HG10015995 (gene) Bottle gourd (Hangzhou Gourd) v1

Overview
NameHG10015995
Typegene
OrganismLagenaria siceraria cv. Hangzhou Gourd (Bottle gourd (Hangzhou Gourd) v1)
DescriptionCBL-interacting serine/threonine-protein kinase 12
LocationChr03: 2048293 .. 2049696 (+)
RNA-Seq ExpressionHG10015995
SyntenyHG10015995
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGACCGGCCGGACCCTGACAAGAAGACCGAAACCACTAGGAAGGACATGCCCGCGCTTCTTTTGGGCCGATACGAGATCGGGAAGCTCCTTGGCCATGGGACCTTCGCTAAGGTCTACCATGCCCGAAACATCAAGACCAACGAAAGCGTCGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTTTAATCGCCCACATCAAGCGCGAGATCTCAATCCTCCGACGTGTTCGACACCCCAATATTGTGCAACTCTTCGAGGTCATGGCCACCAAGGCCAAGATCTACTTCGTTATGGAATACGTCCGCGGTGGCGAGCTTTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCACGAAAGTACTTTCAGCAATTAATCTCCGCCGTTGCTTTTTGCCACGCGCGCGGTGTTTATCATCGCGACCTTAAACCGGAGAATTTGCTGCTCGATGAGAATGGGAATCTTAAGGTCTCCGATTTTGGCCTCAGCGCCGTCTCCGATCAAATTCGACAAGATGGGTTGTTTCATACTTTTTGTGGTACACCGGCGTATGTGGCTCCGGAGGTTTTGGCCCGGAAAGGGTATGAGGCTGCGAAGGTCGATATTTGGTCATGTGGGGTTATTCTGTTTGTTCTGATGGCGGGTTATTTGCCCTTCCACGACCAAAACATTATGGCAATGTATAAGAAGATTTACAAGGGGGAGTTTCGGTGTCCCAGATGGTTCTCGCCGGAGCTAATTCGACTCCTAACTCGTCTTTTAGATACAAATCCCGAAACCCGTTTCACAATTCCTGAAATCATGGAGAATAGATGGTTCAAAAAGGGGTATAAGCATATCAAATTCTACATCGAAGACGATAAGGTCTGCAGCGTTGAGGATGACAACGATGATGTCGATTCCTTGTCGGATCAATCACAATCGGAATCGGATTCCGAAATTATTGAGACCAGAAGAAAGGTTACCTCACTCCCAAGACCCGCGAGTTTGAATGCATTTGATATAATTTCATTTTCGCCCGGATTTGATCTTTCTGGATTGTTTGAGGATGGAGGGGAAGAGGCAAGATTCGTGTCCAATGCCCCGGTGTCGAAAATTATATCGAAATTGGAGGAGATTGCAAAATTGGTGAGTTTTACTGTGAGGAAGAAGGATTGCAGGGTTAGTTTAGAGGGGTCACGGGAAGGAGTGAAAGGGCCATTGACAATCGCGGCCGAGGTATTTGAGTTGACGCCGAATTTGGTGATGGTGGAGGTGAAGAGGAAAGGGGGAGATAAAGCAGAGTATGAACAGTTCTGTAATAATGAATTGAAGCCAGCATTGTTGAATCTGAAGGTGGAAGACTCTGCTGCTCCATCACATATACCATCACTTACTGCATGA

mRNA sequence

ATGGCCGACCGGCCGGACCCTGACAAGAAGACCGAAACCACTAGGAAGGACATGCCCGCGCTTCTTTTGGGCCGATACGAGATCGGGAAGCTCCTTGGCCATGGGACCTTCGCTAAGGTCTACCATGCCCGAAACATCAAGACCAACGAAAGCGTCGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTTTAATCGCCCACATCAAGCGCGAGATCTCAATCCTCCGACGTGTTCGACACCCCAATATTGTGCAACTCTTCGAGGTCATGGCCACCAAGGCCAAGATCTACTTCGTTATGGAATACGTCCGCGGTGGCGAGCTTTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCACGAAAGTACTTTCAGCAATTAATCTCCGCCGTTGCTTTTTGCCACGCGCGCGGTGTTTATCATCGCGACCTTAAACCGGAGAATTTGCTGCTCGATGAGAATGGGAATCTTAAGGTCTCCGATTTTGGCCTCAGCGCCGTCTCCGATCAAATTCGACAAGATGGGTTGTTTCATACTTTTTGTGGTACACCGGCGTATGTGGCTCCGGAGGTTTTGGCCCGGAAAGGGTATGAGGCTGCGAAGGTCGATATTTGGTCATGTGGGGTTATTCTGTTTGTTCTGATGGCGGGTTATTTGCCCTTCCACGACCAAAACATTATGGCAATGTATAAGAAGATTTACAAGGGGGAGTTTCGGTGTCCCAGATGGTTCTCGCCGGAGCTAATTCGACTCCTAACTCGTCTTTTAGATACAAATCCCGAAACCCGTTTCACAATTCCTGAAATCATGGAGAATAGATGGTTCAAAAAGGGGTATAAGCATATCAAATTCTACATCGAAGACGATAAGGTCTGCAGCGTTGAGGATGACAACGATGATGTCGATTCCTTGTCGGATCAATCACAATCGGAATCGGATTCCGAAATTATTGAGACCAGAAGAAAGGTTACCTCACTCCCAAGACCCGCGAGTTTGAATGCATTTGATATAATTTCATTTTCGCCCGGATTTGATCTTTCTGGATTGTTTGAGGATGGAGGGGAAGAGGCAAGATTCGTGTCCAATGCCCCGGTGTCGAAAATTATATCGAAATTGGAGGAGATTGCAAAATTGGTGAGTTTTACTGTGAGGAAGAAGGATTGCAGGGTTAGTTTAGAGGGGTCACGGGAAGGAGTGAAAGGGCCATTGACAATCGCGGCCGAGGTATTTGAGTTGACGCCGAATTTGGTGATGGTGGAGGTGAAGAGGAAAGGGGGAGATAAAGCAGAGTATGAACAGTTCTGTAATAATGAATTGAAGCCAGCATTGTTGAATCTGAAGGTGGAAGACTCTGCTGCTCCATCACATATACCATCACTTACTGCATGA

Coding sequence (CDS)

ATGGCCGACCGGCCGGACCCTGACAAGAAGACCGAAACCACTAGGAAGGACATGCCCGCGCTTCTTTTGGGCCGATACGAGATCGGGAAGCTCCTTGGCCATGGGACCTTCGCTAAGGTCTACCATGCCCGAAACATCAAGACCAACGAAAGCGTCGCCATTAAAGTCATCGACAAGGAGAAGATCCTCAAGGGCGGTTTAATCGCCCACATCAAGCGCGAGATCTCAATCCTCCGACGTGTTCGACACCCCAATATTGTGCAACTCTTCGAGGTCATGGCCACCAAGGCCAAGATCTACTTCGTTATGGAATACGTCCGCGGTGGCGAGCTTTTCAAGAAGGTCTCCAAGGGCCGATTGAAGGAAGAGGTCGCACGAAAGTACTTTCAGCAATTAATCTCCGCCGTTGCTTTTTGCCACGCGCGCGGTGTTTATCATCGCGACCTTAAACCGGAGAATTTGCTGCTCGATGAGAATGGGAATCTTAAGGTCTCCGATTTTGGCCTCAGCGCCGTCTCCGATCAAATTCGACAAGATGGGTTGTTTCATACTTTTTGTGGTACACCGGCGTATGTGGCTCCGGAGGTTTTGGCCCGGAAAGGGTATGAGGCTGCGAAGGTCGATATTTGGTCATGTGGGGTTATTCTGTTTGTTCTGATGGCGGGTTATTTGCCCTTCCACGACCAAAACATTATGGCAATGTATAAGAAGATTTACAAGGGGGAGTTTCGGTGTCCCAGATGGTTCTCGCCGGAGCTAATTCGACTCCTAACTCGTCTTTTAGATACAAATCCCGAAACCCGTTTCACAATTCCTGAAATCATGGAGAATAGATGGTTCAAAAAGGGGTATAAGCATATCAAATTCTACATCGAAGACGATAAGGTCTGCAGCGTTGAGGATGACAACGATGATGTCGATTCCTTGTCGGATCAATCACAATCGGAATCGGATTCCGAAATTATTGAGACCAGAAGAAAGGTTACCTCACTCCCAAGACCCGCGAGTTTGAATGCATTTGATATAATTTCATTTTCGCCCGGATTTGATCTTTCTGGATTGTTTGAGGATGGAGGGGAAGAGGCAAGATTCGTGTCCAATGCCCCGGTGTCGAAAATTATATCGAAATTGGAGGAGATTGCAAAATTGGTGAGTTTTACTGTGAGGAAGAAGGATTGCAGGGTTAGTTTAGAGGGGTCACGGGAAGGAGTGAAAGGGCCATTGACAATCGCGGCCGAGGTATTTGAGTTGACGCCGAATTTGGTGATGGTGGAGGTGAAGAGGAAAGGGGGAGATAAAGCAGAGTATGAACAGTTCTGTAATAATGAATTGAAGCCAGCATTGTTGAATCTGAAGGTGGAAGACTCTGCTGCTCCATCACATATACCATCACTTACTGCATGA

Protein sequence

MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLTA
Homology
BLAST of HG10015995 vs. NCBI nr
Match: XP_008439731.1 (PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo] >KAA0052614.1 CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo var. makuwa] >TYK13214.1 CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo var. makuwa])

HSP 1 Score: 916.4 bits (2367), Expect = 1.0e-262
Identity = 461/466 (98.93%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPN 420
           EARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTP 
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDS  PSHIPS T
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDT 466

BLAST of HG10015995 vs. NCBI nr
Match: XP_038882513.1 (CBL-interacting serine/threonine-protein kinase 12-like [Benincasa hispida])

HSP 1 Score: 915.6 bits (2365), Expect = 1.7e-262
Identity = 460/466 (98.71%), Postives = 463/466 (99.36%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDN+DVDSLSDQSQSESDSEI ETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNNDVDSLSDQSQSESDSEIFETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPN 420
           EARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGV+GPLTIAAEVFELTP 
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVRGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPS T
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSDT 466

BLAST of HG10015995 vs. NCBI nr
Match: XP_011658253.1 (CBL-interacting serine/threonine-protein kinase 12 [Cucumis sativus] >KGN49312.1 hypothetical protein Csa_003581 [Cucumis sativus])

HSP 1 Score: 910.6 bits (2352), Expect = 5.5e-261
Identity = 457/466 (98.07%), Postives = 460/466 (98.71%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTN+SVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNDSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DD DDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPN 420
           EARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTP 
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           LVMVEVKRKGGDK EYEQFCNNELKPALLNLKVEDS  PSHIPS T
Sbjct: 421 LVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDT 466

BLAST of HG10015995 vs. NCBI nr
Match: XP_022926641.1 (CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita moschata] >KAG6594541.1 CBL-interacting serine/threonine-protein kinase 12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026520.1 CBL-interacting serine/threonine-protein kinase 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 874.0 bits (2257), Expect = 5.7e-250
Identity = 447/473 (94.50%), Postives = 457/473 (96.62%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDV---DSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQSQSESDSEIIETRRKV++LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLK--VEDSAA--PSHIPSLT 467
           T  LVMVEVKRKGGDKAEYEQFCN ELKPALLNLK  VEDSAA   SHIPS T
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDT 473

BLAST of HG10015995 vs. NCBI nr
Match: XP_023518648.1 (CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 873.6 bits (2256), Expect = 7.5e-250
Identity = 446/473 (94.29%), Postives = 457/473 (96.62%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDV---DSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQSQSESDSEIIETRRKV++LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLK--VEDSAA--PSHIPSLT 467
           T  LVMVEVKRKGGDKAEYEQFCN ELKPALLNLK  VEDSAA   SHIPS T
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDT 473

BLAST of HG10015995 vs. ExPASy Swiss-Prot
Match: Q9SN43 (CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=3702 GN=CIPK12 PE=1 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 3.2e-219
Identity = 380/462 (82.25%), Postives = 414/462 (89.61%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MA++   +      R    AL+LGRYE+GKLLGHGTFAKVY ARN+KTNESVAIKVIDKE
Sbjct: 1   MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           K+LKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELF KV+KGRL
Sbjct: 61  KVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGY+AAKVDIWSCGVILFVLMAGYLPFHD+N+MAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFS EL RLL++LL+TNPE RFT PEIMEN WFKKG+KHIKFY+EDDK+C+V 
Sbjct: 241 GEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVV 300

Query: 301 DD----NDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFE 360
           DD    +D V+S  D + SES+ E +E RR+V  LPRPASLNAFDIISFS GFDLSGLF+
Sbjct: 301 DDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD 360

Query: 361 DGGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFE 420
           D GE +RFVS APVSKIISKLEEIAK+VSFTVRKKDCRVSLEGSR+GVKGPLTIAAE+FE
Sbjct: 361 DDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFE 420

Query: 421 LTPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAA 459
           LTP+LV+VEVK+KGGDK EYE FCNNELKP L NL  +D  A
Sbjct: 421 LTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVA 462

BLAST of HG10015995 vs. ExPASy Swiss-Prot
Match: Q9FJ55 (CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana OX=3702 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 5.1e-201
Identity = 351/463 (75.81%), Postives = 407/463 (87.90%), Query Frame = 0

Query: 10  KTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIA 69
           K +  + +  AL+LG+YE+G+LLGHGTFAKVY ARN ++ ESVAIKVIDKEK+LK GLIA
Sbjct: 12  KKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIA 71

Query: 70  HIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYF 129
           HIKREISILRRVRHPNIVQLFEVMATK+KIYFVMEYV+GGELF KV+KGRLKEE+ARKYF
Sbjct: 72  HIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYF 131

Query: 130 QQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 189
           QQLISAV+FCH RGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP
Sbjct: 132 QQLISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 191

Query: 190 AYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWF 249
           AYVAPEVLARKGY+ AKVDIWSCGVILFVLMAG+LPFHD+N+MAMYKKIY+G+FRCPRWF
Sbjct: 192 AYVAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWF 251

Query: 250 SPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDD-KVCSVEDDN--DDV 309
             E+ RLL R+L+T PE RFT+P+IME  WFKKG+KHIKFY+EDD ++C+V DD+  + +
Sbjct: 252 PVEINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESI 311

Query: 310 DSLSDQSQSESDSEIIET---RRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEAR 369
           +S+S +S + S+ E  E+   RR+  S+PRPASLNAFD+ISFSPGFDLSGLFED GE +R
Sbjct: 312 ESVSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGSR 371

Query: 370 FVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREG-VKGPLTIAAEVFELTPNLV 429
           FVS APV +IISKLEEIA++VSFTVRKKDC+VSLEGSREG +KGPL+IAAE+FELTP LV
Sbjct: 372 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 431

Query: 430 MVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSL 466
           +VEVK+KGGDK EY++FCN ELKP L NL  E+    S   SL
Sbjct: 432 VVEVKKKGGDKMEYDEFCNKELKPKLQNLSSENGQRVSGSRSL 474

BLAST of HG10015995 vs. ExPASy Swiss-Prot
Match: Q9LP51 (CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana OX=3702 GN=CIPK18 PE=1 SV=1)

HSP 1 Score: 677.2 bits (1746), Expect = 1.4e-193
Identity = 332/438 (75.80%), Postives = 384/438 (87.67%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+G+YE+GKLLGHGTFAKVY A+NIK+ + VAIKVIDKEKI+K GL+AHIKREISILRR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHP IV LFEVMATK+KIYFVMEYV GGELF  V+KGRL EE AR+YFQQLIS+V+FCH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
            RGVYHRDLKPENLLLD  GNLKVSDFGLSAV++Q+RQDGL HTFCGTPAY+APEVL RK
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+AAK D+WSCGVILFVLMAG++PF+D+NIM MYKKIYKGEFRCPRWFS +L+RLLTRL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQSQ--SESD 320
           LDTNP+TR TIPEIM+NRWFKKG+KH+KFYIEDDK+C  ++D ++  S S +S   SESD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 321 SEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLF-EDGGEEARFVSNAPVSKIISKL 380
           +E    R  + S+PRP+SLNAFDIISFS GFDLSGLF E+GGE  RFVS APVSKIISKL
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEEEGGEGTRFVSGAPVSKIISKL 428

Query: 381 EEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPNLVMVEVKRKGGDKAEYE 440
           EEIAK+VSFTVRKK+  + LEG REG KGPLTIAAE+FELTP+LV+VEVK+KGGD+ EYE
Sbjct: 429 EEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDREEYE 488

Query: 441 QFCNNELKPALLNLKVED 456
           +FCN EL+P L  L  E+
Sbjct: 489 EFCNKELRPELEKLIHEE 506

BLAST of HG10015995 vs. ExPASy Swiss-Prot
Match: O22971 (CBL-interacting serine/threonine-protein kinase 13 OS=Arabidopsis thaliana OX=3702 GN=CIPK13 PE=1 SV=2)

HSP 1 Score: 630.9 bits (1626), Expect = 1.1e-179
Identity = 314/442 (71.04%), Postives = 368/442 (83.26%), Query Frame = 0

Query: 15  RKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKRE 74
           R    ++L+ +YEIGKLLGHG+FAKVY ARNI + E VAIKVIDKEKI+K GL  HIKRE
Sbjct: 46  RTPQGSILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKRE 105

Query: 75  ISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLIS 134
           ISILRRVRHP IV L EVMATK KIY VMEYVRGGEL+  V++GRL+E  AR+YFQQLIS
Sbjct: 106 ISILRRVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLIS 165

Query: 135 AVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAP 194
           +VAFCH+RGVYHRDLK ENLLLD+ GN+KVSDFGLS VS+Q++Q+G+  TFCGTPAY+AP
Sbjct: 166 SVAFCHSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAP 225

Query: 195 EVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELI 254
           EVL RKGYE AK DIWSCGVILFVLMAGYLPF D+NI+ MY KIYKG+F+CP+WFSPEL 
Sbjct: 226 EVLTRKGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELA 285

Query: 255 RLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLS---- 314
           RL+TR+LDTNP+TR TIPEIM++RWFKKG+KH+KFYIE+DK+C  +DDNDD DS S    
Sbjct: 286 RLVTRMLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSG 345

Query: 315 -DQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSNAP 374
              + SE D+E     ++V S+PRPASLNAFDI+SFS   DLSGLFE+GG+ ARFVS AP
Sbjct: 346 RSSTASEGDAEF--DIKRVDSMPRPASLNAFDILSFS---DLSGLFEEGGQGARFVSAAP 405

Query: 375 VSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPNLVMVEVKRK 434
           ++KIISKLEEIAK V F VRKKD  V LEG REG KGPLTI  E+FELTP+LV+VEVK+K
Sbjct: 406 MTKIISKLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKK 465

Query: 435 GGDKAEYEQFCNNELKPALLNL 452
           GG+  EYE+FCN EL+P L  L
Sbjct: 466 GGNIEEYEEFCNKELRPQLEKL 482

BLAST of HG10015995 vs. ExPASy Swiss-Prot
Match: Q68Y49 (CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK19 PE=1 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 5.5e-179
Identity = 313/477 (65.62%), Postives = 380/477 (79.66%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           LLLGRYE+G+LLGHGTFAKVY AR+  + E VAIKV+DKEK ++ GL+ HIKREI+ILRR
Sbjct: 32  LLLGRYELGRLLGHGTFAKVYQARSADSGEPVAIKVLDKEKAMRHGLVPHIKREIAILRR 91

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHPNIV+LFEVMATK+KIYFVME VRGGELF +V+KGRLKE+ AR+YFQQL+SAV FCH
Sbjct: 92  VRHPNIVRLFEVMATKSKIYFVMELVRGGELFGRVAKGRLKEDTARRYFQQLVSAVGFCH 151

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
           ARGV+HRDLKPENLL+DE+G+LKVSDFGLSAV+DQ   DGL HTFCGTP+YVAPEVLAR+
Sbjct: 152 ARGVFHRDLKPENLLVDEHGDLKVSDFGLSAVADQFHPDGLLHTFCGTPSYVAPEVLARR 211

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+ AK DIWSCG+ILFVLMAGYLPFHDQN+MAMY+KIY+GEFRCPRWFS +L  LL R+
Sbjct: 212 GYDGAKADIWSCGIILFVLMAGYLPFHDQNLMAMYRKIYRGEFRCPRWFSKDLSSLLNRI 271

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSL----------- 320
           LDTNPETR T+ E+ME+RWF+KG++ ++FY+EDD+V S+ D ++D+  L           
Sbjct: 272 LDTNPETRITVKEVMESRWFQKGFRPVRFYVEDDQVHSLADGDNDMPELEPSEPPPPPPF 331

Query: 321 ----------SDQSQSESDSEII----------ETRRKVTSLPRPASLNAFDIISFSPGF 380
                      ++S  ESDS +             +R + SL RPASLNAFDIISFS GF
Sbjct: 332 PPPPPQQDDDGEESGWESDSSVASCPATLSSEERRQRPLGSLTRPASLNAFDIISFSKGF 391

Query: 381 DLSGLFEDGGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLT 440
           DLSGLFE+ G E RF+S  P+  II+KLEEIAK+ SF VR+KD RVS+EG+REG+KGPLT
Sbjct: 392 DLSGLFEERGSEVRFISAEPMQTIITKLEEIAKVKSFFVRRKDWRVSIEGTREGLKGPLT 451

Query: 441 IAAEVFELTPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           I AE+FELTP+LV+VEVK+K GDK EY+ FCN ELKP + +L V    +  +IPS T
Sbjct: 452 IGAEIFELTPSLVVVEVKKKAGDKEEYDDFCNRELKPGMQHL-VHHMGSVPNIPSDT 507

BLAST of HG10015995 vs. ExPASy TrEMBL
Match: A0A5A7UB69 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008270 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 4.9e-263
Identity = 461/466 (98.93%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPN 420
           EARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTP 
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDS  PSHIPS T
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDT 466

BLAST of HG10015995 vs. ExPASy TrEMBL
Match: A0A1S3AZF1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103484445 PE=3 SV=1)

HSP 1 Score: 916.4 bits (2367), Expect = 4.9e-263
Identity = 461/466 (98.93%), Postives = 462/466 (99.14%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPN 420
           EARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTP 
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDS  PSHIPS T
Sbjct: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSGDPSHIPSDT 466

BLAST of HG10015995 vs. ExPASy TrEMBL
Match: A0A0A0KLP8 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6G519640 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.7e-261
Identity = 457/466 (98.07%), Postives = 460/466 (98.71%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTN+SVAIKVIDKE
Sbjct: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNDSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360
           DD DDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE
Sbjct: 301 DDTDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE 360

Query: 361 EARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPN 420
           EARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTP 
Sbjct: 361 EARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPK 420

Query: 421 LVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSLT 467
           LVMVEVKRKGGDK EYEQFCNNELKPALLNLKVEDS  PSHIPS T
Sbjct: 421 LVMVEVKRKGGDKEEYEQFCNNELKPALLNLKVEDSGDPSHIPSDT 466

BLAST of HG10015995 vs. ExPASy TrEMBL
Match: A0A6J1EFG7 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111433713 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.8e-250
Identity = 447/473 (94.50%), Postives = 457/473 (96.62%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300

Query: 301 DDNDDV---DSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQSQSESDSEIIETRRKV++LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLK--VEDSAA--PSHIPSLT 467
           T  LVMVEVKRKGGDKAEYEQFCN ELKPALLNLK  VEDSAA   SHIPS T
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDT 473

BLAST of HG10015995 vs. ExPASy TrEMBL
Match: A0A6J1KSW8 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111496943 PE=3 SV=1)

HSP 1 Score: 871.3 bits (2250), Expect = 1.8e-249
Identity = 445/473 (94.08%), Postives = 456/473 (96.41%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MADRPD DKKTETT+KDMPALLLGRYEIGKLLGHGTFAKVYHARNIKT++SVAIKVIDKE
Sbjct: 1   MADRPDTDKKTETTKKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTDQSVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL
Sbjct: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAVAFCHARGV+HRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVAFCHARGVFHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVL RKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLTRKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFSPELIRLL+RLLDTNPETRFTIPEI ENRWFKKGYKHIKFYIEDDKVCS+E
Sbjct: 241 GEFRCPRWFSPELIRLLSRLLDTNPETRFTIPEITENRWFKKGYKHIKFYIEDDKVCSIE 300

Query: 301 DDNDDV---DSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFED 360
           DD +DV   DS SDQSQSESDSEIIETRRKV++LPRPASLNAFDIISFSPGFDLSGLFED
Sbjct: 301 DDQNDVDIIDSSSDQSQSESDSEIIETRRKVSTLPRPASLNAFDIISFSPGFDLSGLFED 360

Query: 361 GGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420
           GGEEARFVS+APVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL
Sbjct: 361 GGEEARFVSSAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFEL 420

Query: 421 TPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLK--VEDSAA--PSHIPSLT 467
           T  LVMVEVKRKGGDKAEYEQFCN ELKPALLNLK  VEDSAA   SHIPS T
Sbjct: 421 TQKLVMVEVKRKGGDKAEYEQFCNEELKPALLNLKVEVEDSAAAPSSHIPSDT 473

BLAST of HG10015995 vs. TAIR 10
Match: AT4G18700.1 (CBL-interacting protein kinase 12 )

HSP 1 Score: 762.3 bits (1967), Expect = 2.3e-220
Identity = 380/462 (82.25%), Postives = 414/462 (89.61%), Query Frame = 0

Query: 1   MADRPDPDKKTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKE 60
           MA++   +      R    AL+LGRYE+GKLLGHGTFAKVY ARN+KTNESVAIKVIDKE
Sbjct: 1   MAEKITRETSLPKERSSPQALILGRYEMGKLLGHGTFAKVYLARNVKTNESVAIKVIDKE 60

Query: 61  KILKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRL 120
           K+LKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELF KV+KGRL
Sbjct: 61  KVLKGGLIAHIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFNKVAKGRL 120

Query: 121 KEEVARKYFQQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180
           KEEVARKYFQQLISAV FCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG
Sbjct: 121 KEEVARKYFQQLISAVTFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDG 180

Query: 181 LFHTFCGTPAYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYK 240
           LFHTFCGTPAYVAPEVLARKGY+AAKVDIWSCGVILFVLMAGYLPFHD+N+MAMYKKIY+
Sbjct: 181 LFHTFCGTPAYVAPEVLARKGYDAAKVDIWSCGVILFVLMAGYLPFHDRNVMAMYKKIYR 240

Query: 241 GEFRCPRWFSPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVE 300
           GEFRCPRWFS EL RLL++LL+TNPE RFT PEIMEN WFKKG+KHIKFY+EDDK+C+V 
Sbjct: 241 GEFRCPRWFSTELTRLLSKLLETNPEKRFTFPEIMENSWFKKGFKHIKFYVEDDKLCNVV 300

Query: 301 DD----NDDVDSLSDQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFE 360
           DD    +D V+S  D + SES+ E +E RR+V  LPRPASLNAFDIISFS GFDLSGLF+
Sbjct: 301 DDDELESDSVESDRDSAASESEIEYLEPRRRVGGLPRPASLNAFDIISFSQGFDLSGLFD 360

Query: 361 DGGEEARFVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFE 420
           D GE +RFVS APVSKIISKLEEIAK+VSFTVRKKDCRVSLEGSR+GVKGPLTIAAE+FE
Sbjct: 361 DDGEGSRFVSGAPVSKIISKLEEIAKVVSFTVRKKDCRVSLEGSRQGVKGPLTIAAEIFE 420

Query: 421 LTPNLVMVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAA 459
           LTP+LV+VEVK+KGGDK EYE FCNNELKP L NL  +D  A
Sbjct: 421 LTPSLVVVEVKKKGGDKTEYEDFCNNELKPKLQNLTADDVVA 462

BLAST of HG10015995 vs. TAIR 10
Match: AT5G45810.1 (CBL-interacting protein kinase 19 )

HSP 1 Score: 701.8 bits (1810), Expect = 3.6e-202
Identity = 351/463 (75.81%), Postives = 407/463 (87.90%), Query Frame = 0

Query: 10  KTETTRKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIA 69
           K +  + +  AL+LG+YE+G+LLGHGTFAKVY ARN ++ ESVAIKVIDKEK+LK GLIA
Sbjct: 12  KKKQDQSNHQALILGKYEMGRLLGHGTFAKVYLARNAQSGESVAIKVIDKEKVLKSGLIA 71

Query: 70  HIKREISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYF 129
           HIKREISILRRVRHPNIVQLFEVMATK+KIYFVMEYV+GGELF KV+KGRLKEE+ARKYF
Sbjct: 72  HIKREISILRRVRHPNIVQLFEVMATKSKIYFVMEYVKGGELFNKVAKGRLKEEMARKYF 131

Query: 130 QQLISAVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 189
           QQLISAV+FCH RGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP
Sbjct: 132 QQLISAVSFCHFRGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTP 191

Query: 190 AYVAPEVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWF 249
           AYVAPEVLARKGY+ AKVDIWSCGVILFVLMAG+LPFHD+N+MAMYKKIY+G+FRCPRWF
Sbjct: 192 AYVAPEVLARKGYDGAKVDIWSCGVILFVLMAGFLPFHDRNVMAMYKKIYRGDFRCPRWF 251

Query: 250 SPELIRLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDD-KVCSVEDDN--DDV 309
             E+ RLL R+L+T PE RFT+P+IME  WFKKG+KHIKFY+EDD ++C+V DD+  + +
Sbjct: 252 PVEINRLLIRMLETKPERRFTMPDIMETSWFKKGFKHIKFYVEDDHQLCNVADDDEIESI 311

Query: 310 DSLSDQSQSESDSEIIET---RRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEAR 369
           +S+S +S + S+ E  E+   RR+  S+PRPASLNAFD+ISFSPGFDLSGLFED GE +R
Sbjct: 312 ESVSGRSSTVSEPEDFESFDGRRRGGSMPRPASLNAFDLISFSPGFDLSGLFEDDGEGSR 371

Query: 370 FVSNAPVSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREG-VKGPLTIAAEVFELTPNLV 429
           FVS APV +IISKLEEIA++VSFTVRKKDC+VSLEGSREG +KGPL+IAAE+FELTP LV
Sbjct: 372 FVSGAPVGQIISKLEEIARIVSFTVRKKDCKVSLEGSREGSMKGPLSIAAEIFELTPALV 431

Query: 430 MVEVKRKGGDKAEYEQFCNNELKPALLNLKVEDSAAPSHIPSL 466
           +VEVK+KGGDK EY++FCN ELKP L NL  E+    S   SL
Sbjct: 432 VVEVKKKGGDKMEYDEFCNKELKPKLQNLSSENGQRVSGSRSL 474

BLAST of HG10015995 vs. TAIR 10
Match: AT1G29230.1 (CBL-interacting protein kinase 18 )

HSP 1 Score: 677.2 bits (1746), Expect = 9.6e-195
Identity = 332/438 (75.80%), Postives = 384/438 (87.67%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           +L+G+YE+GKLLGHGTFAKVY A+NIK+ + VAIKVIDKEKI+K GL+AHIKREISILRR
Sbjct: 69  ILMGKYELGKLLGHGTFAKVYLAQNIKSGDKVAIKVIDKEKIMKSGLVAHIKREISILRR 128

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           VRHP IV LFEVMATK+KIYFVMEYV GGELF  V+KGRL EE AR+YFQQLIS+V+FCH
Sbjct: 129 VRHPYIVHLFEVMATKSKIYFVMEYVGGGELFNTVAKGRLPEETARRYFQQLISSVSFCH 188

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
            RGVYHRDLKPENLLLD  GNLKVSDFGLSAV++Q+RQDGL HTFCGTPAY+APEVL RK
Sbjct: 189 GRGVYHRDLKPENLLLDNKGNLKVSDFGLSAVAEQLRQDGLCHTFCGTPAYIAPEVLTRK 248

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+AAK D+WSCGVILFVLMAG++PF+D+NIM MYKKIYKGEFRCPRWFS +L+RLLTRL
Sbjct: 249 GYDAAKADVWSCGVILFVLMAGHIPFYDKNIMVMYKKIYKGEFRCPRWFSSDLVRLLTRL 308

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQSQ--SESD 320
           LDTNP+TR TIPEIM+NRWFKKG+KH+KFYIEDDK+C  ++D ++  S S +S   SESD
Sbjct: 309 LDTNPDTRITIPEIMKNRWFKKGFKHVKFYIEDDKLCREDEDEEEEASSSGRSSTVSESD 368

Query: 321 SEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLF-EDGGEEARFVSNAPVSKIISKL 380
           +E    R  + S+PRP+SLNAFDIISFS GFDLSGLF E+GGE  RFVS APVSKIISKL
Sbjct: 369 AEFDVKRMGIGSMPRPSSLNAFDIISFSSGFDLSGLFEEEGGEGTRFVSGAPVSKIISKL 428

Query: 381 EEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPNLVMVEVKRKGGDKAEYE 440
           EEIAK+VSFTVRKK+  + LEG REG KGPLTIAAE+FELTP+LV+VEVK+KGGD+ EYE
Sbjct: 429 EEIAKIVSFTVRKKEWSLRLEGCREGAKGPLTIAAEIFELTPSLVVVEVKKKGGDREEYE 488

Query: 441 QFCNNELKPALLNLKVED 456
           +FCN EL+P L  L  E+
Sbjct: 489 EFCNKELRPELEKLIHEE 506

BLAST of HG10015995 vs. TAIR 10
Match: AT2G34180.1 (CBL-interacting protein kinase 13 )

HSP 1 Score: 630.9 bits (1626), Expect = 7.9e-181
Identity = 314/442 (71.04%), Postives = 368/442 (83.26%), Query Frame = 0

Query: 15  RKDMPALLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKRE 74
           R    ++L+ +YEIGKLLGHG+FAKVY ARNI + E VAIKVIDKEKI+K GL  HIKRE
Sbjct: 46  RTPQGSILMDKYEIGKLLGHGSFAKVYLARNIHSGEDVAIKVIDKEKIVKSGLAGHIKRE 105

Query: 75  ISILRRVRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLIS 134
           ISILRRVRHP IV L EVMATK KIY VMEYVRGGEL+  V++GRL+E  AR+YFQQLIS
Sbjct: 106 ISILRRVRHPYIVHLLEVMATKTKIYIVMEYVRGGELYNTVARGRLREGTARRYFQQLIS 165

Query: 135 AVAFCHARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAP 194
           +VAFCH+RGVYHRDLK ENLLLD+ GN+KVSDFGLS VS+Q++Q+G+  TFCGTPAY+AP
Sbjct: 166 SVAFCHSRGVYHRDLKLENLLLDDKGNVKVSDFGLSVVSEQLKQEGICQTFCGTPAYLAP 225

Query: 195 EVLARKGYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELI 254
           EVL RKGYE AK DIWSCGVILFVLMAGYLPF D+NI+ MY KIYKG+F+CP+WFSPEL 
Sbjct: 226 EVLTRKGYEGAKADIWSCGVILFVLMAGYLPFDDKNILVMYTKIYKGQFKCPKWFSPELA 285

Query: 255 RLLTRLLDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLS---- 314
           RL+TR+LDTNP+TR TIPEIM++RWFKKG+KH+KFYIE+DK+C  +DDNDD DS S    
Sbjct: 286 RLVTRMLDTNPDTRITIPEIMKHRWFKKGFKHVKFYIENDKLCREDDDNDDDDSSSLSSG 345

Query: 315 -DQSQSESDSEIIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGEEARFVSNAP 374
              + SE D+E     ++V S+PRPASLNAFDI+SFS   DLSGLFE+GG+ ARFVS AP
Sbjct: 346 RSSTASEGDAEF--DIKRVDSMPRPASLNAFDILSFS---DLSGLFEEGGQGARFVSAAP 405

Query: 375 VSKIISKLEEIAKLVSFTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPNLVMVEVKRK 434
           ++KIISKLEEIAK V F VRKKD  V LEG REG KGPLTI  E+FELTP+LV+VEVK+K
Sbjct: 406 MTKIISKLEEIAKEVKFMVRKKDWSVRLEGCREGAKGPLTIRVEIFELTPSLVVVEVKKK 465

Query: 435 GGDKAEYEQFCNNELKPALLNL 452
           GG+  EYE+FCN EL+P L  L
Sbjct: 466 GGNIEEYEEFCNKELRPQLEKL 482

BLAST of HG10015995 vs. TAIR 10
Match: AT4G30960.1 (SOS3-interacting protein 3 )

HSP 1 Score: 497.7 bits (1280), Expect = 1.0e-140
Identity = 248/436 (56.88%), Postives = 323/436 (74.08%), Query Frame = 0

Query: 21  LLLGRYEIGKLLGHGTFAKVYHARNIKTNESVAIKVIDKEKILKGGLIAHIKREISILRR 80
           LL GRYE+G+LLGHGTFAKVYHARNI+T +SVA+KV+ KEK++K G++  IKREIS++R 
Sbjct: 19  LLHGRYELGRLLGHGTFAKVYHARNIQTGKSVAMKVVGKEKVVKVGMVDQIKREISVMRM 78

Query: 81  VRHPNIVQLFEVMATKAKIYFVMEYVRGGELFKKVSKGRLKEEVARKYFQQLISAVAFCH 140
           V+HPNIV+L EVMA+K+KIYF ME VRGGELF KV+KGRL+E+VAR YFQQLISAV FCH
Sbjct: 79  VKHPNIVELHEVMASKSKIYFAMELVRGGELFAKVAKGRLREDVARVYFQQLISAVDFCH 138

Query: 141 ARGVYHRDLKPENLLLDENGNLKVSDFGLSAVSDQIRQDGLFHTFCGTPAYVAPEVLARK 200
           +RGVYHRDLKPENLLLDE GNLKV+DFGLSA ++ ++QDGL HT CGTPAYVAPEV+ +K
Sbjct: 139 SRGVYHRDLKPENLLLDEEGNLKVTDFGLSAFTEHLKQDGLLHTTCGTPAYVAPEVILKK 198

Query: 201 GYEAAKVDIWSCGVILFVLMAGYLPFHDQNIMAMYKKIYKGEFRCPRWFSPELIRLLTRL 260
           GY+ AK D+WSCGVILFVL+AGYLPF D N++ MY+KIY+G+F+CP W S +  RL+T+L
Sbjct: 199 GYDGAKADLWSCGVILFVLLAGYLPFQDDNLVNMYRKIYRGDFKCPGWLSSDARRLVTKL 258

Query: 261 LDTNPETRFTIPEIMENRWFKKGYKHIKFYIEDDKVCSVEDDNDDVDSLSDQSQSESDSE 320
           LD NP TR TI ++M++ WFKK     +    +    ++    +DVD L  +S+ E++  
Sbjct: 259 LDPNPNTRITIEKVMDSPWFKKQATRSR---NEPVAATITTTEEDVDFLVHKSKEETE-- 318

Query: 321 IIETRRKVTSLPRPASLNAFDIISFSPGFDLSGLFEDGGE----EARFVSNAPVSKIISK 380
                          +LNAF II+ S GFDLS LFE+  +    E RF ++ P S +IS 
Sbjct: 319 ---------------TLNAFHIIALSEGFDLSPLFEEKKKEEKREMRFATSRPASSVISS 378

Query: 381 LEEIAKLVS-FTVRKKDCRVSLEGSREGVKGPLTIAAEVFELTPNLVMVEVKRKGGDKAE 440
           LEE A++ + F VRK + RV +EG + G KG L + AE+F + P+ V+VEVK+  GD  E
Sbjct: 379 LEEAARVGNKFDVRKSESRVRIEGKQNGRKGKLAVEAEIFAVAPSFVVVEVKKDHGDTLE 434

Query: 441 YEQFCNNELKPALLNL 452
           Y  FC+  L+PAL ++
Sbjct: 439 YNNFCSTALRPALKDI 434

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_008439731.11.0e-26298.93PREDICTED: CBL-interacting serine/threonine-protein kinase 12-like [Cucumis melo... [more]
XP_038882513.11.7e-26298.71CBL-interacting serine/threonine-protein kinase 12-like [Benincasa hispida][more]
XP_011658253.15.5e-26198.07CBL-interacting serine/threonine-protein kinase 12 [Cucumis sativus] >KGN49312.1... [more]
XP_022926641.15.7e-25094.50CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita moschata] >KA... [more]
XP_023518648.17.5e-25094.29CBL-interacting serine/threonine-protein kinase 12-like [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
Q9SN433.2e-21982.25CBL-interacting serine/threonine-protein kinase 12 OS=Arabidopsis thaliana OX=37... [more]
Q9FJ555.1e-20175.81CBL-interacting serine/threonine-protein kinase 19 OS=Arabidopsis thaliana OX=37... [more]
Q9LP511.4e-19375.80CBL-interacting serine/threonine-protein kinase 18 OS=Arabidopsis thaliana OX=37... [more]
O229711.1e-17971.04CBL-interacting serine/threonine-protein kinase 13 OS=Arabidopsis thaliana OX=37... [more]
Q68Y495.5e-17965.62CBL-interacting protein kinase 19 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Match NameE-valueIdentityDescription
A0A5A7UB694.9e-26398.93Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AZF14.9e-26398.93Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KLP82.7e-26198.07Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6... [more]
A0A6J1EFG72.8e-25094.50Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1KSW81.8e-24994.08Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT4G18700.12.3e-22082.25CBL-interacting protein kinase 12 [more]
AT5G45810.13.6e-20275.81CBL-interacting protein kinase 19 [more]
AT1G29230.19.6e-19575.80CBL-interacting protein kinase 18 [more]
AT2G34180.17.9e-18171.04CBL-interacting protein kinase 13 [more]
AT4G30960.11.0e-14056.88SOS3-interacting protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Bottle gourd (Hangzhou Gourd) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 26..280
e-value: 8.6E-110
score: 380.7
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 26..280
e-value: 4.5E-76
score: 255.7
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 26..280
score: 52.484501
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 334..451
e-value: 2.9E-40
score: 139.2
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 17..333
e-value: 6.3E-107
score: 359.6
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 30..232
e-value: 2.0E-14
score: 51.4
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 26..239
e-value: 1.5E-36
score: 123.1
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 27..294
e-value: 1.1E-12
score: 45.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 302..325
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 19..461
NoneNo IPR availablePANTHERPTHR43895:SF96NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 19..461
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 25..279
e-value: 1.47425E-170
score: 477.667
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 338..448
e-value: 1.93649E-50
score: 165.438
IPR004041NAF domainPFAMPF03822NAFcoord: 334..390
e-value: 2.8E-24
score: 85.0
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 144..156
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 32..55
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 332..356
score: 14.624336
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 22..323

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
HG10015995.1HG10015995.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity