HG10016122 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10016122
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionF-box domain-containing protein
LocationChr03: 3001952 .. 3004942 (+)
RNA-Seq ExpressionHG10016122
SyntenyHG10016122
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTTGAAGAGGAAGAAAGGGTCAGGTGATTCGGACGATTTGAATCGGGCCAGTTCAATTTTCCCAGATGAGGTACTGGAAAGAGTTTTGAGTTTGGTGAAGTCTCACAAAGATAGAAGCTCTGTATCTTTGGTTTGTAAAGATTGGTTCAACGCCGAGCGATGGTCTCGAACCCACGTTTTCATCGGGAATTGTTACTCTGTTTCGCCGGAAATTGTAATCCGACGGTTCCCGAACATTCGGAGTGTGACGCTGAAAGGGAAACCTAGGTTTTCAGATTTCAATTTGGTGCCTCCAAATTGGGGAGCTGATATTCATTCATGGCTTGTTGCCTTCGCTTCAAAATACCCAATTCTCGAAGAATTGAGGCTCAAGAGAATGGCTGTAACTGACGAAAGCTTAGAGTTTTTGGGTCGTTCCTTCCCAAATTTCAAAGCCCTTTCAATGATGAGCTGCGATGGCTTCAGCACCGATGGCCTTGCCGCCATTGCCACTCACTGCAAGTACGTCATTTCTTCTTTTAAAAACTCTGATTCCTTACGATGAGATCTGGGTTGTTTGGGGAAATCCCTTTTGAAAAAACAGGGAATTTTATTATATTTATTTATTATTTTCCCTCTCGGAATATTGATTTCTATTGTGTTAGATGCCTCCTCAGAAAAGGGTTTTTTCATTTCCGTTGTGGTTATGAAATTAAAGCAATGTTTGGTTGGTTTGGTCACTGATTCGTTTCTTTCAATTAGCTTTATATGGGGTTGCTGATGGACACCACTCAAACAGTATCATGTCGGAATCATATTGAAAAGTAATGAACTTTATTCATTCATTCATATAAAATGGAATAGAACTCATAATAAATACGATTGTACTGCGTATAATTCTGTTATTGGTGCTTATTATAGCATCACTCCCTACTGATTGAACTCTGATGAGAAATTTTCTAACTTTTGTTATCTCCACCTCTCATTTTTCTGTTTCCCTTTTTTCTTGGATGCGACATCTAACCTGCTTTTTTTTCTGTCATATGGTCGTTGGCATATCACATCCTTGCAACTTTGTCTCCATTATTATCAACTTGATCATTAATTAATTACTAATGATATGGGAAGATCAGTCATTGAACAAATCTGGCCTTCTGCCCATCTCTTTCTACAGGAATCTTCAACCTACTGCAATGGCTCCACTGATTTTGCCAGCTCCCCAAATCTGTTGTCTTTAATGAAGCTTCAGTTTGGGGTGGGGTGGGGGGTTTACTGTGCCTTTTATTTGTTTATTCTTTTATTTTATTTTTTTTTTTTATTATTTTTTTTTTTTTTTCAGGAGGCTACTATTTATTAATACTAATGAATGGCGTTGAGTTTATTATCATAAATTCTAAAATTTATTGCATGTTGTGATGTAGACATAGAATGGATATAAAGGATGACCTTGTCAAAAGTTATCAAAAGTAGAAACCGATTAATTTGTTTCTTTTTACTAGATATGGTCTTGAAAATGATATTTAGTAAAGACATTCAAGCCAAACGTTTTCTTTCCTGGGTGGGTGGTCTGTTTTGGCAAGAAAGCGAAATTCTGACTTCTTAATTTGGTGTCAGGAACTTGACGGAGCTTGACATACTTGAGAATGACATTATTGACAAAAGTGGAAATTGGCTGAGTTGCTTCCCAGATACCTTGAAATCTCTGGAAGTACTCAACTTTTCAAGTTTGAACAGCGATGTCAGTTTTGAGGCCCTTGAGAAGCTGGTAAGAAGGTGCAAATCACTGAAGGTTTTGAAGGTCAATAGGAATATAAACTTGGAACAACTGCAAAGGCTGCTTGTTCACACTCCTCAGTTAACGGAGCTAGGCACGGGCTCTTTTTCGCAAGAGATTACTCTTCGGCAGTACTGCGACCTCGAAGATGCTTTTAAAAGTTGCAAGAATCTGCGTACTCTCTCTGGTTTGTTGGAGTCGACAGTGTTGTATCTCCAAGTTCTTTTTCCTGCCTGTGCAAATTTAACTTTCTTGAATCTGAGCTACGCTATTCTTCATGGTGGTGAACTTGCTGGACTTCTTTCCCACTGCCCTGTTCTTCACCGCCTTTGGGTATGGATTGGTTTTCCCTTCCTTTCTTTAGTTCAATATTAACGAGTGAAAGAGAGGGAGAGCAAGGATTGTGCAATGCAGGCACCTAGGTTCCTTCTATGACTGTTCATACTTGAGCCCTCTAATATTATGTTCCTGGTGCAGGTTTTAGACACAGTTGAAGACAAGGGCCTGAAGGCTGTTGGACTGAGTTGCCCCTTGCTGGAGGAACTCCGTGTATTTCCAGCAGATCCATTTGCTGACAATTTAGTCCATGGTGTAACCGAGTCTGGGTTTCTTGCTGTCTCTTATGGCTGCCGTAAACTCCATTACGTTCTCTACTTTTGCCGTCAGATGACGAATGAGGCTGTGGCCACCATTGTGCAGAACTGCCCAGATTTCACCCATTTTCGTCTCTGCATTATGAACCCCCACCAACCAGACTATCTTACAAAGCAACCAATGGATGAGGCCTTTGGTGCGGTGGTGAAAACTTGCTCTAGGCTGCGCAGACTTGCCATTTCGGGCTTGCTAACGGACTTAACATTTGAATACATTGGGAAATATGCAAAAAACTTGGAAACTCTTTCAGTGGCTTTTGCTGGAAGCAGTGACTGGGGAATGCAGTGTGTTATGAGTGGTTGTCCAAAGCTGAGAAAACTGGAGATCAGAGACAGCCCATTTGGAAATGCAGCTTTGCTTTCTGGTTTGGAGAGGTACGAATCCATGAGATCACTATGGATGTCGGCCTGCAAGGTGACGATGAACGGGTGTCGAGTTTTGGCAAAGCAGGTGCCGAGGTTGAACGTTGAGGTCATTAAGGATGGTGGAAGTGATGAATGCGAGGCTGAAAGTGTTTACGTATACCGCTCTGTTGCAGGTCCACGAAGAGACGCCCCGTCGTTCGTTCTCACCCTCTAA

mRNA sequence

ATGAGTTTGAAGAGGAAGAAAGGGTCAGGTGATTCGGACGATTTGAATCGGGCCAGTTCAATTTTCCCAGATGAGGTACTGGAAAGAGTTTTGAGTTTGGTGAAGTCTCACAAAGATAGAAGCTCTGTATCTTTGGTTTGTAAAGATTGGTTCAACGCCGAGCGATGGTCTCGAACCCACGTTTTCATCGGGAATTGTTACTCTGTTTCGCCGGAAATTGTAATCCGACGGTTCCCGAACATTCGGAGTGTGACGCTGAAAGGGAAACCTAGGTTTTCAGATTTCAATTTGGTGCCTCCAAATTGGGGAGCTGATATTCATTCATGGCTTGTTGCCTTCGCTTCAAAATACCCAATTCTCGAAGAATTGAGGCTCAAGAGAATGGCTGTAACTGACGAAAGCTTAGAGTTTTTGGGTCGTTCCTTCCCAAATTTCAAAGCCCTTTCAATGATGAGCTGCGATGGCTTCAGCACCGATGGCCTTGCCGCCATTGCCACTCACTGCAAGAACTTGACGGAGCTTGACATACTTGAGAATGACATTATTGACAAAAGTGGAAATTGGCTGAGTTGCTTCCCAGATACCTTGAAATCTCTGGAAGTACTCAACTTTTCAAGTTTGAACAGCGATGTCAGTTTTGAGGCCCTTGAGAAGCTGGTAAGAAGGTGCAAATCACTGAAGGTTTTGAAGGTCAATAGGAATATAAACTTGGAACAACTGCAAAGGCTGCTTGTTCACACTCCTCAGTTAACGGAGCTAGGCACGGGCTCTTTTTCGCAAGAGATTACTCTTCGGCAGTACTGCGACCTCGAAGATGCTTTTAAAAGTTGCAAGAATCTGCGTACTCTCTCTGGTTTGTTGGAGTCGACAGTGTTGTATCTCCAAGTTCTTTTTCCTGCCTGTGCAAATTTAACTTTCTTGAATCTGAGCTACGCTATTCTTCATGGTGGTGAACTTGCTGGACTTCTTTCCCACTGCCCTGTTCTTCACCGCCTTTGGGTTTTAGACACAGTTGAAGACAAGGGCCTGAAGGCTGTTGGACTGAGTTGCCCCTTGCTGGAGGAACTCCGTGTATTTCCAGCAGATCCATTTGCTGACAATTTAGTCCATGGTGTAACCGAGTCTGGGTTTCTTGCTGTCTCTTATGGCTGCCGTAAACTCCATTACGTTCTCTACTTTTGCCGTCAGATGACGAATGAGGCTGTGGCCACCATTGTGCAGAACTGCCCAGATTTCACCCATTTTCGTCTCTGCATTATGAACCCCCACCAACCAGACTATCTTACAAAGCAACCAATGGATGAGGCCTTTGGTGCGGTGGTGAAAACTTGCTCTAGGCTGCGCAGACTTGCCATTTCGGGCTTGCTAACGGACTTAACATTTGAATACATTGGGAAATATGCAAAAAACTTGGAAACTCTTTCAGTGGCTTTTGCTGGAAGCAGTGACTGGGGAATGCAGTGTGTTATGAGTGGTTGTCCAAAGCTGAGAAAACTGGAGATCAGAGACAGCCCATTTGGAAATGCAGCTTTGCTTTCTGGTTTGGAGAGGTACGAATCCATGAGATCACTATGGATGTCGGCCTGCAAGGTGACGATGAACGGGTGTCGAGTTTTGGCAAAGCAGGTGCCGAGGTTGAACGTTGAGGTCATTAAGGATGGTGGAAGTGATGAATGCGAGGCTGAAAGTGTTTACGTATACCGCTCTGTTGCAGGTCCACGAAGAGACGCCCCGTCGTTCGTTCTCACCCTCTAA

Coding sequence (CDS)

ATGAGTTTGAAGAGGAAGAAAGGGTCAGGTGATTCGGACGATTTGAATCGGGCCAGTTCAATTTTCCCAGATGAGGTACTGGAAAGAGTTTTGAGTTTGGTGAAGTCTCACAAAGATAGAAGCTCTGTATCTTTGGTTTGTAAAGATTGGTTCAACGCCGAGCGATGGTCTCGAACCCACGTTTTCATCGGGAATTGTTACTCTGTTTCGCCGGAAATTGTAATCCGACGGTTCCCGAACATTCGGAGTGTGACGCTGAAAGGGAAACCTAGGTTTTCAGATTTCAATTTGGTGCCTCCAAATTGGGGAGCTGATATTCATTCATGGCTTGTTGCCTTCGCTTCAAAATACCCAATTCTCGAAGAATTGAGGCTCAAGAGAATGGCTGTAACTGACGAAAGCTTAGAGTTTTTGGGTCGTTCCTTCCCAAATTTCAAAGCCCTTTCAATGATGAGCTGCGATGGCTTCAGCACCGATGGCCTTGCCGCCATTGCCACTCACTGCAAGAACTTGACGGAGCTTGACATACTTGAGAATGACATTATTGACAAAAGTGGAAATTGGCTGAGTTGCTTCCCAGATACCTTGAAATCTCTGGAAGTACTCAACTTTTCAAGTTTGAACAGCGATGTCAGTTTTGAGGCCCTTGAGAAGCTGGTAAGAAGGTGCAAATCACTGAAGGTTTTGAAGGTCAATAGGAATATAAACTTGGAACAACTGCAAAGGCTGCTTGTTCACACTCCTCAGTTAACGGAGCTAGGCACGGGCTCTTTTTCGCAAGAGATTACTCTTCGGCAGTACTGCGACCTCGAAGATGCTTTTAAAAGTTGCAAGAATCTGCGTACTCTCTCTGGTTTGTTGGAGTCGACAGTGTTGTATCTCCAAGTTCTTTTTCCTGCCTGTGCAAATTTAACTTTCTTGAATCTGAGCTACGCTATTCTTCATGGTGGTGAACTTGCTGGACTTCTTTCCCACTGCCCTGTTCTTCACCGCCTTTGGGTTTTAGACACAGTTGAAGACAAGGGCCTGAAGGCTGTTGGACTGAGTTGCCCCTTGCTGGAGGAACTCCGTGTATTTCCAGCAGATCCATTTGCTGACAATTTAGTCCATGGTGTAACCGAGTCTGGGTTTCTTGCTGTCTCTTATGGCTGCCGTAAACTCCATTACGTTCTCTACTTTTGCCGTCAGATGACGAATGAGGCTGTGGCCACCATTGTGCAGAACTGCCCAGATTTCACCCATTTTCGTCTCTGCATTATGAACCCCCACCAACCAGACTATCTTACAAAGCAACCAATGGATGAGGCCTTTGGTGCGGTGGTGAAAACTTGCTCTAGGCTGCGCAGACTTGCCATTTCGGGCTTGCTAACGGACTTAACATTTGAATACATTGGGAAATATGCAAAAAACTTGGAAACTCTTTCAGTGGCTTTTGCTGGAAGCAGTGACTGGGGAATGCAGTGTGTTATGAGTGGTTGTCCAAAGCTGAGAAAACTGGAGATCAGAGACAGCCCATTTGGAAATGCAGCTTTGCTTTCTGGTTTGGAGAGGTACGAATCCATGAGATCACTATGGATGTCGGCCTGCAAGGTGACGATGAACGGGTGTCGAGTTTTGGCAAAGCAGGTGCCGAGGTTGAACGTTGAGGTCATTAAGGATGGTGGAAGTGATGAATGCGAGGCTGAAAGTGTTTACGTATACCGCTCTGTTGCAGGTCCACGAAGAGACGCCCCGTCGTTCGTTCTCACCCTCTAA

Protein sequence

MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL
Homology
BLAST of HG10016122 vs. NCBI nr
Match: XP_038883074.1 (protein TRANSPORT INHIBITOR RESPONSE 1-like [Benincasa hispida])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 565/584 (96.75%), Postives = 573/584 (98.12%), Query Frame = 0

Query: 1   MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60
           MS KR+KGSGDSD+ NR  SIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH
Sbjct: 1   MSSKRQKGSGDSDESNRVGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60

Query: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPIL 120
           VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYP L
Sbjct: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPNL 120

Query: 121 EELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180
           EELRLKRM VTDESLEFL RSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND
Sbjct: 121 EELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180

Query: 181 IIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240
           I DKSG+WLSCFPDTLKSLEVLNFSSLNSD+SFEALEKLVRRCKSLKVLKVNRNINLEQL
Sbjct: 181 INDKSGHWLSCFPDTLKSLEVLNFSSLNSDISFEALEKLVRRCKSLKVLKVNRNINLEQL 240

Query: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300
           QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA
Sbjct: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300

Query: 301 CANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360
           CANLTFLNLSY+ILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRVFP
Sbjct: 301 CANLTFLNLSYSILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360

Query: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420
           ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM
Sbjct: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420

Query: 421 NPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480
           NPHQPDYLT+Q MDEAFGAVVKTCS+LRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG
Sbjct: 421 NPHQPDYLTEQSMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480

Query: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540
           SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA
Sbjct: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540

Query: 541 KQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           KQVPRLN+EVIKDGGSDECEAESVYVYRSVAGPRRDAP FVLTL
Sbjct: 541 KQVPRLNIEVIKDGGSDECEAESVYVYRSVAGPRRDAPPFVLTL 584

BLAST of HG10016122 vs. NCBI nr
Match: XP_008439908.1 (PREDICTED: protein AUXIN SIGNALING F-BOX 2-like [Cucumis melo] >TYK13102.1 protein AUXIN SIGNALING F-BOX 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 558/584 (95.55%), Postives = 570/584 (97.60%), Query Frame = 0

Query: 1   MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60
           MS KR+KGSGDSD+ NRA SIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH
Sbjct: 1   MSSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60

Query: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPIL 120
           VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGAD+HSWLVAFASKYPIL
Sbjct: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVAFASKYPIL 120

Query: 121 EELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180
           EELRLKRM VTDESLEFL RSFPNFKALSMMSCDGFSTDGLAAIAT+CKNLTELDILEND
Sbjct: 121 EELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILEND 180

Query: 181 IIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240
           I DKSGNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL
Sbjct: 181 INDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240

Query: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300
           QRLLVHTPQLTELG GSFSQEITLRQYCDLE+AFKSCKNL TLSGLLESTVLYLQVLFPA
Sbjct: 241 QRLLVHTPQLTELGMGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPA 300

Query: 301 CANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360
           CAN+TFLNLSYAILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRVFP
Sbjct: 301 CANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360

Query: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420
           A PFADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIM
Sbjct: 361 AHPFADNLVHGVTESGFLAVSYGCRKLRYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIM 420

Query: 421 NPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480
           NPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG
Sbjct: 421 NPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480

Query: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540
           S+DWGMQCVMSGCPKL+KLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA
Sbjct: 481 STDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540

Query: 541 KQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           KQVPRLNVEVIKD G+DECEAESVYVYRSVAGPRRDAP FV+TL
Sbjct: 541 KQVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVVTL 584

BLAST of HG10016122 vs. NCBI nr
Match: XP_004134961.1 (protein AUXIN SIGNALING F-BOX 2 [Cucumis sativus] >XP_031743133.1 protein AUXIN SIGNALING F-BOX 2 [Cucumis sativus] >KGN49186.1 hypothetical protein Csa_004088 [Cucumis sativus])

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 555/584 (95.03%), Postives = 567/584 (97.09%), Query Frame = 0

Query: 1   MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60
           MS KR+KGSGDSD+ NRA SIFPDEVLERVLSLVKSH+DRSSVSLVCKDWFNAERWSRTH
Sbjct: 1   MSSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHRDRSSVSLVCKDWFNAERWSRTH 60

Query: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPIL 120
           VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVP +WGADIHSWLVAFASKYPIL
Sbjct: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPSDWGADIHSWLVAFASKYPIL 120

Query: 121 EELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180
           EELRLKRM V DESLEFL RSFPNFKALSMMSCDGFSTDGLAAIAT+CKNLTELDILEND
Sbjct: 121 EELRLKRMTVMDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILEND 180

Query: 181 IIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240
           I DKSGNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRR KSLKVLKVNRNINLEQL
Sbjct: 181 INDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRSKSLKVLKVNRNINLEQL 240

Query: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300
           QRLLVHTPQLTELGTGSFSQEITLRQYCDLE+AFKSCKNL TLSGLLESTVLYLQVLFPA
Sbjct: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPA 300

Query: 301 CANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360
           CAN+TFLNLSYAILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRVFP
Sbjct: 301 CANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360

Query: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420
           A PFADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIM
Sbjct: 361 AHPFADNLVHGVTESGFLAVSYGCRKLCYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIM 420

Query: 421 NPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480
           NPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG
Sbjct: 421 NPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480

Query: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540
            +DWGMQCVMSGCPKL+KLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA
Sbjct: 481 RTDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540

Query: 541 KQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           KQVPRLNVEVIKD G+DECEAESVYVYRSVAGPRRDAP FVLTL
Sbjct: 541 KQVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVLTL 584

BLAST of HG10016122 vs. NCBI nr
Match: KAG7033903.1 (Transport inhibitor response 1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1125.5 bits (2910), Expect = 0.0e+00
Identity = 553/586 (94.37%), Postives = 565/586 (96.42%), Query Frame = 0

Query: 1   MSLKRKK--GSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSR 60
           MS KRKK  GSGDSD+  R  SIFPDEVLERVL LVKS KDRSSVSLVCKDW+NAERWSR
Sbjct: 1   MSSKRKKKGGSGDSDESTRGGSIFPDEVLERVLGLVKSRKDRSSVSLVCKDWYNAERWSR 60

Query: 61  THVFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYP 120
           THVFIGNCYSVSPEIVIRRFPNIRSV+LKGKPRFSDFNLVPPNWGADIHSWLV FASKYP
Sbjct: 61  THVFIGNCYSVSPEIVIRRFPNIRSVSLKGKPRFSDFNLVPPNWGADIHSWLVVFASKYP 120

Query: 121 ILEELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180
            LEELRLKRM VTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE
Sbjct: 121 NLEELRLKRMTVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180

Query: 181 NDIIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLE 240
           NDI DKSGNWLSCFPDTLKSL+VLNF+SLNSDVSFE+LEKLVRRCKSLKVLKVNRNINLE
Sbjct: 181 NDINDKSGNWLSCFPDTLKSLQVLNFASLNSDVSFESLEKLVRRCKSLKVLKVNRNINLE 240

Query: 241 QLQRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLF 300
           QLQRLLV+ PQLTELGTGSFSQE+TLRQY DLEDAFKSC NL TLSGLLESTVLYLQVLF
Sbjct: 241 QLQRLLVNAPQLTELGTGSFSQELTLRQYYDLEDAFKSCNNLHTLSGLLESTVLYLQVLF 300

Query: 301 PACANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRV 360
           PA ANLTFLNLSYAILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRV
Sbjct: 301 PASANLTFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRV 360

Query: 361 FPADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLC 420
           FPADP+ADNLVHGVTESGFLAVSYGC KLHYVLYFC QMTNEAVATIVQNCPDFTHFRLC
Sbjct: 361 FPADPYADNLVHGVTESGFLAVSYGCPKLHYVLYFCHQMTNEAVATIVQNCPDFTHFRLC 420

Query: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAF 480
           IMNPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTD+TFEYIGKYAKNLETLSVAF
Sbjct: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDITFEYIGKYAKNLETLSVAF 480

Query: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRV 540
           AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCR+
Sbjct: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRI 540

Query: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL
Sbjct: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 586

BLAST of HG10016122 vs. NCBI nr
Match: XP_022977973.1 (transport inhibitor response 1-like protein Os04g0395600 isoform X1 [Cucurbita maxima] >XP_022977974.1 transport inhibitor response 1-like protein Os04g0395600 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 553/586 (94.37%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MSLKRKK--GSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSR 60
           MS KRKK  GSGDSD+  RA SIFPDEVLERVLSLVKS KDRSSVSLVCKDWFNAERWSR
Sbjct: 1   MSSKRKKKGGSGDSDESTRAGSIFPDEVLERVLSLVKSRKDRSSVSLVCKDWFNAERWSR 60

Query: 61  THVFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYP 120
           THVFIGNCYSVSPEIVIRRFPNIRSV+LKGKPRFSDFNLVPPNWGADIHSWLV FASKYP
Sbjct: 61  THVFIGNCYSVSPEIVIRRFPNIRSVSLKGKPRFSDFNLVPPNWGADIHSWLVGFASKYP 120

Query: 121 ILEELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180
            LEELRLKRM VTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE
Sbjct: 121 NLEELRLKRMTVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180

Query: 181 NDIIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLE 240
           NDI DKSGNWLSCFPDTLKSL+VLNF+SLNSDVSFE+LEKLV+RCKSLKVLKVNRNINLE
Sbjct: 181 NDINDKSGNWLSCFPDTLKSLQVLNFASLNSDVSFESLEKLVKRCKSLKVLKVNRNINLE 240

Query: 241 QLQRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLF 300
           QLQRLLV+ PQLTELGTGSFSQ++TLRQY DLEDAFKSC NL TLSGLLESTVLYLQVLF
Sbjct: 241 QLQRLLVNAPQLTELGTGSFSQDLTLRQYYDLEDAFKSCNNLHTLSGLLESTVLYLQVLF 300

Query: 301 PACANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRV 360
           PA ANLTFLNLSYAILH GELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRV
Sbjct: 301 PASANLTFLNLSYAILHSGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRV 360

Query: 361 FPADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLC 420
           FPADP+ADNLVHGVTESGFLAVSYGC KLHYVLYFC QMTNEAVATIVQNCPDFTHFRLC
Sbjct: 361 FPADPYADNLVHGVTESGFLAVSYGCPKLHYVLYFCHQMTNEAVATIVQNCPDFTHFRLC 420

Query: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAF 480
           IMNPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTD+TFEYIGKYAKNLETLSVAF
Sbjct: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDITFEYIGKYAKNLETLSVAF 480

Query: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRV 540
           AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCR+
Sbjct: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRI 540

Query: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL
Sbjct: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 586

BLAST of HG10016122 vs. ExPASy Swiss-Prot
Match: Q570C0 (Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 PE=1 SV=2)

HSP 1 Score: 654.1 bits (1686), Expect = 1.5e-186
Identity = 320/570 (56.14%), Postives = 399/570 (70.00%), Query Frame = 0

Query: 17  RASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIR 76
           R +  FP+EVLE V S ++  KDR+SVSLVCK W+  ERW R  VFIGNCY+VSP  VIR
Sbjct: 4   RIALSFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIR 63

Query: 77  RFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLE 136
           RFP +RSV LKGKP F+DFNLVP  WG  ++ W+ A +S Y  LEE+RLKRM VTD+ LE
Sbjct: 64  RFPKVRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLE 123

Query: 137 FLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTL 196
            + +SF NFK L + SC+GFSTDGLAAIA  C+NL ELD+ E+D+ D SG+WLS FPDT 
Sbjct: 124 LIAKSFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTY 183

Query: 197 KSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTG 256
            SL  LN S L S+VSF ALE+LV RC +LK LK+NR + LE+L  LL   PQL ELGTG
Sbjct: 184 TSLVSLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTG 243

Query: 257 SFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAILHG 316
            ++ E+    Y  L  A   CK LR LSG  ++   YL  ++  C+ LT LNLSYA +  
Sbjct: 244 GYTAEVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQS 303

Query: 317 GELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESG 376
            +L  LL  CP L RLWVLD +ED GL+ +  +C  L ELRVFP++PF       +TE G
Sbjct: 304 YDLVKLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQG 363

Query: 377 FLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEA 436
            ++VS GC KL  VLYFCRQMTN A+ TI +N P+ T FRLCI+ P  PDYLT +P+D  
Sbjct: 364 LVSVSMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIG 423

Query: 437 FGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKL 496
           FGA+V+ C  LRRL++SGLLTD  FEYIG YAK +E LSVAFAG SD GM  V+SGC  L
Sbjct: 424 FGAIVEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSL 483

Query: 497 RKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGGS 556
           RKLEIRD PFG+ ALL+   + E+MRSLWMS+C V+   C++L +++P+LNVEVI + G+
Sbjct: 484 RKLEIRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGA 543

Query: 557 -----DECEAESVYVYRSVAGPRRDAPSFV 582
                + C  E V++YR+VAGPR D P FV
Sbjct: 544 PDSRPESCPVERVFIYRTVAGPRFDMPGFV 573

BLAST of HG10016122 vs. ExPASy Swiss-Prot
Match: Q9LW29 (Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 1.8e-182
Identity = 306/573 (53.40%), Postives = 404/573 (70.51%), Query Frame = 0

Query: 22  FPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 81
           FPDEV+E V   V SHKDR+++SLVCK W+  ER+SR  VFIGNCY+++PE ++RRFP +
Sbjct: 4   FPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCL 63

Query: 82  RSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRS 141
           +S+TLKGKP F+DFNLVP  WG  +  W+ A A     LEELRLKRM VTDESLE L RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRS 123

Query: 142 FPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 201
           F NFK+L ++SC+GF+TDGLA+IA +C++L +LD+ EN+I D  G WLSCFPDT  +L  
Sbjct: 124 FVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVT 183

Query: 202 LNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQE 261
           LNF+ L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQ+ +LG GS+  +
Sbjct: 184 LNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYEND 243

Query: 262 ITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAI-LHGGELA 321
                Y  L    K C +LR+LSG LE+    L    P C NLT LNLSYA  +HG  L 
Sbjct: 244 PDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLI 303

Query: 322 GLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAV 381
            L+ HC  L RLW+LD++ DKGL+ V  +C  L+ELRVFP+D         VTE G +A+
Sbjct: 304 KLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNT-AVTEEGLVAI 363

Query: 382 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAV 441
           S GC KLH +LYFC+QMTN A+ T+ +NCP+F  FRLCI+ P++PD++T QP+DE FGA+
Sbjct: 364 SAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAI 423

Query: 442 VKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLE 501
           VK C  LRRL++SGLLTD  F YIG YA  LE LS+AFAG +D GM  V++GC K++KLE
Sbjct: 424 VKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLE 483

Query: 502 IRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGGSDECE 561
           IRDSPFG+ ALL+ + +YE+MRSLWMS+C+VT++GC+ LA++ P LNVE+I +  ++  E
Sbjct: 484 IRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRME 543

Query: 562 ---------AESVYVYRSVAGPRRDAPSFVLTL 585
                     + +Y+YR+V G R DAP FV  L
Sbjct: 544 ENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of HG10016122 vs. ExPASy Swiss-Prot
Match: Q7XVM8 (Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0395600 PE=2 SV=1)

HSP 1 Score: 634.4 bits (1635), Expect = 1.3e-180
Identity = 310/573 (54.10%), Postives = 408/573 (71.20%), Query Frame = 0

Query: 22  FPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 81
           FP+EV+E + S + + +DR++VSLVCK W+  ER SR  VF+GNCY+V    V  RFPN+
Sbjct: 4   FPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVGNCYAVRAGRVAARFPNV 63

Query: 82  RSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRS 141
           R++T+KGKP F+DFNLVPP+WG     W+ A A     LEELR+KRM V+DESLE L RS
Sbjct: 64  RALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEELRMKRMVVSDESLELLARS 123

Query: 142 FPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 201
           FP F+AL ++SC+GFSTDGLAA+A+HCK L ELD+ EN++ D+   WLSCFPD+  SL  
Sbjct: 124 FPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVEDRGPRWLSCFPDSCTSLVS 183

Query: 202 LNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQE 261
           LNF+ +  +V+  +LE+LV R  +L+ L++NR+++++ L ++L+ TP L +LGTG+ + +
Sbjct: 184 LNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLAKILLRTPNLEDLGTGNLTDD 243

Query: 262 ITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYA-ILHGGELA 321
                Y  L  A + CK LR+LSG  +++ + L  ++P CA LT LNLSYA  L   +L 
Sbjct: 244 FQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPLCAQLTGLNLSYAPTLDASDLT 303

Query: 322 GLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAV 381
            ++S C  L RLWVLD + DKGL+ V  SC  L+ELRVFP+D +       VTE G +AV
Sbjct: 304 KMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRVFPSDFYVAG-YSAVTEEGLVAV 363

Query: 382 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAV 441
           S GC KL+ +LYFC QMTN A+ T+ +NCP+FT FRLCI+ P +PD +T QP+DE FGA+
Sbjct: 364 SLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLCILEPGKPDVVTSQPLDEGFGAI 423

Query: 442 VKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLE 501
           V+ C  L+RL+ISGLLTD  F YIGKYAK LE LS+AFAG SD GM  VM+GC  LRKLE
Sbjct: 424 VRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIAFAGDSDKGMMHVMNGCKNLRKLE 483

Query: 502 IRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKD-GGSDEC 561
           IRDSPFG+AALL    RYE+MRSLWMS+C VT+ GC+VLA ++P LNVEVI +  GS+E 
Sbjct: 484 IRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGCQVLASKMPMLNVEVINERDGSNEM 543

Query: 562 E--------AESVYVYRSVAGPRRDAPSFVLTL 585
           E         E +YVYR+ AG R DAP+FV  L
Sbjct: 544 EENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL 575

BLAST of HG10016122 vs. ExPASy Swiss-Prot
Match: Q9LPW7 (Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.7e-180
Identity = 311/580 (53.62%), Postives = 404/580 (69.66%), Query Frame = 0

Query: 22  FPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 81
           FPDEV+E V   V SHKDR+S+SLVCK W   ER+SR  VFIGNCY+++PE +IRRFP +
Sbjct: 4   FPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCL 63

Query: 82  RSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRS 141
           +S+TLKGKP F+DFNLVP  WG  +H W+ A A     LEELRLKRM VTDESL+ L RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRS 123

Query: 142 FPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 201
           F NFK+L ++SC+GF+TDGLA+IA +C++L ELD+ EN+I D  G WL+CFPD+  +L  
Sbjct: 124 FANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTTLMS 183

Query: 202 LNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQE 261
           LNF+ L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQL +LG GS+  E
Sbjct: 184 LNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSYENE 243

Query: 262 ITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAI-LHGGELA 321
                +  L  A K   +LR+LSG LE   L L   +P C NL  LNLSYA  + G  L 
Sbjct: 244 PDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGNHLI 303

Query: 322 GLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHG-------VT 381
            L+  C  L RLW+LD++ DKGL  V  +C  L+ELRVFP+D      VHG       VT
Sbjct: 304 KLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSD------VHGEEDNNASVT 363

Query: 382 ESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPM 441
           E G +A+S GC KLH +LYFC+QMTN A+  + +NCP+F  FRLCI+ PH+PD++T Q +
Sbjct: 364 EVGLVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSL 423

Query: 442 DEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGC 501
           DE FGA+V+ C  LRRL++SGLLTD  F YIG YA+ LE LS+AFAG +D GM  V++GC
Sbjct: 424 DEGFGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGC 483

Query: 502 PKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKD 561
            K+RKLEIRDSPFGNAALL+ + RYE+MRSLWMS+C+VT+ GC+ LA+  PRLNVE+I +
Sbjct: 484 KKMRKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINE 543

Query: 562 GGSDECE---------AESVYVYRSVAGPRRDAPSFVLTL 585
             ++  E          + +Y+YR+V G R+DAP +V  L
Sbjct: 544 NENNGMEQNEEDEREKVDKLYLYRTVVGTRKDAPPYVRIL 577

BLAST of HG10016122 vs. ExPASy Swiss-Prot
Match: Q0DKP3 (Transport inhibitor response 1-like protein Os05g0150500 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0150500 PE=2 SV=2)

HSP 1 Score: 585.1 bits (1507), Expect = 8.7e-166
Identity = 288/567 (50.79%), Postives = 376/567 (66.31%), Query Frame = 0

Query: 23  PDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNIR 82
           PDEV E   S + +  DR + +  C  W  AER SR  + + NCY+ +P   + RFP++R
Sbjct: 21  PDEVWEHAFSFLPAAADRGAAAGACSSWLRAERRSRRRLAVANCYAAAPRDAVERFPSVR 80

Query: 83  SVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRSF 142
           +  +KGKP F+DF LVPP WGA    W+ A A  +P+LEEL  KRM VTDE LE +  SF
Sbjct: 81  AAEVKGKPHFADFGLVPPAWGAAAAPWIAAAADGWPLLEELSFKRMVVTDECLEMIAASF 140

Query: 143 PNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEVL 202
            NF+ L ++SCDGFST GLAAIA  C++L ELD+ EN+I D S +WLS FP++  SL  L
Sbjct: 141 RNFQVLRLVSCDGFSTAGLAAIAAGCRHLRELDLQENEIEDCSIHWLSLFPESFTSLVTL 200

Query: 203 NFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQEI 262
           NFS L  +V+   LE+LV RC +LK LK+N  I L++L  LL   PQL ELGTG FS + 
Sbjct: 201 NFSCLEGEVNITVLERLVTRCHNLKTLKLNNAIPLDKLASLLHKAPQLVELGTGKFSADY 260

Query: 263 TLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAILHGGELAGL 322
               +  LE AF  CK+LR LSG  ++   YL   +  C  LT LNLSYA + G EL   
Sbjct: 261 HSDLFAKLEAAFGGCKSLRRLSGAWDAVPDYLPAFYCVCEGLTSLNLSYATVRGPELIKF 320

Query: 323 LSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAVSY 382
           +S C  L +LWV+D +ED GL  V  SC  L+ELRVFP+DPF       +TE G + VS 
Sbjct: 321 ISRCRNLQQLWVMDLIEDHGLAVVASSCNKLQELRVFPSDPFGAGF---LTERGLVDVSA 380

Query: 383 GCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAVVK 442
            C  L  VLYFCR+MTNEA+ TI +N P+FT FRLCI+ PH PDY+T++P+D  F A+V+
Sbjct: 381 SCPMLESVLYFCRRMTNEALITIAKNRPNFTCFRLCILEPHTPDYITREPLDAGFSAIVE 440

Query: 443 TCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLEIR 502
           +C  LRRL+ISGLLTDL F+ IG +A  LE LS+AFAG+SD G+  ++SGC  L+KLEIR
Sbjct: 441 SCRGLRRLSISGLLTDLVFKSIGAHADRLEMLSIAFAGNSDLGLHYILSGCKSLKKLEIR 500

Query: 503 DSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGG------- 562
           D PFG+  LL+   + E+MRSLWMS+C +T+  CR LA+++PRL+VE++ D G       
Sbjct: 501 DCPFGDKPLLANAAKLETMRSLWMSSCLLTLGACRQLARKMPRLSVEIMNDPGRSCPLDS 560

Query: 563 -SDECEAESVYVYRSVAGPRRDAPSFV 582
             DE   E +YVYR++AGPR D P+ V
Sbjct: 561 LPDETPVEKLYVYRTIAGPRSDTPACV 584

BLAST of HG10016122 vs. ExPASy TrEMBL
Match: A0A5D3CP23 (Protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007110 PE=4 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 558/584 (95.55%), Postives = 570/584 (97.60%), Query Frame = 0

Query: 1   MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60
           MS KR+KGSGDSD+ NRA SIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH
Sbjct: 1   MSSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60

Query: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPIL 120
           VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGAD+HSWLVAFASKYPIL
Sbjct: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVAFASKYPIL 120

Query: 121 EELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180
           EELRLKRM VTDESLEFL RSFPNFKALSMMSCDGFSTDGLAAIAT+CKNLTELDILEND
Sbjct: 121 EELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILEND 180

Query: 181 IIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240
           I DKSGNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL
Sbjct: 181 INDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240

Query: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300
           QRLLVHTPQLTELG GSFSQEITLRQYCDLE+AFKSCKNL TLSGLLESTVLYLQVLFPA
Sbjct: 241 QRLLVHTPQLTELGMGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPA 300

Query: 301 CANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360
           CAN+TFLNLSYAILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRVFP
Sbjct: 301 CANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360

Query: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420
           A PFADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIM
Sbjct: 361 AHPFADNLVHGVTESGFLAVSYGCRKLRYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIM 420

Query: 421 NPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480
           NPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG
Sbjct: 421 NPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480

Query: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540
           S+DWGMQCVMSGCPKL+KLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA
Sbjct: 481 STDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540

Query: 541 KQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           KQVPRLNVEVIKD G+DECEAESVYVYRSVAGPRRDAP FV+TL
Sbjct: 541 KQVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVVTL 584

BLAST of HG10016122 vs. ExPASy TrEMBL
Match: A0A1S3B0L8 (protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo OX=3656 GN=LOC103484554 PE=4 SV=1)

HSP 1 Score: 1142.5 bits (2954), Expect = 0.0e+00
Identity = 558/584 (95.55%), Postives = 570/584 (97.60%), Query Frame = 0

Query: 1   MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60
           MS KR+KGSGDSD+ NRA SIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH
Sbjct: 1   MSSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60

Query: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPIL 120
           VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGAD+HSWLVAFASKYPIL
Sbjct: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADVHSWLVAFASKYPIL 120

Query: 121 EELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180
           EELRLKRM VTDESLEFL RSFPNFKALSMMSCDGFSTDGLAAIAT+CKNLTELDILEND
Sbjct: 121 EELRLKRMTVTDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILEND 180

Query: 181 IIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240
           I DKSGNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL
Sbjct: 181 INDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240

Query: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300
           QRLLVHTPQLTELG GSFSQEITLRQYCDLE+AFKSCKNL TLSGLLESTVLYLQVLFPA
Sbjct: 241 QRLLVHTPQLTELGMGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPA 300

Query: 301 CANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360
           CAN+TFLNLSYAILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRVFP
Sbjct: 301 CANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360

Query: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420
           A PFADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIM
Sbjct: 361 AHPFADNLVHGVTESGFLAVSYGCRKLRYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIM 420

Query: 421 NPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480
           NPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG
Sbjct: 421 NPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480

Query: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540
           S+DWGMQCVMSGCPKL+KLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA
Sbjct: 481 STDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540

Query: 541 KQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           KQVPRLNVEVIKD G+DECEAESVYVYRSVAGPRRDAP FV+TL
Sbjct: 541 KQVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVVTL 584

BLAST of HG10016122 vs. ExPASy TrEMBL
Match: A0A0A0KN74 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G516940 PE=4 SV=1)

HSP 1 Score: 1130.9 bits (2924), Expect = 0.0e+00
Identity = 555/584 (95.03%), Postives = 567/584 (97.09%), Query Frame = 0

Query: 1   MSLKRKKGSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTH 60
           MS KR+KGSGDSD+ NRA SIFPDEVLERVLSLVKSH+DRSSVSLVCKDWFNAERWSRTH
Sbjct: 1   MSSKRRKGSGDSDESNRAGSIFPDEVLERVLSLVKSHRDRSSVSLVCKDWFNAERWSRTH 60

Query: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPIL 120
           VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVP +WGADIHSWLVAFASKYPIL
Sbjct: 61  VFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPSDWGADIHSWLVAFASKYPIL 120

Query: 121 EELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILEND 180
           EELRLKRM V DESLEFL RSFPNFKALSMMSCDGFSTDGLAAIAT+CKNLTELDILEND
Sbjct: 121 EELRLKRMTVMDESLEFLSRSFPNFKALSMMSCDGFSTDGLAAIATNCKNLTELDILEND 180

Query: 181 IIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQL 240
           I DKSGNWLSCFPDTLKSLEVLNF+SLNSDVSFEALEKLVRR KSLKVLKVNRNINLEQL
Sbjct: 181 INDKSGNWLSCFPDTLKSLEVLNFASLNSDVSFEALEKLVRRSKSLKVLKVNRNINLEQL 240

Query: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPA 300
           QRLLVHTPQLTELGTGSFSQEITLRQYCDLE+AFKSCKNL TLSGLLESTVLYLQVLFPA
Sbjct: 241 QRLLVHTPQLTELGTGSFSQEITLRQYCDLEEAFKSCKNLHTLSGLLESTVLYLQVLFPA 300

Query: 301 CANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360
           CAN+TFLNLSYAILHGGELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRVFP
Sbjct: 301 CANITFLNLSYAILHGGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRVFP 360

Query: 361 ADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIM 420
           A PFADNLVHGVTESGFLAVSYGCRKL YVLYFC QMTNEAVATIVQNCPDFTHFRLCIM
Sbjct: 361 AHPFADNLVHGVTESGFLAVSYGCRKLCYVLYFCHQMTNEAVATIVQNCPDFTHFRLCIM 420

Query: 421 NPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480
           NPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG
Sbjct: 421 NPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAG 480

Query: 481 SSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540
            +DWGMQCVMSGCPKL+KLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA
Sbjct: 481 RTDWGMQCVMSGCPKLKKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLA 540

Query: 541 KQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           KQVPRLNVEVIKD G+DECEAESVYVYRSVAGPRRDAP FVLTL
Sbjct: 541 KQVPRLNVEVIKDDGNDECEAESVYVYRSVAGPRRDAPPFVLTL 584

BLAST of HG10016122 vs. ExPASy TrEMBL
Match: A0A6J1ISU2 (transport inhibitor response 1-like protein Os04g0395600 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478106 PE=4 SV=1)

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 553/586 (94.37%), Postives = 566/586 (96.59%), Query Frame = 0

Query: 1   MSLKRKK--GSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSR 60
           MS KRKK  GSGDSD+  RA SIFPDEVLERVLSLVKS KDRSSVSLVCKDWFNAERWSR
Sbjct: 1   MSSKRKKKGGSGDSDESTRAGSIFPDEVLERVLSLVKSRKDRSSVSLVCKDWFNAERWSR 60

Query: 61  THVFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYP 120
           THVFIGNCYSVSPEIVIRRFPNIRSV+LKGKPRFSDFNLVPPNWGADIHSWLV FASKYP
Sbjct: 61  THVFIGNCYSVSPEIVIRRFPNIRSVSLKGKPRFSDFNLVPPNWGADIHSWLVGFASKYP 120

Query: 121 ILEELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180
            LEELRLKRM VTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE
Sbjct: 121 NLEELRLKRMTVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180

Query: 181 NDIIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLE 240
           NDI DKSGNWLSCFPDTLKSL+VLNF+SLNSDVSFE+LEKLV+RCKSLKVLKVNRNINLE
Sbjct: 181 NDINDKSGNWLSCFPDTLKSLQVLNFASLNSDVSFESLEKLVKRCKSLKVLKVNRNINLE 240

Query: 241 QLQRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLF 300
           QLQRLLV+ PQLTELGTGSFSQ++TLRQY DLEDAFKSC NL TLSGLLESTVLYLQVLF
Sbjct: 241 QLQRLLVNAPQLTELGTGSFSQDLTLRQYYDLEDAFKSCNNLHTLSGLLESTVLYLQVLF 300

Query: 301 PACANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRV 360
           PA ANLTFLNLSYAILH GELAGLLSHCPVL RLWVLDTVEDKGLKAVGLSCPLLEELRV
Sbjct: 301 PASANLTFLNLSYAILHSGELAGLLSHCPVLRRLWVLDTVEDKGLKAVGLSCPLLEELRV 360

Query: 361 FPADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLC 420
           FPADP+ADNLVHGVTESGFLAVSYGC KLHYVLYFC QMTNEAVATIVQNCPDFTHFRLC
Sbjct: 361 FPADPYADNLVHGVTESGFLAVSYGCPKLHYVLYFCHQMTNEAVATIVQNCPDFTHFRLC 420

Query: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAF 480
           IMNPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTD+TFEYIGKYAKNLETLSVAF
Sbjct: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDITFEYIGKYAKNLETLSVAF 480

Query: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRV 540
           AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCR+
Sbjct: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRI 540

Query: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL
Sbjct: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 586

BLAST of HG10016122 vs. ExPASy TrEMBL
Match: A0A6J1GCV4 (transport inhibitor response 1-like protein Os04g0395600 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453032 PE=4 SV=1)

HSP 1 Score: 1125.2 bits (2909), Expect = 0.0e+00
Identity = 553/586 (94.37%), Postives = 564/586 (96.25%), Query Frame = 0

Query: 1   MSLKRKK--GSGDSDDLNRASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSR 60
           MS KRKK  GSGDSD+  R  SIFPDEVLERVL LVKS KDRSSVSLVCKDWFNAERWSR
Sbjct: 1   MSSKRKKKGGSGDSDESTRGGSIFPDEVLERVLGLVKSRKDRSSVSLVCKDWFNAERWSR 60

Query: 61  THVFIGNCYSVSPEIVIRRFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYP 120
           THVFIGNCYSVSPEIVIRRFPNIRSV+LKGKPRFSDFNLVPPNWGADIHSWLV FASKYP
Sbjct: 61  THVFIGNCYSVSPEIVIRRFPNIRSVSLKGKPRFSDFNLVPPNWGADIHSWLVVFASKYP 120

Query: 121 ILEELRLKRMAVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180
            LEELRLKRM VTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE
Sbjct: 121 NLEELRLKRMTVTDESLEFLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILE 180

Query: 181 NDIIDKSGNWLSCFPDTLKSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLE 240
           NDI DKSGNWLSCFPDTLKSL+VLNF+SLNSDVSFE+LEKLVRRCKSLKVLKVNRNINLE
Sbjct: 181 NDINDKSGNWLSCFPDTLKSLQVLNFASLNSDVSFESLEKLVRRCKSLKVLKVNRNINLE 240

Query: 241 QLQRLLVHTPQLTELGTGSFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLF 300
           QLQRLLV+ PQLTELGTGSFSQE+TLRQY DLEDAFKSC NL TLSGLLESTVLYLQVLF
Sbjct: 241 QLQRLLVNAPQLTELGTGSFSQELTLRQYYDLEDAFKSCNNLHTLSGLLESTVLYLQVLF 300

Query: 301 PACANLTFLNLSYAILHGGELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRV 360
           PA ANLTFLNLSYAILHGGELAGLLSHCP L RLWVLDTVEDKGLKAVGLSCPLLEELRV
Sbjct: 301 PASANLTFLNLSYAILHGGELAGLLSHCPALRRLWVLDTVEDKGLKAVGLSCPLLEELRV 360

Query: 361 FPADPFADNLVHGVTESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLC 420
           FPADP+ADNLVHGVTESGFLAVSYGC KLHYVLYFC QMTNEAVATIVQNCPDFTHFRLC
Sbjct: 361 FPADPYADNLVHGVTESGFLAVSYGCPKLHYVLYFCHQMTNEAVATIVQNCPDFTHFRLC 420

Query: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAF 480
           IMNPHQPDYLTKQPMDEAFGAVVKTCS+LRRLAISGLLTD+TFEYIGKYAKNLETLSVAF
Sbjct: 421 IMNPHQPDYLTKQPMDEAFGAVVKTCSKLRRLAISGLLTDITFEYIGKYAKNLETLSVAF 480

Query: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRV 540
           AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCR+
Sbjct: 481 AGSSDWGMQCVMSGCPKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRI 540

Query: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 585
           LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL
Sbjct: 541 LAKQVPRLNVEVIKDGGSDECEAESVYVYRSVAGPRRDAPSFVLTL 586

BLAST of HG10016122 vs. TAIR 10
Match: AT3G62980.1 (F-box/RNI-like superfamily protein )

HSP 1 Score: 654.1 bits (1686), Expect = 1.1e-187
Identity = 320/570 (56.14%), Postives = 399/570 (70.00%), Query Frame = 0

Query: 17  RASSIFPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIR 76
           R +  FP+EVLE V S ++  KDR+SVSLVCK W+  ERW R  VFIGNCY+VSP  VIR
Sbjct: 4   RIALSFPEEVLEHVFSFIQLDKDRNSVSLVCKSWYEIERWCRRKVFIGNCYAVSPATVIR 63

Query: 77  RFPNIRSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLE 136
           RFP +RSV LKGKP F+DFNLVP  WG  ++ W+ A +S Y  LEE+RLKRM VTD+ LE
Sbjct: 64  RFPKVRSVELKGKPHFADFNLVPDGWGGYVYPWIEAMSSSYTWLEEIRLKRMVVTDDCLE 123

Query: 137 FLGRSFPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTL 196
            + +SF NFK L + SC+GFSTDGLAAIA  C+NL ELD+ E+D+ D SG+WLS FPDT 
Sbjct: 124 LIAKSFKNFKVLVLSSCEGFSTDGLAAIAATCRNLKELDLRESDVDDVSGHWLSHFPDTY 183

Query: 197 KSLEVLNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTG 256
            SL  LN S L S+VSF ALE+LV RC +LK LK+NR + LE+L  LL   PQL ELGTG
Sbjct: 184 TSLVSLNISCLASEVSFSALERLVTRCPNLKSLKLNRAVPLEKLATLLQRAPQLEELGTG 243

Query: 257 SFSQEITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAILHG 316
            ++ E+    Y  L  A   CK LR LSG  ++   YL  ++  C+ LT LNLSYA +  
Sbjct: 244 GYTAEVRPDVYSGLSVALSGCKELRCLSGFWDAVPAYLPAVYSVCSRLTTLNLSYATVQS 303

Query: 317 GELAGLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESG 376
            +L  LL  CP L RLWVLD +ED GL+ +  +C  L ELRVFP++PF       +TE G
Sbjct: 304 YDLVKLLCQCPKLQRLWVLDYIEDAGLEVLASTCKDLRELRVFPSEPFVMEPNVALTEQG 363

Query: 377 FLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEA 436
            ++VS GC KL  VLYFCRQMTN A+ TI +N P+ T FRLCI+ P  PDYLT +P+D  
Sbjct: 364 LVSVSMGCPKLESVLYFCRQMTNAALITIARNRPNMTRFRLCIIEPKAPDYLTLEPLDIG 423

Query: 437 FGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKL 496
           FGA+V+ C  LRRL++SGLLTD  FEYIG YAK +E LSVAFAG SD GM  V+SGC  L
Sbjct: 424 FGAIVEHCKDLRRLSLSGLLTDKVFEYIGTYAKKMEMLSVAFAGDSDLGMHHVLSGCDSL 483

Query: 497 RKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGGS 556
           RKLEIRD PFG+ ALL+   + E+MRSLWMS+C V+   C++L +++P+LNVEVI + G+
Sbjct: 484 RKLEIRDCPFGDKALLANASKLETMRSLWMSSCSVSFGACKLLGQKMPKLNVEVIDERGA 543

Query: 557 -----DECEAESVYVYRSVAGPRRDAPSFV 582
                + C  E V++YR+VAGPR D P FV
Sbjct: 544 PDSRPESCPVERVFIYRTVAGPRFDMPGFV 573

BLAST of HG10016122 vs. TAIR 10
Match: AT3G26810.1 (auxin signaling F-box 2 )

HSP 1 Score: 640.6 bits (1651), Expect = 1.2e-183
Identity = 306/573 (53.40%), Postives = 404/573 (70.51%), Query Frame = 0

Query: 22  FPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 81
           FPDEV+E V   V SHKDR+++SLVCK W+  ER+SR  VFIGNCY+++PE ++RRFP +
Sbjct: 4   FPDEVIEHVFDFVTSHKDRNAISLVCKSWYKIERYSRQKVFIGNCYAINPERLLRRFPCL 63

Query: 82  RSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRS 141
           +S+TLKGKP F+DFNLVP  WG  +  W+ A A     LEELRLKRM VTDESLE L RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVLPWIEALARSRVGLEELRLKRMVVTDESLELLSRS 123

Query: 142 FPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 201
           F NFK+L ++SC+GF+TDGLA+IA +C++L +LD+ EN+I D  G WLSCFPDT  +L  
Sbjct: 124 FVNFKSLVLVSCEGFTTDGLASIAANCRHLRDLDLQENEIDDHRGQWLSCFPDTCTTLVT 183

Query: 202 LNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQE 261
           LNF+ L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQ+ +LG GS+  +
Sbjct: 184 LNFACLEGETNLVALERLVARSPNLKSLKLNRAVPLDALARLMACAPQIVDLGVGSYEND 243

Query: 262 ITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAI-LHGGELA 321
                Y  L    K C +LR+LSG LE+    L    P C NLT LNLSYA  +HG  L 
Sbjct: 244 PDSESYLKLMAVIKKCTSLRSLSGFLEAAPHCLSAFHPICHNLTSLNLSYAAEIHGSHLI 303

Query: 322 GLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAV 381
            L+ HC  L RLW+LD++ DKGL+ V  +C  L+ELRVFP+D         VTE G +A+
Sbjct: 304 KLIQHCKKLQRLWILDSIGDKGLEVVASTCKELQELRVFPSDLLGGGNT-AVTEEGLVAI 363

Query: 382 SYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAV 441
           S GC KLH +LYFC+QMTN A+ T+ +NCP+F  FRLCI+ P++PD++T QP+DE FGA+
Sbjct: 364 SAGCPKLHSILYFCQQMTNAALVTVAKNCPNFIRFRLCILEPNKPDHVTSQPLDEGFGAI 423

Query: 442 VKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLE 501
           VK C  LRRL++SGLLTD  F YIG YA  LE LS+AFAG +D GM  V++GC K++KLE
Sbjct: 424 VKACKSLRRLSLSGLLTDQVFLYIGMYANQLEMLSIAFAGDTDKGMLYVLNGCKKMKKLE 483

Query: 502 IRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGGSDECE 561
           IRDSPFG+ ALL+ + +YE+MRSLWMS+C+VT++GC+ LA++ P LNVE+I +  ++  E
Sbjct: 484 IRDSPFGDTALLADVSKYETMRSLWMSSCEVTLSGCKRLAEKAPWLNVEIINENDNNRME 543

Query: 562 ---------AESVYVYRSVAGPRRDAPSFVLTL 585
                     + +Y+YR+V G R DAP FV  L
Sbjct: 544 ENGHEGRQKVDKLYLYRTVVGTRMDAPPFVWIL 575

BLAST of HG10016122 vs. TAIR 10
Match: AT1G12820.1 (auxin signaling F-box 3 )

HSP 1 Score: 632.9 bits (1631), Expect = 2.6e-181
Identity = 311/580 (53.62%), Postives = 404/580 (69.66%), Query Frame = 0

Query: 22  FPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 81
           FPDEV+E V   V SHKDR+S+SLVCK W   ER+SR  VFIGNCY+++PE +IRRFP +
Sbjct: 4   FPDEVIEHVFDFVASHKDRNSISLVCKSWHKIERFSRKEVFIGNCYAINPERLIRRFPCL 63

Query: 82  RSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRS 141
           +S+TLKGKP F+DFNLVP  WG  +H W+ A A     LEELRLKRM VTDESL+ L RS
Sbjct: 64  KSLTLKGKPHFADFNLVPHEWGGFVHPWIEALARSRVGLEELRLKRMVVTDESLDLLSRS 123

Query: 142 FPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 201
           F NFK+L ++SC+GF+TDGLA+IA +C++L ELD+ EN+I D  G WL+CFPD+  +L  
Sbjct: 124 FANFKSLVLVSCEGFTTDGLASIAANCRHLRELDLQENEIDDHRGQWLNCFPDSCTTLMS 183

Query: 202 LNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQE 261
           LNF+ L  + +  ALE+LV R  +LK LK+NR + L+ L RL+   PQL +LG GS+  E
Sbjct: 184 LNFACLKGETNVAALERLVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSYENE 243

Query: 262 ITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAI-LHGGELA 321
                +  L  A K   +LR+LSG LE   L L   +P C NL  LNLSYA  + G  L 
Sbjct: 244 PDPESFAKLMTAIKKYTSLRSLSGFLEVAPLCLPAFYPICQNLISLNLSYAAEIQGNHLI 303

Query: 322 GLLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHG-------VT 381
            L+  C  L RLW+LD++ DKGL  V  +C  L+ELRVFP+D      VHG       VT
Sbjct: 304 KLIQLCKRLQRLWILDSIGDKGLAVVAATCKELQELRVFPSD------VHGEEDNNASVT 363

Query: 382 ESGFLAVSYGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPM 441
           E G +A+S GC KLH +LYFC+QMTN A+  + +NCP+F  FRLCI+ PH+PD++T Q +
Sbjct: 364 EVGLVAISAGCPKLHSILYFCKQMTNAALIAVAKNCPNFIRFRLCILEPHKPDHITFQSL 423

Query: 442 DEAFGAVVKTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGC 501
           DE FGA+V+ C  LRRL++SGLLTD  F YIG YA+ LE LS+AFAG +D GM  V++GC
Sbjct: 424 DEGFGAIVQACKGLRRLSVSGLLTDQVFLYIGMYAEQLEMLSIAFAGDTDKGMLYVLNGC 483

Query: 502 PKLRKLEIRDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKD 561
            K+RKLEIRDSPFGNAALL+ + RYE+MRSLWMS+C+VT+ GC+ LA+  PRLNVE+I +
Sbjct: 484 KKMRKLEIRDSPFGNAALLADVGRYETMRSLWMSSCEVTLGGCKRLAQNSPRLNVEIINE 543

Query: 562 GGSDECE---------AESVYVYRSVAGPRRDAPSFVLTL 585
             ++  E          + +Y+YR+V G R+DAP +V  L
Sbjct: 544 NENNGMEQNEEDEREKVDKLYLYRTVVGTRKDAPPYVRIL 577

BLAST of HG10016122 vs. TAIR 10
Match: AT4G03190.1 (GRR1-like protein 1 )

HSP 1 Score: 583.6 bits (1503), Expect = 1.8e-166
Identity = 289/567 (50.97%), Postives = 384/567 (67.72%), Query Frame = 0

Query: 22  FPDEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNI 81
           FP +VLE +LS + S++DR+SVSLVCK WF  ER +R  VF+GNCY+VSP  V RRFP +
Sbjct: 5   FPPKVLEHILSFIDSNEDRNSVSLVCKSWFETERKTRKRVFVGNCYAVSPAAVTRRFPEM 64

Query: 82  RSVTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRS 141
           RS+TLKGKP F+D+NLVP  WG     W+ A A+K   LEE+R+KRM VTDE LE +  S
Sbjct: 65  RSLTLKGKPHFADYNLVPDGWGGYAWPWIEAMAAKSSSLEEIRMKRMVVTDECLEKIAAS 124

Query: 142 FPNFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEV 201
           F +FK L + SC+GFSTDG+AAIA  C+NL  L++ E  + D  G+WLS FP++  SL  
Sbjct: 125 FKDFKVLVLTSCEGFSTDGIAAIAATCRNLRVLELRECIVEDLGGDWLSYFPESSTSLVS 184

Query: 202 LNFSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQE 261
           L+FS L+S+V    LE+LV R  +LK LK+N  + L+ L  LL   PQLTELGTGSF+ +
Sbjct: 185 LDFSCLDSEVKISDLERLVSRSPNLKSLKLNPAVTLDGLVSLLRCAPQLTELGTGSFAAQ 244

Query: 262 ITLRQYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAILHGGELAG 321
           +    +  L +AF +CK L++LSGL +    YL  L+  C  LT LNLSYA +   +L  
Sbjct: 245 LKPEAFSKLSEAFSNCKQLQSLSGLWDVLPEYLPALYSVCPGLTSLNLSYATVRMPDLVE 304

Query: 322 LLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAVS 381
           LL  C  L +LWV+D +EDKGL+AV   C  L ELRVFP++P  D     +TE G + VS
Sbjct: 305 LLRRCSKLQKLWVMDLIEDKGLEAVASYCKELRELRVFPSEPDLDATNIPLTEQGLVFVS 364

Query: 382 YGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAVV 441
            GCRKL  VLYFC Q TN A+ TI +  P+   FRLC++ P  PDY T +P+D+ F A+ 
Sbjct: 365 KGCRKLESVLYFCVQFTNAALFTIARKRPNLKCFRLCVIEPFAPDYKTNEPLDKGFKAIA 424

Query: 442 KTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLEI 501
           + C  LRRL++SGLL+D  F+YIGK+AK +  LS+AFAG SD  +  ++SGC  L+KLEI
Sbjct: 425 EGCRDLRRLSVSGLLSDKAFKYIGKHAKKVRMLSIAFAGDSDLMLHHLLSGCESLKKLEI 484

Query: 502 RDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVI----KDGGSD 561
           RD PFG+ ALL    + E+MRSLWMS+C V+   C++L++++PRLNVEVI     +   +
Sbjct: 485 RDCPFGDTALLEHAAKLETMRSLWMSSCFVSFGACKLLSQKMPRLNVEVIDEHPPESRPE 544

Query: 562 ECEAESVYVYRSVAGPRRDAPSFVLTL 585
               E +Y+YR+VAGPR D P FV T+
Sbjct: 545 SSPVERIYIYRTVAGPRMDTPEFVWTI 571

BLAST of HG10016122 vs. TAIR 10
Match: AT4G24390.2 (RNI-like superfamily protein )

HSP 1 Score: 577.8 bits (1488), Expect = 9.9e-165
Identity = 281/569 (49.38%), Postives = 383/569 (67.31%), Query Frame = 0

Query: 24  DEVLERVLSLVKSHKDRSSVSLVCKDWFNAERWSRTHVFIGNCYSVSPEIVIRRFPNIRS 83
           + VLE VL  + S  DR++VSLVC+ W+  E  +R  VFIGNCYS+SP  +I RF  +RS
Sbjct: 56  ENVLENVLQFLTSRCDRNAVSLVCRSWYRVEAQTRLEVFIGNCYSLSPARLIHRFKRVRS 115

Query: 84  VTLKGKPRFSDFNLVPPNWGADIHSWLVAFASKYPILEELRLKRMAVTDESLEFLGRSFP 143
           + LKGKPRF+DFNL+PPNWGA    W+ A A  YP LE++ LKRM VTD+ L  L  SFP
Sbjct: 116 LVLKGKPRFADFNLMPPNWGAQFSPWVAATAKAYPWLEKVHLKRMFVTDDDLALLAESFP 175

Query: 144 NFKALSMMSCDGFSTDGLAAIATHCKNLTELDILENDIIDKSGNWLSCFPDTLKSLEVLN 203
            FK L+++ C+GF T G+A +A  C+ L  LD++E+++ D   +W+SCFP+    LE L+
Sbjct: 176 GFKELTLVCCEGFGTSGIAIVANKCRQLKVLDLMESEVTDDELDWISCFPEGETHLESLS 235

Query: 204 FSSLNSDVSFEALEKLVRRCKSLKVLKVNRNINLEQLQRLLVHTPQLTELGTGSFSQEIT 263
           F  + S ++F+ALE+LV R   LK L+ NR ++LE+L RL+V  PQLT LGTGSFS +  
Sbjct: 236 FDCVESPINFKALEELVVRSPFLKKLRTNRFVSLEELHRLMVRAPQLTSLGTGSFSPDNV 295

Query: 264 LR--QYCDLEDAFKSCKNLRTLSGLLESTVLYLQVLFPACANLTFLNLSYAILHGGELAG 323
            +  Q  D   AF++CK++  LSG  E    YL  +   CANLT LN SYA +    L  
Sbjct: 296 PQGEQQPDYAAAFRACKSIVCLSGFREFRPEYLLAISSVCANLTSLNFSYANISPHMLKP 355

Query: 324 LLSHCPVLHRLWVLDTVEDKGLKAVGLSCPLLEELRVFPADPFADNLVHGVTESGFLAVS 383
           ++S+C  +   W LD++ D+GL+AV  +C  L ELR+FP DP  D+    V+  G  A+S
Sbjct: 356 IISNCHNIRVFWALDSIRDEGLQAVAATCKELRELRIFPFDPREDS-EGPVSGVGLQAIS 415

Query: 384 YGCRKLHYVLYFCRQMTNEAVATIVQNCPDFTHFRLCIMNPHQPDYLTKQPMDEAFGAVV 443
            GCRKL  +LYFC+ MTN AV  + +NCP  T FRLCIM  H+PD++T +PMD+ FGA+V
Sbjct: 416 EGCRKLESILYFCQNMTNGAVTAMSENCPQLTVFRLCIMGRHRPDHVTGKPMDDGFGAIV 475

Query: 444 KTCSRLRRLAISGLLTDLTFEYIGKYAKNLETLSVAFAGSSDWGMQCVMSGCPKLRKLEI 503
           K C +L RLA+SGLLTD  F YIG+Y K + TLSVAFAG+SD  ++ V+ GCPKL+KLEI
Sbjct: 476 KNCKKLTRLAVSGLLTDEAFSYIGEYGKLIRTLSVAFAGNSDKALRYVLEGCPKLQKLEI 535

Query: 504 RDSPFGNAALLSGLERYESMRSLWMSACKVTMNGCRVLAKQVPRLNVEVIKDGGSDECE- 563
           RDSPFG+  L SG+ RY +MR +W+S+C ++  GCR ++  +P + VEV    G D+ + 
Sbjct: 536 RDSPFGDVGLRSGMHRYSNMRFVWLSSCLISRGGCRGVSHALPNVVVEVFGADGDDDEDT 595

Query: 564 -----AESVYVYRSVAGPRRDAPSFVLTL 585
                 E++Y+YRS+ GPR+DAP FV  L
Sbjct: 596 VTGDYVETLYLYRSLDGPRKDAPKFVTIL 623

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038883074.10.0e+0096.75protein TRANSPORT INHIBITOR RESPONSE 1-like [Benincasa hispida][more]
XP_008439908.10.0e+0095.55PREDICTED: protein AUXIN SIGNALING F-BOX 2-like [Cucumis melo] >TYK13102.1 prote... [more]
XP_004134961.10.0e+0095.03protein AUXIN SIGNALING F-BOX 2 [Cucumis sativus] >XP_031743133.1 protein AUXIN ... [more]
KAG7033903.10.0e+0094.37Transport inhibitor response 1-like protein [Cucurbita argyrosperma subsp. argyr... [more]
XP_022977973.10.0e+0094.37transport inhibitor response 1-like protein Os04g0395600 isoform X1 [Cucurbita m... [more]
Match NameE-valueIdentityDescription
Q570C01.5e-18656.14Protein TRANSPORT INHIBITOR RESPONSE 1 OS=Arabidopsis thaliana OX=3702 GN=TIR1 P... [more]
Q9LW291.8e-18253.40Protein AUXIN SIGNALING F-BOX 2 OS=Arabidopsis thaliana OX=3702 GN=AFB2 PE=1 SV=... [more]
Q7XVM81.3e-18054.10Transport inhibitor response 1-like protein Os04g0395600 OS=Oryza sativa subsp. ... [more]
Q9LPW73.7e-18053.62Protein AUXIN SIGNALING F-BOX 3 OS=Arabidopsis thaliana OX=3702 GN=AFB3 PE=1 SV=... [more]
Q0DKP38.7e-16650.79Transport inhibitor response 1-like protein Os05g0150500 OS=Oryza sativa subsp. ... [more]
Match NameE-valueIdentityDescription
A0A5D3CP230.0e+0095.55Protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3B0L80.0e+0095.55protein AUXIN SIGNALING F-BOX 2-like OS=Cucumis melo OX=3656 GN=LOC103484554 PE=... [more]
A0A0A0KN740.0e+0095.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G516940 PE=4 SV=1[more]
A0A6J1ISU20.0e+0094.37transport inhibitor response 1-like protein Os04g0395600 isoform X1 OS=Cucurbita... [more]
A0A6J1GCV40.0e+0094.37transport inhibitor response 1-like protein Os04g0395600 isoform X1 OS=Cucurbita... [more]
Match NameE-valueIdentityDescription
AT3G62980.11.1e-18756.14F-box/RNI-like superfamily protein [more]
AT3G26810.11.2e-18353.40auxin signaling F-box 2 [more]
AT1G12820.12.6e-18153.62auxin signaling F-box 3 [more]
AT4G03190.11.8e-16650.97GRR1-like protein 1 [more]
AT4G24390.29.9e-16549.38RNI-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006553Leucine-rich repeat, cysteine-containing subtypeSMARTSM00367LRR_CC_2coord: 117..141
e-value: 590.0
score: 0.7
coord: 142..167
e-value: 120.0
score: 6.3
coord: 384..408
e-value: 19.0
score: 12.4
coord: 493..517
e-value: 340.0
score: 2.6
coord: 444..467
e-value: 46.0
score: 9.3
IPR041101Transport inhibitor response 1 domainPFAMPF18791Transp_inhibitcoord: 79..125
e-value: 1.4E-22
score: 78.9
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 59..584
e-value: 1.7E-169
score: 566.5
NoneNo IPR availableGENE3D1.20.1280.50coord: 16..58
e-value: 2.6E-16
score: 60.8
NoneNo IPR availablePANTHERPTHR16134:SF37PROTEIN AUXIN SIGNALING F-BOX 3-LIKEcoord: 15..583
NoneNo IPR availablePANTHERPTHR16134F-BOX/TPR REPEAT PROTEIN POF3coord: 15..583
NoneNo IPR availableSUPERFAMILY52047RNI-likecoord: 36..547
IPR041567COI1, F-boxPFAMPF18511F-box_5coord: 21..60
e-value: 8.0E-20
score: 70.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10016122.1HG10016122.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009734 auxin-activated signaling pathway
biological_process GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
cellular_component GO:0019005 SCF ubiquitin ligase complex