HG10016278 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10016278 vs. NCBI nr
Match: XP_038881533.1 (E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida] >XP_038881535.1 E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida])

HSP 1 Score: 772.3 bits (1993), Expect = 2.1e-219
Identity = 372/424 (87.74%), Postives = 395/424 (93.16%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPSTSSGPSFRYPSHKINGDDFESLQQV 60
           MG + SKHIH H+HIHG NQEQPSKCSCGH+  PSTSSGPS +YPS+KINGDDFESL+QV
Sbjct: 1   MGGRPSKHIHTHVHIHGLNQEQPSKCSCGHSPPPSTSSGPSSQYPSYKINGDDFESLEQV 60

Query: 61  TEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLE 120
           T+AL +AGLESSNLI+GIDFTKSNEWTGTKSFMG+NLHDLAGAFLNPYEQAISIIGRTLE
Sbjct: 61  TDALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGDREVFSFHP+DRPC+GF+EVL CYRE VPHVKLAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPDDRPCHGFEEVLFCYRERVPHVKLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDT   HLSPQEQSTIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTRPGHLSPQEQSTIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQ 300
           LVGVGDGPW QMN+ DDQI ARDFDNFQFVNFTEIMSKGT P KKE EFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWGQMNECDDQIPARDFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDITT-SASGCSTPS 360
           YKET+RLQLLGQKRETQEH PFPLPRPPPF N FRNYSP+YRRTDSDIT+ S SG S+P+
Sbjct: 301 YKETMRLQLLGQKRETQEHLPFPLPRPPPFINNFRNYSPSYRRTDSDITSASFSGPSSPA 360

Query: 361 HSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSI 420
           HS+L+RSSTWSSS SFN+ICPKCY NEKDLAFGCGHQTCSDCGKDL+FCPTCQTQIT SI
Sbjct: 361 HSILSRSSTWSSSPSFNRICPKCYFNEKDLAFGCGHQTCSDCGKDLIFCPTCQTQITKSI 420

Query: 421 KLRN 424
           KLRN
Sbjct: 421 KLRN 424

BLAST of HG10016278 vs. NCBI nr
Match: XP_008440952.1 (PREDICTED: E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo] >KAA0025590.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa] >TYK12465.1 E3 ubiquitin-protein ligase RGLG2-like [Cucumis melo var. makuwa])

HSP 1 Score: 736.9 bits (1901), Expect = 9.9e-209
Identity = 357/424 (84.20%), Postives = 384/424 (90.57%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPSTSSGPSFRYPSHKINGDDFESLQQV 60
           MG K SKHIH HLHIH   + QPSKCSCGH   PSTSS PSF+YP HKINGDDFESL+QV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLE 120
           TEAL +AGLESSNLI+GIDFTKSNEWTGTKSFMG+NLHDLAGAFLNPYEQAISIIGRTLE
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAP 180
           KFDDDN IPCYGFGD TTGDREVFSFH  DRPC+GF+EVL CYRE VP V LAGPTSFAP
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR+IDT + H SPQEQ+TIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQ 300
           LVGVGDGPWDQMN+ DD+I AR FDNFQFVNFTEIMSKGT P KKE EFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRN-YSPNYRRTDSDITTSASGCSTPS 360
           YKET+RLQLLGQK+ETQ+H PFPLPRPPP TNI RN Y P+YRRTDSDI++S SG ++P+
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYPPSYRRTDSDISSSFSGSTSPA 360

Query: 361 HSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSI 420
           HSVL+RSSTWSS+ SFN+ICPKCYLNEK+LAFGCGHQTCSDCGKDL+FCPTCQTQIT SI
Sbjct: 361 HSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCSDCGKDLLFCPTCQTQITKSI 420

Query: 421 KLRN 424
           KLRN
Sbjct: 421 KLRN 424

BLAST of HG10016278 vs. NCBI nr
Match: XP_031744135.1 (E3 ubiquitin-protein ligase RGLG2-like isoform X1 [Cucumis sativus] >KGN49018.1 hypothetical protein Csa_004004 [Cucumis sativus])

HSP 1 Score: 710.7 bits (1833), Expect = 7.6e-201
Identity = 351/440 (79.77%), Postives = 382/440 (86.82%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPST----------------SSGPSFRY 60
           MG + S+ I N +HI+    +QPSKCSCG    PST                SSGPSF+ 
Sbjct: 1   MGGQPSRQICN-IHIYDTTPKQPSKCSCGRTLPPSTSHTLPPSTSRTLPPNMSSGPSFQN 60

Query: 61  PSHKINGDDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAF 120
           PSHKINGDDFESL+QVTEAL++AGLESSNLI+GIDFT SNEWTGTKSFMG+NLHDLAGAF
Sbjct: 61  PSHKINGDDFESLEQVTEALVKAGLESSNLIIGIDFTISNEWTGTKSFMGRNLHDLAGAF 120

Query: 121 LNPYEQAISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYR 180
           LNPYEQAISIIGRTLEKFDDDNIIPCYGFGD  TGDREVFSFH  DRPC+GF+EVL CYR
Sbjct: 121 LNPYEQAISIIGRTLEKFDDDNIIPCYGFGDVATGDREVFSFHSGDRPCHGFEEVLFCYR 180

Query: 181 ETVPHVKLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQS 240
           E VPHV+LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRS+DT + HLSPQEQ+
Sbjct: 181 EIVPHVRLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSVDTRKGHLSPQEQA 240

Query: 241 TIDAIVEASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFK 300
           TIDAIVEASKYPLSIILVGVGDGPWDQM++ DD++  R FDNFQFVNFTEIMSKGT P K
Sbjct: 241 TIDAIVEASKYPLSIILVGVGDGPWDQMSECDDKVPGRQFDNFQFVNFTEIMSKGTSPCK 300

Query: 301 KEAEFALQCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFR-NYSPNYRR 360
           KE EFALQCLMEIPPQYKET RLQLLGQKRETQEH PFPLPRPPP TNI R +Y+P YRR
Sbjct: 301 KETEFALQCLMEIPPQYKETTRLQLLGQKRETQEHLPFPLPRPPPITNIPRYHYTPIYRR 360

Query: 361 TDSDITTSASGCSTPSHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGK 420
           TDSDIT+S SG ++P+HSVL+RSSTWSSS SF +ICPKCY NEK+LAFGCGHQTCSDCGK
Sbjct: 361 TDSDITSSFSGSTSPAHSVLSRSSTWSSSMSFYRICPKCYTNEKELAFGCGHQTCSDCGK 420

Query: 421 DLVFCPTCQTQITNSIKLRN 424
           DL+FCPTCQTQIT SIKLRN
Sbjct: 421 DLLFCPTCQTQITKSIKLRN 439

BLAST of HG10016278 vs. NCBI nr
Match: XP_031744136.1 (E3 ubiquitin-protein ligase RGLG2-like isoform X2 [Cucumis sativus])

HSP 1 Score: 679.5 bits (1752), Expect = 1.9e-191
Identity = 339/440 (77.05%), Postives = 368/440 (83.64%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPST----------------SSGPSFRY 60
           MG + S+ I N +HI+    +QPSKCSCG    PST                SSGPSF+ 
Sbjct: 1   MGGQPSRQICN-IHIYDTTPKQPSKCSCGRTLPPSTSHTLPPSTSRTLPPNMSSGPSFQN 60

Query: 61  PSHKINGDDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAF 120
           PSHKINGDDFESL+QVTEAL++AGLESSNLI+GIDFT SNEWTGTKSFMG+NLHDLAGAF
Sbjct: 61  PSHKINGDDFESLEQVTEALVKAGLESSNLIIGIDFTISNEWTGTKSFMGRNLHDLAGAF 120

Query: 121 LNPYEQAISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYR 180
           LNPYEQAISIIGRTLEKFDDDNIIPCYGFGD  TGDREVFSFH  DRPC+GF+EVL CYR
Sbjct: 121 LNPYEQAISIIGRTLEKFDDDNIIPCYGFGDVATGDREVFSFHSGDRPCHGFEEVLFCYR 180

Query: 181 ETVPHVKLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQS 240
           E VPHV+LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRS+DT + HLSPQEQ+
Sbjct: 181 EIVPHVRLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSVDTRKGHLSPQEQA 240

Query: 241 TIDAIVEASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFK 300
           TIDAIVEASKYPLSIILVGVGDGPWDQM++ DD++  R FDNFQFVNFTEIMSKGT P K
Sbjct: 241 TIDAIVEASKYPLSIILVGVGDGPWDQMSECDDKVPGRQFDNFQFVNFTEIMSKGTSPCK 300

Query: 301 KEAEFALQCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFR-NYSPNYRR 360
           KE EFALQCLMEIPPQYKET RLQLLGQKRETQEH PFPLPRPPP TNI R +Y+P YRR
Sbjct: 301 KETEFALQCLMEIPPQYKETTRLQLLGQKRETQEHLPFPLPRPPPITNIPRYHYTPIYRR 360

Query: 361 TDSDITTSASGCSTPSHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGK 420
           TDSDIT+S SG ++P+HS                ICPKCY NEK+LAFGCGHQTCSDCGK
Sbjct: 361 TDSDITSSFSGSTSPAHS----------------ICPKCYTNEKELAFGCGHQTCSDCGK 420

Query: 421 DLVFCPTCQTQITNSIKLRN 424
           DL+FCPTCQTQIT SIKLRN
Sbjct: 421 DLLFCPTCQTQITKSIKLRN 423

BLAST of HG10016278 vs. NCBI nr
Match: XP_023544775.1 (E3 ubiquitin-protein ligase RGLG5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 674.1 bits (1738), Expect = 7.9e-190
Identity = 335/435 (77.01%), Postives = 373/435 (85.75%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGH-----------NHSPSTSSGPSFRYPSHKI 60
           MG KSSK+IH H+H++G ++ QP +CSCG+           + SPST+S PS R P+  I
Sbjct: 1   MGAKSSKNIHCHVHLYGDHRNQPLRCSCGYSPPPTTSPWPSSPSPSTNSAPSSRPPNPII 60

Query: 61  NGDDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYE 120
           NGDDFESL+QV+EAL +AGLESSNLIVGIDFTKSNEWTG KSFMG+NLHDL GA LNPYE
Sbjct: 61  NGDDFESLEQVSEALEKAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYE 120

Query: 121 QAISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPH 180
           QAISIIG+TLEKFDDDNIIPCYGFGD TTGDR+VFSFHP D PC+GF+EVL CYRETVPH
Sbjct: 121 QAISIIGKTLEKFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPH 180

Query: 181 VKLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAI 240
           VKLAGPTSFAPIIRNAIRIVNDS GQYHILLIIADGQVTRS+DT + HLSPQEQSTI+AI
Sbjct: 181 VKLAGPTSFAPIIRNAIRIVNDSDGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAI 240

Query: 241 VEASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEF 300
           VEASKYPLSIILVGVGDGPWDQM + DDQI ARDFDNFQFVNFT+IMSKGTPP KK+ EF
Sbjct: 241 VEASKYPLSIILVGVGDGPWDQMYRCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEF 300

Query: 301 ALQCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDI- 360
           ALQCLMEIPPQYK T+RLQLLGQKR TQ H PFPLPRPPP T I RN+ PN+RRT+SDI 
Sbjct: 301 ALQCLMEIPPQYKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDIS 360

Query: 361 TTSASGCSTPSHSVLTRSSTWSS-STSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVF 420
           +TS  G S+PSHS L RSS+ SS S+SFN+IC KCY+NEKDLAFGCGHQ CS CG+DL+ 
Sbjct: 361 STSFYGSSSPSHSALLRSSSVSSPSSSFNRICSKCYINEKDLAFGCGHQICSVCGEDLIL 420

Query: 421 CPTCQTQITNSIKLR 423
           CP CQT IT  I+LR
Sbjct: 421 CPICQTHITTRIRLR 435

BLAST of HG10016278 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.6e-121
Identity = 241/415 (58.07%), Postives = 281/415 (67.71%), Query Frame = 0

Query: 20  QEQPSKCSCGHNHSPSTSSGPSFRYPS---------HKINGDDFESLQQVTEALIQAGLE 79
           Q  P   S     +PS S+ PS  Y S         +    DD+ SL+QVTEAL +AGLE
Sbjct: 60  QPPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLE 119

Query: 80  SSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLEKFDDDNIIPC 139
           SSNLIVGIDFTKSNEWTG +SF  K+LH   G+  NPYEQAI+IIGRTL  FD+DN+IPC
Sbjct: 120 SSNLIVGIDFTKSNEWTGARSFNRKSLH-FIGSSPNPYEQAITIIGRTLAAFDEDNLIPC 179

Query: 140 YGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAPIIRNAIRIVN 199
           YGFGDA+T D++VFSF+ EDR CNGF+EVL  Y+E VP +KLAGPTSFAPII  A+ IV 
Sbjct: 180 YGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVE 239

Query: 200 DSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSIILVGVGDGPWD 259
            SGGQYH+L+IIADGQVTRS+DT    LSPQEQ T+DAIV+ASK PLSI+LVGVGDGPWD
Sbjct: 240 QSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWD 299

Query: 260 QMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQYKETIRLQLL 319
            M ++DD I AR FDNFQFVNFTEIM+K      KE EFAL  LMEIP QYK TI L LL
Sbjct: 300 MMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLL 359

Query: 320 GQKRETQEHFPFPLPRPPPFTNIFRNY-SPNYRRTDSDITTSASGCSTPSHSVLTRSSTW 379
           G++     + P   P PPP      +Y SP   R  S      S    P+     RSS  
Sbjct: 360 GRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLPS---FKPSVPPHPTEGYHVRSSPV 419

Query: 380 ---SSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSIKL 422
              +SS S N++CP C  N KD+AFGCGHQTC +CG DL  CP C+  I   IKL
Sbjct: 420 PPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of HG10016278 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 9.9e-119
Identity = 218/371 (58.76%), Postives = 266/371 (71.70%), Query Frame = 0

Query: 52  DDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQA 111
           D++ S+ +VT AL  AGLESSNLIVGID TKSNEWTG +SF  K+LH   G   NPY+QA
Sbjct: 72  DNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKSLH-FIGTTPNPYQQA 131

Query: 112 ISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVK 171
           ISIIG+TL  FD+DN+IPCYGFGDATT D++VFSF+P D  CNGF+EVL CYRE VP ++
Sbjct: 132 ISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFEEVLMCYREIVPQLR 191

Query: 172 LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVE 231
           L+GPTSFAPII  A+ IV +SGGQYH+LLIIADGQVTRS+DT     SPQEQ TIDAIV 
Sbjct: 192 LSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGGFSPQEQQTIDAIVR 251

Query: 232 ASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFAL 291
           AS+YPLSI+LVGVGDGPWD M ++DD I AR FDNFQFVNFT+IMSK   P +KEAEFAL
Sbjct: 252 ASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMSKNIDPARKEAEFAL 311

Query: 292 QCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDITTS 351
             LMEIP QYK T+ L LLGQ+     H P  +  PPP       Y+    R     + S
Sbjct: 312 SALMEIPSQYKATLELGLLGQR---TGHCPDRIALPPP------TYATQSMRNSPRTSRS 371

Query: 352 ASGCSTP-SHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPT 411
            S  + P  + V +   + + + S  + CP C ++ K++AF CGHQTC+ CG+DL  CP 
Sbjct: 372 TSFQNKPYDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAGCGEDLHVCPI 431

Query: 412 CQTQITNSIKL 422
           C++ I+  IKL
Sbjct: 432 CRSSISVRIKL 432

BLAST of HG10016278 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.4e-117
Identity = 231/411 (56.20%), Postives = 275/411 (66.91%), Query Frame = 0

Query: 16  HGANQEQPSKCSCGHNHSPSTSSG-----PSFRYPSHKINGDDFESLQQVTEALIQAGLE 75
           H  +QE  +  +   N+ P  S         F     KI+ D++ SL QV+EAL +AGLE
Sbjct: 95  HQYSQEGYASAASQPNYPPPPSQSQVADRKKFDRRYSKIS-DNYSSLLQVSEALGRAGLE 154

Query: 76  SSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLEKFDDDNIIPC 135
           SSNLIVGIDFTKSNEWTG KSF  K+LH L+    NPYEQAI+IIGRTL  FD+DN+IPC
Sbjct: 155 SSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNT-PNPYEQAITIIGRTLAAFDEDNLIPC 214

Query: 136 YGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAPIIRNAIRIVN 195
           YGFGDA+T D++VFSF+PE R CNGF+EVL  YRE VP +KLAGPTSFAPII  A+ +V 
Sbjct: 215 YGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPIIEMAMTVVE 274

Query: 196 DSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSIILVGVGDGPWD 255
            S GQYH+L+IIADGQVTRS+DT    LSPQEQ T+DAIV+AS  PLSI+LVGVGDGPWD
Sbjct: 275 QSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLVGVGDGPWD 334

Query: 256 QMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQYKETIRLQLL 315
            M ++DD I AR FDNFQFVNFTEIMSK     +KE EFAL  LMEIPPQYK TI L LL
Sbjct: 335 MMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYKATIELNLL 394

Query: 316 GQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDITTSASGCSTPSHSVLTRSSTWS 375
           G +     + P  +P PPP             ++ S  ++S      PS       S   
Sbjct: 395 GVR---NGNIPQRIPLPPPV------------QSGSSFSSSRIPNFEPSVPPYPFESKQM 454

Query: 376 SSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSIKL 422
           SS    ++CP C  N K++AFGCGHQTC +CG DL  CP C+  I   IKL
Sbjct: 455 SSADDIQLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKL 488

BLAST of HG10016278 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 2.7e-108
Identity = 198/370 (53.51%), Postives = 257/370 (69.46%), Query Frame = 0

Query: 52  DDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQA 111
           D F SL QV++AL +AGLESSNLI+G+DFTKSNEWTG  SF GK LH L G   NPYE+A
Sbjct: 58  DRFSSLDQVSKALREAGLESSNLILGVDFTKSNEWTGKTSFDGKCLHAL-GETSNPYEKA 117

Query: 112 ISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVK 171
           I +IG+TL  FD+DN+IPC+GFGD+TT D EVF FH ++ PC+GF+EVL CY+   P+++
Sbjct: 118 IFVIGQTLAPFDEDNLIPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKRIAPNLR 177

Query: 172 LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVE 231
           L+GPTS+ P+I  A+ IV  + GQ+H+L+I+ADGQVTR  D  E  LS QE++TIDAIV 
Sbjct: 178 LSGPTSYGPLIDAAVDIVEKNNGQFHVLVIVADGQVTRGTDMAEGELSQQEKTTIDAIVN 237

Query: 232 ASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFAL 291
           AS Y LSI+LVGVGDGPW+ M K+DD+I  R+FDNFQFVNFTEIM++ +P   KE  FAL
Sbjct: 238 ASSYALSIVLVGVGDGPWEDMRKFDDKIPKREFDNFQFVNFTEIMTRNSPESAKETAFAL 297

Query: 292 QCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDITTS 351
             LMEIP QY+  I L+LLG++    +     +PRPPP       Y+P    T++++ ++
Sbjct: 298 AALMEIPFQYQAAIELRLLGKQTGLAKTI---VPRPPPIP-----YTP---PTNAELPST 357

Query: 352 ASGCSTPSHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTC 411
           AS    P+    T+S            CP C  N KD+AF CGH TC DCG  +  CP C
Sbjct: 358 AS----PASPEQTQS------------CPICLTNRKDVAFSCGHMTCGDCGSKISNCPIC 399

Query: 412 QTQITNSIKL 422
           + +ITN +KL
Sbjct: 418 RVRITNRLKL 399

BLAST of HG10016278 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 6.5e-102
Identity = 195/370 (52.70%), Postives = 243/370 (65.68%), Query Frame = 0

Query: 52  DDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQA 111
           D F SL QV  +L +AGLESSNLI+GIDFTKSNEWTG  SF  K+LH + G   NPYE+A
Sbjct: 16  DHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAI-GKRQNPYEKA 75

Query: 112 ISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVK 171
           ISIIGRTL  FD+D++IPC+GFGD TT D+ VFSF+PE++ C+G +  +  YRE VPH+K
Sbjct: 76  ISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVPHLK 135

Query: 172 LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVE 231
           L+GPTSFAP+I  AI IV  +  QYH+L+IIADGQVTR+ D     LSPQE++T+++I+ 
Sbjct: 136 LSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNSIMA 195

Query: 232 ASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFAL 291
           AS YPLSI+LVGVGDGPWD M ++DD I  R+FDNFQFVNFT+IMS+     KKEA FAL
Sbjct: 196 ASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDAAKKEAAFAL 255

Query: 292 QCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDITTS 351
             LMEIP QYK T+ L     +   Q H P P   PPP                  I   
Sbjct: 256 AALMEIPFQYKATLSLNRKPVRSSHQHHKPLP---PPP----------------EVIERD 315

Query: 352 ASGCSTPSHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTC 411
            +  S P+    T   +   + S   +CP C  N KD+AF CGH TC +CG  +  CP C
Sbjct: 316 NAVRSVPNQMTETAEKSDRLAPSTVPVCPICLTNPKDMAFSCGHTTCKECGVVITTCPLC 365

Query: 412 QTQITNSIKL 422
           +  IT  I+L
Sbjct: 376 RQPITTRIRL 365

BLAST of HG10016278 vs. ExPASy TrEMBL
Match: A0A5D3CLM2 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G00410 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 4.8e-209
Identity = 357/424 (84.20%), Postives = 384/424 (90.57%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPSTSSGPSFRYPSHKINGDDFESLQQV 60
           MG K SKHIH HLHIH   + QPSKCSCGH   PSTSS PSF+YP HKINGDDFESL+QV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLE 120
           TEAL +AGLESSNLI+GIDFTKSNEWTGTKSFMG+NLHDLAGAFLNPYEQAISIIGRTLE
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAP 180
           KFDDDN IPCYGFGD TTGDREVFSFH  DRPC+GF+EVL CYRE VP V LAGPTSFAP
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR+IDT + H SPQEQ+TIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQ 300
           LVGVGDGPWDQMN+ DD+I AR FDNFQFVNFTEIMSKGT P KKE EFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRN-YSPNYRRTDSDITTSASGCSTPS 360
           YKET+RLQLLGQK+ETQ+H PFPLPRPPP TNI RN Y P+YRRTDSDI++S SG ++P+
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYPPSYRRTDSDISSSFSGSTSPA 360

Query: 361 HSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSI 420
           HSVL+RSSTWSS+ SFN+ICPKCYLNEK+LAFGCGHQTCSDCGKDL+FCPTCQTQIT SI
Sbjct: 361 HSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCSDCGKDLLFCPTCQTQITKSI 420

Query: 421 KLRN 424
           KLRN
Sbjct: 421 KLRN 424

BLAST of HG10016278 vs. ExPASy TrEMBL
Match: A0A1S3B2Y1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo OX=3656 GN=LOC103485213 PE=4 SV=1)

HSP 1 Score: 736.9 bits (1901), Expect = 4.8e-209
Identity = 357/424 (84.20%), Postives = 384/424 (90.57%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPSTSSGPSFRYPSHKINGDDFESLQQV 60
           MG K SKHIH HLHIH   + QPSKCSCGH   PSTSS PSF+YP HKINGDDFESL+QV
Sbjct: 1   MGGKPSKHIHTHLHIHDNTRVQPSKCSCGHTLPPSTSSRPSFQYPRHKINGDDFESLEQV 60

Query: 61  TEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLE 120
           TEAL +AGLESSNLI+GIDFTKSNEWTGTKSFMG+NLHDLAGAFLNPYEQAISIIGRTLE
Sbjct: 61  TEALEKAGLESSNLIIGIDFTKSNEWTGTKSFMGRNLHDLAGAFLNPYEQAISIIGRTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAP 180
           KFDDDN IPCYGFGD TTGDREVFSFH  DRPC+GF+EVL CYRE VP V LAGPTSFAP
Sbjct: 121 KFDDDNKIPCYGFGDVTTGDREVFSFHSGDRPCHGFEEVLFCYREIVPRVLLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTR+IDT + H SPQEQ+TIDAIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSCGQYHILLIIADGQVTRNIDTRQGHFSPQEQATIDAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQ 300
           LVGVGDGPWDQMN+ DD+I AR FDNFQFVNFTEIMSKGT P KKE EFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMNECDDKIPARQFDNFQFVNFTEIMSKGTSPSKKETEFALQCLMEIPPQ 300

Query: 301 YKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRN-YSPNYRRTDSDITTSASGCSTPS 360
           YKET+RLQLLGQK+ETQ+H PFPLPRPPP TNI RN Y P+YRRTDSDI++S SG ++P+
Sbjct: 301 YKETMRLQLLGQKKETQQHLPFPLPRPPPITNIRRNYYPPSYRRTDSDISSSFSGSTSPA 360

Query: 361 HSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSI 420
           HSVL+RSSTWSS+ SFN+ICPKCYLNEK+LAFGCGHQTCSDCGKDL+FCPTCQTQIT SI
Sbjct: 361 HSVLSRSSTWSSTPSFNRICPKCYLNEKELAFGCGHQTCSDCGKDLLFCPTCQTQITKSI 420

Query: 421 KLRN 424
           KLRN
Sbjct: 421 KLRN 424

BLAST of HG10016278 vs. ExPASy TrEMBL
Match: A0A0A0KHN3 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G510360 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.7e-201
Identity = 351/440 (79.77%), Postives = 382/440 (86.82%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPST----------------SSGPSFRY 60
           MG + S+ I N +HI+    +QPSKCSCG    PST                SSGPSF+ 
Sbjct: 1   MGGQPSRQICN-IHIYDTTPKQPSKCSCGRTLPPSTSHTLPPSTSRTLPPNMSSGPSFQN 60

Query: 61  PSHKINGDDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAF 120
           PSHKINGDDFESL+QVTEAL++AGLESSNLI+GIDFT SNEWTGTKSFMG+NLHDLAGAF
Sbjct: 61  PSHKINGDDFESLEQVTEALVKAGLESSNLIIGIDFTISNEWTGTKSFMGRNLHDLAGAF 120

Query: 121 LNPYEQAISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYR 180
           LNPYEQAISIIGRTLEKFDDDNIIPCYGFGD  TGDREVFSFH  DRPC+GF+EVL CYR
Sbjct: 121 LNPYEQAISIIGRTLEKFDDDNIIPCYGFGDVATGDREVFSFHSGDRPCHGFEEVLFCYR 180

Query: 181 ETVPHVKLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQS 240
           E VPHV+LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRS+DT + HLSPQEQ+
Sbjct: 181 EIVPHVRLAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSVDTRKGHLSPQEQA 240

Query: 241 TIDAIVEASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFK 300
           TIDAIVEASKYPLSIILVGVGDGPWDQM++ DD++  R FDNFQFVNFTEIMSKGT P K
Sbjct: 241 TIDAIVEASKYPLSIILVGVGDGPWDQMSECDDKVPGRQFDNFQFVNFTEIMSKGTSPCK 300

Query: 301 KEAEFALQCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFR-NYSPNYRR 360
           KE EFALQCLMEIPPQYKET RLQLLGQKRETQEH PFPLPRPPP TNI R +Y+P YRR
Sbjct: 301 KETEFALQCLMEIPPQYKETTRLQLLGQKRETQEHLPFPLPRPPPITNIPRYHYTPIYRR 360

Query: 361 TDSDITTSASGCSTPSHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGK 420
           TDSDIT+S SG ++P+HSVL+RSSTWSSS SF +ICPKCY NEK+LAFGCGHQTCSDCGK
Sbjct: 361 TDSDITSSFSGSTSPAHSVLSRSSTWSSSMSFYRICPKCYTNEKELAFGCGHQTCSDCGK 420

Query: 421 DLVFCPTCQTQITNSIKLRN 424
           DL+FCPTCQTQIT SIKLRN
Sbjct: 421 DLLFCPTCQTQITKSIKLRN 439

BLAST of HG10016278 vs. ExPASy TrEMBL
Match: A0A6J1INP4 (E3 ubiquitin-protein ligase RGLG5-like OS=Cucurbita maxima OX=3661 GN=LOC111478078 PE=4 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.5e-189
Identity = 332/424 (78.30%), Postives = 367/424 (86.56%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPSTSSGPSFRYPSHKINGDDFESLQQV 60
           MG KSSK+IH H+H +G ++ QP +CSCGH+  PST+S PS R PS  INGDDFESL+QV
Sbjct: 1   MGAKSSKNIHCHVHFYGDHRNQPLRCSCGHSLPPSTNSAPSSRPPSPIINGDDFESLEQV 60

Query: 61  TEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLE 120
           +EAL  AGLESSNLIVGIDFTKSNEWTG KSFMG+NLHDL GA LNPYEQAISIIG+TLE
Sbjct: 61  SEALENAGLESSNLIVGIDFTKSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGDREVFSFHP D PC+GF+EVL CYRETVPHV LAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDREVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT + HLS QEQSTI+AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSSQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQ 300
           LVGVGDGPWDQM + DDQI AR+FDNFQFVNFT+IMSKGTPP KK+ EFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARNFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDI-TTSASGCSTPS 360
           YK T+RLQLLGQKR TQ H PFPLPRPPP T I RN+ PN+RRT+SDI +TS  G S+PS
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDISSTSFYGSSSPS 360

Query: 361 HSVLTRSSTWSS-STSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNS 420
           HS L RSS+ SS S+SFN+IC +CY+NEKDLA GCGHQ CS CG+DL+ CP CQT IT  
Sbjct: 361 HSALLRSSSVSSPSSSFNRICSQCYINEKDLALGCGHQICSACGEDLIACPICQTHITIR 420

Query: 421 IKLR 423
           I+LR
Sbjct: 421 IRLR 424

BLAST of HG10016278 vs. ExPASy TrEMBL
Match: A0A6J1GF30 (E3 ubiquitin-protein ligase RGLG5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453613 PE=4 SV=1)

HSP 1 Score: 669.1 bits (1725), Expect = 1.2e-188
Identity = 330/424 (77.83%), Postives = 368/424 (86.79%), Query Frame = 0

Query: 1   MGVKSSKHIHNHLHIHGANQEQPSKCSCGHNHSPSTSSGPSFRYPSHKINGDDFESLQQV 60
           MG KSSK+IH H+HI+G ++ QP +CSCG++  PST+S  S R P+  INGDDFESL+QV
Sbjct: 1   MGAKSSKNIHCHVHIYGDHRNQPLRCSCGYSPPPSTNSARSSRPPNPIINGDDFESLEQV 60

Query: 61  TEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLE 120
           +EAL +AGLESSNLIVGIDFT+SNEWTG KSFMG+NLHDL GA LNPYEQAISIIG+TLE
Sbjct: 61  SEALEKAGLESSNLIVGIDFTRSNEWTGKKSFMGRNLHDLDGACLNPYEQAISIIGKTLE 120

Query: 121 KFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAP 180
           KFDDDNIIPCYGFGD TTGDR+VFSFHP D PC+GF+EVL CYRETVPHV LAGPTSFAP
Sbjct: 121 KFDDDNIIPCYGFGDVTTGDRDVFSFHPGDTPCHGFEEVLHCYRETVPHVNLAGPTSFAP 180

Query: 181 IIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSII 240
           IIRNAIRIVNDS GQYHILLIIADGQVTRS+DT + HLSPQEQSTI+AIVEASKYPLSII
Sbjct: 181 IIRNAIRIVNDSYGQYHILLIIADGQVTRSVDTCQGHLSPQEQSTIEAIVEASKYPLSII 240

Query: 241 LVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQ 300
           LVGVGDGPWDQM + DDQI ARDFDNFQFVNFT+IMSKGTPP KK+ EFALQCLMEIPPQ
Sbjct: 241 LVGVGDGPWDQMYQCDDQIPARDFDNFQFVNFTDIMSKGTPPSKKKTEFALQCLMEIPPQ 300

Query: 301 YKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDI-TTSASGCSTPS 360
           YK T+RLQLLGQKR TQ H PFPLPRPPP T I RN+ PN+RRT+SDI +TS  G S+PS
Sbjct: 301 YKATMRLQLLGQKRNTQGHLPFPLPRPPPITYISRNHFPNFRRTESDISSTSFYGSSSPS 360

Query: 361 HSVLTRSSTWSS-STSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNS 420
           HS L RSS+ SS S SFN+IC +CY+NEKDLA GCGHQ CS CG+DL+ CP CQT IT  
Sbjct: 361 HSALLRSSSVSSPSLSFNRICSQCYINEKDLALGCGHQICSVCGEDLILCPICQTHITTR 420

Query: 421 IKLR 423
           I+LR
Sbjct: 421 IRLR 424

BLAST of HG10016278 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 241/415 (58.07%), Postives = 281/415 (67.71%), Query Frame = 0

Query: 20  QEQPSKCSCGHNHSPSTSSGPSFRYPS---------HKINGDDFESLQQVTEALIQAGLE 79
           Q  P   S     +PS S+ PS  Y S         +    DD+ SL+QVTEAL +AGLE
Sbjct: 60  QPPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLE 119

Query: 80  SSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLEKFDDDNIIPC 139
           SSNLIVGIDFTKSNEWTG +SF  K+LH   G+  NPYEQAI+IIGRTL  FD+DN+IPC
Sbjct: 120 SSNLIVGIDFTKSNEWTGARSFNRKSLH-FIGSSPNPYEQAITIIGRTLAAFDEDNLIPC 179

Query: 140 YGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAPIIRNAIRIVN 199
           YGFGDA+T D++VFSF+ EDR CNGF+EVL  Y+E VP +KLAGPTSFAPII  A+ IV 
Sbjct: 180 YGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVE 239

Query: 200 DSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSIILVGVGDGPWD 259
            SGGQYH+L+IIADGQVTRS+DT    LSPQEQ T+DAIV+ASK PLSI+LVGVGDGPWD
Sbjct: 240 QSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWD 299

Query: 260 QMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQYKETIRLQLL 319
            M ++DD I AR FDNFQFVNFTEIM+K      KE EFAL  LMEIP QYK TI L LL
Sbjct: 300 MMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLL 359

Query: 320 GQKRETQEHFPFPLPRPPPFTNIFRNY-SPNYRRTDSDITTSASGCSTPSHSVLTRSSTW 379
           G++     + P   P PPP      +Y SP   R  S      S    P+     RSS  
Sbjct: 360 GRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLPS---FKPSVPPHPTEGYHVRSSPV 419

Query: 380 ---SSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSIKL 422
              +SS S N++CP C  N KD+AFGCGHQTC +CG DL  CP C+  I   IKL
Sbjct: 420 PPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of HG10016278 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 241/415 (58.07%), Postives = 281/415 (67.71%), Query Frame = 0

Query: 20  QEQPSKCSCGHNHSPSTSSGPSFRYPS---------HKINGDDFESLQQVTEALIQAGLE 79
           Q  P   S     +PS S+ PS  Y S         +    DD+ SL+QVTEAL +AGLE
Sbjct: 60  QPPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLE 119

Query: 80  SSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLEKFDDDNIIPC 139
           SSNLIVGIDFTKSNEWTG +SF  K+LH   G+  NPYEQAI+IIGRTL  FD+DN+IPC
Sbjct: 120 SSNLIVGIDFTKSNEWTGARSFNRKSLH-FIGSSPNPYEQAITIIGRTLAAFDEDNLIPC 179

Query: 140 YGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAPIIRNAIRIVN 199
           YGFGDA+T D++VFSF+ EDR CNGF+EVL  Y+E VP +KLAGPTSFAPII  A+ IV 
Sbjct: 180 YGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVE 239

Query: 200 DSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSIILVGVGDGPWD 259
            SGGQYH+L+IIADGQVTRS+DT    LSPQEQ T+DAIV+ASK PLSI+LVGVGDGPWD
Sbjct: 240 QSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWD 299

Query: 260 QMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQYKETIRLQLL 319
            M ++DD I AR FDNFQFVNFTEIM+K      KE EFAL  LMEIP QYK TI L LL
Sbjct: 300 MMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLL 359

Query: 320 GQKRETQEHFPFPLPRPPPFTNIFRNY-SPNYRRTDSDITTSASGCSTPSHSVLTRSSTW 379
           G++     + P   P PPP      +Y SP   R  S      S    P+     RSS  
Sbjct: 360 GRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLPS---FKPSVPPHPTEGYHVRSSPV 419

Query: 380 ---SSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSIKL 422
              +SS S N++CP C  N KD+AFGCGHQTC +CG DL  CP C+  I   IKL
Sbjct: 420 PPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of HG10016278 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 241/415 (58.07%), Postives = 281/415 (67.71%), Query Frame = 0

Query: 20  QEQPSKCSCGHNHSPSTSSGPSFRYPS---------HKINGDDFESLQQVTEALIQAGLE 79
           Q  P   S     +PS S+ PS  Y S         +    DD+ SL+QVTEAL +AGLE
Sbjct: 60  QPPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLE 119

Query: 80  SSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLEKFDDDNIIPC 139
           SSNLIVGIDFTKSNEWTG +SF  K+LH   G+  NPYEQAI+IIGRTL  FD+DN+IPC
Sbjct: 120 SSNLIVGIDFTKSNEWTGARSFNRKSLH-FIGSSPNPYEQAITIIGRTLAAFDEDNLIPC 179

Query: 140 YGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAPIIRNAIRIVN 199
           YGFGDA+T D++VFSF+ EDR CNGF+EVL  Y+E VP +KLAGPTSFAPII  A+ IV 
Sbjct: 180 YGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVE 239

Query: 200 DSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSIILVGVGDGPWD 259
            SGGQYH+L+IIADGQVTRS+DT    LSPQEQ T+DAIV+ASK PLSI+LVGVGDGPWD
Sbjct: 240 QSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWD 299

Query: 260 QMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQYKETIRLQLL 319
            M ++DD I AR FDNFQFVNFTEIM+K      KE EFAL  LMEIP QYK TI L LL
Sbjct: 300 MMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLL 359

Query: 320 GQKRETQEHFPFPLPRPPPFTNIFRNY-SPNYRRTDSDITTSASGCSTPSHSVLTRSSTW 379
           G++     + P   P PPP      +Y SP   R  S      S    P+     RSS  
Sbjct: 360 GRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLPS---FKPSVPPHPTEGYHVRSSPV 419

Query: 380 ---SSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSIKL 422
              +SS S N++CP C  N KD+AFGCGHQTC +CG DL  CP C+  I   IKL
Sbjct: 420 PPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of HG10016278 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 437.6 bits (1124), Expect = 1.2e-122
Identity = 241/415 (58.07%), Postives = 281/415 (67.71%), Query Frame = 0

Query: 20  QEQPSKCSCGHNHSPSTSSGPSFRYPS---------HKINGDDFESLQQVTEALIQAGLE 79
           Q  P   S     +PS S+ PS  Y S         +    DD+ SL+QVTEAL +AGLE
Sbjct: 60  QPPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLE 119

Query: 80  SSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQAISIIGRTLEKFDDDNIIPC 139
           SSNLIVGIDFTKSNEWTG +SF  K+LH   G+  NPYEQAI+IIGRTL  FD+DN+IPC
Sbjct: 120 SSNLIVGIDFTKSNEWTGARSFNRKSLH-FIGSSPNPYEQAITIIGRTLAAFDEDNLIPC 179

Query: 140 YGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVKLAGPTSFAPIIRNAIRIVN 199
           YGFGDA+T D++VFSF+ EDR CNGF+EVL  Y+E VP +KLAGPTSFAPII  A+ IV 
Sbjct: 180 YGFGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVE 239

Query: 200 DSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVEASKYPLSIILVGVGDGPWD 259
            SGGQYH+L+IIADGQVTRS+DT    LSPQEQ T+DAIV+ASK PLSI+LVGVGDGPWD
Sbjct: 240 QSGGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWD 299

Query: 260 QMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFALQCLMEIPPQYKETIRLQLL 319
            M ++DD I AR FDNFQFVNFTEIM+K      KE EFAL  LMEIP QYK TI L LL
Sbjct: 300 MMREFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLL 359

Query: 320 GQKRETQEHFPFPLPRPPPFTNIFRNY-SPNYRRTDSDITTSASGCSTPSHSVLTRSSTW 379
           G++     + P   P PPP      +Y SP   R  S      S    P+     RSS  
Sbjct: 360 GRR---NGYIPERFPLPPPMRGGSSSYNSPKPSRLPS---FKPSVPPHPTEGYHVRSSPV 419

Query: 380 ---SSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPTCQTQITNSIKL 422
              +SS S N++CP C  N KD+AFGCGHQTC +CG DL  CP C+  I   IKL
Sbjct: 420 PPPTSSASDNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKL 467

BLAST of HG10016278 vs. TAIR 10
Match: AT1G67800.1 (Copine (Calcium-dependent phospholipid-binding protein) family )

HSP 1 Score: 428.3 bits (1100), Expect = 7.0e-120
Identity = 218/371 (58.76%), Postives = 266/371 (71.70%), Query Frame = 0

Query: 52  DDFESLQQVTEALIQAGLESSNLIVGIDFTKSNEWTGTKSFMGKNLHDLAGAFLNPYEQA 111
           D++ S+ +VT AL  AGLESSNLIVGID TKSNEWTG +SF  K+LH   G   NPY+QA
Sbjct: 72  DNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARSFGRKSLH-FIGTTPNPYQQA 131

Query: 112 ISIIGRTLEKFDDDNIIPCYGFGDATTGDREVFSFHPEDRPCNGFQEVLCCYRETVPHVK 171
           ISIIG+TL  FD+DN+IPCYGFGDATT D++VFSF+P D  CNGF+EVL CYRE VP ++
Sbjct: 132 ISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTYCNGFEEVLMCYREIVPQLR 191

Query: 172 LAGPTSFAPIIRNAIRIVNDSGGQYHILLIIADGQVTRSIDTGESHLSPQEQSTIDAIVE 231
           L+GPTSFAPII  A+ IV +SGGQYH+LLIIADGQVTRS+DT     SPQEQ TIDAIV 
Sbjct: 192 LSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVDTDNGGFSPQEQQTIDAIVR 251

Query: 232 ASKYPLSIILVGVGDGPWDQMNKYDDQISARDFDNFQFVNFTEIMSKGTPPFKKEAEFAL 291
           AS+YPLSI+LVGVGDGPWD M ++DD I AR FDNFQFVNFT+IMSK   P +KEAEFAL
Sbjct: 252 ASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNFTDIMSKNIDPARKEAEFAL 311

Query: 292 QCLMEIPPQYKETIRLQLLGQKRETQEHFPFPLPRPPPFTNIFRNYSPNYRRTDSDITTS 351
             LMEIP QYK T+ L LLGQ+     H P  +  PPP       Y+    R     + S
Sbjct: 312 SALMEIPSQYKATLELGLLGQR---TGHCPDRIALPPP------TYATQSMRNSPRTSRS 371

Query: 352 ASGCSTP-SHSVLTRSSTWSSSTSFNKICPKCYLNEKDLAFGCGHQTCSDCGKDLVFCPT 411
            S  + P  + V +   + + + S  + CP C ++ K++AF CGHQTC+ CG+DL  CP 
Sbjct: 372 TSFQNKPYDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGHQTCAGCGEDLHVCPI 431

Query: 412 CQTQITNSIKL 422
           C++ I+  IKL
Sbjct: 432 CRSSISVRIKL 432