HG10015727 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10015727 vs. NCBI nr
Match: XP_038893257.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like [Benincasa hispida])

HSP 1 Score: 1429.8 bits (3700), Expect = 0.0e+00
Identity = 698/757 (92.21%), Postives = 707/757 (93.39%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLP+GNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPRGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSETEMSSDPQEIFAEYLNDFVL  GERILIYPSQKMI+WSGL+L+S QKQT+SKKG+D
Sbjct: 241 DSSETEMSSDPQEIFAEYLNDFVLCAGERILIYPSQKMITWSGLDLDSSQKQTVSKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGT  LQDLDSKGKRQPNLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTHLLQDLDSKGKRQPNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEV---------------------------------------KVRFVIMG 540
           DRYMIKTM+KAEV                                       KVRFVIMG
Sbjct: 481 DRYMIKTMKKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVRFVIMG 540

Query: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600
           NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV
Sbjct: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600

Query: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDPDYETDGQLRADKEQDS 660
           DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRD DYE DGQLRADK QDS
Sbjct: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDTDYEADGQLRADKAQDS 660

Query: 661 ARWASISLGINMPARAERTVRGGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 719
           ARWASISLGINMPARAERTVR GEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE
Sbjct: 661 ARWASISLGINMPARAERTVRRGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 720

BLAST of HG10015727 vs. NCBI nr
Match: XP_004142482.1 (phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis sativus] >KGN52322.1 hypothetical protein Csa_008424 [Cucumis sativus])

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 697/757 (92.07%), Postives = 707/757 (93.39%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQHSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSETEMSSDPQEIFAEYLNDFVL +GERILIYPSQKMISWSGL+L+SPQKQTISKKG+D
Sbjct: 241 DSSETEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNG GT QLQDLDSKGKRQ NLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGIGTHQLQDLDSKGKRQSNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QG+TISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGITISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEV---------------------------------------KVRFVIMG 540
           DRYMIKTMRKAEV                                       KVRFVIMG
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVRFVIMG 540

Query: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600
           NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV
Sbjct: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600

Query: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDPDYETDGQLRADKEQDS 660
           DKDCDFLEQERIMDYSLLVGLHFQETSYRE STP SHHSGS +PDYE DGQLRADKEQDS
Sbjct: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREVSTPGSHHSGS-NPDYEADGQLRADKEQDS 660

Query: 661 ARWASISLGINMPARAERTVRGGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 719
           ARWASISLGIN+PARAERTVR GEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE
Sbjct: 661 ARWASISLGINLPARAERTVRQGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 720

BLAST of HG10015727 vs. NCBI nr
Match: TYK09316.1 (phosphatidylinositol 4-phosphate 5-kinase 6 isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 695/781 (88.99%), Postives = 706/781 (90.40%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSE EMSSDPQEIFAEYLNDFVL +GERILIYPSQKMISWSGL+L+SPQKQTISKKG+D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNG GT  LQDLDSKGKRQ NLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGIGTHHLQDLDSKGKRQSNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEVK---------------------------------------------- 540
           DRYMIKTMRKAEVK                                              
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKDTKA 540

Query: 541 -----------------VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600
                            VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT
Sbjct: 541 VQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600

Query: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660
           LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES
Sbjct: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660

Query: 661 HHSGSRDPDYETDGQLRADKEQDSARWASISLGINMPARAERTVRGGEPEAQLVGEPTGE 719
           HHSGS +PDYE DGQLRA+KEQDSARWASISLGIN+PARAERTVR  EPEAQLVGEPTGE
Sbjct: 661 HHSGS-NPDYEADGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQLVGEPTGE 720

BLAST of HG10015727 vs. NCBI nr
Match: KAA0034762.1 (phosphatidylinositol 4-phosphate 5-kinase 6 [Cucumis melo var. makuwa])

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 689/781 (88.22%), Postives = 703/781 (90.01%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSE EMSSDPQEIFAEYLNDFVL +GERILIYPSQKMISWSGL+L+SPQKQTISKKG+D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNG GT  LQDLDSKGKRQ NLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGIGTHHLQDLDSKGKRQSNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEVK---------------------------------------------- 540
           DRYMIKTMRKAEVK                                              
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKDTKA 540

Query: 541 -----------------VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600
                            VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT
Sbjct: 541 VQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600

Query: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660
           LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES
Sbjct: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660

Query: 661 HHSGSRDPDYETDGQLRADKEQDSARWASISLGINMPARAERTVRGGEPEAQLVGEPTGE 719
           HHSG     ++++GQLRA+KEQDSARWASISLGIN+PARAERTVR  EPEAQLVGEPTGE
Sbjct: 661 HHSGFCCL-FDSNGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQLVGEPTGE 720

BLAST of HG10015727 vs. NCBI nr
Match: XP_023527539.1 (phosphatidylinositol 4-phosphate 5-kinase 6-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1333.5 bits (3450), Expect = 0.0e+00
Identity = 651/757 (86.00%), Postives = 681/757 (89.96%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYK+YAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHH EKAIPNGDF
Sbjct: 1   MYKDYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHTEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+ PHGHGKYLWTDGCMYVGEWYKGKT+GKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDSTPHGHGKYLWTDGCMYVGEWYKGKTIGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNL+HGQGTQNYANGDYYEGEWRRG QDGQGRYQWKNENHY+GQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLRHGQGTQNYANGDYYEGEWRRGCQDGQGRYQWKNENHYVGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           +NGK+SGNG+M+W +GNRYDGCWE+G PKG+GTFRWADGS YVGVWSKD TE TG FYPS
Sbjct: 181 RNGKMSGNGSMVWNDGNRYDGCWEEGFPKGDGTFRWADGSSYVGVWSKDSTELTGNFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           D+S  EMSS+P+E+  EYLND V+ +GERILIYPSQKMISWSGL+L+S  +QT+S+KGSD
Sbjct: 241 DTSGAEMSSNPRELLGEYLNDCVICSGERILIYPSQKMISWSGLDLDSLPRQTVSRKGSD 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           G RRLRRTSVDGK+SN+SFES ND SNE SSRNGKGT +LQDLDSKGKRQP   FQ A R
Sbjct: 301 GIRRLRRTSVDGKISNASFESFNDSSNEPSSRNGKGT-RLQDLDSKGKRQPTCGFQSANR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLF+VDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFEVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEV---------------------------------------KVRFVIMG 540
           DRYMIKTMRKAEV                                       KVRFVIMG
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRVTGTAQKKVRFVIMG 540

Query: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600
           NLFCSEYTIHRRFDLKGSS GRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV
Sbjct: 541 NLFCSEYTIHRRFDLKGSSLGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600

Query: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDPDYETDGQLRADKEQDS 660
           DKDCDFLEQERIMDYSLLVGLHFQETSY+EASTPESHHSGSRD D ETD   RADKEQDS
Sbjct: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYQEASTPESHHSGSRDSDCETDCLSRADKEQDS 660

Query: 661 ARWASISLGINMPARAERTVRGGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 719
           ARWASISLGINMPAR ERTVR GEPE QLVGEPTGEL+DVIIFFGIIDILQDYDISKKLE
Sbjct: 661 ARWASISLGINMPARVERTVRHGEPETQLVGEPTGELHDVIIFFGIIDILQDYDISKKLE 720

BLAST of HG10015727 vs. ExPASy Swiss-Prot
Match: Q9SFB8 (Phosphatidylinositol 4-phosphate 5-kinase 6 OS=Arabidopsis thaliana OX=3702 GN=PIP5K6 PE=2 SV=1)

HSP 1 Score: 983.4 bits (2541), Expect = 1.4e-285
Identity = 495/732 (67.62%), Postives = 567/732 (77.46%), Query Frame = 0

Query: 34  MSVAPADDEPG----PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWY 93
           MSVA ADD        GE +HAEKA+P+GDFYTGQW DN+PHGHGKYLWTDGCMYVG+W+
Sbjct: 1   MSVAHADDADDYSRPTGESYHAEKALPSGDFYTGQWRDNLPHGHGKYLWTDGCMYVGDWH 60

Query: 94  KGKTLGKGKFSWPSGATYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYAN 153
           +GKT+GKG+FSWPSGATYEGDFK+GYMDGKGTY  SSGD YRGSWVMNL+HGQGT++Y N
Sbjct: 61  RGKTMGKGRFSWPSGATYEGDFKNGYMDGKGTYIDSSGDLYRGSWVMNLRHGQGTKSYVN 120

Query: 154 GDYYEGEWRRGFQDGQGRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPK 213
           GD Y+GEWRRG QDG GRYQWKNENHYIGQWKNG ++GNGTMIW NGNRYDG WEDG PK
Sbjct: 121 GDCYDGEWRRGLQDGHGRYQWKNENHYIGQWKNGLMNGNGTMIWSNGNRYDGSWEDGAPK 180

Query: 214 GNGTFRWADGSCYVGVWSKDPTEQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGER 273
           GNGTFRW+DGS YVGVWSKDP EQ GT+YPS SS       PQ++F   L++ V+ T +R
Sbjct: 181 GNGTFRWSDGSFYVGVWSKDPKEQNGTYYPSTSS-GNFDWQPQQVFYVDLSECVVCTCQR 240

Query: 274 ILIYPSQKMISWSGLELESPQKQTISKKGSDGNRRLRRTSVDGKVSNSSFESLNDGSNEA 333
           I + PSQKM  W G       +Q+ S   +  + R RR SVDG+VSN   E  +      
Sbjct: 241 IPVLPSQKMPVWYG-----ASEQSSSGNRTKNSERPRRRSVDGRVSNGEMELRS------ 300

Query: 334 SSRNGKGTPQLQD-LDSKGKRQPNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGR 393
              NG G  Q+ D  +S       LR QPAK+QG TISKGHKNYELMLNLQLGIRHSVGR
Sbjct: 301 ---NGSGYLQVDDNAESTRSSLGPLRIQPAKKQGQTISKGHKNYELMLNLQLGIRHSVGR 360

Query: 394 PAPATSLDLKSSAFDPKEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVD 453
           PAPATSLDLK+SAFDPKEK+WTKFP EGSK+TPPHQS +F+WKDYCPVVFRTLRKLF VD
Sbjct: 361 PAPATSLDLKASAFDPKEKLWTKFPSEGSKYTPPHQSCEFKWKDYCPVVFRTLRKLFSVD 420

Query: 454 PADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEV--------------- 513
            ADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTM+KAE                
Sbjct: 421 AADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKAETKVLIRMLPAYYNHVR 480

Query: 514 ------------------------KVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPE 573
                                   KVRFVIMGNLFC+ ++IHRRFDLKGSSHGR+T KPE
Sbjct: 481 ACENTLVTKFFGLHCVKLTGTAQKKVRFVIMGNLFCTGHSIHRRFDLKGSSHGRLTTKPE 540

Query: 574 AEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSY 633
           +EID  TTLKDLDLN+ FRLQK WFQEFCRQVD+DC+FLEQERIMDYSLLVGLHF+E + 
Sbjct: 541 SEIDPNTTLKDLDLNFAFRLQKNWFQEFCRQVDRDCEFLEQERIMDYSLLVGLHFREAAI 600

Query: 634 REASTPESHHSGSRDPDYETDGQL-RADKEQ---DSARWASISLGINMPARAERTVRGGE 693
           ++++TP    SG+R P   ++ +L RA+ ++   D+++ ASI LGINMPAR ERT R  +
Sbjct: 601 KDSATPT---SGARTPTGNSETRLSRAEMDRFLLDASKLASIKLGINMPARVERTARRSD 660

Query: 694 PEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKR 718
            E QLVG+PTGE YDVI++FGIIDILQDYDISKKLEHAYKS QYDP+SISAVDPKQYS+R
Sbjct: 661 CENQLVGDPTGEFYDVIVYFGIIDILQDYDISKKLEHAYKSMQYDPTSISAVDPKQYSRR 714

BLAST of HG10015727 vs. ExPASy Swiss-Prot
Match: Q9M1K2 (Phosphatidylinositol 4-phosphate 5-kinase 4 OS=Arabidopsis thaliana OX=3702 GN=PIP5K4 PE=4 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 2.3e-269
Identity = 483/806 (59.93%), Postives = 575/806 (71.34%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGP--------------- 60
           M KE + ++KAWE TVRKTQ  AKKRANSIF  +SVAP  D+                  
Sbjct: 1   MSKEQSCVLKAWEVTVRKTQ-QAKKRANSIFGTVSVAPQTDDDATTTTEENDDETSTNRS 60

Query: 61  --GELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPS 120
             GEL+HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G+GKF WPS
Sbjct: 61  SIGELYHAERILPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGRGKFGWPS 120

Query: 121 GATYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQD 180
           GATYEG+FKSGYMDG G YTG SGDTY+G WVMNLKHG G + +ANGD Y+GEWRRG Q+
Sbjct: 121 GATYEGEFKSGYMDGVGLYTGPSGDTYKGQWVMNLKHGHGIKRFANGDVYDGEWRRGLQE 180

Query: 181 GQGRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYV 240
            QG+YQW++ ++Y+G+WKN  I G GT IW +GNRYDG W+DG P+GNGTF+WADGS YV
Sbjct: 181 AQGKYQWRDGSYYMGEWKNATICGKGTFIWTDGNRYDGFWDDGFPRGNGTFKWADGSFYV 240

Query: 241 GVWSKDPTEQTGTFYPS-DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMIS-W 300
           G WS DP E  GT+YPS D    E   DP+++F   L+++ + +GER+ + PSQK +S W
Sbjct: 241 GHWSNDPEEMNGTYYPSGDDGSPEW--DPKDVFTN-LSEYKICSGERVPVLPSQKKLSVW 300

Query: 301 -SGLELESPQKQTISKKGSDGNRRLRRTSVDGKVS---NSSFESLNDGSNEASSRNGKGT 360
            S   +E P                RR SVDG+VS   + +FE +N    E    +G+G 
Sbjct: 301 NSSKRVEKP----------------RRMSVDGRVSVGVDRAFEKMNMWGTE----SGEGA 360

Query: 361 PQL-----QDLDSKGKRQ------PNLRFQPA--------KRQGVTISKGHKNYELMLNL 420
             +     +DLD++  R        +LR  PA        ++QG TISKGH+NYELMLNL
Sbjct: 361 ADIDSTTRRDLDAEMMRLEAEGFIQSLRPSPAPMRLPRAGRKQGETISKGHRNYELMLNL 420

Query: 421 QLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVF 480
           QLGIRH+VG+ AP  SLDLK SAFDPKEKVWT+FPPEG+K+TPPHQSS+F+WKDYCP+VF
Sbjct: 421 QLGIRHAVGKQAPVVSLDLKHSAFDPKEKVWTRFPPEGTKYTPPHQSSEFKWKDYCPLVF 480

Query: 481 RTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEV----- 540
           R+LRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTM+K+E      
Sbjct: 481 RSLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLR 540

Query: 541 ----------------------------------KVRFVIMGNLFCSEYTIHRRFDLKGS 600
                                             KVRFVIMGNLFCSEY+IHRRFDLKGS
Sbjct: 541 MLAAYYNHVRAFENTLVIRFYGLHCVKLTGPIQKKVRFVIMGNLFCSEYSIHRRFDLKGS 600

Query: 601 SHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLL 660
           S GR TDKPE+EI+  T LKDLDLN+IFRLQK W+QEF RQVDKDC+FLEQERIMDYSLL
Sbjct: 601 SLGRTTDKPESEINSNTILKDLDLNFIFRLQKAWYQEFIRQVDKDCEFLEQERIMDYSLL 660

Query: 661 VGLHFQETSYREASTPESHHSGSRDPDYETDGQ-----LRADKEQ---DSARWASISLGI 718
           VG+HF+E S      P    SG+R P  E + +      RAD +Q   D  RWASI LG 
Sbjct: 661 VGIHFREASVAGELIP----SGARTPIGEFEDESAPRLSRADVDQLLSDPTRWASIRLGG 720

BLAST of HG10015727 vs. ExPASy Swiss-Prot
Match: Q9SLG9 (Phosphatidylinositol 4-phosphate 5-kinase 5 OS=Arabidopsis thaliana OX=3702 GN=PIP5K5 PE=2 SV=1)

HSP 1 Score: 926.8 bits (2394), Expect = 1.5e-268
Identity = 472/794 (59.45%), Postives = 574/794 (72.29%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAP----------ADDEPGPG---- 60
           M K+ + ++KAWE TVRKTQ  AKKRANS+F  +SVAP           DDE        
Sbjct: 1   MSKDQSYVLKAWEVTVRKTQ-QAKKRANSVFGTVSVAPHTDNDTTTDDNDDETTTNRSSL 60

Query: 61  -ELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGA 120
            E +HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G GKF WPSGA
Sbjct: 61  EEFYHAERVLPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGA 120

Query: 121 TYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQ 180
           TYEG+FKSGYMDG GTYTG SGD Y+G WVMNLKHG G +++ANGD Y+GEWRRG Q+GQ
Sbjct: 121 TYEGEFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQ 180

Query: 181 GRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGV 240
           G+YQW + ++YIG+WKNG I G G+ +W NGNRYDG W++G P+GNGTF+W +GS YVG 
Sbjct: 181 GKYQWSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGH 240

Query: 241 WSKDPTEQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMIS-W-SG 300
           WSKDP E  GT+YPS  +E  +  DP+++F   L+++ + +GER+   PSQK +S W S 
Sbjct: 241 WSKDPEEMNGTYYPS-GNEGNLEWDPKDLF-NNLSEYTICSGERVPTLPSQKKLSVWNSS 300

Query: 301 LELESPQKQTISKKGSDGNRRLRRTSVDGKVS---NSSFESLNDGSNE----ASSRNGKG 360
             +E P                RRTSVDG+VS   + +FE +N   NE     +    + 
Sbjct: 301 KRIEKP----------------RRTSVDGRVSVGVDRAFEKMNMWGNEIGEGGADMRKEL 360

Query: 361 TPQLQDLDSKGKRQ------PNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPA 420
             +L  LD++G +       P    +  ++QG TISKGH+NYELMLNLQLGIRHSVGR A
Sbjct: 361 DAELMRLDAEGLQSLKSSPVPMKLPKAGRKQGETISKGHRNYELMLNLQLGIRHSVGRQA 420

Query: 421 PATSLDLKSSAFDPKEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPA 480
           PA SLDLK SAFDPK+K+W +FP EG+K+TPPHQS++F+WKDYCP+VFR+LRKLFKVDPA
Sbjct: 421 PAASLDLKPSAFDPKDKIWRRFPREGTKYTPPHQSTEFKWKDYCPLVFRSLRKLFKVDPA 480

Query: 481 DYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEV----------------- 540
           DYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTM+K+E                  
Sbjct: 481 DYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLGMLAAYYNHVRAF 540

Query: 541 ----------------------KVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAE 600
                                 KVRFVIMGNLFCS+Y++HRRFDLKGSS GR TDKPE+E
Sbjct: 541 ENSLVIRFFGLHCVKLNGPTQKKVRFVIMGNLFCSKYSVHRRFDLKGSSLGRTTDKPESE 600

Query: 601 IDGTTTLKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYRE 660
           ID  T LKDLDLN+IFRLQK W+QEF RQ+DKDC+FLEQERIMDYSLLVG+HF+E S   
Sbjct: 601 IDSNTILKDLDLNFIFRLQKAWYQEFIRQIDKDCEFLEQERIMDYSLLVGIHFREASVAG 660

Query: 661 ASTPESHHSGSRDPDYETDGQ-----LRADKEQ---DSARWASISLGINMPARAERTVRG 718
              P    SG+R P  E++ +      RA+ ++   D +RWASI LG NMPARAERT+R 
Sbjct: 661 ELIP----SGARTPIGESEEESGPRLSRAEVDELLSDPSRWASIRLGTNMPARAERTMRK 720

BLAST of HG10015727 vs. ExPASy Swiss-Prot
Match: Q56YP2 (Phosphatidylinositol 4-phosphate 5-kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PIP5K1 PE=1 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 2.8e-198
Identity = 373/726 (51.38%), Postives = 464/726 (63.91%), Query Frame = 0

Query: 45  PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSG 104
           P  L   EK +PNGD Y G +    PHG GKYLW DGCMY G+W +GK  GKGKFSWPSG
Sbjct: 65  PTPLVDVEKPLPNGDLYIGSFSGGFPHGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSG 124

Query: 105 ATYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDG 164
           ATYEG+FKSG M+G GT+TG+ GDTYRG+WV + KHG G + YANGD+YEG WRR  QDG
Sbjct: 125 ATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDG 184

Query: 165 QGRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVG 224
           +GRY W+N N Y G+W++G ISG G ++W NGNRY+G WE+G+PKGNG F W+DGS  VG
Sbjct: 185 RGRYVWRNGNQYTGEWRSGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVG 244

Query: 225 VWSKDPTEQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGL 284
            W++                           +  +  F     +  LI  ++K       
Sbjct: 245 AWNE---------------------------SNIMRSFFNGVEKNDLIVGNRKR------ 304

Query: 285 ELESPQKQTISKKGSDGNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLD 344
              S      S  G     R+     DG+  + + + ++  + EAS          +D  
Sbjct: 305 --SSVDSGAGSLGGEKVFPRICIWESDGEAGDITCDIID--NVEASMIYRDRISVDRDGF 364

Query: 345 SKGKRQPNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDP 404
            + K+ P      AK+ G TISKGHK Y+LMLNLQLGIR+SVG+ A     DLK + FDP
Sbjct: 365 RQFKKNPCWFNGEAKKPGQTISKGHKKYDLMLNLQLGIRYSVGKHASIVR-DLKQTDFDP 424

Query: 405 KEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRE 464
           KEK WT+FPPEG+K TPPHQS DFRWKDYCP+VFR LR+LF+VDPA YML+ICGNDALRE
Sbjct: 425 KEKFWTRFPPEGTKTTPPHQSVDFRWKDYCPLVFRRLRELFQVDPAKYMLAICGNDALRE 484

Query: 465 LSSPGKSGSFFYLTHDDRYMIKTMRKAEVKV----------------------------- 524
           LSSPGKSGSFFYLT DDR+MIKT++K+EVKV                             
Sbjct: 485 LSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENSLVTRFYGVHCV 544

Query: 525 --------RFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIF 584
                   RF++MGNLFCSEY I RRFDLKGSSHGR T KPE EID TTTLKDLDLN+ F
Sbjct: 545 KPVGGQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRSTAKPEGEIDETTTLKDLDLNFSF 604

Query: 585 RLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQE--TSYREASTPESHHSGSRDP 644
           RLQ+ W+QE  +Q+ +DC+FLE ERIMDYSLLVG+HF++  T  +   +P    SG  D 
Sbjct: 605 RLQRNWYQELMKQIKRDCEFLEAERIMDYSLLVGVHFRDDNTGEKMGLSPFVLRSGRID- 664

Query: 645 DYETDGQLRADK-------EQD---SARWASISLGINMPARAERTVRGGEPEAQLVGEPT 704
            Y+ +  +R  +       + D   + R  SI LG NMPA+AER  R  + +    G  +
Sbjct: 665 SYQNEKFMRGCRFLEAELQDMDRILAGRKPSIRLGANMPAKAERMARRSDFDQYSSGGAS 724

Query: 705 ----GELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIF 718
               GE+Y+V+++FG+IDILQDYDI+KK+EHAYKS Q DP+SISAVDPK YSKRFRDFI 
Sbjct: 725 YPSHGEMYEVVLYFGVIDILQDYDITKKIEHAYKSLQADPASISAVDPKLYSKRFRDFIS 751

BLAST of HG10015727 vs. ExPASy Swiss-Prot
Match: Q8L796 (Phosphatidylinositol 4-phosphate 5-kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PIP5K2 PE=1 SV=2)

HSP 1 Score: 684.9 bits (1766), Expect = 1.0e-195
Identity = 370/723 (51.18%), Postives = 454/723 (62.79%), Query Frame = 0

Query: 52  EKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDF 111
           EK +PNGD Y G +    P+G GKYLW DGCMY GEW +GK  GKGKFSWPSGATYEG+F
Sbjct: 73  EKPLPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGEF 132

Query: 112 KSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWK 171
           KSG M+G GT+ G  GDTYRGSWV + K G G + YANGDYYEG WRR  QDG+GRY W 
Sbjct: 133 KSGRMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVWM 192

Query: 172 NENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPT 231
           N N Y G+W+NG I G G + W NGNRY+G WE+G+PKG+G F WADGS ++G W++   
Sbjct: 193 NGNQYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSWNESSN 252

Query: 232 EQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQK 291
                F   + +E  +++  +             TGE+I      ++  W          
Sbjct: 253 LMRNFFDGIEKNELIVATRKRSSVDSGAGSL---TGEKIF----PRICIWES-------- 312

Query: 292 QTISKKGSDGNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQ- 351
                              DG+  + + + +++       R+         +D  G RQ 
Sbjct: 313 -------------------DGEAGDITCDIVDNVEASVIYRD------RISIDKDGFRQF 372

Query: 352 ---PNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEK 411
              P      AK+ G TISKGHK Y+LMLNLQ GIR+SVG+ A     DLK S FDP EK
Sbjct: 373 RKNPCCFSGEAKKPGETISKGHKKYDLMLNLQHGIRYSVGKHASVVR-DLKQSDFDPSEK 432

Query: 412 VWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSS 471
            WT+FPPEGSK TPPH S DFRWKDYCP+VFR LR+LF VDPADYML+ICGNDALRELSS
Sbjct: 433 FWTRFPPEGSKTTPPHLSVDFRWKDYCPLVFRRLRELFTVDPADYMLAICGNDALRELSS 492

Query: 472 PGKSGSFFYLTHDDRYMIKTMRKAEVKV-------------------------------- 531
           PGKSGSFFYLT DDR+MIKT++K+EVKV                                
Sbjct: 493 PGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENTLVTRFYGVHCIKPV 552

Query: 532 -----RFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQ 591
                RF++MGNLFCSEY I RRFDLKGSSHGR T KPE EID TTTLKDLDLN+ FRLQ
Sbjct: 553 GGQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRYTSKPEGEIDETTTLKDLDLNFAFRLQ 612

Query: 592 KIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQE--TSYREASTPESHHSGSRDPDYE 651
           + W+QE   Q+ +DC+FLE ERIMDYSLLVG+HF++  T  +   +P    SG  +  Y+
Sbjct: 613 RNWYQELMTQIKRDCEFLEAERIMDYSLLVGVHFRDDNTGDKMGLSPFVLRSGKIE-SYQ 672

Query: 652 TDGQLRADK-------EQD---SARWASISLGINMPARAERTVRGGEPEAQLVG----EP 711
           ++  +R  +       + D   + R   I LG NMPARAER  R  + +    G    + 
Sbjct: 673 SEKFMRGCRFLEAELQDMDRILAGRKPLIRLGANMPARAERMARRSDYDQYSSGGTNYQS 732

Query: 712 TGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIFRIF 718
            GE+Y+V+++FGIIDILQDYDISKK+EHAYKS Q DP+SISAVDPK YS+RFRDFI RIF
Sbjct: 733 HGEVYEVVLYFGIIDILQDYDISKKIEHAYKSLQADPASISAVDPKLYSRRFRDFISRIF 753

BLAST of HG10015727 vs. ExPASy TrEMBL
Match: A0A0A0KV49 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_5G623850 PE=4 SV=1)

HSP 1 Score: 1423.3 bits (3683), Expect = 0.0e+00
Identity = 697/757 (92.07%), Postives = 707/757 (93.39%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQ+SAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQHSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSETEMSSDPQEIFAEYLNDFVL +GERILIYPSQKMISWSGL+L+SPQKQTISKKG+D
Sbjct: 241 DSSETEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNG GT QLQDLDSKGKRQ NLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGIGTHQLQDLDSKGKRQSNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QG+TISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGITISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEV---------------------------------------KVRFVIMG 540
           DRYMIKTMRKAEV                                       KVRFVIMG
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVRFVIMG 540

Query: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600
           NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV
Sbjct: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600

Query: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDPDYETDGQLRADKEQDS 660
           DKDCDFLEQERIMDYSLLVGLHFQETSYRE STP SHHSGS +PDYE DGQLRADKEQDS
Sbjct: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREVSTPGSHHSGS-NPDYEADGQLRADKEQDS 660

Query: 661 ARWASISLGINMPARAERTVRGGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 719
           ARWASISLGIN+PARAERTVR GEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE
Sbjct: 661 ARWASISLGINLPARAERTVRQGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 720

BLAST of HG10015727 vs. ExPASy TrEMBL
Match: A0A5D3CG23 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003130 PE=4 SV=1)

HSP 1 Score: 1409.4 bits (3647), Expect = 0.0e+00
Identity = 695/781 (88.99%), Postives = 706/781 (90.40%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSE EMSSDPQEIFAEYLNDFVL +GERILIYPSQKMISWSGL+L+SPQKQTISKKG+D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNG GT  LQDLDSKGKRQ NLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGIGTHHLQDLDSKGKRQSNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEVK---------------------------------------------- 540
           DRYMIKTMRKAEVK                                              
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKDTKA 540

Query: 541 -----------------VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600
                            VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT
Sbjct: 541 VQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600

Query: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660
           LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES
Sbjct: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660

Query: 661 HHSGSRDPDYETDGQLRADKEQDSARWASISLGINMPARAERTVRGGEPEAQLVGEPTGE 719
           HHSGS +PDYE DGQLRA+KEQDSARWASISLGIN+PARAERTVR  EPEAQLVGEPTGE
Sbjct: 661 HHSGS-NPDYEADGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQLVGEPTGE 720

BLAST of HG10015727 vs. ExPASy TrEMBL
Match: A0A5A7SUC1 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007860 PE=4 SV=1)

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 689/781 (88.22%), Postives = 703/781 (90.01%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF
Sbjct: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+MPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDHMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNLKHGQGTQNYANGDYY+GEWRRGFQDGQGRYQWKNENHYIGQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLKHGQGTQNYANGDYYDGEWRRGFQDGQGRYQWKNENHYIGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           KNGKI+GNGTMIW NGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS
Sbjct: 181 KNGKINGNGTMIWNNGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           DSSE EMSSDPQEIFAEYLNDFVL +GERILIYPSQKMISWSGL+L+SPQKQTISKKG+D
Sbjct: 241 DSSEAEMSSDPQEIFAEYLNDFVLCSGERILIYPSQKMISWSGLDLDSPQKQTISKKGND 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNG GT  LQDLDSKGKRQ NLRFQPAKR
Sbjct: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGIGTHHLQDLDSKGKRQSNLRFQPAKR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSK+T
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKYT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEVK---------------------------------------------- 540
           DRYMIKTMRKAEVK                                              
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRLTGTSQKKVYKDTKA 540

Query: 541 -----------------VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600
                            VRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT
Sbjct: 541 VQWLPWILQFTDFVDLQVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTT 600

Query: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660
           LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES
Sbjct: 601 LKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYREASTPES 660

Query: 661 HHSGSRDPDYETDGQLRADKEQDSARWASISLGINMPARAERTVRGGEPEAQLVGEPTGE 719
           HHSG     ++++GQLRA+KEQDSARWASISLGIN+PARAERTVR  EPEAQLVGEPTGE
Sbjct: 661 HHSGFCCL-FDSNGQLRANKEQDSARWASISLGINLPARAERTVRRAEPEAQLVGEPTGE 720

BLAST of HG10015727 vs. ExPASy TrEMBL
Match: A0A6J1JKV8 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111486113 PE=4 SV=1)

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 649/757 (85.73%), Postives = 681/757 (89.96%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYK+YAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHH EKAIPNGDF
Sbjct: 1   MYKDYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHTEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+ PHGHGKYLWTDGCMYVGEWYKGKT+GKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDSTPHGHGKYLWTDGCMYVGEWYKGKTIGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNL+HGQGTQNYANGDYYEGEWRRG QDGQGRYQWKNENHY+GQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLRHGQGTQNYANGDYYEGEWRRGCQDGQGRYQWKNENHYVGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           +NGK+SGNG+M+W +GNRYDGCWE+G PKG+GTFRWADGS YVGVWSKD TE TG+FYPS
Sbjct: 181 RNGKMSGNGSMVWNDGNRYDGCWEEGFPKGDGTFRWADGSSYVGVWSKDSTELTGSFYPS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           D+S TEMSS+P E+  EYLND V+ +GERILIYPSQKMISWSGL+L+S  +QT+S+KGSD
Sbjct: 241 DTSGTEMSSNPHELLGEYLNDCVICSGERILIYPSQKMISWSGLDLDSLPRQTVSRKGSD 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           G RRLRRTSVDGK+SN+ FES NDG NE SSRNGKGT +L DLDSKGKRQPN  FQ A +
Sbjct: 301 GIRRLRRTSVDGKISNARFESFNDGLNEPSSRNGKGT-RLLDLDSKGKRQPNSGFQSANK 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLF+VDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFEVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEV---------------------------------------KVRFVIMG 540
           DRYMIKTM+KAEV                                       KVRFVIMG
Sbjct: 481 DRYMIKTMKKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRVTGTAQKKVRFVIMG 540

Query: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600
           NLFCSEYTIHRRFDLKGSS GRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV
Sbjct: 541 NLFCSEYTIHRRFDLKGSSLGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600

Query: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDPDYETDGQLRADKEQDS 660
           DKDCDFLEQERIMDYSLLVGLHFQETSY+EASTPESHHSGSRD D ETD   RADKEQDS
Sbjct: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYQEASTPESHHSGSRDSDCETDCLSRADKEQDS 660

Query: 661 ARWASISLGINMPARAERTVRGGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 719
           ARWASISLGINMPAR ERTVR GEPE QLVGEPTGEL+DVIIFFGIIDILQDYDISKKLE
Sbjct: 661 ARWASISLGINMPARVERTVRHGEPETQLVGEPTGELHDVIIFFGIIDILQDYDISKKLE 720

BLAST of HG10015727 vs. ExPASy TrEMBL
Match: A0A6J1GZA6 (Phosphatidylinositol 4-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111458505 PE=4 SV=1)

HSP 1 Score: 1329.7 bits (3440), Expect = 0.0e+00
Identity = 650/757 (85.87%), Postives = 680/757 (89.83%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHAEKAIPNGDF 60
           MYK+YAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHH EKAIPNGDF
Sbjct: 1   MYKDYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGPGELHHTEKAIPNGDF 60

Query: 61  YTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDFKSGYMDGKG 120
           YTGQWMD+ PHGHGKYLWTDGCMYVGEWYKGKT+GKGKFSWPSGATYEGDFKSGYMDGKG
Sbjct: 61  YTGQWMDSTPHGHGKYLWTDGCMYVGEWYKGKTIGKGKFSWPSGATYEGDFKSGYMDGKG 120

Query: 121 TYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWKNENHYIGQW 180
           TYTGSSGDTYRG WVMNL+HGQGTQNYANGDYYEGEWRRG QDGQGRYQWKNENHY+GQW
Sbjct: 121 TYTGSSGDTYRGCWVMNLRHGQGTQNYANGDYYEGEWRRGCQDGQGRYQWKNENHYVGQW 180

Query: 181 KNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPTEQTGTFYPS 240
           +NGK+SGNG+M+W +GNRYDGCWE+G PKG+GTFRWADGS YVGVWSKD TE TG FY S
Sbjct: 181 RNGKMSGNGSMVWNDGNRYDGCWEEGFPKGDGTFRWADGSSYVGVWSKDSTELTGNFYTS 240

Query: 241 DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQKQTISKKGSD 300
           D+S TEMSS+P+E+  EYLND V+ +GERILIYPSQKMISWSGL+L+S  +QT+S+KGSD
Sbjct: 241 DTSGTEMSSNPRELLGEYLNDCVICSGERILIYPSQKMISWSGLDLDSLPRQTVSRKGSD 300

Query: 301 GNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQPNLRFQPAKR 360
           G RRLRRTSVDGK+SN+SFES ND SNE SSRNGKGT +L DLDSKGKRQP   FQ A R
Sbjct: 301 GIRRLRRTSVDGKISNASFESFNDSSNEPSSRNGKGT-RLLDLDSKGKRQPTCGFQSANR 360

Query: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420
           QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT
Sbjct: 361 QGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHT 420

Query: 421 PPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480
           PPHQSSDFRWKDYCPVVFRTLRKLF+VDPADYMLSICGNDALRELSSPGKSGSFFYLTHD
Sbjct: 421 PPHQSSDFRWKDYCPVVFRTLRKLFEVDPADYMLSICGNDALRELSSPGKSGSFFYLTHD 480

Query: 481 DRYMIKTMRKAEV---------------------------------------KVRFVIMG 540
           DRYMIKTMRKAEV                                       KVRFVIMG
Sbjct: 481 DRYMIKTMRKAEVKVLLRMLPAYYKHVRSFENTLVTKFYGLHCVRVTGTAQKKVRFVIMG 540

Query: 541 NLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600
           NLFCSEYTIHRRFDLKGSS GRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV
Sbjct: 541 NLFCSEYTIHRRFDLKGSSLGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQV 600

Query: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYREASTPESHHSGSRDPDYETDGQLRADKEQDS 660
           DKDCDFLEQERIMDYSLLVGLHFQETSY+EASTPESHHSGSRD D ETD   RADKEQDS
Sbjct: 601 DKDCDFLEQERIMDYSLLVGLHFQETSYQEASTPESHHSGSRDSDCETDCLSRADKEQDS 660

Query: 661 ARWASISLGINMPARAERTVRGGEPEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLE 719
           ARWASISLGINMPAR ERTVR GEPE QLVGEPTGEL+DVIIFFGIIDILQDYDISKKLE
Sbjct: 661 ARWASISLGINMPARVERTVRHGEPETQLVGEPTGELHDVIIFFGIIDILQDYDISKKLE 720

BLAST of HG10015727 vs. TAIR 10
Match: AT3G07960.1 (Phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 983.4 bits (2541), Expect = 9.6e-287
Identity = 495/732 (67.62%), Postives = 567/732 (77.46%), Query Frame = 0

Query: 34  MSVAPADDEPG----PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWY 93
           MSVA ADD        GE +HAEKA+P+GDFYTGQW DN+PHGHGKYLWTDGCMYVG+W+
Sbjct: 1   MSVAHADDADDYSRPTGESYHAEKALPSGDFYTGQWRDNLPHGHGKYLWTDGCMYVGDWH 60

Query: 94  KGKTLGKGKFSWPSGATYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYAN 153
           +GKT+GKG+FSWPSGATYEGDFK+GYMDGKGTY  SSGD YRGSWVMNL+HGQGT++Y N
Sbjct: 61  RGKTMGKGRFSWPSGATYEGDFKNGYMDGKGTYIDSSGDLYRGSWVMNLRHGQGTKSYVN 120

Query: 154 GDYYEGEWRRGFQDGQGRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPK 213
           GD Y+GEWRRG QDG GRYQWKNENHYIGQWKNG ++GNGTMIW NGNRYDG WEDG PK
Sbjct: 121 GDCYDGEWRRGLQDGHGRYQWKNENHYIGQWKNGLMNGNGTMIWSNGNRYDGSWEDGAPK 180

Query: 214 GNGTFRWADGSCYVGVWSKDPTEQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGER 273
           GNGTFRW+DGS YVGVWSKDP EQ GT+YPS SS       PQ++F   L++ V+ T +R
Sbjct: 181 GNGTFRWSDGSFYVGVWSKDPKEQNGTYYPSTSS-GNFDWQPQQVFYVDLSECVVCTCQR 240

Query: 274 ILIYPSQKMISWSGLELESPQKQTISKKGSDGNRRLRRTSVDGKVSNSSFESLNDGSNEA 333
           I + PSQKM  W G       +Q+ S   +  + R RR SVDG+VSN   E  +      
Sbjct: 241 IPVLPSQKMPVWYG-----ASEQSSSGNRTKNSERPRRRSVDGRVSNGEMELRS------ 300

Query: 334 SSRNGKGTPQLQD-LDSKGKRQPNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGR 393
              NG G  Q+ D  +S       LR QPAK+QG TISKGHKNYELMLNLQLGIRHSVGR
Sbjct: 301 ---NGSGYLQVDDNAESTRSSLGPLRIQPAKKQGQTISKGHKNYELMLNLQLGIRHSVGR 360

Query: 394 PAPATSLDLKSSAFDPKEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVD 453
           PAPATSLDLK+SAFDPKEK+WTKFP EGSK+TPPHQS +F+WKDYCPVVFRTLRKLF VD
Sbjct: 361 PAPATSLDLKASAFDPKEKLWTKFPSEGSKYTPPHQSCEFKWKDYCPVVFRTLRKLFSVD 420

Query: 454 PADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEV--------------- 513
            ADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTM+KAE                
Sbjct: 421 AADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKAETKVLIRMLPAYYNHVR 480

Query: 514 ------------------------KVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPE 573
                                   KVRFVIMGNLFC+ ++IHRRFDLKGSSHGR+T KPE
Sbjct: 481 ACENTLVTKFFGLHCVKLTGTAQKKVRFVIMGNLFCTGHSIHRRFDLKGSSHGRLTTKPE 540

Query: 574 AEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSY 633
           +EID  TTLKDLDLN+ FRLQK WFQEFCRQVD+DC+FLEQERIMDYSLLVGLHF+E + 
Sbjct: 541 SEIDPNTTLKDLDLNFAFRLQKNWFQEFCRQVDRDCEFLEQERIMDYSLLVGLHFREAAI 600

Query: 634 REASTPESHHSGSRDPDYETDGQL-RADKEQ---DSARWASISLGINMPARAERTVRGGE 693
           ++++TP    SG+R P   ++ +L RA+ ++   D+++ ASI LGINMPAR ERT R  +
Sbjct: 601 KDSATPT---SGARTPTGNSETRLSRAEMDRFLLDASKLASIKLGINMPARVERTARRSD 660

Query: 694 PEAQLVGEPTGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKR 718
            E QLVG+PTGE YDVI++FGIIDILQDYDISKKLEHAYKS QYDP+SISAVDPKQYS+R
Sbjct: 661 CENQLVGDPTGEFYDVIVYFGIIDILQDYDISKKLEHAYKSMQYDPTSISAVDPKQYSRR 714

BLAST of HG10015727 vs. TAIR 10
Match: AT3G56960.1 (phosphatidyl inositol monophosphate 5 kinase 4 )

HSP 1 Score: 929.5 bits (2401), Expect = 1.7e-270
Identity = 483/806 (59.93%), Postives = 575/806 (71.34%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAPADDEPGP--------------- 60
           M KE + ++KAWE TVRKTQ  AKKRANSIF  +SVAP  D+                  
Sbjct: 1   MSKEQSCVLKAWEVTVRKTQ-QAKKRANSIFGTVSVAPQTDDDATTTTEENDDETSTNRS 60

Query: 61  --GELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPS 120
             GEL+HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G+GKF WPS
Sbjct: 61  SIGELYHAERILPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGRGKFGWPS 120

Query: 121 GATYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQD 180
           GATYEG+FKSGYMDG G YTG SGDTY+G WVMNLKHG G + +ANGD Y+GEWRRG Q+
Sbjct: 121 GATYEGEFKSGYMDGVGLYTGPSGDTYKGQWVMNLKHGHGIKRFANGDVYDGEWRRGLQE 180

Query: 181 GQGRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYV 240
            QG+YQW++ ++Y+G+WKN  I G GT IW +GNRYDG W+DG P+GNGTF+WADGS YV
Sbjct: 181 AQGKYQWRDGSYYMGEWKNATICGKGTFIWTDGNRYDGFWDDGFPRGNGTFKWADGSFYV 240

Query: 241 GVWSKDPTEQTGTFYPS-DSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMIS-W 300
           G WS DP E  GT+YPS D    E   DP+++F   L+++ + +GER+ + PSQK +S W
Sbjct: 241 GHWSNDPEEMNGTYYPSGDDGSPEW--DPKDVFTN-LSEYKICSGERVPVLPSQKKLSVW 300

Query: 301 -SGLELESPQKQTISKKGSDGNRRLRRTSVDGKVS---NSSFESLNDGSNEASSRNGKGT 360
            S   +E P                RR SVDG+VS   + +FE +N    E    +G+G 
Sbjct: 301 NSSKRVEKP----------------RRMSVDGRVSVGVDRAFEKMNMWGTE----SGEGA 360

Query: 361 PQL-----QDLDSKGKRQ------PNLRFQPA--------KRQGVTISKGHKNYELMLNL 420
             +     +DLD++  R        +LR  PA        ++QG TISKGH+NYELMLNL
Sbjct: 361 ADIDSTTRRDLDAEMMRLEAEGFIQSLRPSPAPMRLPRAGRKQGETISKGHRNYELMLNL 420

Query: 421 QLGIRHSVGRPAPATSLDLKSSAFDPKEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVF 480
           QLGIRH+VG+ AP  SLDLK SAFDPKEKVWT+FPPEG+K+TPPHQSS+F+WKDYCP+VF
Sbjct: 421 QLGIRHAVGKQAPVVSLDLKHSAFDPKEKVWTRFPPEGTKYTPPHQSSEFKWKDYCPLVF 480

Query: 481 RTLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEV----- 540
           R+LRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTM+K+E      
Sbjct: 481 RSLRKLFKVDPADYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLR 540

Query: 541 ----------------------------------KVRFVIMGNLFCSEYTIHRRFDLKGS 600
                                             KVRFVIMGNLFCSEY+IHRRFDLKGS
Sbjct: 541 MLAAYYNHVRAFENTLVIRFYGLHCVKLTGPIQKKVRFVIMGNLFCSEYSIHRRFDLKGS 600

Query: 601 SHGRITDKPEAEIDGTTTLKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLL 660
           S GR TDKPE+EI+  T LKDLDLN+IFRLQK W+QEF RQVDKDC+FLEQERIMDYSLL
Sbjct: 601 SLGRTTDKPESEINSNTILKDLDLNFIFRLQKAWYQEFIRQVDKDCEFLEQERIMDYSLL 660

Query: 661 VGLHFQETSYREASTPESHHSGSRDPDYETDGQ-----LRADKEQ---DSARWASISLGI 718
           VG+HF+E S      P    SG+R P  E + +      RAD +Q   D  RWASI LG 
Sbjct: 661 VGIHFREASVAGELIP----SGARTPIGEFEDESAPRLSRADVDQLLSDPTRWASIRLGG 720

BLAST of HG10015727 vs. TAIR 10
Match: AT2G41210.1 (phosphatidylinositol- 4-phosphate 5-kinase 5 )

HSP 1 Score: 926.8 bits (2394), Expect = 1.1e-269
Identity = 472/794 (59.45%), Postives = 574/794 (72.29%), Query Frame = 0

Query: 1   MYKEYAGIVKAWEATVRKTQNSAKKRANSIFAAMSVAP----------ADDEPGPG---- 60
           M K+ + ++KAWE TVRKTQ  AKKRANS+F  +SVAP           DDE        
Sbjct: 1   MSKDQSYVLKAWEVTVRKTQ-QAKKRANSVFGTVSVAPHTDNDTTTDDNDDETTTNRSSL 60

Query: 61  -ELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGA 120
            E +HAE+ +PNGD+YTGQW D+ PHGHGKYLWTDGCMY+G+WY GKT+G GKF WPSGA
Sbjct: 61  EEFYHAERVLPNGDYYTGQWYDSFPHGHGKYLWTDGCMYIGDWYNGKTMGNGKFGWPSGA 120

Query: 121 TYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQ 180
           TYEG+FKSGYMDG GTYTG SGD Y+G WVMNLKHG G +++ANGD Y+GEWRRG Q+GQ
Sbjct: 121 TYEGEFKSGYMDGIGTYTGPSGDAYKGQWVMNLKHGHGVKSFANGDAYDGEWRRGLQEGQ 180

Query: 181 GRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGV 240
           G+YQW + ++YIG+WKNG I G G+ +W NGNRYDG W++G P+GNGTF+W +GS YVG 
Sbjct: 181 GKYQWSDGSYYIGEWKNGTICGKGSFVWTNGNRYDGFWDEGFPRGNGTFKWDNGSFYVGH 240

Query: 241 WSKDPTEQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMIS-W-SG 300
           WSKDP E  GT+YPS  +E  +  DP+++F   L+++ + +GER+   PSQK +S W S 
Sbjct: 241 WSKDPEEMNGTYYPS-GNEGNLEWDPKDLF-NNLSEYTICSGERVPTLPSQKKLSVWNSS 300

Query: 301 LELESPQKQTISKKGSDGNRRLRRTSVDGKVS---NSSFESLNDGSNE----ASSRNGKG 360
             +E P                RRTSVDG+VS   + +FE +N   NE     +    + 
Sbjct: 301 KRIEKP----------------RRTSVDGRVSVGVDRAFEKMNMWGNEIGEGGADMRKEL 360

Query: 361 TPQLQDLDSKGKRQ------PNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPA 420
             +L  LD++G +       P    +  ++QG TISKGH+NYELMLNLQLGIRHSVGR A
Sbjct: 361 DAELMRLDAEGLQSLKSSPVPMKLPKAGRKQGETISKGHRNYELMLNLQLGIRHSVGRQA 420

Query: 421 PATSLDLKSSAFDPKEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPA 480
           PA SLDLK SAFDPK+K+W +FP EG+K+TPPHQS++F+WKDYCP+VFR+LRKLFKVDPA
Sbjct: 421 PAASLDLKPSAFDPKDKIWRRFPREGTKYTPPHQSTEFKWKDYCPLVFRSLRKLFKVDPA 480

Query: 481 DYMLSICGNDALRELSSPGKSGSFFYLTHDDRYMIKTMRKAEV----------------- 540
           DYMLSICGNDALRELSSPGKSGSFFYLT+DDRYMIKTM+K+E                  
Sbjct: 481 DYMLSICGNDALRELSSPGKSGSFFYLTNDDRYMIKTMKKSETKVLLGMLAAYYNHVRAF 540

Query: 541 ----------------------KVRFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAE 600
                                 KVRFVIMGNLFCS+Y++HRRFDLKGSS GR TDKPE+E
Sbjct: 541 ENSLVIRFFGLHCVKLNGPTQKKVRFVIMGNLFCSKYSVHRRFDLKGSSLGRTTDKPESE 600

Query: 601 IDGTTTLKDLDLNYIFRLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQETSYRE 660
           ID  T LKDLDLN+IFRLQK W+QEF RQ+DKDC+FLEQERIMDYSLLVG+HF+E S   
Sbjct: 601 IDSNTILKDLDLNFIFRLQKAWYQEFIRQIDKDCEFLEQERIMDYSLLVGIHFREASVAG 660

Query: 661 ASTPESHHSGSRDPDYETDGQ-----LRADKEQ---DSARWASISLGINMPARAERTVRG 718
              P    SG+R P  E++ +      RA+ ++   D +RWASI LG NMPARAERT+R 
Sbjct: 661 ELIP----SGARTPIGESEEESGPRLSRAEVDELLSDPSRWASIRLGTNMPARAERTMRK 720

BLAST of HG10015727 vs. TAIR 10
Match: AT1G21980.1 (phosphatidylinositol-4-phosphate 5-kinase 1 )

HSP 1 Score: 693.3 bits (1788), Expect = 2.0e-199
Identity = 373/726 (51.38%), Postives = 464/726 (63.91%), Query Frame = 0

Query: 45  PGELHHAEKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSG 104
           P  L   EK +PNGD Y G +    PHG GKYLW DGCMY G+W +GK  GKGKFSWPSG
Sbjct: 65  PTPLVDVEKPLPNGDLYIGSFSGGFPHGSGKYLWKDGCMYEGDWKRGKASGKGKFSWPSG 124

Query: 105 ATYEGDFKSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDG 164
           ATYEG+FKSG M+G GT+TG+ GDTYRG+WV + KHG G + YANGD+YEG WRR  QDG
Sbjct: 125 ATYEGEFKSGRMEGFGTFTGADGDTYRGTWVADRKHGHGQKRYANGDFYEGTWRRNLQDG 184

Query: 165 QGRYQWKNENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVG 224
           +GRY W+N N Y G+W++G ISG G ++W NGNRY+G WE+G+PKGNG F W+DGS  VG
Sbjct: 185 RGRYVWRNGNQYTGEWRSGVISGKGLLVWPNGNRYEGLWENGIPKGNGVFTWSDGSSCVG 244

Query: 225 VWSKDPTEQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGL 284
            W++                           +  +  F     +  LI  ++K       
Sbjct: 245 AWNE---------------------------SNIMRSFFNGVEKNDLIVGNRKR------ 304

Query: 285 ELESPQKQTISKKGSDGNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLD 344
              S      S  G     R+     DG+  + + + ++  + EAS          +D  
Sbjct: 305 --SSVDSGAGSLGGEKVFPRICIWESDGEAGDITCDIID--NVEASMIYRDRISVDRDGF 364

Query: 345 SKGKRQPNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDP 404
            + K+ P      AK+ G TISKGHK Y+LMLNLQLGIR+SVG+ A     DLK + FDP
Sbjct: 365 RQFKKNPCWFNGEAKKPGQTISKGHKKYDLMLNLQLGIRYSVGKHASIVR-DLKQTDFDP 424

Query: 405 KEKVWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRE 464
           KEK WT+FPPEG+K TPPHQS DFRWKDYCP+VFR LR+LF+VDPA YML+ICGNDALRE
Sbjct: 425 KEKFWTRFPPEGTKTTPPHQSVDFRWKDYCPLVFRRLRELFQVDPAKYMLAICGNDALRE 484

Query: 465 LSSPGKSGSFFYLTHDDRYMIKTMRKAEVKV----------------------------- 524
           LSSPGKSGSFFYLT DDR+MIKT++K+EVKV                             
Sbjct: 485 LSSPGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENSLVTRFYGVHCV 544

Query: 525 --------RFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIF 584
                   RF++MGNLFCSEY I RRFDLKGSSHGR T KPE EID TTTLKDLDLN+ F
Sbjct: 545 KPVGGQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRSTAKPEGEIDETTTLKDLDLNFSF 604

Query: 585 RLQKIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQE--TSYREASTPESHHSGSRDP 644
           RLQ+ W+QE  +Q+ +DC+FLE ERIMDYSLLVG+HF++  T  +   +P    SG  D 
Sbjct: 605 RLQRNWYQELMKQIKRDCEFLEAERIMDYSLLVGVHFRDDNTGEKMGLSPFVLRSGRID- 664

Query: 645 DYETDGQLRADK-------EQD---SARWASISLGINMPARAERTVRGGEPEAQLVGEPT 704
            Y+ +  +R  +       + D   + R  SI LG NMPA+AER  R  + +    G  +
Sbjct: 665 SYQNEKFMRGCRFLEAELQDMDRILAGRKPSIRLGANMPAKAERMARRSDFDQYSSGGAS 724

Query: 705 ----GELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIF 718
               GE+Y+V+++FG+IDILQDYDI+KK+EHAYKS Q DP+SISAVDPK YSKRFRDFI 
Sbjct: 725 YPSHGEMYEVVLYFGVIDILQDYDITKKIEHAYKSLQADPASISAVDPKLYSKRFRDFIS 751

BLAST of HG10015727 vs. TAIR 10
Match: AT1G77740.1 (phosphatidylinositol-4-phosphate 5-kinase 2 )

HSP 1 Score: 684.9 bits (1766), Expect = 7.1e-197
Identity = 370/723 (51.18%), Postives = 454/723 (62.79%), Query Frame = 0

Query: 52  EKAIPNGDFYTGQWMDNMPHGHGKYLWTDGCMYVGEWYKGKTLGKGKFSWPSGATYEGDF 111
           EK +PNGD Y G +    P+G GKYLW DGCMY GEW +GK  GKGKFSWPSGATYEG+F
Sbjct: 73  EKPLPNGDLYMGTFSGGFPNGSGKYLWKDGCMYEGEWKRGKASGKGKFSWPSGATYEGEF 132

Query: 112 KSGYMDGKGTYTGSSGDTYRGSWVMNLKHGQGTQNYANGDYYEGEWRRGFQDGQGRYQWK 171
           KSG M+G GT+ G  GDTYRGSWV + K G G + YANGDYYEG WRR  QDG+GRY W 
Sbjct: 133 KSGRMEGSGTFVGVDGDTYRGSWVADRKQGHGQKRYANGDYYEGTWRRNLQDGRGRYVWM 192

Query: 172 NENHYIGQWKNGKISGNGTMIWINGNRYDGCWEDGLPKGNGTFRWADGSCYVGVWSKDPT 231
           N N Y G+W+NG I G G + W NGNRY+G WE+G+PKG+G F WADGS ++G W++   
Sbjct: 193 NGNQYTGEWRNGVICGKGVLAWPNGNRYEGQWENGVPKGSGVFTWADGSSWIGSWNESSN 252

Query: 232 EQTGTFYPSDSSETEMSSDPQEIFAEYLNDFVLSTGERILIYPSQKMISWSGLELESPQK 291
                F   + +E  +++  +             TGE+I      ++  W          
Sbjct: 253 LMRNFFDGIEKNELIVATRKRSSVDSGAGSL---TGEKIF----PRICIWES-------- 312

Query: 292 QTISKKGSDGNRRLRRTSVDGKVSNSSFESLNDGSNEASSRNGKGTPQLQDLDSKGKRQ- 351
                              DG+  + + + +++       R+         +D  G RQ 
Sbjct: 313 -------------------DGEAGDITCDIVDNVEASVIYRD------RISIDKDGFRQF 372

Query: 352 ---PNLRFQPAKRQGVTISKGHKNYELMLNLQLGIRHSVGRPAPATSLDLKSSAFDPKEK 411
              P      AK+ G TISKGHK Y+LMLNLQ GIR+SVG+ A     DLK S FDP EK
Sbjct: 373 RKNPCCFSGEAKKPGETISKGHKKYDLMLNLQHGIRYSVGKHASVVR-DLKQSDFDPSEK 432

Query: 412 VWTKFPPEGSKHTPPHQSSDFRWKDYCPVVFRTLRKLFKVDPADYMLSICGNDALRELSS 471
            WT+FPPEGSK TPPH S DFRWKDYCP+VFR LR+LF VDPADYML+ICGNDALRELSS
Sbjct: 433 FWTRFPPEGSKTTPPHLSVDFRWKDYCPLVFRRLRELFTVDPADYMLAICGNDALRELSS 492

Query: 472 PGKSGSFFYLTHDDRYMIKTMRKAEVKV-------------------------------- 531
           PGKSGSFFYLT DDR+MIKT++K+EVKV                                
Sbjct: 493 PGKSGSFFYLTQDDRFMIKTVKKSEVKVLLRMLPSYYKHVCQYENTLVTRFYGVHCIKPV 552

Query: 532 -----RFVIMGNLFCSEYTIHRRFDLKGSSHGRITDKPEAEIDGTTTLKDLDLNYIFRLQ 591
                RF++MGNLFCSEY I RRFDLKGSSHGR T KPE EID TTTLKDLDLN+ FRLQ
Sbjct: 553 GGQKTRFIVMGNLFCSEYRIQRRFDLKGSSHGRYTSKPEGEIDETTTLKDLDLNFAFRLQ 612

Query: 592 KIWFQEFCRQVDKDCDFLEQERIMDYSLLVGLHFQE--TSYREASTPESHHSGSRDPDYE 651
           + W+QE   Q+ +DC+FLE ERIMDYSLLVG+HF++  T  +   +P    SG  +  Y+
Sbjct: 613 RNWYQELMTQIKRDCEFLEAERIMDYSLLVGVHFRDDNTGDKMGLSPFVLRSGKIE-SYQ 672

Query: 652 TDGQLRADK-------EQD---SARWASISLGINMPARAERTVRGGEPEAQLVG----EP 711
           ++  +R  +       + D   + R   I LG NMPARAER  R  + +    G    + 
Sbjct: 673 SEKFMRGCRFLEAELQDMDRILAGRKPLIRLGANMPARAERMARRSDYDQYSSGGTNYQS 732

Query: 712 TGELYDVIIFFGIIDILQDYDISKKLEHAYKSFQYDPSSISAVDPKQYSKRFRDFIFRIF 718
            GE+Y+V+++FGIIDILQDYDISKK+EHAYKS Q DP+SISAVDPK YS+RFRDFI RIF
Sbjct: 733 HGEVYEVVLYFGIIDILQDYDISKKIEHAYKSLQADPASISAVDPKLYSRRFRDFISRIF 753