein kinase/endoribonuclease IRE1a isoform X1 [Cucumis sativus] >KGN48999.1 hypothetical protein Csa_003598 [Cucumis sativus])

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 736/934 (78.80%), Postives = 791/934 (84.69%), Query Frame = 0

Query: 35  LSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDSNSLKI 94
           L S NS+ SVLN YG NSEGV RIGGRSLLSLPLK KSSTALIAALDGAIHLVDSNS+KI
Sbjct: 20  LVSSNSELSVLNLYGGNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGAIHLVDSNSMKI 79

Query: 95  IWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKLPSTID 154
           IWSFSSG PIYSSYQANINHE NQENASGV  SFFFDCGDDWELYIHTE+GKMKLPSTID
Sbjct: 80  IWSFSSGPPIYSSYQANINHEPNQENASGVGSSFFFDCGDDWELYIHTEHGKMKLPSTID 139

Query: 155 EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNYKSKNN 214
           EVVRNTPYIFEDGAVMTGSRKT VFEVD VTGELIRNHMS  LSS L NE   +YKSK+N
Sbjct: 140 EVVRNTPYIFEDGAVMTGSRKTAVFEVDLVTGELIRNHMSKFLSSGLSNEEQVSYKSKHN 199

Query: 215 LDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPDVENPI 274
           +DIKDLMQS  MN+VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPDVENPI
Sbjct: 200 MDIKDLMQS--MNSVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPDVENPI 259

Query: 275 EGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISNMSLVP 334
           EGIPWTLQNNNSF +D+ +PLSCQSKALVFR+RS FLSGPSGYK L SEAH+  NM    
Sbjct: 260 EGIPWTLQNNNSFGIDYGVPLSCQSKALVFRDRSHFLSGPSGYKILSSEAHDSDNM---- 319

Query: 335 ASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQKMGTLA 394
            SGSFLPS+LK+ KHIN     FM  G  +N  S AV P+PS+KIN+S+IIQKQKMG L 
Sbjct: 320 -SGSFLPSQLKIGKHINAKSGKFMFHGLVNN-TSYAVDPLPSMKINESNIIQKQKMGILP 379

Query: 395 GVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPRKSKRS 454
              GLFFVFL+TMLVGLM+YGRTL  +VKQ LLKEK S  TSNSR NSSKKNKPRK K+S
Sbjct: 380 EAFGLFFVFLLTMLVGLMRYGRTLTEKVKQFLLKEKLSLGTSNSRDNSSKKNKPRKLKKS 439

Query: 455 ENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATGSNG 514
             S KR+VSISSEIED L+Q E +L +GFH NNL     SGR IGKL++TNKKIATGSNG
Sbjct: 440 --SGKREVSISSEIEDMLLQRENNLNSGFHGNNLI----SGRHIGKLWITNKKIATGSNG 499

Query: 515 TIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLE 574
           T+ILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGM+SDQDFVYLSLE
Sbjct: 500 TVILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMESDQDFVYLSLE 559

Query: 575 RCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRPSSV 634
           RCTC+L DLIQIYS LP N +LGL+RD+GHMD YN+HLESIK A+PNL LW E GRPSS+
Sbjct: 560 RCTCNLYDLIQIYSDLPKNFVLGLDRDSGHMDGYNIHLESIKVALPNLKLWNENGRPSSI 619

Query: 635 LLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGI 694
           LLKLM             RD+V GL+HLHELGIIHRDLKPQNVLI+KQ+SICSKLSDMGI
Sbjct: 620 LLKLM-------------RDIVVGLKHLHELGIIHRDLKPQNVLILKQKSICSKLSDMGI 679

Query: 695 SKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQLLR 754
           SKRLPANVSSLGH AT                             GCGSSGWQAPEQLL 
Sbjct: 680 SKRLPANVSSLGHHAT-----------------------------GCGSSGWQAPEQLLH 739

Query: 755 GRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLISRL 814
            RQTRA+DLFSLGCVLFFCITGGRHPFGD LERDVNIV NKMNLLLV+NIPE VDLI RL
Sbjct: 740 ERQTRAVDLFSLGCVLFFCITGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVDLICRL 799

Query: 815 LNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAPLALGT 874
           LNPNPGLRPKAS+VLQHPLFWS EMRLSFLRDTSDRIELEDRE+NLLKALESTA +ALG 
Sbjct: 800 LNPNPGLRPKASKVLQHPLFWSPEMRLSFLRDTSDRIELEDRESNLLKALESTAQIALGI 859

Query: 875 EWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGFDDY 934
           +W++KLE IFI NIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL+GSIPEGFDDY
Sbjct: 860 KWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPEGFDDY 897

Query: 935 FTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHV 967
           FTTRFP+LLIEV+KV S FCR EECF+KYF+SHV
Sbjct: 920 FTTRFPKLLIEVYKVTSCFCRHEECFKKYFKSHV 897

BLAST of HG10016295 vs. NCBI nr
Match: XP_022133363.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Momordica charantia] >XP_022133365.1 serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Momordica charantia])

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 705/959 (73.51%), Postives = 800/959 (83.42%), Query Frame = 0

Query: 19  LLTITWRSPVGFLILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIA 78
           L+ + +RS +G    + S +NS+FSVL+PYGDNSEG+AR GGRSLLSLPLK K +TAL+A
Sbjct: 14  LIILRFRSSIG---ESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPLKGKINTALVA 73

Query: 79  ALDGAIHLVDSNSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWEL 138
           A DGAIHLVDSNS+  IWSF+SG PIYSSYQA+I H+HNQENASG+S SFFFDCGDDWEL
Sbjct: 74  ASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSFFFDCGDDWEL 133

Query: 139 YIHTEYGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLS 198
           Y+HTE+GKMKLPS+IDEVVRNTPYIFEDGAVM GSRKTTVFEVDPVTG+LIRNH+S+  S
Sbjct: 134 YMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSS 193

Query: 199 SELRNEGFGNYKSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNV 258
           SEL NE   +Y SK NLD KDL+Q GV++ VEPRLYITRTDYSLKSSFS SEEV+WSLNV
Sbjct: 194 SELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNV 253

Query: 259 AEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYK 318
           AEIGATLLCP+VENP + +PW LQ+N SF +DFAMPLSCQSK +VFRERS   SG SGYK
Sbjct: 254 AEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYK 313

Query: 319 RLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASP--------AV 378
           RLSEAHNI NMSLVPASG  LPS+LKV +  NTHPE  MLPGPA NI+SP        AV
Sbjct: 314 RLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAV 373

Query: 379 VPMPSVKINKSSIIQKQKMGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEKS 438
           +PMP +KIN+SS++Q  KMGTL G  GLFFVFL+T+LVGL+ + R L  +VKQ  L+EK 
Sbjct: 374 IPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKL 433

Query: 439 FSTSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIA 498
            STSNS++ SSKKNK RKSKR+ NS+KRD+S+SSE ED LM SEG++ N FH +NL + A
Sbjct: 434 SSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNA 493

Query: 499 GSGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDR 558
           G+GRRIGKLFVTNK+IA GSNGTI+LEG+YEGRPVAVKRLVKTHHDVA KEVQNLI SDR
Sbjct: 494 GTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDR 553

Query: 559 HPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHL 618
           HPNIVRWYG++SDQDFVYLSLERCTCSLDDLIQI S    NSM GL++DT  M +Y LHL
Sbjct: 554 HPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDTEPMIKYRLHL 613

Query: 619 ESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDL 678
           ES+KD MP+L LWKE G PSSVLLKLM             RDMVAG+EHLHELGIIHRDL
Sbjct: 614 ESVKDVMPDLKLWKENGHPSSVLLKLM-------------RDMVAGVEHLHELGIIHRDL 673

Query: 679 KPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVI 738
           KPQNVLI+KQ+S+C+KLSDMGISKRL A++SSLGH AT                      
Sbjct: 674 KPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT---------------------- 733

Query: 739 RDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIV 798
                  GCGSSGWQAPEQLL GRQTRA+DLFSLGCVLFFCITGGRHPFGDRLERDVNIV
Sbjct: 734 -------GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIV 793

Query: 799 KNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIE 858
           KN+M+L LV +IPEAVDLIS+LLNP+P LRPKASEVLQHPLFWSSE RLSFLRDTSDR+E
Sbjct: 794 KNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVE 853

Query: 859 LEDR--ETNLLKALESTAPLALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKL 918
           LEDR  E+ LLKALE TA LALGT+WD+K+E IFITNIGRYRRYKYDSVRDLLRVMRNKL
Sbjct: 854 LEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKL 913

Query: 919 NHYRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHVD 968
           NHYRELP EIQELIGS+PEGFD YF +RFPRLLIEV+K+IS++CRQEECFQKYF+SH++
Sbjct: 914 NHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 927

BLAST of HG10016295 vs. NCBI nr
Match: TYK12480.1 (serine/threonine-protein kinase/endoribonuclease IRE1a-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1353.6 bits (3502), Expect = 0.0e+00
Identity = 711/879 (80.89%), Postives = 760/879 (86.46%), Query Frame = 0

Query: 32  ILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDSNS 91
           +L L S + +FSVLNPYG NSEGV RIGGRSLLSLPLK KSSTALIAALDG IHLVDSNS
Sbjct: 1   MLILVSYSLEFSVLNPYGVNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGTIHLVDSNS 60

Query: 92  LKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKLPS 151
           +KIIWSFSSG PIYSSYQANINHEHNQENASGV  SFFFDCGDDWELYIHTE+GKMKLPS
Sbjct: 61  MKIIWSFSSGPPIYSSYQANINHEHNQENASGVGSSFFFDCGDDWELYIHTEHGKMKLPS 120

Query: 152 TIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNYKS 211
           TIDEVVRNTPYIFEDGAVMTGSRKTTVFEVD VTGELIRNHMS  LSSEL NE  G+YKS
Sbjct: 121 TIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGELIRNHMSKFLSSELSNEEPGSYKS 180

Query: 212 KNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPDVE 271
           K N+DIKDLMQS +MN VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPDVE
Sbjct: 181 KQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPDVE 240

Query: 272 NPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISNMS 331
           NPIEGIPWTLQNNNSF +DFAMPLSCQSKALVFR+RS FLSGPSGYKRL SEAHN +N+S
Sbjct: 241 NPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRSHFLSGPSGYKRLPSEAHNSNNIS 300

Query: 332 LVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQKMG 391
           LV ASGSFLPS+L V KHINT  E FMLPGP +N  S  VVP+PS+KIN+S+IIQ+QKMG
Sbjct: 301 LVSASGSFLPSQLTVGKHINTGSEKFMLPGPVNN-TSYRVVPLPSMKINESNIIQEQKMG 360

Query: 392 TLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPRKS 451
           TL G  GLFFVFL+TMLVGLMKYGRTLAV+VKQS LKE+ S  TSNSRV SSKKNKPRKS
Sbjct: 361 TLPGAFGLFFVFLLTMLVGLMKYGRTLAVKVKQSFLKEQSSLGTSNSRVISSKKNKPRKS 420

Query: 452 KRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATG 511
           K+S +S KR+VSISSEIED L+Q E +L NGFHDNNLTNIAGSGRR+GKL+VTNK+IATG
Sbjct: 421 KKSGSSGKREVSISSEIEDMLLQREDNLNNGFHDNNLTNIAGSGRRVGKLWVTNKEIATG 480

Query: 512 SNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL 571
           SNGTIILEGIYEGRPVAVKRLVKTHHD+ SKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL
Sbjct: 481 SNGTIILEGIYEGRPVAVKRLVKTHHDIGSKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL 540

Query: 572 SLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRP 631
           SLERCTCSL DLI+IYS LPMNSMLGL++DTG MDEYN+HLESIK AMPNL LW E GRP
Sbjct: 541 SLERCTCSLYDLIKIYSDLPMNSMLGLDQDTGRMDEYNIHLESIKVAMPNLKLWNENGRP 600

Query: 632 SSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD 691
           SSVLLKLM             RD+VAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD
Sbjct: 601 SSVLLKLM-------------RDIVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD 660

Query: 692 MGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQ 751
           MGISKRLPANVSSLGHRAT                             GCGSSGWQAPEQ
Sbjct: 661 MGISKRLPANVSSLGHRAT-----------------------------GCGSSGWQAPEQ 720

Query: 752 LLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLI 811
           LL GRQTRAIDLFSLGCVLFFC+TGGRHPFGD LERDVNIV NKMNLLLV+NIPE VDLI
Sbjct: 721 LLHGRQTRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVDLI 780

Query: 812 SRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAPLA 871
           S+LLNPNPGLRPKASEVLQHPLFWS EMRLSFLRDTSDRIELEDRET+LLKALESTA +A
Sbjct: 781 SQLLNPNPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRETDLLKALESTAQIA 836

Query: 872 LGTEWDKKLERIFITNIGR--YRRYKYDSVRDLLRVMRN 907
           LG +W++KLE IFI NIGR    R+++D +  LL   +N
Sbjct: 841 LGLKWNEKLEPIFIANIGRSLLDRFRWDLMTTLLEGFQN 836

BLAST of HG10016295 vs. ExPASy Swiss-Prot
Match: Q9C5S2 (Serine/threonine-protein kinase/endoribonuclease IRE1a OS=Arabidopsis thaliana OX=3702 GN=IRE1A PE=1 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 1.3e-185
Identity = 417/956 (43.62%), Postives = 554/956 (57.95%), Query Frame = 0

Query: 30  FLILNLSSDNSQFSVLNPYGDNSEGV------ARIGGRSLLSLPLK--DKSSTALIAALD 89
           FL+L LS       +++P G  ++ V      +  G RS+L +  +   + +T L+   D
Sbjct: 13  FLLLLLSP-----WIMSPCGGAADDVTYPIVPSSPGRRSILQIRREPPTEPNTKLVVDRD 72

Query: 90  GAIHLVDSNSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIH 149
           G + L         WSFS+G P++S YQA  N  +N ENA+ ++R            +I 
Sbjct: 73  GKVFLKQQPKETPYWSFSTGSPMHSLYQAPAN--NNTENATEITRP-----------HII 132

Query: 150 TEY-GKMKLPSTID--------EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNH 209
            EY    K  +T+D        E  R  P + +DG V  GS  T+ + VD  +G LI  +
Sbjct: 133 VEYLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDG-VTLGSETTSAYLVDGRSGRLIHVY 192

Query: 210 MSNLLSSELRNEGFGNYKSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEV 269
            S            G+ K  N L +K       +N  EP L I RTD  L+     + ++
Sbjct: 193 KST-----------GDTKITNAL-VKPASTEDFVN--EP-LLIRRTDSKLEHFSKTTGKL 252

Query: 270 SWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLS 329
            W+L V+   A LLC  V N    +   LQ          MPL C S+  V         
Sbjct: 253 VWNLTVSHFRAALLCDPVFNSGYDLGPKLQTG------IYMPLLCGSQIDV--------R 312

Query: 330 GPSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVP 389
           GP    R+                   P  +K            MLP P+ N        
Sbjct: 313 GPEIVIRVLHDQ---------------PMNVK------------MLPSPSLNHFESENSI 372

Query: 390 MPSVKINKSSIIQKQKMGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEKSFS 449
           MP  K  +S  +Q+Q       + G +    + +L  L+  G  ++V +K+   +    S
Sbjct: 373 MPFGKARESRKLQEQHKQKYTYLFGQWSP--VKLLAPLVLLGVVVSVFIKKFSSRGSDVS 432

Query: 450 TSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGS 509
               +   SKK K RKS +  N +    S+    +   +   G +  GF  NN  + A  
Sbjct: 433 L---KAGPSKKKKNRKSAKDTNRQ----SVPRGQDQFELIEGGQMLLGF--NNFQSGATD 492

Query: 510 GRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHP 569
           GR+IGKLF+++K+IA GSNGT++ EGIYEGRPVAVKRLV++HH+VA KE+QNLI SD+H 
Sbjct: 493 GRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRPVAVKRLVRSHHEVAFKEIQNLIASDQHT 552

Query: 570 NIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLES 629
           NI+RWYG++ DQDFVYLSLERCTCSLDDLI+ Y    M  +L     T  +  Y + L+S
Sbjct: 553 NIIRWYGVEYDQDFVYLSLERCTCSLDDLIKSYLEFSMTKVLENNDSTEGVAAYKIQLDS 612

Query: 630 IKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKP 689
           ++  +     WK  G PS ++LKLM             RD+V G+ HLHELGI+HRDLKP
Sbjct: 613 LEGVIKGNNFWKVGGHPSPLMLKLM-------------RDIVCGIVHLHELGIVHRDLKP 672

Query: 690 QNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRD 749
           QNVLI K  ++ +KLSDMGISKR+  ++SSLGH ATG                       
Sbjct: 673 QNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATGS---------------------- 732

Query: 750 KNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKN 809
                  GSSGWQAPEQLL+GRQTRA+D+FSLGCV+F+ ITG +HPFGD LERDVNIVKN
Sbjct: 733 -------GSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKN 792

Query: 810 KMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELE 869
           K++L LV ++PEA DLISRLLNP+P LRP A+EVL HP+FW+SEMRLSFLRD SDR+ELE
Sbjct: 793 KVDLFLVEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELE 840

Query: 870 DRE--TNLLKALESTAPLALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNH 929
           +RE  + +LKA+ESTAP+A+G +WD+KLE +FITNIGRYRRYKYDS+RDLLRV+RNKLNH
Sbjct: 853 NREADSEILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNH 840

Query: 930 YRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHV 967
           +RELP EIQEL+G++PEGFD+YF  RFP+LLIEV++VIS  CR+EE F+KYF+  +
Sbjct: 913 HRELPPEIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFKCDI 840

BLAST of HG10016295 vs. ExPASy Swiss-Prot
Match: Q93VJ2 (Serine/threonine-protein kinase/endoribonuclease IRE1b OS=Arabidopsis thaliana OX=3702 GN=IRE1B PE=2 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 2.5e-178
Identity = 391/917 (42.64%), Postives = 546/917 (59.54%), Query Frame = 0

Query: 71  KSSTALIAALDGAIHLVDSNSLKIIWSFSSGLPIYSSYQA-NINHEHNQENASGVSRSFF 130
           +S   L++ +DG+I LVD +S K+ W+F +  PIYSSYQA + ++  ++E +S +   F+
Sbjct: 45  ESGYVLVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFY 104

Query: 131 FDCGDDWELYIHTEYGKMKLPSTID--EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGE 190
            DC  DW LY  +     ++   +D  E +   PY   D  V+ G + T+VF +D  TG+
Sbjct: 105 MDCDKDWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGK 164

Query: 191 LIRNHMSNLLSSELRNEGFGNYKSKNNLDIKD---LMQSGVMNTVE-PRL-YITRTDYSL 250
           L++ +  + L S   N    N K K  +  K+   L  SG   + + P L YI R D+ +
Sbjct: 165 LVKRYRMDELYS---NTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKI 224

Query: 251 KSSFSNSEEVSWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKAL 310
           +   S   +V WS++ A++ A L   +    I G+  ++  N         PLS  +   
Sbjct: 225 Q-CISKFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVP 284

Query: 311 VFRERSQFLSGPSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPG-- 370
           + + R+        Y+ L       + +L        P++L +            LPG  
Sbjct: 285 MVQLRN------VKYETLFPRLGFLDEALYLPFQDRKPNQLAI-----GDGNQLTLPGNK 344

Query: 371 PASNIASPAVVPMPSVKINK------SSIIQKQKMGTLAGVVGLFFVFLITML--VGLMK 430
            A  + S   +P+P   I++       S  Q       +G++ L F F +TML   GL  
Sbjct: 345 EAEEVLS---LPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFF 404

Query: 431 YGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIED-TLM 490
           Y    ++R+K+  + E   +T   +   SKKN   K+      +K +  IS   +D +  
Sbjct: 405 YRLRQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVH----KKENGFISGGNKDPSHE 464

Query: 491 QSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLV 550
           ++E  L   F    L N +  G R+GKLFV+NK+IA GSNGT++LEG YEGR VAVKRLV
Sbjct: 465 ENEKRLLTAF--PGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRLV 524

Query: 551 KTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMN 610
           ++HHDVA KE+ NL+ SD+H NIVRWYG+D D+ F+Y+SLE C CSL+DLI   S L   
Sbjct: 525 QSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASSAL--- 584

Query: 611 SMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYR 670
               LE        +++ +  I +    + LWKE G PS VLLKLM             R
Sbjct: 585 ----LESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLM-------------R 644

Query: 671 DMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKY 730
           D+VAGL HLH++GI+HRDLKPQNVLIVK  S+C+KLSDMGISKRLPA+ S+L   +TG  
Sbjct: 645 DIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG-- 704

Query: 731 PSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFC 790
                                    +G GSSGWQAPEQL   RQTRA+DLFSLGCVLFFC
Sbjct: 705 -------------------------LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFC 764

Query: 791 ITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPL 850
           +TGG+HP+GD  ERDVN++ ++ +L L+ ++PEAV L++ LLNP+P LRP+A +V+ HPL
Sbjct: 765 MTGGKHPYGDNYERDVNVLNDQKDLFLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPL 824

Query: 851 FWSSEMRLSFLRDTSDRIELEDRE--TNLLKALESTAPLALGTEWDKKLERIFITNIGRY 910
           FW+S+MRLSFLRD SDR+ELE+RE  + LL ALESTA + L   WD+KL+ IF+ NIGRY
Sbjct: 825 FWNSDMRLSFLRDASDRVELENREEGSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRY 880

Query: 911 RRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVIS 967
           RRYK+DS+RDLLRV+RNKLNHYRELPKE+QEL+GS+PEGF+ YF++RFP+LLI+V+ V+ 
Sbjct: 885 RRYKFDSIRDLLRVIRNKLNHYRELPKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLF 880

BLAST of HG10016295 vs. ExPASy Swiss-Prot
Match: Q7XIT1 (Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Oryza sativa subsp. japonica OX=39947 GN=IRE1 PE=1 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 1.5e-138
Identity = 331/892 (37.11%), Postives = 468/892 (52.47%), Query Frame = 0

Query: 81  DGAIHLVDSNSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELY- 140
           +G I LVD  S    W FS+G P+          +H   +   ++   + D  +  +L  
Sbjct: 105 NGTIQLVDLLSKLPRWQFSTGPPL---------SKHITTSKPDLNYVIYLDGSETSDLIE 164

Query: 141 IHTEYGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSS 200
           +H   G ++LP  ++E +  TPYI  D  V  GS+ +T F V+  +GE+I  H   +  +
Sbjct: 165 VHNGSG-VRLPWKLEEFIAETPYI-RDSFVTIGSKVSTTFVVNADSGEIIYKHSLPVALN 224

Query: 201 ELRNEGFGNYKSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLN-- 260
           E+         SK      D  +SG    +   + + RTDYS+ +S       +W+    
Sbjct: 225 EVGGPLVEEIPSK-----LDAARSGTSANI---IVVVRTDYSISASDLGEHLFNWTRTSF 284

Query: 261 ----VAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSG 320
                A  G   +          IP          +   +P S    A+V R        
Sbjct: 285 TANYYARYGHQDMLAQSSCLRGNIPCI--RTEGPPIKLYLPDSSSDNAIVLRP------- 344

Query: 321 PSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPM 380
                       ++ +S V A    LP +        ++          ++IA    VP 
Sbjct: 345 ------------VNEVSAVDALEPLLPPKKLPQPAGESNVALDSAQNQTADIALGHFVPA 404

Query: 381 PSVKINKSSIIQKQKMGTLAGVVGLFFVFLITMLVGLMKYGRTLAVR-VKQSLLKEKSFS 440
            +   N  S+ +          + L  +  +  L    KY R   +R +K  +  EK   
Sbjct: 405 DTELTN--SVTKFSYRWLFPTFLMLLIMACLVKLADASKYCRQFVIRFLKPFMRDEKLMD 464

Query: 441 TSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAG- 500
                  +SK+ K RK     NS +   +   E   T   +E    N  HD+    +   
Sbjct: 465 PRGKSEGTSKRRKARKKDGLINSTQIFSASDKEGNGTGGSTEAQ-SNKAHDSTNVELPNG 524

Query: 501 -SGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDR 560
            +GR+IGKL V +K+I  GSNGT++ EG Y GR VAVKRL+++H+D+ASKE++NLI SD+
Sbjct: 525 LNGRQIGKLCVYSKEIGKGSNGTVVFEGSYGGREVAVKRLLRSHNDIASKEIENLIASDQ 584

Query: 561 HPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHL 620
            PNIVR YG + D DFVY+SLERC CSL DLIQ++S  P ++  G +             
Sbjct: 585 DPNIVRMYGFEQDNDFVYISLERCRCSLADLIQLHSVPPFSNTKGTD------------- 644

Query: 621 ESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDL 680
                    + LW++ G PS+ LLKLM             RD+VAG+ HLH LGIIHRDL
Sbjct: 645 ---------IELWRQDGLPSAQLLKLM-------------RDVVAGIVHLHSLGIIHRDL 704

Query: 681 KPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVI 740
           KPQNVLI K+  + +KLSDMGISKRL  +++S+ H  T                      
Sbjct: 705 KPQNVLISKEGPLRAKLSDMGISKRLQEDMTSVSHHGT---------------------- 764

Query: 741 RDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIV 800
                  G GSSGWQAPEQL  GRQTRAIDLFSLGC++F+CIT G+HPFG+  ERD+ I+
Sbjct: 765 -------GFGSSGWQAPEQLRHGRQTRAIDLFSLGCLIFYCITKGKHPFGEYYERDMKII 824

Query: 801 KNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIE 860
            N+ +L +V++IPEAV LIS+LL+P+P  RP A  V+ HP FWS E+ LSFLRDTSDRIE
Sbjct: 825 NNQFDLFIVDHIPEAVHLISQLLDPDPEKRPTAVYVMHHPFFWSPELCLSFLRDTSDRIE 884

Query: 861 LEDRETNLLKALESTAPLALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNH 920
            +  ET+L+ ALE     A G  W +KL+   + ++GRYR+Y ++S RDLLR++RNK  H
Sbjct: 885 -KTSETDLIDALEGINVEAFGKNWGEKLDAALLADMGRYRKYSFESTRDLLRLIRNKSGH 888

Query: 921 YRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYF 963
           YRE   +++EL+GS+PEGF  YF++RFP+LLI+V++V+S  C+ EE F KYF
Sbjct: 945 YREFSDDLKELLGSLPEGFVQYFSSRFPKLLIKVYEVMSEHCKDEEAFSKYF 888

BLAST of HG10016295 vs. ExPASy Swiss-Prot
Match: Q9SF12 (Inactive serine/threonine-protein kinase/endoribonuclease IRE1-like OS=Arabidopsis thaliana OX=3702 GN=At3g11870 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 1.1e-80
Identity = 232/611 (37.97%), Postives = 320/611 (52.37%), Query Frame = 0

Query: 389 MGTLAGVVGLFFVFL----ITMLVGLMKYGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKN 448
           +G L  VV +F  F     +  +V   K  +  A RV  S        T N +V   KK+
Sbjct: 9   VGLLVVVVCVFLRFSKDKGLDGIVNEKKRDKNSAPRVSAS----GEDGTKNEQV--EKKS 68

Query: 449 KPR----KSKRSENSEKRDVSISSE--IEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGK 508
            P     +     NSE + +S+ S+  I  TL     SL    +D N T      RR   
Sbjct: 69  DPSGGLGEENEKTNSESKVLSVPSDQNINKTLPVMLPSLELRKYDENETPGKVVNRR--- 128

Query: 509 LFVTNKKIATGSNGTIILEGIYEGR-PVAVKRLVKTHHDVA--SKEVQNLIVSDRHPNIV 568
           L V+  ++  G NG  + +G+Y  R  VAVK L   H   A    E+ N  + D H NI+
Sbjct: 129 LLVSTNEMKYGRNGYEVFQGVYGRRSSVAVKCLDLAHTTEAFIQNEIDNHCLCDDHSNII 188

Query: 569 RWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKD 628
           R++G++ DQ F Y+ LE   CSLDDLI++       S+   +RDT    +    ++ ++ 
Sbjct: 189 RFHGLEQDQSFAYICLEPWKCSLDDLIKL-------SVRRTKRDT----QAVAPVDDLEK 248

Query: 629 AMPNLILWKEKGRPSSV--LLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQ 688
            M  +  WKEKG+P  +  +LKLM             RD+V GL HLH+L  IHR+L PQ
Sbjct: 249 VMKRIKFWKEKGKPLPLTPMLKLM-------------RDVVCGLAHLHKLKTIHRNLNPQ 308

Query: 689 NVL-IVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRD 748
           NVL IVK  ++ +K+SDM +SK L    SS  H AT                        
Sbjct: 309 NVLIIVKDMTLTAKISDMSLSKHLGGKKSSYKHLATCS---------------------- 368

Query: 749 KNPIVGCGSSGWQAPEQLLRGRQTR---AIDLFSLGCVLFFCITGGRHPFGDRLERDVNI 808
                  GSSGWQAPEQL + ++ +     D+F+ GC+L + +  G HPFG   ERD NI
Sbjct: 369 -------GSSGWQAPEQLNKDKKKKEDFPADMFNFGCLLHYAVM-GTHPFGSPSERDTNI 428

Query: 809 -VKNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDR 868
              NK NL LV N+ EA++LI +LLN  P LRP A++VL HPLFW SE RL FLR+ SDR
Sbjct: 429 KTNNKTNLSLVTNL-EAINLIEQLLNYKPDLRPSATQVLLHPLFWDSEKRLFFLREASDR 488

Query: 869 IELEDRETNLLKALESTAPLALG--TEWDKKLERIFITNIGRY-------------RRYK 928
           IEL+      L   ++ AP  LG   +W  KL + FIT+I                R YK
Sbjct: 489 IELDITMWGDLN--KTIAPRVLGESKDWASKLGKTFITHIENLAQAQPGQESRQYNRSYK 548

Query: 929 YDSVRDLLRVMRNKLNHYREL--PKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRF 963
           Y S+R LLR++RN L+H+RE+    +I+E++G +PEG D +FT RFP L++E++  IS  
Sbjct: 549 YWSLRHLLRLIRNILSHHREILDDPKIKEMVGKVPEGLDIFFTARFPNLMMEIYAFISMH 553

BLAST of HG10016295 vs. ExPASy Swiss-Prot
Match: Q09499 (Serine/threonine-protein kinase/endoribonuclease ire-1 OS=Caenorhabditis elegans OX=6239 GN=ire-1 PE=1 SV=2)

HSP 1 Score: 267.7 bits (683), Expect = 5.1e-70
Identity = 159/467 (34.05%), Postives = 244/467 (52.25%), Query Frame = 0

Query: 506 IATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQD 565
           + TG  GT++  G ++GR VAVKR+V      A +E   L  SD HP+++R++ M+SD  
Sbjct: 524 LGTGCEGTVVYRGTFDGREVAVKRVVSEFVKFAHREADLLRESDTHPHVIRYFCMESDSQ 583

Query: 566 FVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKE 625
           F YL+LE C  SL+D ++                              K+   N+ +   
Sbjct: 584 FRYLALELCIASLNDYVE-----------------------------QKEVQQNVTI--- 643

Query: 626 KGRPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIV---KQQS 685
                  L  +M+ A++             GL HLH   I+HRD+KPQNVLI    ++  
Sbjct: 644 ------ALRDIMKQATD-------------GLAHLHASKIVHRDMKPQNVLITMASQRGE 703

Query: 686 ICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSS 745
           + + +SD G+ KR+    +S+         S  I S L                   G+ 
Sbjct: 704 MRAVISDFGLCKRVQPGKNSI---------SRGIASGL------------------AGTD 763

Query: 746 GWQAPEQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNI 805
           GW APE L+    +  +D+FSLGC+ ++ +T G HPFG  L R  NIV  +  L  + ++
Sbjct: 764 GWIAPEVLISASTSYPVDIFSLGCIFYYVLTSGTHPFGKSLHRQANIVNGEYTLNKLADL 823

Query: 806 PE---AVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLL 865
            +   A DLIS +LN  P  R  A  VL HP FW+SE RL++  D SDR+E E+  + ++
Sbjct: 824 DDWSLADDLISSMLNVEPLHRLTADAVLNHPFFWTSEKRLAYFSDVSDRVEKEEDNSPVV 883

Query: 866 KALESTAPLALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQ 925
           + +E+ A + +   W +K+      ++ ++R YK  SVRDLLR MRNK +HYRELP++++
Sbjct: 884 RRIETDARIVVCGGWREKICDALKEDLRKFRTYKSFSVRDLLRAMRNKKHHYRELPEDVR 911

Query: 926 ELIGSIPEGFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHV 967
           + +G IP+ F  YFT+RFPRLL+ V+K  + +C  E  F++Y+   V
Sbjct: 944 QSLGDIPDQFLHYFTSRFPRLLLHVYKA-TEYCSGEAVFKRYYSDDV 911

BLAST of HG10016295 vs. ExPASy TrEMBL
Match: A0A1S3B291 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103485194 PE=4 SV=1)

HSP 1 Score: 1505.7 bits (3897), Expect = 0.0e+00
Identity = 779/940 (82.87%), Postives = 828/940 (88.09%), Query Frame = 0

Query: 30  FLILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDS 89
           FL+ + S +NS+FSVLNPYG NSEGV RIGGRSLLSLPLK KSSTALIAALDG IHLVDS
Sbjct: 19  FLVSSKSFENSEFSVLNPYGVNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGTIHLVDS 78

Query: 90  NSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKL 149
           NS+KIIWSFSSG PIYSSYQANINHEHNQENASGV  SFFFDCGDDWELYIHTE+GKMKL
Sbjct: 79  NSMKIIWSFSSGPPIYSSYQANINHEHNQENASGVGSSFFFDCGDDWELYIHTEHGKMKL 138

Query: 150 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNY 209
           PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVD VTGELIRNHMS  LSSEL NE  G+Y
Sbjct: 139 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGELIRNHMSKFLSSELSNEEPGSY 198

Query: 210 KSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPD 269
           KSK N+DIKDLMQS +MN VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPD
Sbjct: 199 KSKQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPD 258

Query: 270 VENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISN 329
           VENPIEGIPWTLQNNNSF +DFAMPLSCQSKALVFR+RS FLSGPSGYKRL SEAHN +N
Sbjct: 259 VENPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRSHFLSGPSGYKRLPSEAHNSNN 318

Query: 330 MSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQK 389
           +SLV ASGSFLPS+L V KHINT  E FML GP +N  S  VVP+PS+KIN+S+IIQ+QK
Sbjct: 319 ISLVSASGSFLPSQLTVGKHINTGSEKFMLTGPVNN-TSYRVVPLPSMKINESNIIQEQK 378

Query: 390 MGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPR 449
           MGTL G  GLFFVFL+TMLVGLMKYGRTLAV+VKQS LKE+ S  TSNSRV SSKKNKPR
Sbjct: 379 MGTLPGAFGLFFVFLLTMLVGLMKYGRTLAVKVKQSFLKEQSSLGTSNSRVISSKKNKPR 438

Query: 450 KSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIA 509
           KSK+S +S KR+VSISSEIED L+Q E +L NGFHDNNLTNIAGSGRR+GKL+VTNK+IA
Sbjct: 439 KSKKSGSSGKREVSISSEIEDMLLQREDNLNNGFHDNNLTNIAGSGRRVGKLWVTNKEIA 498

Query: 510 TGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFV 569
           TGSNGTIILEGIYEGRPVAVKRLVKTHHD+ SKEVQNLIVSDRHPNIVRWYGMDSDQDFV
Sbjct: 499 TGSNGTIILEGIYEGRPVAVKRLVKTHHDIGSKEVQNLIVSDRHPNIVRWYGMDSDQDFV 558

Query: 570 YLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKG 629
           YLSLERCTCSL DLI+IYS LPMNSMLGL++DTG MDEYN+HLESIK AMPNL LW E G
Sbjct: 559 YLSLERCTCSLYDLIKIYSDLPMNSMLGLDQDTGRMDEYNIHLESIKVAMPNLKLWNENG 618

Query: 630 RPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL 689
           RPSSVLLKLM             RD+VAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL
Sbjct: 619 RPSSVLLKLM-------------RDIVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL 678

Query: 690 SDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAP 749
           SDMGISKRLPANVSSLGHRAT                             GCGSSGWQAP
Sbjct: 679 SDMGISKRLPANVSSLGHRAT-----------------------------GCGSSGWQAP 738

Query: 750 EQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVD 809
           EQLL GRQTRAIDLFSLGCVLFFC+TGGRHPFGD LERDVNIV NKMNLLLV+NIPE VD
Sbjct: 739 EQLLHGRQTRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVD 798

Query: 810 LISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAP 869
           LIS+LLNPNPGLRPKASEVLQHPLFWS EMRLSFLRDTSDRIELEDRET+LLKALESTA 
Sbjct: 799 LISQLLNPNPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRETDLLKALESTAQ 858

Query: 870 LALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPE 929
           +ALG +W++KLE IFI NIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL+GSIP 
Sbjct: 859 IALGLKWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPV 915

Query: 930 GFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHVD 968
           GFDDYFT RFP+LLIEV+KVIS FCRQEECFQKYF+SHVD
Sbjct: 919 GFDDYFTRRFPKLLIEVYKVISCFCRQEECFQKYFKSHVD 915

BLAST of HG10016295 vs. ExPASy TrEMBL
Match: A0A0A0KJP8 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6G509680 PE=4 SV=1)

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 736/934 (78.80%), Postives = 791/934 (84.69%), Query Frame = 0

Query: 35  LSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDSNSLKI 94
           L S NS+ SVLN YG NSEGV RIGGRSLLSLPLK KSSTALIAALDGAIHLVDSNS+KI
Sbjct: 20  LVSSNSELSVLNLYGGNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGAIHLVDSNSMKI 79

Query: 95  IWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKLPSTID 154
           IWSFSSG PIYSSYQANINHE NQENASGV  SFFFDCGDDWELYIHTE+GKMKLPSTID
Sbjct: 80  IWSFSSGPPIYSSYQANINHEPNQENASGVGSSFFFDCGDDWELYIHTEHGKMKLPSTID 139

Query: 155 EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNYKSKNN 214
           EVVRNTPYIFEDGAVMTGSRKT VFEVD VTGELIRNHMS  LSS L NE   +YKSK+N
Sbjct: 140 EVVRNTPYIFEDGAVMTGSRKTAVFEVDLVTGELIRNHMSKFLSSGLSNEEQVSYKSKHN 199

Query: 215 LDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPDVENPI 274
           +DIKDLMQS  MN+VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPDVENPI
Sbjct: 200 MDIKDLMQS--MNSVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPDVENPI 259

Query: 275 EGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISNMSLVP 334
           EGIPWTLQNNNSF +D+ +PLSCQSKALVFR+RS FLSGPSGYK L SEAH+  NM    
Sbjct: 260 EGIPWTLQNNNSFGIDYGVPLSCQSKALVFRDRSHFLSGPSGYKILSSEAHDSDNM---- 319

Query: 335 ASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQKMGTLA 394
            SGSFLPS+LK+ KHIN     FM  G  +N  S AV P+PS+KIN+S+IIQKQKMG L 
Sbjct: 320 -SGSFLPSQLKIGKHINAKSGKFMFHGLVNN-TSYAVDPLPSMKINESNIIQKQKMGILP 379

Query: 395 GVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPRKSKRS 454
              GLFFVFL+TMLVGLM+YGRTL  +VKQ LLKEK S  TSNSR NSSKKNKPRK K+S
Sbjct: 380 EAFGLFFVFLLTMLVGLMRYGRTLTEKVKQFLLKEKLSLGTSNSRDNSSKKNKPRKLKKS 439

Query: 455 ENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATGSNG 514
             S KR+VSISSEIED L+Q E +L +GFH NNL     SGR IGKL++TNKKIATGSNG
Sbjct: 440 --SGKREVSISSEIEDMLLQRENNLNSGFHGNNLI----SGRHIGKLWITNKKIATGSNG 499

Query: 515 TIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLE 574
           T+ILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGM+SDQDFVYLSLE
Sbjct: 500 TVILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMESDQDFVYLSLE 559

Query: 575 RCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRPSSV 634
           RCTC+L DLIQIYS LP N +LGL+RD+GHMD YN+HLESIK A+PNL LW E GRPSS+
Sbjct: 560 RCTCNLYDLIQIYSDLPKNFVLGLDRDSGHMDGYNIHLESIKVALPNLKLWNENGRPSSI 619

Query: 635 LLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGI 694
           LLKLM             RD+V GL+HLHELGIIHRDLKPQNVLI+KQ+SICSKLSDMGI
Sbjct: 620 LLKLM-------------RDIVVGLKHLHELGIIHRDLKPQNVLILKQKSICSKLSDMGI 679

Query: 695 SKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQLLR 754
           SKRLPANVSSLGH AT                             GCGSSGWQAPEQLL 
Sbjct: 680 SKRLPANVSSLGHHAT-----------------------------GCGSSGWQAPEQLLH 739

Query: 755 GRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLISRL 814
            RQTRA+DLFSLGCVLFFCITGGRHPFGD LERDVNIV NKMNLLLV+NIPE VDLI RL
Sbjct: 740 ERQTRAVDLFSLGCVLFFCITGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVDLICRL 799

Query: 815 LNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAPLALGT 874
           LNPNPGLRPKAS+VLQHPLFWS EMRLSFLRDTSDRIELEDRE+NLLKALESTA +ALG 
Sbjct: 800 LNPNPGLRPKASKVLQHPLFWSPEMRLSFLRDTSDRIELEDRESNLLKALESTAQIALGI 859

Query: 875 EWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGFDDY 934
           +W++KLE IFI NIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL+GSIPEGFDDY
Sbjct: 860 KWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPEGFDDY 897

Query: 935 FTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHV 967
           FTTRFP+LLIEV+KV S FCR EECF+KYF+SHV
Sbjct: 920 FTTRFPKLLIEVYKVTSCFCRHEECFKKYFKSHV 897

BLAST of HG10016295 vs. ExPASy TrEMBL
Match: A0A6J1BYY0 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111005957 PE=4 SV=1)

HSP 1 Score: 1375.9 bits (3560), Expect = 0.0e+00
Identity = 705/959 (73.51%), Postives = 800/959 (83.42%), Query Frame = 0

Query: 19  LLTITWRSPVGFLILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIA 78
           L+ + +RS +G    + S +NS+FSVL+PYGDNSEG+AR GGRSLLSLPLK K +TAL+A
Sbjct: 14  LIILRFRSSIG---ESRSLENSKFSVLSPYGDNSEGLARTGGRSLLSLPLKGKINTALVA 73

Query: 79  ALDGAIHLVDSNSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWEL 138
           A DGAIHLVDSNS+  IWSF+SG PIYSSYQA+I H+HNQENASG+S SFFFDCGDDWEL
Sbjct: 74  ASDGAIHLVDSNSMNKIWSFTSGPPIYSSYQAHIEHDHNQENASGLSSSFFFDCGDDWEL 133

Query: 139 YIHTEYGKMKLPSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLS 198
           Y+HTE+GKMKLPS+IDEVVRNTPYIFEDGAVM GSRKTTVFEVDPVTG+LIRNH+S+  S
Sbjct: 134 YMHTEHGKMKLPSSIDEVVRNTPYIFEDGAVMIGSRKTTVFEVDPVTGKLIRNHISDFSS 193

Query: 199 SELRNEGFGNYKSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNV 258
           SEL NE   +Y SK NLD KDL+Q GV++ VEPRLYITRTDYSLKSSFS SEEV+WSLNV
Sbjct: 194 SELSNEEHSSYNSKQNLDNKDLIQPGVVSPVEPRLYITRTDYSLKSSFSGSEEVAWSLNV 253

Query: 259 AEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYK 318
           AEIGATLLCP+VENP + +PW LQ+N SF +DFAMPLSCQSK +VFRERS   SG SGYK
Sbjct: 254 AEIGATLLCPEVENPTKSVPWNLQSNGSFNLDFAMPLSCQSKVVVFRERSHVFSGSSGYK 313

Query: 319 RLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASP--------AV 378
           RLSEAHNI NMSLVPASG  LPS+LKV +  NTHPE  MLPGPA NI+SP        AV
Sbjct: 314 RLSEAHNIGNMSLVPASGPTLPSQLKVDEQQNTHPEKLMLPGPAPNISSPLPPNSSSKAV 373

Query: 379 VPMPSVKINKSSIIQKQKMGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEKS 438
           +PMP +KIN+SS++Q  KMGTL G  GLFFVFL+T+LVGL+ + R L  +VKQ  L+EK 
Sbjct: 374 IPMPLMKINESSMVQGHKMGTLNGPFGLFFVFLVTILVGLVTFSRGLTGKVKQFFLREKL 433

Query: 439 FSTSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIA 498
            STSNS++ SSKKNK RKSKR+ NS+KRD+S+SSE ED LM SEG++ N FH +NL + A
Sbjct: 434 SSTSNSKILSSKKNKARKSKRNVNSDKRDISVSSENEDMLMPSEGNVNNWFHHSNLPDNA 493

Query: 499 GSGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDR 558
           G+GRRIGKLFVTNK+IA GSNGTI+LEG+YEGRPVAVKRLVKTHHDVA KEVQNLI SDR
Sbjct: 494 GTGRRIGKLFVTNKEIARGSNGTIVLEGVYEGRPVAVKRLVKTHHDVAFKEVQNLIASDR 553

Query: 559 HPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHL 618
           HPNIVRWYG++SDQDFVYLSLERCTCSLDDLIQI S    NSM GL++DT  M +Y LHL
Sbjct: 554 HPNIVRWYGVESDQDFVYLSLERCTCSLDDLIQICSDQSFNSMPGLDQDTEPMIKYRLHL 613

Query: 619 ESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDL 678
           ES+KD MP+L LWKE G PSSVLLKLM             RDMVAG+EHLHELGIIHRDL
Sbjct: 614 ESVKDVMPDLKLWKENGHPSSVLLKLM-------------RDMVAGVEHLHELGIIHRDL 673

Query: 679 KPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVI 738
           KPQNVLI+KQ+S+C+KLSDMGISKRL A++SSLGH AT                      
Sbjct: 674 KPQNVLIIKQKSMCAKLSDMGISKRLLADMSSLGHHAT---------------------- 733

Query: 739 RDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIV 798
                  GCGSSGWQAPEQLL GRQTRA+DLFSLGCVLFFCITGGRHPFGDRLERDVNIV
Sbjct: 734 -------GCGSSGWQAPEQLLHGRQTRAVDLFSLGCVLFFCITGGRHPFGDRLERDVNIV 793

Query: 799 KNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIE 858
           KN+M+L LV +IPEAVDLIS+LLNP+P LRPKASEVLQHPLFWSSE RLSFLRDTSDR+E
Sbjct: 794 KNQMDLFLVQSIPEAVDLISQLLNPDPDLRPKASEVLQHPLFWSSETRLSFLRDTSDRVE 853

Query: 859 LEDR--ETNLLKALESTAPLALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKL 918
           LEDR  E+ LLKALE TA LALGT+WD+K+E IFITNIGRYRRYKYDSVRDLLRVMRNKL
Sbjct: 854 LEDRESESGLLKALEKTAQLALGTKWDEKMEPIFITNIGRYRRYKYDSVRDLLRVMRNKL 913

Query: 919 NHYRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHVD 968
           NHYRELP EIQELIGS+PEGFD YF +RFPRLLIEV+K+IS++CRQEECFQKYF+SH++
Sbjct: 914 NHYRELPNEIQELIGSVPEGFDGYFASRFPRLLIEVYKIISQYCRQEECFQKYFKSHIE 927

BLAST of HG10016295 vs. ExPASy TrEMBL
Match: A0A5D3CM22 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00570 PE=4 SV=1)

HSP 1 Score: 1353.6 bits (3502), Expect = 0.0e+00
Identity = 711/879 (80.89%), Postives = 760/879 (86.46%), Query Frame = 0

Query: 32  ILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDSNS 91
           +L L S + +FSVLNPYG NSEGV RIGGRSLLSLPLK KSSTALIAALDG IHLVDSNS
Sbjct: 1   MLILVSYSLEFSVLNPYGVNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGTIHLVDSNS 60

Query: 92  LKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKLPS 151
           +KIIWSFSSG PIYSSYQANINHEHNQENASGV  SFFFDCGDDWELYIHTE+GKMKLPS
Sbjct: 61  MKIIWSFSSGPPIYSSYQANINHEHNQENASGVGSSFFFDCGDDWELYIHTEHGKMKLPS 120

Query: 152 TIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNYKS 211
           TIDEVVRNTPYIFEDGAVMTGSRKTTVFEVD VTGELIRNHMS  LSSEL NE  G+YKS
Sbjct: 121 TIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGELIRNHMSKFLSSELSNEEPGSYKS 180

Query: 212 KNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPDVE 271
           K N+DIKDLMQS +MN VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPDVE
Sbjct: 181 KQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPDVE 240

Query: 272 NPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISNMS 331
           NPIEGIPWTLQNNNSF +DFAMPLSCQSKALVFR+RS FLSGPSGYKRL SEAHN +N+S
Sbjct: 241 NPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRSHFLSGPSGYKRLPSEAHNSNNIS 300

Query: 332 LVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQKMG 391
           LV ASGSFLPS+L V KHINT  E FMLPGP +N  S  VVP+PS+KIN+S+IIQ+QKMG
Sbjct: 301 LVSASGSFLPSQLTVGKHINTGSEKFMLPGPVNN-TSYRVVPLPSMKINESNIIQEQKMG 360

Query: 392 TLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPRKS 451
           TL G  GLFFVFL+TMLVGLMKYGRTLAV+VKQS LKE+ S  TSNSRV SSKKNKPRKS
Sbjct: 361 TLPGAFGLFFVFLLTMLVGLMKYGRTLAVKVKQSFLKEQSSLGTSNSRVISSKKNKPRKS 420

Query: 452 KRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATG 511
           K+S +S KR+VSISSEIED L+Q E +L NGFHDNNLTNIAGSGRR+GKL+VTNK+IATG
Sbjct: 421 KKSGSSGKREVSISSEIEDMLLQREDNLNNGFHDNNLTNIAGSGRRVGKLWVTNKEIATG 480

Query: 512 SNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL 571
           SNGTIILEGIYEGRPVAVKRLVKTHHD+ SKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL
Sbjct: 481 SNGTIILEGIYEGRPVAVKRLVKTHHDIGSKEVQNLIVSDRHPNIVRWYGMDSDQDFVYL 540

Query: 572 SLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRP 631
           SLERCTCSL DLI+IYS LPMNSMLGL++DTG MDEYN+HLESIK AMPNL LW E GRP
Sbjct: 541 SLERCTCSLYDLIKIYSDLPMNSMLGLDQDTGRMDEYNIHLESIKVAMPNLKLWNENGRP 600

Query: 632 SSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD 691
           SSVLLKLM             RD+VAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD
Sbjct: 601 SSVLLKLM-------------RDIVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSD 660

Query: 692 MGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQ 751
           MGISKRLPANVSSLGHRAT                             GCGSSGWQAPEQ
Sbjct: 661 MGISKRLPANVSSLGHRAT-----------------------------GCGSSGWQAPEQ 720

Query: 752 LLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLI 811
           LL GRQTRAIDLFSLGCVLFFC+TGGRHPFGD LERDVNIV NKMNLLLV+NIPE VDLI
Sbjct: 721 LLHGRQTRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVDLI 780

Query: 812 SRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAPLA 871
           S+LLNPNPGLRPKASEVLQHPLFWS EMRLSFLRDTSDRIELEDRET+LLKALESTA +A
Sbjct: 781 SQLLNPNPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRETDLLKALESTAQIA 836

Query: 872 LGTEWDKKLERIFITNIGR--YRRYKYDSVRDLLRVMRN 907
           LG +W++KLE IFI NIGR    R+++D +  LL   +N
Sbjct: 841 LGLKWNEKLEPIFIANIGRSLLDRFRWDLMTTLLEGFQN 836

BLAST of HG10016295 vs. ExPASy TrEMBL
Match: A0A1S3B279 (serine/threonine-protein kinase/endoribonuclease IRE1b-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103485194 PE=4 SV=1)

HSP 1 Score: 1333.2 bits (3449), Expect = 0.0e+00
Identity = 709/940 (75.43%), Postives = 754/940 (80.21%), Query Frame = 0

Query: 30  FLILNLSSDNSQFSVLNPYGDNSEGVARIGGRSLLSLPLKDKSSTALIAALDGAIHLVDS 89
           FL+ + S +NS+FSVLNPYG NSEGV RIGGRSLLSLPLK KSSTALIAALDG IHLVDS
Sbjct: 19  FLVSSKSFENSEFSVLNPYGVNSEGVTRIGGRSLLSLPLKGKSSTALIAALDGTIHLVDS 78

Query: 90  NSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIHTEYGKMKL 149
           NS+KIIWSFSSG PIYSSYQANINHEHNQENASGV  SFFFDCGDDWELYIHTE+GKMKL
Sbjct: 79  NSMKIIWSFSSGPPIYSSYQANINHEHNQENASGVGSSFFFDCGDDWELYIHTEHGKMKL 138

Query: 150 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNHMSNLLSSELRNEGFGNY 209
           PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVD VTGELIRNHMS  LSSEL NE  G+Y
Sbjct: 139 PSTIDEVVRNTPYIFEDGAVMTGSRKTTVFEVDLVTGELIRNHMSKFLSSELSNEEPGSY 198

Query: 210 KSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEVSWSLNVAEIGATLLCPD 269
           KSK N+DIKDLMQS +MN VEPRLYITRTDYSLKSSFSNSEE SWSLNVAEIGATLLCPD
Sbjct: 199 KSKQNMDIKDLMQSSMMNPVEPRLYITRTDYSLKSSFSNSEEASWSLNVAEIGATLLCPD 258

Query: 270 VENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLSGPSGYKRL-SEAHNISN 329
           VENPIEGIPWTLQNNNSF +DFAMPLSCQSKALVFR+RS FLSGPSGYKRL SEAHN +N
Sbjct: 259 VENPIEGIPWTLQNNNSFGIDFAMPLSCQSKALVFRDRSHFLSGPSGYKRLPSEAHNSNN 318

Query: 330 MSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVPMPSVKINKSSIIQKQK 389
           +SLV ASGSFLPS+L V KHINT  E FML GP +N  S  VVP+PS+KIN+S+IIQ+QK
Sbjct: 319 ISLVSASGSFLPSQLTVGKHINTGSEKFMLTGPVNN-TSYRVVPLPSMKINESNIIQEQK 378

Query: 390 MGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEK-SFSTSNSRVNSSKKNKPR 449
           MGTL G  GLFFVFL+TMLVGLMKYGRTLAV+VKQS LKE+ S  TSNSRV SSKKNKPR
Sbjct: 379 MGTLPGAFGLFFVFLLTMLVGLMKYGRTLAVKVKQSFLKEQSSLGTSNSRVISSKKNKPR 438

Query: 450 KSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIA 509
           KSK+S +S KR+VSISSEIED L+Q E +L NGFHDNNLTNIAGSGRR+GKL+VTNK+IA
Sbjct: 439 KSKKSGSSGKREVSISSEIEDMLLQREDNLNNGFHDNNLTNIAGSGRRVGKLWVTNKEIA 498

Query: 510 TGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFV 569
           TGSNGTIILEGIYEGRPVAVKRLVKTHHD+ SKEVQNLIVSDRHPNIVRW          
Sbjct: 499 TGSNGTIILEGIYEGRPVAVKRLVKTHHDIGSKEVQNLIVSDRHPNIVRW---------- 558

Query: 570 YLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKG 629
                                                                       
Sbjct: 559 ------------------------------------------------------------ 618

Query: 630 RPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL 689
                                   D+VAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL
Sbjct: 619 ------------------------DIVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKL 678

Query: 690 SDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAP 749
           SDMGISKRLPANVSSLGHRAT                             GCGSSGWQAP
Sbjct: 679 SDMGISKRLPANVSSLGHRAT-----------------------------GCGSSGWQAP 738

Query: 750 EQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVD 809
           EQLL GRQTRAIDLFSLGCVLFFC+TGGRHPFGD LERDVNIV NKMNLLLV+NIPE VD
Sbjct: 739 EQLLHGRQTRAIDLFSLGCVLFFCVTGGRHPFGDSLERDVNIVNNKMNLLLVDNIPEVVD 798

Query: 810 LISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELEDRETNLLKALESTAP 869
           LIS+LLNPNPGLRPKASEVLQHPLFWS EMRLSFLRDTSDRIELEDRET+LLKALESTA 
Sbjct: 799 LISQLLNPNPGLRPKASEVLQHPLFWSPEMRLSFLRDTSDRIELEDRETDLLKALESTAQ 834

Query: 870 LALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPE 929
           +ALG +W++KLE IFI NIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQEL+GSIP 
Sbjct: 859 IALGLKWNEKLEPIFIANIGRYRRYKYDSVRDLLRVMRNKLNHYRELPKEIQELVGSIPV 834

Query: 930 GFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHVD 968
           GFDDYFT RFP+LLIEV+KVIS FCRQEECFQKYF+SHVD
Sbjct: 919 GFDDYFTRRFPKLLIEVYKVISCFCRQEECFQKYFKSHVD 834

BLAST of HG10016295 vs. TAIR 10
Match: AT2G17520.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 651.7 bits (1680), Expect = 9.0e-187
Identity = 417/956 (43.62%), Postives = 554/956 (57.95%), Query Frame = 0

Query: 30  FLILNLSSDNSQFSVLNPYGDNSEGV------ARIGGRSLLSLPLK--DKSSTALIAALD 89
           FL+L LS       +++P G  ++ V      +  G RS+L +  +   + +T L+   D
Sbjct: 13  FLLLLLSP-----WIMSPCGGAADDVTYPIVPSSPGRRSILQIRREPPTEPNTKLVVDRD 72

Query: 90  GAIHLVDSNSLKIIWSFSSGLPIYSSYQANINHEHNQENASGVSRSFFFDCGDDWELYIH 149
           G + L         WSFS+G P++S YQA  N  +N ENA+ ++R            +I 
Sbjct: 73  GKVFLKQQPKETPYWSFSTGSPMHSLYQAPAN--NNTENATEITRP-----------HII 132

Query: 150 TEY-GKMKLPSTID--------EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGELIRNH 209
            EY    K  +T+D        E  R  P + +DG V  GS  T+ + VD  +G LI  +
Sbjct: 133 VEYLNNSKAATTVDGYHNWTVQEFFRQKPLVTDDG-VTLGSETTSAYLVDGRSGRLIHVY 192

Query: 210 MSNLLSSELRNEGFGNYKSKNNLDIKDLMQSGVMNTVEPRLYITRTDYSLKSSFSNSEEV 269
            S            G+ K  N L +K       +N  EP L I RTD  L+     + ++
Sbjct: 193 KST-----------GDTKITNAL-VKPASTEDFVN--EP-LLIRRTDSKLEHFSKTTGKL 252

Query: 270 SWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKALVFRERSQFLS 329
            W+L V+   A LLC  V N    +   LQ          MPL C S+  V         
Sbjct: 253 VWNLTVSHFRAALLCDPVFNSGYDLGPKLQTG------IYMPLLCGSQIDV--------R 312

Query: 330 GPSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPGPASNIASPAVVP 389
           GP    R+                   P  +K            MLP P+ N        
Sbjct: 313 GPEIVIRVLHDQ---------------PMNVK------------MLPSPSLNHFESENSI 372

Query: 390 MPSVKINKSSIIQKQKMGTLAGVVGLFFVFLITMLVGLMKYGRTLAVRVKQSLLKEKSFS 449
           MP  K  +S  +Q+Q       + G +    + +L  L+  G  ++V +K+   +    S
Sbjct: 373 MPFGKARESRKLQEQHKQKYTYLFGQWSP--VKLLAPLVLLGVVVSVFIKKFSSRGSDVS 432

Query: 450 TSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIEDTLMQSEGSLYNGFHDNNLTNIAGS 509
               +   SKK K RKS +  N +    S+    +   +   G +  GF  NN  + A  
Sbjct: 433 L---KAGPSKKKKNRKSAKDTNRQ----SVPRGQDQFELIEGGQMLLGF--NNFQSGATD 492

Query: 510 GRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLVKTHHDVASKEVQNLIVSDRHP 569
           GR+IGKLF+++K+IA GSNGT++ EGIYEGRPVAVKRLV++HH+VA KE+QNLI SD+H 
Sbjct: 493 GRKIGKLFLSSKEIAKGSNGTVVFEGIYEGRPVAVKRLVRSHHEVAFKEIQNLIASDQHT 552

Query: 570 NIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLES 629
           NI+RWYG++ DQDFVYLSLERCTCSLDDLI+ Y    M  +L     T  +  Y + L+S
Sbjct: 553 NIIRWYGVEYDQDFVYLSLERCTCSLDDLIKSYLEFSMTKVLENNDSTEGVAAYKIQLDS 612

Query: 630 IKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKP 689
           ++  +     WK  G PS ++LKLM             RD+V G+ HLHELGI+HRDLKP
Sbjct: 613 LEGVIKGNNFWKVGGHPSPLMLKLM-------------RDIVCGIVHLHELGIVHRDLKP 672

Query: 690 QNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRD 749
           QNVLI K  ++ +KLSDMGISKR+  ++SSLGH ATG                       
Sbjct: 673 QNVLISKDMTLSAKLSDMGISKRMSRDMSSLGHLATGS---------------------- 732

Query: 750 KNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFCITGGRHPFGDRLERDVNIVKN 809
                  GSSGWQAPEQLL+GRQTRA+D+FSLGCV+F+ ITG +HPFGD LERDVNIVKN
Sbjct: 733 -------GSSGWQAPEQLLQGRQTRAVDMFSLGCVIFYTITGCKHPFGDDLERDVNIVKN 792

Query: 810 KMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDRIELE 869
           K++L LV ++PEA DLISRLLNP+P LRP A+EVL HP+FW+SEMRLSFLRD SDR+ELE
Sbjct: 793 KVDLFLVEHVPEASDLISRLLNPDPDLRPSATEVLLHPMFWNSEMRLSFLRDASDRVELE 840

Query: 870 DRE--TNLLKALESTAPLALGTEWDKKLERIFITNIGRYRRYKYDSVRDLLRVMRNKLNH 929
           +RE  + +LKA+ESTAP+A+G +WD+KLE +FITNIGRYRRYKYDS+RDLLRV+RNKLNH
Sbjct: 853 NREADSEILKAMESTAPVAIGGKWDEKLEPVFITNIGRYRRYKYDSIRDLLRVIRNKLNH 840

Query: 930 YRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRFCRQEECFQKYFRSHV 967
           +RELP EIQEL+G++PEGFD+YF  RFP+LLIEV++VIS  CR+EE F+KYF+  +
Sbjct: 913 HRELPPEIQELVGTVPEGFDEYFAVRFPKLLIEVYRVISLHCREEEVFRKYFKCDI 840

BLAST of HG10016295 vs. TAIR 10
Match: AT5G24360.1 (inositol requiring 1-1 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.8e-179
Identity = 391/917 (42.64%), Postives = 546/917 (59.54%), Query Frame = 0

Query: 71  KSSTALIAALDGAIHLVDSNSLKIIWSFSSGLPIYSSYQA-NINHEHNQENASGVSRSFF 130
           +S   L++ +DG+I LVD +S K+ W+F +  PIYSSYQA + ++  ++E +S +   F+
Sbjct: 45  ESGYVLVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFY 104

Query: 131 FDCGDDWELYIHTEYGKMKLPSTID--EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGE 190
            DC  DW LY  +     ++   +D  E +   PY   D  V+ G + T+VF +D  TG+
Sbjct: 105 MDCDKDWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGK 164

Query: 191 LIRNHMSNLLSSELRNEGFGNYKSKNNLDIKD---LMQSGVMNTVE-PRL-YITRTDYSL 250
           L++ +  + L S   N    N K K  +  K+   L  SG   + + P L YI R D+ +
Sbjct: 165 LVKRYRMDELYS---NTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKI 224

Query: 251 KSSFSNSEEVSWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKAL 310
           +   S   +V WS++ A++ A L   +    I G+  ++  N         PLS  +   
Sbjct: 225 Q-CISKFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVP 284

Query: 311 VFRERSQFLSGPSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPG-- 370
           + + R+        Y+ L       + +L        P++L +            LPG  
Sbjct: 285 MVQLRN------VKYETLFPRLGFLDEALYLPFQDRKPNQLAI-----GDGNQLTLPGNK 344

Query: 371 PASNIASPAVVPMPSVKINK------SSIIQKQKMGTLAGVVGLFFVFLITML--VGLMK 430
            A  + S   +P+P   I++       S  Q       +G++ L F F +TML   GL  
Sbjct: 345 EAEEVLS---LPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFF 404

Query: 431 YGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIED-TLM 490
           Y    ++R+K+  + E   +T   +   SKKN   K+      +K +  IS   +D +  
Sbjct: 405 YRLRQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVH----KKENGFISGGNKDPSHE 464

Query: 491 QSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLV 550
           ++E  L   F    L N +  G R+GKLFV+NK+IA GSNGT++LEG YEGR VAVKRLV
Sbjct: 465 ENEKRLLTAF--PGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRLV 524

Query: 551 KTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMN 610
           ++HHDVA KE+ NL+ SD+H NIVRWYG+D D+ F+Y+SLE C CSL+DLI   S L   
Sbjct: 525 QSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASSAL--- 584

Query: 611 SMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYR 670
               LE        +++ +  I +    + LWKE G PS VLLKLM             R
Sbjct: 585 ----LESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLM-------------R 644

Query: 671 DMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKY 730
           D+VAGL HLH++GI+HRDLKPQNVLIVK  S+C+KLSDMGISKRLPA+ S+L   +TG  
Sbjct: 645 DIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG-- 704

Query: 731 PSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFC 790
                                    +G GSSGWQAPEQL   RQTRA+DLFSLGCVLFFC
Sbjct: 705 -------------------------LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFC 764

Query: 791 ITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPL 850
           +TGG+HP+GD  ERDVN++ ++ +L L+ ++PEAV L++ LLNP+P LRP+A +V+ HPL
Sbjct: 765 MTGGKHPYGDNYERDVNVLNDQKDLFLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPL 824

Query: 851 FWSSEMRLSFLRDTSDRIELEDRE--TNLLKALESTAPLALGTEWDKKLERIFITNIGRY 910
           FW+S+MRLSFLRD SDR+ELE+RE  + LL ALESTA + L   WD+KL+ IF+ NIGRY
Sbjct: 825 FWNSDMRLSFLRDASDRVELENREEGSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRY 880

Query: 911 RRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVIS 967
           RRYK+DS+RDLLRV+RNKLNHYRELPKE+QEL+GS+PEGF+ YF++RFP+LLI+V+ V+ 
Sbjct: 885 RRYKFDSIRDLLRVIRNKLNHYRELPKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLF 880

BLAST of HG10016295 vs. TAIR 10
Match: AT5G24360.2 (inositol requiring 1-1 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.8e-179
Identity = 391/917 (42.64%), Postives = 546/917 (59.54%), Query Frame = 0

Query: 71  KSSTALIAALDGAIHLVDSNSLKIIWSFSSGLPIYSSYQA-NINHEHNQENASGVSRSFF 130
           +S   L++ +DG+I LVD +S K+ W+F +  PIYSSYQA + ++  ++E +S +   F+
Sbjct: 51  ESGYVLVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFY 110

Query: 131 FDCGDDWELYIHTEYGKMKLPSTID--EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGE 190
            DC  DW LY  +     ++   +D  E +   PY   D  V+ G + T+VF +D  TG+
Sbjct: 111 MDCDKDWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGK 170

Query: 191 LIRNHMSNLLSSELRNEGFGNYKSKNNLDIKD---LMQSGVMNTVE-PRL-YITRTDYSL 250
           L++ +  + L S   N    N K K  +  K+   L  SG   + + P L YI R D+ +
Sbjct: 171 LVKRYRMDELYS---NTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKI 230

Query: 251 KSSFSNSEEVSWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKAL 310
           +   S   +V WS++ A++ A L   +    I G+  ++  N         PLS  +   
Sbjct: 231 Q-CISKFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVP 290

Query: 311 VFRERSQFLSGPSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPG-- 370
           + + R+        Y+ L       + +L        P++L +            LPG  
Sbjct: 291 MVQLRN------VKYETLFPRLGFLDEALYLPFQDRKPNQLAI-----GDGNQLTLPGNK 350

Query: 371 PASNIASPAVVPMPSVKINK------SSIIQKQKMGTLAGVVGLFFVFLITML--VGLMK 430
            A  + S   +P+P   I++       S  Q       +G++ L F F +TML   GL  
Sbjct: 351 EAEEVLS---LPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFF 410

Query: 431 YGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIED-TLM 490
           Y    ++R+K+  + E   +T   +   SKKN   K+      +K +  IS   +D +  
Sbjct: 411 YRLRQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVH----KKENGFISGGNKDPSHE 470

Query: 491 QSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLV 550
           ++E  L   F    L N +  G R+GKLFV+NK+IA GSNGT++LEG YEGR VAVKRLV
Sbjct: 471 ENEKRLLTAF--PGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRLV 530

Query: 551 KTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMN 610
           ++HHDVA KE+ NL+ SD+H NIVRWYG+D D+ F+Y+SLE C CSL+DLI   S L   
Sbjct: 531 QSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASSAL--- 590

Query: 611 SMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYR 670
               LE        +++ +  I +    + LWKE G PS VLLKLM             R
Sbjct: 591 ----LESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLM-------------R 650

Query: 671 DMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKY 730
           D+VAGL HLH++GI+HRDLKPQNVLIVK  S+C+KLSDMGISKRLPA+ S+L   +TG  
Sbjct: 651 DIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG-- 710

Query: 731 PSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFC 790
                                    +G GSSGWQAPEQL   RQTRA+DLFSLGCVLFFC
Sbjct: 711 -------------------------LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFC 770

Query: 791 ITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPL 850
           +TGG+HP+GD  ERDVN++ ++ +L L+ ++PEAV L++ LLNP+P LRP+A +V+ HPL
Sbjct: 771 MTGGKHPYGDNYERDVNVLNDQKDLFLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPL 830

Query: 851 FWSSEMRLSFLRDTSDRIELEDRE--TNLLKALESTAPLALGTEWDKKLERIFITNIGRY 910
           FW+S+MRLSFLRD SDR+ELE+RE  + LL ALESTA + L   WD+KL+ IF+ NIGRY
Sbjct: 831 FWNSDMRLSFLRDASDRVELENREEGSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRY 886

Query: 911 RRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVIS 967
           RRYK+DS+RDLLRV+RNKLNHYRELPKE+QEL+GS+PEGF+ YF++RFP+LLI+V+ V+ 
Sbjct: 891 RRYKFDSIRDLLRVIRNKLNHYRELPKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLF 886

BLAST of HG10016295 vs. TAIR 10
Match: AT5G24360.3 (inositol requiring 1-1 )

HSP 1 Score: 627.5 bits (1617), Expect = 1.8e-179
Identity = 391/917 (42.64%), Postives = 546/917 (59.54%), Query Frame = 0

Query: 71  KSSTALIAALDGAIHLVDSNSLKIIWSFSSGLPIYSSYQA-NINHEHNQENASGVSRSFF 130
           +S   L++ +DG+I LVD +S K+ W+F +  PIYSSYQA + ++  ++E +S +   F+
Sbjct: 31  ESGYVLVSTVDGSISLVDMSSQKLDWTFHTNEPIYSSYQAPHYHYTTDEERSSVLGDDFY 90

Query: 131 FDCGDDWELYIHTEYGKMKLPSTID--EVVRNTPYIFEDGAVMTGSRKTTVFEVDPVTGE 190
            DC  DW LY  +     ++   +D  E +   PY   D  V+ G + T+VF +D  TG+
Sbjct: 91  MDCDKDWRLYNSSVRKGKRVNEIVDASEFIGTLPYTSTDRIVL-GKKDTSVFLLDWKTGK 150

Query: 191 LIRNHMSNLLSSELRNEGFGNYKSKNNLDIKD---LMQSGVMNTVE-PRL-YITRTDYSL 250
           L++ +  + L S   N    N K K  +  K+   L  SG   + + P L YI R D+ +
Sbjct: 151 LVKRYRMDELYS---NTVVENDKEKAIVLSKEAPLLFGSGFKKSEDFPELVYIERKDFKI 210

Query: 251 KSSFSNSEEVSWSLNVAEIGATLLCPDVENPIEGIPWTLQNNNSFEVDFAMPLSCQSKAL 310
           +   S   +V WS++ A++ A L   +    I G+  ++  N         PLS  +   
Sbjct: 211 Q-CISKFGDVLWSVSYAKMEAKLQNHESVQFISGLSSSVGKNQ-------FPLSYTTSVP 270

Query: 311 VFRERSQFLSGPSGYKRLSEAHNISNMSLVPASGSFLPSELKVCKHINTHPETFMLPG-- 370
           + + R+        Y+ L       + +L        P++L +            LPG  
Sbjct: 271 MVQLRN------VKYETLFPRLGFLDEALYLPFQDRKPNQLAI-----GDGNQLTLPGNK 330

Query: 371 PASNIASPAVVPMPSVKINK------SSIIQKQKMGTLAGVVGLFFVFLITML--VGLMK 430
            A  + S   +P+P   I++       S  Q       +G++ L F F +TML   GL  
Sbjct: 331 EAEEVLS---LPLPETVISQITDIIDGSTKQAGFASKFSGLIVLIFGFCVTMLSVCGLFF 390

Query: 431 YGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKNKPRKSKRSENSEKRDVSISSEIED-TLM 490
           Y    ++R+K+  + E   +T   +   SKKN   K+      +K +  IS   +D +  
Sbjct: 391 YRLRQSIRIKEPYVSEVPIATPKKK--KSKKNGTTKAVH----KKENGFISGGNKDPSHE 450

Query: 491 QSEGSLYNGFHDNNLTNIAGSGRRIGKLFVTNKKIATGSNGTIILEGIYEGRPVAVKRLV 550
           ++E  L   F    L N +  G R+GKLFV+NK+IA GSNGT++LEG YEGR VAVKRLV
Sbjct: 451 ENEKRLLTAF--PGLNNSSAEGYRVGKLFVSNKEIAKGSNGTVVLEGSYEGRLVAVKRLV 510

Query: 551 KTHHDVASKEVQNLIVSDRHPNIVRWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMN 610
           ++HHDVA KE+ NL+ SD+H NIVRWYG+D D+ F+Y+SLE C CSL+DLI   S L   
Sbjct: 511 QSHHDVAQKEILNLMASDKHSNIVRWYGVDQDEHFIYISLELCACSLNDLIYASSAL--- 570

Query: 611 SMLGLERDTGHMDEYNLHLESIKDAMPNLILWKEKGRPSSVLLKLMRYASEPKIWLVKYR 670
               LE        +++ +  I +    + LWKE G PS VLLKLM             R
Sbjct: 571 ----LESPMASSSIHSIQINPIFENGKGVELWKENGHPSPVLLKLM-------------R 630

Query: 671 DMVAGLEHLHELGIIHRDLKPQNVLIVKQQSICSKLSDMGISKRLPANVSSLGHRATGKY 730
           D+VAGL HLH++GI+HRDLKPQNVLIVK  S+C+KLSDMGISKRLPA+ S+L   +TG  
Sbjct: 631 DIVAGLVHLHDIGIVHRDLKPQNVLIVKNSSLCAKLSDMGISKRLPADTSALTRNSTG-- 690

Query: 731 PSIAIISQLQYSYYYKWVIRDKNPIVGCGSSGWQAPEQLLRGRQTRAIDLFSLGCVLFFC 790
                                    +G GSSGWQAPEQL   RQTRA+DLFSLGCVLFFC
Sbjct: 691 -------------------------LGSGSSGWQAPEQLRNERQTRAVDLFSLGCVLFFC 750

Query: 791 ITGGRHPFGDRLERDVNIVKNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPL 850
           +TGG+HP+GD  ERDVN++ ++ +L L+ ++PEAV L++ LLNP+P LRP+A +V+ HPL
Sbjct: 751 MTGGKHPYGDNYERDVNVLNDQKDLFLIESLPEAVHLLTGLLNPDPNLRPRAQDVMHHPL 810

Query: 851 FWSSEMRLSFLRDTSDRIELEDRE--TNLLKALESTAPLALGTEWDKKLERIFITNIGRY 910
           FW+S+MRLSFLRD SDR+ELE+RE  + LL ALESTA + L   WD+KL+ IF+ NIGRY
Sbjct: 811 FWNSDMRLSFLRDASDRVELENREEGSQLLAALESTAAVTLNGRWDEKLDSIFLDNIGRY 866

Query: 911 RRYKYDSVRDLLRVMRNKLNHYRELPKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVIS 967
           RRYK+DS+RDLLRV+RNKLNHYRELPKE+QEL+GS+PEGF+ YF++RFP+LLI+V+ V+ 
Sbjct: 871 RRYKFDSIRDLLRVIRNKLNHYRELPKELQELLGSVPEGFERYFSSRFPKLLIQVYTVLF 866

BLAST of HG10016295 vs. TAIR 10
Match: AT3G11870.1 (Endoribonuclease/protein kinase IRE1-like )

HSP 1 Score: 303.1 bits (775), Expect = 7.8e-82
Identity = 232/611 (37.97%), Postives = 320/611 (52.37%), Query Frame = 0

Query: 389 MGTLAGVVGLFFVFL----ITMLVGLMKYGRTLAVRVKQSLLKEKSFSTSNSRVNSSKKN 448
           +G L  VV +F  F     +  +V   K  +  A RV  S        T N +V   KK+
Sbjct: 9   VGLLVVVVCVFLRFSKDKGLDGIVNEKKRDKNSAPRVSAS----GEDGTKNEQV--EKKS 68

Query: 449 KPR----KSKRSENSEKRDVSISSE--IEDTLMQSEGSLYNGFHDNNLTNIAGSGRRIGK 508
            P     +     NSE + +S+ S+  I  TL     SL    +D N T      RR   
Sbjct: 69  DPSGGLGEENEKTNSESKVLSVPSDQNINKTLPVMLPSLELRKYDENETPGKVVNRR--- 128

Query: 509 LFVTNKKIATGSNGTIILEGIYEGR-PVAVKRLVKTHHDVA--SKEVQNLIVSDRHPNIV 568
           L V+  ++  G NG  + +G+Y  R  VAVK L   H   A    E+ N  + D H NI+
Sbjct: 129 LLVSTNEMKYGRNGYEVFQGVYGRRSSVAVKCLDLAHTTEAFIQNEIDNHCLCDDHSNII 188

Query: 569 RWYGMDSDQDFVYLSLERCTCSLDDLIQIYSHLPMNSMLGLERDTGHMDEYNLHLESIKD 628
           R++G++ DQ F Y+ LE   CSLDDLI++       S+   +RDT    +    ++ ++ 
Sbjct: 189 RFHGLEQDQSFAYICLEPWKCSLDDLIKL-------SVRRTKRDT----QAVAPVDDLEK 248

Query: 629 AMPNLILWKEKGRPSSV--LLKLMRYASEPKIWLVKYRDMVAGLEHLHELGIIHRDLKPQ 688
            M  +  WKEKG+P  +  +LKLM             RD+V GL HLH+L  IHR+L PQ
Sbjct: 249 VMKRIKFWKEKGKPLPLTPMLKLM-------------RDVVCGLAHLHKLKTIHRNLNPQ 308

Query: 689 NVL-IVKQQSICSKLSDMGISKRLPANVSSLGHRATGKYPSIAIISQLQYSYYYKWVIRD 748
           NVL IVK  ++ +K+SDM +SK L    SS  H AT                        
Sbjct: 309 NVLIIVKDMTLTAKISDMSLSKHLGGKKSSYKHLATCS---------------------- 368

Query: 749 KNPIVGCGSSGWQAPEQLLRGRQTR---AIDLFSLGCVLFFCITGGRHPFGDRLERDVNI 808
                  GSSGWQAPEQL + ++ +     D+F+ GC+L + +  G HPFG   ERD NI
Sbjct: 369 -------GSSGWQAPEQLNKDKKKKEDFPADMFNFGCLLHYAVM-GTHPFGSPSERDTNI 428

Query: 809 -VKNKMNLLLVNNIPEAVDLISRLLNPNPGLRPKASEVLQHPLFWSSEMRLSFLRDTSDR 868
              NK NL LV N+ EA++LI +LLN  P LRP A++VL HPLFW SE RL FLR+ SDR
Sbjct: 429 KTNNKTNLSLVTNL-EAINLIEQLLNYKPDLRPSATQVLLHPLFWDSEKRLFFLREASDR 488

Query: 869 IELEDRETNLLKALESTAPLALG--TEWDKKLERIFITNIGRY-------------RRYK 928
           IEL+      L   ++ AP  LG   +W  KL + FIT+I                R YK
Sbjct: 489 IELDITMWGDLN--KTIAPRVLGESKDWASKLGKTFITHIENLAQAQPGQESRQYNRSYK 548

Query: 929 YDSVRDLLRVMRNKLNHYREL--PKEIQELIGSIPEGFDDYFTTRFPRLLIEVHKVISRF 963
           Y S+R LLR++RN L+H+RE+    +I+E++G +PEG D +FT RFP L++E++  IS  
Sbjct: 549 YWSLRHLLRLIRNILSHHREILDDPKIKEMVGKVPEGLDIFFTARFPNLMMEIYAFISMH 553