HG10015724 (gene) Bottle gourd (Hangzhou Gourd) v1

BLAST of HG10015724 vs. NCBI nr
Match: XP_038892997.1 (protein NRT1/ PTR FAMILY 5.4 [Benincasa hispida])

HSP 1 Score: 684.9 bits (1766), Expect = 5.5e-193
Identity = 393/587 (66.95%), Postives = 424/587 (72.23%), Query Frame = 0

Query: 4   NPIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVF 63
           NP+I L  KF   PTASN PTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVF
Sbjct: 9   NPVINLSNKFLDRPTASNRPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVF 68

Query: 64  HEPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLI 123
           HEP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASLIYCLGMVLLT+SAT+I
Sbjct: 69  HEPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIYCLGMVLLTISATVI 128

Query: 124 GAPHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVG 183
           GA HRKP+FF ALYILS+GEGGHRPCVQTFAADQFDE+SPE+RKKKSSFFNWWYVGLVVG
Sbjct: 129 GADHRKPVFFLALYILSVGEGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVVG 188

Query: 184 STFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVV 243
           STFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVV
Sbjct: 189 STFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVV 248

Query: 244 AAARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHKARLH 303
           AA RKWRVDETRHEWRVCYDE+NHAKNE E Q+KLMT V TNQF        IL KA L 
Sbjct: 249 AAVRKWRVDETRHEWRVCYDEENHAKNEGESQYKLMTLVRTNQF-------RILDKAALI 308

Query: 304 HAPLRGPPLSAPAGISSRCRRPHHPSHRPLLRP------RFCPRRQKLYRPPLRHNSATK 363
                      P  +S+  R          L P       F   + +++    +  S   
Sbjct: 309 DEEDEARKKRNPWRLST-VREVEEVKLVVRLIPVWVSCLMFAVVQAQMHTFFTKQGSTML 368

Query: 364 NRNGP-IHLHP-------------------------------HHGRL----RAGRG---- 423
              GP   L P                               HH  +    R G G    
Sbjct: 369 RSVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTAHHSGITVLQRIGMGLFIS 428

Query: 424 ---QKGNGRCRAR----PHRHAESDSSN-----------------GVSDAFAIIGLQELF 483
                 +    A+      +H  +D+                   GVSDAFAIIGLQELF
Sbjct: 429 ILTMGVSALVEAKRVTIAAQHGLTDTPKVTIPMTIWWLIPQYMLCGVSDAFAIIGLQELF 488

Query: 484 YDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLA 521
           YDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGS G WLEDNLNRS+LHYFYWVLA
Sbjct: 489 YDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSGGSWLEDNLNRSNLHYFYWVLA 548

BLAST of HG10015724 vs. NCBI nr
Match: KAA0034758.1 (protein NRT1/ PTR FAMILY 5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 657.5 bits (1695), Expect = 9.4e-185
Identity = 379/587 (64.57%), Postives = 418/587 (71.21%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASN+PT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASL++  GMV+LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
             HRK +FF  LYILS+G+GGHRPCVQTFAADQF+E +PE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHK---AR 304
           AARKW VDETRHEWRVCY+EDNHAKNE EGQH LMT   TNQFS     A ++ K   AR
Sbjct: 251 AARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFSRILDKATLIDKEDEAR 310

Query: 305 LHHAPLRGPPLS-----------APAGISS----------------------RCRRPHH- 364
               P R   +             P  +S                       R   PH  
Sbjct: 311 KKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQ 370

Query: 365 --PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHGRLR 424
             P+          LL   F  R   ++ P  R     H+  T  +   I +      L 
Sbjct: 371 LPPASLQGVVGLTILLTVLFYDR---VFVPAARNFTGHHSGITVLQR--IGMGLFISILT 430

Query: 425 AGRGQKGNGRCRARPHRHAESDSSN-----------------GVSDAFAIIGLQELFYDQ 484
            G       +       H  SD+                   GVSDAFAI+GLQELFYDQ
Sbjct: 431 MGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELFYDQ 490

Query: 485 MPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAALS 521
           MPQ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWL+DNLNRS+LHYFYWVLAALS
Sbjct: 491 MPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVLAALS 550

BLAST of HG10015724 vs. NCBI nr
Match: XP_031740965.1 (protein NRT1/ PTR FAMILY 5.4 isoform X1 [Cucumis sativus])

HSP 1 Score: 652.1 bits (1681), Expect = 4.0e-183
Identity = 373/587 (63.54%), Postives = 415/587 (70.70%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASNSPT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASLI+ +GM++LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
              RK +FF  LYILS+G+GGHRPCVQTFAADQFDE+SPE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHK---AR 304
           AARKWRVDETR+ WR+CY+EDN AKN+ EG+H LMT   TNQFS     A ++ K   AR
Sbjct: 251 AARKWRVDETRNGWRICYEEDNRAKNDAEGEHNLMTLARTNQFSRILDKATLIDKEDEAR 310

Query: 305 LHHAPLRGPPLSAPAGISSRCRRPHHPSHRPLLRPRFCPRRQKLYRPPLRHNSATKNRNG 364
               P R   +     +    R    P     L   F   + +++    +  S      G
Sbjct: 311 KKRDPWRLSTVEEVEEVKLVVRL--IPVWVSCL--MFAVVQAQIHTFFTKQGSTMLRSVG 370

Query: 365 P-IHLHP-------------------------------HHGRL----RAGRG-------Q 424
           P   L P                               HH  +    R G G        
Sbjct: 371 PHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISIFTM 430

Query: 425 KGNGRCRAR----PHRHAESDSSN-----------------GVSDAFAIIGLQELFYDQM 484
             +    A+       H  SD+                   GVSDAFAIIGLQELFYDQM
Sbjct: 431 GVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFYDQM 490

Query: 485 PQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAALSA 521
           P+ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWLEDNLNRS+LHYFYWVLAALSA
Sbjct: 491 PEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVLAALSA 550

BLAST of HG10015724 vs. NCBI nr
Match: XP_008446958.1 (PREDICTED: protein NRT1/ PTR FAMILY 5.4 [Cucumis melo] >TYK09312.1 protein NRT1/ PTR FAMILY 5.4 [Cucumis melo var. makuwa])

HSP 1 Score: 651.7 bits (1680), Expect = 5.2e-183
Identity = 378/587 (64.40%), Postives = 417/587 (71.04%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASN+PT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASL++  GMV+LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
             HRK +FF  LYILS+G+GGHRPCVQTFAADQF+E +PE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHK---AR 304
           AARKW VDETRHEWRVCY+EDNHAKNE EGQH LMT   TNQF      A ++ K   AR
Sbjct: 251 AARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFR-ILDKATLIDKEDEAR 310

Query: 305 LHHAPLRGPPLS-----------APAGISS----------------------RCRRPHH- 364
               P R   +             P  +S                       R   PH  
Sbjct: 311 KKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQ 370

Query: 365 --PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHGRLR 424
             P+          LL   F  R   ++ P  R     H+  T  +   I +      L 
Sbjct: 371 LPPASLQGVVGLTILLTVLFYDR---VFVPAARNFTGHHSGITVLQR--IGMGLFISILT 430

Query: 425 AGRGQKGNGRCRARPHRHAESDSSN-----------------GVSDAFAIIGLQELFYDQ 484
            G       +       H  SD+                   GVSDAFAI+GLQELFYDQ
Sbjct: 431 MGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELFYDQ 490

Query: 485 MPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAALS 521
           MPQ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWL+DNLNRS+LHYFYWVLAALS
Sbjct: 491 MPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVLAALS 550

BLAST of HG10015724 vs. NCBI nr
Match: XP_011655920.1 (protein NRT1/ PTR FAMILY 5.4 isoform X2 [Cucumis sativus] >KAE8648867.1 hypothetical protein Csa_008874 [Cucumis sativus])

HSP 1 Score: 645.6 bits (1664), Expect = 3.7e-181
Identity = 372/590 (63.05%), Postives = 412/590 (69.83%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASNSPT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNSPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASLI+ +GM++LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLIFFIGMMVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
              RK +FF  LYILS+G+GGHRPCVQTFAADQFDE+SPE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DNQRKAVFFLGLYILSVGQGGHRPCVQTFAADQFDEESPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHKARLHH 304
           AARKWRVDETR+ WR+CY+EDN AKN+ EG+H LMT   TNQF        IL KA L  
Sbjct: 251 AARKWRVDETRNGWRICYEEDNRAKNDAEGEHNLMTLARTNQF-------RILDKATLID 310

Query: 305 APLRGPPLSAPAGISSRCRRPHHPSHRPLLRP------RFCPRRQKLYRPPLRHNSATKN 364
                     P  +S+             L P       F   + +++    +  S    
Sbjct: 311 KEDEARKKRDPWRLST-VEEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLR 370

Query: 365 RNGP-IHLHP-------------------------------HHGRL----RAGRG----- 424
             GP   L P                               HH  +    R G G     
Sbjct: 371 SVGPHFQLPPASLQGVVGLTILLTVLFYDRVFVPAARNFTGHHSGITVLQRIGMGLFISI 430

Query: 425 --QKGNGRCRAR----PHRHAESDSSN-----------------GVSDAFAIIGLQELFY 484
                +    A+       H  SD+                   GVSDAFAIIGLQELFY
Sbjct: 431 FTMGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIIGLQELFY 490

Query: 485 DQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAA 521
           DQMP+ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWLEDNLNRS+LHYFYWVLAA
Sbjct: 491 DQMPEFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLEDNLNRSNLHYFYWVLAA 550

BLAST of HG10015724 vs. ExPASy Swiss-Prot
Match: Q9M1I2 (Protein NRT1/ PTR FAMILY 5.4 OS=Arabidopsis thaliana OX=3702 GN=NPF5.4 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 2.8e-99
Identity = 211/546 (38.64%), Postives = 313/546 (57.33%), Query Frame = 0

Query: 29  GGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSALFPLLG 88
           GGW AA+FII VE+AE+FAF GL+SNLI + T    +    AAK +N W+GVS +FP+LG
Sbjct: 14  GGWNAALFIIVVEIAERFAFYGLASNLITFLTNELGQSTATAAKNINTWIGVSCMFPILG 73

Query: 89  AFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIGAPHRKPLFFFALYILSLGEGGHRP 148
           AF+ADS+LGRFKT+++ S IY LG+V+L +S T++    R+ +FF ALY++++GEGGH+P
Sbjct: 74  AFLADSILGRFKTVLLTSFIYLLGIVMLPLSVTVVARRMREKVFFMALYVMAVGEGGHKP 133

Query: 149 CVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFLVIYVQDNIGWGLSFGILA 208
           CV TFAADQF E + E++  K+SFFN+WY+ +V+ S+ AV  +I++Q+ + W L F I+A
Sbjct: 134 CVMTFAADQFGEANAEEKAAKTSFFNYWYMAIVLASSIAVLALIFIQERVSWSLGFSIIA 193

Query: 209 GVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVAAARKWRVDETRHEWRVCYDEDNHA 268
           G +  AI+IFL G+ KYR+QVPVGSP T++AQV+VAA +KWR+  TRH + +CY+E++  
Sbjct: 194 GSVVIAIVIFLIGIPKYRKQVPVGSPFTRVAQVMVAALKKWRLSSTRHHYGLCYEEEDEH 253

Query: 269 KNEEEGQHKLMTWVPTNQFSGASSDAHILHKA----RLHHAPLRGPPLSAPAGISSRCRR 328
           K E    +++     TNQF         L KA     + H   R P            + 
Sbjct: 254 KLESTNSNQVYLLARTNQF-------RFLDKATIIDEIDHNKNRNPWRLCTVNQVEEVKL 313

Query: 329 PHHPSHRPLLRPRFCPRRQKLYRPPLRHNSATKNRNG----------------------P 388
                   +    FC    +L    L+  S      G                      P
Sbjct: 314 ILRLIPIWISLIMFCATLTQLNTFFLKQGSMMDRTIGNHFTIPPAAFQSIVGVTILILIP 373

Query: 389 IH----------LHPHHGRL----RAGRG-------------QKGNGRCRARPHRHAESD 448
           ++          +  HH  +    R G G              +      AR H   +S 
Sbjct: 374 LYDRVFVPMVRKITNHHSGITSLQRIGVGLFVATFNMVICGLVEAKRLKVARDHGLIDSP 433

Query: 449 SS---------------NGVSDAFAIIGLQELFYDQMPQSMRSLGAAAYISIIGVGNFLS 504
                             G+ D F I+G+QELFYDQMP++MRS+GAA +IS++GVG+F+S
Sbjct: 434 KEVVPMSSLWLLPQYILVGIGDVFTIVGMQELFYDQMPETMRSIGAAIFISVVGVGSFVS 493

BLAST of HG10015724 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 8.0e-70
Identity = 194/568 (34.15%), Postives = 280/568 (49.30%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           P++ +   + + P   +S      GGW++A FII VEVAE+FA+ G+SSNLI Y T    
Sbjct: 13  PLLAVTVDYRNKPAVKSS-----SGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLG 72

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           +    AA  VN W G ++L PLLGAF+ADS LGRF+TI+ AS +Y +G+ +LT+SA +  
Sbjct: 73  QSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPS 132

Query: 125 -----------APHRKPL-FFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSF 184
                      +P  + + FF ALY+++L +GGH+PCVQ F ADQFDE  PE+ K KSSF
Sbjct: 133 DCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSF 192

Query: 185 FNWWYVGLVVGSTFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQV--P 244
           FNWWY G+  G+   ++++ Y+QDN+ W L FGI    +  A+++ L G   YR  +   
Sbjct: 193 FNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRRE 252

Query: 245 VGSPLTKIAQVVVAAARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFS-- 304
             SP  +I  V VAA + W V             D  A  E  G   L++   + QFS  
Sbjct: 253 DQSPFVRIGNVYVAAVKNWSVSAL----------DVAAAEERLG---LVSCSSSQQFSFL 312

Query: 305 ---------GASSDAHILHKARLHHAPL------------RGPPLSAPAGIS-SRCRRPH 364
                      S D     K+ L  AP+            + P      G +  R   P 
Sbjct: 313 NKALVAKNGSCSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPG 372

Query: 365 HPSHRPLLRP-------RFCPRRQKLYRPPLRHNSATKNRNGPIHLHPHHGRLRAGRG-- 424
           +      L+         F P   ++  P  R  S T    G   L       R G G  
Sbjct: 373 YKISPATLQSFISLSIVIFIPIYDRVLIPIAR--SFTHKPGGITMLQ------RIGTGIF 432

Query: 425 -----------QKGNGRCRARPHRHAESDSSN---------------GVSDAFAIIGLQE 484
                       +      A  +   +S  +                G++D FA++GLQE
Sbjct: 433 LSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQE 492

Query: 485 LFYDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGS----RGRWLEDNLNRSSLHY 496
            FYDQ+P  +RS+G A Y+SI G+GNFLSS +ISI++  +    +  W  +NLN++ L Y
Sbjct: 493 FFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDY 552

BLAST of HG10015724 vs. ExPASy Swiss-Prot
Match: Q9M331 (Protein NRT1/ PTR FAMILY 5.7 OS=Arabidopsis thaliana OX=3702 GN=NPF5.7 PE=2 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-65
Identity = 188/575 (32.70%), Postives = 280/575 (48.70%), Query Frame = 0

Query: 23  PTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSA 82
           P     G W+AA+FII +E +E+ ++ G+S+NL++Y TT+ H+ +  A K  N W GV+ 
Sbjct: 33  PLRAQTGAWRAALFIIGIEFSERLSYFGISTNLVVYLTTILHQDLKMAVKNTNYWSGVTT 92

Query: 83  LFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG--APH----------RKP 142
           L PLLG F+AD+ LGR+ T+++A+ IY +G++LLT+S  + G  A H           + 
Sbjct: 93  LMPLLGGFVADAYLGRYGTVLLATTIYLMGLILLTLSWFIPGLKACHEDMCVEPRKAHEI 152

Query: 143 LFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFL 202
            FF A+Y++S+G GGH+P +++F ADQF++  PE+RK K S+FNWW  GL  G   AV +
Sbjct: 153 AFFIAIYLISIGTGGHKPSLESFGADQFEDGHPEERKMKMSYFNWWNAGLCAGILTAVTV 212

Query: 203 VIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVAAARKWR 262
           ++Y++D IGWG++  IL  V+A +  IF  G   YR + P GSPLT + QV VAA  K  
Sbjct: 213 IVYIEDRIGWGVASIILTIVMATSFFIFRIGKPFYRYRAPSGSPLTPMLQVFVAAIAKRN 272

Query: 263 V----------DETRHEW------------------RVCYDEDNHAKNEEEGQHKLMT-- 322
           +          + T  E+                   V  D + + K E++   +L T  
Sbjct: 273 LPCPSDSSLLHELTNEEYTKGRLLSSSKNLKFLDKAAVIEDRNENTKAEKQSPWRLATVT 332

Query: 323 --------------WVPTNQFS--GASSDAHILHKARLHHAPLRGPP-LSAPAGISSRCR 382
                         W  T  F      S    + +A +    + G   +  PA + S   
Sbjct: 333 KVEEVKLLINMIPIWFFTLAFGVCATQSSTLFIKQAIIMDRHITGTSFIVPPASLFSLI- 392

Query: 383 RPHHPSHRPLLRPRFCPRRQKLYRPPLRHNSATKNRNGPIHLHP---------------- 442
                    L         +KL  P LR   AT N  G   L                  
Sbjct: 393 --------ALSIIITVTIYEKLLVPLLRR--ATGNERGISILQRIGVGMVFSLFAMIIAA 452

Query: 443 --HHGRLRAGRGQKGNGRCRARPHRHAESDSSNGVSDAFAIIGLQELFYDQMPQSMRSLG 502
                RL   +    N          A      GV+DAF ++GLQE FYDQ+P SMRSLG
Sbjct: 453 LIEKKRLDYAKEHHMNKTMTLSAIWLAPQFLVLGVADAFTLVGLQEYFYDQVPDSMRSLG 512

Query: 503 AAAYISIIGVGNFLSSAVISI----VQAGSRGRWLEDNLNRSSLHYFYWVLAALSALNLC 513
            A Y+S++G  +F+++ +I++     +  S   W   +LN S L  FYW+LAAL+A N+C
Sbjct: 513 IAFYLSVLGAASFVNNLLITVSDHLAEEISGKGWFGKDLNSSRLDRFYWMLAALTAANIC 572

BLAST of HG10015724 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 251.1 bits (640), Expect = 2.7e-65
Identity = 172/547 (31.44%), Postives = 268/547 (48.99%), Query Frame = 0

Query: 29  GGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSALFPLLG 88
           G W+AA+FII VEVAE+FA+ G+ SNLI Y T    E    AA  VN W G++ L P+LG
Sbjct: 31  GRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAANVNAWSGIATLLPVLG 90

Query: 89  AFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLI---------GAPHRKPLFFFALYIL 148
           AF+AD+ LGR++TIII+SLIY LG+  LT+SA LI          +     LFFF+LY++
Sbjct: 91  AFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSSTSSFLNVLFFFSLYLV 150

Query: 149 SLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFLVIYVQDNIG 208
           ++G+ GH+PCVQ F ADQFDE   +++  +SSFFNWWY+ L  G  FA+ +V+Y+Q+   
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFS 210

Query: 209 WGLSFGILAGVLAAAIIIFLAGVKKY----RRQVPVGSPLTKIAQVVVAAARKWRVD--- 268
           W   FGI    +  ++++F++G + Y    RR     +P T+I +V   A +  R+    
Sbjct: 211 WAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSSD 270

Query: 269 --------ETRHEWRVCYDE-----------DNHAKNEEEGQ-----HKLMTW------- 328
                    T  E +  +++           +N +K+ +          +  W       
Sbjct: 271 LCKVELEANTSPEKQSFFNKALLVPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYA 330

Query: 329 VPTNQF------SGASSDAHILHKARLHHAPLRGPPLSAPAGISSRCRRPHHPSHRPLLR 388
           +P  Q+       G + D  IL   ++       PP S    I             P+  
Sbjct: 331 IPYAQYMTFFTKQGVTMDRTILPGVKI-------PPASLQVFIGISI-----VLFVPIYD 390

Query: 389 PRFCPRRQKLYRPPLRHNSATKNRNG--------PIHLHPHHGRLRAGRGQKGNGRCRAR 448
             F P  + + + P    +  +   G         I       RL             A+
Sbjct: 391 RVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLET-----------AK 450

Query: 449 PHRHAESDSSN---------------GVSDAFAIIGLQELFYDQMPQSMRSLGAAAYISI 496
            H   +   +                G++D + ++G+QE FY Q+P  +RS+G A Y+S 
Sbjct: 451 EHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLSA 510

BLAST of HG10015724 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 247.7 bits (631), Expect = 2.9e-64
Identity = 177/540 (32.78%), Postives = 265/540 (49.07%), Query Frame = 0

Query: 29  GGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSALFPLLG 88
           G W+AA FII VEVAE+FA  G+ SNLI Y T    +    AA  VN W G+S + PLLG
Sbjct: 31  GRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAVAAANVNAWSGISTILPLLG 90

Query: 89  AFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLI---------GAPHRKPLFFFALYIL 148
           AF+AD+ LGR+ TIIIAS IY LG+  LT+SA LI          +     LFFF+LY++
Sbjct: 91  AFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEVTSSPSSFLNALFFFSLYLV 150

Query: 149 SLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFLVIYVQDNIG 208
           ++G+ GH+PCVQ F ADQFDE +P++   +SSFFNWWY+ +  G   A+ +V+Y+Q+N+ 
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVS 210

Query: 209 WGLSFGILAGVLAAAIIIFLAGVKKYR----RQVPVGSPLTKIAQVVVAAARKWRVDETR 268
           W L FGI    +  ++++F+ G K YR    RQ    +P T+I +V   A +  R++ + 
Sbjct: 211 WALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSS- 270

Query: 269 HEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHKAR-------------- 328
               +C  E   A   +E   +L            S +  +  K+R              
Sbjct: 271 ---DLCKVELIEANRSQESPEELSFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPV 330

Query: 329 ----LHHAPLRGPPLSAPAGISSRCRRPHHP-------SHRPLLR---PRFCPRRQKLYR 388
               L +A      ++          R   P       S + L+      F P   ++  
Sbjct: 331 WLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLV 390

Query: 389 PPLRHNSATKNRNGPIHLHPHHGRLRAGRGQ-------------KGNGRCRARPHRHAES 448
           P  R  S TK+  G   L       R G G              +      A+ +   + 
Sbjct: 391 PIGR--SITKDPCGITTLK------RIGTGMVLATLTMVVAALVESKRLETAKEYGLIDQ 450

Query: 449 DSSN---------------GVSDAFAIIGLQELFYDQMPQSMRSLGAAAYISIIGVGNFL 496
             +                G++D   ++G+QE FY Q+P  +RSLG A Y+S +GVG+ L
Sbjct: 451 PKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIYLSAMGVGSLL 510

BLAST of HG10015724 vs. ExPASy TrEMBL
Match: A0A5A7SVT8 (Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007820 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 4.6e-185
Identity = 379/587 (64.57%), Postives = 418/587 (71.21%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASN+PT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASL++  GMV+LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
             HRK +FF  LYILS+G+GGHRPCVQTFAADQF+E +PE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHK---AR 304
           AARKW VDETRHEWRVCY+EDNHAKNE EGQH LMT   TNQFS     A ++ K   AR
Sbjct: 251 AARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFSRILDKATLIDKEDEAR 310

Query: 305 LHHAPLRGPPLS-----------APAGISS----------------------RCRRPHH- 364
               P R   +             P  +S                       R   PH  
Sbjct: 311 KKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQ 370

Query: 365 --PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHGRLR 424
             P+          LL   F  R   ++ P  R     H+  T  +   I +      L 
Sbjct: 371 LPPASLQGVVGLTILLTVLFYDR---VFVPAARNFTGHHSGITVLQR--IGMGLFISILT 430

Query: 425 AGRGQKGNGRCRARPHRHAESDSSN-----------------GVSDAFAIIGLQELFYDQ 484
            G       +       H  SD+                   GVSDAFAI+GLQELFYDQ
Sbjct: 431 MGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELFYDQ 490

Query: 485 MPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAALS 521
           MPQ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWL+DNLNRS+LHYFYWVLAALS
Sbjct: 491 MPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVLAALS 550

BLAST of HG10015724 vs. ExPASy TrEMBL
Match: A0A5D3CDG7 (Protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G003090 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 2.5e-183
Identity = 378/587 (64.40%), Postives = 417/587 (71.04%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASN+PT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASL++  GMV+LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
             HRK +FF  LYILS+G+GGHRPCVQTFAADQF+E +PE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHK---AR 304
           AARKW VDETRHEWRVCY+EDNHAKNE EGQH LMT   TNQF      A ++ K   AR
Sbjct: 251 AARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFR-ILDKATLIDKEDEAR 310

Query: 305 LHHAPLRGPPLS-----------APAGISS----------------------RCRRPHH- 364
               P R   +             P  +S                       R   PH  
Sbjct: 311 KKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQ 370

Query: 365 --PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHGRLR 424
             P+          LL   F  R   ++ P  R     H+  T  +   I +      L 
Sbjct: 371 LPPASLQGVVGLTILLTVLFYDR---VFVPAARNFTGHHSGITVLQR--IGMGLFISILT 430

Query: 425 AGRGQKGNGRCRARPHRHAESDSSN-----------------GVSDAFAIIGLQELFYDQ 484
            G       +       H  SD+                   GVSDAFAI+GLQELFYDQ
Sbjct: 431 MGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELFYDQ 490

Query: 485 MPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAALS 521
           MPQ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWL+DNLNRS+LHYFYWVLAALS
Sbjct: 491 MPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVLAALS 550

BLAST of HG10015724 vs. ExPASy TrEMBL
Match: A0A1S3BFS1 (protein NRT1/ PTR FAMILY 5.4 OS=Cucumis melo OX=3656 GN=LOC103489513 PE=3 SV=1)

HSP 1 Score: 651.7 bits (1680), Expect = 2.5e-183
Identity = 378/587 (64.40%), Postives = 417/587 (71.04%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           PII LP KFP HPTASN+PT+ PGGGWKAAIFIIFVEVAEQFA IGLSSNLIMYFTTVFH
Sbjct: 11  PIINLPNKFPDHPTASNTPTVRPGGGWKAAIFIIFVEVAEQFASIGLSSNLIMYFTTVFH 70

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP+G AAK+VNNWVGVSA+FPLLGAF+ADSLLGRFKTIIIASL++  GMV+LTVSAT++G
Sbjct: 71  EPLGVAAKQVNNWVGVSAVFPLLGAFVADSLLGRFKTIIIASLVFFFGMVVLTVSATVVG 130

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
             HRK +FF  LYILS+G+GGHRPCVQTFAADQF+E +PE+RKKKSSFFNWWYVGLV GS
Sbjct: 131 DDHRKAVFFLGLYILSVGQGGHRPCVQTFAADQFEERTPEERKKKSSFFNWWYVGLVGGS 190

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           TFAVF+VIYVQDNIGWGLSFGILAGVLAAAII+FLAGVKKYRRQVPVGSPLT+IAQVVVA
Sbjct: 191 TFAVFVVIYVQDNIGWGLSFGILAGVLAAAIILFLAGVKKYRRQVPVGSPLTRIAQVVVA 250

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHK---AR 304
           AARKW VDETRHEWRVCY+EDNHAKNE EGQH LMT   TNQF      A ++ K   AR
Sbjct: 251 AARKWGVDETRHEWRVCYEEDNHAKNEGEGQHNLMTLARTNQFR-ILDKATLIDKEDEAR 310

Query: 305 LHHAPLRGPPLS-----------APAGISS----------------------RCRRPHH- 364
               P R   +             P  +S                       R   PH  
Sbjct: 311 KKRDPWRLSTVGEVEEVKLVVRLIPVWVSCLMFAVVQAQIHTFFTKQGSTMLRSVGPHFQ 370

Query: 365 --PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHGRLR 424
             P+          LL   F  R   ++ P  R     H+  T  +   I +      L 
Sbjct: 371 LPPASLQGVVGLTILLTVLFYDR---VFVPAARNFTGHHSGITVLQR--IGMGLFISILT 430

Query: 425 AGRGQKGNGRCRARPHRHAESDSSN-----------------GVSDAFAIIGLQELFYDQ 484
            G       +       H  SD+                   GVSDAFAI+GLQELFYDQ
Sbjct: 431 MGVSALVEAKRVTIAAEHGLSDTPKATVPMTIWWLIPQYMLCGVSDAFAIVGLQELFYDQ 490

Query: 485 MPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAALS 521
           MPQ MRSLGAAAYISIIGVGNFLSSA+IS+VQAGS GRWL+DNLNRS+LHYFYWVLAALS
Sbjct: 491 MPQFMRSLGAAAYISIIGVGNFLSSAIISVVQAGSGGRWLDDNLNRSNLHYFYWVLAALS 550

BLAST of HG10015724 vs. ExPASy TrEMBL
Match: A0A6J1GWF8 (protein NRT1/ PTR FAMILY 5.4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458199 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 3.5e-169
Identity = 346/585 (59.15%), Postives = 398/585 (68.03%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           P++ LP K P H   S+ P+ PP GGWKAAIF+IFVEVAEQFAFIGLSSNLIMYFTTVFH
Sbjct: 9   PLLHLPTKLPDHRNLSDRPSQPPAGGWKAAIFVIFVEVAEQFAFIGLSSNLIMYFTTVFH 68

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP   AAK VNNW GVSA+FP+LGAF+ADSLLGRFKTII +SLIYCLGMVLLT+SAT+IG
Sbjct: 69  EPTATAAKMVNNWSGVSAVFPILGAFVADSLLGRFKTIIFSSLIYCLGMVLLTLSATVIG 128

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
           APHRKP+FFFALYILS+GEGGHRPCVQTFAADQFDE++PE RK+KSSFFNWWYVGLVVGS
Sbjct: 129 APHRKPVFFFALYILSVGEGGHRPCVQTFAADQFDEETPEQRKRKSSFFNWWYVGLVVGS 188

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           T AVFLVIYVQDNIGWGLSFGILAGVLAAA+++FL GVK YRR +PVGSP+T+IAQVVVA
Sbjct: 189 TLAVFLVIYVQDNIGWGLSFGILAGVLAAALLLFLCGVKVYRRHIPVGSPMTRIAQVVVA 248

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHIL---HKAR 304
           AARKWRVD TR EWRVCY+ED+HAKNE+EGQHK MT    +QF      A ++    KAR
Sbjct: 249 AARKWRVDNTRQEWRVCYEEDSHAKNEDEGQHKPMTLDRRSQFGRILDKATLIDDEDKAR 308

Query: 305 LHHAPLRGPPLSAPAGISS------------------------------------RCRRP 364
               P R   LS  A +                                      R   P
Sbjct: 309 KKRDPWR---LSTVAEVEEVKMLGRLIPVWFSCLMFAVVQAQIHTFFTKQGSTMLRSIGP 368

Query: 365 HH---PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHG 424
           H    P+          LL   F  R   ++ P  R     H+  T  +   I L     
Sbjct: 369 HFQIPPASLQGVVGLTILLTVLFYDR---VFVPSARKFTGHHSGITVLQRIGIGLFISIL 428

Query: 425 RLRAGRGQKGNGRCRARPHRHAESDSSN---------------GVSDAFAIIGLQELFYD 484
            + A    +      A  H   ++                   GVSDAFA++GLQELFYD
Sbjct: 429 NMVASALVEAKRVAVAAEHGLIDTPKVTVPMTIWWLIPQYMLCGVSDAFAVVGLQELFYD 488

Query: 485 QMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAAL 521
           QMP+SMRS+G+AAYIS+IG+GNFLS+A+IS VQA SR +WL DNLNRS L YFYWVLA L
Sbjct: 489 QMPESMRSIGSAAYISVIGIGNFLSTAIISAVQAASRHKWLVDNLNRSKLQYFYWVLAGL 548

BLAST of HG10015724 vs. ExPASy TrEMBL
Match: A0A6J1GWY8 (protein NRT1/ PTR FAMILY 5.4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111458199 PE=3 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 1.0e-168
Identity = 347/585 (59.32%), Postives = 399/585 (68.21%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           P++ LP K P H   S+ P+ PP GGWKAAIF+IFVEVAEQFAFIGLSSNLIMYFTTVFH
Sbjct: 9   PLLHLPTKLPDHRNLSDRPSQPPAGGWKAAIFVIFVEVAEQFAFIGLSSNLIMYFTTVFH 68

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           EP   AAK VNNW GVSA+FP+LGAF+ADSLLGRFKTII +SLIYCLGMVLLT+SAT+IG
Sbjct: 69  EPTATAAKMVNNWSGVSAVFPILGAFVADSLLGRFKTIIFSSLIYCLGMVLLTLSATVIG 128

Query: 125 APHRKPLFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGS 184
           APHRKP+FFFALYILS+GEGGHRPCVQTFAADQFDE++PE RK+KSSFFNWWYVGLVVGS
Sbjct: 129 APHRKPVFFFALYILSVGEGGHRPCVQTFAADQFDEETPEQRKRKSSFFNWWYVGLVVGS 188

Query: 185 TFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVA 244
           T AVFLVIYVQDNIGWGLSFGILAGVLAAA+++FL GVK YRR +PVGSP+T+IAQVVVA
Sbjct: 189 TLAVFLVIYVQDNIGWGLSFGILAGVLAAALLLFLCGVKVYRRHIPVGSPMTRIAQVVVA 248

Query: 245 AARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHIL---HKAR 304
           AARKWRVD TR EWRVCY+ED+HAKNE+EGQHK MT    +QF G    A ++    KAR
Sbjct: 249 AARKWRVDNTRQEWRVCYEEDSHAKNEDEGQHKPMTLDRRSQF-GILDKATLIDDEDKAR 308

Query: 305 LHHAPLRGPPLSAPAGISS------------------------------------RCRRP 364
               P R   LS  A +                                      R   P
Sbjct: 309 KKRDPWR---LSTVAEVEEVKMLGRLIPVWFSCLMFAVVQAQIHTFFTKQGSTMLRSIGP 368

Query: 365 HH---PSHRP-------LLRPRFCPRRQKLYRPPLR-----HNSATKNRNGPIHLHPHHG 424
           H    P+          LL   F  R   ++ P  R     H+  T  +   I L     
Sbjct: 369 HFQIPPASLQGVVGLTILLTVLFYDR---VFVPSARKFTGHHSGITVLQRIGIGLFISIL 428

Query: 425 RLRAGRGQKGNGRCRARPHRHAESDSSN---------------GVSDAFAIIGLQELFYD 484
            + A    +      A  H   ++                   GVSDAFA++GLQELFYD
Sbjct: 429 NMVASALVEAKRVAVAAEHGLIDTPKVTVPMTIWWLIPQYMLCGVSDAFAVVGLQELFYD 488

Query: 485 QMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGSRGRWLEDNLNRSSLHYFYWVLAAL 521
           QMP+SMRS+G+AAYIS+IG+GNFLS+A+IS VQA SR +WL DNLNRS L YFYWVLA L
Sbjct: 489 QMPESMRSIGSAAYISVIGIGNFLSTAIISAVQAASRHKWLVDNLNRSKLQYFYWVLAGL 548

BLAST of HG10015724 vs. TAIR 10
Match: AT3G54450.1 (Major facilitator superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-100
Identity = 211/546 (38.64%), Postives = 313/546 (57.33%), Query Frame = 0

Query: 29  GGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSALFPLLG 88
           GGW AA+FII VE+AE+FAF GL+SNLI + T    +    AAK +N W+GVS +FP+LG
Sbjct: 14  GGWNAALFIIVVEIAERFAFYGLASNLITFLTNELGQSTATAAKNINTWIGVSCMFPILG 73

Query: 89  AFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIGAPHRKPLFFFALYILSLGEGGHRP 148
           AF+ADS+LGRFKT+++ S IY LG+V+L +S T++    R+ +FF ALY++++GEGGH+P
Sbjct: 74  AFLADSILGRFKTVLLTSFIYLLGIVMLPLSVTVVARRMREKVFFMALYVMAVGEGGHKP 133

Query: 149 CVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFLVIYVQDNIGWGLSFGILA 208
           CV TFAADQF E + E++  K+SFFN+WY+ +V+ S+ AV  +I++Q+ + W L F I+A
Sbjct: 134 CVMTFAADQFGEANAEEKAAKTSFFNYWYMAIVLASSIAVLALIFIQERVSWSLGFSIIA 193

Query: 209 GVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVAAARKWRVDETRHEWRVCYDEDNHA 268
           G +  AI+IFL G+ KYR+QVPVGSP T++AQV+VAA +KWR+  TRH + +CY+E++  
Sbjct: 194 GSVVIAIVIFLIGIPKYRKQVPVGSPFTRVAQVMVAALKKWRLSSTRHHYGLCYEEEDEH 253

Query: 269 KNEEEGQHKLMTWVPTNQFSGASSDAHILHKA----RLHHAPLRGPPLSAPAGISSRCRR 328
           K E    +++     TNQF         L KA     + H   R P            + 
Sbjct: 254 KLESTNSNQVYLLARTNQF-------RFLDKATIIDEIDHNKNRNPWRLCTVNQVEEVKL 313

Query: 329 PHHPSHRPLLRPRFCPRRQKLYRPPLRHNSATKNRNG----------------------P 388
                   +    FC    +L    L+  S      G                      P
Sbjct: 314 ILRLIPIWISLIMFCATLTQLNTFFLKQGSMMDRTIGNHFTIPPAAFQSIVGVTILILIP 373

Query: 389 IH----------LHPHHGRL----RAGRG-------------QKGNGRCRARPHRHAESD 448
           ++          +  HH  +    R G G              +      AR H   +S 
Sbjct: 374 LYDRVFVPMVRKITNHHSGITSLQRIGVGLFVATFNMVICGLVEAKRLKVARDHGLIDSP 433

Query: 449 SS---------------NGVSDAFAIIGLQELFYDQMPQSMRSLGAAAYISIIGVGNFLS 504
                             G+ D F I+G+QELFYDQMP++MRS+GAA +IS++GVG+F+S
Sbjct: 434 KEVVPMSSLWLLPQYILVGIGDVFTIVGMQELFYDQMPETMRSIGAAIFISVVGVGSFVS 493

BLAST of HG10015724 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 266.2 bits (679), Expect = 5.7e-71
Identity = 194/568 (34.15%), Postives = 280/568 (49.30%), Query Frame = 0

Query: 5   PIIKLPKKFPHHPTASNSPTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFH 64
           P++ +   + + P   +S      GGW++A FII VEVAE+FA+ G+SSNLI Y T    
Sbjct: 13  PLLAVTVDYRNKPAVKSS-----SGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLG 72

Query: 65  EPVGEAAKRVNNWVGVSALFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG 124
           +    AA  VN W G ++L PLLGAF+ADS LGRF+TI+ AS +Y +G+ +LT+SA +  
Sbjct: 73  QSTAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPS 132

Query: 125 -----------APHRKPL-FFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSF 184
                      +P  + + FF ALY+++L +GGH+PCVQ F ADQFDE  PE+ K KSSF
Sbjct: 133 DCKVSNLLSSCSPRFQVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKAKSSF 192

Query: 185 FNWWYVGLVVGSTFAVFLVIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQV--P 244
           FNWWY G+  G+   ++++ Y+QDN+ W L FGI    +  A+++ L G   YR  +   
Sbjct: 193 FNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFSIRRE 252

Query: 245 VGSPLTKIAQVVVAAARKWRVDETRHEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFS-- 304
             SP  +I  V VAA + W V             D  A  E  G   L++   + QFS  
Sbjct: 253 DQSPFVRIGNVYVAAVKNWSVSAL----------DVAAAEERLG---LVSCSSSQQFSFL 312

Query: 305 ---------GASSDAHILHKARLHHAPL------------RGPPLSAPAGIS-SRCRRPH 364
                      S D     K+ L  AP+            + P      G +  R   P 
Sbjct: 313 NKALVAKNGSCSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPG 372

Query: 365 HPSHRPLLRP-------RFCPRRQKLYRPPLRHNSATKNRNGPIHLHPHHGRLRAGRG-- 424
           +      L+         F P   ++  P  R  S T    G   L       R G G  
Sbjct: 373 YKISPATLQSFISLSIVIFIPIYDRVLIPIAR--SFTHKPGGITMLQ------RIGTGIF 432

Query: 425 -----------QKGNGRCRARPHRHAESDSSN---------------GVSDAFAIIGLQE 484
                       +      A  +   +S  +                G++D FA++GLQE
Sbjct: 433 LSFLAMVVAALVEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQE 492

Query: 485 LFYDQMPQSMRSLGAAAYISIIGVGNFLSSAVISIVQAGS----RGRWLEDNLNRSSLHY 496
            FYDQ+P  +RS+G A Y+SI G+GNFLSS +ISI++  +    +  W  +NLN++ L Y
Sbjct: 493 FFYDQVPNELRSVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDY 552

BLAST of HG10015724 vs. TAIR 10
Match: AT3G53960.1 (Major facilitator superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 8.5e-67
Identity = 188/575 (32.70%), Postives = 280/575 (48.70%), Query Frame = 0

Query: 23  PTMPPGGGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSA 82
           P     G W+AA+FII +E +E+ ++ G+S+NL++Y TT+ H+ +  A K  N W GV+ 
Sbjct: 33  PLRAQTGAWRAALFIIGIEFSERLSYFGISTNLVVYLTTILHQDLKMAVKNTNYWSGVTT 92

Query: 83  LFPLLGAFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLIG--APH----------RKP 142
           L PLLG F+AD+ LGR+ T+++A+ IY +G++LLT+S  + G  A H           + 
Sbjct: 93  LMPLLGGFVADAYLGRYGTVLLATTIYLMGLILLTLSWFIPGLKACHEDMCVEPRKAHEI 152

Query: 143 LFFFALYILSLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFL 202
            FF A+Y++S+G GGH+P +++F ADQF++  PE+RK K S+FNWW  GL  G   AV +
Sbjct: 153 AFFIAIYLISIGTGGHKPSLESFGADQFEDGHPEERKMKMSYFNWWNAGLCAGILTAVTV 212

Query: 203 VIYVQDNIGWGLSFGILAGVLAAAIIIFLAGVKKYRRQVPVGSPLTKIAQVVVAAARKWR 262
           ++Y++D IGWG++  IL  V+A +  IF  G   YR + P GSPLT + QV VAA  K  
Sbjct: 213 IVYIEDRIGWGVASIILTIVMATSFFIFRIGKPFYRYRAPSGSPLTPMLQVFVAAIAKRN 272

Query: 263 V----------DETRHEW------------------RVCYDEDNHAKNEEEGQHKLMT-- 322
           +          + T  E+                   V  D + + K E++   +L T  
Sbjct: 273 LPCPSDSSLLHELTNEEYTKGRLLSSSKNLKFLDKAAVIEDRNENTKAEKQSPWRLATVT 332

Query: 323 --------------WVPTNQFS--GASSDAHILHKARLHHAPLRGPP-LSAPAGISSRCR 382
                         W  T  F      S    + +A +    + G   +  PA + S   
Sbjct: 333 KVEEVKLLINMIPIWFFTLAFGVCATQSSTLFIKQAIIMDRHITGTSFIVPPASLFSLI- 392

Query: 383 RPHHPSHRPLLRPRFCPRRQKLYRPPLRHNSATKNRNGPIHLHP---------------- 442
                    L         +KL  P LR   AT N  G   L                  
Sbjct: 393 --------ALSIIITVTIYEKLLVPLLRR--ATGNERGISILQRIGVGMVFSLFAMIIAA 452

Query: 443 --HHGRLRAGRGQKGNGRCRARPHRHAESDSSNGVSDAFAIIGLQELFYDQMPQSMRSLG 502
                RL   +    N          A      GV+DAF ++GLQE FYDQ+P SMRSLG
Sbjct: 453 LIEKKRLDYAKEHHMNKTMTLSAIWLAPQFLVLGVADAFTLVGLQEYFYDQVPDSMRSLG 512

Query: 503 AAAYISIIGVGNFLSSAVISI----VQAGSRGRWLEDNLNRSSLHYFYWVLAALSALNLC 513
            A Y+S++G  +F+++ +I++     +  S   W   +LN S L  FYW+LAAL+A N+C
Sbjct: 513 IAFYLSVLGAASFVNNLLITVSDHLAEEISGKGWFGKDLNSSRLDRFYWMLAALTAANIC 572

BLAST of HG10015724 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.9e-66
Identity = 172/547 (31.44%), Postives = 268/547 (48.99%), Query Frame = 0

Query: 29  GGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSALFPLLG 88
           G W+AA+FII VEVAE+FA+ G+ SNLI Y T    E    AA  VN W G++ L P+LG
Sbjct: 31  GRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPLGESTAVAAANVNAWSGIATLLPVLG 90

Query: 89  AFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLI---------GAPHRKPLFFFALYIL 148
           AF+AD+ LGR++TIII+SLIY LG+  LT+SA LI          +     LFFF+LY++
Sbjct: 91  AFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLIPNTTEVTSSTSSFLNVLFFFSLYLV 150

Query: 149 SLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFLVIYVQDNIG 208
           ++G+ GH+PCVQ F ADQFDE   +++  +SSFFNWWY+ L  G  FA+ +V+Y+Q+   
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKDSQEKSDRSSFFNWWYLSLSAGICFAILVVVYIQEEFS 210

Query: 209 WGLSFGILAGVLAAAIIIFLAGVKKY----RRQVPVGSPLTKIAQVVVAAARKWRVD--- 268
           W   FGI    +  ++++F++G + Y    RR     +P T+I +V   A +  R+    
Sbjct: 211 WAFGFGIPCVFMVISLVLFVSGRRIYRYSKRRHEEEINPFTRIGRVFFVALKNQRLSSSD 270

Query: 269 --------ETRHEWRVCYDE-----------DNHAKNEEEGQ-----HKLMTW------- 328
                    T  E +  +++           +N +K+ +          +  W       
Sbjct: 271 LCKVELEANTSPEKQSFFNKALLVPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYA 330

Query: 329 VPTNQF------SGASSDAHILHKARLHHAPLRGPPLSAPAGISSRCRRPHHPSHRPLLR 388
           +P  Q+       G + D  IL   ++       PP S    I             P+  
Sbjct: 331 IPYAQYMTFFTKQGVTMDRTILPGVKI-------PPASLQVFIGISI-----VLFVPIYD 390

Query: 389 PRFCPRRQKLYRPPLRHNSATKNRNG--------PIHLHPHHGRLRAGRGQKGNGRCRAR 448
             F P  + + + P    +  +   G         I       RL             A+
Sbjct: 391 RVFVPIARLITKEPCGITTLKRIGTGIVLSTITMVIAALVEFKRLET-----------AK 450

Query: 449 PHRHAESDSSN---------------GVSDAFAIIGLQELFYDQMPQSMRSLGAAAYISI 496
            H   +   +                G++D + ++G+QE FY Q+P  +RS+G A Y+S 
Sbjct: 451 EHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVPTELRSIGLALYLSA 510

BLAST of HG10015724 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 247.7 bits (631), Expect = 2.1e-65
Identity = 177/540 (32.78%), Postives = 265/540 (49.07%), Query Frame = 0

Query: 29  GGWKAAIFIIFVEVAEQFAFIGLSSNLIMYFTTVFHEPVGEAAKRVNNWVGVSALFPLLG 88
           G W+AA FII VEVAE+FA  G+ SNLI Y T    +    AA  VN W G+S + PLLG
Sbjct: 31  GRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQSTAVAAANVNAWSGISTILPLLG 90

Query: 89  AFIADSLLGRFKTIIIASLIYCLGMVLLTVSATLI---------GAPHRKPLFFFALYIL 148
           AF+AD+ LGR+ TIIIAS IY LG+  LT+SA LI          +     LFFF+LY++
Sbjct: 91  AFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNNTEVTSSPSSFLNALFFFSLYLV 150

Query: 149 SLGEGGHRPCVQTFAADQFDEDSPEDRKKKSSFFNWWYVGLVVGSTFAVFLVIYVQDNIG 208
           ++G+ GH+PCVQ F ADQFDE +P++   +SSFFNWWY+ +  G   A+ +V+Y+Q+N+ 
Sbjct: 151 AIGQSGHKPCVQAFGADQFDEKNPQENSDRSSFFNWWYLSMCAGIGLAILVVVYIQENVS 210

Query: 209 WGLSFGILAGVLAAAIIIFLAGVKKYR----RQVPVGSPLTKIAQVVVAAARKWRVDETR 268
           W L FGI    +  ++++F+ G K YR    RQ    +P T+I +V   A +  R++ + 
Sbjct: 211 WALGFGIPCVFMVISLVLFVLGRKSYRFSKTRQEEETNPFTRIGRVFFVAFKNQRLNSS- 270

Query: 269 HEWRVCYDEDNHAKNEEEGQHKLMTWVPTNQFSGASSDAHILHKAR-------------- 328
               +C  E   A   +E   +L            S +  +  K+R              
Sbjct: 271 ---DLCKVELIEANRSQESPEELSFLNKALLVPNDSDEGEVACKSRDVEDATALVRLIPV 330

Query: 329 ----LHHAPLRGPPLSAPAGISSRCRRPHHP-------SHRPLLR---PRFCPRRQKLYR 388
               L +A      ++          R   P       S + L+      F P   ++  
Sbjct: 331 WLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVEIPPASLQVLISISIVLFVPIYDRVLV 390

Query: 389 PPLRHNSATKNRNGPIHLHPHHGRLRAGRGQ-------------KGNGRCRARPHRHAES 448
           P  R  S TK+  G   L       R G G              +      A+ +   + 
Sbjct: 391 PIGR--SITKDPCGITTLK------RIGTGMVLATLTMVVAALVESKRLETAKEYGLIDQ 450

Query: 449 DSSN---------------GVSDAFAIIGLQELFYDQMPQSMRSLGAAAYISIIGVGNFL 496
             +                G++D   ++G+QE FY Q+P  +RSLG A Y+S +GVG+ L
Sbjct: 451 PKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTELRSLGLAIYLSAMGVGSLL 510